CN104039304A - Oral bacteria proliferation inhibitor - Google Patents
Oral bacteria proliferation inhibitor Download PDFInfo
- Publication number
- CN104039304A CN104039304A CN201280032036.7A CN201280032036A CN104039304A CN 104039304 A CN104039304 A CN 104039304A CN 201280032036 A CN201280032036 A CN 201280032036A CN 104039304 A CN104039304 A CN 104039304A
- Authority
- CN
- China
- Prior art keywords
- sorbitol
- galactose
- oral cavity
- culture medium
- bacterium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000894006 Bacteria Species 0.000 title claims abstract description 38
- 230000035755 proliferation Effects 0.000 title abstract description 13
- 239000003112 inhibitor Substances 0.000 title abstract 3
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims abstract description 41
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims abstract description 40
- 239000000600 sorbitol Substances 0.000 claims abstract description 38
- 229930182830 galactose Natural products 0.000 claims abstract description 37
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 claims abstract description 16
- 210000000214 mouth Anatomy 0.000 claims description 29
- 241000605986 Fusobacterium nucleatum Species 0.000 claims description 23
- 230000002634 anti-blastic effect Effects 0.000 claims description 13
- 230000003239 periodontal effect Effects 0.000 claims description 10
- 244000052769 pathogen Species 0.000 claims description 8
- 230000001717 pathogenic effect Effects 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 5
- 235000013305 food Nutrition 0.000 claims description 2
- 208000028169 periodontal disease Diseases 0.000 abstract description 11
- 230000002265 prevention Effects 0.000 abstract description 6
- 150000001720 carbohydrates Chemical class 0.000 abstract description 5
- 150000005846 sugar alcohols Chemical class 0.000 abstract description 3
- 230000037406 food intake Effects 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 229960002920 sorbitol Drugs 0.000 description 36
- 239000001963 growth medium Substances 0.000 description 25
- 239000001974 tryptic soy broth Substances 0.000 description 15
- 238000004519 manufacturing process Methods 0.000 description 10
- 206010006326 Breath odour Diseases 0.000 description 7
- 208000032139 Halitosis Diseases 0.000 description 7
- 239000000463 material Substances 0.000 description 7
- 235000009508 confectionery Nutrition 0.000 description 6
- 230000000844 anti-bacterial effect Effects 0.000 description 5
- 208000002925 dental caries Diseases 0.000 description 5
- 230000002401 inhibitory effect Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 150000003464 sulfur compounds Chemical class 0.000 description 4
- 208000002064 Dental Plaque Diseases 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 231100000915 pathological change Toxicity 0.000 description 3
- 230000036285 pathological change Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- WQZGKKKJIJFFOK-SVZMEOIVSA-N (+)-Galactose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-SVZMEOIVSA-N 0.000 description 2
- MJVAVZPDRWSRRC-UHFFFAOYSA-N Menadione Chemical compound C1=CC=C2C(=O)C(C)=CC(=O)C2=C1 MJVAVZPDRWSRRC-UHFFFAOYSA-N 0.000 description 2
- 241000736262 Microbiota Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 235000013361 beverage Nutrition 0.000 description 2
- 235000015218 chewing gum Nutrition 0.000 description 2
- 229940112822 chewing gum Drugs 0.000 description 2
- 238000004891 communication Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000001066 destructive effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 235000015168 fish fingers Nutrition 0.000 description 2
- 230000003902 lesion Effects 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 201000001245 periodontitis Diseases 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000012552 review Methods 0.000 description 2
- -1 saccharide alcohols Chemical class 0.000 description 2
- 238000001356 surgical procedure Methods 0.000 description 2
- 229940034610 toothpaste Drugs 0.000 description 2
- 239000000606 toothpaste Substances 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 208000028804 PERCHING syndrome Diseases 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- WQZGKKKJIJFFOK-FPRJBGLDSA-N beta-D-galactose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-FPRJBGLDSA-N 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 229940046011 buccal tablet Drugs 0.000 description 1
- 239000006189 buccal tablet Substances 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 235000019219 chocolate Nutrition 0.000 description 1
- 235000020965 cold beverage Nutrition 0.000 description 1
- 230000005757 colony formation Effects 0.000 description 1
- 235000014510 cooky Nutrition 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 235000021185 dessert Nutrition 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000013332 fish product Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 210000004195 gingiva Anatomy 0.000 description 1
