CN104147081A

CN104147081A - Blueberry lutein soft capsule for improving eyesight and manufacturing method thereof

Info

Publication number: CN104147081A
Application number: CN201410412592.8A
Authority: CN
Inventors: 孙桂芳
Original assignee: HARBIN SHUNYITANG MEDICINE BIOTECHNOLOGY DEVELOPMENT Co Ltd
Current assignee: HARBIN SHUNYITANG MEDICINE BIOTECHNOLOGY DEVELOPMENT Co Ltd
Priority date: 2014-08-20
Filing date: 2014-08-20
Publication date: 2014-11-19

Abstract

The invention discloses a blueberry lutein soft capsule for improving eyesight and a manufacturing method thereof. The blueberry lutein is a nutrition element, which is good for improving eyesight and can reach the eye ground. The proper supply of the blueberry lutein is good for improving eyesight, but excessive blueberry lutein is not good. The blueberry lutein soft capsule is composed of a capsule shell and a content, wherein the content is stored in the capsule shell; the content is composed of the following components in parts by weight: 43 to 47 parts of blueberry extract, 20 to 23 parts of zinc gluconate, 14 to 16 parts of vitamin C, 11 to 14 parts of lutein powder, 3 to 4 parts of vitamin E, 0.2 to 1 part of vitamin A, 143 to 146 parts of soybean oil, and 6 to 9 parts of bee wax; and the capsule shell is composed of the following components in parts by weight: 190 to 210 parts of gelatin, 70 to 90 parts of glycerin, 190 to 210 parts of purified water, and 9 to 10 parts of caramel color. The blueberry lutein soft capsule is used to improve eyesight.

Description

Improve blue berry xanthophyll soft capsule and the manufacture method of vision

technical field:

the present invention relates to a kind of blue berry xanthophyll soft capsule and manufacture method of improving vision.

background technology:

the Blue berry leaf flavin that at present medical circle is found can directly arrive the vision nutrient on optical fundus, although phylloxanthin can improve vision, protection eyes, anything is not The more the better, supply appropriate just can, eat unhelpful more.Can supplement by appropriate, convenient oral, the capsule of the Blue berry leaf flavin of change vision is urgently to be resolved hurrily,

blue berry leaf flavin, from plant, extract, in eye, content is more, and human body can not oneself synthesize, so, enough her molecule phylloxanthins of taking are all beneficial to eyes at ordinary times, still, and when people take conventionally, all to take by capsule, although because the content of beary metal of capsule shells has strict regulation, how much still have content overproof, the taking of long period has an Accumulation of heavy metals problem and easily causes health harm.

summary of the invention:

the object of this invention is to provide a kind of blue berry xanthophyll soft capsule and manufacture method of improving vision.

above-mentioned object realizes by following technical scheme:

a kind of blue berry xanthophyll soft capsule that improves vision, its composition comprises: capsule shells, in described capsule shells, have content, described content comprises that parts by weight are the Pericarpium Citri tangerinae extract of 43-47, the zinc gluconate that parts by weight are 20-23, the vitamin C that parts by weight are 14-16, the phylloxanthin powder that parts by weight are 11-14, the vitamin E that parts by weight are 3-4, the vitamin A that parts by weight are 0.2-1, the soybean oil that parts by weight are 143-146, the peak wax that parts by weight are 6-9; Described capsule shells comprises that parts by weight are the gelatin of 190-210, the glycerol that parts by weight are 70-90, the purified water that parts by weight are 190-210, the burnt sugar coloring that parts by weight are 9-10.

the described blue berry xanthophyll soft capsule that improves vision, described content comprises that parts by weight are 43 Pericarpium Citri tangerinae extract, the zinc gluconate that parts by weight are 20, the vitamin C that parts by weight are 14, the phylloxanthin powder that parts by weight are 11, the vitamin E that parts by weight are 3, the vitamin A that parts by weight are 0.2, the soybean oil that parts by weight are 143, the peak wax that parts by weight are 6; Described capsule shells comprises that parts by weight are 190 gelatin, the glycerol that parts by weight are 70, the purified water that parts by weight are 190, the burnt sugar coloring that parts by weight are 9.

the described blue berry xanthophyll soft capsule that improves vision, described content comprises that parts by weight are 47 Pericarpium Citri tangerinae extract, the zinc gluconate that parts by weight are 23, the vitamin C that parts by weight are 16, the phylloxanthin powder that parts by weight are 14, the vitamin E that parts by weight are 4, the vitamin A that parts by weight are 1, the soybean oil that parts by weight are 146, the peak wax that parts by weight are 9; Described capsule shells comprises that parts by weight are 210 gelatin, the glycerol that parts by weight are 90, the purified water that parts by weight are 210, the burnt sugar coloring that parts by weight are 10.

the described blue berry xanthophyll soft capsule that improves vision, described content comprises that parts by weight are 45 Pericarpium Citri tangerinae extract, the zinc gluconate that parts by weight are 21.5, the vitamin C that parts by weight are 15, the phylloxanthin powder that parts by weight are 12.5, the vitamin E that parts by weight are 3.3, the vitamin A that parts by weight are 0.6, the soybean oil that parts by weight are 144.5, the peak wax that parts by weight are 7.6; Described capsule shells comprises that parts by weight are 200 gelatin, the glycerol that parts by weight are 80, the purified water that parts by weight are 200, the burnt sugar coloring that parts by weight are 9.6.

a manufacture method of improving the blue berry xanthophyll soft capsule of vision, the method comprises the steps:

(1) processing of raw material: sieve, weigh: Pericarpium Citri tangerinae extract, zinc gluconate, vitamin C, phylloxanthin powder are crossed respectively to 80 mesh sieves, according to formula for a product, calculate the inventory of each supplementary material, then accurately weigh, standby;

(2) batching: by Pericarpium Citri tangerinae extract, zinc gluconate, vitamin C, phylloxanthin powder mix homogeneously, about 20min, obtains mixture; When soybean oil is heated to 75 ℃, add Cera Flava, stir melting, about 1h, obtains oily wax liquid, is cooled to 50 ℃ of insulations, stand-by; VitAVitE, mixture are added in oily wax liquid successively, and limit edged stirs, and about 30min crosses colloid mill 2 ~ 3 times after mix homogeneously, and the de-bubble to the greatest extent of-0.06 ~-0.08Mpa, obtains content stand-by;

(3) change glue: first will in purified water, putting into of glycerol glue tank, be heated to 70 ℃, under stirring, gelatin, burnt sugar coloring are slowly added, and continue to be heated to 80 ℃, stirring boils 1h, de-bubbled-0.06 ~-0.08 Mpa, 65 ~ 75 ℃, obtain glue, and 80 orders filter, at 50 ℃, place 4 ~ 6h, standby;

(4) pelleting: start soft capsule press, compacting soft gelatin capsule.18 ~ 26 ℃ of production environment temperature, relative humidity 30 ~ 40%, controls loading amount 0.25g/ grain;

(5) sizing: the soft gelatin capsule suppressing is shaped in rotating cage with tape transport.18 ~ 26 ℃ of temperature, relative humidity 30 ~ 40%, about 4h of time;

(6) wash ball, dry: with 95% edible ethanol, clean soft gelatin capsule, until soft gelatin capsule any surface finish, bright, without grease, is then sent into soft gelatin capsule hothouse dry, control 24 ~ 30 ℃ of hothouse temperature, relative humidity 20 ~ 30%, dry about 24h;

(7) lamp is picked up: dried soft capsule is placed on lamp inspection machine, special messenger picks, conscientiously check soft capsule presentation quality, by empty pocket, special-shaped ball, flat ball, thin-walled ball, not of uniform sizely cause that ball, surface are untidy, bonding, distortion, leak the defective works such as capsule and sort out;

(8) pressing plate: soft capsule is carried out to pressing plate, 10/plate, adopt the hard sheet of polrvinyl chloride solid medicinal.

beneficial effect:

1. the present invention can make excess eye-using, visual function decline long period of operation computer, contact electromagnetic radiation person's the personage can life-time service; can vision protection; alleviate eyestrain; to visual function descender, myope, wish, prevent the personage of senile glaucoma, cataract patient, the caused patient with retinopathy of diabetes to take; can make symptom alleviate, effect is remarkable.Can absorb ultraviolet and blue light, avoid the damage of harmful light to eyes, and can repair eyes photic damage, delay eyes aging and prevent pathological changes.(protection, renovation agent) prevents physiological structure and the function variation of eyes, is a kind of vision nutrient.(nutrient); There is vasodilation function simultaneously, improve optical fundus blood microcirculation, promote nutrient substance supply (dredging agent); Can promote generating of rhodopsin in retina cell, prevention high myopia and detachment of retina, promote vision, vision protection.

2. the present invention is according to the check of Ministry of Public Health < < health food and assessment technique standard > > (version in 2003), this health food has been carried out scheduling to last to the effect assessment of the human feeding trial of 35d, result shows: test group right and left eyes distant vision is all than making moderate progress before test-meal, and difference has statistical significance (P<0.05); Comparing difference not statistically significant (P>0.05) before right and left eyes distant vision and test-meal after matched group test-meal.After test-meal, test group duration of photopic vision has improved 0.12 ± 0.13, and after test-meal, test group duration of photopic vision and matched group compare difference statistical significance (P<0.01); The eye symptom integration of test group is reduced to 2.25 ± 1.86 after taking by taking 3.92 ± 1.94 before tested material, and front and back comparing difference has statistical significance (P<0.01); After test-meal, test group eye symptom integration and matched group (3.35 ± 1.85) comparing difference has statistical significance (P<0.01).Test group total effective rate (49.02%) higher than (3.92%) of matched group (P<0.05).Conclusion: test confirms that this health food has the effect of asthenopia releasing function to human body.

3. Blue berry leaf flavin capsule of the present invention has antioxidative effect.The antioxidation that phylloxanthin is stronger; the activity that can suppress reactive oxygen free radical; stop reactive oxygen free radical to Normocellular destruction, experiment showed, that reactive oxygen free radical can react with DNA, protein, lipid; weaken their physiological function; and then cause such as cancer, arteriosclerosis, increasing the generation of the yellow magic degeneration disease chronic diseases of rheological properties, phylloxanthin can pass through physics or chemical cancellation effect deactivation singlet oxygen; thereby protection body escapes injury, the immunocompetence of enhancing body.

