CN104138403A - Drying method of radix tetrastigme - Google Patents
Drying method of radix tetrastigme Download PDFInfo
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- CN104138403A CN104138403A CN201410418719.7A CN201410418719A CN104138403A CN 104138403 A CN104138403 A CN 104138403A CN 201410418719 A CN201410418719 A CN 201410418719A CN 104138403 A CN104138403 A CN 104138403A
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- radix
- dry
- apioris fortunei
- lespedezae buergeri
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Abstract
The invention relates to a drying method of radix tetrastigme. The method comprises the following steps: freezing the fresh radix tetrastigme and then drying. The content of main active ingredients in the prepared radix tetrastigme is high, so that the anti-cancer effect is good.
Description
Invention field
The present invention relates to medicine method for making, particularly a kind of drying means of Radix Apioris Fortunei (Radix Lespedezae Buergeri), belongs to medical technical field.
Background technology
Radix Apioris Fortunei (Radix Lespedezae Buergeri) (Tetrastigmatis hemsleyani) is its tuber of Vitaceae Tetrastigma plant tetratigma hemsleyanum (Tetrastigma hemsleyanum Diels et Gilg) Radix Tetrastigmatis Hemsleyani by name, different name has stone monkey, Shi Baozi, blocks mountain tiger, thunder courage, tetrastigma hemsleyanum, soil through ball, search folder wind, SANYE and, little Caulis et Radix Tetrastigmatis Planicaulis, cloudy spirit, SANYE Caulis et Radix Tetrastigmatis Planicaulis, hemsley rockvine root, silk thread are hung to Jin Zhong, gold thread hoist, gold thread hang Rhizoma Solani tuber osi.Radix Apioris Fortunei (Radix Lespedezae Buergeri), with tuber or all herbal medicine, has the function of heat-clearing and toxic substances removing, dispelling pathogenic wind and eliminating phlegm, promoting blood circulation and stopping pain.It takes orally can treat children with high fever induced convulsions, dysentery, bronchitis, pneumonia, pharyngolaryngitis, hepatitis and viral meningitis, and external application can be treated venom, tonsillitis, cellulitis, traumatic injury.
Traditional Chinese medicine drying has very long history in China, the Chinese herbal medicine of drying can keep certain medicinal ingredient and be difficult for putrid and deteriorated, the dry important measures as ensureing Chinese herbal medicine quality, it is a requisite technical process in Chinese herbal medicine processing, it is in close relations that dry and Chinese medicine are produced, and dry method will directly affect the quality of product.
Patent of invention CN200910156906, discloses the technique that a kind of Chinese medicine Radix Apioris Fortunei (Radix Lespedezae Buergeri) vacuum lyophilization retains drug activity composition, comprises that Radix Apioris Fortunei (Radix Lespedezae Buergeri) raw material chooses, cleans discharging after section, predrying, sabot pre-freeze, sublimation drying, parsing-desiccation.The Radix Apioris Fortunei (Radix Lespedezae Buergeri) of preparation, can preserve the active ingredient of former medicine and the performance of going back crude drug in whole to greatest extent, and main antineoplastic component total flavones is 3.6 times of traditional diamond-making technique, and total Saponin is 2.5 times of traditional diamond-making technique.Shortcoming is that energy consumption is high, and decoction pieces is loose frangible, transport storage inconvenience.
Summary of the invention
The object of this invention is to provide a kind of drying means of Radix Apioris Fortunei (Radix Lespedezae Buergeri), the method is dried Radix Apioris Fortunei (Radix Lespedezae Buergeri), mainly contains effective component content high, and anticancer effect is good.
The object of the present invention is achieved like this:
A drying means for Radix Apioris Fortunei (Radix Lespedezae Buergeri), is characterized in that: get fresh Radix Apioris Fortunei (Radix Lespedezae Buergeri), and freezing rear dry.
The drying means of a kind of Radix Apioris Fortunei (Radix Lespedezae Buergeri) of the present invention, is characterized in that: freezing is to be refrigerated to freeze.
The drying means of a kind of Radix Apioris Fortunei (Radix Lespedezae Buergeri) of the present invention, is characterized in that: freezing is to be refrigerated to below-5 DEG C.
