CN104186328A - Rapid propagation method for tissue culture of grewia biloba - Google Patents
Rapid propagation method for tissue culture of grewia biloba Download PDFInfo
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- CN104186328A CN104186328A CN201410462964.8A CN201410462964A CN104186328A CN 104186328 A CN104186328 A CN 104186328A CN 201410462964 A CN201410462964 A CN 201410462964A CN 104186328 A CN104186328 A CN 104186328A
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- bilobed grewia
- grewia
- bilobed
- aseptic
- group training
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Abstract
The invention discloses a rapid propagation method for grewia biloba tissue culture. The rapid propagation method comprises the steps of acquisition of aseptic seedlings, propagation of multiple shoots, rooting culture, acclimatization and transplant and the like. The grewia biloba prepared by the method is good in multiplication coefficient, high in survival rate, short in growth cycle and simple in operation, and a large amount of grewia biloba seedlings can be obtained in a short period of time.
Description
Technical field
The present invention relates to the quick-breeding method that bilobed grewia tissue is cultivated, belong to plant technology field.
Background technology
Bilobed grewia, Tiliaceae,
grewia biloba G.Don, have another name called: bilobed grewia root stem and leaf, sesame candy fruit, month pearlite layer, Grewia biloba G.Don, grow in hills, meadow, limit, low hill path, shrubbery or sparse woods; Machaka or dungarunga, up to 3 meters of left and right; Sprig has stellate hair.Avette or the narrow rhombus of the narrow water chestnut shape of leaf, long 3-9 centimetre, wide l-4 centimetre, the close raw denticle in edge, surface is several without hair, and raw stellate hair or several without hair is dredged at the back side, and base goes out 3, arteries and veins, the long 2-6 millimeter of petiole.Cyme and leaf be to life, flower pistac, and diameter is less than 1 centimetre, sepal 5, narrow lanceolar, is about 5 millimeters, the close raw grey undercoat in outside, inner face is without hair, and petal 5, is about 1.2 millimeters; Stamen majority, flower pesticide white, style is long, ovary hairiness.Drupe is orange red, diameter 7-12 millimeter, and without hair, 2 split, and often split and have 2 cores, inside have seed 2-4 grain.The florescence 6-7 month, the fruit phase 8-9 month.Property strong, cold-resistant, drought-resistant, barren-resistant.Happiness light, also slightly resistance to the moon.Not tight to soil requirement, in the soil that is rich in humus, grow vigorous.There is distribution the provinces and regions such as Guangdong, Guangxi, Hunan, Jiangxi, Fujian, Taiwan, Zhejiang, Anhui.Be used as medicine with root or complete stool.Xia Qiu excavates, and clean section is dried.For infantile malnutrition, insufficiency of the spleenly rush down for a long time seminal emission, red collapsing, leukorrhea, the prolapse of uterus, prolapse of the anus, rheumatic arthritis.With sowing or division propagation.The main routine that adopts is planted at present, and tissue culture method can obtain a large amount of bilobed grewia seedling fast, keeps the stability of genetic character, overcomes the limitation of region.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method for quickly breeding of bilobed grewia, and good by the preparation-obtained bilobed grewia growth coefficient of the method, survival rate is high, and growth cycle is short, simple to operate, can obtain in a short time a large amount of bilobed grewia seedling.
