CN104215774B - A kind of method utilizing FGF21 factor diagnosis of milk cow sub-clinical ketosis - Google Patents
A kind of method utilizing FGF21 factor diagnosis of milk cow sub-clinical ketosis Download PDFInfo
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Abstract
A kind of method utilizing FGF21 factor diagnosis of milk cow sub-clinical ketosis.The FGF21 factor and milk cow recessive ketoacidosis are proportionate, cow serum index when application ELISA kit and Biochemical Analyzer measure FGF21 factor concentration and ketoacidosis, sub-clinical ketosis in cow's serum, by statistical analysis FGF21 and sub-clinical ketosis in obviously relevant, with clinical ketoacidosis non-correlation; As 335ng/ml & lt; FGF21 & lt; Milk cow recessive ketoacidosis can be diagnosed as during 443ng/ml.The method determines that FGF21 and milk cow recessive ketoacidosis are proportionate, and cow serum FGF21 can substitute BHBA diagnosis of milk cow sub-clinical ketosis, therefore, it is possible to monitoring comparatively early goes out the loss that sub-clinical ketosis will reduce milk cow production.
Description
Technical field
The present invention relates to a kind of method of diagnosis of milk cow sub-clinical ketosis, particularly a kind of method utilizing FGF21 factor diagnosis of milk cow sub-clinical ketosis.
Background technology
Milk cow production is one of China's animal husbandry industry pillar, but ketosis of dairy cows brings comparatively serious financial consequences to dairy.Especially milk cow recessive ketoacidosis, because sub-clinical ketosis clinical symptoms is not obvious, this just brings great difficulty to Diagnosis and treatment, and therefore comparatively the monitoring of morning goes out the loss that sub-clinical ketosis will reduce milk cow production.
FGF21 is one of fibroblast growth family member.Current research shows, FGF21 stimulates adipocyte ingestion of glucose, and does not have this to act on to other cell type, and this effect is actually and is equivalent to enhance insulin active.After obesity mice injection FGF21, adipose tissue Glucose transporter-4 significantly increases.Under Diet-Induced Obesity, hypoglycemia and high triglyceride level condition, FGF21 has protective effect to diabetic rats.Further result of study shows, after animal injection FGF21, causes energy ezpenditure to increase, and fat utilization and lipid excretion are accelerated.And have FGF21 can be used as the report of Diagnosis of fatty.
The monitoring of current sub-clinical ketosis is mainly according to BHBA concentration change, and diagnosable as 1.2mmol/L<BHBA<1.6mmol/L is milk cow recessive ketoacidosis, but this kind of method susceptibility is lower, easily causes and fails to pinpoint a disease in diagnosis.Therefore need to find the monitoring that the higher factor of sensitivity carries out milk cow recessive ketoacidosis.
Summary of the invention
The invention provides a kind of method utilizing FGF21 factor diagnosis of milk cow sub-clinical ketosis, described FGF21 changes more responsive to ketoboidies during ketosis of dairy cows, particularly in the milk cow recessive ketoacidosis stage, illustrate the relation between FGF21 in energetic supersession characteristics index BHBA and body, show that FGF21 and milk cow recessive ketoacidosis are proportionate.
The technical solution adopted in the present invention is: a kind of method utilizing FGF21 factor diagnosis of milk cow sub-clinical ketosis, the FGF21 factor and milk cow recessive ketoacidosis closely related, cow serum index when application ELISA kit and Biochemical Analyzer measure FGF21 factor concentration and ketoacidosis, sub-clinical ketosis in cow's serum, by statistical analysis FGF21 and sub-clinical ketosis in obviously relevant, with clinical ketoacidosis non-correlation; Milk cow recessive ketoacidosis can be diagnosed as 335ng/ml<FGF21<443ng/ml.
