CN105004594A - Fixing method and use of tissue slice - Google Patents
Fixing method and use of tissue slice Download PDFInfo
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- CN105004594A CN105004594A CN201510419068.8A CN201510419068A CN105004594A CN 105004594 A CN105004594 A CN 105004594A CN 201510419068 A CN201510419068 A CN 201510419068A CN 105004594 A CN105004594 A CN 105004594A
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- microslide
- histotomy
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- drying
- fixing means
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Abstract
The invention provides a fixing method and a use of a tissue slice; a quick-drying glue is rapidly dropped on a glass slide and evenly smeared into a thin layer, a tissue slice rapidly adheres onto the glass slide kiss-coated hung with the quick-drying glue, the tissue slice is closely bonded with the glass slide, and a slice falling-off phenomenon almost cannot happen; the operation is simple, the used glass slide is cheaper than a slice with a glue on markets, and the tissue slice can be used for HE staining, immunohistochemistry and various staining.
Description
Technical field
The invention belongs to biological technical field, be specifically related to pathological section and adhere to dropping-prevention piece method.
Background technology
It is inevitable problem in pathological staining process all the time that histotomy comes off.The method of the pre-dropping-prevention piece of current existing section mainly comprises collodion coating, poly-D-lysine and chrome alum gelatin process slide etc.Although hard tissue slicing also can prevent sheet, its section blocked up (about 40 ~ 100 μm), organizes overlap not easily to observe, cannot carry out pathological staining.Paraffin and frozen section can reach within 10 μm, can observation of cell form preferably, but there are some all the time and organize paster loosely, the problem of sheet is easily fallen when dyeing, especially, in the section statining of the tissue engineering material such as silk and artificial ligament, sheet phenomenon is fallen more outstanding.Simultaneously in the dyeing such as HE, Ponceaux three look and SABC, because staining procedure is loaded down with trivial details, organize the reasons such as long soaking time, it is particularly serious that tissue falls sheet phenomenon.
Summary of the invention
The object of the present invention is to provide fixing means of a kind of histotomy and uses thereof.
For achieving the above object, present invention employs following technical scheme.
A fixing means for histotomy, comprises the following steps:
1) experiment to be drawn materials or pathologic specimen paraformaldehyde is fixed, if experiment is drawn materials or pathologic specimen is bone tissue, then after paraformaldehyde is fixing, carry out decalcification process;
2) by through step 1) experiment after process is drawn materials or pathologic specimen gum arabic solution soaks 24 ~ 48 hours, and be then switched to 5 ~ 10 μm with freezing microtome, obtain histotomy;
3) quick-drying gelatin is evenly coated on microslide, then histotomy is fixed on microslide on the region of evenly coating quick-drying gelatin, then natural air drying or with roasting sheet machine in 55 ~ 65 DEG C of roasting sheets 1 ~ 2 hour.
Described tissue section sampling is drawn materials or pathologic specimen from all experiments easily falling sheet.
Described experiment is drawn materials or pathologic specimen is silk organizational project ligament.
Described being evenly coated on microslide by quick-drying gelatin specifically comprises the following steps: drip some quick-drying gelatins on microslide A, then cover above microslide A with microslide B immediately, the quick-drying gelatin level dropped on microslide A is sprawled, thus quick-drying gelatin is evenly coated on microslide A and microslide B.
The purposes of fixing means in tissue staining of above-mentioned histotomy.
Described histotomy is used for HE dyeing, Ponceaux trichrome stain or immunohistochemical staining.
Described tissue section sampling is in silk organizational project ligament.
Beneficial effect of the present invention is embodied in:
The present invention is on common microslide after even coating film quick-drying gelatin, by frozen tissue section paster, histotomy is combined with microslide closely, and it is easy to make, simple to operate, need the time to be processed short, cost is low, effectively prevent occur in dyeing course sheet phenomenon, what thoroughly solve pathological staining falls sheet problem, be particularly useful for the section statining of caducous silk material, organizational project ligament or other tissue engineering materials, and it is blocked up to efficiently solve hard tissue slicing, cannot the isostructural problem of clear showed cell.
Accompanying drawing explanation
Fig. 1 is for cutting into slices and carry out HE coloration result fixing silk ligament on chrome alum gelatin process slide;
Fig. 2 fixes silk ligament cut into slices and carry out HE coloration result on the slide of coating quick-drying gelatin.
Embodiment
Below in conjunction with drawings and Examples, the present invention is elaborated.
Paraformaldehyde of learning from else's experience is fixed, the silk ligament tissue of decalcification, use gum arabic solution (containing massfraction 10% gum arabic and 30% the aqueous solution of sucrose) soak 24 ~ 48 hours, obtain sample, freezing microtome is utilized sample to be cut into 5 ~ 10 μm of thick histotomies, common microslide drips 1 quick-drying gelatin (such as, Yiwu City Lv Jie commodity factory, elephant stuck glue water), an other microslide is utilized to adopt the method for rapid compressing tablet, quick-drying gelatin is coated on equably on two panels microslide, rapid tissue being adhered to wherein a slice microslide applies on the region of quick-drying gelatin again, then (60 DEG C) roasting sheet 1 ~ 2 hour on natural air drying or roasting sheet machine.Control group uses through the section of chrome alum gelatin process slide adhesion organization, carries out HE dyeing simultaneously.
