CN104062168B - A kind of automatic on-line micro-example dilution mixture system and mixed method - Google Patents
A kind of automatic on-line micro-example dilution mixture system and mixed method Download PDFInfo
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- CN104062168B CN104062168B CN201410282025.5A CN201410282025A CN104062168B CN 104062168 B CN104062168 B CN 104062168B CN 201410282025 A CN201410282025 A CN 201410282025A CN 104062168 B CN104062168 B CN 104062168B
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Abstract
The present invention provides a kind of automatic on-line micro-example dilution mixture system and mixed method, and system includes constant flow pump, the first multi-position switching valve, the second multi-position switching valve, blender, six-way valve, quantity tube and sampling pump；First multi-position switching valve includes the first master port and two the first port switchings connected with the first master port selection, and the second multi-position switching valve includes the second master port and four the second port switchings connected with the second master port selection；The two-port of quantity tube and the import of sampling pump are connected on six-way valve, constant flow pump and blender it is connected with in turn between first master port and the second master port, wherein one first port switching is connected with six-way valve, another first port switching is connected with wherein one second port switching, and another second port switching is provided with plug.Realize improving the versatility of automatic on-line micro-example dilution mixture system, meet the dilution mixture requirement of automatic on-line sample.
Description
Technical field
The present invention relates to chromatographic analysis device, particularly relate to a kind of automatic on-line micro-example dilution mixture system and mixed method.
Background technology
Chromatograph application chromatography carries out qualitative and quantitative analysis, and the instrument of the Wuli-Shili-Renli system approach of research material to material.Chromatograph in use, it is generally required to first inject quantitative sample, then injects diluent further according to extension rate and is diluted sample.Generally, after sample mixes with diluent, need to carry out mixing process, prior art generally uses the following two kinds mode: mode one, in the way of stirring, sample and diluent are mixed；Sample and diluent are mixed by mode two, the mode of employing bubbling.Heavy dose of sample and diluent all can sufficiently be mixed by above two mode, but, dilution mixture for automatic on-line micro-example, the concentration of component is in ppb ~ ppm level, its sample size is only between 10 ~ 200 μ L, owing to gathering the dosage of sample seldom (such as: the diluted sample of 10 μ L 300 times), the volume of self of the blender of its correspondence is the least.For mode one, due to the small volume of blender, have no idea to be stirred in a mixer at all；And for mode two, on the one hand limited bubbling effect by blender space poor, on the other hand, the pollutant contained by air that bubbling uses will affect the certainty of measurement of sample.From the foregoing, it will be observed that blender of the prior art cannot meet the dilution mixture requirement of automatic on-line sample.
Summary of the invention
The technical problem to be solved is: provide a kind of automatic on-line micro-example dilution mixture system and mixed method, solve blender in prior art and cannot meet the defect that the dilution mixture of automatic on-line sample requires, realize automatic on-line micro-example dilution mixture system and meet the dilution mixture requirement of automatic on-line sample, improve mixing uniformity.
The technical scheme that the present invention provides is, a kind of automatic on-line micro-example dilution mixture system, including constant flow pump, the first multi-position switching valve, the second multi-position switching valve, blender, six-way valve, quantity tube and sampling pump；Described first multi-position switching valve includes the first master port and two the first port switchings connected with described first master port selection, and described second multi-position switching valve includes the second master port and four the second port switchings connected with described second master port selection；The two-port of described quantity tube and the import of described sampling pump are connected on described six-way valve, it is connected with constant flow pump and blender in turn between described first master port and described second master port, wherein described in one, the first port switching is connected with described six-way valve, another described first port switching is connected with wherein the second port switching described in, and another described second port switching is provided with plug.
Further, described blender includes mixing tube, and described mixing tube is provided with inlet and liquid outlet, and described inlet is connected with described constant flow pump, and described liquid outlet is connected with described second master port.
Further, described mixing tube is additionally provided with air inlet/outlet, described air inlet/outlet is provided with waterproof ventilated membrane.
Further, described blender includes flexible pouch, and described flexible pouch is provided with inlet and liquid outlet, and described inlet is connected with described constant flow pump, and described liquid outlet is connected with described second master port.
Further, described plug seals up another described second port switching.
Further, described plug is additionally provided with air inlet/outlet, and described air inlet/outlet is provided with waterproof ventilated membrane.
Further, described six-way valve is six-way injection valve；Or, described six-way valve includes two two switching valves, described two switching valves include master port and two port switchings connected with the selection of described master port, described quantity tube is connected between two described master ports, and described first multi-position switching valve is corresponding with the described port switching of wherein two switching valves described in respectively with described sampling pump to be connected.
