CN104193842A - Method for extracting lucid ganoderma mycelia polysaccharide germanium - Google Patents
Method for extracting lucid ganoderma mycelia polysaccharide germanium Download PDFInfo
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Abstract
The invention discloses a method for extracting lucid ganoderma mycelia polysaccharide germanium. The method for extracting the lucid ganoderma mycelia polysaccharide germanium comprises the followings steps: carrying out ultrasonic disruption treatment on lucid ganoderma mycelia to obtain a solid-liquid mixture; mixing the solid-liquid mixture with activated carbon in volume ratio of 100:(1-4), carrying out heat preservation and stirring for 30-60 minutes at the temperature of 50-70 DEG C, and carrying out filter pressing, so that a first extracting solution is obtained; carrying out vacuum concentration on the first extracting solution to obtain first concentrate; dissolving the obtained first concentrate in ethyl alcohol until final concentration of ethyl alcohol in a solution is 60-70%, standing and staying overnight at room temperature, centrifuging for 20 minutes at a speed of 3000-3400rpm, so as to obtain second concentrate; continuously carrying out ultrafiltration twice, namely primary ultrafiltration and secondary ultrafiltration, on the second concentrate obtained in the step four, so that polysaccharide germanium primary extract is obtained. The lucid ganoderma mycelia polysaccharide germanium obtained by adopting the method disclosed by the invention has the characteristics of nature and no toxicity, operation is easy, and mass production can be realized.
Description
Technical field
The present invention relates to biological technical field, particularly a kind of extracting method of lucidum mycelium polysaccharide germanium.
Background technology
Glossy ganoderma is the macro fungi that a kind of pharmaceutical use is very high, has immunomodulatory, the effect such as antitumor, anticancer, and cardiovascular and cerebrovascular diseases is had to higher pharmacologically active.Recent domestic scholar has isolated more than 150 and has planted compound from glossy ganoderma, can be divided into ten large classes: polyose, organic germanium, alkaloids, aminoacid protein class, triterpenes, lipid, mineral ion etc.The active chemicals such as the polymer immune polysaccharide containing in the pharmacologically active of glossy ganoderma and glossy ganoderma, organic germanium are closely related.Therefore, research glossy ganoderma active chemical is significant.
Ganoderan is one of glossy ganoderma main active ingredient, be one of anti-ageing, main effective constituent that strengthen the body resistance to consolidate the constitution, it can strengthen the immunoregulation effect of cell, regulates the metabolic balance of nucleic acid and protein, remove oxyradical, improve the ability of subculture in vitro separately cell fission algebraically.Ganoderan can improve interleukin-II and produce, and strengthens the function of malicious T cell, and the activity of the intracellular SOD of small white mouse is had to obvious enhancement, can strengthen the activity of DNA polymerase in Aged Mice splenocyte, has certain anti-ageing ability.The immunoregulation effect of ganoderan is widely, not only comprise and improve the activity that antigen presenting cell, monocyte are engulfed system, and comprise that enhancing cellular immunization and humoral immune function, action site relate to from immune precursor cell hyperplasia, break up each process to effector cell.That ganoderan also has is antitumor, enhancing body hypoxia-bearing capability, antifatigue, radioprotective, the effect such as improve one's memory, and the health care that it has and pharmaceutical use have obtained researchist and academia is generally approved.
Recent two decades comes, and the synthetic and bioactivity research work of organic germanium compounds mainly concentrates on sesquialter germanium, is situated between on river class organic germanium, spiral shell germanium and this four compounds of porphyrin germanium.Wherein B mono-carboxyethyl germanium sesquioxide (being Ge 1) has entered the clinical study stage.Ge 1 ((GeCH: CH: COOH).03) be by 3-5 molecular low-grade polymer.It is a kind of Interferon Inducer, can be antineoplastic scavenger cell the mutagenesis of " being sunk into sleep " scavenger cell, thereby bring out the activity of cancer resistance Interferon, rabbit, and almost there is no toxicity.The highest with the activity of spiral shell germanium in spiral shell germanium and derivative thereof, synthesizing of energy arrestin matter, has obtained good effect to the treatment of various early-stage cancers.But because spiral shell germanium toxic side effect is very large, therefore be very limited in clinical application.Synthetic and the Study on Physiological Activity of sesquialter germanium and the river class organic germanium compounds that is situated between is the heat subject of recent two decades always.(2) natural organic germanium: in natural phant, fungi, extract or directly eat, without any side effects to human body.In the food plants such as coloured vegetables such as many famous and precious herbal medicine ginsengs, wolfberry fruit, monkey eye etc. and glossy ganoderma, Radix Sophorae Tonkinensis, potato, all contain a large amount of natural organic germaniums.(3) biological organic germanium: be by germanium compound implantable bioartificial body, as yeast, bacterium, macro fungi, vegetables etc., it be combined with cell a part, be converted into macromolecular cpd, make germanium-enriched yeast, Ganoderma rich in germanium etc.
