CN104109700A - Method of producing monacolin K functional red yeast rice by solid-liquid co-fermentation - Google Patents
Method of producing monacolin K functional red yeast rice by solid-liquid co-fermentation Download PDFInfo
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Abstract
The invention discloses a method of producing monacolin K functional red yeast rice by solid-liquid co-fermentation and belongs to the field of fermentation engineering. The method disclosed by the invention comprises the following steps: firstly, carrying out a certain dry heat treatment process on a solid matrix, wherein the purposes of realizing a certain stuffing degree of materials, reducing the water content and sterilizing can be realized; then, uniformly mixing fermentation liquor with the treated solid matrix in a weight ratio of 1 to (0.5-5), and further fermenting for 10-20 days under a certain condition; and after fermentation, drying and smashing the solid matrix to obtain a red yeast rice powder product rich in monacolin K. The method can greatly improve the output of monacolin K compared with that of pure liquid fermentation and avoids the complex process of extracting and purifying later-stage products in liquid fermentation. Compared with pure solid fermentation, the fermentation time can be shortened and the utilization ratio of solid raw materials can be improved. Physiological active matters produced by the method can be applied in the fields such as health-care foods and medicines and the like in a large area.
Description
Technical field
The present invention relates to a kind of method that mushy stage combined ferment produces Monacolin K functional Monascus, belong to field of fermentation engineering.
Background technology
Monacolin K is the competitive inhibitor of the light methylpent two phthalein CoA-reductases of the biosynthetic key enzyme of body's cholesterol (HMG mono-CoA reductase enzyme), can effectively reduce the level of low density cholesterol (LDL) in human body.At present utilize the method for Monascus anka Nakazawa et sato fermentative Production Monacolin K to mainly contain liquid fermentation method and solid state fermentation both at home and abroad.
Liquid fermentation method is to carry out in ventilating machinary fermentor tank, artificially Optimal Medium and culture condition.Liquid fermentation method can utilize the feature that plant capacity is large to carry out scale operation, and human cost is low, level of automation advantages of higher, but liquid state fermentation has high input with respect to solid state fermentation equipment, power consumption is large, and aseptic requirement is stricter, and aftertreatment technology is more loaded down with trivial details.And at present liquid state fermentation is lower containing the fermentation level of Monacolin K, do not reach the requirement of suitability for industrialized production and unrealized industrialized production.
Solid state fermentation is taking cereal as raw material, and the solid state substrate by strain transfer to sterilizing, through solid state fermentation, dries pulverizing and obtain the finished product.The monascus product that this method obtains claims Hongqu powder (red colouring agent), can be used as protective foods or medicine for hyperlipidemia crowd.Solid state fermentation has equipment investment little, and less energy consumption asepticly requires lowly, and fermentation aftertreatment technology is easy, and the content of Monacolin K is higher.But be not suitable for the special solid-state fermentation reactor of functional Monascus at present.And solid-state fermentation process exists the solid state fermentation time long, easily microbiological contamination, labour intensity is large, manpower consumption is many, aftertreatment technology is relatively easy, be difficult to the shortcomings such as scale operation.
Create a kind of solid-state-liquid combined ferment law system, together with just the advantage of solid state fermentation and liquid state fermentation can being combined, thereby solve liquid state or the intrinsic difficult problem of solid state fermentation system, thereby realize the functional Monascus that can be mass-produced, improve production level and efficiency, increase the object of the performance of enterprises.
But liquid and solid-state combined ferment method is not that liquid state and solid-state two kinds of materials are combined and fermented simply.Because when solid state fermentation is produced functional Monascus, very strict to the requirement of moisture content of material.Too high or too low all not all right.The solid state fermentation of conventional functional Monascus, the water content of material is generally higher, if liquid state fermentation liquid and this solid fermented material are mixed, by making, follow-up material water content of substrate is higher again, causes follow-up fermentation cannot normally carry out and lead to the failure.Therefore need to have special method to process solid feed, its contained moisture content is declined greatly, reach again the requirement of raw material sterilizing simultaneously.
