CN104131086A - SSR labeled fingerprint of flammulina velutipes F3 strain and applications thereof - Google Patents
SSR labeled fingerprint of flammulina velutipes F3 strain and applications thereof Download PDFInfo
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- CN104131086A CN104131086A CN201410318267.5A CN201410318267A CN104131086A CN 104131086 A CN104131086 A CN 104131086A CN 201410318267 A CN201410318267 A CN 201410318267A CN 104131086 A CN104131086 A CN 104131086A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Abstract
The invention relates to a SSR label fingerprint of a flammulina velutipes F3 strain and applications thereof. The fingerprint is composed of six pairs of SSR labeled specific allelic sections which are developed on the basis of the genomic sequence of flammulina velutipes. The fingerprint can be used to identify the flammulina velutipes F3 strain, and the identification comprises the following steps: carrying out SSR labeling amplification on a flammulina velutipes strain, and then comparing the obtained fingerprint with the fingerprint of flammulina velutipes F3 strain, if the two fingerprints are identical, the flammulina velutipes strain is the F3 strain. Compared to the conventional morphology detection, antagonism tests, and fruiting experiments, the fingerprint identification method has the advantages of short detection time, high accuracy, and good repeatability, and has the specificity on F3 strain in collected 24 flammulina velutipes strains which are commonly cultured in China.
Description
Technical field
The invention belongs to the detection field of needle mushroom bacterial classification, particularly a kind of SSR marking fingerprint and application of needle mushroom F3 bacterial classification.
Background technology
Needle mushroom [Flammulina velutipes (Fr.) Sing.] has another name called golden mushroom, plain mushroom, structure bacterium, QINGKANGJUN, hair handle money bacterium, in classification, belongs to Agaricales Bai Mo section flammule Pseudomonas.Needle mushroom is widely distributed, nutritious, is edible mushrooms and medicinal fungus that a kind of economic worth is very high, is also one of natural health care extremely selling well on market.Needle mushroom is that Chinese Main Cultivation edible mushrooms and modern factoriesization are produced one of Pollution In Edible Mushrooms, and within 2013, domestic batch production day output reaches 2700 tons.
The contribution rate of good quality strain in needle mushroom per unit area yield and quality is very important, and this has determined the critical role of needle mushroom bacterial classification in needle mushroom industry.Within 1999, China has signed the international new variety of plant protection method of < < > >; this not only requires us to respect other national kind intellecture property; also want the kind intellecture property of our country oneself of more protection simultaneously; in order to set up edible mushrooms new variety resignation system, really protect the kind property right of China; the cultivar identification technology of necessary model maturation, for new variety registration lays the foundation.Especially Japan comes into effect < < seedling method amendment > > on April 1st, 2004, and the production of China edible mushrooms especially needle mushroom has been formed to great threat.With regard to domestic, the phenomenon of golden mushroom plantation bacterial classification " different name of the same race, xenogenesis is of the same name ", has brought great loss not only to mushroom agriculture, has affected their cultivation enthusiasm, has also greatly affected the development of Chinese needle mushroom industry; And, along with the appearance of large-scale factory culture mode, more and more higher to the requirement of golden mushroom plantation bacterial strain quality, need that development is more easy, identification of strains technology fast and accurately, with guarantee every batch with kind all accurate.
In the face of this situation, just need us further to accelerate the research of strain identification technology, in the research of needle mushroom, set up more efficiently strain identification system.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of SSR marking fingerprint and construction process and application of needle mushroom F3 bacterial classification, this finger printing is compared with conventional morphologic detection, antagonistic effect, fruiting experiment, have detection time short, accuracy is high, reproducible advantage.
The SSR marking fingerprint of a kind of needle mushroom F3 bacterial classification of the present invention, this finger printing is comprised of 6 pairs of SSR marks, is based on needle mushroom genome simple repeated sequence fragment SSR primers development, and amplification banding pattern is good, the SSR primer that repeatability is high, mark details are as table 1.
