CN104059951A - Extraction method of natural anthocyanidin active aglycone - Google Patents
Extraction method of natural anthocyanidin active aglycone Download PDFInfo
- Publication number
- CN104059951A CN104059951A CN201310112792.7A CN201310112792A CN104059951A CN 104059951 A CN104059951 A CN 104059951A CN 201310112792 A CN201310112792 A CN 201310112792A CN 104059951 A CN104059951 A CN 104059951A
- Authority
- CN
- China
- Prior art keywords
- anthocyanidin
- revs
- aerobic bacteria
- water
- mentioned
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to an extraction method of natural anthocyanidin active aglycone, which belongs to the technical field of bioengineering. The method comprises the following steps: performing classification, crushing, water absorption, freezing, heating for unfreezing, and filtering for juice preparation of anthocyanidin-containing plants, performing biodegradation of anthocyanin by glycosidase for decomposing anthocyanin glucosidic bonds, performing combination reaction precipitation/suspension by an anionic surfactant, degrading the precipitates/suspensions by bacterial microbe, and performing sterilization, enzyme and bacterium filtration, and freeze drying of the anthocyanidin aqueous solution. The method of the invention can obtain cationic anthocyanidin, instead of anthocyanin, with a real content of more than 95%. The cationic anthocyanidin prepared by the method of the invention needs no degradation by microbe in human body intestinal tracts, can be absorbed and used directly by human body, or can directly enter human body blood, and is applicable to medicine and health care.
Description
Affiliated technical field:
The present invention relates to the extracting method of a kind of plant positively charged ion anthocyanidin of technical field of bioengineering, the extracting method of especially natural anthocyanidin active aglycone.
Background technology:
Along with the raising of people's living standard with to nontoxic being conducive in natural plant source, protect that people are healthy, the pursuit of the natural organic substance of preventing and curing diseases; simultaneously also because synthetic food color has larger toxic side effect to human body; the Yi Bei World Health Organization bans use of; again because anthocyanidin industrial synthesis investment is large, technical sophistication is unstable, poor product quality, and cannot serve for the mankind.Therefore natural positively charged ion anthocyanidin active aglycone and the extracting method thereof of high effect nontoxic have become emerging Some Questions To Be Researched of 21 century.
Existing but " anthocyanidin " separation and extraction technology (except Chinese patent application 200910185829.2) just obtains the anthocyanogen containing anthocyanidin, and content is too low, impurity is too many, so it is used in the painted of food dyeing and makeup mostly, and value medical health care is low.Existing ultrasonic wave, microbiological deterioration are destroyed plant tissue cell's wall, the anthocyanogen that extraction waits separating and purifying technology to extract to obtain with really to human body have health care, the positively charged ion anthocyanidin acting on of preventing and curing diseases is essentially different.Anthocyanogen be in plant materials positively charged ion anthocyanidin to be pattern primitive be combined the glucosides of formation with various monose by glycosidic link.This is a kind of neutral substance, biological activity is low, in human body, only have and by human enteric bacteria, degrade that to form positively charged ion anthocyanidin be the utilization that just can be absorbed by the body of anthocyanidin active aglycone in desugar, eliminate harmful free radical in human body, prevent and cure diseases, but the anthocyanogen of this neutrality is degraded in human body, metabolic rate is very low, and bioavailability is low, thereby human body is also very low to its specific absorption.
The individual bio-transformation function difference of adding people is very large, and particularly the elderly and patient's enteron aisle is lower to the degraded and absorbed degree of anthocyanogen.Thereby the outer degraded of industrialization plant anthocyanogen of human body and the separation and Extraction of the positively charged ion anthocyanidin particularly important that just seems.This for fundamentally protect people healthy, preventing and curing diseases is of great immediate significance.
Anthocyanogen is stable compared with anthocyanidin active aglycone, but that is what to sacrifice the biological activity of anthocyanidin active aglycone be cost.Anthocyanogen and anthocyanidin are concepts as different as chalk and cheese.Even if anthocyanogen can enter blood of human body, but the glycosidic link that blood of human body (weakly alkaline) can not provide sour environment to make anthocyanogen is degraded and is obtained anthocyanidin, and the hydrionic activity hydroxy that can dissociate in blood of human body in anthocyanidin is non-existent in anthocyanogen.Anthocyanidin free in natural phant is seldom shown in.
