CN104829742A - Phellinus linteus polysaccharide separation and purification method - Google Patents
Phellinus linteus polysaccharide separation and purification method Download PDFInfo
- Publication number
- CN104829742A CN104829742A CN201510244767.3A CN201510244767A CN104829742A CN 104829742 A CN104829742 A CN 104829742A CN 201510244767 A CN201510244767 A CN 201510244767A CN 104829742 A CN104829742 A CN 104829742A
- Authority
- CN
- China
- Prior art keywords
- phellinus
- granulose
- purification
- separation
- polysaccharide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention discloses a Phellinus linteus polysaccharide separation and purification method which is characterized by mainly comprising the following steps: preparing a Phellinus linteus polysaccharide component by an ammonium oxalate extraction-ethanol precipitation process, carrying out anion exchange chromatography and gel filtration chromatography to separate and purify the polysaccharide, and carrying out concentration, dialysis, vacuum freeze drying and the like on the product to obtain the Phellinus linteus polysaccharide pure product. The method does not influence the chemical structure of the natural polysaccharide, and can keep the original bioactivity of the natural polysaccharide. The method has the advantages of low requirements for equipment and environment, simple process and low cost, is flexible and practical, and is beneficial to popularization, development and application.
Description
Technical field
The present invention relates to the method for a kind of applied bioengineering technology separation and purification Phellinus granulose from phellinus igniarius mycelium.
Background technology
Phellinus bacterium (
phellinus linteus) belong to Basidiomycotina, polyporaceae, wood layer hole strain (
phellinus), be a kind of medicinal fungi of preciousness, have the laudatory title of " forest gold ".Research finds; Phellinus has significant antitumor action; to the restraining effect of S180, cancer of the stomach, comparatively the medicinal fungi such as glossy ganoderma, Brazilian mushroom is also strong; it is the efficient medicinal fungi that ranked first in current internationally recognized biology field for cancer; have the potentiality of exploitation natural antitumor biological products, wherein polysaccharide is main active ingredient.However; the antitumor action of domestic and international research Phellinus polysaccharide also main with aqueous extract or water extraction polysaccharide for research object; for the polysaccharide that other Extraction medium are prepared as acid solution, alkali lye, research is also less both at home and abroad at present, limits deep development and the application of Phellinus bacterium active polysaccharide.
Through retrieval, the separation purification method carrying Phellinus granulose about acid at present have not been reported.
Summary of the invention
The object of the present invention is to provide the method for a kind of Phellinus granulose separation and purification, do not affect the chemical structure of natural polysaccharide, retain its biological activity, the enable medicinal raw material as injection, capsule etc., also can be used as the batching of healthcare products, functional food.
In order to solve above technical problem, the present invention is extracted by ammonium oxalate solution from the phellinus igniarius mycelium of liquid fermentation and culture bioactive Crude polysaccharides, removing protein, dialysis, column chromatography purification and lyophilize, and concrete technical scheme is as follows:
A method for Phellinus granulose separation and purification, is characterized in that comprising the following steps:
Step one, degreasing: by phellinus igniarius mycelium pulverize, with organic solvent in a usual manner degreasing obtain the phellinus igniarius mycelium after degreasing;
Step 2, acid is carried: the phellinus igniarius mycelium after described degreasing is added the ammonium oxalate solution that mass concentration is 1%, stirs; At 95 DEG C, refluxing extraction is centrifugal again after 8 hours again, and concentrated, the mass concentration adding 4 times of volumes in last concentrated extracting solution is the ethanol of 95%, 4 DEG C of refrigerator hold over night, sourly must carry Phellinus bacterium Crude polysaccharides after centrifugal collecting precipitation; Phellinus igniarius mycelium after described degreasing and the ratio of ammonium oxalate solution are 1:10 g/mL;
Step 3, removing protein: described acid is carried Phellinus bacterium Crude polysaccharides and be dissolved in distilled water, Sevag reagent method removes wherein albumen, obtains the polysaccharide solution removing albumen;
Step 4, dialysis: the polysaccharide solution of described removal albumen is concentrated, centrifugal, discard insolubles, supernatant liquor, supernatant liquor to be dialysed in flowing water 48 h then 48 h that dialyse in distilled water with dialysis tubing, the small molecular weight impurities such as removing oligosaccharides, inorganic salt, pigment, collect the extracting solution obtaining Phellinus granulose;
Step 5; column chromatography purification: the extracting solution of described Phellinus granulose is concentrated; through DEAE-Sephadex A-25 Fast Flow ion-exchange and Sephacryl S-400 HR gel filtration chromatography; collect liquid phend-sulphuric acid to detect, merge the collection liquid at polysaccharide peak, concentrated; dialysis; centrifugal, vacuum lyophilization, obtains the pure Phellinus granulose of chromatography.