4. the Pericarpium Citri tangerinae extract in the present invention is the procyanidin that extracts from Pericarpium Citri tangerinae, and procyanidin can improve vision, it is dizzy to alleviate; Can protect blood capillary, promote to look regeneration of erythrocytes, strengthen dark adaptive capacity; Can improve noctovision, alleviate visual fatigue, improve the adaptive capacity of low-light level.Procyanidin function of relieving visual fatigue mechanism is mainly that procyanidin acts on retinal isomerase, increased rhodopsin generating rate, rhodopsin is the photoactive substance of retina optic cell, when rhodopsin is subject to light to amphiblestroid stimulation, can decompose in moment, and this chemical change is sent to brain, thereby generation " visible ", rhodopsin can natural resynthesis when dark place, procyanidin can promote rhodopsin resynthesis in the dark, improves the susceptibility of retina to light.

5. in zinc gluconate of the present invention, zinc is the trace element of needed by human, the phenomenons such as zinc deficiency can make that people occurs that dark adaptation ability weakens, visual deterioration, myopia, hypermetropia and astigmatism.It is connected closely with child's growth promoter, immunologic function, vision and sexual development, and zinc supplement can promote the normal development of children's torso and intelligence; Can strengthen the sensitivity of optic nerve, zinc deficiency can affect vitamin A absorption in vivo.The relation of zinc and vision, is mainly by zinc enzyme (alcoholdehydrogenase), vitamin A (retinol) to be oxidized to optically active substance in rod cell to form retinal, is convenient to form visual pigment, in order to improving Infravision.

6. vitamin C of the present invention is one of composition forming eyeball crystalline lens, and in human eye, ascorbic content is than exceeding 30 times in blood, and with age, Vitamin C content obviously declines, and crystalline lens malnutrition can cause crystalline lens degeneration in the course of time; Vitamin C can weaken the oxygen of light to lenticular infringement, has the effect that prevents that old cataract from forming.According to doctor diagnosed, the ultraviolet in light can form the injury of radical pair tissue, at eyes, the most often causes cataract and old maculopathy, and these two kinds of diseases finally all may cause blind.Prevention, can do from improving the extracted amount of vegetable, especially contains vitamin C and E and carotene, can significantly reduce these diseases.Vitamin C is the important nutritional labeling of eye lens.In addition, vitamin C is also antioxidant, can remove eye free radical, eliminates eye tired.

7. phylloxanthin of the present invention has another name called " plant progesterone ", at occurring in nature and zeaxanthin, jointly exists.It is the key component that forms the plant pigment such as Semen Maydis, vegetable, fruit, flowers; be contained in the chloroplast of leaf; the luminous energy of absorption can be passed to chlorophyll a, infer photooxidation, photo damage are had to protective effect, be also the main pigment that forms human eye retina's macular region.Phylloxanthin be present in human body eye institute in a organized way in, comprise iris tissue, crystalline lens, retina, optic nerve and eye fat, wherein the highest with macula retinae district concentration.Macular area has been concentrated a large amount of cone cells, is the sharpest region of vision, keeps macula lutea health most important for keeping normal vision function.And human body can not synthesize phylloxanthin, can only supplement by taking in.Many Epidemiological study results demonstrations, there is positive correlation in the intake of phylloxanthin and macula retinae pigment density, and a certain amount of phylloxanthin of daily iron supplement can make vision make moderate progress.The mechanism of phylloxanthin vision protection mainly contains optical barrier effect and antioxidation 2 points.Phylloxanthin can absorb a large amount of blue lights, and the wavelength of blue visible light and ultraviolet light approach, and are a kind of light that can reach potential hazard maximum in amphiblestroid visible ray.Before light arrives cell responsive on retina, first pass through the highly the densest district of phylloxanthin, if the lutein content of macula lutea is abundant, just this injury can be reduced to minimum.In addition, phylloxanthin is a kind of stronger antioxidant, has the effect of removing free radical and free radical, can suppress the oxidative damage of the tissues such as retina that many reasons causes, crystalline lens, macula lutea, to safeguard twenty-twenty vision.

8. vitamin E of the present invention is ophthalmology, department of eye conventional medicine clinically, can obviously suppress the lipid peroxidation of the pathological corneas of blister sore toxicity keratitis, shortens pathological changes healing time, reduces complication and sequela.Vitamin E still can suppress the formation of cicatrix, promotes corneal wound healing.Glycosaminoglycan (GAG) exists with the form of protein polysaccharide. and be one of important extracellular matrix.The extracellular matrix of trabecular tissue can regulate the transhipment of the inside and outside material of little fine strain of millet histiocyte and aqueous humor to discharge, it is relevant with the metabolism of GAG in little fine strain of millet tissue that the intraocular pressure of primary open angle glaucoma raises. and recent result of study shows, vitamin E can increase the total amount of the little fine strain of millet histiocyte of the human eye glycosaminoglycan of In vitro culture, increase hyaluronic constituent ratio simultaneously, on pathogenesis, have in the probability normal eyes retina of the open angle glaucoma of preventing and treating and contain abundant antioxidase as superoxide dismutase, glutathion peroxidase etc. and low molecular antioxidant etc., this plays an important role to the normal visual function of retina.

9. vitamin A of the present invention is the nutrient of needed by human, is also the main component of visual pigment, and it has important effect to maintaining normal vision.When vitamin A deficiency or deficiency, can cause the reduction of dark adaptation ability, nyctalopia, cornea keratinization and visual disorder.Modern ophthalmology medical science thinks, when human body is deficient in vitamin A, can affect the synthetic of rhodopsin in eyeball, in eye muscle body, when rhodopsin approach exhaustion or wretched insufficiency, visual deterioration, blurred vision, nyctalopia phenomenon will occur.Because of the physiological action of vitamin A deficiency relevant with the scotopic vision of following, it can synthesize a kind of material that is rhodopsin in eyes, rhodopsin is to keeping normal good visual accommodation to play an important role, and relevant with the structural integrity and the enhancing development that maintain epithelial tissue.

10. of the present invention by gelatin, glycerol, pure water, the synthetic capsule shells of red yeast rice, belong to natural green

capsule, wherein gelatin is a kind of using by the animal bone boiling, the peptide molecule polymeric material that skin and muscle tendon are made, it is the hydrolyzate of collagenic protein, contain a large amount of aminoacid, have higher nutritive value, red yeast rice is exactly that Monas cuspurpureus Went grinds later powder, so because have higher color valency to be widely used in food production.Monascus is inoculated on the rice steaming, cultivation a period of time just can obtain Monas cuspurpureus Went, and Monas cuspurpureus Went is the natural fermented product of microorganism, and human body is safe from harm.

accompanying drawing explanation:

accompanying drawing 1 is production technological process of the present invention.

the specific embodiment:

embodiment 1:

embodiment 2:

according to the blue berry xanthophyll soft capsule that improves vision described in embodiment 1, described content comprises that parts by weight are 43 Pericarpium Citri tangerinae extract, the zinc gluconate that parts by weight are 20, the vitamin C that parts by weight are 14, the phylloxanthin powder that parts by weight are 11, the vitamin E that parts by weight are 3, the vitamin A that parts by weight are 0.2, the soybean oil that parts by weight are 143, the peak wax that parts by weight are 6; Described capsule shells comprises that parts by weight are 190 gelatin, the glycerol that parts by weight are 70, the purified water that parts by weight are 190, the burnt sugar coloring that parts by weight are 9.

embodiment 3:

the blue berry xanthophyll soft capsule that improves vision described in embodiment 1, described content comprises that parts by weight are 47 Pericarpium Citri tangerinae extract, the zinc gluconate that parts by weight are 23, the vitamin C that parts by weight are 16, the phylloxanthin powder that parts by weight are 14, the vitamin E that parts by weight are 4, the vitamin A that parts by weight are 1, the soybean oil that parts by weight are 146, the peak wax that parts by weight are 9; Described capsule shells comprises that parts by weight are 210 gelatin, the glycerol that parts by weight are 90, the purified water that parts by weight are 210, the burnt sugar coloring that parts by weight are 10.

embodiment 4:

the blue berry xanthophyll soft capsule that improves vision described in embodiment 1, described content comprises that parts by weight are 45 Pericarpium Citri tangerinae extract, the zinc gluconate that parts by weight are 21.5, the vitamin C that parts by weight are 15, the phylloxanthin powder that parts by weight are 12.5, the vitamin E that parts by weight are 3.3, the vitamin A that parts by weight are 0.6, the soybean oil that parts by weight are 144.5, the peak wax that parts by weight are 7.6; Described capsule shells comprises that parts by weight are 200 gelatin, the glycerol that parts by weight are 80, the purified water that parts by weight are 200, the burnt sugar coloring that parts by weight are 9.6.

embodiment 5:

as shown in Figure 1.

embodiment 6:

the blue berry xanthophyll soft capsule that improves vision described in embodiment 1, described Pericarpium Citri tangerinae extract is one of fruitlet fruit trees seeds, belongs to Ericaceae Vaccinium (Vaccinium) plant, originates in North America.Pericarpium Citri tangerinae is perennial undershrub fruit tree, and fruit is berry, blue or red, subcircular.Wherein blue fruit Pericarpium Citri tangerinae worldwide cultivated area is maximum, be commonly called as blue berry, the research of the U.S. shows, in Pericarpium Citri tangerinae fresh fruit containing rich in protein and VA, VC, VE, except containing conventional sugar, acid, cellulose and mineral element.The more important thing is special compound compositions such as being rich in antioxidant, bacterial growth inhibitor, ellagic acid, elladitannin, folic acid, procyanidin, flavonoid.The procyanidin of cranberry has good health-care effect to eyes, can alleviate eyestrain and improve night vision.To suffering from the retinopathy that diabetes and diabetes cause, there is good medical effect, also can prevention.Cranberry extracting solution can improve people's amblyopia, accelerates rhodopsin and lives again, and improves nyctalopia or dark adaptation disease, improves the recovery capability of eyestrain.Asthenopia and amblyopia etc. is had to auxiliary therapeutic action.