Solid state fermentation raw material of the present invention is through dry heat treatment, the structure of the materials such as raw starch changed and reach enough gelatinization degrees, can carry out effective sterilizing to raw material simultaneously.Be dry heat treatment method due to what adopt, and do not adopt traditional moist heat sterilization method, can make the water content of solid-state fermentation substrate lower, so just can increase substantially the access amount of liquid state fermentation liquid.In the situation that Monascus anka Nakazawa et sato inoculum size is large, Monascus anka Nakazawa et sato can Fast Growth, once Monascus anka Nakazawa et sato forms growth vigor, can effectively avoid the pollution of miscellaneous bacteria, for the follow-up solid state fermentation that carries out safely provides safeguard, finally reach the object that improves Monacolin K fermentation level.
Summary of the invention
A kind of method that the technical problem to be solved in the present invention is to provide mushy stage combined ferment produces Monacolin K functional Monascus, especially a kind of Monascus anka Nakazawa et sato liquid state-solid-state integrated process fermentative production is containing the method for the functional Monascus of Monacolin K.
Technical solution of the present invention mainly comprises the following steps:
(1) solid feed is carried out to dry heating method pre-treatment, reach not only sterilizing but also reduce the object of moisture content of material;
(2) cultivate and obtain Monascus anka Nakazawa et sato seed liquor;
(3) Monascus anka Nakazawa et sato liquid state fermentation in early stage;
(4) fermented liquid is accessed in solid feed, proceed later stage solid State Fermentation of Monascus;
The fermentation aftertreatments such as described scheme also comprises after solid state fermentation finishes dries, pulverizing.
The granular cheap cereal such as the preferred barley of described solid feed, wheat, rice, millet, corn or Chinese sorghum.
Described step (1) carries out to solid feed that dry heating method pre-treatment is preferably cured cereal materials through sunlight exposure, Infrared Heating, microwave treatment, high temperature or the mode processing such as high pressure vapour is quick-fried, make material moisture be down to 0.5%-5% and obtain solid-state fermentation substrate, sterilising effect is good, and sealing is preserved stand-by.
The preferred 1500-2000W/m of described sunlight exposure treatment condition
2intensity of sunshine is processed 1-4h, shakeouts thickness and is no more than 2cm.
100-200 DEG C of processing 0.5-10min of preferred infrared wavelength 75～1000 μ m heating of described Infrared Heating.
The preferred 800-1500W of described microwave treatment processes 0.5-5min, and treatment capacity is 0.5-2kg.
Described high temperature cures preferred 100-200 DEG C and processes 0.5-2h.
The quick-fried preferred 1.0-1.8MPa of described high pressure vapour, maintains 3-10min air pressure and die-offs, and dries to water content 0.5%-10%.
The monascus purpureus that the preferred deposit number of described step (2) red colouring agent for food, also used as a Chinese medicine bacterial classification is CGMCC3.4577, Monascus anka Nakazawa et sato is the conventional bacterial classification of foodstuffs industry.
Described step (2) Monascus anka Nakazawa et sato seed liquor be by Monascus anka Nakazawa et sato after PDA slant medium activation culture maturation, prepare spore suspension, wherein the concentration of spore suspension miospore is not less than 10
7individual/ml.By in spore suspension 10% ratio access by volume seed culture medium, 30 DEG C of 180r/min aerlbic cultures obtain seed liquor for 2 days.
Described seed culture medium is (1L) preferably: glucose 60g, peptone 25g, corn steep liquor 10mL, NaNO
32g, K
2hPO
43H
2o1g, MgSO
40.5g.
When described step (3) Monascus anka Nakazawa et sato liquid state fermentation in early stage, first by liquid state fermentation substratum according to formulated, after sterilizing, in 10-20% (V/V) ratio, cultured Monascus anka Nakazawa et sato seed liquor is accessed in liquid state fermentation substratum, 32 DEG C, 180r/min are cultivated 2 days, and then 28 DEG C, 180r/min are cultivated 8-18 days.Further preferably 32 DEG C, 180r/min are cultivated 2 days, and then 28 DEG C, 180r/min are cultivated 16 days.
