CN201732094U

CN201732094U - Dairy cow ketosis test paper strip

Info

Publication number: CN201732094U
Application number: CN2009202444482U
Authority: CN
Inventors: 夏成; 张洪友; 吴凌; 李修明; 黄海波; 郑家三; 徐闯
Original assignee: Heilongjiang Bayi Agricultural University
Current assignee: Heilongjiang Bayi Agricultural University
Priority date: 2009-12-28
Filing date: 2009-12-28
Publication date: 2011-02-02
Anticipated expiration: 2019-12-28

Abstract

The utility model discloses a dairy cow ketosis test paper strip. Diagnosis of dairy cow ketosis mainly relies on detection of ketone content in a cow body, and the dairy cow ketosis test paper strip has the advantages of convenience, speediness, ease of large-area popularization and use and the like, provides an effective method for testing fairy cow urine and milk ketone content to monitor occurrence of fairy cow ketosis, increases prevention effect and reduces treatment cost.

Description

The ketosis of dairy cows test strip

Technical field

The utility model relates to the ketosis of dairy cows test strip, be used for the diagnosis of ketosis of dairy cows.

Background technology

At present, raising along with the national life level, " one glass of strong nationality of milk " fully realized by people with healthy slogan of laying equal stress on, dairy produces such as breakfast milk, high calcium milk, plain chocolate become the requisite health food of people, in order to satisfy national drink milk needs, quantity, the scale of China's raising dairy cattle constantly enlarge, and have become the mainstay industry of China's animal husbandry development.Milk production of cow significantly improve in, the dairy cow nutrition metabolic disease is also corresponding to be increased, especially ketosis of dairy cows is lactating cow the most a kind of Nutrition and Metabolism disease of normal generation, pilosity is born in high yield cow, so be in the milk cow body carbohydrates and volatile fatty acid metabolic disorder rise a kind of metabolic disease of generalized functional disorder, it is characterized in that blood, urine, Ruzhong ketoboidies content increases, blood sugar concentration descends, the digestive function disorder, lose weight, the output of milk descends, and its diagnosis and control are all had very big difficulty, all have popularly in countries in the world, become one of principal disease of raising dairy cattle industry.The early diagnosis of ketosis of dairy cows is a long-term troublesome problem of the sector with control always, and therefore seeking a kind of ketosis of dairy cows test strip becomes hoping for of vast plant, family and animal doctor.

The utility model content

(1) technical matters that will solve

The purpose of this utility model is to provide a kind of ketosis of dairy cows test strip at the problems referred to above, the diagnosis of ketosis of dairy cows is mainly by ketoboidies content in the detection bodies, have only it is implemented early diagnosis effectively, reasonably prevent and treat, could reduce the sick thus massive losses that is caused.Effectively the prevention ketoacidosis just need be urinated milk cow, ketoboidies detects in the milk, and therefore researching and developing a kind of practicality, cheapness, effective ketoacidosis test strip becomes one of gordian technique of ketosis of dairy cows clinical detection.

(2) technical scheme

For achieving the above object, the utility model adopts following technical scheme:

The paper slip of development described in the utility model is for soaking into examination, highly sensitive to the ox urine, low to milk sensitivity, analyze that reason and infiltration preparation technology, urine sample, newborn sample ingredient are different all certain relation, be convenient to clinical practice for overcoming above shortcoming, the pulvis fixed amount that will develop the color is carried out packing, makes portable test strips, characteristics such as the detection effect that promptly can satisfy newborn sample can also reach the stability height, and is easy to use.

(3) beneficial effect

The advantage and the good effect of ketosis of dairy cows test strip described in the utility model are:

1, the utlity model has convenient and swift, testing result is accurate, and be easy to large tracts of land and characteristics such as promote the use of.

2, the utility model is and detects milk cow urine, milk ketoboidies content provides a kind of effective method, to prevent the generation of ketosis of dairy cows, improves preventive effect, reduces medical expense.

Description of drawings

Fig. 1 is the structural representation of the utility model milk cow progesterone test strip;

Fig. 2 is the loam cake synoptic diagram in the utility model;

Fig. 3 is the loam cake side schematic view in the utility model;

Fig. 4 is the base side schematic view in the utility model;

Fig. 5 is the base synoptic diagram in the utility model;

Fig. 6 is the colorimetric card synoptic diagram in the utility model;

Among the figure: 1. loam cake; 2. application of sample window; 3 colour developing windows; 4. buckle projection; 5. base;

6. buckle recess; 7. placement chamber; 8. colorimetric card; 9 colour codes.

Embodiment

Below in conjunction with accompanying drawing, further describe the embodiment of ketosis of dairy cows test strip described in the utility model, but be not used for limiting protection domain of the present utility model.

The test strips that the utility model provides detects ketosis of dairy cows and also comprises the steps:

(1), the preparation of developer

The utility model ketosis of dairy cows test strip, the preparation of standard coloration solution: get the liposoluble of 1ml acetoacetate second in 15.6ml0.625mol/1NaOH, fully mixing dilutes with fresh and healthy cow's milk juice.Be equivalent to 60000ppm (mg/L), just be equivalent to 6000mg/dL.