- BTIJJDXEELBZFS-QDUVMHSLSA-K hemin Chemical compound CC1=C(CCC(O)=O)C(C=C2C(CCC(O)=O)=C(C)\C(N2[Fe](Cl)N23)=C\4)=N\C1=C/C2=C(C)C(C=C)=C3\C=C/1C(C)=C(C=C)C/4=N\1 BTIJJDXEELBZFS-QDUVMHSLSA-K 0.000 description 1
- 229940025294 hemin Drugs 0.000 description 1
- 235000015243 ice cream Nutrition 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 235000011962 puddings Nutrition 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 235000013580 sausages Nutrition 0.000 description 1
- 235000011888 snacks Nutrition 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 108010050327 trypticase-soy broth Proteins 0.000 description 1
- 235000012711 vitamin K3 Nutrition 0.000 description 1
- 239000011652 vitamin K3 Substances 0.000 description 1
- 229940041603 vitamin k 3 Drugs 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/60—Sweeteners
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G1/00—Cocoa; Cocoa products, e.g. chocolate; Substitutes therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G1/00—Cocoa; Cocoa products, e.g. chocolate; Substitutes therefor
- A23G1/30—Cocoa products, e.g. chocolate; Substitutes therefor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G9/00—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
- A23L29/37—Sugar alcohols
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L9/00—Puddings; Cream substitutes; Preparation or treatment thereof
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L9/00—Puddings; Cream substitutes; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/92—Oral administration
Abstract
The purpose of the present invention is to provide a substance which can inhibit the proliferation of oral bacteria and is safe for ingestion. Particularly, the usefulness of the combined use of a saccharide and a sugar alcohol is not found clearly yet. Provided is a combination of a saccharide and a sugar alcohol, which can inhibit the proliferation of oral bacteria. An oral bacteria proliferation inhibitor is characterized by comprising galactose and sorbitol. This oral bacteria proliferation inhibitor is effective for the amelioration and prevention of periodontal disease.
Description
Technical field
The present invention relates to a kind of antiblastic of oral cavity bacterium.
Background technology
Allegedly in people's oral cavity, perching and reaching hundreds of antibacterials.Thereby wherein also comprise and multiplely carry out by causing inflammation at gingiva or periodontal or producing acid the bacterium that corrosion skeleton or tooth cause periodontal disease or dental caries.
Fusobacterium nucleatum (Fusobacterium nucleatum) (calling Fusobacterium nucleatum (F.Nucleatum) in the following text) is the bacterial species (non-patent literature 1,2) that can detect in a large number from the two dental plaque of healthy and disease position in people.The ratio of this bacterium increases along with the development of periodontal disease, therefore thinks that it contributes to morbidity and the development (non-patent literature 3～5) of periodontal disease.In addition, known its has the ability of adhering to other various bacteria, plays early stage colony formation strain and the later stage colony including periodontal pathogen are formed to the crosslinked effect (non-patent literature 6) that strain links together in dental plaque.Itself or as the generation strain of the volatile sulfur compounds of the reason material of halitosis.Therefore think, Fusobacterium nucleatum is demonstrated prevention, the minimizing of oral cavity bacterium and the prevention of halitosis of active antibacterial to periodontal disease, alleviated effectively.
For these bacterium are activated or suppress its active material and also study.For example well-known, reason material when saccharide is dental caries pathogen manufacture acid.On the other hand, known sugar alcohol has the inhibited proliferation to dental caries pathogen, has also reported in recent years the inhibited proliferation to periodontal pathogen.
Prior art document
Non-patent literature
Non-patent literature 1:Papapanou, P.N., Sellen, A., Wennstroem, and Dahlen J.L., G., choose at random the analysis (An analysis of the subgingival microflora in randomly selected subjects) of flora under the gum in object, " oral microorganism is learned and immunology (Oral microbiology and immunology) ", 8,24-29 (1993).
Non-patent literature 2:Moore, W.E. and Moore, L.V., the antibacterial (The bacteria of periodontal diseases) of periodontal, " periodontal (Periodontology) " 2000,5,66-77 (1994).
Non-patent literature 3:Dzink, J.L., Socransky, and Haffajee S.S., A.D., the activeness pathological changes of destructive periodontal and inactivity pathological changes mainly can cultivating microorganism (The predominant cultivable microbiota of active and inactive lesions of destructive periodontal diseases), " clinical periodontology magazine (Journal of clinical periodontology) ", 15,316-323 (1988).