The drying means of a kind of Radix Apioris Fortunei (Radix Lespedezae Buergeri) of the present invention, is characterized in that: freezing rear dry be dry or dry or dry after freezing.
So far completed the present invention, Radix Apioris Fortunei (Radix Lespedezae Buergeri) main active content prepared by the present invention is high, and anticancer effect is good.
Further illustrate usefulness of the present invention below by test example, test example is intended to further illustrate usefulness of the present invention, but not limitation of the present invention.
To, with a collection of fresh Radix Apioris Fortunei (Radix Lespedezae Buergeri), process by the following method.
1) dry: fresh Radix Apioris Fortunei (Radix Lespedezae Buergeri), cut sheet, be put in shady and cool ventilation place, spread out and dry;
2) dry: fresh Radix Apioris Fortunei (Radix Lespedezae Buergeri), cut sheet, 60 DEG C of following oven dry;
3) freezing post-drying: fresh Radix Apioris Fortunei (Radix Lespedezae Buergeri), cut sheet, be refrigerated to-5 DEG C to freeze all, take out 60 DEG C of following oven dry.
One, the content side definite proportion of the dry Radix Apioris Fortunei (Radix Lespedezae Buergeri) total flavones of distinct methods
1, instrument and reagent
Instrument: the visible spectrophotometer of ultraviolet one; Electronic balance; Control substance of Rutin.
2, detect determining of wavelength
After the colour developing of control substance of Rutin aluminum nitrate, have absorption maximum at 500-510nm wavelength place, in conjunction with document, test and Selection 500nm is for detecting wavelength.
3, the preparation of reference substance solution
Precision takes the control substance of Rutin 10mg to constant weight at 120 DEG C of drying under reduced pressure, puts in 50mL measuring bottle, adds 70% ethanol 35mL, puts slight fever in water-bath and makes to dissolve, and lets cool, and adds 70% ethanol to scale, shakes up, and obtains (in every mL, containing anhydrous rutin 0.2mg).
4, test sample preparation and assay
Get the each 0.1g of Radix Apioris Fortunei (Radix Lespedezae Buergeri) medical material of above preparation, accurately weighed, put in l00mL volumetric flask, add 70% ethanol 70mL, after jolting, add ethanol to scale, place lh, filter rapidly with dry filter, precision measures subsequent filtrate 6mL, put in 25mL volumetric flask, add 5% sodium nitrite solution 1mL, mix, place 6min; Add 10% aluminum nitrate solution lmL, shake up, place 6min; Hydro-oxidation sodium test solution 10mL, then add water to scale, shake up, place 15min.
5, measure
Measure trap according to spectrophotography at 500nm wavelength place, calculate, to obtain final product.The results are shown in Table 1.
The content of the dry Radix Apioris Fortunei (Radix Lespedezae Buergeri) total flavones of table 1 distinct methods
| Medical material | Dry | Dry | Freezing post-drying |
| General flavone content (%) | 0.33 | 0.32 | 0.47 |
Found out by table 1, the freezing post-drying of Radix Apioris Fortunei (Radix Lespedezae Buergeri), general flavone content is all higher.
Two, the content side definite proportion of the dry Radix Apioris Fortunei (Radix Lespedezae Buergeri) total flavones of freezing rear distinct methods
To, with a collection of fresh Radix Apioris Fortunei (Radix Lespedezae Buergeri), cut sheet, be refrigerated to-10 DEG C to freeze all, take out, and dry respectively, dry, 60 DEG C of following oven dry.The content of measuring the dry Radix Apioris Fortunei (Radix Lespedezae Buergeri) total flavones of freezing rear distinct methods, the results are shown in Table 2.