Sheet serious (chrome alum gelatin process slide) is fallen see tissue after visible stain in Fig. 1 and Fig. 2, Fig. 1; Dye in Fig. 2 color and luster normal (slide of the inventive method process), almost without falling sheet phenomenon.
Histotomy fixing means of the present invention can effectively be avoided falling sheet (flake) phenomenon in histological staining process.
Claims (7)
1. a fixing means for histotomy, is characterized in that: comprise the following steps:
1) experiment to be drawn materials or pathologic specimen paraformaldehyde is fixed, if experiment is drawn materials or pathologic specimen is bone tissue, then after paraformaldehyde is fixing, carry out decalcification process;
2) by through step 1) experiment after process is drawn materials or pathologic specimen gum arabic solution soaks 24 ~ 48 hours, and be then switched to 5 ~ 10 μm with freezing microtome, obtain histotomy;
3) quick-drying gelatin is evenly coated on microslide, then histotomy is fixed on microslide on the region of evenly coating quick-drying gelatin, then natural air drying or with roasting sheet machine in 55 ~ 65 DEG C of roasting sheets 1 ~ 2 hour.
2. the fixing means of a kind of histotomy according to claim 1, is characterized in that: described tissue section sampling is drawn materials or pathologic specimen from all experiments easily falling sheet.
3. the fixing means of a kind of histotomy according to claim 1, is characterized in that: described experiment is drawn materials or pathologic specimen is silk organizational project ligament.
4. the fixing means of a kind of histotomy according to claim 1, it is characterized in that: described being evenly coated on microslide by quick-drying gelatin specifically comprises the following steps: drip some quick-drying gelatins on microslide A, then cover above microslide A with microslide B immediately, the quick-drying gelatin level dropped on microslide A is sprawled, thus quick-drying gelatin is evenly coated on microslide A and microslide B.
5. the purposes of fixing means in tissue staining of a histotomy as claimed in claim 1.
6. purposes according to claim 5, is characterized in that: described histotomy is used for HE dyeing, Ponceaux trichrome stain or immunohistochemical staining.
7. purposes according to claim 5, is characterized in that: described tissue section sampling is in silk organizational project ligament.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110377940.2A CN113092221A (en) | 2015-07-16 | 2015-07-16 | Kit for fixing tissue slices and application thereof |
| CN201510419068.8A CN105004594A (en) | 2015-07-16 | 2015-07-16 | Fixing method and use of tissue slice |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510419068.8A CN105004594A (en) | 2015-07-16 | 2015-07-16 | Fixing method and use of tissue slice |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110377940.2A Division CN113092221A (en) | 2015-07-16 | 2015-07-16 | Kit for fixing tissue slices and application thereof |
Publications (1)
| Publication Number | Publication Date |
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| CN105004594A true CN105004594A (en) | 2015-10-28 |
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| CN201510419068.8A Pending CN105004594A (en) | 2015-07-16 | 2015-07-16 | Fixing method and use of tissue slice |
| CN202110377940.2A Pending CN113092221A (en) | 2015-07-16 | 2015-07-16 | Kit for fixing tissue slices and application thereof |
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| CN202110377940.2A Pending CN113092221A (en) | 2015-07-16 | 2015-07-16 | Kit for fixing tissue slices and application thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108651438A (en) * | 2018-05-02 | 2018-10-16 | 济南天灿医疗科技有限公司 | A kind of tissue for tissue block fixes agents for defoliating and application |
| CN111238901A (en) * | 2020-03-04 | 2020-06-05 | 四川省人民医院 | Manufacturing method of improved mouse eyeball frozen section |
| CN112985952A (en) * | 2021-02-20 | 2021-06-18 | 山东骏腾医疗科技有限公司 | Novel pathological sealing sheet material |
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| CN103471903A (en) * | 2013-09-29 | 2013-12-25 | 吴天骥 | Method for preparing stationary liquid for slicing and application of stationary liquid for slicing |
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2015
- 2015-07-16 CN CN201510419068.8A patent/CN105004594A/en active Pending
- 2015-07-16 CN CN202110377940.2A patent/CN113092221A/en active Pending
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| CN104634626A (en) * | 2013-11-15 | 2015-05-20 | 上海交通大学 | Improved frozen slicing method and applications thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108651438A (en) * | 2018-05-02 | 2018-10-16 | 济南天灿医疗科技有限公司 | A kind of tissue for tissue block fixes agents for defoliating and application |
| CN111238901A (en) * | 2020-03-04 | 2020-06-05 | 四川省人民医院 | Manufacturing method of improved mouse eyeball frozen section |
| CN111238901B (en) * | 2020-03-04 | 2023-03-03 | 四川省人民医院 | Manufacturing method of improved mouse eyeball frozen section |
| CN112985952A (en) * | 2021-02-20 | 2021-06-18 | 山东骏腾医疗科技有限公司 | Novel pathological sealing sheet material |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113092221A (en) | 2021-07-09 |
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