described Pericarpium Citri tangerinae extract is the procyanidin that extracts from Pericarpium Citri tangerinae, and procyanidin can improve vision, it is dizzy to alleviate; Can protect blood capillary, promote to look regeneration of erythrocytes, strengthen dark adaptive capacity; Can improve noctovision, alleviate visual fatigue, improve the adaptive capacity of low-light level.Europe be approximately used as eye health articles for use from nineteen sixty-five.Procyanidin function of relieving visual fatigue mechanism is mainly that procyanidin acts on retinal isomerase, increased rhodopsin generating rate, rhodopsin is the photoactive substance of retina optic cell, when rhodopsin is subject to light to amphiblestroid stimulation, can decompose in moment, and this chemical change is sent to brain, thereby generation " visible ".Rhodopsin can natural resynthesis when dark place, and procyanidin can promote rhodopsin resynthesis in the dark, improves the susceptibility of retina to light.The impact of anthocyanidin on near-sighted teenager asthenopia symptom and vision.Adopt double-blind method, to 326 near-sighted teenager, be divided at random treatment group and matched group.Treatment group gives oral anthocyanidin 200mg every day, matched group every day oral starch 200mg.After one month, observe the variation of eye symptom, vision and refraction of eye degree, data is carried out statistical analysis.Result shows: compare with matched group, anthocyanidin treatment group eye symptom improves obviously, is respectively blurred vision and improves 77.2%, eyeball swells 84.9%, ophthalmalgia 93.1%, photophobia 50.6%, eye is dry and astringent 87.3%, the tired sense of eyeball acid 81.9% (P<0.05); Low myopia and early stage myopia distant vision on average improve 2 ~ 3 row (logarithmic visual acuity chart) (P<0.05); But on the impact of eye refraction degree without significance (P>0.05).Reach a conclusion: anthocyanidin can effectively improve asthenopia symptom, improve the distant vision of early stage myopia and low myopia.

described Pericarpium Citri tangerinae extract compound vitamin A is to improving asthenopic effect.Method: select 120 of the fatigable experimenters of vision, be divided into test-meal group and comfort group, every group of 60 people, the oral Pericarpium Citri tangerinae extract compound vitamin of test-meal group A every day 2 times, each l grain, the oral color of matched group, the placebo that character is identical with Pericarpium Citri tangerinae extract compound vitamin A capsule, amount is with test-meal group, and duration of trial is 30d.Then carry out following inspection: normal observation, security inspection, eye symptom inspection, observation eye subjective symptoms, ophthalmology routine examination, visuognosis persistence test, examination of visual acuity.Result: after 30d, test-meal group asthenopia obviously alleviates, eyes duration of photopic vision on average improves 13.23% ± 10.34%, total effective rate 51.9%, and matched group duration of photopic vision on average improves 1.39% ± 4.74%, total effective rate 5.8%, learning by statistics processing difference has significance.Conclusion: research shows that Pericarpium Citri tangerinae extract compound vitamin A can effectively alleviate human body asthenopia.

described Pericarpium Citri tangerinae extract compound vitamin A is to improving asthenopic effect.Method: select 120 of the fatigable experimenters of vision, be divided into test-meal group and comfort group, every group of 60 people, the oral Pericarpium Citri tangerinae extract compound vitamin of test-meal group A every day 2 times, each l grain, the oral color of matched group, the placebo that character is identical with Pericarpium Citri tangerinae extract compound vitamin A capsule, amount is with test-meal group, and duration of trial is 30d.Then carry out following inspection: normal observation, security inspection, eye symptom inspection, observation eye subjective symptoms, ophthalmology routine examination, visuognosis persistence test, examination of visual acuity.Result: after 30d, test-meal group asthenopia obviously alleviates, eyes duration of photopic vision on average improves 13.23% ± 10.34%, total effective rate 51.9%, and matched group duration of photopic vision on average improves 1.39% ± 4.74%, total effective rate 5.8%, learning by statistics processing difference has significance.Conclusion: research shows that Pericarpium Citri tangerinae extract compound vitamin A can effectively alleviate human body asthenopia.The procyanidin (procyanidin, PC) of various dose causes the antagonism of retinal ganglial cells (retinal ganglion cells, RGCs) damage to microwave.Method: In vitro culture RGCs, cell is divided into 6 groups at random, be 4 experimental grouies of different PC dosage (0 μ g/L, 2 μ g/L, 20 μ g/L and 40 μ g/L) and vitamin C 40 μ g/L group and matched groups, giving respectively frequency is the microwave continuous irradiation 1h that 2450MHz, power density are 30mW/cm2, and matched group does not give irradiation.The change of morphocytology after light Microscopic observation predose, Trypan Blue detects cell survival rate, application flow cytometer and DNA end-labelling (TdT-mediated-dUTP nick end labeling, TUNEL) detection by quantitative early apoptosis of cells.Result shows: after microwave exposure, cell has clustering phenomena, and part cell process disappears.0h after irradiation, 20 μ g/L and 40 μ g/L PC groups are compared with the apoptosis rate of 0 μ g/L PC group decrease (P<0.05); 6h and 12h after irradiation, 3 PC dosage groups have reduction (P<0.05) compared with the apoptosis rate of 0 μ g/L PC group; 18h after irradiation, only has 40 μ g/L Pc groups significantly to reduce (P<0.01) compared with the apoptosis rate of 0 μ g/L PC group.Conclusion: microwave can be induced RGCs apoptosis, PC has certain antagonism to this damage.The procyanidin (procyanidin, PC) of various dose causes the antagonism of retinal ganglial cells (retinal ganglion cells, RGCs) damage to microwave.Method: In vitro culture RGCs, cell is divided into 6 groups at random, be 4 experimental grouies of different PC dosage (0 μ g/L, 2 μ g/L, 20 μ g/L and 40 μ g/L) and vitamin C 40 μ g/L group and matched groups, giving respectively frequency is the microwave continuous irradiation 1h that 2450MHz, power density are 30mW/cm2, and matched group does not give irradiation.The change of morphocytology after light Microscopic observation predose, Trypan Blue detects cell survival rate, application flow cytometer and DNA end-labelling (TdT-mediated-dUTP nick end labeling, TUNEL) detection by quantitative early apoptosis of cells.Result shows: after microwave exposure, cell has clustering phenomena, and part cell process disappears.0h after irradiation, 20 μ g/L and 40 μ g/L PC groups are compared with the apoptosis rate of 0 μ g/L PC group decrease (P<0.05); 6h and 12h after irradiation, 3 PC dosage groups have reduction (P<0.05) compared with the apoptosis rate of 0 μ g/L PC group; 18h after irradiation, only has 40 μ g/L Pc groups significantly to reduce (P<0.01) compared with the apoptosis rate of 0 μ g/L PC group.Conclusion: microwave can be induced RGCs apoptosis, PC has certain antagonism to this damage.The impact on xerophthalmia by dosage form of anthocyanidin (procyanidins, PC) eye.Method: by 40 examples (40 eyes) patients with dry eye, by complete randomly assigne, be divided into 4 groups, low (the 2 μ g/L) of different PC dosage, in (20 μ gL), high (40 μ g/L) 3 experimental grouies and negative control group (normal saline).Before medication and after medication the 3rd, 7 days, inquiry patient subjective symptoms, asked while checking Schimer's test (Schirmer test, ST), tear film rupture (tear film break-up time, BUT).Result shows: after medication, patients with dry eye subjective symptoms is improved; BUT is extended, have statistical significance (P<0.01); ST affected to there was no significant difference (P>0.05).3 kinds of dosage eye drops have significant difference (P<0.01) to BUT and ST compared with matched group.Reach a conclusion thus: PC eye drop has good therapeutic effect to xerophthalmia.The concentration higher (between 2 ~ 40 μ g/L) of PC eye drop, administration time longer (within 7 days), more obvious to patients with dry eye indices improvement effect.

embodiment 7:

the blue berry xanthophyll soft capsule that improves vision described in embodiment 1, zinc in described zinc gluconate is the trace element of needed by human, it is connected closely with child's growth promoter, immunologic function, vision and sexual development, and zinc supplement can promote the normal development of children's torso and intelligence; Can strengthen the sensitivity of optic nerve, zinc deficiency can affect vitamin A absorption in vivo.The relation of zinc and vision, is mainly by zinc enzyme (alcoholdehydrogenase), vitamin A (retinol) to be oxidized to optically active substance in rod cell to form retinal, is convenient to form visual pigment, in order to improving Infravision.In eye tissue, retina and choroidal complex are the highest containing zinc, and several zinc enzymes comprise that the level of carbonic anhydrase and retinol dehydrogenase is all very high, and the latter can be oxidized to retinal retinol, i.e. the form of vitamin A in rod cell.Night vision depends on to rod cell and provides appropriate retinal to form visual pigment---rhodopsin.The phenomenons such as zinc deficiency can make that people occurs that dark adaptation ability weakens, visual deterioration, myopia, hypermetropia and astigmatism.