Existing Industrialized processing technique, only has Chinese patent application, application number: 200910185829.2, the applying date: 2009.12.07, open (bulletin) day: 2010.06.30, open (bulletin) number: CN101760497A, Main classification number: C12P39/00 (2006.01) I, invention and created name: the microorganism of Anthocyanin is or/and biological enzyme extracting method is that anthocyanidin is the industrialization extractive technique of active aglycone.But because anthocyanidin biological activity is strong, chemical structure is unstable, only just stable under low temperature/acidity or drying conditions, under acid heating condition, easily decompose, and become water-insoluble substance, lose its biological activity, so above-mentioned patent application is carried out heat-sterilization to Biodegradation product (comprising microorganism, enzyme and anthocyanidin) by the temperature of 70 ℃-100 ℃, this will make the anthocyanidin of 10%-50% decompose sex change becomes water-insoluble substance and loses its biological activity, waste very valuable anthocyanidin Biological resources, increased anthocyanidin production cost.
Summary of the invention:
For effectively overcoming the defect of current existing anthocyanidin separation and extraction technology, fully and efficent use of resources, reduce production costs, the invention provides a kind of extracting method of more simple and reliable, technology is workable, production cost is lower, effective natural anthocyanidin active aglycone.Positively charged ion anthocyanidin active aglycone of the present invention, positively charged ion anthocyanidin, positively charged ion anthocyanidin aglycon, anthocyanidin active aglycone, anthocyanidin, its conceptual is identical.To the black rice starting material containing anthocyanidin, water directly soaks, and does not need to pulverize, to replace the first step of technical scheme.
The present invention utilizes the salt of positively charged ion anthocyanidin and anion surfactant under normal temperature and following temperature condition thereof, to be difficult to ionization in water and is water-insoluble throw out or this character of suspended substance, realizes the height of anthocyanidin and water and impurity wherein thereof and selects directional separation.Oleophilic group C chain in anion surfactant is longer, and its lipophilicity is stronger, wetting ability is poorer, easier and cationic substance (comprising positively charged ion anthocyanidin) chemical combination precipitation or suspension, and the temperature of water is lower simultaneously, and its precipitation or suspension effect are better; Precipitation or the suspension temperature of the salt of the anion surfactant that oleophilic group carbon (C) chain is shorter are lower, and precipitation or the suspension temperature of the salt of the anion surfactant that oleophilic group carbon (C) chain is longer are higher.
Diatomite is not hydrophilic, precipitates completely, and can absorb microorganism (enzyme) co-precipitation, but can not absorb water miscible anthocyanidin in the aqueous solution.The adsorbable water-insoluble material of microbial flocculant (comprising dead microorganism and inactivation biological enzyme), but can not adsorb water miscible anthocyanidin, its effect is identical with diatomite.
Obligate aerobic bacteria microorganism of the present invention is that [Xia Beicheng writes according to < < environmental pollutant biological degradation > > to the degraded of anion surfactant, Chemical Industry Press, Environmental Science and Engineering publishing centre publishes and distributes, March in 2002 the 1st edition, the 1st printing in March, 2002 Beijing, ISBN7-5025-3710-4/X147, No. 039, (Beijing) New Gordon word, No. 004930th, China depository library CIP data core word (2002), 186 pages, 179 pages, 54 pages, 43 pages, 42 pages, 35 pages, 34 pages], but bacterium does not produce polyphenoloxidase, the anion surfactant so it can only be degraded, and can not degrading phenol material positively charged ion anthocyanidin.Chinese patent application 200910185829.2 has been authorized.
