CN104062165A

CN104062165A - Anti-dropping agent for slices, and preparation method and usage of anti-dropping agent

Info

Publication number: CN104062165A
Application number: CN201410270745.XA
Authority: CN
Inventors: 王艳萍; 廖秋萍; 石长青; 曾维斌
Original assignee: Tarim University
Current assignee: Tarim University
Priority date: 2014-06-17
Filing date: 2014-06-17
Publication date: 2014-09-24

Abstract

The invention discloses an anti-dropping agent for slices. The anti-dropping agent comprises egg white of an egg, glycerol and ethanol which are uniformly mixed. The anti-dropping agent for the slices can be stably bonded with glass slides, so that the defect that in experiments, especially tissue slice immunohistochemical experiments, the tissue slice is easy to drop as the tissue is heated by high temperature and is soaked in liquid for a long time is overcome. Compared with an existing anti-dropping agent for the slices, the anti-dropping agent for the slices has the stronger adhesive capacity, can enable the slices not to drop, can remove red dye of a conventional egg white and glycerin adhesive agent, and improves the resolution.

Description

Section anticreep agent and preparation and application thereof

Technical field

The present invention relates to the agent of a kind of section anticreep, belong to Bioexperiment science equipment field.

Background technology

In the scientific experiment of science and engineering class, especially in biological species scientific experiment, the scientific experiment of pathology class, for the ease of micro-Microscopic observation or sample fix, during the purposes such as dyeing, SABC, affected by the various external factor such as high temperature, high pressure, radiation, acid and alkali corrosion, histocyte very easily comes off from microslide.Existing slide normally adopts water or adhesive to process, the problem that adopts water is bonding insecure, to be existing adhesive is formed and is caused that slide background is painted, pollution by common albumen and glycerine the problem that adopts adhesive, red coloration dyeing background normally, not only influence technique personnel's routine work, is also unfavorable for the purposes such as diagnosis of pathology.

Summary of the invention

For problems of the prior art, the invention discloses the agent of a kind of section anticreep, can be by cell, the section of tissue etc. adheres on microslide securely, prevent from coming off, overcome in experimentation, especially in histotomy immunohistochemical experiment because tissue is subject in high temperature and liquid soaking for a long time the as easy as rolling off a log defect of falling sheet, compare with existing anti-flake, section anticreep agent adhesive capacity of the present invention is stronger, can not fall sheet, and section anticreep of the present invention agent can be removed the red staining background of traditional albumen glycerine binder, strengthen resolving power, do not affect diagnostic observation.

For achieving the above object, the present invention is achieved through the following technical solutions:

The agent of section anticreep, comprises the following compositions mixing: the egg white of egg, glycerine, little Molecular Organic Alcohol.

Wherein, above-mentioned each composition is liquid state, can adjust according to actual needs the consumption of each composition, conventionally in volume ratio, and the egg white that the consumption of each composition is egg: glycerine: ethanol=(0.5-2): (0.1-1.5): (0.5-2).

Preferably, the egg white that the consumption of each composition is egg: glycerine: ethanol=(1-1.5): (0.8-1.2): (1-1.5).

Section anticreep of the present invention agent, utilize the multiple protein composition in egg white, mainly ovalbumin, ovomucoid, ovomucin etc., glass surface microstructure is played to chemical modification effect, thereby the physics and chemistry activity on change glass baseplate surface forms stable integrated structure, make section more firm with the combination of microslide; Meanwhile, by glycerine, the synergy of little Molecular Organic Alcohol, improves the affinity of microslide, increases liquid rate of propagation, and the cell that makes to cut into slices is even, single is distributed in viewing area; Meanwhile, utilize the mutual mixed effect of three, eliminated the red staining background of traditional adhesive, guaranteed the accuracy of observing.

In the present invention, little Molecular Organic Alcohol used is a kind of in ethanol, n-propanol or isopropyl alcohol, and preferred, little Molecular Organic Alcohol is ethanol, is generally low-concentration ethanol, more preferably 40% ethanol.

Accordingly, the invention discloses the preparation method of described section anticreep agent, comprise the steps:

1) separated egg white from egg, stirs;

2) egg white filtration is rear and glycerine mixes;

3) add little Molecular Organic Alcohol, after mixing, pour stored refrigerated in drop bottle into.

For the ease of identification and mark, above-mentioned drop bottle can be labelled, signature and date etc.