4 ~ May in 2007, investigate 396 pupils' in Yinchuan City vision, analyzed the relation of poor sight and Zn in hair content.Method: choose 396, city, Yinchuan City pupil, check its standard logarithmic vision.60 students that choose the equal <1.0 of eyes naked vision are experimental group, and the student who extracts 60 naked vision >=1.0 of eyes is matched group, adopt atomic absorption spectrophotometry to measure the content of Zn in hair.Result shows: the pupil's poor sight person who investigates accounts for 27.53% of total number of TB suspects examin ed.The content of binocular vision poor students Zn in hair is compared with normal visual acuity student, and difference has statistical significance (the equal <005 of P value).Conclusion draws: school age population vision health relevant with zinc-emitting content.The zinc of usining is studied the lipid peroxidation injury effect of microwave to the porcine retinal pigment epithelial cells of In vitro culture as protection factor.Method: In vitro culture porcine retinal pigment epithelial cells, by microwave exposure intensity difference, be divided into matched group, 30,20,10 mW/cm2 groups and each irradiation dose zincification group, microwave therapy apparatus is in the worn-out indoor irradiation 1h of microwave screen, respectively at 24h observation of cell metamorphosis after irradiation, measure superoxide dismutase (SOD) and malonaldehyde (MDA) content.Result shows: after microwave exposure, cell has clustering phenomena, and part cell takes off wall, and cell MDA content obviously increases, SOD activity decreased; Zincification is respectively organized under light microscopic cellular morphology and is changed not obviously, and MDA content more not zincification group recovers to some extent, and SOD activity also increases; Irradiation after zincification, 3 groups of cell MDA are all than not zincification group reduction, SOD increased activity.Conclusion: microwave can cause retinal pigment epithelium lipid peroxidation injury, zinc can improve the oxidation resistance of cell, alleviates to a certain extent the peroxide injury of microwave to retinal pigment epithelium, and its action effect is relevant with concentration.

by observing variation and the protective effect of zinc ion to diabetic retinal tissue in rat of diabetic retinal tissue in rat reduced glutathion and lipid peroxidation product mda content.Method: set up the diabetes model of streptozotocin (Streptozotocin, STZ) induction, be divided at random Normal group, diabetic model group and zinc chloride intervention group, 24 every group, observe 72h.After processing, 24h, 48h, 72h get blood and tissue, application spectrophotometry pancreas and retina reduced glutathion (glutathione, GSH); The mensuration of retina malonaldehyde (Maleic Dialdehyde MDA) adopts TBA method; The detection radioimmunology of serum insulin levels.Result: each index content indifference of normal group; Model group blood glucose value, retina MDA increased content, 24h point insulin level raises, and 48h and 72h reduce, and pancreas and retina GSH content reduce; Intervention group measurement index in each between between the two in period.Reach a conclusion: zinc ion can improve diabetic retinal tissue in rat GSH content and reduce MDA level, strengthens its stress ability, likely become a kind of effective medicine of prevention and treatment of DR.

by observing the reaction of retina zinc transporter 3 (ZnT-3) mrna expression to low zinc diet, explore the adjusting whether low zinc can cause ZnT-3 transcriptional level.Method: 36 male SD rats (4 week age) are divided into three large groups at random and feed respectively 2 weeks (the 1st group), 4 weeks (the 2nd group), 6 weeks (the 3rd group), every group of rat was divided at random again two subgroups and gives respectively normal zinc content diet and low zinc diet, and the 3rd group low zinc diet rat recovered normal diet since the 4th week.By reverse-transcription polymerase chain reaction (RT-PCR) method, detect the mRNA relative expression quantity of ZnT-3 and carbonic anhydrase 2 (CA2), carbonic anhydrase 14 (CA14).Result shows: rat zinc is taken the photograph people after low 2 weeks, and the mRNA level of comparing retina CA2 and CA14 with blank group decreases, and ZnT-3 mrna expression amount increases to some extent; Zinc is taken in after low 4 weeks, and retina ZnT-3, CA2 and CA14 mrna expression amount all obviously decline: recover after normal zinc diet the expression rise of the mRNA of experimental group retina ZnT-3, CA2 and CA14 and matched group zero difference 2 weeks.Conclusion: the variation of diet zinc is by the adjusting that causes rat retina ZnT-3 on transcriptional level. zinc enzyme CA2 and CA14 mrna expression also change simultaneously.For stablizing of zinc content in maintenance retinal tissue, time and degree that ZnT-3mRNA expression reduces with diet zinc content change.Illustrate that zinc and retinal function are in close relations.

by observing the impact of zinc on diabetic retinal tissue in rat reduced glutathion and the variation of lipid peroxidation product mda content and retinal neuronal cell apoptosis and the expression of apoptosis-associated genes.Method: 24 Wistar rats, be divided at random 3 groups, after 7 weeks, put to death, detect amount and blood glucose and the serum insulin levels of reduced glutathion (glutathione, GSH), malonaldehyde (Maleic Dialdehyde MDA).TUNEL technology for detection apoptosis situation, Use immunohistochemistrySP SP is measured Bcl-2, Bax and Caspase-3 protein expression level.Result shows: compares with normal group, and model group blood glucose value, MDA increased content, insulin level and GSH content reduce, and each index for the treatment of group is between between the two; Model group rat retina neuronal apoptosis is counted showed increased, and Bcl-2 positive cell number reduces, and Bax and Caspase-3 protein expression level increase; Treatment group and model group comparison, TUNEL positive expression reduces, and Bcl-2 positive cell number increases, and Bax and Caspase-3 protein expression level decline.Conclusion: zinc not only can improve diabetic retinal tissue in rat GSH content and reduce MDA level, strengthens its stress ability; Zinc also has anti-apoptotic effect.

preventive and therapeutic effect by research metallic zinc to type 2 diabetes mellitus rat retina pathological changes (diabetic retinopathy, DR).38 Wistar rats of method are divided into 4 groups at random: the Normal group that normal feedstuff is fed, high fat+zinc group that zinc supplement high lipid food is fed, set up diabetic retinopathy model high fat forage feed high glucose and high fat group, set up high glucose and high fat+zinc group of diabetic retinopathy model high fat forage feed zinc supplement; After 4W, gently pluck eyeball, under transmission electron microscope, observe and respectively organize retinal ultrastructure respectively.Result shows: under Normal group transmission electron microscope, retinal capillary endotheliocyte is positioned at tube chamber inner face, and outside has continuous basement membrane and pericyte to hold, and endotheliocyte nuclear morphology is normal, and heterochromatin is evenly distributed, and organelle form is normal; In a small amount of heterochromatin of capillary endothelial cell core Zhou Kejian under high fat+zinc group transmission electron microscope, core, be dispersed in as seen the heterochromatin of distribution, thickness of pipe wall normal, has tube chamber inner process thing, mitochondrion normal, and basement membrane is without thickening; Retinal capillary basement membrane thickened under high glucose and high fat group transmission electron microscope, fracture disappearance, endotheliocytic swelling distortion, nuclear morphology is irregular, and heterochromatin keeps to the side to assemble, mitochondrion cavitation, spinal fracture, pericyte's mitochondrial swelling distortion, cavitation, tube wall thickens, tube chamber obvious stenosis; Under high glucose and high fat+zinc group transmission electron microscope, the heterochromatin of retinal capillary endotheliocyte Visible Core periphery is assembled, also visible heterochromatin in core, mitochondrion is normal, thickness of pipe wall normal, visible a small amount of thrust in tube chamber, accidental cavitation in pericyte's core and mitochondrion.Conclusion: author thinks that supplementing metallic zinc can have preventive and therapeutic effect to rats with type 2 diabetes retinopathy microangiopathies.

the impact of rat diabetes retinopathy (diabetic retinopathy, DR) vascular endothelial cell growth factor (VEGF) being expressed by research metallic zinc, confirms the preventive and therapeutic effect of zinc to diabetic renal papillary necrosis.Method is set up diabetic renal papillary necrosis model with Wistar rat, is divided at random 4 groups: Normal group, normal zinc supplement group, high sugared group, high sugar+zinc group.After 3M, gently pluck eyeball, in the up VEGF SABC of retinal slice.Result shows: immunohistochemical staining VEGF positive expression in Normal group, normal+zinc group and high sugar+zinc group retina paraffin section, the obvious positive expression of high sugar group retina paraffin section VEGF SABC, high sugar group retinal slice VEGF SABC IOD value is compared obviously and is increased (P<0.001) with normal group, normal+zinc group and high sugar+zinc group, and normal group retinal slice VEGF SABC IOD value is compared there was no significant difference (P>0.05) with normal+zinc group and high sugar+zinc group.Conclusion: author thinks that supplementary metallic zinc can reduce the expression of diabetic retinal tissue in rat pathological changes VEGF, has the effect of control DR.

embodiment 8:

the blue berry xanthophyll soft capsule that improves vision described in embodiment 1, described vitamin C is one of composition forming eyeball crystalline lens.In human eye, ascorbic content is than exceeding 30 times in blood.With age, Vitamin C content obviously declines, and crystalline lens malnutrition can cause crystalline lens degeneration in the course of time; Vitamin C can weaken the oxygen of light to lenticular infringement, has the effect that prevents that old cataract from forming.According to doctor diagnosed, the ultraviolet in light can form the injury of radical pair tissue, at eyes, the most often causes cataract and old maculopathy, and these two kinds of diseases finally all may cause blind.Prevention, can do from improving the extracted amount of vegetable, especially contains vitamin C and E and carotene, can significantly reduce these diseases.Vitamin C is the important nutritional labeling of eye lens.In addition, vitamin C is also antioxidant, can remove eye free radical, eliminates eye tired.

observe the impact of VitC on the lower In vitro culture retinal capillary pericytes propagation of high sugar, inquire into the method for preventing and treating of diabetic renal papillary necrosis (DR).Method: the fresh buphthalmos ball of take is material, separation is also cultivated pericyte; Adopt 3H-TdR to mix method and liquid scintillation technology, the impact of the VitC of research variable concentrations (50,100,500,1000,1500mg/L) on pericyte's propagation.Result demonstration: 500, the VitC of 1000mg/L is on physiological concentration glucose 15mmol/L) not impact of lower known born of the same parents' propagation.Heavy dose of VitC (500,1000,1500mg/L) adds in high sugar (20mmol/L) culture fluid and cultivates the 48h of pericyte, and the propagation of peripheral cells has obvious facilitation.Conclusion: heavy dose of VitC can promote the propagation of Gao Tangxia pericyte, prompting VitC can be used as the active drug of clinical prevention DR.

observe vitamin C and E to the amphiblestroid protective effect of photic damage.Method: make Mouse Retina generation photochemical damage with light damage method, then use medicine (vitamin C and E) protection, the photic damage situation of last om observation medication group and matched group.Result shows: light with medicine group rat retina during photochemical damage, show as vertebra, rod cell layer edema, and a little size degradation, outer nuclear layer infringement is not obvious; Photic damage group shows as vertebra rod cell layer edema, size degradation, cavity change, outer nuclear layer arrangement disorder, stratum nucleare attenuation.Conclusion: vitamin C and E have obvious rescue effect to Mouse Retina photoreceptor layer and outer nuclear layer.

the impact of research vitamin C on diabetic retinal tissue in rat capillary ultrastructure.Method: 60 of SD rats are divided into 5 groups, Normal group (OON), diabetes group in March (DM3), diabetes group in June (DM6), diabetes add Vc3 month group (DM3+Vc), and diabetes add Vc6 month group (DM6+Vc).Rat by 60mg/kg tail vein injection streptozotocin (STZ) to prepare rat diabetes model.Conventional raising, to after date anesthesia, perfusion fixation, makes retina ultrathin section, transmission electron microscope observing.Result shows: ultrathin section is observed along with diabetic duration development, retinal capillary endotheliocyte, pericyte's chromatic agglutination, karyorrhexis, mitochondrion generation cavity becomes, basement membrane thickened, blood capillary luminal stenosis is even inaccessible, and after application Vc, form change obviously alleviates.The form that conclusion: Vc can delay diabetic retinal tissue in rat blood capillary changes, and rat retina blood capillary is had to certain protective effect.