Organic germanium is described as " jinx of human diseases ", " 21 century help germanium ", and it participates in internal metabolism, has very strong oxydehydrogenation ability; Have green blood hormesis, show and increase red corpuscle and amount of hemoglobin, stimulating platelet generates, and eliminates the lipid dirt settling on vessel wall, so anaemia and hypercholesterolemia blood fat and diabetes are had to certain curative effect; To some cancerous swelling, immunologic hypofunction, osteoporosis, early ageing, vascular hypertension etc., there is certain health care effectiveness.
Abroad to organic germanium research early, most study have countries such as Japan, the U.S., USSR (Union of Soviet Socialist Republics).Current, Japan, European and American countries scholar to the research of organic germanium compounds and application quite active, many scholars utilize structure activity relationship, designed dissimilar organic germanium compounds, further strengthen organic germanium compounds and there is immunity, anticancer, the research of the anti-ageing function of waiting for a long time, explores its pharmacology mechanism from molecular level, electronic horizon.Can expect, along with the further understanding of organic germanium pharmacological action and mechanism thereof, will there is important breakthrough in the disease field of the control mankind and animal in it.
The research of China's organic germanium is started late, but in recent decades, China's researcher is at organic germanium, particularly on the synthetic and bioactivity research of Ge-132 derivative and analogue thereof, done a large amount of work, many research papers have been delivered, in some research field prostatitis in the Zou world, especially about the research of antitumor action aspect.
The recent years lucidum mycelium polysaccharide germanium that begins one's study extracts, but study fewer, in these methods, patent publication No. is a kind of method that the patent of CN1687438 discloses lucidum mycelium polysaccharide germanium, but the method is passed through substratum fermentation, sterilizing, then precipitation, Direct spraying are dried, it is very low that this method obtains product purity, is kind of a mixture.The people such as old filial piety have proposed by karstl seedling filament through hot water extraction, H in 2004 glossy ganoderma symposiums
2o
2oxidative decoloration element, Sevage method are removed albumen, by DEAF:Sephadex A mono-25 separation, Sephadex G 1, are further purified, obtain a kind of way of polysaccharide.The method will be used H2O2 oxidative decoloration element, and security is not high, is not easy to realize industrialization.By Tibet Jin Ke group, in the patent of the number of applying for a patent 201210557516.9, propose another method in addition and extracted lucidum mycelium polysaccharide germanium through mycelium pre-treatment, ultrasonic extraction, ethanol precipitation, Deproteinization, refining these several procedures, this technique will be used a large amount of chloroforms in the process of Deproteinization, very large to human injury, and this technique in the end will precipitate 4 times with ethanol in exquisite process, cause this technique very complicated loaded down with trivial details, improper large explained hereafter.So, invent a kind of large production technique continuously just very necessary.
Summary of the invention
One of the object of the invention is to provide a kind of extracting method of lucidum mycelium polysaccharide germanium, the inventive method adopts ultrasonic extraction, ethanol precipitation, ultra-filtration and separation, the technique such as refining from Ganoderma rich in germanium mycelium, to extract and obtain ganoderan germanium, extract the lucidum mycelium polysaccharide germanium obtaining and there is pure natural and avirulent feature, and the inventive method is simple to operate, can realize scale operation.
