CN104099236A - 一种基于微管液路板的精子优选玻片及优选方法 - Google Patents
一种基于微管液路板的精子优选玻片及优选方法 Download PDFInfo
- Publication number
- CN104099236A CN104099236A CN201410305044.5A CN201410305044A CN104099236A CN 104099236 A CN104099236 A CN 104099236A CN 201410305044 A CN201410305044 A CN 201410305044A CN 104099236 A CN104099236 A CN 104099236A
- Authority
- CN
- China
- Prior art keywords
- sperm
- concentration
- collecting tank
- microtubule
- tank
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000029749 Microtubule Human genes 0.000 title claims abstract description 18
- 108091022875 Microtubule Proteins 0.000 title claims abstract description 18
- 210000004688 microtubule Anatomy 0.000 title claims abstract description 18
- 239000007788 liquid Substances 0.000 title abstract description 5
- 238000010187 selection method Methods 0.000 title 1
- 235000015097 nutrients Nutrition 0.000 claims abstract description 14
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims description 8
- 229920002674 hyaluronan Polymers 0.000 claims description 8
- 229960003160 hyaluronic acid Drugs 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 6
- 239000011734 sodium Substances 0.000 claims description 6
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 claims description 4
- 229960003531 phenolsulfonphthalein Drugs 0.000 claims description 4
- 210000000582 semen Anatomy 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 3
- 229910019142 PO4 Inorganic materials 0.000 claims description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 3
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 3
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 3
- LLSDKQJKOVVTOJ-UHFFFAOYSA-L calcium chloride dihydrate Chemical compound O.O.[Cl-].[Cl-].[Ca+2] LLSDKQJKOVVTOJ-UHFFFAOYSA-L 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims description 3
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 claims description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 3
- 239000010452 phosphate Substances 0.000 claims description 3
- 239000011591 potassium Substances 0.000 claims description 3
- 229910052700 potassium Inorganic materials 0.000 claims description 3
- 229910052708 sodium Inorganic materials 0.000 claims description 3
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 3
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 3
- NGSFWBMYFKHRBD-UHFFFAOYSA-N sodium;2-hydroxypropanoic acid Chemical compound [Na+].CC(O)C(O)=O NGSFWBMYFKHRBD-UHFFFAOYSA-N 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 6
- 239000002699 waste material Substances 0.000 abstract 4
- 238000012216 screening Methods 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 7
- 238000011084 recovery Methods 0.000 description 5
- 238000013016 damping Methods 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 238000011144 upstream manufacturing Methods 0.000 description 4
- 210000003756 cervix mucus Anatomy 0.000 description 3
- 238000000432 density-gradient centrifugation Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 230000004899 motility Effects 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000009328 Perro Species 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000000469 anti-sperm effect Effects 0.000 description 1