Technical problem to be solved by this invention realizes by following scheme:
Get bilobed grewia non-hibernating eggs then, remove exosper, in bleaching powder, soak 3min, flowing water rinses 2-3h, on superclean bench, 75% alcohol is processed 30s, mercury chloride is processed 17min, aseptic water washing 5-7 time, blotting paper sucks surface moisture, the seed of disinfecting is inoculated in MS medium and is grown, the bilobed grewia aseptic seedling growing out cuts radicle position, access culture medium prescription is to carry out adventitious buds proliferation cultivation in White+IBA0.1mg/L+6-BA1mg/L+ active carbon 1.5g/L inducing culture, additional saccharose 30g/L, agar 6.5g/L, PH5.8, illumination 3000lx, 26 DEG C of temperature, the Multiple Buds that propagation cultivates is out put into root media 1/3White+NAA0.2mg/L+ cerous nitrate 1mg/L, after rooting of vitro seedling, grow to 5cm, bilobed grewia test-tube plantlet is taken out, wash away root medium, soak 3s with thiophanate methyl, clear water cleans up, plant in the humus soil of crossing in sterilization treatment, be positioned in greenhouse, regularly water, after two weeks, be transplanted into land for growing field crops, after one month, add up survival rate.
The bilobed grewia survival rate that adopts the present invention to prepare is 92%, and the cycle is short, and output is large, and less energy consumption is beneficial to implant mass.
Below in conjunction with embodiment, the present invention is further elaborated, but the scope of protection of present invention is not limited to following embodiment.
Embodiment
Embodiment 1
Get bilobed grewia non-hibernating eggs then, remove exosper, in bleaching powder, soak 3min, flowing water rinses 2-3h, on superclean bench, 75% alcohol is processed 30s, mercury chloride is processed 17min, aseptic water washing 5-7 time, blotting paper sucks surface moisture, the seed of disinfecting is inoculated in MS medium and is grown, the bilobed grewia aseptic seedling growing out cuts radicle position, access culture medium prescription is to carry out adventitious buds proliferation cultivation in White+IBA0.1mg/L+6-BA1mg/L+ active carbon 1.5g/L inducing culture, additional saccharose 30g/L, agar 6.5g/L, PH5.8, illumination 3000lx, 26 DEG C of temperature, the Multiple Buds that propagation cultivates is out put into root media 1/3White+NAA0.05mg/L+ cerous nitrate 0.5mg/L, after rooting of vitro seedling, grow to 5cm, bilobed grewia test-tube plantlet is taken out, wash away root medium, soak 3s with thiophanate methyl, clear water cleans up, plant in the humus soil of crossing in sterilization treatment, be positioned in greenhouse, regularly water, after two weeks, be transplanted into land for growing field crops, after one month, add up survival rate, reach 89%.
Embodiment 2
Get bilobed grewia non-hibernating eggs then, remove exosper, in bleaching powder, soak 3min, flowing water rinses 2-3h, on superclean bench, 75% alcohol is processed 30s, mercury chloride is processed 17min, aseptic water washing 5-7 time, blotting paper sucks surface moisture, the seed of disinfecting is inoculated in MS medium and is grown, the bilobed grewia aseptic seedling growing out cuts radicle position, access culture medium prescription is to carry out adventitious buds proliferation cultivation in White+IBA0.1mg/L+6-BA1mg/L+ active carbon 1.5g/L inducing culture, additional saccharose 30g/L, agar 6.5g/L, PH5.8, illumination 3000lx, 26 DEG C of temperature, the Multiple Buds that propagation cultivates is out put into root media 1/3White+NAA0.2mg/L+ cerous nitrate 1.5mg/L, after rooting of vitro seedling, grow to 5cm, bilobed grewia test-tube plantlet is taken out, wash away root medium, soak 3s with thiophanate methyl, clear water cleans up, plant in the humus soil of crossing in sterilization treatment, be positioned in greenhouse, regularly water, after two weeks, be transplanted into land for growing field crops, after one month, add up survival rate, reach 88%.
