CN104041597B - Preparation method for formula milk powder with high oxidization resistance - Google Patents

Abstract

The invention discloses a preparation method for formula milk powder with high oxidization resistance and belongs to the technical field of processing of dairy products. The preparation method comprises the following steps: carrying out enzymolysis on goat cheese protein by using pepsase and trypsin in sequence to prepare goat cheese protein double enzymatic hydrolysate; and adding the goat cheese protein double enzymatic hydrolysate into a previous formula to obtain the formula milk powder with the high oxidization resistance. According to the milk powder prepared by the invention, the removing rate of hydroxyl radicals is obviously improved when being compared with that of the formula milk powder added with single enzymatic hydrolysate. When the adding amount of the goat cheese protein double enzymatic hydrolysate is 1.10mg/100g, the removing rate of the hydroxyl radicals of the prepared formula milk powder can be up to 86.24%.

Description

A kind of preparation method of high antioxidant formula milk

Technical field

The present invention relates to the preparation method of a kind of high antioxidant formula milk, belong to technical field of processing dairy products.

Background technology

Lac caprae seu ovis contains less casein, more alpha lactalbumin relative to Lac Bovis seu Bubali, and the grumeleuse that its protein is formed under one's belt is more Carefully, it is easier to digest and assimilate；The Oil globule of Lac caprae seu ovis is the 1/3 of Lac Bovis seu Bubali, and spreads uniformly, is easily absorbed by intestinal wall；Lac caprae seu ovis There is more insatiable hunger and fatty acid, it is easier to the chyle in small intestinal absorbs；Do not contain only abundant aminoacid ingredient, Er Qieyou The phosphorus of high-load, cobalt and vitamin A, B1, C and D.Lac caprae seu ovis have nutritious, be prone to absorption, antiallergic, useful strong The features such as health, are suitable to most crowd and eat.Lac caprae seu ovis can increase the disease-resistant ability of human body, containing as human milk in Lac caprae seu ovis Active factors epithelial cell growth factor (EGF), promotes the growth of epithelial cell, strengthens the disease-resistant ability of human body, and therefore Lac caprae seu ovis is mesh The front milk closest to human milk generally acknowledged both at home and abroad.

Casein, as protein component important in Lac caprae seu ovis, mainly has α s1-CN, α s2-CN, β-CN and four kinds of Asias of κ-CN Type, the function of biologically active peptide produced currently, with respect to casein enzymolysis has a lot of report, mainly thinks Casein in Milk bioactive peptide There is promotion body alimentation, ACE inhibitory action, opioid activity, antioxidant activity, ACE inhibitory activity and immunity adjust Joint effect.

The aging of body is mainly due to Oxidation, and along with the raising of people's living standard, people were more desirable to healthy, full The life of vigor, antioxidation currently suffers from the concern of masses, especially women and pays close attention to oxidation resistant product, it is seen that antioxidation Product has market prospect widely.

Along with the raising of quality of life, people are more and more higher to the requirement of quality of the life, and food is only the most no longer Meet requirement of having enough to eat and wear, it is desirable to food and have higher quality, meet the more preferable pleasure of the senses of people and more abundant nutrition Demand, people want to be obtained in that more wholesome function while dietary intake, the most substantial amounts of health food Frequently occur in face of masses, be also deeply loved by the public.But the various food quality problems recently occurred, make " food safety " This noun occurs in the visual field of masses, producing mainly due to for reaching the demand of certain sense organ or function of food-safety problem Some composition added artificially in food so that it is there is certain specific function or there is good organoleptic quality, due to greatly Most adding ingredients is all the non-natural prods of synthetic, brings various clear and definite and indefinite danger may to the health of human body Evil, consequently found that a kind of functional food adding natural component all has immeasurable meaning to food industry or even the mankind, This research is with natural component sheep casein as raw material, after enzyme hydrolysis, measures its antioxidant properties, successfully invents one The formula milk of high antioxidant.