The list of table 1 SSR mark details
The application of the SSR marking fingerprint of a kind of needle mushroom F3 bacterial classification of the present invention, 6 pairs of SSR primers that utilize the exploitation of needle mushroom genome simple repeated sequence fragment, by the SSR primer banding pattern of 24 parts of domestic main golden mushroom plantation kinds of collecting is increased, the present invention has determined quantity the numbering (table 2) of the allele that 6 pairs of SSR primers amplify in needle mushroom F3 bacterial classification, by the numbering combination of different SSR alleles, can effectively identify F3 bacterial classification (Fig. 2-7).By contrast 50bp DNAladder, can determine the relative molecular weight of the allele of each SSR primer amplification, there is the bacterial classification of the special SSR allele combination of F3 bacterial classification, be needle mushroom F3 bacterial classification, the numbering of this bacterial classification is combined as: (1+2) 12 (1+2) (1+2+3+4) (1+3).
The allele information summary sheet of table 2SSR primer amplification
beneficial effect
The present invention compares with conventional morphologic detection, antagonistic effect, fruiting experiment, has detection time short, and accuracy is high, reproducible advantage.Detecting required time only needs 3～4 days, and conventional antagonistic effect required time at least needs two time-of-weeks, and fruiting experiment needs the time of at least 2 months; The method has the specificity of F3 bacterial classification in the main cultivation bacterial classification of 24 needle mushroom of collected China, has a good application prospect.
Accompanying drawing explanation
Fig. 1 is needle mushroom F3 bacterial classification SSR marking fingerprint, and wherein M is 50bp DNA ladder, digitized representation be 6 pairs of SSR primers used, C is blank, arrow refers to the stable and special SSR allele combination of needle mushroom F3 bacterial classification;
Fig. 2 is the amplification collection of illustrative plates of primers F v_fp0001 in selected golden mushroom plantation material;
Fig. 3 is the amplification collection of illustrative plates of primers F v_fp0002 in selected golden mushroom plantation material;
Fig. 4 is the amplification collection of illustrative plates of primers F v_fp0003 in selected golden mushroom plantation material;
Fig. 5 is the amplification collection of illustrative plates of primers F v_fp0004 in selected golden mushroom plantation material;
Fig. 6 is the amplification collection of illustrative plates of primers F v_fp0005 in selected golden mushroom plantation material;
Fig. 7 is the amplification collection of illustrative plates of primers F v_fp0006 in selected golden mushroom plantation material.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment are only not used in and limit the scope of the invention for the present invention is described.In addition should be understood that those skilled in the art can make various changes or modifications the present invention after having read the content of the present invention's instruction, these equivalent form of values fall within the application's appended claims limited range equally.
