CN104178174B - Method for stabilizing and enhancing inoxidability of EGCG (Epigallocatechin Gallate) contained in water-based solution and application thereof - Google Patents
Method for stabilizing and enhancing inoxidability of EGCG (Epigallocatechin Gallate) contained in water-based solution and application thereof Download PDFInfo
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Abstract
The invention discloses a method for stabilizing and enhancing the inoxidability of EGCG (Epigallocatechin Gallate) contained in a water-based solution and application thereof. The method is capable of stabilizing and enhancing the inoxidability of the EGCG contained in the water-based solution by using Cu<2+> and EGCG together, wherein Cu<2+> is from a matrix or an inorganic bivalent copper ion. The method disclosed by the invention can be applied to the inoxidability improvement of such water-based solutions as beverage and functional beverage, such daily chemicals as water-based cosmetic products and washing products, water-soluble coating and such fertilizers as water-based fertilizers; the method is simple to operate and consumes less raw material; the process of the method can be used together with other processes in a production process; and no special equipment is needed, so that the cost is greatly saved, and the method is easy to use.
Description
Technical field
The present invention relates to stabilize and increase EGCG non-oxidizability method in aqueous solution.More particularly it relates to
To Cu2+, its source is medium or inorganic bivalent cupric ion, is used in mixed way with EGCG, can stabilize and increase in aqueous solution
The method of EGCG non-oxidizability, is applied to EGCG as the water-based beverage of additive, daily chemical product, water paint, water
The non-oxidizability improvement of the products such as molten fertilizer.
Background technology
Epigallocatechin gallate (EGCG) (EGCG) is important antioxidant in tea extract, and antioxidant activity is
Ascorbic 100 times, 25 times of vitamin E, cell can be protected, it is to avoid cancer, heart disease and other great diseases simultaneously
Sick.Effect of EGCG comes from the removing to oxygen-derived free radicals (antioxidation) ability, is widely used in food, medicine, nutrition at present
In the product such as product and daily-use chemical industry.As food additive application in fruits and vegetables is fresh-keeping, bacterial reproduction can be suppressed, protect
Hold veterinary antibiotics color originally, reach the purpose of preservation and antisepsis, reduce vitamin C (VC) loss.It is used for preparing various tea-drinking
Material, can suppress the multivitamin destructions such as vitamin A in fruit drink (VA) and VC, have resisting fatigue and antioxygen is turned into
With, and the effect of slow down aging, and contribute to improving mouthfeel.EGCG has sun-proof, radiation proof function, in addition antioxidation simultaneously
Property, it is used for cosmetics and other household chemicalss.
EGCG itself is the most unstable, and easily oxidation or polymerization, produce EGCG polymer and hydrogen peroxide,
Make its antioxygenic property unstable, bring difficulty to application.To EGCG application in terms of oils and fats, make fat-soluble modification, including
Methyl, ethyl or acetylation become EGCG, become the focus of attention.On the contrary, utilize for water solublity splendid for EGCG, be not subject to
To enough attention.
Summary of the invention
The invention discloses one stabilize and increase epigallocatechin gallate (EGCG) in aqueous solution (EGCG) resist
The method of oxidisability and application.The present invention relates to Cu2+, its source is medium or inorganic bivalent cupric ion, mixes with EGCG and makes
With, the method for EGCG non-oxidizability in aqueous solution can be stabilized and increased, be applied to drink as the aqueous of additive with EGCG
The non-oxidizability improvement of the products such as material, daily chemical product, water paint, water-soluble fertilizer.
The technical solution used in the present invention is for achieving the above object:
A kind of stabilize and increase the side of epigallocatechin gallate (EGCG) EGCG non-oxidizability in aqueous solution system
Method, it is characterised in that: utilize substrate Cu2+Concentration also suitably adjusts, and makes Cu in aqueous solution system2+Concentration is at 50 ~ 250 μ
mol·L-1In, it is 6.8 ~ 7.3 that system pH controls, and temperature is 15～25 DEG C, adds EGCG solid or highly concentrated solution, controls close
Close container mixed solution EGCG and Cu2+Whole molar concentration rate be (2～3): 1, gentle agitation mix homogeneously.
Specifically include following steps:
(1), the copper ion concentration in aqueous solution raw material and pH value are measured；
(2), regulating pH=6.8～7.3 in aqueous solution raw material, this pH value should meet production and quality standard simultaneously
Requirement；
(3), according to the measurement result of copper ion concentration, Cu2+ concentration is adjusted at 50～250 μm ol L-1Scope, if
Cu2+Concentration is low, and the available copper chloride meeting production requirement or other Inorganic Copper ions add Cu2+, meet the requirements model to concentration
Enclose, and product itself meets its production, safety and quality standard, if Cu2+Concentration is high, can require to add according to production and permit
The solution permitted is diluted, and product itself meets its production, safety and quality standard, the Cu after adjustment2+Concentration range should accord with
Close production and quality standard；
(4), step (3) is adjusted Cu2+The aqueous solution of concentration transfers to airtight container, is then added to
EGCG solution, it is desirable to EGCG and Cu in final solution2+Molar concentration rate be 2:1 or 3:1, add after EGCG, slight concussion or
Stirring, mix homogeneously, get final product discharging.