Described liquid state fermentation medium optimization: glucose 20gL
-1, Soy hydrolysate 50mLL
-1, NaNO
32gL
-1, MgSO
47H
2o1gL
-1, K
2hPO
43H
2o1gL
-1, corn steep liquor 10mLL
-1, pH nature.
Described step (4) solid state fermentation is according to solid-state fermentation substrate and liquid state fermentation liquid mass ratio 1:(0.5-2 by liquid state fermentation liquid) (W/W) be transferred to solid-state fermentation substrate, 25-32 DEG C ferments 2 days, then 20-30 DEG C of fermentation 6-20 days, every 48h stirs once.
Described step (4) is further preferred: by liquid state fermentation liquid according to solid-state fermentation substrate and liquid state fermentation liquid mass ratio 1:(1-1.3) (W/W) be transferred to solid-state fermentation substrate, 28-32 DEG C ferments 2 days, then 24-28 DEG C of fermentation 8-16 days, every 48h stirs once.
Described step (4) is also further preferably: by liquid state fermentation liquid according to solid-state fermentation substrate and liquid state fermentation liquid mass ratio 1:(1-1.3) (W/W) be transferred to solid-state fermentation substrate, 30 DEG C ferment 2 days, then 25 DEG C ferment 12 days, and every 48h stirs once.
After described step (4) fermentation ends, by high-temperature sample sterilizing, then about 50 DEG C oven dry, pulverize.
Solid feed of the present invention adopts dry heat treatment method, makes the water content of solid-state fermentation substrate lower, can increase substantially the access amount of liquid state fermentation liquid.In the situation of solid-state material through dry heat treatment and a large amount of switchings of Monascus anka Nakazawa et sato, effectively avoid the pollution of miscellaneous bacteria, for the follow-up solid state fermentation that carries out safely provides safeguard.Solid state substrate treatment process of the present invention makes liquid state-solid-state combined ferment become possibility, has given full play to the advantages such as liquid state fermentation automatization in early stage is high, mass-producing is large, and the later stage proceeds to solid state fermentation and avoided the loaded down with trivial details last handling process of liquid state fermentation.Liquid state fermentation liquid Monacolin K content 350-500mg/kg during than access, improve more than 3 times through solid state fermentation Monacolin K content, also retained conventional solid-state fermentation monascus product color and luster pleasant, healthy and safe, the advantages such as applied range simultaneously.With conventional solid-state, fermentation needs 20 days above fermentation times to compare, and the present invention shortens the time that solid state fermentation is produced, and reduces the input of manufacturer's fermentation production process.
Embodiment
Seed culture medium (1L): glucose 60g, peptone 25g, corn steep liquor 10mL, NaNO
32g, K
2hPO
43H
2o1g, MgSO
40.5g.
Liquid state fermentation substratum: glucose 20gL
-1, Soy hydrolysate 50mLL
-1, NaNO
32gL
-1, MgSO
47H
2o1gL
-1, K
2hPO
43H
2o1gL
-1, corn steep liquor 10mLL
-1, pH nature.
The measuring method of Monacolin K content: get liquid state fermentation liquid 10ml or solid-state red kojic rice powder 1g, be settled to 50ml with 70% alcohol, carry out liquid phase after 55 DEG C of water-baths extraction 1h micro-filtrations and measure Monacolin K.Chromatographic column: ZORBAXSB-C18,150mm × 4.6mm × 5 μ m; Moving phase: acetonitrile: water=55:45 (v/v), with phosphoric acid tune pH to 2.5; Column temperature: 28 DEG C; Flow velocity: 1.0mLmin
-1; Sample size: 20 μ L; Detector: UV-detector, detects wavelength 238nm.
Embodiment 1 liquid state fermentation example
Liquid state fermentation substratum is according to glucose 20gL
-1, Soy hydrolysate 50mLL
-1, NaNO
32gL
-1, MgSO
47H
2o1gL
-1, K
2hPO
43H
2o1gL
-1, corn steep liquor 10mLL
-1, pH prepares naturally, 121 DEG C of sterilizing 20min.The Monascus anka Nakazawa et sato CGMCC3.4577 seed liquor of access 15% (V/V), rotating speed 180r/min after mixing, cultivates 2 days for 32 DEG C, cultivates 18 days for 28 DEG C.After fermentation ends, get fermented liquid 55 DEG C of water-bath lixiviate 1h of alcohol of 70%, after micro-filtration, measure Monacolin K content with HPLC, Monacolin K content is 428mg/L.