Anion surfactant of the present invention can be described by the present invention obligate aerobic bacteria degradable; Hydrophilic group-SO in anion surfactant
4 2-with-COO
-can both by cytolemma, be absorbed degraded by aerobic bacteria; C element is degraded for bacterium provides necessary C source and energy; O element support aerobic bacteria free-dioxygen anaerobic respiration and be degraded; S element is converted into H in aerobic bacteria body
2s also excretes and is degraded, containing H
2after the aqueous solution of S is dried, H
2s gas is recovered; The straight-chain paraffin of C element between 10-22 and the phenyl ring being attached thereto are all easily degradable by aerobic bacteria of the present invention.
The technical solution adopted for the present invention to solve the technical problems is:
1, extracting method of the present invention comprises the following steps:
One, pre-treatment of raw material: the fresh starting material containing Anthocyanin, as Pericarpium Vitis viniferae, Radix Dauci Sativae, indigo fruit, classify → pulverize → eating soft water soaks the wall → heating of water suction → freezing destruction plant tissue cell and thaws → squeeze → filter standby (the 1) → residue of juice processed and suitably add water → soaking and stirring → squeezing → filtration juice processed standby (2);
Two, the above-mentioned squeeze and filter aqueous solution (1) and (2) are combined, and its water temperature are adjusted to the suitableeest scope of following degradation biological enzyme;
Three, above-mentioned product is carried out to biological degradation with the beta-glucosidase (beta-glycosidase) of cleavable anthocyanogen glycosidic link, first stirring at low speed, 50 revs/min-100 revs/min of rotating speeds, rear standing, make the glycosidic link in anthocyanogen be degraded and obtain positively charged ion anthocyanidin active aglycone; Cracking and degradation time 10 hours-24 hours, 25 ℃-50 ℃ of temperature, PH4.5-PH5;
Four, above-mentioned product is added again to the anion surfactant excessive a little with respect to positively charged ion anthocyanidin, as hexadecyl hydrosulfate ammonium (C
16h
33oSO
3nH
4), sodium stearyl sulfate, fatty acid sodium salt (RCOONa, R=C
12-C
22alkyl) or lipid acid sylvite, linear alkylbenzene sulphonic acid (C
10-C
22), the sodium salt of above-mentioned anion surfactant, sylvite or ammonium salt are identical in application function/effect of the present invention; With middling speed, stir, 600 revs/min-1400 revs/min of rotating speeds, its combination reaction temperature is 30 ℃-50 ℃, makes it fully reaction rear standing, then lowers the temperature, making aqueous temperature is 1 ℃-25 ℃, till making the most combined precipitation of positively charged ion anthocyanidin or suspension; This is conducive to quick and complete precipitation or the suspension of reactant;
Five, take out above-mentioned aquo compound, select obligate aerobic bacteria, in sealed vessel, input oxygen and carry out microbiological deterioration, can add a small amount of sterile water, in formerly standing after stirring at low speed, carry out, 30 revs/min of-50 revs/min of left and right of stirring velocity, make added anion surfactant be degraded by bacterial micro-organism, the cation activity of recovery anthocyanidin and water-soluble;
Biological degradation is carried out in the combination of one or more in concrete available section Bacillaceae, micrococcus sp, Nocardia, acinetobacter, brevibacterium sp, these microorganisms of Flavobacterium;
Degradation time 10 hours-24 hours, 20 ℃-30 ℃ of degradation temperatures, PH5-PH7; With organic acids such as citric acids, regulate pH value; The bacterial strain that does not produce water colo(u)r in Pseudomonas also can be used;
Six, above-mentioned aerobic bacteria Biodegradation product carried out to sterilization and remove bacterium and cellulase treatment, 1 ℃-30 ℃ of temperature, obtain positively charged ion anthocyanidin aqueous products:
(1). by above-mentioned degradation by bacteria product sealing, and stop supplies oxygen, 24 hours-72 hours hold-time; Or vacuumizing eliminating oxygen, pressure is not more than 25pa, 24 hours-72 hours hold-time; Make aerobic bacteria because lacking oxygen or/and lack the hydrophobic residual body of bacterium of the dead one-tenth of C nutrition source;
(2) need if desired to stir, make anthocyanidin fully water-soluble and separated with the residual body of bacterium, 300 revs/min-3000 revs/min of stirring velocitys, 10 minutes-60 minutes time, filter, remove the residual body of bacterium;
(3). with high-shear, stir, 1000 revs/min-3000 revs/min of rotating speeds, 30 minutes-60 minutes time, make the biological enzyme in the anthocyanidin aqueous solution lose activity and lose wetting ability;
