CN104181139A - Phytoplankton fluorescence signal detection method - Google Patents

Abstract

The invention relates to the field of fluorescence signal detection, in particular to a phytoplankton fluorescence signal detection method. The detection method comprises the following steps: A, acquiring a phytoplankton fluorescence signal; B, carrying out amplification treatment on the acquired simulated fluorescence signal; C, performing conversion treatment of analog and digital signals on n parallel paths of analog signals after gain amplification to convert the analog signals into digital signals; and D, performing data processing on the n converted digital signals. The phytoplankton fluorescence signal detection method can detect the phytoplankton fluorescence signal with high precision and wide range.

Description

A kind of phytoplankton fluorescence signal detection method

Technical field

The present invention relates to a kind of fluorescence signal detection field, the fluorescence signal detection method of especially a kind of phytoplankton.

Background technology

During traditional fluorescence signal detects, the testing circuit that adopts is to utilize photomultiplier Acquisition Circuit that fluorescence signal is amplified, then after processing by mimic channel, sends into high-precision analog and digital signal converter (below all referred to as AD converter) and realized the high-precision digital that gathers fluorescence signal.The precise digitization of sort circuit depends on high-precision AD converter, due to single AD converter, effectively to input analog signal range limited, therefore limit the output signal of analog signal processing circuit, and then had influence on the accuracy of detection of the fluorescence signal of this circuit input.A kind of signal processing circuit for vitamin fluoroscopic examination for example proposing in patent CN 201569629 U is exactly to use single AD converter.Simultaneously because system is to high-precision requirement, the conversion figure place of the high-precision AD converter part of selecting is conventionally more than 24bit, the AD converter precision of this class is high, and actual voltage or the current value that can differentiate of circuit is too low, designing requirement to simulating signal is higher, and some of mimic channel device itself are disturbed and easily introduced noise effect to actual acquisition precision.

The detection method of traditional phytoplankton fluoroscopic examination circuit is similar to the form of the signal processing circuit of said vitamin fluoroscopic examination, carrying out phytoplankton fluorescence signal with traditional fluorescence signal testing circuit detects and cannot meet that (phytoplankton type is various to phytoplankton, body size diversity factor is very large) the high requirement of fluorescence signal quality, it is very low that it reaches several magnitude fluorescence signal responsivenesses to variation, the signal that only falls into valid analysing range could be realized high-precision signal and detect, and detected value often occurs too low or exceed the phenomenon of detected value.And because the non-repeated of on-the-spot phytoplankton fluorescence (is different from other and adopts container Static Detection mode, on-the-spot phytoplankton fluoroscopic examination can only adopt flowing seawater to continue the mode that flows into and detect in real time), make to adopt traditional fluorescence signal testing circuit to carry out phytoplankton fluoroscopic examination and more easily produced undetected situation.

Summary of the invention

For above-mentioned prior art, the technical matters that the present invention solves is to provide a kind of phytoplankton fluorescence signal detection method, and it is wider that this detection method has improved range higher to the accuracy of detection of phytoplankton fluorescence signal and that measure.

For addressing the above problem, phytoplankton fluorescence signal detection method of the present invention, comprises the steps:

A, the step of collection phytoplankton fluorescence signal;

B, the fluorescence signal of the simulation of collection is amplified to the step of processing, specifically: first the fluorescence signal of this simulation is carried out to first order gain and amplify, signal after again this first order being amplified divides that n road is parallel carries out respectively second level gain and amplify, and this n road is parallel carries out the gain that second level gain amplifies and be respectively different f1～fn;

C, the simulating signal after the gain of parallel n road is amplified is carried out analog and digital signal conversion process respectively and converts to the step of digital signal, and the conversion value of the n a changing digital signal is AD1～ADn;

D, the digital signal after n is changed is carried out the step of data processing, specifically: determine different weight size k1～kn according to the numerical values recited of n conversion value AD1～And, thereby testing result is calculated as:

$\mathrm{AD}=\frac{k_1\×\frac{{\mathrm{AD}}_1}{f_1}+k_2\×\frac{{\mathrm{AD}}_2}{f_2}+k_3\×\frac{{\mathrm{AD}}_3}{f_3}...+k_{n-1}\×\frac{{\mathrm{AD}}_{n-1}}{f_{n-1}}+k_n\×\frac{{\mathrm{AD}}_n}{f_n}}{k_1+k_2+k_3+...+k_{n-1}+k_n}.$

Wherein, after step C, enter and also increase a step C1 before step D: the step that n conversion value AD1～ADn carried out to data latch.