Further, also including waste liquid barrel, the second port switching described in the residue one of described sampling pump and described second multi-position switching valve is connected with described waste liquid barrel respectively.
The present invention also provides for a kind of automatic on-line diluted sample mixed method, uses above-mentioned automatic on-line micro-example dilution mixture system, and concrete grammar includes:
Step one, sample introduction: sample is filled quantity tube by sampling pump；
Step 2, dilution: by constant flow pump by the sample delivery in quantity tube to blender, and diluent is transported in blender simultaneously；
Step 3, mixing: constant flow pump drives the mixed liquor of sample and diluent to circulate between blender and constant flow pump.
Preferably, described step 3 is particularly as follows: constant flow pump alternately rotates and reverse, to drive the mixed liquor of sample and diluent to circulate between blender and constant flow pump.
nullThe automatic on-line micro-example dilution mixture system of present invention offer and mixed method，By using quantity tube quantitatively to measure the sample of trace，Owing to the volume of quantity tube is fixing，The sample of trace can be measured in high precision accurately，And use constant flow pump to be diluted mixing，Constant flow pump plays dilution and two kinds of effects of mixing in the course of the work，Constant flow pump is by setting its operation time and flow，Accurately sample can be set the dilution of multiple，And when mixing，The mixed liquor being driven sample and diluent by constant flow pump is circulated between blender and constant flow pump，So that sample and the sufficient mix homogeneously of diluent，And，In mixed process，The content of material of mixed liquor itself will not be produced impact，Can effectively meet the requirement that low dose of sample carries out mixing in the blender of small volume，Realize online micro-example dilution mixture system and meet the dilution mixture requirement of automatic on-line sample，Improve mixing uniformity.
Accompanying drawing explanation
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, the accompanying drawing used required in embodiment or description of the prior art will be briefly described below, apparently, accompanying drawing in describing below is some embodiments of the present invention, for those of ordinary skill in the art, on the premise of not paying creative work, it is also possible to obtain other accompanying drawing according to these accompanying drawings.
Fig. 1 is the structural representation that automatic on-line micro-example dilution mixture system embodiment one of the present invention is in sample introduction state；
Fig. 2 is the structural representation that automatic on-line micro-example dilution mixture system embodiment one of the present invention is in diluted state；
Fig. 3 is the structural representation that automatic on-line micro-example dilution mixture system embodiment one of the present invention is in admixture；
Fig. 4 is the structural representation of automatic on-line micro-example dilution mixture system embodiment two of the present invention；
Fig. 5 is the structural representation of blender in automatic on-line micro-example dilution mixture system of the present invention.
Detailed description of the invention
For making the purpose of the embodiment of the present invention, technical scheme and advantage clearer, below in conjunction with the accompanying drawing in the embodiment of the present invention, technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is a part of embodiment of the present invention rather than whole embodiments.Based on the embodiment in the present invention, the every other embodiment that those of ordinary skill in the art are obtained under not making creative work premise, broadly fall into the scope of protection of the invention.
Embodiment one
As it is shown in figure 1, the present embodiment automatic on-line micro-example dilution mixture system, including constant flow pump the 1, first multi-position switching valve the 2, second multi-position switching valve 3, blender 4, six-way valve 8, quantity tube 5 and sampling pump 7；First multi-position switching valve includes that the first master port 21 selects the first port switching 22,23 connected with two with the first master port 21, and the second multi-position switching valve 3 includes that the second master port 31 selects the second port switching 32,33,34,35 connected with four with the second master port 31；The two-port of quantity tube 5 and the import of sampling pump 7 are connected on six-way valve 8, it is connected with constant flow pump 1 and blender 4 in turn between first master port 21 and the second master port 31, wherein one first port switching 22 is connected with six-way valve 8, another first port switching 23 is connected with wherein one second port switching 35, and another second port switching 33 is provided with plug 331.