Embodiment 2 conventional solid-state fermentations
Taking rice as solid state substrate carrier, rice and nutritive medium in mass ratio 1:1 mix, 121 DEG C of sterilizing 20min, the Monascus anka Nakazawa et sato CGMCC3.4577 seed liquor of access 15% (W/W), mix latter 30 DEG C and cultivate 2 days, cultivate 20 days, within every 3 days, turn once therebetween for 28 DEG C.Nutrient composition is glucose 30g/L, Soy hydrolysate 50mL/L, glycerine 400g/L, NaNO
310g/L, K
2hPO
44g/L, MgSO
42g/L, ZnSO
47H
2o8g/L, corn steep liquor 50mL/L.After fermentation ends, high-temperature sample moist heat sterilization post-drying is pulverized, the 55 DEG C of water-bath lixiviate 1h of alcohol with 70%, after micro-filtration, with HPLCMonacolin K content, Monacolin K content is 4380mg/kg, leavened prod yield 53%.
Embodiment 3 solid-liquid combined ferments
Using rice as solid state substrate carrier, it is stand-by that rice is processed 5min through 160 DEG C of Infrared Heating.Liquid state fermentation substratum is good according to formulated, through 121 DEG C of sterilizing 15min, according to the inoculum size access CGMCC3.4577 seed liquor of 10% (V/V), after mixing, rotating speed 180r/min, 32 DEG C cultivate 2 days, cultivate 12 days for 28 DEG C, be transferred to rice matrix according to rice matrix and liquid state fermentation liquid mass ratio 1:1 (W/W) and get on, cultivate 2 days for 30 DEG C, cultivate 12 days for 25 DEG C, after fermentation ends, after high-temperature sterilization, oven dry, pulverize.Be 2165mg/kg with measuring Monacolin K content after 55 DEG C of water-bath extraction 1h micro-filtrations of 70% alcohol, leavened prod yield 76%.
Embodiment 4 solid-liquid combined ferments
Using rice as solid state substrate carrier, millet is through 2000W/m
2it is stand-by that intensity of sunshine (shakeout thickness and be no more than 2cm) is processed 3h.Liquid state fermentation substratum is good according to formulated, through 121 DEG C of sterilizing 15min, according to the inoculum size access CGMCC3.4577 seed liquor of 10% (V/V), rotating speed 180r/min after mixing, cultivates 2 days for 32 DEG C, cultivates 12 for 28 DEG C, it is transferred to rice matrix according to rice matrix and liquid state fermentation liquid mass ratio 1:1 (W/W) and gets on, cultivate 2 days for 30 DEG C, cultivate 12 days for 25 DEG C, after fermentation ends, after high-temperature sterilization, oven dry, pulverize.Be 1863mg/kg with measuring Monacolin K content after 55 DEG C of water-bath extraction 1h micro-filtrations of 70% alcohol, leavened prod yield 78%.
Embodiment 5 solid-liquid combined ferments
Using rice as solid state substrate carrier, it is stand-by that wheat cures processing 5min through 180 DEG C of high temperature.Liquid state fermentation substratum is good according to formulated, through 121 DEG C of sterilizing 15min, according to the inoculum size access CGMCC3.4577 seed liquor of 10% (V/V), rotating speed 180r/min after mixing, cultivates 2 days for 32 DEG C, cultivates 12 days for 28 DEG C, being transferred to rice matrix according to rice matrix and liquid state fermentation liquid mass ratio 1:1 (W/W) gets on, cultivate 2 days for 30 DEG C, cultivate 12 days for 25 DEG C, after fermentation ends, after high-temperature sterilization, oven dry, pulverize.Be 2510mg/kg with measuring Monacolin K content after 55 DEG C of water-bath extraction 1h micro-filtrations of 70% alcohol, leavened prod yield 75%.
