CN104137732A - Method for improving cultivation quality of cordyceps millitaris - Google Patents
Method for improving cultivation quality of cordyceps millitaris Download PDFInfo
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- CN104137732A CN104137732A CN201310713801.8A CN201310713801A CN104137732A CN 104137732 A CN104137732 A CN 104137732A CN 201310713801 A CN201310713801 A CN 201310713801A CN 104137732 A CN104137732 A CN 104137732A
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- cordyceps
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- cordyceps militaris
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- millitaris
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Abstract
The invention relates to a method for improving cultivation quality of cordyceps millitaris. The method is applicable to operating techniques and processes for improving the quality of artificially cultivated cordyceps millitaris at the cultivation stage. The method is characterized in that a biological agent prepared through cordyceps millitaris extraction is injected into inoculated pupa bodies to induce fruiting bodies to be synthesized into cordyceps effective ingredients during primordium differentiation, so that the purpose of effectively increasing content and quality indexes of effective ingredients such as product cordycepin and the like is achieved. The method comprises the following steps to finish the technical operation: step one, extracting an ultraclean liquid with 10% cordyceps millitaris ultra-micro whole powder, and diluting the liquid for 5 times with distilled water to prepare an ultraclean aqueous solution; step two, at the primordium conversion stage of the cordyceps millitaris, injecting the cordyceps whole powder extracted ultraclean liquid into the pupa bodies which become stiff silkworms. Through the technical processing, the primordium differentiation number of the cordyceps millitaris is stable, the fruiting bodies are strong, the first-class rate is increased, and the product quality is improved.
Description
Denomination of invention: a kind of method that improves Cordyceps militaris cultivation quality
Technical field: the present invention relates to a kind of method that improves Cordyceps militaris training quality.Be applicable to artificial culture Cordyceps militaris cultivation stage and put forward high-quality operating technology and technique.
Technical background: in Cordyceps militaris cultivating process, existing cultivation flow process is at present: tussah silkworm chrysalis body sterilization, injection inoculation, dark cultivation, the differentiation of former base, light are cultivated, gathered.Make existing conventional cultivation under technical conditions, content 0.08% left and right of topmost functional component cordycepin in Cordyceps militaris.
Therefore, in the fruiting bodies of cordyceps militaris stage, promote former base differentiation, elicitor entity synthesizes Chinese caterpillar fungus active ingredient, becomes the key that ensures artificial culture Cordyceps militaris inherent quality.Also be the important step that improves producer's economic benefit.
Goal of the invention: innovate the method for the synthetic Chinese caterpillar fungus active ingredient of a kind of elicitor entity, improve artificial culture Cordyceps militaris inherent quality.
Summary of the invention: the present invention is in former base differentiation, to a kind of biologic product making that extracted by Cordyceps militaris of inoculation pupal cell injection; Reach the target of the quality index such as active constituent content such as effective raising product cordycepin.
Technical scheme of the present invention: the present invention is operated by following two step completing technologies.
The first step, utilizes the ultra-clean liquid of 10% Cordyceps militaris ultramicro whole powder lixiviate, with 5 times of distilled water dilutings, makes the ultra-clean aqueous solution, carries, filters through ultrasonic-wave assisted, makes the ultra-clean aqueous solution of the full powder lixiviate of Chinese caterpillar fungus, and the sealing of filtrate normal temperature is preserved.Used time gets filtrate and uses 5 times of uses of distilled water diluting.(as Fig. 1).
Second step, in the former base of the Cordyceps militaris transformation stage, carries out Ozone Water sterilization by the pupal cell that becomes bombys batryticatus, then carries out the ultra-clean liquid injection of the full powder lixiviate of pupal cell Chinese caterpillar fungus.In injection process, inject respectively the prefabricated Chinese caterpillar fungus Chinese caterpillar fungus ultra-clean aqueous solution of full powder lixiviate (as Fig. 2) of 0.01ml along six position degree of depth 3-4mm of silkworm chrysalis body both sides spiracle line.
Beneficial effect of the present invention is: by technical finesse of the present invention, make the former base quantity of differentiation of Cordyceps militaris stable, fruit body is strong, improves top-quality product rate, and the quality of product is improved.Facts have proved, Cordyceps militaris excellence rate can be brought up to 85-90% from conventional approximately 50% by processing of the present invention, cordycepin content is brought up to 0.12-0.18% by 0.08% left and right of traditional approach.Cultivate Cordyceps militaris cordycepin content 0.04-0.1 percentage point higher than domestic and foreign current Traditional Man, the factor of merit is higher than 35-40 percentage point.Realize the goal of the invention of setting.
Tool of the present invention has the following advantages:
1, can effectively improve the former base differentiation capability of Cordyceps militaris and improve excellence rate;
2, can effectively improve the content of the inherent active ingredient of product;
3, simple to operate, material is easy to get, convenient and easy;
4, derivant of the present invention is biologic product, is all absorbed conversion at Cordyceps militaris vegetative stage.
Brief description of the drawings:
Fig. 1. be: biologic product-derivant preparation schematic diagram;
In figure: being 1. Cordyceps militaris Ultramicro-powder, is 2. 10 times of distilled water, is 3. extraction vessel, is 4. ultrasonic extraction procedure, 5. filter, 6. Cordyceps militaris cleaner liquid
Fig. 2 is the map title: along pupal cell spiracle line point note derivant schematic diagram;
In figure: 1., 2., 3., 4., 5., be 6. same specification injection point.
