CN104188861A - Complex enzyme mouthwash for eliminating dental plaque and preparation method thereof - Google Patents
Complex enzyme mouthwash for eliminating dental plaque and preparation method thereof Download PDFInfo
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- CN104188861A CN104188861A CN201410425069.9A CN201410425069A CN104188861A CN 104188861 A CN104188861 A CN 104188861A CN 201410425069 A CN201410425069 A CN 201410425069A CN 104188861 A CN104188861 A CN 104188861A
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Abstract
The invention belongs to the technical field of oral care, and discloses complex enzyme mouthwash for eliminating dental plaque and a preparation method thereof. Per 1000mL of complex enzyme mouthwash is prepared from the following components: 0.25-10mg of beta-glucanase (greater than or equal to 33U/mg), 0.25-10mg of alpha-amylase (greater than or equal to 50U/mg), 2.5-10mL of peppermint extract, 10g of edible salt and the balance of water. The mouthwash also comprises edible preservative. The complex enzyme mouthwash prepared by the method has a remarkable effect for removing dental plaque, is capable of effectively preventing occurrence of decayed tooth and gingivitis, is safe and non-toxic, and is mild and non-irritative on an oral cavity; and the preparation process is simple and easy to operate.
Description
Technical field
The invention belongs to oral care techniques field, relate to a kind of collutory, particularly a kind of compound enzyme collutory of subduing dental plaque and preparation method thereof.
Background technology
Dental plaque is oral cavity flora, particularly the biomembrane group that forms on tooth with the closely-related Streptococcus mutans of dental caries; be rich in the polysaccharide component such as glucosan and levan; can improve wherein antibacterial tolerance and the extraneous ability of coercing of antagonism, be equivalent to " hotbed " and " umbrella " of oral cavity bacterium growth.Once dental plaque forms in a large number, will contribute to growing fast of the cariogenic antibacterial in oral cavity, cause dental caries, gingivitis and other oral diseases, can increase what is more the risk of suffering from other general diseases.Current oral rinse oral fluid is paid close attention to the elimination effect to oral cavity bacterium individuality mostly, carrys out bactericidal as added lysozyme, and less concern is to forming the removing effect of biomembrane group-dental plaque.
Summary of the invention
In order to overcome the shortcoming and defect of prior art, the object of the present invention is to provide a kind of compound enzyme collutory of subduing dental plaque.The present invention selects the proportioning of suitable enzyme and various enzymes, and the collutory of preparation can effectively be removed dental plaque, nurses for mouth cavity daily.
Another object of the present invention is to provide the preparation method of the above-mentioned compound enzyme collutory of subduing dental plaque.
Object of the present invention is achieved through the following technical solutions:
Subdue a compound enzyme collutory for dental plaque, comprise 1,4 beta-glucanase (>=33U/mg), α-amylase (>=50U/mg), Folium Menthae extract, Sal and water.
In described compound enzyme collutory, the compound enzyme collutory that is every 1000ml with magnitude relation of each material comprises the 1,4 beta-glucanase of 0.25～10mg, the α-amylase of 0.25～10mg, the Folium Menthae extract of 2.5～10mL, Sal and the excess water of 10g.
The described compound enzyme collutory of subduing dental plaque, also comprises additive; Described additive is food antiseptics.
Described food antiseptics is potassium sorbate, and the consumption of described potassium sorbate is 0.01～0.02% of enzyme, extract, Sal and water gross mass.
The preparation method of described Folium Menthae extract is for to add Herba Menthae in alcoholic solution, and homogenizing is centrifugal, gets supernatant, and rotary evaporation is concentrated, obtains Herba Menthae concentrate; In Herba Menthae concentrate, add water dissolution, store, obtain Folium Menthae extract.
The mass volume ratio of described Herba Menthae and alcoholic solution is 2.0g:30mL; Volumes of aqueous ethanol concentration is 100%.
The mass volume ratio of described Herba Menthae and water is 2.0g:10mL.
Described water is reverse-osmosis pure water.