In described step one: organic solvent is sherwood oil, boiling range 30 ~ 60 DEG C; Described usual manner is 35 DEG C of backflow 6 h in Soxhlet extractor, naturally dries.
In described step 2: the massfraction of ammonium oxalate solution is 1%; Centrifugal speed is 5000 rpm, and centrifugation time is 20 min.
In described step 3: Sevag reagent is that chloroform and propyl carbinol mix gained according to 5:1 volume ratio.
In described step 5: the chromatography column size of DEAE-Sephadex A-25 Fast Flow is 2.6 cm × 60 cm, and applied sample amount is 10 mL, and elutriant is the NaCl of 0 ~ 0.5 mol/L, and flow velocity is 2.0 mL/min.
In described step 5: Sephacryl S-400HR gel permeation chromatography post size is 1.5 cm × 100 cm, and applied sample amount is 5.0 mL, and elutriant is deionized water, and flow velocity is 2.0 mL/min.
In described step 5: dialyse as distill water dialysis 48 h, deionized water is dialysed 24 h; Centrifugal speed is 8000 rpm, and centrifugation time is 30 min.
The present invention has beneficial effect.The present invention, by setting up the separation purifying technique of Phellinus granulose, obtains the polysaccharide of homogeneous molecular weight, and composition is glucose and seminose, has immunomodulatory, anti-oxidant and anti-tumor activity.To further its chemical structure of research with illustrate equal important in inhibiting such as its pharmacologically active mechanism, structure activity relationship etc., and provide valuable theoretical foundation for the research and development of Phellinus natural antitumor biological products or functional food.
Accompanying drawing explanation
Fig. 1 is that polysaccharide of the present invention goes out 2 components through DEAE-Sepharose Fast Flow column chromatography for separation.
Fig. 2 is the Sephacryl S-400HR gel-filtration collection of illustrative plates at the 1st peak of the present invention.
Fig. 3 is the 1st gpc chromatogram of peak after gel filtration chromatography of the present invention.
Embodiment
Below in conjunction with the drawings and specific embodiments, technical scheme of the present invention is described in further detail.
embodiment 1
1, degreasing: phellinus igniarius mycelium is pulverized, degreasing cotton wraps up, and is placed in 500 mL Soxhlet extractors, adds 200 ~ 300 mL sherwood oils (boiling range: 30 ~ 60 DEG C), and 35 DEG C of backflow 6 h, repeat degreasing 1 time, naturally dry.
2, acid is carried: phellinus igniarius mycelium 100 g after the degreasing of step 1 gained is by 1:10(g/mL) solid-liquid ratio to add massfraction be 1% ammonium oxalate solution 1000 mL, be placed in 2000 mL round-bottomed flasks, stir, 95 DEG C of refluxing extraction 8 h.Mixture 300 mL plastic centrifuge tubes, 5000 rpm, 20 min are centrifugal, and supernatant liquor merges 45 DEG C of rotatory evaporator reduction vaporizations; be concentrated into 1.5 times of original volume, add 95% ethanol of 4 times of volumes, 4 DEG C of refrigerator hold over night; centrifugal collecting precipitation, obtains and sourly carries Phellinus bacterium Crude polysaccharides.Repeat extraction 1 time.
3, removing protein: the acid of step 2 gained is carried Phellinus bacterium Crude polysaccharides and is dissolved in distilled water; chloroform and propyl carbinol are mixed with Sevag reagent according to 5:1 volume ratio; Sevag reagent is added by polysaccharide solution 1:5 volume; mixture is in water-bath vibration pot; under room temperature, 1 h is stirred in jolting; 5000 rpm, 20 min are centrifugal, except Deproteinization.Repetition removing protein like this 8 ~ 10 times.
4, dialyse: the polysaccharide solution of the removing protein of step 3 gained concentrates, centrifugal, abandons insolubles; supernatant liquor, supernatant liquor to be dialysed in flowing water 48 h then 48 h that dialyse in distilled water with dialysis tubing; the small molecular weight impurities such as removing oligosaccharides, inorganic salt, pigment, collect the extracting solution of Phellinus granulose.
5, column chromatography purification: by the liquid concentration after dialysing through step 4, through DEAE-Sephadex A-25 Fast Flow ion-exchange and Sephacryl S-400HR gel filtration chromatography.