(4). with ceramic membrane filter; Or with diatomite/microbial flocculant, in stirring, adsorb the residual body of deactivated biological enzyme, and standing, make the residual body Precipitation of diatomite/microbial flocculant and biological enzyme, then use the residual body of the residual biological enzyme of ceramic membrane filter; Thoroughly remove biological enzyme and the residual residual body of aerobic bacteria;
(5). obtain only containing the weakly acidic water solution of anthocyanidin, with food grade hydrochloric acid (HCL), regulate pH value, PH5-PH6.9; In above-mentioned (two), be with ceramic membrane or diatomite or microbial flocculant filtration;
Seven, above-mentioned the weakly acidic water solution containing anthocyanidin is carried out to vacuum lyophilization, and reclaim excessive HCL gas, temperature-40 ℃--1 ℃, pressure is not more than 25pa, 24 hours time; Obtain anthocyanidin active aglycone dry product.
2, described extracting method is preferably:
It is comprised of the following step:
One, pre-treatment of raw material: the fresh starting material containing Anthocyanin, as Pericarpium Vitis viniferae, Radix Dauci Sativae, indigo fruit, classify → pulverize → eating soft water soaks the wall → heating of water suction → freezing destruction plant tissue cell and thaws → squeeze → filter standby (the 1) → residue of juice processed and suitably add water → soaking and stirring → squeezing → filtration juice processed standby (2);
Two, the above-mentioned squeeze and filter aqueous solution (1) and (2) are combined, and its water temperature are adjusted to the suitableeest scope of following degradation biological enzyme;
Three, above-mentioned product is carried out to biological degradation with the beta-glucosidase (beta-glycosidase) of cleavable anthocyanogen glycosidic link, first stirring at low speed, 50 revs/min, rear standing, make the glycosidic link in anthocyanogen be degraded and obtain positively charged ion anthocyanidin active aglycone;
Cracking and degradation time 24 hours, temperature 50 C, PH5;
Four, above-mentioned product is added again to the anion surfactant hexadecyl hydrosulfate ammonium (C excessive a little with respect to positively charged ion anthocyanidin
16h
33oSO
3nH
4), with middling speed, stir, 600 revs/min, combination reaction temperature 50 C, makes it fully reaction rear standing, then lowers the temperature, and making aqueous temperature is 5 ℃, till making the most combined precipitation of positively charged ion anthocyanidin or suspension; This is conducive to quick and complete precipitation or the suspension of reactant;
Five, take out above-mentioned aquo compound, select obligate aerobic bacteria, carry out microbiological deterioration, can add a small amount of sterile water, in formerly standing after stirring at low speed, carry out, 30 revs/min of left and right of stirring velocity, are degraded by microorganisms added anion surfactant, the cation activity of recovery anthocyanidin and water-soluble;
Biological degradation is carried out in the combination of one or more in concrete available Nocardia, these microorganisms of acinetobacter;
Degradation time 24 hours, 25 ℃ of degradation temperatures, PH6; With citric acid, regulate pH value;
Six, above-mentioned bacterial micro-organism degraded product carried out to sterilization and remove bacterium and cellulase treatment, 1 ℃-30 ℃ of temperature, obtain positively charged ion anthocyanidin aqueous products:
(1). by above-mentioned degradation by bacteria product sealing, and residual oxygen in stop supplies oxygen and eliminating container, 72 hours hold-times; Make aerobic bacteria because lacking oxygen or/and lack the hydrophobic residual body of bacterium of the dead one-tenth of C nutrition source;
(2). need if desired to stir, make anthocyanidin fully water-soluble and separated with the residual body of bacterium, 3000 revs/min of stirring velocitys, 10 minutes time, with diatomite filtration, remove the residual body of bacterial micro-organism;
(3). with high-shear, stir, 3000 revs/min of rotating speeds, 30 minutes time, make the biological enzyme in the anthocyanidin aqueous solution lose activity and lose wetting ability;
(4). with ceramic membrane filter; Thoroughly remove biological enzyme and the residual residual body of aerobic bacteria;
(5). obtain only containing the weakly acidic water solution of anthocyanidin, with food grade hydrochloric acid (HCL), regulate pH value, PH6; Ceramic membrane is nonpolar mineral membrane, is easy to hydrophobic bacterium separated with anthocyanidin with enzyme;
Seven, above-mentioned the weakly acidic water solution containing anthocyanidin is carried out to vacuum lyophilization, and reclaim excessive HCL gas, temperature-40 ℃, pressure 15pa, 24 hours time; Obtain anthocyanidin active aglycone dry product.
