CN104109624B - A kind of preparation method of bitter buckwheat rich selenium reed bamboo shoot vinegar that germinates - Google Patents
A kind of preparation method of bitter buckwheat rich selenium reed bamboo shoot vinegar that germinates Download PDFInfo
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- CN104109624B CN104109624B CN201410342084.7A CN201410342084A CN104109624B CN 104109624 B CN104109624 B CN 104109624B CN 201410342084 A CN201410342084 A CN 201410342084A CN 104109624 B CN104109624 B CN 104109624B
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Abstract
The invention provides a kind of preparation method of the bitter buckwheat reed bamboo shoot vinegar that germinates, belong to vinegar brewing field.Adopt germinate bitter buckwheat and the mixed fermentation of reed bamboo shoot, in fermenting process, add amylase, saccharifying enzyme, polygalacturonase, cellulase, yeast saccharomyces cerevisiae, acetic bacteria.Aminoacids content 3.8-4.1%, rutin content 0.084-0.118%, alpha-aminobutyric acid content 8-12.5mg/100g, selenium content 185-250ug/L in obtained vinegar.Therefore, the sprout brown rice asparagus fermented vinegar nutritive ingredient using the method to produce is many, and local flavor is mellow, and mouthfeel acid is refreshing, is the vinegar based article simultaneously with desirable mouth feel and nutritional health function.
Description
technical field:
The invention belongs to vinegar brewing field, more particularly, the present invention relates to a kind of preparation method of bitter buckwheat rich selenium reed bamboo shoot vinegar that germinates.
background technology:
Reed bamboo shoot are that a kind of self-sow is in the wild plant on beach, continent, the middle and lower reach of Yangtze River.Reed bamboo shoot are the tender stem of reed reed, are commonly called as bavin bamboo shoot or asparagus.Reed bamboo shoot are a kind of pure wild natural phant, its production environment is not subject to the pollution of modern industry and mankind's activity, process of growth does not use agricultural chemicals, chemical fertilizer and plant-growth regulator, and be rich in the nutritive ingredients such as the necessary multiple amino acids of human body, trace element and Mierocrystalline cellulose, there is the health-care effecies such as eliminating is greasy, intestines are led in clearing stomach, weight reducing beauty treatment.Its delicate mouthfeel, delicious meat, boil not rotten, tender and crisp good to eat, unique flavor for a long time, enjoy the favor of people, become entertain the guests at a banquet, the good merchantable brand of presenting friends.
Bitter buckwheat and Radix Et Rhizoma Fagopyri Tatarici, formal name used at school
tartarbuckwheat is the king of five cereals.It is the medicine-food two-purpose crop that occurring in nature is very few, according to "
compendium of Materia Medica" record: bitter buckwheat bitter, property is flat cold, the real stomach of energy, beneficial strength, continuous spirit, sharp knowledge, and refining the five internal organs slag is dirty.Bitter buckwheat integrates seven major nutrient, have remarkable healthy nutritive value and outstanding dietotherapy effect, can calm the nerves, wind pain is swollen in the wide intestines of lively atmosphere blood, sending down abnormally ascending, heat-clearing, long-pendingization of dispelling is stagnant, gut purge, ease constipation, defaecation, cough-relieving, relieving asthma, anti-inflammatory, antianaphylaxis, cardiac stimulant, fat-reducing, the effect such as beauty treatment.Especially the main component rutin (also known as vitamin P) in Tartary buckwheat biological flavonoid, has vessel softening, improves microcirculation, clearing heat and detoxicating, promoting blood circulation and removing blood stasis, toxin expelling myogenic, hypoglycemic, glucose in urine, blood fat, beneficial air lift god, strengthen Regular Insulin peripheral action, so also claim " three fall food ".
Selenium is the trace element of needed by human.Selenium participates in multiple selenoenzyme and Selenonic protein in synthesized human.Wherein
selenoperoxidase, in vivo catalysis hydroperoxide or
lipid peroxidechange water or various alcohols into, eliminate the biomembranous attack of radical pair, protection microbial film is from oxidative damage.