1) ion-exchange chromatography (DEAE-Sephadex A-25 Fast Flow) is separated classification: deionized water balance DEAE-Sephadex A-25 Fast Flow(2.6 cm × 60 cm), applied sample amount 10 mL, with the NaCl gradient elution of 0-0.5 mol/L, flow velocity 2.0 mL/min, phend-sulphuric acid tracing detection polysaccharide goes out peak, draws elution curve.Isolate 2 components, result as shown in Figure 1.Concentrated after collecting, dialysis and lyophilize, the polysaccharide recovery of two components is respectively 63.61% and 30.36%, total yield 93.97%, the 1st component for needed for.
2) gel filtration chromatography post (Sephacryl S-400 HR) purifying: the 1st fraction polysaccharide is dissolved in deionized water, upper Sephacryl S-400HR gel permeation chromatography post (1.5 cm × 100 cm), applied sample amount 5.0 mL, with deionized water wash-out, flow velocity 2.0 mL/min, phend-sulphuric acid tracing detection polysaccharide goes out peak, and draw elution curve, result as shown in Figure 2.Collect simple spike, 4 DEG C of preservations.
6, lyophilize: after the polysaccharide fraction collected is concentrated; distill water dialysis 48 h; deionized water is dialysed 24 h, and 45 DEG C of rotatory evaporator reduction vaporizations are to proper volume, and 8000 rpm, 30 min are centrifugal; vacuum lyophilization; obtain the Phellinus granulose sterling of white flock, polysaccharide recovery reaches 89.6%, and it is 99.0% that phend-sulphuric acid records neutral sugar content; detect purity through gel permeation chromatography (GPC) and reach more than 90%, result as shown in Figure 3.And to record weight-average molecular weight be 10.6 kDa.
Claims (7)
1. a method for Phellinus granulose separation and purification, is characterized in that comprising the following steps:
Step one, degreasing: by phellinus igniarius mycelium pulverize, with organic solvent in a usual manner degreasing obtain the phellinus igniarius mycelium after degreasing;
Step 2, acid is carried: the phellinus igniarius mycelium after described degreasing is added the ammonium oxalate solution that mass concentration is 1%, stirs; At 95 DEG C, refluxing extraction is centrifugal again after 8 hours again, and concentrated, the mass concentration adding 4 times of volumes in last concentrated extracting solution is the ethanol of 95%, 4 DEG C of refrigerator hold over night, sourly must carry Phellinus bacterium Crude polysaccharides after centrifugal collecting precipitation; Phellinus igniarius mycelium after described degreasing and the ratio of ammonium oxalate solution are 1:10 g/mL;
Step 3, removing protein: described acid is carried Phellinus bacterium Crude polysaccharides and be dissolved in distilled water, Sevag reagent method removes wherein albumen, obtains the polysaccharide solution removing albumen;
Step 4, dialysis: the polysaccharide solution of described removal albumen is concentrated, centrifugal, discard insolubles, supernatant liquor, supernatant liquor to be dialysed in flowing water 48 h then 48 h that dialyse in distilled water with dialysis tubing, the small molecular weight impurities such as removing oligosaccharides, inorganic salt, pigment, collect the extracting solution obtaining Phellinus granulose;
Step 5; column chromatography purification: the extracting solution of described Phellinus granulose is concentrated; through DEAE-Sephadex A-25 Fast Flow ion-exchange and Sephacryl S-400 HR gel filtration chromatography; collect liquid phend-sulphuric acid to detect, merge the collection liquid at polysaccharide peak, concentrated; dialysis; centrifugal, vacuum lyophilization, obtains the pure Phellinus granulose of chromatography.
2. the method for a kind of Phellinus granulose according to claim 1 separation and purification, is characterized in that in described step one: organic solvent is sherwood oil, boiling range 30 ~ 60 DEG C; Described usual manner is 35 DEG C of backflow 6 h in Soxhlet extractor, naturally dries.
3. the method for a kind of Phellinus granulose according to claim 1 separation and purification, is characterized in that in described step 2: the massfraction of ammonium oxalate solution is 1%; Centrifugal speed is 5000 rpm, and centrifugation time is 20 min.
4. the method for a kind of Phellinus granulose according to claim 1 separation and purification, is characterized in that in described step 3: Sevag reagent is that chloroform and propyl carbinol mix gained according to 5:1 volume ratio.
5. the method for a kind of Phellinus granulose according to claim 1 separation and purification; it is characterized in that in described step 5: the chromatography column size of DEAE-Sephadex A-25 Fast Flow is 2.6 cm × 60 cm; applied sample amount is 10 mL; elutriant is the NaCl of 0 ~ 0.5 mol/L, and flow velocity is 2.0 mL/min.
