CN104224631A - Plant extract composition applicable to androgenetic alopecia, and preparation method and application thereof - Google Patents
Plant extract composition applicable to androgenetic alopecia, and preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to the field of daily articles, in particular to a plant extract composition applicable to androgenetic alopecia, and a preparation method and application thereof. The plant extract composition comprises a sabal fruit extract, an impatiens balsamina extract, a scutellaria baicalensis extract, a radix sophorae flavescentis extract, a fructus cnidii extract, a ginseng extract, an angelica extract and a pepper extract, and the balance of an ethanol solution, wherein each extract accounts for 0.1-5% of the total weight of the composition. The plant extract composition can take 5alpha-reductase and an androgen receptor as targets simultaneously, can effectively control and eliminate microbial infection of a scalp, has an anti-inflammatory effect, can effectively treat male alopecia seborrhoeica caused by male androgens under a synergistic action of Chinese herbal prescriptions capable of increasing blood microcirculation of the scalp, and reduces the probability of male early hair loss and alopecia areata. By the preparation method, active components in the extracts can be effectively protected, and the active component content of the plant extract composition is 4-20 times higher than that in the conventional method.
Description
Technical field
The present invention relates to household chemicals field, be specifically related to a kind of be applicable to androgenetic seborrheic alopecia plant extract compositions, preparation method and application thereof.
Background technology
Seborrheic alopecia, has another name called male pattern alopecia, androgenetic alopecia, often shows as the gradual alopecia that hair is greasy, consider more, have obvious scratchiness, E Nie district and top to be worth doing, then forms great number.The male being apt to occur in 20 ~ 30 years old is between twenty and fifty, also known as male pattern alopecia.Seborrheic alopecia is clinical commonly encountered diseases, the frequently-occurring disease of department of dermatologry, is also one of refractory disease.Because primary disease hinders appearance, affect attractive in appearance, often bring very large stress and psychological burden to patient, therefore the research of seborrheic alopecia is paid attention to widely.
Modern medicine study display inherited genetic factors is the one of the main reasons that seborrheic alopecia occurs, and heredodiathesis makes hair increase androgenic biological action sensitivity; And Hyperandrogenism or to increase (quantity of androgen receptor and expression intensity) to Androgen-sensitive be this pathogenetic principal element, 5α-reductase is abnormal has important association with this disease.Except foreign genetic element, long-term psychentonia, drinking and eating irregularly, psychological unbalance and courses of infection (as Malassezia furfur, propionibacterium acnes etc.) are for bringing out or increase the weight of the key factor of primary disease.
Therapeutic scheme for seborrheic alopecia comprises doctor trained in Western medicine and tcm therapy, and doctor trained in Western medicine, mainly through antagonism androgenic endo-medicine finasteride antagonism androgen and topical minoxidil tincture, exists that user is feminized, the side effect such as skin irritation is large.Chinese traditional treatment mainly employs Traditional Chinese medical theory and carries out Chinese herbal treatment, current existing invention is mainly based on differential diagnosis of diseases and the dialectical principle that combines of Chinese traditional treatment alopecia, prescription is carried out by the natural plant of screening nourishing blood to tranquillize the mind, dispersing the stagnated live-QI to relieve the stagnation of QI, QI invigorating hair growth promoting, but these Chinese herb compounds or related preparations are seldom simultaneously for the comprehensive target spot that 5α-reductase activity, androgen receptor and related microorganisms infect, and seldom while taking into account these action target spots, to the suppression of the high Malassezia furfur that detects of wherein seborrheic alopecia and propionibacterium acnes propagation.
Chinese herbal medicine and general plant compound recipe extract in the past, take the method for high temperature infusion to obtain often, or take ethanol to soak the method for (as soaked 1 month) then vacuum distillation acquisition extractum for a long time.The former is by high temperature infusion, often make the weight polyphenol fraction in Chinese herbal medicine or plant compound recipe be oxidized in a large number, starch gelatinization, cause a kind of black and sticky mastic, be unfavorable for making as formula such as tinctures and use, and its effective ingredient is also difficult to be released in efficiently in the mastic of infusion.The alcohol-pickled method of rear one then needs long operation, and efficiency is lower.
Summary of the invention
The present invention's first object lacks in seborrheic alopecia treatment the deficiency that Mutiple Targets effectively controls alopecia for existing Chinese and western medicine, the plant extract compositions being applicable to androgenetic seborrheic alopecia of the present invention, can suppress for 5α-reductase activity simultaneously, the antagonism target spot of androgen receptor, and to causing the major microorganisms of seborrheic alopecia as aerobic fungus (Malassezia furfur), Anaerobic Bacteria (propionibacterium acnes) and facultative aerobic bacteria (staphylococcus aureus and staphylococcus epidermidis) produce and effectively suppress, thus reduce seborrheic alopecia odds, increase the cooperative effect for the treatment of corresponding symptom to other medicaments and chemicals.