For achieving the above object and some other objects, the technical solution used in the present invention is:
An extracting method for lucidum mycelium polysaccharide germanium, is characterized in that, comprises the following steps:
Step 1, Ganoderma mycelium is carried out to ultrasonic disruption process to obtain solidliquid mixture;
Step 2, by solidliquid mixture and activated carbon, be 100: 1～4 to mix by volume, insulated and stirred 30～60min at 50～70 ℃, press filtration afterwards obtains the first extracting solution;
Step 3, described the first extracting solution is carried out to vacuum concentration obtain the first enriched material;
Step 4, acquisition the first enriched material are dissolved in ethanol and make ethanol final concentration in solution reach 60～70%, room temperature standing over night, and the centrifugal 20min of 3000-3400rpm, obtains the second enriched material;
Step 5, the second enriched material obtaining is carried out to two times of ultrafiltration be continuously respectively for the first time in step 4
Ultrafiltration and for the second time ultrafiltration, obtain extract of holoside Ge.
Preferably, in step 2, solidliquid mixture and activated carbon are 100: 1～2 to mix by volume, insulated and stirred 40～50min at 60～65 ℃, and press filtration afterwards obtains the first extracting solution.
Preferably, in described step 4, obtain the first enriched material and be dissolved in ethanol and make ethanol final concentration in solution reach 70%, room temperature standing over night, the centrifugal 20min of 3000rpm, obtains the second enriched material.
Preferably, the condition of ultrafiltration is for the first time: ultra-filtration membrane aperture is 0.002～0.004 μ m, and ultrafiltration flow velocity is 3～5L/h, and ultrafiltration pressure is 0.10～0.15MPa; The condition of ultrafiltration is for the second time: ultra-filtration membrane aperture is 0.001～0.003 μ m, and ultrafiltration flow velocity is 3～5L/h, and ultrafiltration pressure is 0.3～0.5MPa.
Preferably, the condition of described ultrafiltration is for the first time: adopt 4020PS hollow-fibre membrane, ultra-filtration membrane aperture is 0.002 μ m, and ultrafiltration flow velocity is 3～5L/h, and ultrafiltration pressure is 0.10～0.15MPa; The condition of ultrafiltration is for the second time: adopt GEPW8040F poly (ether-sulfone) ultrafiltration membrane, ultra-filtration membrane aperture is 0.001 μ m, and ultrafiltration flow velocity is 3～5L/h, and ultrafiltration pressure is 0.3～0.5MPa.
Preferably, in described step 5, also comprise by the second enriched material and distilled water being 1: 8～10 to mix and carry out ultrafiltration for the first time and obtain the first ultrafiltration thing by volume, and be 1: 8～10 to mix and carry out ultrafiltration for the second time to obtain the second ultrafiltration thing be extract of holoside Ge by volume by the first ultrafiltration thing and distilled water.
Preferably, the ultra-filtration membrane of described ultrafiltration is for the first time 4020PS hollow-fibre membrane, and the ultra-filtration membrane of ultrafiltration is for the second time GEPW8040 poly (ether-sulfone) ultrafiltration membrane.
Preferably, ultrasonic method for breaking described in described step 1 is for adding in Ganoderma mycelium in distilled water, and ultrasonic extraction 20～40min at 50～70 ℃, obtains solidliquid mixture.
Preferably, further comprising the steps of:
Step 6, holoside Ge extract in step 5 is concentrated into 1/6 of original volume, adds ethanol to make ethanol final concentration in solution reach 70～80%, standing 5～8h, 3000rpm is centrifugal, abandon supernatant liquor, 40 ℃ of vacuum-dryings, obtain lucidum mycelium polysaccharide germanium second extract.
Preferably, concentration method is vacuum concentration.
The invention has the beneficial effects as follows,
1. in the inventive method in step 1, for prior art, do not need Ganoderma mycelium the preprocessing process such as to clean, only the solidliquid mixture after ultrasonication need to be processed to the impurity that can remove in Ganoderma mycelium and the broken thing of Ganoderma mycelium through charcoal absorption, simplified technical process, and without any need for clean-out system, reduced the application of chemical reagent, protection of the environment;
2. the employing gac of this process using decolours in water, absorption, make the broken thing of Ganoderma mycelium, insolubles in water become particle with charcoal absorption, be easy to remove, also given up by Sevag method except this step of albumen simultaneously, having abandoned a large amount of organic dissolvings such as chloroform, propyl carbinol, is the friendly type technique of a kind of green environment.