- 230000010165 autogamy Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 239000002520 smart material Substances 0.000 description 1
- 230000019100 sperm motility Effects 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0608—Germ cells
- C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/70—Polysaccharides
- C12N2533/80—Hyaluronan
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Molecular Biology (AREA)
- Sustainable Development (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
本发明公开了一种基于微管液路板的精子优选玻片，包括样品池和精子收集池，在样品池与精子收集池之间设置有1层微管通道，精子优选玻片上还设置有1～3个废弃池，在废弃池与精子收集池之间设置有1层微管通道，微管通道的内径为10～200微米，微管通道距样品池、精子收集池和废弃池的池底50～100微米。利用精子培养液对精子的主动吸引和在微管液路板流动，精子随精子培养液主动流动到精子收集池中，利用精子收集池的透明质液的黏附作用，粘住活力高的精子，质量差的精子则继续随精子培养液流动到达废弃池中，精子收集池中基本都是高活力的精子。
Description
技术领域
本发明涉及精子优选，具体涉及一种基于微管液路板的精子优选玻片及优选方法。
背景技术
精子活力是受孕的关键因素之一，精子活率高的精液，其活动力强的精子数量相对较高，因此受孕的机率也高。目前广泛采用的精子优选技术有上游技术、密度梯度离心技术等优选技术。上游技术是利用精子自身活力向上泳动的原理而设计，精子在上游过程中，可以脱离精浆中某些内源性的抑精物质，如抗精子抗体，各类细胞等，并使精子在体外活动时间得以延长，但上游技术对高质量精子回收率相对较低。密度梯度离心技术处理后高质量精子回收率提高，但易造成精子DNA过氧化损伤，精子质量下降。中国专利公告号为CN102352305的发明专利，则公开了利用微流控芯技术，检测和筛选精子，其原理是利用宫颈粘液在样品池中吸附活力稍差的精子及白细胞、上皮细胞等，再利用缓冲液加样池中加入缓冲液，通过缓冲液液流将样品池中的活力高精子带到样品收集池中，达到高活力精子的筛选和收集，但该发明专利技术较复杂，既要宫颈粘液吸附，又要缓冲液被动将精子流冲到样品收集池，精子不是主动流动，精子质量好坏不易控制。
发明内容
本发明所要解决的技术问题是提供一种筛选简单，筛选效果好，回收率较高，精子质量易控制的基于微管液路板的精子优选玻片。本发明也提供了利用该精子优选玻片优选精子的方法。
本发明解决上述技术问题所采用的技术方案为：
一种基于微管液路板的精子优选玻片，包括样品池和精子收集池，在所述样品池与所述精子收集池之间设置有1层微管通道，所述精子优选玻片上还设置有1～3个废弃池，在所述废弃池与所述精子收集池之间设置有1层微管通道，所述微管通道的内径为10～200微米，所述微管通道距所述样品池、所述精子收集池和所述废弃池的池底50～100微米。微管通道可以是直管，也可以是曲管。
一种利用基于微管液路板的精子优选玻片优选精子的方法，其步骤如下：
a、先在精子收集池加入透明质酸（可以市售，也可以自配），所述透明质酸表面距所述精子收集池的池底50～100微米，再将精液移入样品池中，同时在样品池中加满精子培养液，所述精子培养液中含有浓度为0.1mol/L的氯化钠、浓度为0.4mmol/L的磷酸二氢钾、浓度为2.7mmol/L 的葡萄糖、浓度为4.7mmol/L的氯化钾、浓度为0.2mmol/L 的七水硫酸镁、浓度为2mmol/L 的二水氯化钙、浓度为0.02mol/L的 N-2-羟乙基哌嗪-N-2-乙磺酸二钠盐（Hepes-Na）、浓度为4mmol/L 的碳酸氢钠、浓度为0.3mmol/L丙酮酸钠、体积百分浓度为0.34%的乳酸钠（C3H5O3Na）、质量百分浓度为0.002%的酚红（Phenol Red）；
b、将精子优选玻片置于37℃下，恒温15～45分钟，在所述精子收集池中得到优选的精子。
与现有技术相比，本发明的优点在于一种基于微管液路板的精子优选玻片，包括样品池和精子收集池，在样品池与精子收集池之间设置有1层微管通道，精子优选玻片上还设置有1～3个废弃池，在废弃池与精子收集池之间设置有1层微管通道，微管通道的内径为10～200微米，微管通道距样品池、精子收集池和废弃池的池底50～100微米。利用精子培养液对精子的主动吸引和在微管液路板流动，精子随精子培养液主动流动到精子收集池中，利用精子收集池的透明质酸对高活力精子的黏附作用，粘住活力高的精子，质量差的精子则继续随精子培养液流动到达废弃池中，精子收集池中基本都是高活力的精子。本发明的方法筛选简单，精子回收率较高，同时避免精子质量下降，筛选效果好，因此本发明是一种筛选简单，筛选效果好，回收率较高，精子质量易控制的基于微管液路板的精子优选玻片。
附图说明
图1为实施例1结构示意图；
图2为实施例2结构示意图。
具体实施方式
以下结合附图实施例对本发明作进一步详细描述。
实施例1
如图1所示的一种基于微管液路板的精子优选玻片，包括样品池1和精子收集池2，在样品池1与精子收集池2之间设置有1层直管型微管通道3，精子优选玻片上还设置有2个废弃池4，在废弃池4与精子收集池2之间设置有1层直管型微管通道3，微管通道3的内径为10～200微米（如人的精子可以设为35微米），微管通道3距样品池1、精子收集池2和废弃池4的池底50～100微米。
实施例2
与实施例1基本相同，所不同的只是废弃池为1个或3个，微管通道为曲管型。
实施例3
一种利用实施例1或实施例2的精子优选玻片优选精子的方法，先在精子收集池加入透明质酸，透明质酸表面距精子收集池的池底50～100微米，再将精液移入样品池中，同时在样品池中加满精子培养液，精子培养液中含有浓度为0.1mol/L的氯化钠、浓度为0.4mmol/L的磷酸二氢钾、浓度为2.7mmol/L 的葡萄糖、浓度为4.7mmol/L的氯化钾、浓度为0.2mmol/L 的七水硫酸镁、浓度为2mmol/L 的二水氯化钙、浓度为0.02mol/L的 Hepes-Na、浓度为4mmol/L 的碳酸氢钠、浓度为0.3mmol/L丙酮酸钠、体积百分浓度为0.34%的C3H5O3Na、质量百分浓度为0.002%的Phenol Red；将精子优选玻片置于37℃下，恒温15～45分钟，在精子收集池中得到优选的精子。有利于后续人工授精、体外受精、显微受精等操作。本发明的方法与CN102352305相比是反向思路，活力高的精子在精子收集池中沉积，活力低的精子则随精子培养液冲入到废弃池。且透明质酸为常用试剂，比宫颈粘液的采集容易，使用方便。