Concrete enforcement example, and by reference to the accompanying drawings, the present invention is further described.
Embodiment: taking artificial culture cordyceps as example
First prepare cordycepin derivant liquid.The first step, get 20g Cordyceps militaris Ultramicro-powder (as Fig. 1 1.), be placed in ultrasonic extractor, add distilled water 200ml, (as Fig. 1 2.), ultrasonic extract 40 minutes (as Fig. 1 3.), utilize Filter paper filtering (as Fig. 1 4.), fixed molten 200 milliliters (as Fig. 1 5.), for subsequent use.
Second step, chooses the silkworm chrysalis that becomes bombys batryticatus (bodily form is plentiful, hand by good springiness) of growing way stalwartness.By the sterilization of bombys batryticatus pupal cell Ozone Water, implement injection inoculation, then take advantage of a situation and inject respectively the ultra-clean aqueous solution of the full powder lixiviate of prefabricated Chinese caterpillar fungus---the derivant solution (as Fig. 2) of 0.01ml along six position shallow tables of syringe needle of silkworm chrysalis spiracle line.
Thereby make the present invention effectively reach aforesaid goal of the invention and effect.
Claims (4)
1. improve a method for Cordyceps militaris training quality, comprise the ultra-clean liquid preparation of Cordyceps militaris ultramicro whole powder lixiviate and formula and operating technology and the method for carrying out the ultra-clean liquid injection of the full powder lixiviate of Chinese caterpillar fungus to becoming the pupal cell of bombys batryticatus.
2. as claimed in claim 1 is to utilize the ultra-clean liquid of 10% Cordyceps militaris ultramicro whole powder lixiviate for improving the ultra-clean liquid preparation of Cordyceps militaris ultramicro whole powder lixiviate and the formula of Cordyceps militaris training quality, with distilled water diluting, makes the ultra-clean aqueous solution of the full powder lixiviate of Chinese caterpillar fungus.
3. a kind of method that improves Cordyceps militaris training quality as described in claim 1,2 is that the ultra-clean aqueous solution of full Chinese caterpillar fungus powder lixiviate is expelled to and becomes six positions of spiracle line, the pupal cell of bombys batryticatus both sides.
4. the present invention includes but be not limited to a kind of method that improves Cordyceps militaris training quality of this claim, comprise the ultra-clean liquid preparation of Cordyceps militaris ultramicro whole powder lixiviate and formula and carry out the operating technology of the ultra-clean liquid injection of the full powder lixiviate of Chinese caterpillar fungus and the embodiment of method to becoming the pupal cell of bombys batryticatus, as long as in essential scope of the present invention, variation, modification that above-described embodiment is done all will belong within the scope of claim of the present invention.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310713801.8A CN104137732A (en) | 2013-12-23 | 2013-12-23 | Method for improving cultivation quality of cordyceps millitaris |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310713801.8A CN104137732A (en) | 2013-12-23 | 2013-12-23 | Method for improving cultivation quality of cordyceps millitaris |
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| CN104137732A true CN104137732A (en) | 2014-11-12 |
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| CN201310713801.8A Pending CN104137732A (en) | 2013-12-23 | 2013-12-23 | Method for improving cultivation quality of cordyceps millitaris |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005287488A (en) * | 2004-04-03 | 2005-10-20 | Ryoto Bussan:Kk | Culture medium for cordyceps sinensis incorporated with ant powder and extract |
| CN1754548A (en) * | 2004-09-29 | 2006-04-05 | 李义海 | Chinese caterpillar fungus injection preparation method |
| CN102037856A (en) * | 2009-10-16 | 2011-05-04 | 北京农业生物技术研究中心 | Simple cordyceps militaris strain rejuvenation method |
| CN103371056A (en) * | 2013-07-19 | 2013-10-30 | 鹤山市中春生物科技有限公司 | Cultivation method of Cordyceps militaris (pupa) |
-
2013
- 2013-12-23 CN CN201310713801.8A patent/CN104137732A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005287488A (en) * | 2004-04-03 | 2005-10-20 | Ryoto Bussan:Kk | Culture medium for cordyceps sinensis incorporated with ant powder and extract |
| CN1754548A (en) * | 2004-09-29 | 2006-04-05 | 李义海 | Chinese caterpillar fungus injection preparation method |
| CN102037856A (en) * | 2009-10-16 | 2011-05-04 | 北京农业生物技术研究中心 | Simple cordyceps militaris strain rejuvenation method |
| CN103371056A (en) * | 2013-07-19 | 2013-10-30 | 鹤山市中春生物科技有限公司 | Cultivation method of Cordyceps militaris (pupa) |
Non-Patent Citations (2)
| Title |
|---|
| 刘晓红: "柞蚕蛹虫草栽培试验研究", 《食用菌》, no. 1, 31 January 2011 (2011-01-31), pages 39 - 40 * |
| 秦秀丽等: "北冬虫夏草寄主体培养关键技术研究", 《北方园艺》, no. 17, 30 September 2013 (2013-09-30), pages 157 - 160 * |
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Application publication date: 20141112 |