Summary of the invention

The invention provides the preparation method of a kind of high antioxidant formula milk, the technical scheme taked is as follows:

A kind of preparation method of high antioxidant formula milk, step is as follows:

1) by after feta protein dissolution, first use pepsin enzymolysis, obtain pepsin hydrolysis products after enzyme denaturing, recycle Trypsin Enzyme enzymolysis further to enzymatic hydrolysate, utilizes ultrafiltration separation enzymatic hydrolysate after enzyme denaturing, collect filtrate, carry out de-bitterness, decolouring, Degassing processes, and obtains feta albumen double enzymolysis product after spray drying；

2) in milk powder, feta albumen double enzymolysis product is added.

In described method, the addition of feta albumen double enzymolysis product is 1.0～1.2mg/100g, the addition of preferred enzyme hydrolysis products For 1.10mg/100g.

In described method, pepsic enzyme is lived as 2400U/mg.

In described method, pepsin hydrolysis temperature is 37 DEG C, enzymolysis time 90min.

In described method, tryptic enzyme is lived as 120U/mg.

In described method, trypsin digestion temperature is 37 DEG C, enzymolysis time 90min.

Specifically comprising the following steps that of described method

1) by after feta protein dissolution, being first 2400U/mg pepsin enzymolysis 90min with enzyme work, hydrolysis temperature is 37 DEG C, It is 120U/mg trypsin enzymolysis further to enzymatic hydrolysate that recycling enzyme is lived, and hydrolysis temperature is 37 DEG C, and enzymolysis time is 90min, utilizes ultrafiltration separation enzymatic hydrolysate after enzyme denaturing, collect filtrate, carry out de-bitterness, decolour, degassing processes, spray dried Feta albumen double enzymolysis product is obtained after dry；

2) in milk powder, feta albumen double enzymolysis product is added by the addition of 1.0～1.2mg/100g.

The formula of the present invention is:

Every 100g formula milk contains: fresh milk, desalted whey powder, lactose, refining vegetable oil >=17.0mg, alpha-lactalbumin 1200mg, AA (arachidonic acid) 80mg, DHA (docosahexenoic acid) 50mg, nucleotide (5 '-monophosphate born of the same parents Glycosides, 5 '-Uridylic acid disodium salt, 5 '-Guanosine 5'-Monophosphate disodium, 5 '-AMP) >=40mg, taurine >=35mg, choline >=40mg, VBT >=4mg, beta-carotene >=80 μ g, vitamin A, 2000IU, vitamin D3 380IU, vitamin C40mg, vitamin B1 >=500 μ g, vitamin B2 >=600 μ g, vitamin B6 >=300 μ g, vitamin B12 >=1.2 μ g, Vitamin E >=8IU, vitamin K1 >=30 μ g, nicotinic acid >=420 μ g, folic acid >=40 μ g, pantothenic acid >=2000 μ g, biotin >=9 μ g, Calcium lactate >=4mg, calcium hydrogen phosphate >=4mg, ferrous lactate 8mg, zinc lactate 4mg, manganese sulfate >=30 μ g, potassium iodate≤1000mg, With the addition of again the sheep casein double enzymolysis product of 1.10mg.

Beneficial effect: the present invention is first feta albumen to carry out single, double enzymolysis under in vitro conditions respectively, obtains corresponding enzymolysis Product, finds that this enzymatic hydrolysate has antioxidant activity, reaches 70.76% through pepsin enzymolysis afterproduct non-oxidizability, pancreas egg White enzyme enzymolysis afterproduct non-oxidizability reaches 75.67%, and pepsin and trypsin double enzymolysis afterproduct non-oxidizability reach 86.50%, find that double enzymolysis afterproduct non-oxidizability significantly improves, and double enzymolysis product addition is when 1.10mg/100g, Its non-oxidizability tends to the highest, therefore adds this double enzymolysis product in milk powder with the addition of 1.10mg/100g, in human body High anti-oxidation function can be produced, thus human body is produced beneficial effect.