The content of the dry Radix Apioris Fortunei (Radix Lespedezae Buergeri) total flavones of the freezing rear distinct methods of table 2
| Medical material | Dry | Dry | Freezing post-drying |
| General flavone content (%) | 0.47 | 0.46 | 0.47 |
Three, the dry Radix Apioris Fortunei (Radix Lespedezae Buergeri) active anticancer comparison of distinct methods
1, prepare Radix Apioris Fortunei (Radix Lespedezae Buergeri) extract get respectively dry, oven dry, freezing post-drying pulverize, and respectively decocts with water twice, adds water for the first time 8 times and measure, decoct 2 hours, add water for the second time 8 times and measure, decoct 1 hour, merge medicinal liquid, medicinal liquid filters, and gets filtrate, and being concentrated into relative density is 1.10(60 DEG C) time, add ethanol to make to reach 75% containing alcohol amount, precipitation, leaves standstill 36 hours, draw supernatant, reclaim ethanol, concentrated, dry, pulverize, obtain the dry Radix Apioris Fortunei (Radix Lespedezae Buergeri) extract of distinct methods.
2, tumor cell line people lung source adenocarcinoma cell SPCA-1, human hepatoma cell line HepG2 and people's chronic myelogenous leukemia cell K562, use RPMI-1649 culture fluid, and 37 DEG C, 5%CO2, relative humidity 100% is cultivated, and attached cell 0.25% trypsinization goes down to posterity.
3, the inhibitory action of method MTT colorimetric method for determining extract to growth of tumour cell.The trophophase cell of taking the logarithm, is mixed with cell suspension, 0.8 × 105/ml of 1 × 105/ml of suspension cell attached cell with fresh RPMI-1640 culture fluid.Suspension cell is inoculated in 96 well culture plates after adding respectively variable concentrations Experimental agents; Attached cell is first inoculated in 96 well culture plates, after every hole 90 μ l24h, adds respectively variable concentrations Experimental agents.Final volume is that every hole 100 μ l and every group are established 3 parallel holes, grouping: be subject to reagent group (culture fluid+cell suspension+variable concentrations is subject to reagent), negative control group (culture fluid+cell suspension), medicine color matched group (culture fluid+variable concentrations is subject to reagent), blank group (culture fluid+normal saline).Tested concentration is followed successively by 10 μ g/ml, 30 μ g/ml, 50 μ g/ml.Put 37 DEG C, in 5%CO2 incubator, cultivate after 72h, after adding the 10 μ l concussions of MTT solution to mix to every hole, continue to cultivate 4h, add SDS90 μ l to stop cultivating, 37 DEG C are spent the night, and then under room temperature, on micro oscillator, shake 10min, microplate reader is measured the absorbance (OD value) at 570nm wavelength place, and experiment repeats 3 times.
Calculate as follows growth inhibition ratio:
Growth inhibition ratio (%)=(N group OD average-B group OD average)-(T group OD average-TC group OD average)/N group OD average-B group OD average × 100%.
4, suppression ratio the results are shown in Table 3, table 4, table 5.
The suppression ratio result of table 3 to SPCA-1
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| Dry | 43.64 | 58.63 | 66.68 |
| Dry | 42.75 | 58.71 | 66.52 |
| Freezing post-drying | 51.35 | 64.38 | 81.64 |
The suppression ratio result of table 4 to HepG2
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| Dry | 46.59 | 50.74 | 61.20 |
| Dry | 47.06 | 50.63 | 62.14 |
| Freezing post-drying | 57.38 | 64.96 | 76.81 |
The suppression ratio result of table 5 to K562
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| Dry | 50.35 | 67.21 | 73.52 |
| Dry | 51.63 | 67.22 | 73.54 |
| Freezing post-drying | 67.37 | 78.41 | 86.59 |
Result shows, freezing post-drying Radix Apioris Fortunei (Radix Lespedezae Buergeri) all dries, dries Radix Apioris Fortunei (Radix Lespedezae Buergeri) to the propagation of SPCA-1, HepG2, K562 cell stronger inhibitory action.