Serum analysis FGF21 metabolic index in such scheme and clinical ketoacidosis group, sub-clinical ketosis group, health organize main testing result, clinical ketoacidosis group, and sub-clinical ketosis group and healthy group serum FGF21 have statistical significance, P<0.05; Healthy group, sub-clinical ketosis group, clinical ketoacidosis group compare BHBA in serum and have statistical significance, P<0.05; During sub-clinical ketosis, the serum FGF21 factor and BHBA are proportionate, r=0.659, p=0.005; During clinical ketoacidosis, the serum FGF21 factor and BHBA are without relevant r=0.005, p=0.979.
The beneficial effect that the present invention has is: the present invention is based on above-mentioned background and pass through the clinical ketoacidosis of milk cow, in sub-clinical ketosis and healthy cow blood, FGF21 should to mesostate BHBA horizontal reverse important during ketosis of dairy cows, FGF21 Changing Pattern in Contents in Cows when specifying sub-clinical ketosis, measure the metabolic index in cows sample and the FGF21 factor, determine that FGF21 and milk cow recessive ketoacidosis are proportionate, cow serum FGF21 can substitute BHBA diagnosis of milk cow sub-clinical ketosis, can determine that as 335ng/ml<FGF21<443ng/ml milk cow is sub-clinical ketosis, therefore, it is possible to monitoring comparatively early goes out the loss that sub-clinical ketosis will reduce milk cow production.
Embodiment
Below in conjunction with experiment, the present invention is further described:
Zoopery: milk cow is all from Mishan cattle farm, Heilongjiang Province, and age, parity no significant difference between milk cow, all cow in perinatal period TMR feed.BHBA>1.2mmol/L is as diagnosis of milk cow ketoacidosis standard, if BHBA>1.6mmol/L in cow serum, and shows obvious clinical symptoms, is diagnosed as clinical ketoacidosis group; If 1.2mmol/L<BHBA<1.6mmol/L, and there is no obvious clinical symptoms, be diagnosed as sub-clinical ketosis group; BHBA<1.2mmol/L and without any clinical symptoms performance, as healthy group.Wherein healthy 30, recessive 30, clinical 30.In addition, wherein suffer from mastitis, hysteritis, miscarriage, due to drug induced hepatopathy, the milk cow of the diseases such as heating is excluded this test.
Sample collection and process
All milk cows all extract tail vein on an empty stomach morning, and do not add anti-coagulants, be placed in by sampling test tube among ice chest, be brought back laboratory within 1h, the centrifugal 15min of 3000g, saves backup at separation of serum-20 DEG C.
Index determining method: FGF21 uses RD bovine fibroblasts factor ELISA kit, and all the other metabolic index measure respectively on automatic biochemistry analyzer.
Data analysis: all market demand IBMSPSS19.0 software is analyzed, measurement data mean with
represent, between group, the average significance of difference adopts the analysis of single factor test variance statistic, and correlation analysis adopts Pearson linear correlation to analyze.P<0.05 is significant correlation.
Experimental result:
The FGF21 of the routine milk cow of one-way analysis of variance 90, blood sugar, BHBA, NEFA testing result is in table 1.
The main Testing index situation of table 1 cow serum
The correlation analysis of Person correlation analysis FGF21 metabolic index and ketoacidosis is in table 2.Serum FGF21 and NEFA is proportionate, and is proportionate with BHBA.
Table 2 milk cow FGF21 and serum leading indicator correlation analysis
The relation of the dissimilar ketoacidosis FGF21 of one-way analysis of variance and ketoacidosis metabolic index, is respectively clinical ketoacidosis 30 example in 90 routine milk cows, sub-clinical ketosis 30 example, healthy ox 30 example.3 groups of cows parity, age are close, and serum FGF21 metabolic index and the main testing result of ketosis of dairy cows are in table 3.Clinical ketoacidosis group, sub-clinical ketosis group and healthy group serum FGF21 (P<0.05), have statistical significance; Healthy group, sub-clinical ketosis group, clinical group of serum N EFA and BHBA comparison in difference (P<0.05), have statistical significance.