Dilution variable concentrations colour developing liquid, with reference to the clinical detection standard, the ketoboidies titer is mixed with 10,20,40,80, five gradients of 160mg/dL.

The preparation of colour developing powder: natrium nitroferrocyanatum, natrium carbonicum calcinatum, ammonium sulfate etc. mix porphyrize by a certain percentage and are placed in the little brown bottle wide-necked bottle standby.

Colour developing: get colour developing pulvis 0.2g and place on the microslide, add three developers again, then with filter paper rapidly lid put on colour developing powder and developer mixture, observe color behind the 1min, and with the digital camera viewed color of taking pictures.

(2), the preparation of colorimetric card

Make colorimetric card 8: repeat above-mentioned operation, take the pairing color of ketoboidies titer of each concentration.Print colour band through the computer processing, be cut into the blockage of 0.5cm * 0.5cm, concentration by the ketoboidies titer sequences the image that obtains different ketoboidies concentration of standard solution from low to high, has just made the standard colour code that detects the variable concentrations ketoboidies, becomes colorimetric card 8 duplicating.

(3), the preparation of test strip housing

The utility model ketosis of dairy cows test strip housing, form by two plastic casings up and down, it is the base 5 of splendid attire colour developing powder test strips that the one side of placing chamber 7 is arranged, the one side that two windows are arranged is a loam cake 1, base 5 has six bayonet socket projections, the 4 chimeric integral body that become of six buckle recesses 6 and loam cake 1, loam cake 1 is an application of sample window 2 than macropore, aperture is colour developing window 3, with adding the placement chamber 7 that is contained in base 5 behind the accurate weighing colour developing of the balance powder, developing the color then, corresponding application of sample mouth covers with thieving paper on the powder, corresponding colour developing window covers with the middling speed quantitative filter paper, and cements with double faced adhesive tape Yan Dikuangchu, at last cover plate is got final product with corresponding the buckling well of buckle of base.

(4) preparation technology of ketosis of dairy cows test strip

This research is on the preparation principle basis of dipping test paper, utilize the accurate nitroprusside method of designing mould to detect newborn sample, it is improvement to the test paper method detection mode, a whole set of operating process is provided, colorimetric card is designed to 0,10,20,40,80 (mg/dL), have more clinical detection and function definition, by a large amount of experiments, the process Flame Image Process is the precision of refinement standard color card further.

The component ratio of adding the contained material of dress colour developing powder by adjustment can improve the sensitivity of test strips and detect developing time, and strip tilts to place can accelerate color speed.

(5) fresh newborn sample to be checked is adopted in the application of ketosis of dairy cows test strip, application of sample window 2 places that milk to be checked splashed into the ketosis of dairy cows test strip splash into newborn sample to be checked, observe the metachromasia of colour developing window 3 in one minute, with a plurality of colour codes 9 colorimetrics of colorimetric card 8, get final product result of determination then.

The technology of preparing principal feature of ketosis of dairy cows test strip is effectively to detect newborn sample, easy to usely be better than urinating ketoboidies and detect test paper, and strip is easy to assembly, preparation flow is simple, clinical practice combines the index that mensuration can be used for the subclinical ketosis early monitoring with the dipping test paper, and it is better that clinical type ketoacidosis is detected accuracy rate.Because test strips colour developing color that window 3 shows is not with instrument but detect by an unaided eye, because of everyone differs to the judgement of color, this just exists error unavoidably, influenced degree of accuracy, but it is easy and simple to handle to be to use this strip to detect ketoboidies, and accuracy, highly sensitive in ketone powder method detects when being applicable to batch samples, and the strip reserve of finished products is convenient and safe than the ketone powder, and the storage time is also long than the ketone powder.For the ketoboidies fast detecting is laid a good foundation, significant to the detection of ketosis of dairy cows.

More than be preferred forms of the present utility model, according to the disclosed content of the utility model, some identical, alternative schemes that those of ordinary skill in the art can expect apparently all should fall into the scope that the utility model is protected.

Claims

1. ketosis of dairy cows test strip, it is characterized in that: by colour developing pulvis, colorimetric card (9), test strips housing three parts, the test strips housing is combined into one by loam cake (1), base (5) by bayonet raised (4) and bayonet socket recess (6), detects box for once using.

2. ketosis of dairy cows test strip according to claim 1, it is characterized in that: have colour code (9) to sequence on the colorimetric card (8) from low to high by the concentration of ketoboidies titer, obtain the color of different ketoboidies concentration of standard solution, make colour code (9) according to color, make the standard color card (8) that detects the variable concentrations ketoboidies again.

3. ketosis of dairy cows test strip according to claim 1, it is characterized in that: it is the base (5) of splendid attire colour developing powder test strips that the test strips housing has the one side of placement chamber (7), the one side that two windows are arranged is loam cake (1), loam cake (1) is application of sample window (2) than macropore, aperture is colour developing window (3), corresponding application of sample mouth covers with thieving paper on the colour developing pulvis, and corresponding colour developing window covers with the middling speed quantitative filter paper.