Specifically, the present embodiment automatic on-line micro-example dilution mixture system can effectively carry out the sample introduction of micro-example, the quantity tube 5 connected on six-way valve 8 can be designed as setting micro-scale volume as required, when carrying out sample introduction by sampling pump 7, as shown in Figure 1, six-way valve 8 connects sample, quantity tube 5 and sampling pump 7 stream, will fill sample in quantity tube 5.And use the sample and outside diluent that constant flow pump 1 extracts in quantity tube 5, and by setting operation time and the flow of constant flow pump 1, the multiple that can accurately diluted sample be set, it is achieved thereby that the transmission of high-precision liquid.Constant flow pump 1 in the present embodiment has two kinds of functions: dilutes and mixes.Concrete, first, as shown in Figure 2, six-way valve 8 connects diluent, quantity tube 5 and the first multi-position switching valve 2 stream, first master port 21 and connecting with the first port switching 22, second master port 31 connects with being connected the second port switching 33 having plug 331, and constant flow pump 1 can extract the sample in quantity tube 5 and diluent by the first port switching 22, is transported in blender 4 by the mixed liquor of sample and diluent；Then, as shown in Figure 3, first master port 21 and connecting with the first port switching 23, second master port 31 connects with the second port switching 35, circulation stream is formed between constant flow pump 1 and blender 4, constant flow pump 1 runs to drive mixed liquor to circulate, and mixed liquor is during circulating so that sample and diluent are sufficiently mixed uniformly.While the present embodiment automatic on-line micro-example dilution mixture system disclosure satisfy that common sample introduction dilution mixture, additionally it is possible to well meet the dilution mixture requirement of automatic on-line micro-example.
It addition, after sample in blender 4 and diluent mix homogeneously, the second master port 31 selects connection the second port switching 32, and the mixed liquor of mix homogeneously just can be transported in analytical tool by constant flow pump 1.Simultaneously, cleanout fluid can also be entered in blender 4 in the way of above-mentioned mixed process is identical and be carried out by constant flow pump 1, after blender 4 cleans up, second master port 31 selects connection the second port switching 34, connecting on 3rd port switching 34 has waste liquid barrel 6, the cleanout fluid in blender 4 to automatically flow in waste liquid barrel 6.Meanwhile, the outlet of sampling pump 7 is also connected with waste liquid barrel 6, and when sample introduction, too much sample enters in waste liquid barrel 6.
nullThe present embodiment automatic on-line micro-example dilution mixture system，By using quantity tube quantitatively to measure the sample of trace，Owing to the volume of quantity tube is fixing，The sample of trace can be measured in high precision accurately，And use constant flow pump to be diluted mixing，Constant flow pump plays dilution and two kinds of effects of mixing in the course of the work，Constant flow pump is by setting its operation time and flow，Accurately sample can be set the dilution of multiple，And when mixing，The mixed liquor being driven sample and diluent by constant flow pump is circulated between blender and constant flow pump，So that sample and the sufficient mix homogeneously of diluent，And，In mixed process，The content of material of mixed liquor itself will not be produced impact，Can effectively meet the requirement that low dose of sample carries out mixing in the blender of small volume，Realize online micro-example dilution mixture system and meet the dilution mixture requirement of automatic on-line sample，Improve mixing uniformity.
Embodiment two
The present embodiment automatic on-line micro-example dilution mixture system is based on above-described embodiment one, difference is: as it is shown in figure 5, blender 4 includes mixing tube 41, mixing tube 41 is provided with inlet 42 and liquid outlet 43, inlet 42 is connected with constant flow pump 1, and liquid outlet 43 is connected with the second master port 31.Concrete, blender 4 is mainly made up of mixing tube 41, diluent is injected in mixing tube 41 by constant flow pump 1, and in dilution, so that constant flow pump 1 can be happy mixing liquid is injected in mixing tube 41, plug 331 is additionally provided with air inlet/outlet (not shown), waterproof ventilated membrane (not shown) it is provided with on described air inlet/outlet, during diluent is injected into mixing tube 41 by constant flow pump 1, air in mixing tube 41 is discharged through the air inlet/outlet of plug 331, and owing to being provided with waterproof ventilated membrane, the liquid in mixing tube 41 can be avoided to leak into outside.Preferably, mixing tube 41 is additionally provided with air inlet/outlet (not shown), air inlet/outlet is provided with waterproof ventilated membrane 44.Concrete, waterproof ventilated membrane 44 has ventilative and waterproof function, owing to the liquid outlet 43 of mixing tube 41 is connected with second port switching 33 with plug 331 by the second master port 31, plug 331 now completely seals off the second port switching 33, make the second port switching 33 airtight the most waterproof, during sample and diluent are transported to mixing tube 41 by constant flow pump 1, waterproof ventilated membrane 44 can balance the pressure in mixing tube 41, to guarantee that constant flow pump 1 runs smoothly, simultaneously, waterproof ventilated membrane 44 can effectively filter the pollutant entered in mixing tube 41 contained by gas, guarantee the certainty of measurement of sample.