6. the method for a kind of Phellinus granulose according to claim 1 separation and purification; it is characterized in that in described step 5: Sephacryl S-400HR gel permeation chromatography post size is 1.5 cm × 100 cm; applied sample amount is 5.0 mL, and elutriant is deionized water, and flow velocity is 2.0 mL/min.
7. the method for a kind of Phellinus granulose according to claim 1 separation and purification, is characterized in that in described step 5: dialyse as distill water dialysis 48 h, and deionized water is dialysed 24 h; Centrifugal speed is 8000 rpm, and centrifugation time is 30 min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510244767.3A CN104829742A (en) | 2015-05-14 | 2015-05-14 | Phellinus linteus polysaccharide separation and purification method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510244767.3A CN104829742A (en) | 2015-05-14 | 2015-05-14 | Phellinus linteus polysaccharide separation and purification method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104829742A true CN104829742A (en) | 2015-08-12 |
Family
ID=53807967
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510244767.3A Pending CN104829742A (en) | 2015-05-14 | 2015-05-14 | Phellinus linteus polysaccharide separation and purification method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104829742A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105461819A (en) * | 2015-12-08 | 2016-04-06 | 西藏天虹科技股份有限责任公司 | Agaricus blazei Murill polysaccharides and extraction method thereof |
| CN105968223A (en) * | 2016-07-05 | 2016-09-28 | 中国农业科学院作物科学研究所 | Preparation method of mung bean polysaccharide with immunoregulatory activity |
| CN106576780A (en) * | 2016-11-30 | 2017-04-26 | 广州市瑞硒康生物科技有限公司 | Cultivating method for selenium-rich cardamine hupingshanesis and extracting method for selenium-rich cardamine hupingshanesis |
| CN110483661A (en) * | 2019-08-21 | 2019-11-22 | 江苏江南生物科技有限公司 | A kind of straw mushroom polysaccharide and its preparation method and application |
| CN111019008A (en) * | 2019-12-13 | 2020-04-17 | 浙江省农业科学院 | Anti-inflammatory activity phellinus igniarius polysaccharide SHP and preparation method thereof |
| CN111978421A (en) * | 2020-08-21 | 2020-11-24 | 浙江省林业科学研究院 | Phellinus igniarius polysaccharide and preparation and application thereof |
| CN113398153A (en) * | 2021-06-22 | 2021-09-17 | 安徽利民生物科技股份有限公司 | Method for utilizing phellinus igniarius mycelium |
| CN114292344A (en) * | 2022-01-17 | 2022-04-08 | 海南华创槟榔研究院 | Method for purifying neutral sugar in betel nut and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS4932931A (en) * | 1972-07-27 | 1974-03-26 | ||
| CN101323648A (en) * | 2008-07-29 | 2008-12-17 | 上海璞诚生物科技有限公司 | Extraction method and and purification method of Sanghuang mushroom polysaccharide |
| CN103319618A (en) * | 2013-06-21 | 2013-09-25 | 上海市农业科学院 | Preparation method of active polysaccharide of male agaric mycelium |
-
2015
- 2015-05-14 CN CN201510244767.3A patent/CN104829742A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS4932931A (en) * | 1972-07-27 | 1974-03-26 | ||
| CN101323648A (en) * | 2008-07-29 | 2008-12-17 | 上海璞诚生物科技有限公司 | Extraction method and and purification method of Sanghuang mushroom polysaccharide |
| CN103319618A (en) * | 2013-06-21 | 2013-09-25 | 上海市农业科学院 | Preparation method of active polysaccharide of male agaric mycelium |
Non-Patent Citations (3)
| Title |
|---|
| WANG ZHENBIN等: "effect of extraction media on preliminary characterizations and antioxidant activities of Phellinus linteus polysaccharises", 《CARBOHYDRATE POLYMERS》 * |
| 徐双阳: "桑黄多糖提取、分离纯化及理化性质研究", 《中国优秀博硕士学位论文全文数据库（硕士）工程科技Ⅰ辑》 * |
| 葛青: "桑黄子实体多糖的分离纯化、结构鉴定及结构修饰研究", 《中国博士学位论文全文数据库工程科技Ⅰ辑》 * |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105461819A (en) * | 2015-12-08 | 2016-04-06 | 西藏天虹科技股份有限责任公司 | Agaricus blazei Murill polysaccharides and extraction method thereof |
| CN105968223A (en) * | 2016-07-05 | 2016-09-28 | 中国农业科学院作物科学研究所 | Preparation method of mung bean polysaccharide with immunoregulatory activity |