Embodiment 1
(1) mycelium culture: needle mushroom bacterial classification is transferred in potato glucose substratum, and lucifuge shake-flask culture at 19 ℃～21 ℃, collects mycelia after 3d～4d;
(2) extraction of genomic dna: extract the genomic dna of above-mentioned mycelia by CTAB (cetyl trimethylammonium bromide) method, ultraviolet spectrophotometry detects total genomic dna concentration and purity, and the concentration of adjusting sample DNA is consistent;
The genomic dna technique that CTAB method is extracted mycelia comprises:
1. by the needle mushroom mycelia grind into powder of liquid-nitrogen freeze drying, add 2 * CTAB extract of 65 ℃ of preheatings, in 65 ℃ insulation 45～60min, or jog mix;
2. 12000rpm, 4 ℃ of centrifugal 20min, get supernatant liquor;
3. in above-mentioned supernatant liquor, adding volume ratio is the mixed solution of phenol, chloroform and the primary isoamyl alcohol of 25:24:1, mixes gently 10min, and 12000rpm, 4 ℃ of centrifugal 10min get supernatant liquor and move in centrifuge tube;
4. in above-mentioned centrifuge tube, adding volume ratio is chloroform and the primary isoamyl alcohol mixed solution of 24:1, mixes gently 10min, and 12000rpm, 4 ℃ of centrifugal 10min get supernatant liquor and move in another centrifuge tube;
5. the Virahol that adds-20 ℃ of precoolings of 2/3 volume (with respect to the supernatant liquor in step (4)) in above-mentioned centrifuge tube, shakes 5min gently, and 8000rpm, 4 ℃ of centrifugal 10min, remove supernatant;
6. the precipitation obtaining is washed 2～3 times to each 8000rpm, the centrifugal 5min of room temperature with 75vol% ethanol and 10mmol/L Potassium ethanoate;
7. the 95vol% ethanol that adds precooling, turns upside down gently, and the centrifugal 10min of 8000rpm room temperature, abandons ethanol, and vacuum is drained or naturally dried;
8. add 100 μ L10 * TE (Tris/EDTA) damping fluids, beat gently and make resolution of precipitate;
9. add the RNaseA of 1 μ L10mg/mL in 37 ℃ of water-bath 1h, remove RNA;
10. the DNA extraction thing obtaining is standby in-20 ℃ of refrigerator storages;
(3) detection of SSR molecule marker: the pcr amplification that the DNA of said extracted is carried out to gene SSR mark;
Pcr amplification system is: cumulative volume 20 μ L, comprising: 10 * PCR buffer2 μ L, 25mmol/L MgCl
22 μ L, 10mmol/LdNTP0.4 μ L, 5U/ μ L Taq DNA enzyme 0.2 μ L, each 1.5 μ L of 10 μ mol/L SSR mark forward primers and reverse primer cumulative volume, the template DNA 2 μ L that concentration 20ng～30ng/ μ L extracts, ddH
2o10.4 μ L;
PCR reaction conditions: 94 ℃ of 5min; 94 ℃ of 30second, 55 ℃ of 30second, 72 ℃ of 30second, 30 circulations; 72 ℃ of 5min.
(4) electrophoresis detection: the product that above-mentioned pcr amplification is obtained and 12 μ L sample loading buffers mix, in 95 ℃ of sex change 5min, finishes to be placed on cooling 3min in mixture of ice and water, point sample 3 μ L electrophoresis on denaturing polyacrylamide gel, the concentration of volume percent of denaturing polyacrylamide gel is 6%, electrophoretic buffer is 1 * TBE, voltage 1000v, electric current 200mA, power 200W, electrophoresis 45min, silver dyes, colour developing, takes pictures, analytical results.
The concrete technology that silver dyes is to unload and take apart sheet glass after electrophoresis completes, after offset plate unloads, with deionized water, wash 5-8 second, after draining away the water, in silver-colored dye liquor (1.5 grams Silver Nitrate and 1500ml water), lucifuge silver dyes 8 minutes, after silver dyes, with deionized water, wash 5-8 second, drain away the water, put into developing solution (16 grams of sodium hydroxide, 1000ml water and 8ml formaldehyde), be readable to the rear taking-up washing of developing.
Adopt 6 pairs of SSR primer pair Strains of Flammulina velutipes to carry out pcr amplification, by contrast 50bp DNA ladder, can determine quantity and the relative molecular weight of the allele of each SSR primer amplification, find and meet numbering and be combined as: (1+2) (1+2+3+4) bacterial classification of (1+3) of 12 (1+2), can determine that this bacterial classification is needle mushroom F3 bacterial classification, as shown in arrow mark in Fig. 1.For the accuracy that guarantees to identify, suggestion carries out repeating for three times experiment.