Embodiment three
The present embodiment automatic on-line micro-example dilution mixture system is based on above-described embodiment one, and difference is: blender includes flexible pouch, and flexible pouch is provided with inlet and liquid outlet, and inlet is connected with constant flow pump 1, and liquid outlet and the second master port 31 connect.Concrete, blender uses the mode of flexible pouch, due to flexible pouch deformable, such that it is able to carry out the change adapted to according to the number of flexible pouch internal mix liquid, to guarantee during sample and diluent are transported to mixing tube 41 by constant flow pump 1, constant flow pump 1 can run smoothly, and plug 331 now completely seals off the second port switching 33 so that the second port switching 33 is airtight the most waterproof.Further, since flexible pouch is hermetically sealed, do not have the impact of environmental contaminants, it is ensured that the certainty of measurement of sample.
Embodiment four
The present embodiment automatic on-line micro-example dilution mixture system is based on above-described embodiment one, and difference is: as shown in Figure 4, and six-way valve 8 is six-way injection valve.Or, as it is shown in figure 1, six-way valve 8 includes that two two switching valves 81,82, two switching valves 81,82 include that master port (not shown) selects the port switching (not shown) connected with two with master port, quantity tube 5 is connected between two master ports；First multi-position switching valve 2 is corresponding with the port switching of wherein one or two switching valve 82 respectively with sampling pump 7 to be connected.
Embodiment five
The present invention provides again a kind of automatic on-line diluted sample mixed method, uses above-mentioned automatic on-line micro-example dilution mixture system, and as a example by the automatic on-line micro-example dilution mixture system using embodiment one, as shown in Figure 1-Figure 3, concrete grammar includes:
Step one, sample introduction: sample filled quantity tube 5 by sampling pump 7.Concrete, as it is shown in figure 1, six-way valve 8 connects sample, quantity tube 5 and sampling pump 7 stream, sampling pump 7 extracts sample so that will fill sample in quantity tube 5.
Step 2, dilution: by constant flow pump 1 by the sample delivery of quantity tube 5 to blender 4, and diluent is transported in blender 4 simultaneously.Concrete, as shown in Figure 2, six-way valve 8 connects diluent, quantity tube 5 and the first multi-position switching valve 2 stream, second master port 31 connects with being connected the second port switching 33 having plug 331, first master port 21 and connecting with the first port switching 22, constant flow pump 1 can extract the sample in quantity tube 5 and a certain amount of diluent by the first port switching 22 so that sample is diluted with the extension rate of regulation.
Step 3, mixing: constant flow pump 1 drives the mixed liquor of sample and diluent to circulate between blender 4 and constant flow pump 1.Concrete, as shown in Figure 3, first master port 21 and connecting with the first port switching 23, second master port 31 connects with the second port switching 35, circulation stream is formed between constant flow pump 1 and blender 4, constant flow pump 1 runs to drive mixed liquor to circulate, and mixed liquor is during circulating so that sample and diluent are sufficiently mixed uniformly.Preferably, constant flow pump 1 can alternately rotate and reverse, so that after mixed liquor circulates a period of time between blender 4 and constant flow pump 1, then reverse cycle flow, with more effective mix homogeneously mixed liquor.
nullThe present embodiment automatic on-line diluted sample mixed method，By using quantity tube quantitatively to measure the sample of trace，Owing to the volume of quantity tube is fixing，The sample of trace can be measured in high precision accurately，And use constant flow pump to be diluted mixing，Constant flow pump plays dilution and two kinds of effects of mixing in the course of the work，Constant flow pump is by setting its operation time and flow，Accurately sample can be set the dilution of multiple，And when mixing，The mixed liquor being driven sample and diluent by constant flow pump is circulated between blender and constant flow pump，So that sample and the sufficient mix homogeneously of diluent，And，In mixed process，The content of material of mixed liquor itself will not be produced impact，Can effectively meet the requirement that low dose of sample carries out mixing in the blender of small volume，Realize online micro-example dilution mixture system and meet the dilution mixture requirement of automatic on-line sample，Improve mixing uniformity.
Last it is noted that above example is only in order to illustrate technical scheme, it is not intended to limit；Although the present invention being described in detail with reference to previous embodiment, it will be understood by those within the art that: the technical scheme described in foregoing embodiments still can be modified by it, or wherein portion of techniques feature is carried out equivalent；And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of various embodiments of the present invention technical scheme.