Described stabilizes and increases the method for epigallocatechin gallate (EGCG) EGCG non-oxidizability in aqueous solution,
It is characterized in that, Cu in described mensuration aqueous solution2+The concrete grammar of concentration: survey with reference to the method for GBT 8538-2008
Fixed, take sample, can directly measure if sample is settled solution, if sample is float or the high sample of the content of organic matter
Product, need to first filter, and collect filtrate, and filtrate is acidified with 1.5ml nitric acid/1000L after 0.45 μm film, filtrate, and 5ml nitric acid/
After 1000L digestion, measure copper ion concentration in substrate referring next to GBT 8538-2008 Copper Ion method.
Described one stabilizes and increases epigallocatechin gallate (EGCG) EGCG antioxidation in aqueous solution system
Property the application of method, it is characterised in that: the method is applicable to produce as water-based beverage, the daily-use chemical industry of additive with EGCG
The non-oxidizability improvement aspect of the products such as product, water paint, water-soluble fertilizer
By substrate (aqueous solution system) contained substance own, (present invention refers to the Cu in substrate2+), stabilize and increase
During EGCG uses, non-oxidizability formula is extremely rare.The present invention utilizes existing Cu in substrate2+, it is adjusted to invention institute
State concentration range, then apply EGCG on request.The present invention is simple, can greatly improve the stability of EGCG, expands EGCG
Range of application；Improve the non-oxidizability of EGCG in application, reduce cost；In formula, material can be derived partly from substrate simultaneously
In, implementation process reduces artificial interpolation, more environmentally friendly；EGCG originates edible natural plant Folium Camelliae sinensis, therefore can be in food, dailyization
Apply on the Related products such as product, coating and fertilizer, more green safety.
Patent correlation test concrete operation step and test result analysis: the present invention relates to stabilize and increase EGCG
The formula of non-oxidizability and method in aqueous solution.
The method includes that the detection of formula material, formula proportion, stroma ground substance, application conditions, formula material add, mix
Deng.Method according to substrate internal substance by regulation after, add a certain proportion of EGCG, stabilize and increase the antioxygen of EGCG
The property changed, formula and method can reach good application effect.
(1), Cu in substrate (aqueous solution)2+Content detection: Cu in detection substrate (aqueous solution)2+Content method ginseng
Examining GBT 8538-2008, take solution, settled solution can directly measure, float or the high sample of the content of organic matter, needed
Filter, crosses 0.45 μm film, and with 1.5ml nitric acid/1000L acidifying, 5ml nitric acid/1000L digestion, referring next to GBT 8538-
2008 Copper Ion methods measure copper ion in substrate；
(2), regulation pH: measure and regulate pH, making solution system pH=6.8～7.3；It is raw that this pH value should meet product simultaneously
Produce and quality criteria requirements
(3), Cu is adjusted2+Concentration: according to testing result, by adding deionized water or adding Cu2+Adjust Cu2+Concentration
At 50 ~ 250 μm ol L-1Scope, if desired for add Cu2+, available copper chloride or other Inorganic Coppers meeting production requirement
Ion, the Cu after adjustment2+Concentration range should meet production and quality standard.
(4), EGCG and Cu2+Proportioning.With EGCG and Cu2+Concentration is than for 3:1(C EGCG:C Cu 2+=3:1), add EGCG.Ratio
Example the most preferably less than 2:1.
(5), EGCG preparation.EGCG purity reaches 98.0%, is dissolved into concentration with deionized water and is at least 1000 μm ol
L-1, it is to avoid it is stirred vigorously；Or the amount of calculating EGCG solid, make EGCG add final concentration according to formula and meet the requirement in " 4 ".
(6), adding method.Reaction vessel requires normal pressure, airtight, reduces air as far as possible and get involved after adding EGCG, can
Small product size is utilized to account for container volume to reduce air contact.After adding formula material, slight concussion or stirring, can not acutely shake
Swing or stir, mix homogeneously.
Beneficial effects of the present invention:
1, the stability during EGCG uses and anti-oxidation function are improved.
2, range is wide.Go for without organic aqueous solution, such as water-based beverage.Equally applicable containing organic
The non-oxidizability improvement of the products such as the water-based beverage of matter, daily chemical product, water paint, water-soluble fertilizer.
3, Medium Culture inclusion is utilized, it is to avoid repeat metering, cost-effective, reduce resource and use.
4, method is extremely simple, does not has especial equipment requirements.
5, EGCG source edible natural is with plant, has wide range of applications, meets environmental requirement.
Accompanying drawing explanation
Fig. 1 is with assay NBT photoreduction, detects antioxidative stabilizer result by EGCG parallel sample application condition,
(n=6) (in figureC Cu 2+=100μmol·L-1).
Fig. 2 is that the comparison diagram that detection antioxygenic property improves is (in figure with assay NBT photoreductionC Cu 2+=100μmol·
L-1).