Embodiment 6 solid-liquid combined ferments
Using rice as solid state substrate carrier, rice pellets, through the quick-fried 1.5MPa of high pressure vapour, maintains 3-10min air pressure and die-offs, and dries to water content 3% stand-by.Liquid state fermentation substratum is good according to formulated, through 121 DEG C of sterilizing 15min, according to the inoculum size access CGMCC3.4577 seed liquor of 10% (V/V), rotating speed 180r/min after mixing, cultivate 2 days for 32 DEG C, cultivate 12 days for 28 DEG C, being transferred to rice matrix according to solid state substrate and liquid state fermentation liquid mass ratio 1:1 (W/W) gets on, cultivate 2 days for 30 DEG C, cultivate 12 days for 25 DEG C, after fermentation ends, after high-temperature sterilization, oven dry, pulverizing, is 3540mg/kg with measuring Monacolin K content after 55 DEG C of water-baths extraction 1h micro-filtrations of 70% alcohol, leavened prod yield 77%.
Embodiment 7 solid-liquid combined ferments
Using rice as solid state substrate carrier, rice pellets, through the quick-fried 1.5MPa of high pressure vapour, maintains 3-10min air pressure and die-offs, and dries to water content 3% stand-by.Liquid state fermentation substratum is good according to formulated, through 121 DEG C of sterilizing 15min, according to the inoculum size access CGMCC3.4577 seed liquor of 10% (V/V), rotating speed 180r/min after mixing, cultivate 2 days for 32 DEG C, cultivate 12 days for 28 DEG C, being transferred to rice matrix according to solid state substrate and liquid state fermentation liquid mass ratio 1:0.8 (W/W) gets on, cultivate 2 days for 30 DEG C, cultivate 12 days for 25 DEG C, after fermentation ends, after high-temperature sterilization, oven dry, pulverizing, is 2478mg/kg with measuring Monacolin K content after 55 DEG C of water-baths extraction 1h micro-filtrations of 70% alcohol, leavened prod yield 76%.
Embodiment 8 solid-liquid combined ferments
Using rice as solid state substrate carrier, rice pellets, through the quick-fried 1.5MPa of high pressure vapour, maintains 3-10min air pressure and die-offs, and dries to water content 3% stand-by.Liquid state fermentation substratum is good according to formulated, through 121 DEG C of sterilizing 15min, according to the inoculum size access CGMCC3.4577 seed liquor of 10% (V/V), rotating speed 180r/min after mixing, cultivate 2 days for 32 DEG C, cultivate 12 days for 28 DEG C, being transferred to rice matrix according to solid state substrate and liquid state fermentation liquid mass ratio 1:1.3 (W/W) gets on, cultivate 2 days for 30 DEG C, cultivate 12 days for 25 DEG C, after fermentation ends, after high-temperature sterilization, oven dry, pulverizing, is 4603mg/kg with measuring Monacolin K content after 55 DEG C of water-baths extraction 1h micro-filtrations of 70% alcohol, leavened prod yield 80%.
Embodiment 9 solid-liquid combined ferments
Using rice as solid state substrate carrier, rice pellets, through the quick-fried 1.5MPa of high pressure vapour, maintains 3-10min air pressure and die-offs, and dries to water content 3% stand-by.Liquid state fermentation substratum is good according to formulated, through 121 DEG C of sterilizing 15min, according to the inoculum size access CGMCC3.4577 seed liquor of 10% (V/V), rotating speed 180r/min after mixing, cultivate 2 days for 32 DEG C, cultivate 8 days for 28 DEG C, being transferred to rice matrix according to solid state substrate and liquid state fermentation liquid mass ratio 1:1.3 (W/W) gets on, cultivate 2 days for 30 DEG C, cultivate 12 days for 25 DEG C, after fermentation ends, after high-temperature sterilization, oven dry, pulverizing, is 3267mg/kg with measuring Monacolin K content after 55 DEG C of water-baths extraction 1h micro-filtrations of 70% alcohol, leavened prod yield 76%.