Wherein, this detection method can be used for detecting the phytoplankton fluorescence signal of m wave band.

Brief description of the drawings

Fig. 1 is circuit structure diagram of the present invention.

Fig. 2 is the structural representation of the first embodiment of the present invention.

Fig. 3 A is the Part I electrical block diagram of the second embodiment of the present invention.

Fig. 3 B is the Part II electrical block diagram of the second embodiment of the present invention.

Embodiment

Now the present invention is further described with embodiment by reference to the accompanying drawings.

Phytoplankton fluorescence signal detection method of the present invention, comprises the steps:

A, the step of collection phytoplankton fluorescence signal;

B, the fluorescence signal of the simulation of collection is amplified to the step of processing, specifically: first the fluorescence signal of this simulation is carried out to first order gain and amplify, signal after again this first order being amplified divides that n road is parallel carries out respectively second level gain and amplify, and this n road is parallel carries out the gain that second level gain amplifies and be respectively different f1～fn;

C, the simulating signal after the gain of parallel n road is amplified is carried out analog and digital signal conversion process respectively and converts to the step of digital signal, and the conversion value of the n a changing digital signal is AD1～ADn;

D, the digital signal after n is changed is carried out the step of data processing, specifically: determine different weight size k1～kn according to the numerical values recited of n conversion value AD1～And, thereby testing result is calculated as:

$\mathrm{AD}=\frac{k_1\×\frac{{\mathrm{AD}}_1}{f_1}+k_2\×\frac{{\mathrm{AD}}_2}{f_2}+k_3\×\frac{{\mathrm{AD}}_3}{f_3}...+k_{n-1}\×\frac{{\mathrm{AD}}_{n-1}}{f_{n-1}}+k_n\×\frac{{\mathrm{AD}}_n}{f_n}}{k_1+k_2+k_3+...+k_{n-1}+k_n}.$

Wherein, after step C, enter and also increase a step C1 before step D: the step that n conversion value AD1～And carried out to data latch.

Wherein, this detection method can be used for detecting the phytoplankton fluorescence signal of m wave band.

According to above-mentioned phytoplankton fluorescence signal detection method, propose a kind of for realizing the testing circuit of above-mentioned detection method below.

The present invention is based on above-mentioned detection method phytoplankton fluorescence signal testing circuit electrical block diagram as shown in Figure 1, comprising: photomultiplier Acquisition Circuit 20, analog signal processing circuit and digital processing circuit 30.

After gathering phytoplankton fluorescence signal, described photomultiplier Acquisition Circuit 20 passes to analog signal processing circuit;

Described analog signal processing circuit comprises: operational amplification circuit and analog and digital signal change-over circuit; Described operational amplification circuit is made up of two-stage calculation amplifying circuit, first order operational amplification circuit is converted to the current signal of photomultiplier Acquisition Circuit input voltage signal and amplifies, second level operational amplification circuit is made up of n parallel operational amplification circuit, and the gain of n operational amplification circuit is respectively f1～fn; N concurrent operation amplifying circuit in described second level operational amplification circuit is connected with analog and digital signal change-over circuit; Described analog and digital signal change-over circuit is made up of n AD converter, be connected with n concurrent operation amplifying circuit in the operational amplification circuit of the second level respectively, the n of n AD converter AD conversion value is AD1～ADn, and analog and digital signal change-over circuit is connected with digital processing circuit 30; Described digital processing circuit 30 is converted to suitable transformation result by certain algorithm by n AD conversion value; Described digital processing circuit 30 uses Weighted Average Algorithm to process n AD conversion value, described digital processing circuit 30 is determined different weight size k1～kn according to the numerical values recited of n AD conversion value of n AD converter conversion, and the result that this phytoplankton fluorescence signal testing circuit finally obtains is:

$\mathrm{AD}=\frac{k_1\×\frac{{\mathrm{AD}}_1}{f_1}+k_2\×\frac{{\mathrm{AD}}_2}{f_2}+k_3\×\frac{{\mathrm{AD}}_3}{f_3}...+k_{n-1}\×\frac{{\mathrm{AD}}_{n-1}}{f_{n-1}}+k_n\×\frac{{\mathrm{AD}}_n}{f_n}}{k_1+k_2+k_3+...+k_{n-1}+k_n}.$

In described digital processing circuit 30, weight size determines, if the numerical value of certain AD conversion value in n AD conversion value is maximum and do not exceed the upper limit of maximum conversion value, the conversion of applicable AD converter after the processing of the operational amplification circuit gain on this road of current red tide fluorescence signal is described, the weight maximum of this road red tide fluorescence signal so; If the numerical value minimum of certain AD conversion value in n AD conversion value, illustrates that current red tide fluorescence signal is not suitable for the conversion of AD converter after the processing of the operational amplification circuit gain on this road; If AD conversion value exceeds the conversion upper limit of AD converter, illustrate that the operational amplification circuit gain on this road has exceeded the conversion range of AD converter, the weight of this road red tide fluorescence signal is made as 0 so.

Embodiment 1:

Based on above-mentioned amplifier circuit principle and working method, the present invention preferably more concrete preferred embodiment further illustrates and of the present inventionly realizes circuit mode as shown in Figure 2, as a more concrete preferred embodiment, comprise photomultiplier Acquisition Circuit 20, analog signal processing circuit, latch cicuit and digital processing circuit 30 (in Fig. 2, not indicating), reference source circuit 40, crystal oscillating circuit 41, addressing circuit 42.

Described photomultiplier Acquisition Circuit 20 is connected with analog signal processing circuit;

Described analog signal processing circuit is made up of two-stage calculation amplifying circuit and analog and digital signal change-over circuit 112, described first order operational amplification circuit is made up of operational amplification circuit 101, the current signal that first order operational amplification circuit 101 is inputted photomultiplier Acquisition Circuit 20 is converted to voltage signal, second level operational amplification circuit is by operational amplification circuit 1021, operational amplification circuit 1022, the parallel composition of operational amplification circuit 1023, if: the gain of operational amplification circuit 1021 is f1=1/20, the gain of operational amplification circuit 1022 is f2=1, the gain of operational amplification circuit 1023 is f3=20, second level operational amplification circuit has just respectively increased the sensing range of 20 times by the signal of script first order operational amplification circuit conversion respectively separately up and down like this, described operational amplification circuit is all selected high precision, the AD706 chip of Low Drift Temperature, described analog and digital signal change-over circuit 112 is made up of AD converter circuit 1121, AD converter circuit 1122, AD converter circuit 1123, the conversion value of AD converter circuit 1121 is that the conversion value of AD1, AD converter circuit 1122 is that the conversion value of AD2, AD converter circuit 1123 is AD3, operational amplification circuit 1021 is connected with AD converter circuit 1121, operational amplification circuit 1022 is connected with AD converter circuit 1122, and operational amplification circuit 1023 is connected with AD converter circuit 1123, described AD converter circuit all adopts ADS8323 chip, AD converter circuit 1121, AD converter circuit 1122 is all connected with reference source circuit 40 with AD converter circuit 1123, the reference voltage that ensures each AD converter is consistent, by the clock signal pin clock of AD converter circuit 1121, after linking together, the clock signal pin clock of the clock signal pin clock of AD converter circuit 1122 and AD converter circuit 1123 accesses crystal oscillating circuit 41, ensure that 3 A/D convertor circuits adopt stable and identical clock source, AD converter circuit 1121, AD converter circuit 1122 and AD converter circuit 1123 are controlled and are opened by digital processing circuit 30.Because operational amplification circuit in the analog signal processing circuit in this embodiment and analog and digital signal change-over circuit are to adopt identical circuit structure and component parameters, thereby can ensure the time delay consistance of analog signal transmission.