Accompanying drawing explanation

Fig. 1 is pepsin and trypsin but double enzymolysis sheep casein hydrolysates non-oxidizability testing result；

(1: pepsin enzymolysis sheep casein product；2: trypsin digestion sheep casein product；3: pepsin and trypsin Double enzymolysis sheep casein product).

Fig. 2 is the non-oxidizability of the double enzymolysis product formulation milk powder adding different content.

Detailed description of the invention

The present invention obtains the caseic enzymatic hydrolysate of Lac caprae seu ovis by pepsin caseic to Lac caprae seu ovis and tryptic double enzymolysis, And add to this product existing formula milk obtains there is the formula milk of high antioxidant.Prepared milk powder has high anti- Oxidability.Below in conjunction with embodiment, the present invention will be further described, but the present invention should not be limited by the examples.

Material and reagent:

Lac caprae seu ovis, picks up from Suiling County Fu Wang milk goat plant；Pepsin (enzyme 2400U/mg alive), trypsin enzyme alive 120 U/mg) Sigma company；1.10-phenanthrene quinoline (orthophenanthroline), Lay biology company limited of NaOH U.S.；Acetic acid, sodium acetate FeSO4,30% hydrogen peroxide, NaH₂PO₄、Na₂HPO₄, Ke Miou chemical reagent development centre, Tianjin.

Ultrafiltration cup, ultraviolet spectrophotometer UV-2450, electronic thermostatic water-bath, vortex oscillator, electronic analytical balance, pH Acidometer, spray dryer.

Embodiment 1: the caseic preparation of sheep

Take the fresh Lac caprae seu ovis of 1000mL through gauze filtering and impurity removing matter.4 DEG C of 2000g/min are centrifuged 10min defat.Use 2mol/L HCl Solution regulation pH value, to casein isoelectric point, IP 4.6, static 30min, 1000g/min, centrifugal 10min, abandons supernatant, precipitation With the Acetic acid-sodium acetate buffer solution 2 times of pH4.6,1000g/min is centrifuged 5min, and gained precipitate is casein Crude product, recording its quality is 230.4mg.

Embodiment 2: the caseic single enzymolysis of sheep

Weigh the sheep casein crude product 10mg of preparation, help it to dissolve with 0.1mol/L NaOH, be settled to 100 with distilled water ML, regulation pH to pepsin optimum pH 3.0, add the enzyme pepsin for 2400U alive, carry out in 37 DEG C of water-baths Enzymolysis, after enzymolysis 90min, is placed in 10min in boiling water and makes enzyme inactivate, and enzymolysis reaction, 1000g/min is centrifuged 30min Remove unhydrolysed casein, with the ultrafiltration cup of the molecular cut off of 6ku, enzymatic hydrolysate is carried out ultrafiltration, collect filtrate, to it Carry out de-bitter, decolour, after degassing processes, be spray-dried, obtain anti-oxidation peptide dry powder.It is placed in 4 DEG C of refrigerators, in case surveying Fixed.

Embodiment 3: the caseic single enzymolysis of sheep

Weighing the sheep casein crude product 10mg of preparation, help it to dissolve with 0.1mol/L NaOH, distilled water is settled to 100mL, Regulation pH, to trypsin optimum pH 8.0, adds the enzyme trypsin for 120U alive, carries out enzymolysis in 37 DEG C of water-baths, After enzymolysis 90min, it is placed in 10min in boiling water and makes enzyme inactivate, with enzymolysis reaction, be centrifuged 30min with 1000g/min Remove unhydrolysed casein, with the ultrafiltration cup of the molecular cut off of 6ku, enzymatic hydrolysate is carried out ultrafiltration, collect filtrate, to it Carry out de-bitter, decolour, after degassing processes, be spray-dried, obtain anti-oxidation peptide dry powder.It is placed in 4 DEG C of refrigerators, in case surveying Fixed.