Now, existing correlative study person develops buckwheat vinegar, such as: Chinese Patent Application No. CN20120046603.6 " buckwheat vinegar and production method thereof " etc.What study is all the vinegar utilized without the bitter buckwheat germinateed and other material mixed fermentations multiple, and material composition is complicated, and mouthfeel and nutrient composition content are difficult to control.Find by retrieving domestic and international prior art, at present also not with the report that germinate bitter buckwheat and reed bamboo shoot are major ingredient fermented vinegar.
Summary of the invention
The object of the invention is the production technique providing a kind of bitter buckwheat rich selenium reed bamboo shoot vinegar that germinates.The present invention adopts germinate bitter buckwheat and the mixed fermentation of reed bamboo shoot, what take full advantage of raw material during the fermentation enriches enzyme system, the composition of health-care effect is fully incorporated in vinegar, both mouthfeel and the local flavor of vinegar had been improved, enhance again the health-care effect of the bitter buckwheat reed bamboo shoot vinegar that germinates, the health-care effect of vinegar and quality are significantly promoted.
A preparation method for bitter buckwheat richness of germinateing selenium reed bamboo shoot vinegar, is characterized in that it is prepared according to the following steps:
(1) select bitter buckwheat, bitter buckwheat is put into the sodium selenite solution of 25mg/L, the thermostat water bath being placed in 30 DEG C of temperature soaks 12h.Then the growth hormone that lotus seeds put into 450mg/L is soaked 18-20h, carry out cultivation and germinate under 30-35 DEG C of constant temperature culture condition, the obtained bitter buckwheat that germinates, by the bitter buckwheat colloidal mill defibrination that germinates, cooled and filtered, obtains selenium-rich germinated bitter buckwheat juice.
(2) get selenium-rich germinated bitter buckwheat juice that fresh, cleaned reed bamboo shoot and step (1) obtain in mass ratio 1:1 mix, use colloidal mill defibrination, 2-4 is added doubly to the water of dry material in slurries, by mixing the amylase 0.40-0.50g/kg adding enzyme 10000U/g alive with the weight ratio (g/Kg) of raw material, the saccharifying enzyme 0.30-0.60g/kg of enzyme 8000U/g alive, the polygalacturonase 0.15-0.20g/kg of enzyme 12000U/g alive, the cellulase 0.10-0.15g/kg of enzyme 6000U/g alive, enzymolysis 15 hours under 40 DEG C of conditions;
(3) in the mixed solution obtained to step (2), by adding yeast saccharomyces cerevisiae 0.40-0.55g/kg with the weight ratio (g/Kg) of raw material, being placed in 33-36 DEG C of condition bottom fermentation and obtaining distiller's wort in 8 days;
(4) fermenting in complete distiller's wort, by adding acetic bacteria 0.50-0.60g/kg with the weight ratio (g/Kg) of raw material, under 30-33 DEG C of condition, adopting solid state fermentation 7 days;
(5) fermented liquid step (4) obtained is centrifugal, filter, sterilizing obtains clear liquor, then carries out diluting, packing, can obtain the bitter buckwheat reed bamboo shoot vinegar that germinates.
Preferably, the preparation method of bitter buckwheat rich selenium reed bamboo shoot vinegar that germinates as above, wherein the yeast saccharomyces cerevisiae described in step (3) is yeast saccharomyces cerevisiae CICC1027.
Preferably, the preparation method of bitter buckwheat rich selenium reed bamboo shoot vinegar that germinates as above, wherein the acetic bacteria described in step (4) is acetic bacteria CICC21870.
The present invention is relative to the existing processing technology of vinegar, and tool has the following advantages:
(1) at present not with the production method that germinate bitter buckwheat and reed bamboo shoot are waste fermentation vinegar, and the bitter buckwheat of germination rich selenium reed bamboo shoot vinegar that the present invention produces, not only there is the excellent flavor of reed bamboo shoot, and due to the distinctive functional property of bitter buckwheat of germinateing, the vinegar that the present invention is produced is more suitable for " three high " edible for patients.
(2) adopt solid fermentating mode, what utilize the bitter buckwheat of germination and the glycolysis of reed bamboo shoot to produce enriches enzyme system, makes raw material glycolysis more
Add fully, product special flavour is mellow, and mouthfeel acid is refreshing.Aminoacids content 3.8-4.1%, rutin content 0.094-0.118%, alpha-aminobutyric acid content 8-12.5mg/100g, selenium content 185-250ug/L in obtained vinegar, ensure that nutritive ingredient abundant in vinegar, enhance its health-care effect.