上述精子来源可以是哺乳动物，如人、小鼠、大鼠、兔、羊、狗、猫、牛等，也可以是其它动物，如鱼类、二栖类等。
Claims (2)
1.一种基于微管液路板的精子优选玻片，包括样品池和精子收集池，在所述样品池与所述精子收集池之间设置有1层微管通道，其特征在于所述精子优选玻片上还设置有1～3个废弃池，在所述废弃池与所述精子收集池之间设置有1层微管通道，所述微管通道的内径为10～200微米，所述微管通道距所述样品池、所述精子收集池和所述废弃池的池底50～100微米。
2.利用权利要求1所述的一种基于微管液路板的精子优选玻片优选精子的方法，其特征在于步骤如下：
a、先在精子收集池加入透明质酸，所述透明质酸表面距所述精子收集池的池底50～100微米，再将精液移入样品池中，同时在样品池中加满精子培养液，所述精子培养液中含有浓度为0.1mol/L的氯化钠、浓度为0.4mmol/L的磷酸二氢钾、浓度为2.7mmol/L 的葡萄糖、浓度为4.7mmol/L的氯化钾、浓度为0.2mmol/L 的七水硫酸镁、浓度为2mmol/L 的二水氯化钙、浓度为0.02mol/L的 N-2-羟乙基哌嗪-N-2-乙磺酸二钠盐、浓度为4mmol/L 的碳酸氢钠、浓度为0.3mmol/L丙酮酸钠、体积百分浓度为0.34%的乳酸钠、质量百分浓度为0.002%的酚红；
b、将精子优选玻片置于37℃下，恒温15～45分钟，在所述精子收集池中得到优选的精子。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410305044.5A CN104099236B (zh) | 2014-06-30 | 2014-06-30 | 一种基于微管液路板的精子优选玻片及优选方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410305044.5A CN104099236B (zh) | 2014-06-30 | 2014-06-30 | 一种基于微管液路板的精子优选玻片及优选方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104099236A true CN104099236A (zh) | 2014-10-15 |
| CN104099236B CN104099236B (zh) | 2016-08-24 |
Family
ID=51667782
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410305044.5A Active CN104099236B (zh) | 2014-06-30 | 2014-06-30 | 一种基于微管液路板的精子优选玻片及优选方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104099236B (zh) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104762192A (zh) * | 2015-03-26 | 2015-07-08 | 浙江星博生物科技有限公司 | 一种人类精子优选和体外受精的仿生装置 |
| CN105154320A (zh) * | 2015-10-12 | 2015-12-16 | 深圳市博锐德生物科技有限公司 | 透明质酸及其盐或衍生物包被的单精子选择装置及应用 |
| WO2022160364A1 (zh) * | 2021-02-01 | 2022-08-04 | 徐小杨 | 一种精子优选装置及其操作方法 |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1745292A (zh) * | 2002-12-09 | 2006-03-08 | 先进流体实验室股份有限公司 | 男性生育能力分析方法和装置 |
| JP2007198765A (ja) * | 2006-01-24 | 2007-08-09 | Toray Ind Inc | 分析チップおよび分析方法 |
| CN101487841A (zh) * | 2009-02-13 | 2009-07-22 | 刘瑜 | 一种包被载体及其在检测精子成熟度和无创分离成熟精子方法中的应用 |
| CN201335836Y (zh) * | 2008-12-29 | 2009-10-28 | 广州市第一人民医院 | 用于分选精子的微流控芯片结构 |
| CN201469382U (zh) * | 2009-07-10 | 2010-05-19 | 曾勇 | 用于体外受精的培养皿 |
| CN202786220U (zh) * | 2012-08-10 | 2013-03-13 | 中国科学院深圳先进技术研究院 | 微流控生物芯片 |
| TW201343916A (zh) * | 2012-02-03 | 2013-11-01 | Neobios Pte Ltd | 被動式微流體裝置及其形成方法 |
| CN103773672A (zh) * | 2014-02-08 | 2014-05-07 | 浙江省医学科学院 | 一种检测人精子活动力和化学趋向性的方法及其装置 |
-
2014
- 2014-06-30 CN CN201410305044.5A patent/CN104099236B/zh active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1745292A (zh) * | 2002-12-09 | 2006-03-08 | 先进流体实验室股份有限公司 | 男性生育能力分析方法和装置 |
| JP2007198765A (ja) * | 2006-01-24 | 2007-08-09 | Toray Ind Inc | 分析チップおよび分析方法 |
| CN201335836Y (zh) * | 2008-12-29 | 2009-10-28 | 广州市第一人民医院 | 用于分选精子的微流控芯片结构 |
| CN101487841A (zh) * | 2009-02-13 | 2009-07-22 | 刘瑜 | 一种包被载体及其在检测精子成熟度和无创分离成熟精子方法中的应用 |
| CN201469382U (zh) * | 2009-07-10 | 2010-05-19 | 曾勇 | 用于体外受精的培养皿 |
| TW201343916A (zh) * | 2012-02-03 | 2013-11-01 | Neobios Pte Ltd | 被動式微流體裝置及其形成方法 |
| CN202786220U (zh) * | 2012-08-10 | 2013-03-13 | 中国科学院深圳先进技术研究院 | 微流控生物芯片 |
| CN103773672A (zh) * | 2014-02-08 | 2014-05-07 | 浙江省医学科学院 | 一种检测人精子活动力和化学趋向性的方法及其装置 |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104762192A (zh) * | 2015-03-26 | 2015-07-08 | 浙江星博生物科技有限公司 | 一种人类精子优选和体外受精的仿生装置 |