Embodiment 4: the caseic pepsin of sheep and tryptic double enzymolysis

Take 10mg sheep casein crude product to be dissolved in 10mL ultra-pure water, at 37 DEG C, add the pepsin of enzyme 2400U alive, Reactant liquor is placed on shaking table, is placed in 37 DEG C of water-baths and carries out enzymolysis, after enzymolysis 90min, be placed in 10min in boiling water and make enzyme Inactivation, with enzymolysis reaction.At 37 DEG C, add the trypsin of enzyme 120U alive, reactant liquor is placed on shaking table, is placed in 37 DEG C of water-baths carry out enzymolysis, after enzymolysis 90min, is placed in 10min in boiling water and makes enzyme inactivate, with enzymolysis reaction.With 6 The ultrafiltration cup of the molecular cut off of ku carries out ultrafiltration to enzymatic hydrolysate, collects filtrate, to its carry out de-bitter, decolour, degassing processes After, it is spray-dried, obtains anti-oxidation peptide dry powder.It is placed in 4 DEG C of refrigerators, in case measuring.

Embodiment 5: orthophenanthroline-Fe²⁺Method measures the non-oxidizability of yak milk casein enzymatic hydrolysate

Take above-mentioned prepared pepsin enzymolysis sheep caseic enzymatic hydrolysate 1mg, be dissolved in 1mL distilled water, be saved in 4 DEG C Under the conditions of standby.

(1) being sequentially added into 1mL orthophenanthroline in 25mL test tube with ground stopper, 2mL pH7.4PBS buffer, 1mL steams Distilled water, fully shakes up, and adds 1mL FeSO₄The 5s that vibrates on solution, with vortex oscillator makes it fully mix, and adds 1mL H₂O₂, tool plug, in 37 DEG C of reactions, reaction measures rapidly its absorbance after terminating at wavelength 536nm, and its measured value is A_p。

(2) 1mL H in (1) is replaced with 1mL distilled water₂O₂, surveying its absorbance is A_b。

(3) replace, with 1mL pepsin enzymolysis sheep caseic enzymatic hydrolysate solution, the distilled water that in (1), the 1st time adds.Survey Its absorbance fixed is A_S。

Hydroxy radical (HO) clearance rate (D) is calculated as follows:

$D/(\%)=\frac{\mathrm{As}-\mathrm{Ap}}{\mathrm{Ab}-\mathrm{Ap}}\×100$

Result is as it is shown in figure 1, sheep casein reaches 70.76% through pepsin enzymolysis afterproduct non-oxidizability.

Embodiment 6: orthophenanthroline-Fe²⁺Method measures the non-oxidizability of yak milk casein enzymatic hydrolysate

Take above-mentioned prepared trypsin digestion sheep caseic enzymatic hydrolysate 1mg, be dissolved in 1mL distilled water, be saved in 4 DEG C Under the conditions of standby.

(1) being sequentially added into 1mL orthophenanthroline in 25mL test tube with ground stopper, 2mL pH7.4PBS buffer, 1mL steams Distilled water, fully shakes up, and adds 1mL FeSO₄The 5s that vibrates on solution, with vortex oscillator makes it fully mix, and adds 1mL H₂O₂, tool plug, in 37 DEG C of reactions, reaction measures rapidly its absorbance after terminating at wavelength 536nm, and its measured value is A_p。

(2) 1mL H in (1) is replaced with 1mL distilled water₂O₂, surveying its absorbance is A_b。

(3) replace, with the 1mL caseic enzymatic hydrolysate of trypsin digestion sheep, the distilled water that in solution (1), the 1st time adds. Measuring its absorbance is A_S。

Hydroxy radical (HO) clearance rate (D) is calculated as follows:

$D/(\%)=\frac{\mathrm{As}-\mathrm{Ap}}{\mathrm{Ab}-\mathrm{Ap}}\×100$

Result is as it is shown in figure 1, the non-oxidizability of the caseic enzymatic hydrolysate of trypsin digestion sheep reaches 75.67%.