3. adopt ultrasonic technology to extract lucidum mycelium polysaccharide germanium, extraction time is short, and extraction efficiency is high, and mild condition, is a kind of technique that is appropriate to industrialized production.
4. adopt ultra-filtration membrane and hollow-fibre membrane purification of ganoderma lucidum mycelium polysaccharides germanium, orientation has been isolated current product lucidum mycelium polysaccharide germanium accurately, and this technological operation is simple, technology maturation, and obtained high purity target product, product purity reaches more than 98%.
Accompanying drawing explanation
Fig. 1 is the process flow sheet of the extracting method of lucidum mycelium polysaccharide germanium of the present invention.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, to make those skilled in the art can implement according to this with reference to specification sheets word.
Embodiment 1
As shown in Figure 1, a kind of extracting method of lucidum mycelium polysaccharide germanium, is characterized in that, comprises the following steps:
Step 1, Ganoderma mycelium is added in distilled water, ultrasonic extraction 20～40min at 50～70 ℃, obtains solidliquid mixture and obtains solidliquid mixture;
Step 2, by solidliquid mixture and activated carbon, be to mix at 100: 2 by volume, insulated and stirred 45min at 60 ℃, press filtration afterwards obtains the first extracting solution;
Step 3, described the first extracting solution is carried out to vacuum concentration obtain the first enriched material;
Step 4, acquisition the first enriched material are dissolved in ethanol and make ethanol final concentration in solution reach 60%, room temperature standing over night, and the centrifugal 20min of 3200rpm, obtains the second enriched material;
Step 5, the second enriched material obtaining is carried out to two times of ultrafiltration be continuously respectively ultrafiltration for the first time and ultrafiltration for the second time in step 4, be, by the second enriched material and distilled water, be to mix at 1: 8 to carry out ultrafiltration for the first time and obtain the first ultrafiltration thing by volume, and be to mix at 1: 8 to carry out ultrafiltration for the second time to obtain the second ultrafiltration thing be extract of holoside Ge by volume by the first ultrafiltration thing and distilled water;
The condition of ultrafiltration is for the first time: ultra-filtration membrane aperture is 0.002 μ m, and ultrafiltration flow velocity is 3L/h, and ultrafiltration pressure is 0.10MPa; The condition of ultrafiltration is for the second time: ultra-filtration membrane aperture is 0.001 μ m, ultrafiltration flow velocity is 3L/h, and ultrafiltration pressure is 0.3MPa, wherein, the ultra-filtration membrane of described ultrafiltration for the first time can be 4020PS hollow-fibre membrane, and the ultra-filtration membrane of ultrafiltration for the second time can be GEPW8040 poly (ether-sulfone) ultrafiltration membrane;
Step 6, holoside Ge extract in step 5 is concentrated into 1/6 of original volume, adds ethanol to make ethanol final concentration in solution reach 70%, standing 5h, 3000rpm is centrifugal, abandons supernatant liquor, and 40 ℃ of vacuum-dryings obtain lucidum mycelium polysaccharide germanium second extract.
Embodiment 2
As shown in Figure 1, a kind of extracting method of lucidum mycelium polysaccharide germanium, is characterized in that, comprises the following steps:
Step 1, Ganoderma mycelium is added in distilled water, ultrasonic extraction 20min at 50 ℃, obtains solidliquid mixture and obtains solidliquid mixture;
Step 2, by solidliquid mixture and activated carbon, be to mix at 100: 1 by volume, insulated and stirred 30min at 60 ℃, press filtration afterwards obtains the first extracting solution;
Step 3, described the first extracting solution is carried out to vacuum concentration obtain the first enriched material;
Step 4, acquisition the first enriched material are dissolved in ethanol and make ethanol final concentration in solution reach 70%, room temperature standing over night, and the centrifugal 20min of 3000rpm, obtains the second enriched material;
Step 5, the second enriched material obtaining is carried out to two times of ultrafiltration be continuously respectively ultrafiltration for the first time and ultrafiltration for the second time in step 4, be, by the second enriched material and distilled water, be to mix at 1: 9 to carry out ultrafiltration for the first time and obtain the first ultrafiltration thing by volume, and be to mix at 1: 9 to carry out ultrafiltration for the second time to obtain the second ultrafiltration thing be extract of holoside Ge by volume by the first ultrafiltration thing and distilled water.