Embodiment 10 solid-liquid combined ferments
Using rice as solid state substrate carrier, rice pellets, through the quick-fried 1.5MPa of high pressure vapour, maintains 3-10min air pressure and die-offs, and dries to water content 3% stand-by.Liquid state fermentation substratum is good according to formulated, through 121 DEG C of sterilizing 15min, according to the inoculum size access CGMCC3.4577 seed liquor of 10% (V/V), rotating speed 180r/min after mixing, cultivate 2 days for 32 DEG C, cultivate 16 days for 28 DEG C, being transferred to rice matrix according to solid state substrate and liquid state fermentation liquid mass ratio 1:1.3 gets on, cultivate 2 days for 30 DEG C, cultivate 12 days for 25 DEG C, after fermentation ends, after high-temperature sterilization, oven dry, pulverizing, is 4818mg/kg with measuring monacolin K content after 55 DEG C of water-baths extraction 1h micro-filtrations of 70% alcohol, leavened prod yield 79%.
Embodiment 11 solid-liquid combined ferments
Using rice as solid state substrate carrier, rice pellets, through the quick-fried 1.5MPa of high pressure vapour, maintains 3-10min air pressure and die-offs, and dries to water content 3% stand-by.Liquid state fermentation substratum is good according to formulated, through 121 DEG C of sterilizing 15min, according to the inoculum size access CGMCC3.4577 seed liquor of 10% (V/V), rotating speed 180r/min after mixing, cultivate 2 days for 32 DEG C, cultivate 16 days for 28 DEG C, being transferred to rice matrix according to solid state substrate and liquid state fermentation liquid mass ratio 1:1.3 (W/W) gets on, cultivate 2 days for 30 DEG C, cultivate 8 days for 25 DEG C, after fermentation ends, after high-temperature sterilization, oven dry, pulverizing, is 4181mg/kg with measuring Monacolin K content after 55 DEG C of water-baths extraction 1h micro-filtrations of 70% alcohol, leavened prod yield 83%.
Embodiment 12 solid-liquid combined ferments
Using rice as solid state substrate carrier, rice pellets, through the quick-fried 1.5MPa of high pressure vapour, maintains 3-10min air pressure and die-offs, and dries to water content 3% stand-by.Liquid state fermentation substratum is good according to formulated, through 121 DEG C of sterilizing 15min, according to the inoculum size access CGMCC3.4577 seed liquor of 10% (V/V), rotating speed 180r/min after mixing, cultivate 2 days for 32 DEG C, 28 DEG C of cultivations are transferred to rice matrix according to solid state substrate and liquid state fermentation liquid mass ratio 1:1.3 (W/W) in 16 days and get on, cultivate 2 days for 30 DEG C, cultivate 16 days for 25 DEG C, after fermentation ends, after high-temperature sterilization, oven dry, pulverize, be 4887mg/kg with measuring Monacolin K content after 55 DEG C of water-bath extraction 1h micro-filtrations of 70% alcohol, leavened prod yield 74%.
Although the present invention with preferred embodiment openly as above; but it is not in order to limit the present invention, any person skilled in the art, without departing from the spirit and scope of the present invention; all can do various changes and modification, therefore protection scope of the present invention should be with being as the criterion that claims were defined.
Claims (10)
1. mushy stage combined ferment produces a method for Monacolin K functional Monascus, it is characterized in that, mainly comprises the following steps:
(1) solid feed is carried out to dry heating method pre-treatment, reach not only sterilizing but also make material moisture be down to the object of 0.5%-5%;
(2) cultivate and obtain Monascus anka Nakazawa et sato seed liquor;
(3) Monascus anka Nakazawa et sato liquid state fermentation in early stage;
(4) fermented liquid is accessed in solid feed, proceed later stage solid State Fermentation of Monascus.
2. method according to claim 1, is characterized in that, described solid feed is one or more in barley, wheat, rice, millet, corn or Chinese sorghum.
3. method according to claim 1, it is characterized in that, it is that cereal materials is cured or the quick-fried processing of high pressure vapour through sunlight exposure, Infrared Heating, microwave treatment, high temperature that described step (1) is carried out dry heating method pre-treatment to solid feed, makes material moisture be down to 0.5%-5% and obtains solid-state fermentation substrate.