Embodiment 7: orthophenanthroline-Fe²⁺Method measures the non-oxidizability of yak milk casein enzymatic hydrolysate

Take above-mentioned prepared pepsin and trypsin double enzymolysis sheep caseic enzymatic hydrolysate 1mg, be dissolved in 1mL distilled water In, standby under the conditions of being saved in 4 DEG C.

(1) being sequentially added into 1mL orthophenanthroline in 25mL test tube with ground stopper, 2mL pH7.4PBS buffer, 1mL steams Distilled water, fully shakes up, and adds 1mL FeSO₄The 5s that vibrates on solution, with vortex oscillator makes it fully mix, and adds 1mL H₂O₂, tool plug, in 37 DEG C of reactions, reaction measures rapidly its absorbance after terminating at wavelength 536nm, and its measured value is A_p。

(2) 1mL H in (1) is replaced with 1mL distilled water₂O₂, surveying its absorbance is A_b。

(3) replace (1) adds for the 1st time with 1mL pepsin and trypsin double enzymolysis sheep caseic enzymatic hydrolysate solution The distilled water entered.Measuring its absorbance is A_S。

Hydroxy radical (HO) clearance rate (D) is calculated as follows:

$D/(\%)=\frac{\mathrm{As}-\mathrm{Ap}}{\mathrm{Ab}-\mathrm{Ap}}\×100$

Result is as it is shown in figure 1, the non-oxidizability of pepsin and the caseic enzymatic hydrolysate of trypsin double enzymolysis sheep reaches 86.50%, it is significantly higher than pepsin and the non-oxidizability (P ＜ 0.05) of the trypsin list caseic enzymatic hydrolysate of enzymolysis sheep. Embodiment 8: orthophenanthroline-Fe²⁺Method measures the non-oxidizability of the formula milk adding sheep casein hydrolysates.

A kind of formula milk, every 100g formula milk contains: fresh milk, desalted whey powder, lactose, refining vegetable oil >=17.0mg, Alpha-lactalbumin 1200g, AA (arachidonic acid) 80mg, DHA (docosahexenoic acid) 50mg, nucleotide (5 '- Monophosphate cytidine, 5 '-Uridylic acid disodium salt, 5 '-Guanosine 5'-Monophosphate disodium, 5 '-AMP) >=40mg, taurine >=35mg, Choline >=40mg, VBT >=4mg, beta-carotene >=80 μ g, vitamin A, 2000IU, vitamin D3 380IU, dimension Raw element C40mg, vitamin B1 >=500 μ g, vitamin B2 >=600 μ g, vitamin B6 >=300 μ g, vitamin B12 >=1.2 μ g, Vitamin E >=8IU, vitamin K1 >=30 μ g, nicotinic acid >=420 μ g, folic acid >=40 μ g, pantothenic acid >=2000 μ g, biotin >=9 μ g, Calcium lactate >=4mg, calcium hydrogen phosphate >=4mg, ferrous lactate 8mg, zinc lactate 4mg, manganese sulfate >=30 μ g, potassium iodate≤1000mg, With the addition of again the sheep casein double enzymolysis product of 1.10mg.

Take above-mentioned prepared formula milk 10mg, be dissolved in 10mL distilled water, standby under the conditions of being saved in 4 DEG C.

(1) being sequentially added into 1mL orthophenanthroline in 25mL test tube with ground stopper, 2mL pH7.4PBS buffer, 1mL steams Distilled water, fully shakes up, and adds 1mL FeSO₄The 5s that vibrates on solution, with vortex oscillator makes it fully mix, and adds 1mL H₂O₂, tool plug, in 37 DEG C of reactions, reaction measures rapidly its absorbance after terminating at wavelength 536nm, and its measured value is A_p。

(2) 1mL H in (1) is replaced with 1mL distilled water₂O₂, surveying its absorbance is A_b。

(3) replace, with 1mL formula milk solution, the distilled water that in (1), the 1st time adds.Measuring its absorbance is A_S。

Hydroxy radical (HO) clearance rate (D) is calculated as follows:

$D/(\%)=\frac{\mathrm{As}-\mathrm{Ap}}{\mathrm{Ab}-\mathrm{Ap}}\×100$

Result is as in figure 2 it is shown, pepsin and trypsin double enzymolysis sheep caseic enzymatic hydrolysate addition are 1.00mg/100g Time, its non-oxidizability reaches 84.59%.