Described homogenizing adopts high-shear homogenizer to carry out homogenizing, and the rotating speed of homogenizing is 10000rpm, and homogenizing time is 10min; Described centrifugal rotational speed is 5000rpm, and centrifugation time is 10min;
The instrument that described evaporation and concentration adopts is Rotary Evaporators, and at 40 DEG C, rotary evaporation in vacuo is concentrated in Herba Menthae concentrate does not have flowing liquid; The temperature of described storage is-40 DEG C.
The preparation method of the described compound enzyme collutory of subduing dental plaque, comprises the following steps:
(1) by soluble in water to soluble in water to 1,4 beta-glucanase, α-amylase, Folium Menthae extract and Sal or described four kinds of materials and food antiseptics, obtain mixed liquor;
(2) by centrifugal mixed liquor in step (1), filter, preserve, obtain subduing the compound enzyme collutory of dental plaque.
Described in step (1), water is reverse-osmosis pure water;
Centrifugal condition described in step (2) is that centrifugal rotational speed is 5000～10000rpm, and centrifugation time is 5～10min, and centrifuging temperature is 4～25 DEG C;
Described in step (2), filter the membrane filtration that adopts sterilizing, the aperture of filter membrane is 0.22 μ m; Filtrate after filtration is placed in 4 DEG C of Refrigerator stores.
Single-minded 1,3 and the Isosorbide-5-Nitrae glycosidic bond that acts on beta glucan of 1,4 beta-glucanase, oligosaccharide and the glucose of 3 to 5 glucose units of generation.α-amylase (α-Isosorbide-5-Nitrae-D-glucan hydrolase) acts on α-Isosorbide-5-Nitrae-glucosan, indistinguishably cuts off at random the α-Isosorbide-5-Nitrae-glycosidic bond of sugar chain inside, removes the starchy material in food debris, suppresses the formation of dental plaque; On the other hand, there are some researches show that α-amylase can specificly be combined with streptococcus, therefore α-amylase may affect the streptococcic field planting in dental plaque.
Compared with prior art, tool of the present invention has the following advantages and effect:
(1) to remove dental plaque effect remarkable for the prepared compound enzyme collutory of the present invention, again simultaneously can fresh breath;
(2) described compound enzyme collutory safety non-toxic, non-stimulated to oral cavity gentleness; And the acid-base value of compound enzyme collutory is that pH value is 7.2-7.6, noresidue, pollution-free;
(3) production technology of the present invention is simple, easily realizes.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is done to further detailed description, but embodiments of the present invention are not limited to this.
Embodiment 1
(1) preparation method of Folium Menthae extract: in 30mL alcoholic solution (mass percent concentration is 100%, 4 DEG C of pre-cooling), add the Herba Menthae blade that 2.0g is dry, in high-shear homogenizer with the rotating speed homogenizing 10min of 10000rpm; Adopt the rotating speed centrifugal 10min of centrifuge with 5000rpm, collect supernatant.Supernatant is concentrated in concentrate and there is no flowing liquid in Rotary Evaporators, obtain Herba Menthae concentrate, with 10mL reverse osmosis pure water dissolution Herba Menthae concentrate, after subpackage, store for future use in-40 DEG C;
(2) by the 1,4 beta-glucanase of 0.25mg (>=33U/mg), the α-amylase (>=50U/mg) of 0.25mg, 2.5mL Folium Menthae extract, 0.1g potassium sorbate and 10g Sal join in reverse-osmosis pure water, mix, continue subsequently to add reverse-osmosis pure water until mixed liquor cumulative volume is 1000mL, subsequently mixed liquor is carried out to centrifugal (centrifugal rotational speed is 5000rpm, centrifugation time is 10min, centrifuging temperature is 25 DEG C), 0.22 μ m membrane filtration, puts into filtrate the refrigerator storage of 4 DEG C, obtains subduing the compound enzyme collutory of dental plaque.The pH value of prepared collutory is 7.2～7.6.