Embodiment 3
Get bilobed grewia non-hibernating eggs then, remove exosper, in bleaching powder, soak 3min, flowing water rinses 2-3h, on superclean bench, 75% alcohol is processed 30s, mercury chloride is processed 17min, aseptic water washing 5-7 time, blotting paper sucks surface moisture, the seed of disinfecting is inoculated in MS medium and is grown, the bilobed grewia aseptic seedling growing out cuts radicle position, access culture medium prescription is to carry out adventitious buds proliferation cultivation in White+IBA0.1mg/L+6-BA1mg/L+ active carbon 1.5g/L inducing culture, additional saccharose 30g/L, agar 6.5g/L, PH5.8, illumination 3000lx, 26 DEG C of temperature, the Multiple Buds that propagation cultivates is out put into root media 1/3White+NAA0.1mg/L+ cerous nitrate 1mg/L, after rooting of vitro seedling, grow to 5cm, bilobed grewia test-tube plantlet is taken out, wash away root medium, soak 3s with thiophanate methyl, clear water cleans up, plant in the humus soil of crossing in sterilization treatment, be positioned in greenhouse, regularly water, after two weeks, be transplanted into land for growing field crops, after one month, add up survival rate, reach 90%.
Claims (5)
1. a method for quickly breeding for bilobed grewia group training, comprises propagation, the culture of rootage of induction, the Multiple Buds of acquisition, the axillalry bud of aseptic explant, the regulation and control of test-tube plantlet, and its key step is as follows:
(1) get the seed that bilobed grewia is given birth to then, on superclean bench to its disinfection;
(2) getting seed that step (1) disinfects inoculates in MS medium and grows;
(3) get the bilobed grewia aseptic seedling growing out in step (2) and cut radicle position, access culture medium prescription is to carry out adventitious buds proliferation cultivation in White+IBA0.1mg/L+6-BA1mg/L+ active carbon 1.5g/L inducing culture, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 3000lx, 26 DEG C of temperature;
(4) getting step (3) breeds the Multiple Buds of cultivating out and puts into root media 1/3White+NAA0.05-0.2mg/L+ cerous nitrate 0.5-1.5mg/L;
(5) the bilobed grewia test-tube plantlet after taking root carries out hardening cultivation.
2. according to the method for quickly breeding of a kind of bilobed grewia group training claimed in claim 1, it is characterized in that: the acquisition of bilobed grewia aseptic seed described in step (1) is; get bilobed grewia non-hibernating eggs then; remove exosper, soak 3min in bleaching powder, flowing water rinses 2-3h; on superclean bench, 75% alcohol is processed 30s; mercury chloride is processed 17min, aseptic water washing 5-7 time, and blotting paper sucks surface moisture.
3. according to the method for quickly breeding of a kind of bilobed grewia group training claimed in claim 1, it is characterized in that: in step (3) proliferated culture medium, added active carbon 1.5g/L, can prevent the browning in breeding.
4. according to the method for quickly breeding of a kind of bilobed grewia group training claimed in claim 1, it is characterized in that: in the root media in step (4), add cerous nitrate, rare earth can hestening rooting and elongation growth.
5. according to the method for quickly breeding of a kind of bilobed grewia group training claimed in claim 1, it is characterized in that: in step (5), the acclimatization and transplants method of bilobed grewia is, bilobed grewia test-tube plantlet is taken out, wash away root medium, soak 3s with thiophanate methyl, clear water cleans up, plant in the humus soil of crossing in sterilization treatment, be positioned in greenhouse, regularly water, after two weeks, be transplanted into land for growing field crops, after one month, add up survival rate.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410462964.8A CN104186328A (en) | 2014-09-12 | 2014-09-12 | Rapid propagation method for tissue culture of grewia biloba |
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| CN201410462964.8A CN104186328A (en) | 2014-09-12 | 2014-09-12 | Rapid propagation method for tissue culture of grewia biloba |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104737905A (en) * | 2015-02-14 | 2015-07-01 | 涂湘炎 | Millettia specisoa tissue culture rooting method |
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- 2014-09-12 CN CN201410462964.8A patent/CN104186328A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104737905A (en) * | 2015-02-14 | 2015-07-01 | 涂湘炎 | Millettia specisoa tissue culture rooting method |
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Application publication date: 20141210 |