Non-patent literature 4:Socransky, S.S., Haffajee, and Dzink A.D., J.L., the relation (Relationship of subgingival microbial complexes to clinical features at the sampled sites) under gum between microbe complex and the Clinical symptoms at sampling position, " clinical periodontology magazine ", 15,440-444 (1988).
Non-patent literature 5:Tanner, A. and Bouldin, H., the microorganism (The microbiota of early periodontitis lesions in adults) of the early stage periodontitis pathological changes in adult, " clinical periodontology magazine ", 16,467-471 (1989).
Non-patent literature 6:Kolenbrander, P.E., Andersen, R.N., Blehert, D.S., Egland, P.G., Foster, and Palmer J.S., R.J.Jr., the communication (Communication among oral bacteria) between oral cavity bacterium, " microbiology and molecular biology comment (Microbiology and molecular biology reviews) ", 66,486-505 (2002).
Non-patent literature 7:Krespi, Y.P., Shrime, and Kacker M.G., A., the relation (The relationship between oral malodor and volatile sulfur compound-producing bacteria) between halitosis and the antibacterial of generation volatile sulfur compounds, " otolaryngology-head and neck surgery (Otolaryngology-Head and Neck Surgery) ", 135,671-676 (2006).
Non-patent literature 8:Bolstad, A.I., Jensen, H.B. and Bakken, V., taxonomy, biology and the periodontal views (Taxonomy of Fusobacterium nucleatum, Biology, and Periodontal Aspects of Fusobacterium nucleatum), " clinical microbiology comment (Clinical Microbiology Reviews) ", 9,55-71 (1996).
Non-patent literature 9:Kapartral, V., Anderson, I., Ivanova, N., Reznik, G., Los, T., Lykidis, and Overbeek A., R., etc., the genome sequence of oral cavity bacterium Fusobacterium nucleatum ATCC25586 bacterial strain and analysis (Genome Sequence and Analysis of the Oral Bacterium Fusobacterium nucleatum Strain ATCC25586), " bacteriology's magazine (Journal of Bacteriology) ", 184,2005-2018 (2002).
Non-patent literature 10:Kapatral, V., Ivanova, N., Anderson, I., Reznik, G., Bhattacharyya A., Gardner, W.L. and Kyrpides, N., Deng, the genome analysis of Fusobacterium nucleatum Vincent subspecies and with the genomic comparison (Genome Analysis of F.nucleatum sub spp vincentii and Its Comparison With the Genome of F.nucleastum ATCC25586) of Fusobacterium nucleatum ATCC25586, " genome research (Genome Research) ", 6A, 1180-1189 (2003).
Summary of the invention
Invent technical problem to be solved
As periodontal pathogen and dental caries pathogen, known multiple oral cavity bacterium, requirement provides the material of the propagation that suppresses these bacterium.In addition, as such material, owing to working in oral cavity, even if therefore it is desirable to absorb to also harmless material in body.
In view of above-mentioned problem, absorb also harmless material even if the object of this invention is to provide a kind of propagation that suppresses oral cavity bacterium.Its object particularly provides a kind of combination about the serviceability of Saccharide and saccharide alcohols used time being it be unclear that, suppressed the Saccharide and saccharide alcohols of the propagation of oral cavity bacterium.
The technical scheme that technical solution problem adopts
The inventor has carried out conscientiously research, found that, in the culture medium that contains galactose and Sorbitol, the propagation of oral cavity bacterium Fusobacterium nucleatum is significantly suppressed, thereby has completed the present invention.That is, the present invention relates to a kind of antiblastic of oral cavity bacterium, it is characterized in that containing galactose and Sorbitol.
The effect of invention
By the antiblastic of oral cavity bacterium of the present invention is included in food or oral cavity composition, routine use or picked-up safely, can reduce oral cavity bacterium or improvement and prevention of periodontal disease effectively, can also prevent, alleviate halitosis.
Brief description of the drawings
Fig. 1 be represent for the each culture medium in embodiment 2 and the figure of OD value.
Fig. 2 be represent for the each culture medium in embodiment 3 and the figure of OD value.
Fig. 3 be represent for the each culture medium in embodiment 4 and the figure of OD value.