Embodiment 9: orthophenanthroline-Fe²⁺Method measures the non-oxidizability of the formula milk adding sheep casein hydrolysates

A kind of formula milk, every 100g formula milk contains: fresh milk, desalted whey powder, lactose, refining vegetable oil >=17.0mg, Alpha-lactalbumin 1200g, AA (arachidonic acid) 80mg, DHA (docosahexenoic acid) 50mg, nucleotide (5 '- Monophosphate cytidine, 5 '-Uridylic acid disodium salt, 5 '-Guanosine 5'-Monophosphate disodium, 5 '-AMP) >=40mg, taurine >=35mg, Choline >=40mg, VBT >=4mg, beta-carotene >=80 μ g, vitamin A, 2000IU, vitamin D3 380IU, dimension Raw element C40mg, vitamin B1 >=500 μ g, vitamin B2 >=600 μ g, vitamin B6 >=300 μ g, vitamin B12 >=1.2 μ g, Vitamin E >=8IU, vitamin K1 >=30 μ g, nicotinic acid >=420 μ g, folic acid >=40 μ g, pantothenic acid >=2000 μ g, biotin >=9 μ g, Calcium lactate >=4mg, calcium hydrogen phosphate >=4mg, ferrous lactate 8mg, zinc lactate 4mg, manganese sulfate >=30 μ g, potassium iodate≤1000mg, With the addition of again the sheep casein double enzymolysis product of 1.10mg.

Take above-mentioned prepared formula milk 10mg, be dissolved in 10mL distilled water, standby under the conditions of being saved in 4 DEG C.

(1) being sequentially added into 1mL orthophenanthroline in 25mL test tube with ground stopper, 2mL pH7.4PBS buffer, 1mL steams Distilled water, fully shakes up, and adds 1mL FeSO₄The 5s that vibrates on solution, with vortex oscillator makes it fully mix, and adds 1mL H₂O₂, tool plug, in 37 DEG C of reactions, reaction measures rapidly its absorbance after terminating at wavelength 536nm, and its measured value is A_p。

(2) 1mL H in (1) is replaced with 1mL distilled water₂O₂, surveying its absorbance is A_b。

(3) replace, with 1mL formula milk solution, the distilled water that in (1), the 1st time adds.Measuring its absorbance is A_S。

Hydroxy radical (HO) clearance rate (D) is calculated as follows:

$D/(\%)=\frac{\mathrm{As}-\mathrm{Ap}}{\mathrm{Ab}-\mathrm{Ap}}\×100$

Result is as in figure 2 it is shown, pepsin and trypsin double enzymolysis sheep caseic enzymatic hydrolysate addition are 1.10mg/100g Time, its non-oxidizability is significantly higher than other two experimental grouies and reaches 86.24% (P ＜ 0.05).

Embodiment 10: orthophenanthroline-Fe²⁺Method measures the non-oxidizability of the formula milk adding sheep casein hydrolysates

