CN104193715A - Method for extracting alpinetin from curcuma aromatica - Google Patents
Method for extracting alpinetin from curcuma aromatica Download PDFInfo
- Publication number
- CN104193715A CN104193715A CN201410450660.XA CN201410450660A CN104193715A CN 104193715 A CN104193715 A CN 104193715A CN 201410450660 A CN201410450660 A CN 201410450660A CN 104193715 A CN104193715 A CN 104193715A
- Authority
- CN
- China
- Prior art keywords
- alpinetin
- extracting
- ethanolic soln
- root tuber
- aromatic turmeric
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 0 COc1cc(*)c(C(CC(c2ccccc2)O2)=O)c2c1 Chemical compound COc1cc(*)c(C(CC(c2ccccc2)O2)=O)c2c1 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/22—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4
- C07D311/26—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3
- C07D311/28—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3 with aromatic rings attached in position 2 only
- C07D311/32—2,3-Dihydro derivatives, e.g. flavanones
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/22—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4
- C07D311/26—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3
- C07D311/40—Separation, e.g. from natural material; Purification
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a method for extracting alpinetin from curcuma aromatica. The method comprises the following steps: pulverizing curcuma aromatica raw material, adding an equal volume of water and bio-enzyme, carrying out heat-preserved enzymolysis for 3-5 hours; then adding saturated aqueous lime solution, carrying out ultrasonic extraction for 2-3 times; adjusting the pH value of the extractive solution to 5-6, adding to macroporous resin for adsorbing, carrying out gradient elution with an ethanol solution to obtain eluate, concentrating the eluate, standing for crystallizing, and dissolving crystals with alkaline water; adding an appropriate amount of ethanol, filtering, adjusting the pH value of the filtrate to 1-3, standing for crystallizing, and then crystallizing the crystalls again with an ethanol solution and drying to obtain alpinetin. By virtue of the method for extracting alpinetin from curcuma aromatica, the process is simple and easily operated, the yield of the product is high and the industrial production is easily achieved.
Description
Technical field
The invention belongs to biological technical field, particularly relate to a kind of method of extracting Alpinetin from root tuber of aromatic turmeric.
Background technology
Alpinetin (Alpinetin) chemical name (S)-7-hydroxy-5-methyl oxygen base flavones, molecular formula C
16h
14o
4; Molecular weight 270.27, molecular structural formula:
The effects such as that Alpinia japonica have is antibacterial, anti-oxidant, anticancer, antithrombotic, hypotensive, reducing blood-fat, hypoglycemic, antiemetic, analgesia.Root tuber of aromatic turmeric is zingiberaceous plant, with root, is used as medicine, and taste is pungent, bitter, cold in nature, has the use of regulating qi to disperse stagnation, heart fire-clearing upset-relieving, promoting the function of the gallbladder to alleviate jaundice, promoting blood circulation and stopping pain, promoting qi circulation and removing obstruction in the collateral, the cool blood that clears away heart-fire.Modern study finds that root tuber of aromatic turmeric piece root is containing volatile oil, curcumene, Alpinetin, demethoxycurcumin, bisdemethoxycurcumin, turmerone and aryl turmerone.
By searching document, the method that not yet has industrialization to extract Alpinetin at present from root tuber of aromatic turmeric discloses.
Summary of the invention
The present invention will address the deficiencies of the prior art and deficiency, and a kind of industrial method that extracts Alpinetin from root tuber of aromatic turmeric is provided.
The present invention realizes by the following technical solutions:
A method of extracting Alpinetin from root tuber of aromatic turmeric, is characterized in that: get root tuber of aromatic turmeric raw material pulverizing, add equal-volume water and biological enzyme, insulation enzymolysis 3-5 hour, then add saturated limewater solution supersound extraction 2-3 time, after extracting solution adjusting ph5-6, add in macroporous resin and adsorb, ethanolic soln gradient elution, the concentrated placement of elutriant crystallization, crystallisate dissolves and adds appropriate ethanol with buck, after filtration, regulates ph1-3 to place crystallization, crystallisate is used ethanolic soln crystallization again, is drying to obtain.
The optional cellulase of described enzyme, polygalacturonase and amylase, the 0.1-0.3% that consumption is amount of solid.
Described macroporous resin is a kind of in AB-8, HZ816, ADS-21 and NK-9.
Described ethanolic soln gradient elution is: 3-5 doubly measures 20-50% ethanolic soln wash-out impurity, more doubly measures 60-70% ethanolic soln wash-out effective constituent with 5-10.
Adopt the present invention from root tuber of aromatic turmeric, to extract Alpinetin, utilize biological enzyme enzymolysis to improve yield, technological operation is simple, is easy to suitability for industrialized production.
Below in conjunction with embodiment, further illustrate the present invention, but the scope of protection of present invention is not limited to the following example.
Embodiment
Embodiment 1:
Getting root tuber of aromatic turmeric raw material 10kg pulverizes, add 10L water and 20g polygalacturonase to mix thoroughly, be heated to 60 ℃, insulation enzymolysis 3 hours, then add 80L saturated limewater solution supersound extraction 30 minutes, extract 2 times, after extracting solution adjusting ph5, add in AB-8 macroporous resin and adsorb, with 4 times of amount 30% ethanolic soln wash-out impurity, again by 8 times of amount 70% ethanolic soln wash-out effective constituents, the concentrated placement of elutriant crystallization, after crystallisate dissolves with 0.1% aqueous sodium hydroxide solution, add 2 times of amount 99% ethanolic solns, after filtration, regulate ph1 to place crystallization, crystallisate is used 80% ethanolic soln crystallization again, be dried to obtain Alpinetin 13g, content 98.2%.
