CN104151222A

CN104151222A - Method for extracting allicin

Info

Publication number: CN104151222A
Application number: CN201410341579.8A
Authority: CN
Inventors: 赵远
Original assignee: Changzhou University
Current assignee: Changzhou University
Priority date: 2014-07-18
Filing date: 2014-07-18
Publication date: 2014-11-19

Abstract

The invention relates to a method for extracting allicin, belonging to the field of bio-pharmaceuticals. The method comprises the following steps: soaking garlic for 30 minutes, peeling off, removing foreign matters and decayed garlic, washing with ultrasonic waves, pounding the washed garlic into paste, putting the paste into a drying oven for drying for a certain time, grinding into powder in a crushing machine, screening, and adding cellulose and sodium aliphatate respectively for hydrolysis; soaking in a mixed solution for extraction; adding a petroleum ether solution for soaking the hydrolyzed substance; and transferring the separated leachate on an upper layer into another Soxhlet extraction bottle for extraction, and concentrating, thereby obtaining allicin. The method can be executed at normal temperature and normal pressure, no CO2 supercritical pressure or temperature is needed, the energy consumption is reduced, and meanwhile the operation is relatively simple.

Description

A kind of extracting method of garlicin

Technical field

The extracting method that the present invention relates to a kind of garlicin, belongs to field of biological pharmacy.

Background technology

Garlic originates in south of europe and western part of Asia the earliest, at B.C. 116 years, stuttering is incorporated into Hanzhong Area of Shaanxi from the Western Regions, progressively spreads all over afterwards the whole nation.16th century, frontal lobe also started cultivation, at present extend over the entire globe in North America.The chemical composition more complicated of garlic, of a great variety, also very complete, wherein contain abundant active sulfide, be of value to general cardiovascular protection.In Bulbus Allii quintessence oil, contain the natural active matter of about 140 kinds, mainly can be divided into two classes: volatile component and involatile constituent, a large amount of medical experiments show, Bulbus Allii quintessence oil has antibacterial killing the virus, reducing blood-fat, prevention of arterial sclerosis, hypoglycemic, the effects such as lowering blood-fat and reducing weight.

The chemical composition of garlic and complexity thereof, but garlicin is the main ingredient in Bulbus Allii extract.Mechanism research shows, Bulbus Allii quintessence oil is to form under the effect of allinase, and its reaction substrate is a kind of non-protein amino acid-alliin class material in scallion garlic type crop, and chemical composition is alkylthio hemiamic acid and sub-pendant compounds thereof.The extracting method of traditional Bulbus Allii quintessence oil has steam distillation, extraction extraction process, allow etc. with supercritical carbon dioxide extraction method, but all there is defect in various degree in this several method, extract length consuming time, essential oil quality is unstable, mostly exists Allicin Content lower,, low, the color and luster of productive rate, weak flavor or dissolvent residual etc.

Summary of the invention

The present invention is directed to and in traditional method, extract length consuming time, essential oil quality is unstable, operational management is difficult, can not widespread use etc. problem, proposed a kind ofly under normal temperature, normal pressure, can carry out, without CO ₂the extracting method of a kind of garlicin of supercritical pressure and temperature makes operation become simpler in energy efficient.

For achieving the above object, the concrete technical scheme that the present invention takes is:

(1) garlic is soaked in water 30 min, peeling, rejects foreign matter and rotten garlic, under hyperacoustic effect, washs 5～10min;

(2) garlic after cleaning is pulverized into pasty state, put into baking oven and dry 6～8 hours, oven temperature is controlled at 60～65 DEG C;

(3) wear into powder with pulverizer, cross 80～100 mesh sieves, add respectively 10～20g cellulase and the hydrolysis of 100～200g sodium soap; Individual hour of extracting 10-12 in immersion;

(4) in the above-mentioned substance after hydrolysis, add mass concentration be 25%-35% petroleum ether solution soak 10～12 hours;

(5) isolated upper strata leaching liquid is proceeded in another Soxhlet extracting bottle to extracting after 3～6 hours, through concentrating and obtain garlicin.

innovative point:

(1) the present invention can carry out under normal temperature, normal pressure, without CO ₂supercritical pressure and temperature make operation become simpler in energy efficient.

Embodiment

By garlic 30 min that are soaked in water, peeling, rejects foreign matter and rotten garlic, under hyperacoustic effect, washs 5～10min; Garlic after cleaning is pulverized into pasty state, put into baking oven and dry 6～8 hours, oven temperature is controlled at 60～65 DEG C; Wear into powder with pulverizer, cross 80～100 mesh sieves, add respectively 10～20g cellulase and the hydrolysis of 100～200g sodium soap; Extracting 10～12 hours in immersion; In above-mentioned substance after hydrolysis, add mass concentration be 25%-35% petroleum ether solution soak 10～12 hours; Isolated upper strata leaching liquid was proceeded in another Soxhlet extracting bottle to extracting after 3～6 hours, through concentrating and obtain garlicin.

Example 1

Get 100g garlic 30 min that are soaked in water, peeling, rejects foreign matter and rotten garlic, under hyperacoustic effect, washs 5min; Garlic after cleaning is pulverized into pasty state, put into baking oven and dry 6 hours, oven temperature is controlled at 60 DEG C; Wear into powder with pulverizer, cross 80 mesh sieves, add respectively 10g cellulase and the hydrolysis of 100g sodium soap; Extracting 10 hours in immersion; In above-mentioned substance after hydrolysis, adding mass concentration is that 25% petroleum ether solution soaks 10 hours; Isolated upper strata leaching liquid is proceeded to extracting in another Soxhlet extracting bottle and, after 3 hours, obtain 120g garlicin through concentrating.

Example 2

Get 100g garlic 30 min that are soaked in water, peeling, rejects foreign matter and rotten garlic, under hyperacoustic effect, washs 7min; Garlic after cleaning is pulverized into pasty state, put into baking oven and dry 7 hours, oven temperature is controlled at 63 DEG C; Wear into powder with pulverizer, cross 90 mesh sieves, add respectively 15g cellulase and the hydrolysis of 150g sodium soap; Extracting 11 hours in immersion; In above-mentioned substance after hydrolysis, adding mass concentration is that 30% petroleum ether solution soaks 11 hours; Isolated upper strata leaching liquid is proceeded to extracting in another Soxhlet extracting bottle and, after 4 hours, obtain 130g garlicin through concentrating.

Example 3

Get 100g garlic 30 min that are soaked in water, peeling, rejects foreign matter and rotten garlic, under hyperacoustic effect, washs 10min; Garlic after cleaning is pulverized into pasty state, put into baking oven and dry 8 hours, oven temperature is controlled at 65 DEG C; Wear into powder with pulverizer, cross 100 mesh sieves, add respectively 20g cellulase and the hydrolysis of 200g sodium soap; Extracting 12 hours in immersion; In above-mentioned substance after hydrolysis, adding mass concentration is that 35% petroleum ether solution soaks 12 hours; Isolated upper strata leaching liquid is proceeded to extracting in another Soxhlet extracting bottle and, after 6 hours, obtain 140g garlicin through concentrating.

Claims

1. an extracting method for garlicin, is characterized in that:

(3) wear into powder with pulverizer, cross 80～100 mesh sieves, add respectively 10～20g cellulase and the hydrolysis of 100～200g sodium soap;