the impact of research vitamin C on diabetic retinal tissue in rat free radical system of defense.Method: 60 of SD rats are divided into 5 groups, blank group (OON), diabetes March (DM3) group, diabetes June (DM6) group, diabetes add Vc3 month group (DM3+, Vc), and diabetes add Vc6 month group (DM6+Vc).Rat is prepared diabetes rat model by 60rng/kg tail vein injection streptozotocin.By rat anesthesia, take out eyeball, peel off retina, after homogenate, survey superoxide dismutase (SOD), malonaldehyde (MDA), the proud statistical analysis of SPSS10.0 software for result.Result: along with the development of diabetic duration, diabetic retinal tissue in rat SOD level reduces gradually, MDA level raises gradually, and DM3, DM6 group has highly significant (P<0.01) with OON group comparing difference.Supplement after Vc, the horizontal rebound significantly of diabetic retinal tissue in rat SOD, MDA level decreases, and DM3+Vc, DM6+Vc group DM3, DM6 group comparing difference has highly significant (P<0.01).It is active that conclusion: Vc can improve retinal tissue SOD, strengthens its oxidation resistance, reduces MDA and produce, thereby diabetic retinal tissue in rat free radical system of defense is played to certain protective effect.

observe the impact that vitamin C (Vit C) is expressed B cell lymphoma (Bcl-2) in rabbit retinal pigment epithelium after illumination (retinal pigment epithelium, RPE) cell.Method: use the RPE cell of enzyme digestion Isolation and culture pigment rabbit, cause its apoptosis with radiation of visible light.Observe vitamin C (Vit C) impact on rabbit RPE cell Bcl-2 expression at different administration times of various dose.Result shows: pigment rabbit RPE cell after radiation of visible light in cell Bcl-2 all have expression in various degree, it expresses quantity and Vit C dosage is proportionate.Conclusion: Vit C can increase the expression of rabbit RPE cell Bc1-2 after illumination, it may play the effect that suppresses photic damage, reduces apoptosis.

by Difrarel associating vitamin C treatment of diabetic retinopathy, become.Specific as follows:

physical data: choose and be diagnosed as DR patient's 93 examples, male 43 examples, female's 50 examples, 51～73 years old age.Be divided at random matched group 48 examples, male 22 examples, female's 26 examples, 52～73 years old age, mean aves 59.30 scholar 12.14 years old.Treatment group 45 examples, male 21 examples, female's 24 examples, 51～72 years old age, average 59.20 scholar 12.12 years old.Two groups of Genders, age, the course of disease, symptom and sign, optical fundus typing by stages and every laboratory checking index comparison, are learned and are processed without significant difference by statistics.

method: matched group: persantin (25mg/ sheet) 200mg/d, aspirin (25mg/ sheet) 300mg/d, kallidinogenase (YIKAI 60U/ sheet) 240U/d therapeutic alliance.Treatment group: Difrarel (0.4g/ sheet, every containing bilberry extract 100mg, beta-carotene 5mg) 1.2g/d, vitamin C 600mg/d.15d was 1 course for the treatment of, medication 60～90d (4～6 course for the treatment of).

the judgement of observation item and curative effect: take patient's vision, microaneurysm, retinal hemorrhage, ooze out, the change situation such as edema is to observe foundation.1. it is effective: more than patient's vision improves 1～2 row; Examination of ocular fundus, microaneurysm is obviously controlled, retinal hemorrhage, ooze out, edema obviously reduces or absorbs; 2. invalid: vision is unchanged or decline, microaneurysm, retinal hemorrhage, ooze out, edema is unchanged or increase the weight of.

statistical procedures: adopt SPSS11.0 statistical package to carry out statistical procedures, data adopts chi-square test of four-fold table, data are with (analyze between group with chi-square criterion and t check, P<0.05 is that difference has statistical significance.

result: treatment group 45 examples, effective 37 examples of eyesight improving (82.22%), invalid 8 examples (17.78%), treatment group is better than matched group to the curative effect of eyesight improving situation, and two groups of comparing differences have statistical significance (P<0.05). microaneurysm is obviously controlled (effectively) 35 examples (77.78%), invalid 10 examples (22.22%), the curative effect that treatment group is controlled situation to microaneurysm is better than matched group, and two groups of comparing differences have statistical significance (P<0.05). retinal hemorrhage, ooze out, edema is clearly better or absorb (effectively) 36 examples (80.0%), no effect 9 (20.00%), treatment group to retinal hemorrhage, ooze out, curative effect that edema pathological changes is improved situation is better than matched group, two groups of comparing differences have statistical significance (P<0.05).

conclusion: this group observed result shows that Difrarel associating vitamin C treatment of diabetic retinopathy becomes, few side effects, curative effect are better.

embodiment 9:

the blue berry xanthophyll soft capsule that improves vision described in embodiment 1, described phylloxanthin powder has another name called " plant progesterone ", at occurring in nature and zeaxanthin, jointly exists.It is the key component that forms the plant pigment such as Semen Maydis, vegetable, fruit, flowers; be contained in the chloroplast of leaf; the luminous energy of absorption can be passed to chlorophyll a, infer photooxidation, photo damage are had to protective effect, be also the main pigment that forms human eye retina's macular region.Phylloxanthin be present in human body eye institute in a organized way in, comprise iris tissue, crystalline lens, retina, optic nerve and eye fat, wherein the highest with macula retinae district concentration.Macular area has been concentrated a large amount of cone cells, is the sharpest region of vision, keeps macula lutea health most important for keeping normal vision function.And human body can not synthesize phylloxanthin, can only supplement by taking in.Many Epidemiological study results demonstrations, there is positive correlation in the intake of phylloxanthin and macula retinae pigment density, and a certain amount of phylloxanthin of daily iron supplement can make vision make moderate progress.Current studies show that, the mechanism of phylloxanthin vision protection mainly contains optical barrier effect and antioxidation 2 points.Phylloxanthin can absorb a large amount of blue lights, and the wavelength of blue visible light and ultraviolet light approach, and are a kind of light that can reach potential hazard maximum in amphiblestroid visible ray.Before light arrives cell responsive on retina, first pass through the highly the densest district of phylloxanthin, if the lutein content of macula lutea is abundant, just this injury can be reduced to minimum.In addition, phylloxanthin is a kind of stronger antioxidant, has the effect of removing free radical and free radical, can suppress the oxidative damage of the tissues such as retina that many reasons causes, crystalline lens, macula lutea, to safeguard twenty-twenty vision.Based on above mechanism of action, phylloxanthin is applied in asthenopic control.There are some researches show, the health food that the phylloxanthin of take is main component is for asthenopia 202 examples, and result shows to have asthenopia releasing function.

observe the experimental study of phylloxanthin to the effect of rat retina blue light injury protection.Method: rat is divided into Normal group, model control group, solvent control group, the basic, normal, high dosage group of phylloxanthin at random.The concentration of the basic, normal, high dosage group of phylloxanthin is respectively 0.5,1.0 and 2.0mg/ml, and normal saline and Tween-80 are mixed with solvent with the ratio of l:9.In phylloxanthin group and solvent control group rat vitreous body, inject respectively various dose phylloxanthin and solvent, injection volume is 5 μ l, and dark adaptation 24h, sets up retinal photic injury in rats animal model with blue photo-damage device, light open-assembly time is 2h, and after light exposes, raise in darkroom.After 72h, win eyeball and prepare wall of eyeball paraffin section, micro-Microscopic observation retinal morphology changes, and measures outer nuclear layer thickness.And count outer nuclear layer apoptotic cell.Result shows: each dosage phylloxanthin group rat and model control group ratio, and retinal structure is well arranged, and cell arrangement is neat; Outer nuclear layer thickness (40 * 10 times), Normal group is (21.25 ± 1.04) mm, model control group and solvent control group are respectively (3.25 ± 1.48) mm and (3.25 ± 0.89) mm, the basic, normal, high dosage group of phylloxanthin is respectively (15.00 ± 5.58) mm, (11.75 ± 4.20) mm and (14.75 ± 3.96) mm, is all significantly higher than model control group (P<0.01).But this experiment adopts Tunel kit detection cell apoptosis, between each group, has no marked difference.Conclusion: under this experiment condition.Phylloxanthin has significant protective effect to retinal photic injury in rats.

adopt the research of double blind random blank, at random 37 22～30 years old long-term fluorescent screen light resurrectionists are divided into low dose group, high dose group and placebo group, give respectively 6 every day, 12mg phylloxanthin and placebo, intervene continuously 12 weeks.Before intervention and intervene after 12 weeks, measure serum phylloxanthin concentration, and the visual function index such as while detecting breakup time of tear film, critical flicker fusion frequency, duration of photopic vision, vision response.Result shows: after phylloxanthin is intervened, high dose group and low dose group serum phylloxanthin (μ mol/L) level rise to 0.733 ± 0.354 and 0.6074 ± 0.176 by 0.328 ± 0.120 and 0.356 ± 0.117 respectively.During the film rupture of high dose group tear, ask and intervene front significant prolongation, duration of photopic vision significantly improves, and vision simple reaction time is intervened front obvious shortening; After intervening when critical flicker fusion frequency, visual discrimination reaction and choice reaction time without significant change.Final definite, after phylloxanthin is intervened, long-term fluorescent screen light resurrectionist breakup time of tear film, duration of photopic vision and simple reaction time all improve significantly.Phylloxanthin exempts from photoinduced free radical damage as oxidant protective plant.Blue light be in all visible rays energy the highest and can mediate photooxidation that active oxygen causes stress, phylloxanthin can absorb a large amount of blue lights.Supplementary phylloxanthin can make long-term fluorescent screen light resurrectionist duration of photopic vision improve, and has the effect of the long-term fluorescent screen of protection light resurrectionist visual function.