The invention has the beneficial effects as follows:
1, the present invention has avoided the destruction to anthocyanidin active aglycone in sterilization production process to greatest extent, increase substantially the output capacity of anthocyanidin active aglycone, increased substantially the utilising efficiency of resource, starting material have been saved, stopped the discharge of anthocyanidin sex change waste, also save the energy, significantly reduced anthocyanidin production cost.This is very important and crucial technical requirements during anthocyanidin is produced.
2, the present invention can access real content at more than 95% positively charged ion anthocyanidin product, does not contain the product (may also contain a small amount of inabundant dry moisture) of bacterium anaerobic degradation.
3, bio-enzyme degradation product positively charged ion anthocyanidin of the present invention with anion surfactant combination reaction precipitate/suspension step and with the orderly combination that biological enzyme and bacterium step are removed in aerobic bacteria microbiological deterioration step and the sterilization of the compound of positively charged ion anthocyanidin and anion surfactant be that core technology of the present invention innovates essential features.
4, the present invention need carry out in without harmful microbe environment.
5, the selection of anion surfactant of the present invention requires:
(1) or low toxicity nontoxic to microorganism; (2) shorter (C of carbochain
10-C
22), containing straight chain oleophilic group; (3) biological degradability is good, and degraded relatively rapidly, thoroughly; (4) only containing C, H, O, S, N, P, these can be converted into the element of gaseous substance and water or maintain the necessary element of microbial life activity; (5) responsive to cationic substance or cationic chemical group;
Embodiment:
Below just embodiment the invention will be further described:
Embodiment:
During extraction, carry out as follows:
One, pre-treatment of raw material: the fresh starting material containing Anthocyanin, as Pericarpium Vitis viniferae, Radix Dauci Sativae, indigo fruit, classify → pulverize → eating soft water soaks the wall → heating of water suction → freezing destruction plant tissue cell and thaws → squeeze → filter standby (the 1) → residue of juice processed and suitably add water → soaking and stirring → squeezing → filtration juice processed standby (2);
Two, the above-mentioned squeeze and filter aqueous solution (1) and (2) are combined, and its water temperature are adjusted to the suitableeest scope of following degradation biological enzyme;
Three, above-mentioned product is carried out to biological degradation with the beta-glucosidase (beta-glycosidase) of cleavable anthocyanogen glycosidic link, first stirring at low speed, 50 revs/min, rear standing, make the glycosidic link in anthocyanogen be degraded and obtain positively charged ion anthocyanidin active aglycone; Cracking and degradation time 10 hours, temperature 50 C, PH4.5;
Four, above-mentioned product is added again to the anion surfactant fatty acid sodium salt excessive a little with respect to positively charged ion anthocyanidin (C16-C18 alkyl), with middling speed, stir, 1400 revs/min, combination reaction temperature 50 C, make it fully reaction rear standing, lower the temperature, making aqueous temperature is 25 ℃ again, till making the most combined precipitation of positively charged ion anthocyanidin or suspension; This is conducive to quick and complete precipitation or the suspension of reactant;
Five, take out above-mentioned aquo compound, select obligate aerobic bacteria, carry out microbiological deterioration, can add a small amount of sterile water, in formerly standing after stirring at low speed, carry out, 50 revs/min of left and right of stirring velocity, are degraded by microorganisms added anion surfactant, the cation activity of recovery anthocyanidin and water-soluble;