The condition of ultrafiltration is for the first time: ultra-filtration membrane aperture is 0.003 μ m, and ultrafiltration flow velocity is 4L/h, and ultrafiltration pressure is 0.13MPa; The condition of ultrafiltration is for the second time: ultra-filtration membrane aperture is 0.002 μ m, ultrafiltration flow velocity is 4L/h, and ultrafiltration pressure is 0.4MPa, wherein, the ultra-filtration membrane of described ultrafiltration for the first time can be 4020PS hollow-fibre membrane, and the ultra-filtration membrane of ultrafiltration for the second time can be GEPW8040 poly (ether-sulfone) ultrafiltration membrane;
Step 6, holoside Ge extract in step 5 is concentrated into 1/6 of original volume, adds ethanol to make ethanol final concentration in solution reach 75%, standing 6.5h, 3000rpm is centrifugal, abandons supernatant liquor, and 40 ℃ of vacuum-dryings obtain lucidum mycelium polysaccharide germanium second extract.
Embodiment 3
As shown in Figure 1, a kind of extracting method of lucidum mycelium polysaccharide germanium, is characterized in that, comprises the following steps:
Step 1, Ganoderma mycelium is added in distilled water, ultrasonic extraction 40min at 70 ℃, obtains solidliquid mixture and obtains solidliquid mixture;
Step 2, by solidliquid mixture and activated carbon, be to mix at 100: 4 by volume, insulated and stirred 60min at 70 ℃, press filtration afterwards obtains the first extracting solution; Step 3, described the first extracting solution is carried out to vacuum concentration obtain the first enriched material;
Step 4, acquisition the first enriched material are dissolved in ethanol and make ethanol final concentration in solution reach 65%, room temperature standing over night, and the centrifugal 20min of 3400rpm, obtains the second enriched material;
Step 5, the second enriched material obtaining is carried out to two times of ultrafiltration be continuously respectively ultrafiltration for the first time and ultrafiltration for the second time in step 4, be, by the second enriched material and distilled water, be to mix at 1: 10 to carry out ultrafiltration for the first time and obtain the first ultrafiltration thing by volume, and be to mix at 1: 10 to carry out ultrafiltration for the second time to obtain the second ultrafiltration thing be extract of holoside Ge by volume by the first ultrafiltration thing and distilled water.
The condition of ultrafiltration is for the first time: ultra-filtration membrane aperture is 0.004 μ m, and ultrafiltration flow velocity is 5L/h, and ultrafiltration pressure is 0.15MPa; The condition of ultrafiltration is for the second time: ultra-filtration membrane aperture is 0.003 μ m, and ultrafiltration flow velocity is 5L/h, and ultrafiltration pressure is 0.5MPa.
The ultra-filtration membrane of described ultrafiltration is for the first time 4020PS hollow-fibre membrane, and the ultra-filtration membrane of ultrafiltration is for the second time GEPW8040 poly (ether-sulfone) ultrafiltration membrane.
Step 6, holoside Ge extract in step 5 is concentrated into 1/6 of original volume, adds ethanol to make ethanol final concentration in solution reach 80%, standing 8h, 3000rpm is centrifugal, abandons supernatant liquor, and 40 ℃ of vacuum-dryings obtain lucidum mycelium polysaccharide germanium second extract.