Another object of the present invention is the deficiency for existing extracting method, and the method taked is cryogenic high pressure difference extractive technique, and the method can effective ingredient in available protecting extract, and the comparable traditional method of its active component content exceeds 4-20 doubly.Obtain extracting solution by various level filter membrane filter, to reduce unwanted protein, pigment and the microorganism impact on extracting solution product phase.
Another object of the present invention is to provide Anti-hair loss household chemicals prepared by the plant extract compositions that is applicable to androgenetic seborrheic alopecia described in a kind of application.
In order to realize above object, the invention provides following technical scheme:
A kind of plant extract compositions being applicable to androgenetic seborrheic alopecia, it comprises saw palmetto berry, Flos Impatientis, Radix Scutellariae, Radix Sophorae Flavescentis, Fructus Cnidii, Radix Ginseng, Radix Angelicae Sinensis and Fructus Zanthoxyli extract, wherein, relative to the compositions of 100 weight portions, the percentage composition that described each extract accounts for total composition is respectively 0.1 ~ 10 part, and surplus is alcoholic solution.
As preferably, the percentage composition that described each extract accounts for total composition is respectively 1 ~ 3 part.
Be applicable to a preparation method for the plant extract compositions of androgenetic seborrheic alopecia, it comprises following steps:
A. pulverize, dissolve: dried by above-mentioned various raw material, carry out pulverizing and cross 200 mesh sieves, be that 20 ~ 80% ethanol dissolve as solvent using concentration, the weight ratio of raw material and solvent is 1:3 ~ 1:10;
B. cryogenic high pressure difference is extracted: mixture a step obtained is under the low temperature environment of 5 ~ 50 DEG C, and cause the plant cell of compound recipe broken by producing pressure differential instantaneously, its active component overflows and comes in contact with solvent and obtain;
C. Using Multistage Membranes filters: wherein, filtration temperature is 14 ~ 20 DEG C, and pressure is 0.02 ~ 0.1mPa, and flow velocity is 1.5 ~ 3LPM, and motor modulation frequency is 45 ~ 50Hz.
As preferably, in described step b, Extracting temperature is 20 ~ 40 DEG C, and pressure is 28 ~ 33mPa, and flow velocity is 6 ~ 7.5LPM, and motor modulation frequency is 50Hz.
A kind of Anti-hair loss household chemicals, it is made up of the additive of described plant extract compositions and compound recipe, and the content of plant extract compositions is 4 ~ 32 parts, and the content of the additive of compound recipe is 68 ~ 96 parts.
Wherein, the additive of described compound recipe is made up of one or more in diluent, wetting agent, thickening agent, surfactant, increasing penetrating agent, antiseptic, pH adjusting agent, antibacterial, stabilizing agent, buffer agent, excipient, spice, dyestuff.
The Classification system of herbal raw material of the present invention and action and efficacy thereof are as following table 1.
The Classification system of table 1 herbal raw material and action and efficacy thereof
Compared with prior art, the beneficial effect that the present invention has is:
The plant extract compositions being applicable to androgenetic seborrheic alopecia of the present invention can simultaneously for 5a-reductase, androgen receptor as target spot, and effectively can control, eliminate scalp infected by microbes and anti-inflammatory effect, the microcirculatory herbal prescription of collaborative increase scalp blood, effectively can treat the seborrheic alopecia because male androgen source property causes, reduce the probability of the early stage alopecia of male, alopecia areata.Its pure Chinese medicinal preparation meets cosmetics health specification and sterile products management regulation, and preparation is simple, production cost is low and use conveniently, can be used in the formula of caused by cosmetics for special use and sterile products, as anticreep tincture, anticreep essence, anticreep shampoo etc.; Preparation method can effective ingredient in available protecting extract, and the comparable traditional method of its active component content exceeds 4-20 doubly.Obtain extracting solution by various level filter membrane filter, to reduce unwanted protein, pigment and the microorganism impact on extracting solution product phase.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in further detail, but any limitation of the invention do not formed.
The plant extract compositions being applicable to androgenetic seborrheic alopecia of embodiment 1 ~ 4 is as shown in table 2.