| CN106576780A (en) * | 2016-11-30 | 2017-04-26 | 广州市瑞硒康生物科技有限公司 | Cultivating method for selenium-rich cardamine hupingshanesis and extracting method for selenium-rich cardamine hupingshanesis |
| CN110483661A (en) * | 2019-08-21 | 2019-11-22 | 江苏江南生物科技有限公司 | A kind of straw mushroom polysaccharide and its preparation method and application |
| CN110483661B (en) * | 2019-08-21 | 2021-09-24 | 江苏江南生物科技有限公司 | Straw mushroom polysaccharide and preparation method and application thereof |
| CN111019008A (en) * | 2019-12-13 | 2020-04-17 | 浙江省农业科学院 | Anti-inflammatory activity phellinus igniarius polysaccharide SHP and preparation method thereof |
| CN111019008B (en) * | 2019-12-13 | 2022-04-05 | 浙江省农业科学院 | Anti-inflammatory activity phellinus igniarius polysaccharide SHP and preparation method thereof |
| CN111978421A (en) * | 2020-08-21 | 2020-11-24 | 浙江省林业科学研究院 | Phellinus igniarius polysaccharide and preparation and application thereof |
| CN113398153A (en) * | 2021-06-22 | 2021-09-17 | 安徽利民生物科技股份有限公司 | Method for utilizing phellinus igniarius mycelium |
| CN113398153B (en) * | 2021-06-22 | 2022-07-08 | 安徽利民生物科技股份有限公司 | Method for utilizing phellinus igniarius mycelium |
| CN114292344A (en) * | 2022-01-17 | 2022-04-08 | 海南华创槟榔研究院 | Method for purifying neutral sugar in betel nut and application thereof |
| CN114292344B (en) * | 2022-01-17 | 2022-11-15 | 海南华创槟榔研究院 | Method for purifying neutral sugar in betel nut and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104829742A (en) | Phellinus linteus polysaccharide separation and purification method | |
| CN104861082B (en) | Method for separating polysaccharide and protein by using choline ionic liquid two-phase aqueous system | |
| CN107602720B (en) | Method for separating cynomorium songaricum polysaccharide by eutectic solvent/salt aqueous two-phase extraction | |
| CN101416721B (en) | Method for extracting a great variety of biological active ingredients from dry powder of pasania fungus | |
| CN102936292B (en) | Preparation method of lycium barbarum polysaccharide having high antioxidant activity | |
| CN101899122B (en) | Aqueous two-phase system extraction method for ganodenic acid and ganoderma lucidum polysaccharide | |
| CN104926937A (en) | Method for extracting hirudin from leech saliva | |
| CN101353382B (en) | Extraction method of antioxidative active Cordyceps sinensis polysaccharide | |
| CN104910289A (en) | Method for continuously preparing phellinus igniarius mycelium polysaccharide | |
| CN100447250C (en) | Separation and refining process for rice bran polysaccharide | |
| CN105603029A (en) | Extraction method of active walnut peptides | |
| CN103467618B (en) | A kind of method of Cordyceps mycelium separation of polysaccharides purifying | |
| CN101020721A (en) | Process of preparing high purity astragalus polysaccharide | |
| CN102775466A (en) | Preparation method of selenium-containing protein in selenium-enriched yeast | |
| CN101445565A (en) | Separation and purification method for Dunaliella salina polysaccharide | |
| CN101003578A (en) | Method for separating and purifying paecilomyces cicadae polysaccharide | |
| CN103275237B (en) | Preparation method and application of eggplant branch polysaccharide | |
| CN104292355B (en) | A kind of extracting method of platycodon root polysaccharide | |
| CN104277135A (en) | Pleurotus tuber-regium polysaccharide and extraction method thereof | |
| CN103554285A (en) | Extraction method of chanterelle mycelium polysaccharide | |
| CN103694367B (en) | A kind of extraction and application method with anti-oxidant activity clam polysaccharide | |
| CN102653567A (en) | Extracting process of cordyceps sinensis mycelium polysaccharide of composite preparation for preventing tumor | |
| CN102617748A (en) | Method for purifying pullulan polysaccharide | |
| CN105294878A (en) | Preparation method of mulberry twig antineoplastic activity polysaccharide RMPW-1 | |
| CN103265644B (en) | Preparation method of white muscardine silkworm anticancer-activity polysaccharide BBPW-2 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150812 |