Embodiment 2:
Getting root tuber of aromatic turmeric raw material 10kg pulverizes, add 10L water and 30g amylase to mix thoroughly, be heated to 50 ℃, insulation enzymolysis 5 hours, then add 60L saturated limewater solution supersound extraction 30 minutes, extract 3 times, after extracting solution adjusting ph6, add in HZ816 macroporous resin and adsorb, with 5 times of amount 20% ethanolic soln wash-out impurity, again by 7 times of amount 60% ethanolic soln wash-out effective constituents, the concentrated placement of elutriant crystallization, after crystallisate dissolves with 0.2% aqueous sodium carbonate, add 3 times of amount 95% ethanolic solns, after filtration, regulate ph3 to place crystallization, crystallisate is used 75% ethanolic soln crystallization again, be dried to obtain Alpinetin 11g, content 97.5%.
Embodiment 3:
Getting root tuber of aromatic turmeric raw material 10kg pulverizes, add 10L water and 20g cellulase to mix thoroughly, be heated to 55 ℃, insulation enzymolysis 4 hours, then add 100L saturated limewater solution supersound extraction 30 minutes, extract 2 times, after extracting solution adjusting ph5, add in ADS-21 macroporous resin and adsorb, with 5 times of amount 30% ethanolic soln wash-out impurity, again by 7 times of amount 70% ethanolic soln wash-out effective constituents, the concentrated placement of elutriant crystallization, after crystallisate dissolves with 0.2% aqueous sodium hydroxide solution, add 1 times of amount 95% ethanolic soln, after filtration, regulate ph2 to place crystallization, crystallisate is used 70% ethanolic soln crystallization again, be dried to obtain Alpinetin 15g, content 95.4%.
Embodiment 4:
Getting root tuber of aromatic turmeric raw material 10kg pulverizes, add 10L water and 20g cellulase to mix thoroughly, be heated to 55 ℃, insulation enzymolysis 4 hours, then add 100L saturated limewater solution supersound extraction 30 minutes, extract 2 times, after extracting solution adjusting ph5, add in NK-9 macroporous resin and adsorb, with 5 times of amount 30% ethanolic soln wash-out impurity, again by 7 times of amount 70% ethanolic soln wash-out effective constituents, the concentrated placement of elutriant crystallization, after crystallisate dissolves with 0.2% aqueous sodium hydroxide solution, add 1 times of amount 95% ethanolic soln, after filtration, regulate ph2 to place crystallization, crystallisate is used 70% ethanolic soln crystallization again, be dried to obtain Alpinetin 12g, content 98.3%.
Claims (4)
1. from root tuber of aromatic turmeric, extract a method for Alpinetin, it is characterized in that: get root tuber of aromatic turmeric raw material pulverizing, add equal-volume water and biological enzyme, insulation enzymolysis 3-5 hour, then add saturated limewater solution supersound extraction 2-3 time, after extracting solution adjusting ph5-6, add in macroporous resin and adsorb, ethanolic soln gradient elution, the concentrated placement of elutriant crystallization, crystallisate dissolves and adds appropriate ethanol with buck, after filtration, regulates ph1-3 to place crystallization, crystallisate is used ethanolic soln crystallization again, is drying to obtain.
2. the method for extracting Alpinetin from root tuber of aromatic turmeric as claimed in claim 1, is characterized in that the described optional cellulase of enzyme, polygalacturonase and amylase, the 0.1-0.3% that consumption is amount of solid.
3. the method for extracting Alpinetin from root tuber of aromatic turmeric as claimed in claim 1, is characterized in that described macroporous resin is a kind of in AB-8, HZ816, ADS-21 and NK-9.
4. the method for extracting Alpinetin from root tuber of aromatic turmeric as claimed in claim 1, is characterized in that described ethanolic soln gradient elution is: 3-5 doubly measures 20-50% ethanolic soln wash-out impurity, more doubly measures 60-70% ethanolic soln wash-out effective constituent with 5-10.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410450660.XA CN104193715A (en) | 2014-09-05 | 2014-09-05 | Method for extracting alpinetin from curcuma aromatica |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410450660.XA CN104193715A (en) | 2014-09-05 | 2014-09-05 | Method for extracting alpinetin from curcuma aromatica |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104193715A true CN104193715A (en) | 2014-12-10 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410450660.XA Pending CN104193715A (en) | 2014-09-05 | 2014-09-05 | Method for extracting alpinetin from curcuma aromatica |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112772776A (en) * | 2021-01-08 | 2021-05-11 | 江西嘉博生物工程有限公司 | Preparation method and application of turmeric extract |
-
2014
- 2014-09-05 CN CN201410450660.XA patent/CN104193715A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112772776A (en) * | 2021-01-08 | 2021-05-11 | 江西嘉博生物工程有限公司 | Preparation method and application of turmeric extract |
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| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20141210 |
|
| WD01 | Invention patent application deemed withdrawn after publication |