The comparison of table 33 group milk cows FGF21 and the main Testing index of serum
The correlation analysis of serum FGF21 metabolic index and ketoacidosis index that 3 groups of cows are analyzed in Person linear correlation is in table 4.Sub-clinical ketosis group serum FGF21 and BHBA is proportionate; Clinical ketoacidosis group serum FGF21 and BHBA non-correlation.
The correlation analysis of table 4 different cohort FGF21 metabolic index and ketoacidosis index
FGF21 and the important Testing index BHBA of recessive ketosis of dairy cows is proportionate, FGF21 can be used for alternative BHBA as the new factor of diagnosis of milk cow sub-clinical ketosis, can be diagnosed as milk cow recessive ketoacidosis as 335ng/ml<FGF21<443ng/ml.
Claims (2)
1. the purposes in milk cow recessive ketoacidosis diagnostic reagent prepared by the reagent measuring FGF21, it is characterized in that: the FGF21 factor and milk cow recessive ketoacidosis closely related, all the other metabolic index of cow serum when application ELISA kit and Biochemical Analyzer measure FGF21 factor concentration and ketoacidosis, sub-clinical ketosis in cow serum, by statistical analysis FGF21 and sub-clinical ketosis in obviously relevant, with clinical ketoacidosis non-correlation; Milk cow recessive ketoacidosis can be diagnosed as 335ng/ml<FGF21<443ng/ml.
2. purposes according to claim 1, it is characterized in that: serum analysis FGF21 metabolic index and clinical ketoacidosis group, sub-clinical ketosis group, health organize main testing result, clinical ketoacidosis group, sub-clinical ketosis group and healthy group serum FGF21 have statistical significance, P<0.05; Healthy group, sub-clinical ketosis group, clinical ketoacidosis group compare, and in serum, BHBA has statistical significance, P<0.05; During sub-clinical ketosis, the serum FGF21 factor and BHBA are proportionate, r=0.659, p=0.005; During clinical ketoacidosis, the serum FGF21 factor and BHBA are without relevant, r=0.005, p=0.979.
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| CN105494959A (en) * | 2015-10-16 | 2016-04-20 | 黑龙江八一农垦大学 | Health-care granules for preventing negative balance of energy metabolism of cows and using method of health-care granules |
| WO2018003638A1 (en) * | 2016-06-30 | 2018-01-04 | 味の素株式会社 | Method for evaluating postpartum ketosis |
| CN109689079A (en) * | 2016-08-22 | 2019-04-26 | 伊兰科美国公司 | Ketoacidosis in bovine fibroblasts growth factor-2 1 and milk animal |
| TW202011029A (en) * | 2018-04-04 | 2020-03-16 | 美商建南德克公司 | Methods for detecting and quantifying FGF21 |
| CN113481221A (en) * | 2021-08-27 | 2021-10-08 | 黑龙江八一农垦大学 | Coding gene SOD in blood related to milk cow ketosis and PCR detection kit thereof |
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| CN201732094U (en) * | 2009-12-28 | 2011-02-02 | 黑龙江八一农垦大学 | Dairy cow ketosis test paper strip |
| CN103792230A (en) * | 2014-01-13 | 2014-05-14 | 东北农业大学 | Dairy cow ketosis diagnostic kit and detecting method thereof |
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| CN201732094U (en) * | 2009-12-28 | 2011-02-02 | 黑龙江八一农垦大学 | Dairy cow ketosis test paper strip |
| CN103792230A (en) * | 2014-01-13 | 2014-05-14 | 东北农业大学 | Dairy cow ketosis diagnostic kit and detecting method thereof |
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| Fibroblast growth factor 21-deficient mice demonstrate impaired adaptation to ketosis;Badman M K.et al.;《Endocrinology》;20091130;第150卷(第11期);4931-4940 * |
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