Claims (10)
1. an automatic on-line micro-example dilution mixture system, it is characterised in that include constant flow pump, the first multi-position switching valve, the second multi-position switching valve, blender, six-way valve, quantity tube and sampling pump；Described first multi-position switching valve includes the first master port and two the first port switchings connected with described first master port selection, and described second multi-position switching valve includes the second master port and four the second port switchings connected with described second master port selection；The two-port of described quantity tube and the import of described sampling pump are connected on described six-way valve, it is connected with constant flow pump and blender in turn between described first master port and described second master port, wherein described in one, the first port switching is connected with described six-way valve, another described first port switching is connected with wherein the second port switching described in, and another described second port switching is provided with plug.
Automatic on-line micro-example dilution mixture system the most according to claim 1, it is characterized in that, described blender includes mixing tube, and described mixing tube is provided with inlet and liquid outlet, described inlet is connected with described constant flow pump, and described liquid outlet is connected with described second master port.
Automatic on-line micro-example dilution mixture system the most according to claim 2, it is characterised in that be additionally provided with air inlet/outlet on described mixing tube, described air inlet/outlet is provided with waterproof ventilated membrane.
Automatic on-line micro-example dilution mixture system the most according to claim 1, it is characterized in that, described blender includes flexible pouch, and described flexible pouch is provided with inlet and liquid outlet, described inlet is connected with described constant flow pump, and described liquid outlet is connected with described second master port.
5. according to the automatic on-line micro-example dilution mixture system described in claim 3 or 4, it is characterised in that described plug seals up another described second port switching.
Automatic on-line micro-example dilution mixture system the most according to claim 2, it is characterised in that described plug is additionally provided with air inlet/outlet, and described air inlet/outlet is provided with waterproof ventilated membrane.
Automatic on-line micro-example dilution mixture system the most according to claim 1, it is characterised in that described six-way valve is six-way injection valve；Or, described six-way valve includes two two switching valves, described two switching valves include master port and two port switchings connected with the selection of described master port, described quantity tube is connected between two described master ports, and described first multi-position switching valve is corresponding with the described port switching of wherein two switching valves described in respectively with described sampling pump to be connected.
Automatic on-line micro-example dilution mixture system the most according to claim 1, it is characterised in that also include waste liquid barrel, the second port switching described in the residue one of described sampling pump and described second multi-position switching valve is connected with described waste liquid barrel respectively.
9. an automatic on-line diluted sample mixed method, it is characterised in that using the automatic on-line micro-example dilution mixture system as described in claim 1-8 is arbitrary, concrete grammar includes:
Step one, sample introduction: sample is filled quantity tube by sampling pump；
Step 2, dilution: by constant flow pump by the sample delivery in quantity tube to blender, and diluent is transported in blender simultaneously；
Step 3, mixing: constant flow pump drives the mixed liquor of sample and diluent to circulate between blender and constant flow pump.
Automatic on-line diluted sample mixed method the most according to claim 9, it is characterised in that described step 3 is particularly as follows: constant flow pump alternately rotates and reverse, to drive the mixed liquor of sample and diluent to circulate between blender and constant flow pump.
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| CN107560923B (en) * | 2017-08-24 | 2020-08-04 | 天津市华宇膜技术有限公司 | Water sample dilution system and method for expanding online water quality detection range |
| CN111521478B (en) * | 2020-05-20 | 2023-04-18 | 中国科学院深海科学与工程研究所 | Deep sea normal position gradient circulation diluting device |
| CN112525660A (en) * | 2020-11-12 | 2021-03-19 | 核工业北京化工冶金研究院 | Automatic sampling, sample reserving and concentration proportioning device based on micro volume |
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| CN201364291Y (en) * | 2008-12-18 | 2009-12-16 | 杭州鼎利环保科技有限公司 | On-line constant-volume diluter |
| EP2920596A1 (en) * | 2012-11-15 | 2015-09-23 | Agilent Technologies, Inc. | Sample dilution to specifiable dilution ratio |
| CN103398894B (en) * | 2013-06-17 | 2016-01-06 | 孙卫国 | A kind of air mix facilities of proportion adjustable and dilution process |
| CN103411910B (en) * | 2013-08-13 | 2015-09-09 | 杭州派析光电科技有限公司 | The online ultraviolet spectrum detection method of extraction of traditional Chinese medicine and system |
| CN103785314B (en) * | 2014-03-04 | 2016-05-11 | 厦门大学 | A kind of blender and flow type photometric detection automated analysis instrument |
| CN203908853U (en) * | 2014-06-23 | 2014-10-29 | 青岛普仁仪器有限公司 | Automatic online micro-sample diluting and mixing system |
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