Embodiment
Be below specific embodiments of the invention, technical scheme of the present invention is done to describing further, but protection scope of the present invention be not limited to these embodiments.Every do not deviate from the present invention's design change or equivalent substituting include within protection scope of the present invention.
embodiment 1
(1) select bitter buckwheat 120kg, bitter buckwheat is put into the sodium selenite solution of 25mg/L, the thermostat water bath being placed in 30 DEG C of temperature soaks 12h.Then the growth hormone that lotus seeds put into 450mg/L is soaked 18h, carry out cultivation and germinate under 30 DEG C of constant temperature culture conditions, the obtained bitter buckwheat that germinates, by the bitter buckwheat colloidal mill defibrination that germinates, cooled and filtered, obtains selenium-rich germinated bitter buckwheat juice.
(2) fresh, cleaned reed bamboo shoot 120kg is got, colloidal mill defibrination is used after the bitter buckwheat of the germination obtained with step (1) mixes, 480kg water is added in slurries, amylase (enzyme 10000U/g alive) 0.40g/kg is added by mixing with the weight ratio (g/Kg) of raw material, saccharifying enzyme (enzyme 8000U/g alive) 0.30g/kg, polygalacturonase (enzyme 12000U/g alive) 0.15g/kg, cellulase (enzyme 6000U/g alive) 0.10g/kg, enzymolysis 15 hours under 40 DEG C of conditions.
(3) in the mixed solution obtained in step (2), by adding yeast saccharomyces cerevisiae (CICC1027) 0.40g/kg with the weight ratio (g/Kg) of raw material, being placed in 33 DEG C of condition bottom fermentations and obtaining distiller's wort in 8 days.
(4) in the distiller's wort that fermentation is complete, by adding acetic bacteria (CICC21870) 0.50g/kg with the weight ratio (g/Kg) of raw material, solid state fermentation 7 days under 30 DEG C of conditions, are adopted.
(5) centrifugal for the fermented liquid of acquisition, filtration, sterilizing are obtained clear liquor, then carry out diluting, packing, the bitter buckwheat reed bamboo shoot vinegar that germinates can be obtained.
embodiment 2
(1) select bitter buckwheat 150kg, bitter buckwheat is put into the sodium selenite solution of 25mg/L, the thermostat water bath being placed in 30 DEG C of temperature soaks 12h.Then the growth hormone that lotus seeds put into 450mg/L is soaked 20h, carry out cultivation and germinate under 35 DEG C of constant temperature culture conditions, the obtained bitter buckwheat that germinates, by the bitter buckwheat colloidal mill defibrination that germinates, cooled and filtered, obtains selenium-rich germinated bitter buckwheat juice.
(2) fresh, cleaned reed bamboo shoot 150kg is got, colloidal mill defibrination is used with after the bitter buckwheat mixing of germination that step (1) obtains, 1200kg water is added in slurries, amylase (enzyme 10000U/g alive) 0.50g/kg is added by mixing with the weight ratio (g/Kg) of raw material, saccharifying enzyme (enzyme 8000U/g alive) 0.60g/kg, polygalacturonase (enzyme 12000U/g alive) 0.20g/kg, cellulase (enzyme 6000U/g alive) 0.15g/kg, enzymolysis 15 hours under 40 DEG C of conditions.
(3) in the mixed solution obtained to step (2), by adding yeast saccharomyces cerevisiae (CICC1027) 0.55g/kg with the weight ratio (g/Kg) of raw material, being placed in 36 DEG C of condition bottom fermentations and obtaining distiller's wort in 8 days.
(4) in the distiller's wort that fermentation is complete, by adding acetic bacteria (CICC21870) 0.60g/kg with the weight ratio (g/Kg) of raw material, solid state fermentation 7 days under 33 DEG C of conditions, are adopted.
(5) by centrifugal for the fermented liquid obtained, filter, sterilizing obtains clear liquor, then carries out diluting, packing, and can obtain the bitter buckwheat reed bamboo shoot vinegar that germinates.
comparative example 1
The supplementary material kind that this embodiment uses with embodiment 1, quantity of adding, order of addition and the fermentation condition in each stage are all identical, only in comparative example, do not carry out germination treatment to bitter buckwheat.
comparative example 2
Do not use various corresponding zymin in this embodiment, namely lack the amylase in example 2, saccharifying enzyme, polygalacturonase, cellulase, all the other steps are all identical with example 2.
comparative example 3
This embodiment, compared with embodiment 1, does not use raw material reed bamboo shoot, and all the other steps are all identical with embodiment 1.
comparative example 4
This embodiment, compared with embodiment 2, does not carry out rich selenium, and all the other steps are all identical with embodiment 2.