Embodiment 2
(1) preparation method of Folium Menthae extract is with embodiment 1;
(2) by 0.5mg 1,4 beta-glucanase (>=33U/mg), 0.5mg α-amylase (>=50U/mg), 2.5mL Folium Menthae extract, 0.1g potassium sorbate and 10g Sal join in reverse-osmosis pure water, mix, continue to add reverse-osmosis pure water until mixed liquor cumulative volume is 1000mL, subsequently mixed liquor is carried out to centrifugal (centrifugal rotational speed is 5000rpm, centrifugation time is 10min, centrifuging temperature is 25 DEG C), 0.22 μ m membrane filtration, puts into filtrate the refrigerator storage of 4 DEG C, obtains subduing the compound enzyme collutory of dental plaque.The pH value of prepared collutory is 7.2～7.6.
Embodiment 3
(1) preparation method of Folium Menthae extract is with embodiment 1;
(2) by 1mg 1,4 beta-glucanase (>=33U/mg), 1mg α-amylase (>=50U/mg), 5mL Folium Menthae extract and 10g Sal join in reverse-osmosis pure water, mix, add 0.2g potassium sorbate, continue subsequently to add reverse-osmosis pure water until mixed liquor cumulative volume is 1000mL, subsequently mixed liquor is carried out to centrifugal (centrifugal rotational speed is 8000rpm, centrifugation time is 8min, centrifuging temperature is 20 DEG C), 0.22 μ m membrane filtration, puts into filtrate the refrigerator storage of 4 DEG C, obtains subduing the compound enzyme collutory of dental plaque.The pH value of prepared collutory is 7.2～7.6.
Embodiment 4
(1) preparation method of Folium Menthae extract is with embodiment 1;
(2) by 2.5mg 1,4 beta-glucanase (>=33U/mg), 2.5mg α-amylase (>=50U/mg), 5mL Folium Menthae extract and 10g Sal join in reverse-osmosis pure water, mix, add 0.2g potassium sorbate, continue to add reverse-osmosis pure water until mixed liquor cumulative volume is 1000mL, subsequently mixed liquor is carried out to centrifugal (centrifugal rotational speed is 8000rpm, centrifugation time is 8min, centrifuging temperature is 15 DEG C), 0.22 μ m membrane filtration, puts into filtrate the refrigerator storage of 4 DEG C, obtains subduing the compound enzyme collutory of dental plaque.The pH value of prepared collutory is 7.2～7.6.
Embodiment 5
(1) preparation method of Folium Menthae extract is with embodiment 1;
(2) by 5mg 1,4 beta-glucanase (>=33U/mg), 5mg α-amylase (>=50U/mg), 10mL Folium Menthae extract, 10g Sal and 0.1g potassium sorbate join in reverse-osmosis pure water, mix, continue to add reverse-osmosis pure water until mixed liquor cumulative volume is 1000mL, subsequently mixed liquor is carried out to centrifugal (centrifugal rotational speed is 10000rpm, centrifugation time is 5min, centrifuging temperature is 10 DEG C), 0.22 μ m membrane filtration, puts into filtrate the refrigerator storage of 4 DEG C, obtains subduing the compound enzyme collutory of dental plaque.The pH value of prepared collutory is 7.2～7.6.
Embodiment 6
(1) preparation method of Folium Menthae extract is with embodiment 1;
(2) by 10mg 1,4 beta-glucanase (>=33U/mg), 10mg α-amylase (>=50U/mg), 10mL Folium Menthae extract, 0.1g potassium sorbate and 10g Sal join in reverse-osmosis pure water, mix, continue to add reverse-osmosis pure water until mixed liquor cumulative volume is 1000mL, subsequently mixed liquor is carried out to centrifugal (centrifugal rotational speed is 10000rpm, centrifugation time is 5min, centrifuging temperature is 4 DEG C), 0.22 μ m membrane filtration, puts into filtrate the refrigerator storage of 4 DEG C, obtains subduing the compound enzyme collutory of dental plaque.The pH value of prepared collutory is 7.2～7.6.