| CN105154320A (zh) * | 2015-10-12 | 2015-12-16 | 深圳市博锐德生物科技有限公司 | 透明质酸及其盐或衍生物包被的单精子选择装置及应用 |
| WO2022160364A1 (zh) * | 2021-02-01 | 2022-08-04 | 徐小杨 | 一种精子优选装置及其操作方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104099236B (zh) | 2016-08-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Nagata et al. | Live births from artificial insemination of microfluidic-sorted bovine spermatozoa characterized by trajectories correlated with fertility | |
| Tempest et al. | Endometrial stem cell markers: current concepts and unresolved questions | |
| Almiñana et al. | Extracellular vesicles in the oviduct: progress, challenges and implications for the reproductive success | |
| Morisawa et al. | Effects of osmolality and potassium on motility of spermatozoa from freshwater cyprinid fishes | |
| CN104099293A (zh) | 一种基于微孔薄膜的精子优选方法 | |
| CN102242055B (zh) | 一种精子活力评价及筛选的方法及其专用微流控芯片装置 | |
| CN1441652A (zh) | 微流控通道胚胎和/或卵母细胞处理,分析和生物评价 | |
| Fujiwara et al. | Promoting roles of embryonic signals in embryo implantation and placentation in cooperation with endocrine and immune systems | |
| Asaadi et al. | Extracellular vesicles from follicular and ampullary fluid isolated by density gradient ultracentrifugation improve bovine embryo development and quality | |
| CN101504407A (zh) | 以少量精细胞对哺乳动物进行有性别选择的授精 | |
| CN104099236A (zh) | 一种基于微管液路板的精子优选玻片及优选方法 | |
| Romero-Aguirregomezcorta et al. | Sperm selection by rheotaxis improves sperm quality and early embryo development | |
| CN103421675A (zh) | 精子趋向性评价及筛选的方法及其专用微流控芯片系统 | |
| Khodamoradi et al. | Recent microfluidic innovations for sperm sorting | |
| Neculai-Valeanu et al. | Game-changing approaches in sperm sex-sorting: microfluidics and nanotechnology | |
| CN104099237A (zh) | 一种基于微管通道的精子优选装置及优选方法 | |
| Li et al. | Separation of motile sperm for in vitro fertilization from frozen-thawed bull semen using progesterone induction on a microchip | |
| Dorado et al. | Single-layer centrifugation through PureSperm® 80 selects improved quality spermatozoa from frozen-thawed dog semen | |
| Navarro-Serna et al. | Replacement of albumin by preovulatory oviductal fluid in swim-up sperm preparation method modifies boar sperm parameters and improves in vitro penetration of oocytes | |
| CN206324012U (zh) | 一种大型溞慢性毒性试验自动换水和分离装置 | |
| Jo et al. | Effect of antibodies binding to Y chromosome-bearing sperm conjugated with magnetic nanoparticles on bull sperm characteristics | |
| Gimeno et al. | Effects of in vitro interactions of oviduct epithelial cells with frozen–thawed stallion spermatozoa on their motility, viability and capacitation status | |
| Pinto-Pinho et al. | The history and prospects of rabbit sperm sexing | |
| Xu et al. | Vitrification of pronuclear zygotes Perturbs porcine zygotic genome activation | |
| Singh et al. | Sexed semen technology in cattle: A revolutionary technique in Indian dairy industry |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 315040 Zhejiang high tech District of Ningbo City Xinhui Road No. 139 Building No. 2 Patentee after: Chen Binglai Address before: 315040 Zhejiang high tech District of Ningbo City Xinhui Road No. 139 Building No. 2 Patentee before: Zhejiang Cellpro Biotech Co., Ltd. |