Biological degradation is carried out in the combination of one or more in concrete these microorganisms of available acinetobacter; Degradation time 24 hours, 30 ℃ of degradation temperatures, PH6.8; With citric acid, regulate pH value;
Six, above-mentioned bacterial micro-organism degraded product carried out to sterilization and remove bacterium and cellulase treatment, 1 ℃-30 ℃ of temperature, obtain positively charged ion anthocyanidin aqueous products:
(1). by above-mentioned degradation by bacteria product sealing, vacuumize eliminating oxygen, pressure 25pa, 48 hours hold-times; Make aerobic bacteria because lacking oxygen or/and lack the hydrophobic residual body of bacterium of the dead one-tenth of C nutrition source;
(2). need if desired to stir, make anthocyanidin fully water-soluble and separated with the residual body of bacterial micro-organism, 300 revs/min of stirring velocitys, 60 minutes time, with diatomite filtration, remove the residual body of bacterial micro-organism;
(3). with high-shear, stir, 2000 revs/min of rotating speeds, 60 minutes time, make the biological enzyme in the anthocyanidin aqueous solution lose activity and lose wetting ability;
(4). with diatomite, in stirring, adsorb the residual body of inactivation biological enzyme, standing, make the residual body Precipitation of diatomite and biological enzyme, then use the residual body of the residual biological enzyme of ceramic membrane filter; Thorough scavenger enzyme and bacterium;
(5). obtain only containing the weakly acidic water solution of anthocyanidin, with food grade hydrochloric acid (HCL), regulate pH value, PH6; In above-mentioned (two), (four), also can replace diatomite with microbial flocculant;
Seven, above-mentioned the weakly acidic water solution containing anthocyanidin is carried out to vacuum lyophilization, and reclaim excessive HCL gas, temperature-20 ℃, pressure 25pa, 24 hours time; Obtain anthocyanidin active aglycone dry product.
Claims (1)
1. the extracting method of natural anthocyanidin active aglycone, is characterized in that:
One, pre-treatment of raw material: the fresh starting material containing Anthocyanin, as Pericarpium Vitis viniferae, Radix Dauci Sativae, indigo fruit, classify → pulverize → eating soft water soaks the wall → heating of water suction → freezing destruction plant tissue cell and thaws → squeeze → filter standby (the 1) → residue of juice processed and suitably add water → soaking and stirring → squeezing → filtration juice processed standby (2);
Two, the above-mentioned squeeze and filter aqueous solution (1) and (2) are combined, and the temperature of its water are adjusted to the suitableeest scope of following the 3rd step degradation biological enzyme;
Three, by above-mentioned second step product, with the beta-glucosidase of cleavable anthocyanogen glycosidic link, be that beta-glycosidase carries out biological degradation, first stirring at low speed, 50 revs/min-100 revs/min, rear standing, make the glycosidic link in anthocyanogen be degraded and obtain positively charged ion anthocyanidin active aglycone; Cracking and degradation time 10 hours-24 hours, 25 ℃-50 ℃ of temperature, PH4.5-PH5;
Four, above-mentioned the 3rd step product is added again to the hexadecyl hydrosulfate ammonium (C excessive a little with respect to positively charged ion anthocyanidin
16h
33oSO
3nH
4) or sodium stearyl sulfate or fatty acid sodium salt or lipid acid sylvite or linear alkylbenzene sulphonic acid, the sodium salt of above-mentioned anion surfactant, sylvite or ammonium salt are identical in application function/effect of the present invention, with middling speed, stir, 600 revs/min-1400 revs/min, 30 ℃-50 ℃ of combination reaction temperature, make it fully reaction rear standing, then lower the temperature, making aqueous temperature is 1 ℃-25 ℃; Till making the most combined reaction precipitation of positively charged ion anthocyanidin or suspension; This is conducive to quick and complete precipitation or the suspension of reactant;