with eye, asthenopic adult, be divided into 2 groups by 402 examples are long-term at random, the equal tool comparabilities such as two groups of ages, sex, the course of disease, distant vision, duration of photopic vision.Observation group takes containing phylloxanthin health food, and matched group is taken placebo, 4 weeks courses for the treatment of.Period in a medicine does not change former dietary habit, normal diet.Experimenter's blood before and after test-meal, urine, just routine and blood biochemistry index are without significant change, and observation group's eyes duration of photopic vision on average improves 13.49%, and total effective rate is 63.37%, and more all there were significant differences with matched group.Comparative control group, all obviously improvement effects of symptom such as the blurred vision of observation group, dry and astringent, the ophthalmic bloated of eye, ophthalmalgia, photophobia, illustrates and contain the function that phylloxanthin health food has remitting vision fatigue.

on the basis that the applicable cases of approved registration alleviating asthenopia health food phylloxanthin is analyzed, further carry out human experiment function test, to inquire into single edible doses phylloxanthin to asthenopic mitigation, the method adopting is that random double-blind method is about to 120 fatigable experimenters of vision and is divided into test-meal group and placebo group, every group of each 60 people, test-meal group is taken phylloxanthin sheet, matched group is taken placebo, be every day 1 time, each 1 (10mg), Time of Administration is 30d.Carry out following inspection: sign observation, security inspection, ophthalmology routine examination, the inspection of eye subjective symptoms, visuognosis persistence test, examination of visual acuity.Found that the sense of test-meal group asthenopia obviously alleviates, duration of photopic vision on average improves 21.90, total effective rate 62.00, and symptom integral reduces 2.66 ± 1.44.Before and after test-meal, between test-meal group self comparison and group, learn more by statistics to process and have significant difference; With matched group comparison, the symptoms such as test-meal group experimenter blurred vision, dry and astringent, the eye of eye, ophthalmalgia, photophobia are improved.Illustrate and take the photograph the function that people 10mg phylloxanthin has alleviating asthenopia every day.This test is the exploratory study to the reasonable intake of phylloxanthin.Proved that every day, edible l0mg phylloxanthin was safe and effective to vision fatiguability person.

according to the check of Ministry of Public Health < < health food and value disciplines > > (version in 2003), phylloxanthin chewable tablet has been carried out scheduling to last to the human experiment clinical observation of 30d.Result shows: test group right and left eyes distant vision is all than making moderate progress before test-meal, and difference has statistical significance (P<0.05); Comparing difference not statistically significant (P>0.05) before right and left eyes distant vision and test-meal after matched group test-meal.After test-meal, test group duration of photopic vision 55.02 ± 8.70 before by test-meal 65.84 ± 11.60 after bringing up to and taking, has on average improved 21.90%, and front and back comparing difference has statistical significance (P<0.001); 56.71 ± 6.95 56.1l ± 9.31 brought up to take after of matched group duration of photopic vision before by test-meal, front and back comparing difference not statistically significant (P>0.05), after test-meal, test group duration of photopic vision is compared difference with matched group statistical significance (P<0.05).The eye symptom integration of test group is reduced to 4.48 ± 2.24 after taking by taking 7.14 ± 2.39 before tested material, and front and back comparing difference has statistical significance (P<0.001); After test-meal, the eye symptom integration of test group and matched group (5.84 ± 2.40) have statistical significance (P<0.05) compared with difference.Test-meal group total effective rate (62.00%) higher than matched group (14.00%) (P<0.05).Before and after test-meal group and matched group test, have no untoward reaction, every safety indexes is showed no extremely.Conclusion: evidence phylloxanthin human body is there is to the health care of alleviating asthenopia, and health is had no adverse effects.

embodiment 10:

the blue berry xanthophyll soft capsule that improves vision described in embodiment 1, described vitamin E is ophthalmology, department of eye conventional medicine clinically, the lipid peroxidation that can obviously suppress the pathological corneas of blister sore toxicity keratitis, shortens pathological changes healing time, reduces complication and sequela.Vitamin E still can suppress the formation of cicatrix, promotes corneal wound healing.Glycosaminoglycan (GAG) exists with the form of protein polysaccharide. and be one of important extracellular matrix.The extracellular matrix of trabecular tissue can regulate the transhipment of the inside and outside material of little fine strain of millet histiocyte and aqueous humor to discharge, it is relevant with the metabolism of GAG in little fine strain of millet tissue that the intraocular pressure of primary open angle glaucoma raises. and recent result of study shows, vitamin E can increase the total amount of the little fine strain of millet histiocyte of the human eye glycosaminoglycan of In vitro culture, increase hyaluronic constituent ratio simultaneously, on pathogenesis, have in the probability normal eyes retina of the open angle glaucoma of preventing and treating and contain abundant antioxidase as superoxide dismutase, glutathion peroxidase etc. and low molecular antioxidant etc., this plays an important role to the normal visual function of retina.Vitamin E is distributed widely in retina, the highest with the content of acromere and pigment epithelium cell.Zoopery shows, vitamin E is pressed the dosage single medication of 100mg/kg body weight, vein, intramuscular injection and mouthful its retina intensive amount of medicament for the eyes group all increase 3 ~ 5 times compared with matched group. but intravenously administrable group peaking is 2 ~ 4 hours, maintaining 168 hours. intramuscular injection and oral being respectively 24 hours and 72 hours peakings, maintain 192 hours.Human eye retina's content of vitamin E is higher than choroid and vitreous body, and the content of the high person's retina of serum VE level VE is also high.Have experiment to confirm that amphiblestroid lysosome membrane and acromere membranous disc have good Stabilization, vitimin supplement E can suppress in acromere the activity of S-acetyl-coenzyme-A and phospholipase A can be slowed down the membranous disc causing because of oxidative damage and come off.Vitamin E is as the vitamin of liposoluble, and it not only participates in biomembranous composition, or the interior important free radical scavenger of blood, tissue and cell and the blocker of lipid peroxidation chain reaction.Due to the particularity of eyeball anatomical structure and biochemical composition, be easily subject to the infringement of the many factors such as light, active oxygen, cause many ocular disease, make the function of eye impaired.Therefore, from meals, take the photograph the enough vitamin Es of people, necessary to keeping the normal physiological function foot of eye, and under some pathology and special environmental condition, when body lipid levels of peroxide increases, still need and increase the picked-up of vitamin E to prevent or to alleviate the damage of ocular tissue.

retinitis pigmentosa can cause intraretinal staff cell and cone cell to degenerate, and symptom comprises: nyctalopia, peripheral vision are impaired, finally can cause tubular visual field, lose chromatic discrimination power etc.The extent of damage based on different cells, patient there will be above any so that whole symptoms.Symptom starts appearance at patient's childhood or adolescence conventionally, and state of an illness development is until spend the remaining years till death.As for development speed and degree visually impaired, vary with each individual.Contribute to solve the query of retinitis pigmentosa.The staff cell of known patients with retinitis pigmentosa is dead because of sudden change, does not but know why cone cell meeting is dead.Studies confirm that, after staff cell death, the amount of oxygen in retina rises, and this is the reason of killing gradually cone cell.This oxygen injury is called again oxidative damage, so utilize antioxidant to reduce injury.

the content of malonaldehyde in retina after employing sulfo-barbital fluorescence spectrometry lagophthalmos acute high intraocular pressure, to reflect the amphiblestroid damage of radical pair; Apply free radical scavenger vitamin E simultaneously and observe the effect of antioxidant in removing free radical.Result: experimental eye the 1st day mda content after experiment raises, within the 3rd day, reach peak, after this start to decline, but in the time of the 60th day, do not recover yet normal level, in whole experimentation, experimental eye is all higher than contrast eye, and difference has utmost point significance (P<0.01).Not obvious with vitamin E treatment group l days therapeutic effect after experimental therapy, within the 3rd day, start mda content lower than experimental group, through F, check the two difference to have utmost point significance P<0.01), therapeutic effect is obvious.On a declining curve in experiment the 7th, l5,30 days malonaldehyde levels, have significance P<0.05 with experimental group comparing difference).No significant difference between the 60th day treatment group and experimental group.This experimental result confirms, it is that the lipid peroxidation product mda content of mediation increases that high intraocular pressure can make to take in retinal tissue free radical, in retina, the generation of free radical explanation free radical and metabolite are cause and increase the weight of high intraocular pressure to one of Pathophysiology factor of retina injury, and antioxidant vitamin E's should.With suppressing lipid peroxidation.Alleviate the damage of radical pair tissue.Play the amphiblestroid effect of protection.

the protective action of research operating microscope light source on the impact of Guinea Pig Retina and vitamin E (Vitamim E, VE).Method: irradiate guinea pig eye 15,30,60 and 90 minutes, and in illumination before 60 minutes hour and after illumination 1 and 6 hour with VE.Result shows: 15 minutes existing retinal ultrastructures of group of illumination obviously change and superoxide dismutase (Superoxid dismutase, SOD), glutathion peroxidase (Gluthione peroxidase, GSH-Px) obviously reduce, retinal ultrastructure degree of injury and SOD, GSH-P reduce, malonaldehyde (Malondialdehyde, MDA) raises and extends and be parallel relation with light application time.Cone cell injury journey is compared with rod cell weight.First 2 hours of illumination with VE to the protective effect of visual cell and Bruch film compared with illumination after 1 and 6 hour effective with VE, but VE to the damage of retinal pigment epithelium (Retinal pigmental epithelium.RPE) without obvious protective effect.Conclusion: operating microscope light source can cause retina photodamage, VE is effective protective agent.

the protective action of microwave on the impact of the porcine retina ganglionic cell form of In vitro culture and vitamin E (VE), damages the research put in order certain test basis is provided for microwave causes retina.By In vitro culture porcine retina ganglionic cell method, its morphosis of light microscopic and electron microscopic observation, adds variable concentrations VE, indoor with 30mW/cm2 power density irradiation lh in micro-wave screening by microwave therapy apparatus, after irradiation immediately in its metamorphosis of light Microscopic observation, its ultrastructure of electron microscopic observation.Relatively after predose and add the variation of retinal ganglial cells after VE (10,20 and 40g/L).Result shows: after microwave exposure, cell has clustering phenomena, and part cell axon disappears.Electronic Speculum object line plastochondria and endoplasmic reticulum swelling, VE respectively organize under light microscopic cellular morphology change not obvious, Electronic Speculum display line plastochondria mild swelling, ridge is complete.Result shows that microwave can cause the damage of retinal ganglial cells form, and VE can alleviate the damage of microwave to retinal ganglial cells to a certain extent.