Table 2
| Component (gram) | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 |
| Saw palmetto berry | 100 | 50 | 25 | 10 |
| Flos Impatientis | 100 | 100 | 50 | 25 |
| Radix Scutellariae | 50 | 30 | 25 | 10 |
| Radix Sophorae Flavescentis | 50 | 50 | 25 | 25 |
| Fructus Cnidii | 100 | 75 | 50 | 20 |
| Radix Ginseng | 50 | 50 | 30 | 30 |
| Radix Angelicae Sinensis | 75 | 50 | 25 | 25 |
| Pericarpium Zanthoxyli | 20 | 30 | 20 | 20 |
| Alcoholic solution | 455 | 565 | 750 | 835 |
| Total composition | 1000 | 1000 | 1000 | 1000 |
The preparation method being applicable to the plant extract compositions of androgenetic seborrheic alopecia of embodiment 1-4, comprises following steps:
A. pulverize, dissolve: weigh according to weight portion and dried by above-mentioned various raw material, carry out pulverizing and cross 200 mesh sieves, be that 20 ~ 80% ethanol dissolve as solvent using concentration, the weight ratio of raw material and solvent is 1:3 ~ 1:10;
B. cryogenic high pressure difference is extracted: mixture a step obtained is under the low temperature environment of 5 ~ 50 DEG C, and cause the plant cell of compound recipe broken by producing pressure differential instantaneously, its active component overflows and comes in contact with solvent and obtain;
C. Using Multistage Membranes filters: wherein, filtration temperature is 14 ~ 20 DEG C, and pressure is 0.02 ~ 0.1mPa, and flow velocity is 1.5 ~ 3 LPM, and motor modulation frequency is 45 ~ 50Hz.
Effect example 1.
The plant extract compositions being applicable to androgenetic seborrheic alopecia that respectively prepared by Application Example 1 ~ 4 is tested the inhibitory action of 5α-reductase and androgen receptor (AR), and experimental result is as shown in table 3.
Table 3
In embodiment 1-4, the content of saw palmetto berry and Flos Impatientis successively decreases successively, test finds that extract to taper off situation successively to the suppression of 5α-reductase activity, illustrates that mastery reaction is played in the activity suppression of content to 5α-reductase of saw palmetto berry in formula and Flos Impatientis.Because 5α-reductase activity is high, mean that testosterone changes the ability of active higher dihydrotestosterone in hair follicle, active high, then cause more testosterone to be converted into dihydrotestosterone, thus cause the raising of alopecia.Extract of the present invention reduces 5α-reductase activity, to the effect that alopecia has prevention and weakens.The relative expression quantity of androgen receptor means the ability of receptor to the androgenic reception such as testosterone or dihydrotestosterone, and receive androgenic ability larger, the expression of mRNA is larger, and also just the probability of meaning alopecia is larger.From embodiment 1-4, sebocyte cell androgen receptor mrna expression amount relative ratio (%) is in the effect increased progressively, mean that in embodiment, some can suppress the composition of androgen receptor to reduce as the dosage of Radix Scutellariae, Radix Sophorae Flavescentis and Fructus Cnidii etc., causes increasing the activity of androgen receptor.
Effect example 2.
The plant extract compositions being applicable to androgenetic seborrheic alopecia choosing embodiment 1 carries out fungistatic effect experiment, Bactericidal test is carried out in using forestland microorganism, wherein Malassezia furfur type strain is ATCC14521, propionibacterium acnes ATCC65102, staphylococcus aureus pattern bacterium is strains A TCC 6538.
Wherein, different cultural method is adopted for different microorganisms, as Malassezia furfur adopts fungi culture medium, shaken cultivation, propionibacterium acnes adopts Anaerobic culturel method, staphylococcus aureus then adopts LB broth bouillon shaken cultivation, and first qualification is also inoculated, after spectrophotometer turbidimetry Quantitative microbial quantity in a small amount, by it on foot in culture dish, standard Oxford cup adds proper amount of medication and contrast agents, and each strain does 4 repetitions, goes inhibition zone meansigma methods after cultivating 24h.
Reference standard bacteria inhibition assay method, inhibition zone >20mm is extremely sensitive, and 10-20mm is that severe is responsive, and <10mm is drug resistance.
Positive contrast's antibacterial that three kinds of different microorganisms use is all metronidazole, and this antibacterial is widely used in sterile products and cosmetics as conventional antibacterial.Be Gao Min by experimental data this herbal composite visible to the suppression of Malassezia furfur, quick in propionibacterium acnes and staphylococcus aureus being, be slightly better than classical antibacterial metronidazole.Illustrate except the composite optimization of this prescription for object Antimicrobial effectively except, using the cryogenic high pressure difference extracting method being different from traditional heating method, more effectively can extract the effective ingredient in Chinese herbal medicine combination, thus improving the usefulness to Antimicrobial.
Result of the test is as shown in table 4.
Table 4
| Microbe species | Malassezia furfur | Propionibacterium acnes | Staphylococcus aureus |
| Inhibition zone (contrast) mm | 22.3(18.5) | 19.3(17.2) | 18.7(9.6) |
| Fungistatic effect | Gao Min (in quick) | In quick (in quick) | In quick (hypoallergenic) |
Effect example 3.