Detailed description of the invention
embodiment 1
Fresh Radix Apioris Fortunei (Radix Lespedezae Buergeri), cuts sheet, is refrigerated to-5 DEG C to freeze all, takes out 60 DEG C of following oven dry.
embodiment 2
Fresh Radix Apioris Fortunei (Radix Lespedezae Buergeri), cuts sheet, is refrigerated to-10 DEG C to freeze all, takes out 60 DEG C of following oven dry.
embodiment 3
Fresh Radix Apioris Fortunei (Radix Lespedezae Buergeri), cuts sheet, is refrigerated to-20 DEG C to freeze all, takes out, and dries.
embodiment 4
Fresh Radix Apioris Fortunei (Radix Lespedezae Buergeri), cuts sheet, is refrigerated to-30 DEG C to freeze all, takes out, and dries.
embodiment 5
Fresh Radix Apioris Fortunei (Radix Lespedezae Buergeri), cuts sheet, is refrigerated to-15 DEG C to freeze all, takes out, and dries.
embodiment 6
Fresh Radix Apioris Fortunei (Radix Lespedezae Buergeri), cuts sheet, is refrigerated to-28 DEG C to freeze all, takes out, and dries.
Claims (4)
1. a drying means for Radix Apioris Fortunei (Radix Lespedezae Buergeri), is characterized in that: get fresh Radix Apioris Fortunei (Radix Lespedezae Buergeri), and freezing rear dry.
2. according to the drying means of a kind of Radix Apioris Fortunei (Radix Lespedezae Buergeri) of claim 1, it is characterized in that: freezing is to be refrigerated to freeze.
3. according to the drying means of a kind of Radix Apioris Fortunei (Radix Lespedezae Buergeri) of claim 1, it is characterized in that: freezing is to be refrigerated to below-5 DEG C.
4. according to the drying means of a kind of Radix Apioris Fortunei (Radix Lespedezae Buergeri) of claim 1, it is characterized in that: freezing rear dry be dry or dry or dry after freezing.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107080766A (en) * | 2017-03-28 | 2017-08-22 | 杭州本草中药有限公司 | Anti-liver cancer drug compositions containing radix tetrastigme general flavone and preparation method thereof |
| CN109373704A (en) * | 2016-07-26 | 2019-02-22 | 赵俊 | Radix tetrastigme drying means |
| CN109444275A (en) * | 2018-10-18 | 2019-03-08 | 浙江广胜药业有限公司 | A kind of method of rapid screening Chinese wistaria radix tetrastigme and sinomenium acutum radix tetrastigme medicinal material |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101791361A (en) * | 2009-12-24 | 2010-08-04 | 胡馨尹 | Vacuum freeze-drying process for retaining medicative active components of traditional Chinese medicine of radix tetrastigme |
| CN102846698A (en) * | 2012-08-20 | 2013-01-02 | 浙江中医药大学中药饮片有限公司 | Wall-broken radix tetrastigme oral decoction pieces |
| CN103749812A (en) * | 2013-12-24 | 2014-04-30 | 福建农林大学 | Processing method for radix tetrastigme tea |
-
2014
- 2014-08-25 CN CN201410418719.7A patent/CN104138403A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101791361A (en) * | 2009-12-24 | 2010-08-04 | 胡馨尹 | Vacuum freeze-drying process for retaining medicative active components of traditional Chinese medicine of radix tetrastigme |
| CN102846698A (en) * | 2012-08-20 | 2013-01-02 | 浙江中医药大学中药饮片有限公司 | Wall-broken radix tetrastigme oral decoction pieces |
| CN103749812A (en) * | 2013-12-24 | 2014-04-30 | 福建农林大学 | Processing method for radix tetrastigme tea |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109373704A (en) * | 2016-07-26 | 2019-02-22 | 赵俊 | Radix tetrastigme drying means |
| CN107080766A (en) * | 2017-03-28 | 2017-08-22 | 杭州本草中药有限公司 | Anti-liver cancer drug compositions containing radix tetrastigme general flavone and preparation method thereof |
| CN109444275A (en) * | 2018-10-18 | 2019-03-08 | 浙江广胜药业有限公司 | A kind of method of rapid screening Chinese wistaria radix tetrastigme and sinomenium acutum radix tetrastigme medicinal material |
| CN109444275B (en) * | 2018-10-18 | 2021-12-10 | 浙江广胜药业有限公司 | Method for rapidly screening medicinal materials of wisteria floribunda and sinomenium acutum |
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Application publication date: 20141112 |