Detailed description of the invention
Application claims Cu2+Meet target level of product quality scope.
A kind of stabilize and increase the side of epigallocatechin gallate (EGCG) EGCG non-oxidizability in aqueous solution system
Method, comprises the following steps:
(1), the copper ion concentration in aqueous solution raw material and pH value are measured；
(2), regulating pH=6.8～7.3 in aqueous solution raw material, this pH value should meet production and quality standard simultaneously
Requirement；
(3), according to the measurement result of copper ion concentration, Cu is adjusted2+Concentration is at 50～250 μm ol L-1Scope.If
Cu2+Concentration is low, and the available copper chloride meeting production requirement or other Inorganic Copper ions add Cu2+, meet the requirements model to concentration
Enclose, and product itself meets its production, safety and quality standard；If Cu2+Concentration is high, can require to add according to production and permit
The solution permitted is diluted, and product itself meets its production, safety and quality standard.Cu after adjustment2+Concentration range should accord with
Close production and quality standard；
(4), step (3) is adjusted Cu2+The aqueous solution of concentration transfers to airtight container, is then added to
EGCG solution, it is desirable to EGCG and Cu in final solution2+Molar concentration rate be 2:1 or 3:1, add after EGCG, slightly shake
Or stirring, mix homogeneously, get final product discharging.
With assay NBT photoreduction, detect antioxidative stabilizer result by EGCG parallel sample application condition.CCu 2+=
Antioxidant effect during 100 μm ol L-1, result is shown in Fig. 1.Testing result is to be not added with the EGCG sample non-oxidizability of Cu2+ by mistake
Difference reaches ± 0.29, according to CEGCG: CCu 2+ =3:1 adds Cu2+Sample non-oxidizability error only ± 0.025, by CEGCG: CCu 2+
The ratio of=2:1, adds Cu2+Sample non-oxidizability error be ± 0.010.Visible by formula substance after, system antioxidation
Stability significantly improves.
Fig. 2 is with assay NBT photoreduction, detects the result figure that antioxygenic property improves,C Cu 2+=100μmol·L-1
Time antioxidant effect, CEGCG: CCu 2+ The system of=3:1 is relative to isoconcentration Cu2+System, antioxygenic property improves to 300%；Relatively
In without Cu2+With the system of EGCG, non-oxidizability improves to 120%.Visible by formula substance after, system antioxygenic property
Also significantly improve.
Claims (2)
1. stabilize and increase a method for epigallocatechin gallate (EGCG) EGCG non-oxidizability in aqueous solution system,
It is characterized in that: utilize substrate Cu2+Concentration also suitably adjusts, and makes Cu in aqueous solution system2+Concentration is at 50 ~ 250 μm ol L-1
In, system pH control be 6.8 ~ 7.3, temperature is 15～25 DEG C, add EGCG solution, control hermetic container mixed solution EGCG and
Cu2+Whole molar concentration rate be 2:1 or 3:1, gentle agitation mix homogeneously；
Described method specifically includes following steps:
(1) copper ion concentration in aqueous solution raw material and pH value are measured；
(2) pH=6.8～7.3 in regulation aqueous solution raw material, this pH value should meet production and quality criteria requirements simultaneously；
(3) according to the measurement result of copper ion concentration, Cu is adjusted2+Concentration is at 50～250 μm ol L-1Scope, if Cu2+
Concentration is low, utilizes the copper chloride meeting production requirement to add Cu2+, meet the requirements scope to concentration, and product itself to meet it raw
Product, safety and quality standard, if Cu2+Concentration is high, requires that adding the solution allowed is diluted according to production, and product
Itself meet its production, safety and quality standard, the Cu after adjustment2+Concentration range should meet production and quality standard；
(4) step (3) is adjusted Cu2+The aqueous solution of concentration transfers to airtight container, is then added to EGCG molten
Liquid, it is desirable to EGCG and Cu in final solution2+Molar concentration rate be 2:1 or 3:1, add after EGCG, slight concussion or stirring,
Mix homogeneously, gets final product discharging；
Cu in described mensuration aqueous solution2+The concrete grammar of concentration: measure with reference to the method for GBT 8538-2008, sampling
Product, can directly measure if sample is settled solution, if sample is float or the high sample of the content of organic matter, need the thickest
Filtering, collect filtrate, filtrate is after 0.45 μm film, and filtrate is with 1.5ml nitric acid/1000L acidifying, 5ml nitric acid/1000L nitre
After change, measure copper ion concentration in substrate referring next to GBT 8538-2008 Copper Ion method.
2. epigallocatechin gallate (EGCG) EGCG during the one described in claim 1 stabilizes and increases aqueous solution system
The application of the method for non-oxidizability, it is characterised in that: the method is applicable to EGCG as the water-based beverage of additive, dailyization
The non-oxidizability improvement aspect of chemical product.
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| CN100361655C (en) * | 2005-09-05 | 2008-01-16 | 中山大学 | (-)-epigallocatechin gallate-calcium complex solid dispersion and its prepn and application |
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