Described latch cicuit is made up of three groups of latch cicuits, is respectively latch cicuit 501, latch cicuit 502 and latch cicuit 503, and each group latch cicuit includes two latch chips; A/D convertor circuit 1121 is connected with latch cicuit 501, and A/D convertor circuit 1122 is connected with latch cicuit 502, and A/D convertor circuit 1123 is connected with latch cicuit 503; Described latch cicuit is by the AD conversion value latch in analog and digital signal change-over circuit; The Enable Pin of described latch cicuit 501, latch cicuit 502 and latch cicuit 503 is all connected with addressing circuit 42; And then by addressing circuit 42, the addressing control of three groups of latch cicuits is read successively the laggard row data processing of latched value of three groups of latch cicuits by digital processing circuit 30.

Port AD_CS in control output end mouth and Fig. 2 of described digital processing circuit 30 (not shown in FIG.), AD_RD, AD_CONVED, AD_SEL_0, AD_SEL_1, AD_SEL_2 connects, its signal input port with port AD_BUS in Fig. 2 and the parallel bus port AD_[15.0 of 16] be connected, digital processing circuit 30 is for to the above-mentioned n of reading the laggard row data processing of AD signal conversion value (being mainly calculation process) and the process of above-mentioned signals collecting is controlled, can adopt control treatment circuit and the device of prior art to realize, as microcomputer control circuit, personal computer (PC), panel computer (PAD) etc.Digital processing circuit 30 is to adopt Weighted Average Algorithm to carry out data processing to the AD conversion value reading.For example: the gain f1=510/10K=0.051 of circuit in first analog channel, in second analog channel, the gain of circuit is f2=1, in the 3rd analog channel, the gain of circuit is f3=10K/510=19.6, the weighted index adopting in digital processing circuit 30 is made as k1:k2:k3=9:0.8:0.2, the definition of weight decides with the size of the AD conversion value of three AD converter circuit, if AD conversion value maximum, the conversion of applicable AD converter after the operational amplification circuit gain process on this road in the operational amplification circuit of the second level of current red tide fluorescence signal is described, the weight of this road red tide fluorescence signal so, illustrate that red tide fluorescence signal is the most suitable in the processing of corresponding analog channel, this numerical value is the most accurate, weight selection is n1=9, AD transformation result time large weight is n2=0.8, the effect to this passage of the explanation fluorescence signal of transformation result minimum is poor, and weight minimum is n3=0.2.

When three AD conversion value sizes are: AD1>AD2>AD3, the result that digital processing circuit 30 obtains after processing is so:

$\mathrm{AD}=\frac{k1\×\frac{\mathrm{AD}1}{f1}+k2\×\frac{\mathrm{AD}2}{f2}+k3\×\frac{\mathrm{AD}3}{f3}}{k1+k2+k3}$

When three AD conversion value sizes are: AD1>AD3>AD2, the result that digital processing circuit 30 obtains after processing is so:

$\mathrm{AD}=\frac{k1\×\frac{\mathrm{AD}1}{f1}+k2\×\frac{\mathrm{AD}2}{f2}+k3\×\frac{\mathrm{AD}3}{f3}}{k1+k2+k3}$

When three AD conversion value sizes are: AD2>AD1>AD3, the result that digital processing circuit 30 obtains after processing is so:

$\mathrm{AD}=\frac{k2\×\frac{\mathrm{AD}1}{f1}+k1\×\frac{\mathrm{AD}2}{f2}+k3\×\frac{\mathrm{AD}3}{f3}}{k1+k2+k3}$

When three AD conversion value sizes are: AD2>AD3>AD1, the result that digital processing circuit 30 obtains after processing is so:

$\mathrm{AD}=\frac{k1\×\frac{\mathrm{AD}1}{f1}+k2\×\frac{\mathrm{AD}2}{f2}+k3\×\frac{\mathrm{AD}3}{f3}}{k1+k2+k3}$

When three AD conversion value sizes are: AD3>AD1>AD2, the result that digital processing circuit 30 obtains after processing is so:

$\mathrm{AD}=\frac{k1\×\frac{\mathrm{AD}1}{f1}+k2\×\frac{\mathrm{AD}2}{f2}+k3\×\frac{\mathrm{AD}3}{f3}}{k1+k2+k3}$

When three AD conversion value sizes are: AD3>AD2>AD1, the result that digital processing circuit 30 obtains after processing is so:

$\mathrm{AD}=\frac{k3\×\frac{\mathrm{AD}1}{f1}+k2\×\frac{\mathrm{AD}2}{f2}+k1\×\frac{\mathrm{AD}3}{f3}}{k1+k2+k3}$

Embodiment 2:

Preferably, as shown in Figure 3 A and Figure 3 B, as the further improvement of the testing circuit of realization of the present invention, the phytoplankton fluorescence signal testing circuit second tunnel phytoplankton fluorescence signal testing circuits that increase in embodiment 1, the second described tunnel phytoplankton fluorescence signal testing circuit is made up of analog signal processing circuit, photomultiplier Acquisition Circuit 20 and latch cicuit more.Described analog signal processing circuit is made up of two-stage calculation amplifying circuit and analog and digital signal change-over circuit, first order operational amplification circuit is made up of operational amplification circuit 101 ', and second level operational amplification circuit is by operational amplification circuit 1021 ', operational amplification circuit 1022 ', the parallel formation of operational amplification circuit 1023 '; Described analog and digital signal change-over circuit 112 ' is made up of AD converter circuit 1121 ', AD converter circuit 1122 ', AD converter circuit 1123 '.The connected mode of the second tunnel phytoplankton fluorescence signal testing circuit described in this embodiment is identical with embodiment 1, and the second tunnel phytoplankton fluorescence signal testing circuit and embodiment 1 described in this embodiment use common digital processing circuit 30, reference source circuit 40, crystal oscillating circuit 41 and addressing circuit 42; The circuit of this embodiment can detect two of a phytoplankton wave band fluorescence signal.

The structure of the circuit form of the phytoplankton testing circuit in 2 cited embodiment in above-described embodiment, can also there is above the method for designing of other form, such as the chip of described operational amplification circuit adopts AD8622 chip etc., described AD converter adopts AD1380 chip, and described latch adopts the digital processing circuits 30 such as 74HC563 chip, M54HC573 chip.

It should be noted that, although the present invention has enumerated the testing circuit of above-mentioned 2 embodiment phytoplankton fluorescence signal detection method of the present invention has been described, but the each step based on phytoplankton fluorescence signal detection method of the present invention is to adopt other circuit structures of prior art to realize, and exemplifies no longer one by one explanation in this.

Although specifically show and introduced the present invention in conjunction with preferred embodiment; but those skilled in the art should be understood that; not departing from the spirit and scope of the present invention that appended claims limits; can make a variety of changes the present invention in the form and details, be protection scope of the present invention.

Claims (3)

1. a phytoplankton fluorescence signal detection method, comprises the steps:

A, the step of collection phytoplankton fluorescence signal;

B, the fluorescence signal of the simulation of collection is amplified to the step of processing, specifically: first the fluorescence signal of this simulation is carried out to first order gain and amplify, signal after again this first order being amplified divides that n road is parallel carries out respectively second level gain and amplify, and this n road is parallel carries out the gain that second level gain amplifies and be respectively different f1～fn;

C, the simulating signal after the gain of parallel n road is amplified is carried out analog and digital signal conversion process respectively and converts to the step of digital signal, and the conversion value of the n a changing digital signal is AD1～ADn;

D, the digital signal after n is changed is carried out the step of data processing, specifically: determine different weight size k1～kn according to the numerical values recited of n conversion value AD1～And, thereby testing result is calculated as:

$\mathrm{AD}=\frac{k_1\×\frac{{\mathrm{AD}}_1}{f_1}+k_2\×\frac{{\mathrm{AD}}_2}{f_2}+k_3\×\frac{{\mathrm{AD}}_3}{f_3}...+k_{n-1}\×\frac{{\mathrm{AD}}_{n-1}}{f_{n-1}}+k_n\×\frac{{\mathrm{AD}}_n}{f_n}}{k_1+k_2+k_3+...+k_{n-1}+k_n}.$

2. a kind of phytoplankton fluorescence signal detection method according to claim 1, is characterized in that: after step C, enter and also increase a step C1 before step D: the step that n conversion value AD1～ADn carried out to data latch.

3. a kind of phytoplankton fluorescence signal detection method according to claim 1 and 2, is characterized in that: for detection of the phytoplankton fluorescence signal of m wave band.