Detailed description of the invention
The antiblastic of the oral cavity bacterium that contains galactose and Sorbitol of the present invention has the effect of the propagation that suppresses synergistically Fusobacterium nucleatum.Fusobacterium nucleatum, by adhering to and become the reason that forms dental plaque with other various bacteria, therefore, by suppressing the propagation of this bacterium, can suppress the propagation of intraoral other antibacterial of the reason that becomes periodontal disease and dental caries.In addition, Fusobacterium nucleatum is one of pathogen of periodontal disease, and therefore the present invention is useful aspect the improvement of periodontal disease and prevention.And this bacterium produces the volatile sulfur compounds as the reason material of halitosis, therefore think, suppress the propagation of Fusobacterium nucleatum for the prevention of halitosis, alleviate also effective.
The total mol concentration of galactose and Sorbitol is preferably below the above 0.9M of 0.5M.In addition preferably galactose of the ratio of the molar concentration of galactose and Sorbitol: Sorbitol=2:1～1:3.
As galactose of the present invention, be better to use β-D-galactose.In addition as Sorbitol of the present invention, be better to use D-Sorbitol (D-sorbitol, D-sorbit).
In addition, antiblastic of the present invention can mix collutory, toothpaste, inhalant and contain the oral cavity compositions such as tablet, the dessert such as chewing gum, confection, pressed candy, soft sweet, chocolate, cookies and snacks, the cold drinks such as ice cream, popsicle and the freezing point heart, the poultry such as beverage, bread, scone, milk product, Petaso and sausage meat products class, or the fish product such as breaded fish stick and cylindric breaded fish stick, in the diet product such as non-staple foodstuff category, pudding, soup or fruit jam, use.
Embodiment 1
sample
Use D (+)-galactose (with the pure medicine of light (with light Pure medicine): superfine), D (-)-Sorbitol (with the pure medicine of light: one-level).
strains tested
Use Fusobacterium nucleatum JCM12990 strain as oral cavity bacterium.
culture medium
Use is added with the pancreas peptone soybean broth (Trypticase soy broth) (calling TSB culture medium in the following text) of yeast extract (3.0g/l), hemin (5.0mg/l), menadione (0.5mg/l), (10%CO under the anaerobic condition of 37 DEG C
2, 10%H
2, 80%N
2) cultivate.For containing galactose or Sorbitol or containing galactose and the culture medium of Sorbitol, preparation is dissolved with the TSB culture medium of galactose, Sorbitol, so that the ultimate density add 3.0ml in the TSB of 7.0ml culture medium time reaches setting concentration.Whole culture medium are carried out to filter (IDEC (ADVANTEC), m) sterilizing of hole shape 0.20 μ.
the mensuration of bacterium number
The preculture liquid of oral cavity bacterium Fusobacterium nucleatum JCM12990 is added in TSB culture medium 7.0ml, is cultured to the logarithmic proliferation phase.Remove the culture fluid of equivalent when adding preculture liquid, temporarily make liquid measure reach after 7.0ml, add containing galactose, Sorbitol or containing galactose and the TSB culture medium 3.0ml of Sorbitol of preparing, so that the concentration in 10.0ml reaches setting concentration.Now, by add containing in galactose and Sorbitol any ordinary culture medium 3.0ml sample in contrast.They were under anaerobic cultivated after 1 hour, culture test tube is moved in small scale shaker culture apparatus (the Biophotorecorder TVS062CA of IDEC Toyo Co., Ltd. (ア De バ Application テ ッ ク East ocean Co., Ltd.)), at 37 DEG C, cultivate after 24 hours, carry out the mensuration (U-3900H of Hitachi (HITACHI) spectrophotometer) of optical concentration (optical density: call OD value in the following text) with wavelength 660nm.
Embodiment 2
In embodiment 1, the Sorbitol of the galactose interpolation TSB culture medium of the galactose that preparation contains 0.1M or 0.6M, the Sorbitol that contains 0.2M, 0.3M or 0.6M adds TSB culture medium, the galactose that contains 0.4M and Sorbitol, the galactose of 0.3M and the galactose of the Sorbitol of 0.3M or 0.3M of 0.2M and TSB culture medium and contrast are added in the mixing of the Sorbitol of 0.6M, tests.For each TSB culture medium and OD value be shown in Fig. 1.With the TSB culture medium culturing of the galactose that contains 0.1M or 0.6M 24 hours, consequently OD value was significantly higher than the OD value of contrast.Report Fusobacterium nucleatum metabolism galactose (non-patent literature 8～10), infer because its impact makes propagation and promoted.In addition, while cultivating respectively 24 hours by the TSB culture medium of the Sorbitol that contains 0.2M, 0.3M or 0.6M, under the condition of 0.2M, confirm the OD value higher than contrast, under the condition of 0.3M, confirm and the OD value that contrasts same degree, under the condition of 0.6M, confirm the OD value lower than contrast.