the protective effect of vitamin E to the damage of retinal pigment epithelium DNA blue light.Method: retinal pigment epithelium is divided into 3 groups: normal group (not irradiation), experimental group (blue light illumination 40min), vitamin E group (blue light illumination gives vitamin E simultaneously).Adopt the method for rocket electrophoresis to analyze each group retinal pigment epithelium DNA damage.Result shows: each is organized rocket electrophoresis afterbody DNA content and is respectively 18.44%, 54.42% and 22.16%.Each organizes difference significant (P<0.01).Conclusion: blue light can produce damage to the DNA of retinal pigment epithelium, and vitamin E can play certain protective effect to damage.

the antiproliferative effect of VitE to the experimental proliferative vitreoretinopathy of lagophthalmos.Method: 15 healthy rabbits circulation of qi promoting body compressing vitreous body arts, after 3d, divide 3 groups, every group of 10 circulation of qi promoting liquid exchange.Matched group gives 0.5mL balanced salt solution; 50gVitE group gives the balanced salt solution of 0.5mL containing 50 μ g VitE; 100 μ gVitE experimental grouies give the balance liquid solution of 0.5mL containing 100 μ g VitE.Meanwhile, in 3 groups of vitreous body, also inject 0.1mL and contain 2.5 * 105 fibroblast suspension.With inspectoscope, observe optical fundus 4wk, a situation arises to record traction detachment of retina.After 28d, extract eyeball, make pathological section, light microscopy checking.Result shows: during 14d, matched group, 50 μ g VitE and 100 μ g VitE group mean P VR occurrence degree are 3.5,2.4,2.At the front 2wk observing, administration group has postponed the generation of PVR, and administration group has been compared difference (P<0.05) with matched group.This experimental result shows, the VitE saline solution (spirituosity 7.5mL/L) of intravitreal injection 50 μ g and 100 μ g can delay the generation of experimental PVR detachment of retina.

whether whether experimental proliferative vitreoretinopathy (PVR) forming process produce antioxidation to the lipid peroxidation occurring in PVR forming process with lipid peroxidation close ties and the intravitreal injection vitamin E of free radical initiation.Method: 18 healthy rabbits circulation of qi promoting body compressing vitreous body arts.Minute three groups of (every group of 12 lagophthalmos) circulation of qi promoting liquid exchanges after 3d.Model control group gives 0.5mL balanced salt solution; 50 μ g vitamin E groups give the balance liquid solution of 0.5mI containing 50 μ g vitamin Es; 100 μ g vitamin E groups give the balance liquid solution of 0.5mI containing 100 μ g vitamin Es.Meanwhile, in three groups of vitreous body, also inject 0.1mL and contain 2.5 * 105 fibroblast suspension.After gas-liquid exchange, 15d and 30d respectively get at random respectively 6 lagophthalmos from three groups. extract 0.6mL vitreous body, and separately get 6 normal lagophthalmos as Normal group, also extract 0.6mL vitreous body, then measure the content of lipid peroxide (LPO) and superoxide dismutase (SOD).Result shows: model control group is set up 15d and 30d at PVR model, LPO and SOD compared with normal matched group obviously raise (P<0.01) in vitreous body.In vitreous body, inject after vitamin E, the LPO of two administration groups obviously reduces (P<0.01) compared with model control group.SOD also reduces (P<0.05).This experimental result shows: in PVR forming process, exist significant lipid peroxidation.In vitreous body, inject the lipid peroxidation that vitamin E can suppress PVR forming process, bring into play powerful antioxidation.

cell model is also observed the impact that under anoxia condition, vitamin E is grown on cultivating retinal pigment epithelium.Method: test in 2006-10/2007-03 and complete in Nanjing Medical University's Physiological Experiment chamber.1. experimental implementation: the cell of the trophophase of taking the logarithm, take normal condition as the contrast (air that cellar culture and volume fraction are 0.95, volume fraction is 0.05 CO2), set up anoxia model (the anoxia cultivation of Human RPE Cells in Vitro, volume fraction is the CO2 mist that 0.95 N2 and volume fraction are 0.05, oxygen analyser oxygen determination concentration <1%, 24h), the vitamin E concentration that acts on cell is respectively 0.1,0.2,0.3mol/L.2. experimental evaluation: tetramethyl azo azoles salt method detects 24h cell viability (with absorbance A value representation); Laser confocal microscope detects reactive oxygen species level; Xanthine oxidase is measured superoxide dismutase activity in cell.Result: tetramethyl azo azoles salt detects Growth of Cells vigor: anoxia group cell viability is lower than matched group (P<0.01), anoxia+vitamin E group cell viability increases, compare significant difference (P<0.05) with anoxia group.Along with the raising of vitamin E concentration, vigor raises gradually.(reactive oxygen species level: with matched group comparison, the generation showed increased of anoxia group reactive oxygen species; With the comparison of anoxia group, anoxia+vitamin E group suppresses the generation of active oxygen in retinal pigment epithelium.3. superoxide dismutase activity detects: with matched group comparison, the vigor of anoxia group superoxide dismutase reduces; With the comparison of anoxia group, anoxia+vitamin E group superoxide dismutase activity raises, and difference all has significant (P<0.05).Conclusion: anoxia causes the damage of retinal pigment epithelium oxidative stress, and vitamin E has suppressed the damaging action of retinal pigment epithelium under anaerobic condition.

embodiment 11:

the blue berry xanthophyll soft capsule that improves vision described in embodiment 1, described vitamin A is the nutrient of needed by human, is also the main component of visual pigment, it has important effect to maintaining normal vision.When vitamin A deficiency or deficiency, can cause the reduction of dark adaptation ability, nyctalopia, cornea keratinization and visual disorder.Modern ophthalmology medical science thinks, when human body is deficient in vitamin A, can affect the synthetic of rhodopsin in eyeball, in eye muscle body, when rhodopsin approach exhaustion or wretched insufficiency, visual deterioration, blurred vision, nyctalopia phenomenon will occur.Because of the physiological action of vitamin A deficiency relevant with the scotopic vision of following, it can synthesize a kind of material that is rhodopsin in eyes, rhodopsin is to keeping normal good visual accommodation to play an important role, and relevant with the structural integrity and the enhancing development that maintain epithelial tissue.Pericarpium Citri tangerinae extract compound vitamin A, to asthenopic improvement effect, finds that teenager asthenope takes Pericarpium Citri tangerinae extract compound vitamin A capsule and can make asthenopia sense obviously alleviate, and eyes duration of photopic vision on average improves 13.23%, total effective rate 51.9%.The impact of vitamin A on coal mine underground operators night vision function, finds after the crowd's oral administration fish liver oil capsule treatment of down-hole, and dark adaptation time, low-light near vision and high light stimulate rear dark adaptation recovery time, retina light sensation absolute value all to have clear improvement fast.The relation of vitamin A, vitamin B1, vitamin B3, vitamin C, zinc, selenium, taurine etc. and night vision, points out that vitamin A plays an important role in especially dark adaptation function aspects of visual performance.

vitamin A and human body eye protection effect relation are very close, if vitamin A is undersupply or body malabsorption in meals, while causing vitamin A deficiency. be mainly manifested on people's eyes, occur that eyes are dry and astringent, night, blurring of vision etc., was commonly called as " flower order eye ".Ancient medical book nationality is " nyctalopia ", namely said on modern medicine " nyctalopia ".Vitamin A is the composition that forms photoactive substance in optic cell, and the generation of vision, is that it can become the signal that nervous system can be processed the stimulation of receiving from external environment owing to there being a kind of optics sensor on retina.The initial process of vision is in photoreceptor, to carry out opto-electronic conversion.Retina contains two kinds of photoreceptors, is a kind ofly short and thick taper shape cone cell, experiences high light and distinguishes that color mainly plays a role by day; Another kind is elongated rod bar spermatid, experiences decreased light or half-light, is mainly responsible for night vision.In people's eyes, above-mentioned two kinds of optic cells have, so day and night can see clearly object.In staff cell, have a kind of material of experiencing the low light level to be called rhodopsin, it is combined into by protein and vitamin A.If vitamin A undersupply, will have influence on the aggregate velocity of rhodopsin, although make human body can see thing clearly under the environment of high light, just differentiate unclear under decreased light or need the long period just can see clearly. Here it is is deficient in vitamin A and the nyctalopia that causes.Rhodopsin is present in membranous structure, combines with phospholipid.In the retina of human eye, existing retinal isomerase can its regeneration of catalysis. but organize the opsin of rhodopsin and retinal along with the elimination of being eaten of the outer section of a staff cell distal basin, carry out metabolism decomposition constantly.Therefore, constantly supplementing protein and vitamin A is the key factor that maintains rhodopsin regeneration, is also the important measures of safeguarding vision.The requirement of normal adult vitamin A every day is about 3,500 ius (every 1 iu is equivalent to the Hu Luosu of 0.3 microgram retinol or 0.6 microgram); Child is about 2,000～2,500 ius; Baby is about 1,500～2,000 iu; Women breast-feeding their children are about 4,500 ius; Anemia of pregnant woman is about 4,000 ius [35].

variation and the impact on neonate visual acuity of vitamin A (VitA) level in female blood, umbilical blood during intrauterine growth retardation (IUGR).Method: adopt VitA content in micro-fluorescence spectrometry IUGR group and each 30 routine maternal blood of Normal group, Cord Blood Serum.And adopt flash visual evoked potential (F-VEP) to detect neonate visual function development condition to all neonates.Result shows: IUGR organizes in female blood, umbilical blood VitA content all lower than Normal group (P<0.01), and VitA content dependency between the two in female blood, umbilical blood, r=0.8099, P<0.0l; VitA content and neonatal weight be proportionate (P<0.01) in female blood, umbilical blood.F-VEP detects IUOR group P100 compared with normal incubation period matched group and extends (P<0.05), and amplitude is lower than matched group (P<0.01); P100 incubation period and neonatal weight are negative correlation, and amplitude and neonatal weight are proportionate.Conclusion prompting: small for gestational age infant during IUGR (SGA) visual development is affected, this impact occurs to decline relevant with VitA content in female blood, umbilical blood.