Claims (3)
1. the SSR marking fingerprint of a needle mushroom F3 bacterial classification, it is characterized in that: this finger printing is combined by the special allele of 6 pairs of SSR marks based on the exploitation of needle mushroom genome sequence, and 6 couples of SSR are marked at the allele information increasing on needle mushroom F3 bacterial classification and are specially:
The quantity of the allele of Fv_fp0001 is 2, and allele 1 is 700bp with respect to the molecular weight of 50bp DNA ladder; Allele 2 is 680bp with respect to the molecular weight of 50bp DNA ladder;
The quantity 1 of the allele of Fv_fp0002, allele 1 is 1300bp with respect to the molecular weight of 50bp DNA ladder,
The quantity of the allele of Fv_fp0003 is 1, and allele 2 is 680bp with respect to the molecular weight of 50bp DNA ladder;
The quantity 2 of the allele of Fv_fp0004, allele 1 is 1250bp with respect to the molecular weight of 50bp DNA ladder; Allele 2 is 1200bp with respect to the molecular weight of 50bp DNA ladder;
The quantity 4 of the allele of Fv_fp0005, allele 1 is 1100bp with respect to the molecular weight of 50bp DNA ladder, allele 2 is 800bp with respect to the molecular weight of 50bp DNA ladder; Allele 3 is 530bp with respect to the molecular weight of 50bp DNA ladder; Allele 4 is 500bp with respect to the molecular weight of 50bp DNA ladder;
The quantity of the allele of Fv_fp0006 is 2, and allele 1 is 400bp with respect to the molecular weight of 50bp DNA ladder; Allele 3 is 360bp with respect to the molecular weight of 50bp DNA ladder.
2. the SSR marking fingerprint of a kind of needle mushroom F3 bacterial classification according to claim 1, is characterized in that: the special numbering of described 6 pairs of SSR mark alleles is combined as: (1+2) 12 (1+2) (1+2+3+4) (1+3).
3. an application for the SSR marking fingerprint of needle mushroom F3 bacterial classification as claimed in claim 1, is characterized in that: be applied to identify the special allelic variation combination of needle mushroom F3 bacterial classification and identify that needle mushroom F3 bacterial classification is with respect to the specificity of the careful Varieties in Flammulina velutipes of its other country.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105316329A (en) * | 2015-11-20 | 2016-02-10 | 中国科学院昆明植物研究所 | Flammulina velutipes SSR molecular marker and corresponding primers and application thereof |
| CN105506124A (en) * | 2016-01-12 | 2016-04-20 | 浙江省农业科学院 | SSR labeling primer of flammulina velutipes F101 culture and fingerprint application thereof |
| KR20160106809A (en) * | 2015-03-02 | 2016-09-13 | 경상대학교산학협력단 | Novel genomic marker for identifying Flammulina vehitipes and uses thereof |
| CN106754978A (en) * | 2016-12-26 | 2017-05-31 | 上海市农业科学院 | A kind of specific molecular marker of bacterial strains of asparagus NG1 92 and its preparation method and application |
| CN112813193A (en) * | 2021-04-09 | 2021-05-18 | 上海市农业科学院 | Identification method of microsatellite DNA marker fingerprint of flammulina velutipes X995 strain and construction method and application thereof |
| CN112961934A (en) * | 2021-04-09 | 2021-06-15 | 上海市农业科学院 | Identification method of microsatellite DNA marker fingerprint of needle mushroom SCY12 strain, construction method and application thereof |
| CN112980994A (en) * | 2021-04-09 | 2021-06-18 | 上海市农业科学院 | Identification method of SSR marker fingerprint of needle mushroom strain and construction method and application thereof |
| CN112980995A (en) * | 2021-04-09 | 2021-06-18 | 上海市农业科学院 | Identification method of microsatellite DNA marker fingerprint of golden 1754 strain of needle mushroom as well as construction method and application of identification method |