Application the inventive method is carried out the extraction of holoside Ge under laboratory condition, and purity and the content of the holoside Ge of Measurement accuracy acquisition:
Embodiment 4
To in 10 kilograms of Ganoderma myceliums, add in 60L distilled water, ultrasonic extraction 20min at 60 ℃, then insulation, adds activated carbon in extractor, insulated and stirred 30min, press filtration obtains clear liquid Ganoderma mycelium extracting solution.Ganoderma mycelium extracting solution obtained in the previous step is concentrated, then add 95% ethanol to make final concentration reach 70%, room temperature standing over night, the centrifugal 20min of 3000rpm, obtains Crude polysaccharides germanium crude product.Crude polysaccharides germanium is dissolved in distilled water, first washing equipment, after determining that pipeline and container are clean, raw material tank packs feed liquid into, by the ultrafiltration apparatus that is assembly with 4020PS hollow-fibre membrane, with 3.0L/h flow amount, pressure is that the flow velocity of 0.10～0.15 MPa concentrates, molecular weight cut-off more than 30,000, sees through liquid and gets single step purification ready.The liquid that sees through obtained in the previous step is packed in hopper, the ultrafiltration apparatus that is assembly with GEPW8040 ultra-filtration membrane, pressure is 0.3 MPa, with the flow velocity of 3.0L/h, concentrates, molecular weight cut-off is more than 10,000, through liquid be recovered in storage tank, reclaim stand-by.Concentrated solution is got single step purification ready.Above-mentioned holoside Ge solution is added and is concentrated into 1/6 of original volume, add 95% ethanol to make final concentration reach 70%, the standing 5h of precipitation polysaccharide, 3000rpm is centrifugal, abandons supernatant liquor, and vacuum-drying obtains lucidum mycelium polysaccharide germanium fine work, and purity is 98.3%.
Embodiment 5
To in 10 kilograms of Ganoderma myceliums, add in 60L distilled water, ultrasonic extraction 25min at 70 ℃, then insulation, adds activated carbon in extractor, insulated and stirred 30min, press filtration obtains clear liquid Ganoderma mycelium extracting solution.Ganoderma mycelium extracting solution obtained in the previous step is concentrated, then add 95% ethanol to make final concentration reach 70%, room temperature standing over night, the centrifugal 20min of 3000rpm, obtains Crude polysaccharides germanium crude product.Crude polysaccharides germanium is dissolved in distilled water, first washing equipment, after determining that pipeline and container are clean, raw material tank packs feed liquid into, by the ultrafiltration apparatus that is assembly with 4020PS hollow-fibre membrane, with 4.0L/h flow amount, pressure is that the flow velocity of 0.10～0.15 MPa concentrates, molecular weight cut-off more than 30,000, sees through liquid and gets single step purification ready.The liquid that sees through obtained in the previous step is packed in hopper, the ultrafiltration apparatus that is assembly with GEPW8040 ultra-filtration membrane, pressure is 0.5 MPa, with the flow velocity of 4.0L/h, concentrates, molecular weight cut-off is more than 10,000, through liquid be recovered in storage tank, reclaim stand-by.Concentrated solution is got single step purification ready.Above-mentioned holoside Ge solution is added and is concentrated into 1/6 of original volume, add 95% ethanol to make final concentration reach 75%, the standing 5.0h of precipitation polysaccharide, 3000rpm is centrifugal, abandons supernatant liquor, and vacuum-drying obtains lucidum mycelium polysaccharide germanium fine work, and purity is 98.8%.
Although embodiment of the present invention are open as above, but it is not restricted to listed utilization in specification sheets and embodiment, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other modification, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details and shown here embodiment.
Claims (10)
1. an extracting method for lucidum mycelium polysaccharide germanium, is characterized in that, comprises the following steps:
Step 1, Ganoderma mycelium is carried out to ultrasonic disruption process to obtain solidliquid mixture;
Step 2, by solidliquid mixture and activated carbon, be 100: 1～4 to mix by volume, insulated and stirred 30～60min at 50～70 ℃, press filtration afterwards obtains the first extracting solution;
Step 3, described the first extracting solution is carried out to vacuum concentration obtain the first enriched material;
Step 4, acquisition the first enriched material are dissolved in ethanol and make ethanol final concentration in solution reach 60～70%, room temperature standing over night, and the centrifugal 20min of 3000-3400rpm, obtains the second enriched material;
Step 5, the second enriched material obtaining is carried out to two times of ultrafiltration be continuously respectively ultrafiltration for the first time and ultrafiltration for the second time in step 4, obtain extract of holoside Ge.
2. the extracting method of lucidum mycelium polysaccharide germanium as claimed in claim 1, is characterized in that, in step 2, solidliquid mixture and activated carbon are 100: 1～2 to mix by volume, insulated and stirred 40～50min at 60～65 ℃, and press filtration afterwards obtains the first extracting solution.