Germination prepared by embodiment of the present invention 1-2 and comparative example 1-4 bitter buckwheat reed bamboo shoot vinegar, its attributional analysis is as following table:
Table 1 embodiment attributional analysis table
。
Claims (3)
1. a preparation method for bitter buckwheat richness of germinateing selenium reed bamboo shoot vinegar, is characterized in that: the method comprises the following steps:
(1) select bitter buckwheat, bitter buckwheat is put into the sodium selenite solution of 25mg/L, the thermostat water bath being placed in 30 DEG C of temperature soaks 12h; Then the growth hormone that bitter buckwheat puts into 450mg/L is soaked 18-20h, carry out cultivation and germinate under 30-35 DEG C of constant temperature culture condition, the obtained bitter buckwheat that germinates, by the bitter buckwheat colloidal mill defibrination that germinates, cooled and filtered, obtains selenium-rich germinated bitter buckwheat juice;
(2) fresh, cleaned reed bamboo shoot are chosen, with the bitter buckwheat juice of the germination of step (1) gained in mass ratio 1:1 mix, use colloidal mill defibrination, 2-4 is added doubly to the water of dry material in slurries, by mixing the amylase 0.40-0.50g/kg adding enzyme 10000U/g alive with the weight ratio of raw material, the saccharifying enzyme 0.30-0.60g/kg of enzyme 8000U/g alive, the polygalacturonase 0.15-0.20g/kg of enzyme 12000U/g alive, the cellulase 0.10-0.15g/kg of enzyme 6000U/g alive, enzymolysis 15 hours under 40 DEG C of conditions;
(3) in the mixed solution obtained in step (2), add yeast saccharomyces cerevisiae 0.40-0.55g/kg by the weight ratio with raw material, be placed in 33-36 DEG C of condition bottom fermentation and obtain distiller's wort in 8 days;
(4) in the distiller's wort that fermentation is complete, add acetic bacteria 0.50-0.60g/kg by the weight ratio with raw material, under 30-33 DEG C of condition, adopt solid state fermentation 7 days;
(5) fermented liquid step (4) obtained is centrifugal, filter, sterilizing obtains clear liquor, then carries out diluting, packing, can obtain the bitter buckwheat reed bamboo shoot vinegar that germinates.
2., according to the preparation method of the bitter buckwheat of germination according to claim 1 rich selenium reed bamboo shoot vinegar, it is characterized in that: the yeast saccharomyces cerevisiae described in step (3) is yeast saccharomyces cerevisiae CICC1027.
3., according to the preparation method of the bitter buckwheat of germination according to claim 1 rich selenium reed bamboo shoot vinegar, it is characterized in that: the acetic bacteria described in step (4) is acetic bacteria CICC21870.
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| JP2013005779A (en) * | 2011-06-27 | 2013-01-10 | Q P Corp | Vegetable vinegar and processed food using the same |
| CN103087895A (en) * | 2013-01-05 | 2013-05-08 | 水城县满全农业开发有限公司 | Tartary buckwheat vinegar submerged fermentation process |
| CN103351997A (en) * | 2013-07-14 | 2013-10-16 | 山西银杏食品科技有限公司 | Selenium-enriched vinegar preparation method |
| CN103734836B (en) * | 2014-01-28 | 2015-01-07 | 程健博 | Method for preparing germinated black tartary buckwheat and hawthorn fruit vinegar beverage |
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Effective date of registration: 20201119 Address after: No.8, yangqueba group, yangqueba village, wuyangba sub district office, Enshi Tujia and Miao Autonomous Prefecture, Hubei Province Patentee after: ENSHI XISHENG PLANT TECHNOLOGY Co.,Ltd. Address before: 430068 Wuchang City, Wuhan Province, South Lake, Li Jia Dun village, No. 1, No., No. 1 Patentee before: HUBEI University OF TECHNOLOGY |