| CN113151548A (en) * | 2021-04-08 | 2021-07-23 | 上海市农业科学院 | Identification method of microsatellite DNA marker fingerprint of flammulina velutipes FV1923 strain and construction method and application thereof |
-
2014
- 2014-07-04 CN CN201410318267.5A patent/CN104131086B/en active Active
Non-Patent Citations (3)
| Title |
|---|
| RUIYING ZHANG ET AL.: "Development and characterization of simple sequence repeat (SSR) markers for the mushroom Flammulina velutipes", 《JOURNAL OF BIOSCIENCE AND BIOENGINEERING》, vol. 110, no. 3, 2 May 2010 (2010-05-02), pages 273 - 275, XP027228216 * |
| 张瑞颖等: "食用菌SSR序列开发策略研究进展", 《菌物研究》, vol. 8, no. 4, 31 December 2010 (2010-12-31), pages 239 - 244 * |
| 杨成香等: "金针菇遗传多样性初步分析", 《中国食用菌》, vol. 26, no. 4, 31 December 2007 (2007-12-31), pages 37 - 39 * |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20160106809A (en) * | 2015-03-02 | 2016-09-13 | 경상대학교산학협력단 | Novel genomic marker for identifying Flammulina vehitipes and uses thereof |
| KR101684069B1 (en) | 2015-03-02 | 2016-12-08 | 경상대학교산학협력단 | Novel genomic marker for identifying Flammulina vehitipes and uses thereof |
| CN105316329B (en) * | 2015-11-20 | 2018-09-11 | 中国科学院昆明植物研究所 | Needle mushroom SSR molecular marker and its corresponding primer and application |
| CN105316329A (en) * | 2015-11-20 | 2016-02-10 | 中国科学院昆明植物研究所 | Flammulina velutipes SSR molecular marker and corresponding primers and application thereof |
| CN105506124B (en) * | 2016-01-12 | 2019-05-31 | 浙江省农业科学院 | The SSR label primer and its finger-print application of needle mushroom F101 strain |
| CN105506124A (en) * | 2016-01-12 | 2016-04-20 | 浙江省农业科学院 | SSR labeling primer of flammulina velutipes F101 culture and fingerprint application thereof |
| CN106754978A (en) * | 2016-12-26 | 2017-05-31 | 上海市农业科学院 | A kind of specific molecular marker of bacterial strains of asparagus NG1 92 and its preparation method and application |
| CN113151548A (en) * | 2021-04-08 | 2021-07-23 | 上海市农业科学院 | Identification method of microsatellite DNA marker fingerprint of flammulina velutipes FV1923 strain and construction method and application thereof |
| CN112813193A (en) * | 2021-04-09 | 2021-05-18 | 上海市农业科学院 | Identification method of microsatellite DNA marker fingerprint of flammulina velutipes X995 strain and construction method and application thereof |
| CN112961934A (en) * | 2021-04-09 | 2021-06-15 | 上海市农业科学院 | Identification method of microsatellite DNA marker fingerprint of needle mushroom SCY12 strain, construction method and application thereof |
| CN112980994A (en) * | 2021-04-09 | 2021-06-18 | 上海市农业科学院 | Identification method of SSR marker fingerprint of needle mushroom strain and construction method and application thereof |
| CN112980995A (en) * | 2021-04-09 | 2021-06-18 | 上海市农业科学院 | Identification method of microsatellite DNA marker fingerprint of golden 1754 strain of needle mushroom as well as construction method and application of identification method |
| CN112961934B (en) * | 2021-04-09 | 2023-02-28 | 上海市农业科学院 | Identification method of microsatellite DNA marker fingerprint of flammulina velutipes SCY12 strain and construction method and application thereof |
| CN112980995B (en) * | 2021-04-09 | 2023-02-28 | 上海市农业科学院 | Identification method of microsatellite DNA marker fingerprint of golden needle mushroom 1754 strain and construction method and application thereof |
| CN112813193B (en) * | 2021-04-09 | 2023-02-28 | 上海市农业科学院 | Identification method of microsatellite DNA marker fingerprint of flammulina velutipes X995 strain and construction method and application thereof |
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