the relation of coal mine operation workman nutritional status of vitamin A and night vision function and the improvement effect of supplementary vitamin A to coal mine underground operators night vision function in right amount.Method: select 101 of coal mine operation persons, 20 ~ 45 years old age, carry out 5d dietary survey and Deficiency disease inspection and dark adaptation aptitude tests, then everyone every day oral cod-liver oil 1 nine, after continuous 3 weeks, survey again dark adaptation.Observe therapeutic effect and treat the comparison of front and back.Meanwhile, select 98 of non-underground operators to compare.Result shows: the intake of two crowd's protein, heat energy, calcium, ferrum all accounts for the more than 90% of nutrient intake (RNI) of recommendation, but the poor distance of the intake of vitamin A, B2 and C and RNI is larger, only accounts for 50%～70% of RNI.Result of clinical detection shows, in underground work crowd, the symptom incidence rate such as dark adaptation time lengthening and conjunctival xerosis accounts for 28.7%, apparently higher than 3.06% in non-occupational population.The demonstration of dark adaptation check result, underground work crowd's dark adaptation ability is starkly lower than non-operator. and underground work is longer the length of service, and dark adaptation ability is poorer, shows the positive correlation trend with the underground work time limit.After down-hole crowd's oral administration cod-liver oil nine treatment, dark adaptation time, low-light near vision and high light stimulate dark adaptation afterwards recovery time, retina light sensation absolute value all to have clear improvement fast.This experimental result shows: supplement in right amount vitamin A coal mine operation workman night vision function is improved to effect.

with doing the test of alleviating asthenopia containing certain health food of vitamin A, vitamin C, vitamin E, phylloxanthin, zinc gluconate.Method: according to the check of Ministry of Public Health < < health food and assessment technique standard > > (version in 2003), this health food has been carried out scheduling to last to the effect assessment of the human feeding trial of 35d.Result shows: test group right and left eyes distant vision is all than making moderate progress before test-meal, and difference has statistical significance (P<0.05); Comparing difference not statistically significant (P>0.05) before right and left eyes distant vision and test-meal after matched group test-meal.After test-meal, test group duration of photopic vision has improved 0.12 ± 0.13, and after test-meal, test group duration of photopic vision and matched group compare difference statistical significance (P<0.01); The eye symptom integration of test group is reduced to 2.25 ± 1.86 after taking by taking 3.92 ± 1.94 before tested material, and front and back comparing difference has statistical significance (P<0.01); After test-meal, test group eye symptom integration and matched group (3.35 ± 1.85) comparing difference has statistical significance (P<0.01).Test group total effective rate (49.02%) higher than (3.92%) of matched group (P<0.05).Conclusion: test confirms that this health food has the effect of asthenopia releasing function to human body.

embodiment 12:

the blue berry xanthophyll soft capsule that improves vision described in above-described embodiment, the prepared main supplementary material that improves the blue berry xanthophyll soft capsule of vision of above-described embodiment is Pericarpium Citri tangerinae extract, zinc gluconate, vitamin C, phylloxanthin powder, vitamin E, vitamin A, soybean oil, Cera Flava, gelatin, glycerol, purified water, burnt sugar coloring, the health care of tool alleviating asthenopia.Phylloxanthin, vitamin A, zinc have the efficacy effect of definite alleviating asthenopia aspect; vitamin E, vitamin C have auxiliary protection function; health care is that the suitable crowd of the health food of " alleviating asthenopia " is " vision fatiguability person ", and therefore suitable crowd of the present invention is " vision fatiguability person ".

embodiment 13:

the blue berry xanthophyll soft capsule that improves vision described in above-described embodiment, the prepared capsule composition that improves the blue berry xanthophyll soft capsule of vision of above-described embodiment is gelatin, glycerol, pure water, the capsule shells that burnt sugar coloring is synthetic, wherein gelatin is a kind of using by the animal bone boiling, the peptide molecule polymeric material that skin and muscle tendon are made, it is the hydrolyzate of collagenic protein, contain a large amount of aminoacid, there is higher nutritive value, burnt sugar coloring with food stage saccharide as glucose, fructose, sucrose, Nulomoline, maltose syrup, corn syrup, molasses, glucidtemnss etc. are raw material, heating under more than 121 ℃ high temperature (or pressurization) makes it coking, and further process and make, human body is safe from harm.

embodiment 14:

the blue berry xanthophyll soft capsule that improves vision described in above-described embodiment, the prepared blue berry xanthophyll soft capsule that improves vision of above-described embodiment is according to the check of Ministry of Public Health < < health food and assessment technique standard > > (version in 2003), this health food has been carried out scheduling to last to the effect assessment of the human feeding trial of 35d, result shows: test group right and left eyes distant vision is all than making moderate progress before test-meal, and difference has statistical significance (P<0.05); Comparing difference not statistically significant (P>0.05) before right and left eyes distant vision and test-meal after matched group test-meal.After test-meal, test group duration of photopic vision has improved 0.12 ± 0.13, and after test-meal, test group duration of photopic vision and matched group compare difference statistical significance (P<0.01); The eye symptom integration of test group is reduced to 2.25 ± 1.86 after taking by taking 3.92 ± 1.94 before tested material, and front and back comparing difference has statistical significance (P<0.01); After test-meal, test group eye symptom integration and matched group (3.35 ± 1.85) comparing difference has statistical significance (P<0.01).Test group total effective rate (49.02%) higher than (3.92%) of matched group (P<0.05).Conclusion: test confirms that this health food has the effect of asthenopia releasing function to human body.

Claims

1. a blue berry xanthophyll soft capsule that improves vision, its composition comprises: capsule shells, in described capsule shells, there is content, it is characterized in that: described content comprises that parts by weight are the Pericarpium Citri tangerinae extract of 43-47, the zinc gluconate that parts by weight are 20-23, the vitamin C that parts by weight are 14-16, the phylloxanthin powder that parts by weight are 11-14, the vitamin E that parts by weight are 3-4, the vitamin A that parts by weight are 0.2-1, the soybean oil that parts by weight are 143-146, the peak wax that parts by weight are 6-9; Described capsule shells comprises that parts by weight are the gelatin of 190-210, the glycerol that parts by weight are 70-90, the purified water that parts by weight are 190-210, the burnt sugar coloring that parts by weight are 9-10.

2. the blue berry xanthophyll soft capsule that improves vision according to claim 1, is characterized in that: described content comprises that parts by weight are 43 Pericarpium Citri tangerinae extract, the zinc gluconate that parts by weight are 20, the vitamin C that parts by weight are 14, the phylloxanthin powder that parts by weight are 11, the vitamin E that parts by weight are 3, the vitamin A that parts by weight are 0.2, the soybean oil that parts by weight are 143, the peak wax that parts by weight are 6; Described capsule shells comprises that parts by weight are 190 gelatin, the glycerol that parts by weight are 70, the purified water that parts by weight are 190, the burnt sugar coloring that parts by weight are 9.

3. the blue berry xanthophyll soft capsule that improves vision according to claim 1, is characterized in that: described content comprises that parts by weight are 47 Pericarpium Citri tangerinae extract, the zinc gluconate that parts by weight are 23, the vitamin C that parts by weight are 16, the phylloxanthin powder that parts by weight are 14, the vitamin E that parts by weight are 4, the vitamin A that parts by weight are 1, the soybean oil that parts by weight are 146, the peak wax that parts by weight are 9; Described capsule shells comprises that parts by weight are 210 gelatin, the glycerol that parts by weight are 90, the purified water that parts by weight are 210, the burnt sugar coloring that parts by weight are 10.

4. the blue berry xanthophyll soft capsule that improves vision according to claim 1, it is characterized in that: described content comprises that parts by weight are 45 Pericarpium Citri tangerinae extract, the zinc gluconate that parts by weight are 21.5, the vitamin C that parts by weight are 15, the phylloxanthin powder that parts by weight are 12.5, the vitamin E that parts by weight are 3.3, the vitamin A that parts by weight are 0.6, the soybean oil that parts by weight are 144.5, the peak wax that parts by weight are 7.6; Described capsule shells comprises that parts by weight are 200 gelatin, the glycerol that parts by weight are 80, the purified water that parts by weight are 200, the burnt sugar coloring that parts by weight are 9.6.

5. a manufacture method for the blue berry xanthophyll soft capsule that improves vision described in claim 1-4, is characterized in that: the method comprises the steps:

(1) processing of raw material: sieve, weigh: Pericarpium Citri tangerinae extract, zinc gluconate, vitamin C, phylloxanthin powder are crossed respectively to 80 mesh sieves, by above-mentioned parts by weight, accurately weigh, standby;

(2) batching: by Pericarpium Citri tangerinae extract, zinc gluconate, vitamin C, phylloxanthin powder mix homogeneously 20min, obtain mixture; When soybean oil is heated to 75 ℃, add Cera Flava, stir melting 1h, obtain oily wax liquid, be cooled to 50 ℃ of insulations, stand-by; VitAVitE, mixture are added in oily wax liquid successively, and limit edged stirs, and 30min crosses colloid mill 2 ~ 3 times after mix homogeneously, and the de-bubble to the greatest extent of-0.06 ~-0.08Mpa, obtains content stand-by;

(4) pelleting: start soft capsule press, compacting soft gelatin capsule, 18 ~ 26 ℃ of production environment temperature, relative humidity 30 ~ 40%, controls loading amount 0.25g/ grain;

(5) sizing: the soft gelatin capsule suppressing is shaped in rotating cage with tape transport, 18 ~ 26 ℃ of temperature, relative humidity 30 ~ 40%, time 4h;

(6) wash ball, dry: with 95% edible ethanol, clean soft gelatin capsule, until soft gelatin capsule any surface finish, bright, without grease, is then sent into soft gelatin capsule hothouse dry, control 24 ~ 30 ℃ of hothouse temperature, relative humidity 20 ~ 30%, dry 24h;

(7) lamp is picked up: dried soft capsule is placed on lamp inspection machine, special messenger picks, conscientiously check soft capsule presentation quality, by empty pocket, special-shaped ball, flat ball, thin-walled ball, not of uniform sizely cause, ball surface is untidy, bonding, distortion, leak the defective works such as capsule and sort out;

(8) pressing plate: soft capsule is carried out to pressing plate, 10/plate, adopt the hard sheet of polrvinyl chloride solid medicinal.