Five, take out above-mentioned the 4th step aquo compound, select obligate aerobic bacteria, carry out microbiological deterioration, can add a small amount of sterile water, in formerly standing after stirring at low speed, carry out, 30 revs/min-50 revs/min of stirring velocitys, make added anion surfactant be degraded by obligate aerobic bacteria; The cation activity of recovery anthocyanidin and water-soluble; Described obligate aerobic bacteria is one or more the combination in genus arthrobacter, micrococcus sp, Nocardia, acinetobacter, brevibacterium sp, these microorganisms of Flavobacterium;
Described obligate aerobic bacteria also comprises the bacterial strain that does not produce water colo(u)r in Pseudomonas;
Degradation time 10 hours-24 hours, 20 ℃-30 ℃ of degradation temperatures, PH5-PH7;
Six, above-mentioned the 5th step aerobic bacteria Biodegradation product carried out to sterilization and remove bacterium and cellulase treatment, 1 ℃-30 ℃ of temperature, obtain positively charged ion anthocyanidin aqueous products:
(1). by above-mentioned the 5th step aerobic bacteria Biodegradation product sealing, and stop supplies oxygen, 24 hours-72 hours hold-time; Or vacuumizing eliminating oxygen, pressure is not more than 25pa, 24 hours-72 hours hold-time; Make aerobic bacteria microorganism because lacking oxygen or/and lack the hydrophobic residual body of bacterium of the dead one-tenth of C nutrition source;
(2). need if desired to stir, make anthocyanidin fully water-soluble and separated with the residual body of aerobic bacteria microorganism, 300 revs/min-3000 revs/min of stirring velocitys, 10 minutes-60 minutes time, filter with diatomite/microbial flocculant; Remove the residual body of aerobic bacteria microorganism;
(3). with high-shear, stir 1000 revs/min-3000 revs/min of rotating speeds, 30 minutes-60 minutes time; Make the biological enzyme in the anthocyanidin aqueous solution lose activity and lose wetting ability;
(4). with ceramic membrane filter; Or with diatomite/microbial flocculant, in stirring, adsorb the residual body of deactivated biological enzyme, and standing, make the residual body Precipitation of diatomite/microbial flocculant and biological enzyme, then use the residual body of the residual biological enzyme of ceramic membrane filter; Thoroughly remove biological enzyme and the residual residual body of aerobic bacteria;
(5). obtain only containing the weakly acidic water solution of anthocyanidin, with food grade hydrochloric acid (HCL), regulate pH value, PH5-PH6.9; In above-mentioned (two), can also use ceramic membrane filter;
Seven, above-mentioned the 6th step is only carried out to vacuum lyophilization containing the weakly acidic water solution of anthocyanidin, and reclaim excessive HCL gas, temperature-40 ℃--1 ℃, pressure is not more than 25pa, 24 hours time; Obtain anthocyanidin active aglycone dry product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310112792.7A CN104059951A (en) | 2013-03-18 | 2013-03-18 | Extraction method of natural anthocyanidin active aglycone |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310112792.7A CN104059951A (en) | 2013-03-18 | 2013-03-18 | Extraction method of natural anthocyanidin active aglycone |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104059951A true CN104059951A (en) | 2014-09-24 |
Family
ID=51547882
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310112792.7A Pending CN104059951A (en) | 2013-03-18 | 2013-03-18 | Extraction method of natural anthocyanidin active aglycone |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104059951A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105219122A (en) * | 2015-09-11 | 2016-01-06 | 张家港天隆针织服饰织造有限公司 | A kind of indigo fruit prepares the method for vegetable dye |
| WO2018205846A1 (en) * | 2017-05-11 | 2018-11-15 | 耿兆翔 | Method for extracting highly-effective natural plant anthocyanidin |
| CN110372770A (en) * | 2018-04-12 | 2019-10-25 | 耿兆翔 | The highly effective extraction method of natural hydroxyl or/and carboxyl compound |
| CN111041053A (en) * | 2018-10-15 | 2020-04-21 | 耿兆翔 | Efficient extraction method of plant anthocyanin |