Measure of merit:
Choose at random 20 student enrollment volunteers by inclusive criteria.Inclusive criteria: whole body health in order; Complete mouthful must not be less than 20 function teeth; Test in first 3 months and do not take antibiotic; Tested tooth and adjacent teeth are without inclination, abnormal development, periodontal pocket and gingival recession, and without hat and fixed bridge reparation and wearing orthodontic appliance, facing and root face damage without charges and dental caries, are substituted with offside tooth of the same name or homonymy adjacent teeth if examine anodontia; Oral mucosa, gingiva change without pathologic; Without smoking and excessive drinking custom; Do not participate in other similar experimental studies simultaneously.
Experiment experimenter on the same day carries out normal diet after oral cavity cleaning as requested in the morning, until no longer carry out any oral cavity cleaning measure before experiment, at 10 in evening formally starts experiment.Experimenter first dips appropriate dental plaque with sterilized cotton swabs and shows that liquid (main component is erythrosine) is coated in to be examined gently and on facing, carry out dental plaque dyeing, stop 30 seconds, then gargle three times with clear water, according to the Q-H plaque index score standard of Tureskey improvement, Ramfjord index tooth (l6,21,24,36,41,44) bacterial plaque of tooth surface area is carried out to the score of plaque index value.Score standard is as follows: 0-facing is without bacterial plaque, there is the point-like bacterial plaque being dispersed at gum edge place of 1-neck portion, 2-neck portion bacterial plaque width is no more than 1mm, 3-neck portion bacterial plaque cover width exceedes 1mm, but at facing below 1/3,4-bacterial plaque area coverage accounts between facing 1/3 and 2/3, and 5-bacterial plaque area coverage accounts for facing more than 2/3.Every experimenter's plaque index value score is that the score sum of 6 teeth is divided by 6.The first plaque index of the buccal labial surface of 6 Ramfjord index teeth of each experimenter is I
1, use given collutory to carry out rinsing the mouth 2min, each 20mL.After rinsing the mouth finishes, gargle three times with clear water again, adopt the rear plaque index I of same procedure inspection record
2.The reduction of dental plaque is I
1-I
2.Every single experiment finishes the measure of rear recovery daily cleaning, residual action and impact that before eliminating, a kind of collutory exists.After three days, use another kind of collutory to test, method is the same.
Adopt the compound enzyme collutory of embodiment 1～6 preparation, taking only containing Sal and water without enzyme group as matched group, follow single-blind randomized own control principle, add up 20 university student volunteers and uses plaque index change situation before and after collutory, test result is as shown in table 1.
The impact of table 1 compound enzyme collutory on dental plaque
As can be seen from the table, use embodiment 1～6 prepared compound enzyme collutory, dental plaque reduction is all higher than without enzyme group, and along with the increasing of enzyme content, dental plaque reduction increases, and presents dose-dependence.With compared with enzyme group, use the reduction of compound enzyme collutory Dental Plaque all to have significant increase, p-value value is all less than 0.001, and the prepared compound enzyme collutory of the present invention has the effect of significant removing dental plaque.
Above-described embodiment is preferably embodiment of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under spirit of the present invention and principle, substitutes, combination, simplify; all should be equivalent substitute mode, within being included in protection scope of the present invention.
Claims (10)
1. a compound enzyme collutory of subduing dental plaque, is characterized in that: comprise 1,4 beta-glucanase, α-amylase, Folium Menthae extract, Sal and water.
2. the compound enzyme collutory of subduing according to claim 1 dental plaque, is characterized in that: in described compound enzyme collutory, the compound enzyme collutory that is every 1000mL with magnitude relation of each material comprises the 1,4 beta-glucanase of 0.25～10mg, the α-amylase of 0.25～10mg, the Folium Menthae extract of 2.5～10mL, Sal and the excess water of 10g.
3. the compound enzyme collutory of subduing according to claim 1 dental plaque, is characterized in that: described in subdue the compound enzyme collutory of dental plaque, also comprise additive; Described additive is food antiseptics.
4. the compound enzyme collutory of subduing according to claim 3 dental plaque, is characterized in that: described food antiseptics is potassium sorbate, and the consumption of described potassium sorbate is 0.01～0.02% of enzyme, extract, Sal and water gross mass.