3. the extracting method of lucidum mycelium polysaccharide germanium as claimed in claim 2, it is characterized in that, in described step 4, obtain the first enriched material and be dissolved in ethanol and make ethanol final concentration in solution reach 70%, room temperature standing over night, the centrifugal 20min of 3000rpm, obtains the second enriched material.
4. the extracting method of lucidum mycelium polysaccharide germanium as claimed in claim 3, is characterized in that, the condition of ultrafiltration is for the first time: ultra-filtration membrane aperture is 0.002～0.004 μ m, and ultrafiltration flow velocity is 3～5L/h, and ultrafiltration pressure is 0.10～0.15MPa; The condition of ultrafiltration is for the second time: ultra-filtration membrane aperture is 0.001～0.003 μ m, and ultrafiltration flow velocity is 3～5L/h, and ultrafiltration pressure is 0.3～0.5MPa.
5. the extracting method of lucidum mycelium polysaccharide germanium as claimed in claim 4, it is characterized in that, the condition of described ultrafiltration is for the first time: adopt 4020PS hollow-fibre membrane, ultra-filtration membrane aperture is 0.002 μ m, ultrafiltration flow velocity is 3～5L/h, and ultrafiltration pressure is 0.10～0.15MPa; The condition of ultrafiltration is for the second time: adopt GEPW8040F poly (ether-sulfone) ultrafiltration membrane, ultra-filtration membrane aperture is 0.001 μ m, and ultrafiltration flow velocity is 3～5L/h, and ultrafiltration pressure is 0.3～0.5MPa.
6. the extracting method of lucidum mycelium polysaccharide germanium as claimed in claim 5, it is characterized in that, in described step 5, also comprise by the second enriched material and distilled water being 1: 8～10 to mix and carry out ultrafiltration for the first time and obtain the first ultrafiltration thing by volume, and be 1: 8～10 to mix and carry out ultrafiltration for the second time to obtain the second ultrafiltration thing be extract of holoside Ge by volume by the first ultrafiltration thing and distilled water.
7. the extracting method of lucidum mycelium polysaccharide germanium as claimed in claim 6, is characterized in that, the ultra-filtration membrane of described ultrafiltration is for the first time 4020PS hollow-fibre membrane, and the ultra-filtration membrane of ultrafiltration is for the second time GEPW8040 poly (ether-sulfone) ultrafiltration membrane.
8. the extracting method of lucidum mycelium polysaccharide germanium as claimed in claim 7, it is characterized in that, ultrasonic method for breaking described in described step 1 is for adding in Ganoderma mycelium in distilled water, and ultrasonic extraction 20～40min at 50～70 ℃, obtains solidliquid mixture.
9. the extracting method of lucidum mycelium polysaccharide germanium as claimed in claim 8, is characterized in that, further comprising the steps of:
Step 6, holoside Ge extract in step 5 is concentrated into 1/6 of original volume, adds ethanol to make ethanol final concentration in solution reach 70～80%, standing 5～8h, 3000rpm is centrifugal, abandon supernatant liquor, 40 ℃ of vacuum-dryings, obtain lucidum mycelium polysaccharide germanium second extract.
10. the extracting method of lucidum mycelium polysaccharide germanium as claimed in claim 9, is characterized in that, concentration method is vacuum concentration.
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| CN1120543A (en) * | 1995-04-26 | 1996-04-17 | 中国药科大学 | Extraction, separation and purification of "jingu" mushroom holoside Ge and its inhibition effect on reverse-transcriptase of Aids virus |
| CN1513879A (en) * | 2003-08-14 | 2004-07-21 | 中国药科大学 | Germanium enriced gold needle mushroom polysaccharide and its making metehod and use |
| CN1687438A (en) * | 2005-05-18 | 2005-10-26 | 刘年生 | Method for extracting ganoderma lucidum amylose in high germanium and ganoderma lucidum acid |
| CN102091278A (en) * | 2010-09-08 | 2011-06-15 | 成进学 | Selenium- and germanium-enriched lucid ganoderma mycelium snake nano metallothionein capsule |
| CN102977222A (en) * | 2012-12-20 | 2013-03-20 | 西藏金稞集团有限责任公司 | Method for extracting ganoderma lucidum mycelia polysaccharide germanium |
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