A kind of formula milk, every 100g formula milk contains: fresh milk, desalted whey powder, lactose, refining vegetable oil >=17.0mg, Alpha-lactalbumin 1200g, AA (arachidonic acid) 80mg, DHA (docosahexenoic acid) 50mg, nucleotide (5 '- Monophosphate cytidine, 5 '-Uridylic acid disodium salt, 5 '-Guanosine 5'-Monophosphate disodium, 5 '-AMP) >=40mg, taurine >=35mg, Choline >=40mg, VBT >=4mg, beta-carotene >=80 μ g, vitamin A, 2000IU, vitamin D3 380IU, dimension Raw element C40mg, vitamin B1 >=500 μ g, vitamin B2 >=600 μ g, vitamin B6 >=300 μ g, vitamin B12 >=1.2 μ g, Vitamin E >=8IU, vitamin K1 >=30 μ g, nicotinic acid >=420 μ g, folic acid >=40 μ g, pantothenic acid >=2000 μ g, biotin >=9 μ g, Calcium lactate >=4mg, calcium hydrogen phosphate >=4mg, ferrous lactate 8mg, zinc lactate 4mg, manganese sulfate >=30 μ g, potassium iodate≤1000mg, With the addition of again the sheep casein double enzymolysis product of 1.10mg.

Take above-mentioned prepared formula milk 10mg, be dissolved in 10mL distilled water, standby under the conditions of being saved in 4 DEG C.

(1) being sequentially added into 1mL orthophenanthroline in 25mL test tube with ground stopper, 2mL pH7.4PBS buffer, 1mL steams Distilled water, fully shakes up, and adds 1mL FeSO₄The 5s that vibrates on solution, with vortex oscillator makes it fully mix, and adds 1mL H₂O₂, tool plug, in 37 DEG C of reactions, reaction measures rapidly its absorbance after terminating at wavelength 536nm, and its measured value is A_p。

(2) 1mL H in (1) is replaced with 1mL distilled water₂O₂, surveying its absorbance is A_b。

(3) replace, with 1mL formula milk solution, the distilled water that in (1), the 1st time adds.Measuring its absorbance is A_S。

Hydroxy radical (HO) clearance rate (D) is calculated as follows:

$D/(\%)=\frac{\mathrm{As}-\mathrm{Ap}}{\mathrm{Ab}-\mathrm{Ap}}\×100$

Result is as in figure 2 it is shown, pepsin and trypsin double enzymolysis sheep caseic enzymatic hydrolysate addition are 1.20mg/100g Time, its non-oxidizability reaches 85.02%.

Claims (6)

1. the preparation method of a high antioxidant formula milk, it is characterised in that step is as follows:

1) by after feta protein dissolution, first use pepsin enzymolysis, obtain pepsin hydrolysis products after enzyme denaturing, recycle Trypsin Enzyme enzymolysis further to enzymatic hydrolysate, utilizes ultrafiltration separation enzymatic hydrolysate after enzyme denaturing, collect filtrate, carries out de-bitterness, takes off Color, degassing process, and obtain feta albumen double enzymolysis product after spray drying；

2) adding feta albumen double enzymolysis product in milk powder, the addition of feta albumen double enzymolysis product is 1.0～1.2 mg/100g。

2. method described in claim 1, it is characterised in that described pepsic enzyme is lived as 2400U/mg.

3. method described in claim 1, it is characterised in that pepsin hydrolysis temperature is 37 DEG C, enzymolysis time 90min.

4. method described in claim 1, it is characterised in that described tryptic enzyme is lived as 120U/mg.

5. method described in claim 1, it is characterised in that trypsin digestion temperature is 37 DEG C, enzymolysis time 90min.

6. method described in claim 1, it is characterised in that specifically comprise the following steps that

1) by after feta protein dissolution, being first 2400U/mg pepsin enzymolysis 90min with enzyme work, hydrolysis temperature is 37 DEG C, It is 120U/mg trypsin enzymolysis further to enzymatic hydrolysate that recycling enzyme is lived, and hydrolysis temperature is 37 DEG C, and enzymolysis time is 90min, utilizes ultrafiltration separation enzymatic hydrolysate after enzyme denaturing, collect filtrate, carry out de-bitterness, decolour, degassing processes, spray Mist is dried obtains feta albumen double enzymolysis product；

2) in milk powder, feta albumen double enzymolysis product is added by the addition of 1.0～1.2mg/100g.