4. according to the arbitrary described method of claim 1-3, it is characterized in that, described sunlight exposure treatment condition are 1500-2000W/m
2intensity of sunshine is processed 1-4h; Described Infrared Heating is that 0.5-10min is processed in 100-200 DEG C of infrared wavelength 75～1000 μ m heating; Described microwave treatment is that 800-1500W processes 0.5-5min; It is 100-200 DEG C of processing 0.5-2h that described high temperature cures; The quick-fried 1.0-1.8MPa of being of described high pressure vapour maintains 3-10min air pressure and die-offs, and dries to water content 0.5%-10%.
5. method according to claim 4, is characterized in that, the deposit number of described red colouring agent for food, also used as a Chinese medicine bacterial classification is CGMCC3.4577.
6. method according to claim 4, is characterized in that, described step (2) Monascus anka Nakazawa et sato seed liquor is by after activated red colouring agent for food, also used as a Chinese medicine bacterial classification cultivation maturation, prepares spore suspension, and the concentration of spore suspension miospore is not less than 10
7individual/ml; By in spore suspension 10% ratio access by volume seed culture medium, 30 DEG C of 180r/min aerlbic cultures obtain seed liquor for 2 days.
7. method according to claim 6, is characterized in that, described seed culture medium contains glucose 60g/L, peptone 25g/L, corn steep liquor 10mL/L, NaNO
32g/L, K
2hPO
43H
2o1g/L, MgSO
40.5g/L.
8. method according to claim 4, it is characterized in that, when described step (3) Monascus anka Nakazawa et sato liquid state fermentation in early stage, in 10-20% ratio, cultured Monascus anka Nakazawa et sato seed liquor is accessed in the liquid state fermentation substratum after sterilizing, 32 DEG C, 180r/min are cultivated 2 days, and then 28 DEG C, 180r/min are cultivated 8-18 days.
9. method according to claim 8, is characterized in that, described liquid state fermentation substratum contains glucose 20gL
-1, Soy hydrolysate 50mLL
-1, NaNO
32gL
-1, MgSO
47H
2o1gL
-1, K
2hPO
43H
2o1gL
-1, corn steep liquor 10mLL
-1, pH nature.
10. method according to claim 4, it is characterized in that, described step (4) solid state fermentation is according to solid-state fermentation substrate and liquid state fermentation liquid mass ratio 1:(0.5-2 by liquid state fermentation liquid) ratio be transferred to solid-state fermentation substrate, 25-32 DEG C ferments 2 days, then 20-30 DEG C of fermentation 6-20 days, every 48h stirs once.
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| CN104560790A (en) * | 2014-12-15 | 2015-04-29 | 北京农学院 | Functional lactobacillus plantarum and preparation method of compound bacterial powder of functional lactobacillus plantarum |
| CN104651410A (en) * | 2015-02-27 | 2015-05-27 | 福建师范大学 | Method for preparing red yeast rice with high color value |
| CN104745399A (en) * | 2015-04-20 | 2015-07-01 | 江南大学 | Brewing method capable of increasing content of Monacolin K in monascus rice wine |
| CN111218480A (en) * | 2020-02-25 | 2020-06-02 | 扬州市职业大学(扬州市广播电视大学) | Preparation method of solid-state fermentation red yeast rice with high yield of monacolin K |
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| CN104560790A (en) * | 2014-12-15 | 2015-04-29 | 北京农学院 | Functional lactobacillus plantarum and preparation method of compound bacterial powder of functional lactobacillus plantarum |
| CN104651410A (en) * | 2015-02-27 | 2015-05-27 | 福建师范大学 | Method for preparing red yeast rice with high color value |
| CN104651410B (en) * | 2015-02-27 | 2018-04-13 | 福建师范大学 | A kind of preparation method of High color values red yeast rice |
| CN104745399A (en) * | 2015-04-20 | 2015-07-01 | 江南大学 | Brewing method capable of increasing content of Monacolin K in monascus rice wine |
| CN111218480A (en) * | 2020-02-25 | 2020-06-02 | 扬州市职业大学(扬州市广播电视大学) | Preparation method of solid-state fermentation red yeast rice with high yield of monacolin K |
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