The plant extract compositions being applicable to androgenetic seborrheic alopecia choosing embodiment 1 carries out Anti-hair loss effect experimental, use alopecia test volunteer 35 people, Hamilton alopecia classification II-IV grade is met through qualification, wherein 30 people test and use this product totally 60 days, every 10 day early morning added up alopecia quantity, simultaneously to alopecia scalp sampling determination Malassezia furfur number.Alopecia number with 30 DEG C of warm water washing hairs 5 minutes, calculates alopecia quantity by filtered through gauze with early morning.Remaining 5 people, as negative control, use mill run but not the Chinese herbal medicine formula taked of this technology, also do alopecia quantity and Malassezia furfur number statistical simultaneously.
The method of inspection of Malassezia furfur, pastes with adhesive tape of paste and produces the most serious position of the dandruff at scalp, get same area at every turn, then gets thing with sterilized water eluting is sticky, suitably dilutes, calculate colony counts after cultivating 24h with fungi culture medium.
This effect example uses the impact of patient's alopecia quantity and as shown in table 5 on the impact of scalp Malassezia furfur quantity.
Table 5
Can obviously find from above table data, said composition can slow down hair loss rate rapidly to hair loss patient after a procedure, and after 60 days, alopecia quantity decreases about 54.38%.Have obvious inhibition for scalp chlosma quantity, after 60 days, the Malassezia furfur quantity of user scalp decreases about 49% simultaneously.Therefore, compositions be anticreep or suppress prescribed microorganism effect all clearly.
The above embodiments are only the preferred embodiments of the present invention, can not limit interest field of the present invention with this, and therefore, the equivalent variations done according to the present patent application the scope of the claims, still belongs to the scope that the present invention is contained.
Claims (6)
1. one kind is applicable to the plant extract compositions of androgenetic seborrheic alopecia, it is characterized in that: comprise saw palmetto berry, Flos Impatientis, Radix Scutellariae, Radix Sophorae Flavescentis, Fructus Cnidii, Radix Ginseng, Radix Angelicae Sinensis and Fructus Zanthoxyli extract, wherein, relative to the compositions of 100 weight portions, the percentage composition that described each extract accounts for total composition is respectively 0.1 ~ 10 part, and surplus is alcoholic solution.
2. the plant extract compositions being applicable to androgenetic seborrheic alopecia according to claim 1, is characterized in that: the percentage composition that described each extract accounts for total composition is respectively 1 ~ 3 part.
3. be applicable to a preparation method for the plant extract compositions of androgenetic seborrheic alopecia, it is characterized in that: comprise following steps:
A. pulverize, dissolve: dried by above-mentioned various raw material, carry out pulverizing and cross 200 mesh sieves, be that 20 ~ 80% ethanol dissolve as solvent using concentration, the weight ratio of raw material and solvent is 1:3 ~ 1:10;
B. cryogenic high pressure difference is extracted: mixture a step obtained is under the low temperature environment of 5 ~ 50 DEG C, and cause the plant cell of compound recipe broken by producing pressure differential instantaneously, its active component overflows and comes in contact with solvent and obtain;
C. Using Multistage Membranes filters: wherein, filtration temperature is 14 ~ 20 DEG C, and pressure is 0.02 ~ 0.1mPa, and flow velocity is 1.5 ~ 3LPM, and motor modulation frequency is 45 ~ 50Hz.
4. the preparation method being applicable to the plant extract compositions of androgenetic seborrheic alopecia according to claim 3, it is characterized in that: in described step b, Extracting temperature is 20 ~ 40 DEG C, and pressure is 28 ~ 33mPa, flow velocity is 6 ~ 7.5LPM, and motor modulation frequency is 50Hz.
5. an Anti-hair loss household chemicals, is characterized in that: it is made up of the additive of described plant extract compositions and compound recipe, and the content of plant extract compositions is 4 ~ 32 parts, and the content of the additive of compound recipe is 68 ~ 96 parts.
6. Anti-hair loss household chemicals according to claim 5, is characterized in that: the additive of described compound recipe is made up of one or more in diluent, wetting agent, thickening agent, surfactant, increasing penetrating agent, antiseptic, pH adjusting agent, antibacterial, stabilizing agent, buffer agent, excipient, spice, dyestuff.
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| CN112912058A (en) * | 2018-10-17 | 2021-06-04 | 鸵鸟制药株式会社 | Antibodies for improving hair |
| CN113288853A (en) * | 2021-03-02 | 2021-08-24 | 本草生活(北京)医学研究有限公司 | Natural composition tincture for nourishing hair follicles, preventing alopecia, promoting hair growth, resisting inflammation and resisting bacteria and preparation method thereof |
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