5. the preparation method of subduing according to claim 1 the compound enzyme collutory of dental plaque, is characterized in that: comprise the following steps:
(1) by soluble in water to 1,4 beta-glucanase, α-amylase, Folium Menthae extract and Sal or by soluble in water to described four kinds of materials and food antiseptics, obtain mixed liquor;
(2) by centrifugal mixed liquor in step (1), filter, preserve, obtain eliminating the compound enzyme collutory of dental plaque.
6. the preparation method of subduing according to claim 5 the compound enzyme collutory of dental plaque, is characterized in that: the preparation method of Folium Menthae extract described in step (1) is for to add Herba Menthae in alcoholic solution, homogenizing, centrifugal, get supernatant, rotary evaporation is concentrated, obtains Herba Menthae concentrate; In Herba Menthae concentrate, add water dissolution, store, obtain Folium Menthae extract.
7. the preparation method of subduing according to claim 6 the compound enzyme collutory of dental plaque, is characterized in that: the mass volume ratio of described Herba Menthae and alcoholic solution is 2.0g:30mL; Volumes of aqueous ethanol concentration is 100%;
The mass volume ratio of described Herba Menthae and water is 2.0g:10mL; Described water is reverse-osmosis pure water.
8. the preparation method of subduing according to claim 6 the compound enzyme collutory of dental plaque, is characterized in that: the instrument that described evaporation and concentration adopts is Rotary Evaporators, and rotary evaporation in vacuo is concentrated in Herba Menthae concentrate does not have flowing liquid.
9. subdue according to claim 5 the preparation method of the compound enzyme collutory of dental plaque, it is characterized in that: centrifugal condition described in step (2) is that centrifugal rotational speed is 5000～10000rpm, centrifugation time is 5～10min, and centrifuging temperature is 4～25 DEG C;
Described in step (1), water is reverse-osmosis pure water.
10. the preparation method of subduing according to claim 5 the compound enzyme collutory of dental plaque, is characterized in that: described in step (2), filter the membrane filtration that adopts sterilizing, the aperture of filter membrane is 0.22 μ m.
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| CN201410425069.9A CN104188861A (en) | 2014-08-26 | 2014-08-26 | Complex enzyme mouthwash for eliminating dental plaque and preparation method thereof |
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| CN201410425069.9A CN104188861A (en) | 2014-08-26 | 2014-08-26 | Complex enzyme mouthwash for eliminating dental plaque and preparation method thereof |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5871966A (en) * | 1994-05-11 | 1999-02-16 | Novo Nordisk A/S | Enzyme with endo-1,3(4)-β- Glucanase activity |
| CN1264305A (en) * | 1997-06-17 | 2000-08-23 | 诺沃挪第克公司 | Oral care composition comprising bacillus pullulanase and dextranase |
| CN1275906A (en) * | 1997-10-17 | 2000-12-06 | 诺沃挪第克公司 | Plaque-inhibiting oral compositions |
| CN1688282A (en) * | 2002-08-28 | 2005-10-26 | 高露洁-棕榄公司 | Dual component dental composition containing enzyme |
| CN102871897A (en) * | 2012-08-31 | 2013-01-16 | 青岛康泰鑫环保科技有限公司 | Peppermint tooth care mouth wash |
-
2014
- 2014-08-26 CN CN201410425069.9A patent/CN104188861A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5871966A (en) * | 1994-05-11 | 1999-02-16 | Novo Nordisk A/S | Enzyme with endo-1,3(4)-β- Glucanase activity |
| CN1264305A (en) * | 1997-06-17 | 2000-08-23 | 诺沃挪第克公司 | Oral care composition comprising bacillus pullulanase and dextranase |
| CN1275906A (en) * | 1997-10-17 | 2000-12-06 | 诺沃挪第克公司 | Plaque-inhibiting oral compositions |
| CN1688282A (en) * | 2002-08-28 | 2005-10-26 | 高露洁-棕榄公司 | Dual component dental composition containing enzyme |
| CN102871897A (en) * | 2012-08-31 | 2013-01-16 | 青岛康泰鑫环保科技有限公司 | Peppermint tooth care mouth wash |
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