Finally, the invention also discloses the using method of section anticreep agent, drip on microslide, then drip distilled water by the anticreep agent of cutting into slices, the two is attached to another slide to drip on the microslide that has the agent of section anticreep after mixing, and friction evenly mutually.

Wherein, the consumption of distilled water, the agent of section anticreep can be selected according to actual needs, and generally speaking the volumetric usage of the two is identical.

In order to eliminate the external contaminations such as pathogenic microorganism, and remove unnecessary water stainly, above-mentioned using method also comprises the step of the microslide heated baking having sticked.

Embodiment

Embodiment 1: the preparation of section anticreep agent

1), break egg, the separated egg white that takes out, stirs with glass bar.

2), put into graduated cylinder after filtering with double-deck qualitative filter paper, then add isopyknic glycerine, mix.

3), to add volume be 40% ethanol of egg white and glycerine volume and 2/3, mix.

4), pour in 30ml drop bottle.Labelled, signature and date.Refrigerator cold-storage is preserved.

Embodiment 2: the preparation of section anticreep agent

1), break egg, the separated egg white that takes out, stirs with glass bar.

2), put into graduated cylinder after filtering with double-deck qualitative filter paper, then add the glycerine of 1.1 times of volumes, mix.

3), to add volume be 40% ethanol of egg white and glycerine volume and 0.7 times, mix.

Embodiment 3: the preparation of section anticreep agent

1), break egg, the separated egg white that takes out, stirs with glass bar.

2), with putting into graduated cylinder after Filter paper filtering, then add the glycerine of 0.85 times of volume, mix.

3), to add volume be 55% ethanol of egg white and glycerine volume and 0.6 times, mix.

Embodiment 4: the preparation of section anticreep agent

1), break egg, the separated egg white that takes out, stirs with glass bar.

2), egg white puts into graduated cylinder after filtering, then adds the glycerine of 1.3 times of volumes, mix.

3), to add volume be the n-propanol of egg white and glycerine volume and 0.9 times, mix.

Above-mentioned section anticreep agent is used by the following method: with dropper, drip on microslide, then drip distilled water, the two mixes; Another slide is attached to drip to be had on the slide of binder, mutually friction evenly, the like, put into the roasting sheet 1-2 hour of 50 degree baking ovens after making multiple sheets.

Utilize anticreep agent of the present invention, process 500 microslides, carry out cytology, histology film-making, various film-making cells fall sheet rate and are 0%, simultaneously for various sectioning cells, be solid-state or liquid base all maintains good affinity, surface of glass slide is very evenly clean, microslide adhesion property is splendid, and in whole experiment observation process, does not all occur the red staining background interference phenomenon of traditional common albumen glycerine binder.Existing common albumen glycerine binder not only exists red background to disturb, more crucial is that this binder is very poor for liquid basal cell section affinity and adhesiveness, there is the higher sheet rate of falling, and adopt common albumen glycerine binder surface of glass slide Microscopic observation can find that surface is uneven, cleanliness are bad.

Claims

1. the agent of section anticreep, is characterized in that comprising the following compositions mixing: the egg white of egg, glycerine, little Molecular Organic Alcohol.

2. according to the section anticreep agent of claim 1, it is characterized in that in volume ratio the egg white that the consumption of each composition is egg: glycerine: ethanol=(0.5-2): (0.1-1.5): (0.5-2).

3. according to the section anticreep agent of claim 1, it is characterized in that little Molecular Organic Alcohol is a kind of in ethanol, n-propanol or isopropyl alcohol.

4. according to the section anticreep agent of claim 3, it is characterized in that little Molecular Organic Alcohol is 40% ethanol.

5. the preparation method of the section anticreep agent of claim 1, is characterized in that comprising the steps: 1) separated egg white from egg, stir; 2) egg white filtration is rear and glycerine mixes; 3) add little Molecular Organic Alcohol, after mixing, pour stored refrigerated in drop bottle into.

6. the using method of the section anticreep agent of claim 1, is characterized in that the agent of section anticreep to drip on microslide, then drips distilled water, and the two is attached to another slide to drip on the microslide that has the agent of section anticreep after mixing, and friction evenly mutually.

7. according to the using method of claim 6, it is characterized in that described distilled water is identical with section anticreep agent volume.

8. according to the using method of claim 6, characterized by further comprising the step of the microslide heated baking having sticked.