| CN111139274A (en) * | 2018-11-02 | 2020-05-12 | 耿兆翔 | Method for extracting plant anthocyanin active aglycone |
| CN114031591A (en) * | 2021-12-02 | 2022-02-11 | 黑龙江邦超生物科技有限公司 | Vacuum freezing solvent extraction method of anthocyanin |
-
2013
- 2013-03-18 CN CN201310112792.7A patent/CN104059951A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105219122A (en) * | 2015-09-11 | 2016-01-06 | 张家港天隆针织服饰织造有限公司 | A kind of indigo fruit prepares the method for vegetable dye |
| WO2018205846A1 (en) * | 2017-05-11 | 2018-11-15 | 耿兆翔 | Method for extracting highly-effective natural plant anthocyanidin |
| CN108864027A (en) * | 2017-05-11 | 2018-11-23 | 耿兆翔 | The extracting method of efficient natural Anthocyanin |
| CN110372770A (en) * | 2018-04-12 | 2019-10-25 | 耿兆翔 | The highly effective extraction method of natural hydroxyl or/and carboxyl compound |
| CN111041053A (en) * | 2018-10-15 | 2020-04-21 | 耿兆翔 | Efficient extraction method of plant anthocyanin |
| CN111139274A (en) * | 2018-11-02 | 2020-05-12 | 耿兆翔 | Method for extracting plant anthocyanin active aglycone |
| CN114031591A (en) * | 2021-12-02 | 2022-02-11 | 黑龙江邦超生物科技有限公司 | Vacuum freezing solvent extraction method of anthocyanin |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101760497B (en) | Microorganisms or/and biological enzyme extracting method for plant anthocyanin | |
| CN104059951A (en) | Extraction method of natural anthocyanidin active aglycone | |
| CN101144097B (en) | Method for preparing chitin and its chitosan and chitosan oligosaccharide | |
| CN103478439B (en) | Shrimp feed addictive composition and manufacture method thereof | |
| CN106479927A (en) | Method and its application using Bacillus licheniformis biosynthesiss nanometer selenium | |
| CN105779356B (en) | A kind of microorganism self-assembled nanometer material and its preparation method and application | |
| CN102703342B (en) | Bacillus velezensis ZJ20 strain and liquid preparations thereof | |
| CN104892785A (en) | Extraction method of seaweed polysaccharide | |
| CN106472962A (en) | The detoxifying agent of 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone and its preparation technology in a kind of biodegradation feedstuff | |
| CN102899269B (en) | Cypermethrin degrading bacteria in marine environment, and separation, purification and application thereof | |
| CN110495611A (en) | A kind of technique improving sea cucumber nutritional health effect | |
| CN110301601A (en) | A kind of soluble Kelp Powder and its Production by Enzymes method containing brown alga oligose | |
| CN106472961A (en) | The detoxifying agent of ochratoxin and its preparation technology in a kind of biodegradation feedstuff | |
| CN103834702A (en) | Extraction method of cationic anthocyanidin aglycone | |
| CN112011531A (en) | Enzyme preparation produced by using immobilized cell technology and preparation method thereof | |
| CN102586378A (en) | Method for extracting substances from fermented shrimp head shells | |
| CN104357332A (en) | Aspergillus niger JH-2 and application to biotransformation and synthesis of asiatic acid | |
| CN102168122B (en) | A method employing Enterococcus faecalis fermentation for preparing chitin from exoskeletons of crustaceans | |
| CN101891307A (en) | Marine culture biological cleanser and preparation method thereof | |
| CN110964674A (en) | Photosynthetic bacteria for high yield of nano-selenium and preparation method of nano-selenium viable bacteria preparation thereof | |
| CN107201382B (en) | A kind of detergent and its preparation method and application | |
| CN105087427A (en) | Vibrio natriegens for producing agarase and application of vibrio natriegens | |
| CN101139569B (en) | alpha-amylase inhibitor production bacterium and method for preparing alpha-amylase inhibitor and application | |
| CN109053293A (en) | A kind of purification soil fertilizer and its application method | |
| CN103892126B (en) | Modifying agent raised by livestock and poultry colony house |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140924 |