Here, add TSB culture medium culturing 24 hours with the galactose of the 0.4M that contains the promotion that can predict propagation and the mixing that confirms the Sorbitol of the 0.2M of the promotion of propagation, consequently, confirm the OD value lower than contrast.Similarly, for containing 0.3M galactose and 0.3M Sorbitol and containing 0.3M galactose and the culture medium condition of 0.6M Sorbitol, also confirm the OD value lower than contrast, and this value is lower than the value under the condition of independent 0.3M Sorbitol, 0.6M Sorbitol.
Can be confirmed by above result, by making to contain galactose and Sorbitol in culture medium, the propagation of Fusobacterium nucleatum is suppressed.
Embodiment 3
By embodiment 2, the proliferation inhibiting effect of Fusobacterium nucleatum while having confirmed galactose and Sorbitol to be simultaneously added in culture medium, therefore by changing the ratio of galactose and the Sorbitol that can confirm this effect, examine whether to confirm same proliferation inhibiting effect.In addition, the total mol concentration of galactose and Sorbitol is set as 0.6M.For each TSB culture medium and OD value be shown in Fig. 2.
Consequently, at galactose: confirm the OD value lower than contrast under each ratio condition of Sorbitol=2:1,1:1,1:2,1:3.The degree that propagation suppresses does not rely on any the concentration in galactose or Sorbitol, under the condition of galactose: Sorbitol=1:2 (galactose: Sorbitol=0.2M:0.4M), confirms the strongest proliferation inhibiting effect.
Embodiment 4
Galactose for confirm the strongest proliferation inhibiting effect in embodiment 3: the condition of Sorbitol=1:2, ratio is fixed, change total mol concentration and carry out same test.For each TSB culture medium and OD value be shown in Fig. 3.
Consequently, be the condition of 0.3M, 0.4M for total mol concentration, confirm the OD value higher than contrast.On the other hand, be the condition of 0.5M, 0.6M, 0.9M for total mol concentration, confirm the OD value lower than contrast.The degree that propagation suppresses does not rely on total mol concentration, under the condition of 0.6M (galactose: Sorbitol=0.2M:0.4M), confirms the strongest proliferation inhibiting effect.
Embodiment 5
The present embodiment illustrates representational Formulation Example of the present invention below, but the present invention is not limited to this.
Manufacture collutory according to following formula.
Embodiment 6
Manufacture toothpaste according to following formula.
Embodiment 7
Manufacture halitosis spray according to following formula.
Embodiment 8
Manufacture buccal tablet according to following formula.
Embodiment 9
Manufacture chewing gum according to following formula.
Embodiment 10
Manufacture confection according to following formula.
Embodiment 11
Manufacture pressed candy according to following formula.
Embodiment 12
Manufacture soft sweet according to following formula.
Embodiment 13
Manufacture beverage according to following formula.
The application requires the priority of No. 2011-145737th, Japanese patent application based on filing an application on June 30th, 2011, quotes the part of its content as the application.
Claims (7)
1. an antiblastic for oral cavity bacterium, is characterized in that, contains galactose and Sorbitol.
2. the antiblastic of oral cavity bacterium as claimed in claim 1, is characterized in that, contains galactose and Sorbitol to amount to the concentration of 0.5M～0.9M.
3. the antiblastic of oral cavity bacterium as claimed in claim 1 or 2, is characterized in that, galactose: the ratio of Sorbitol is in the scope of 2:1～1:3.
4. the antiblastic of the oral cavity bacterium as described in any one in claim 1～3, is characterized in that, oral cavity bacterium is periodontal pathogen.
5. the antiblastic of the oral cavity bacterium as described in any one in claim 1～4, is characterized in that, oral cavity bacterium is Fusobacterium nucleatum.
6. an oral cavity composition, is characterized in that, the antiblastic that contains the oral cavity bacterium described in any one in claim 1～5.
7. a food, is characterized in that, the antiblastic that contains the oral cavity bacterium described in any one in claim 1～5.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2011-145737 | 2011-06-30 | ||
| JP2011145737A JP2013010724A (en) | 2011-06-30 | 2011-06-30 | Oral bacteria proliferation inhibitor |
| PCT/JP2012/004187 WO2013001817A1 (en) | 2011-06-30 | 2012-06-28 | Oral bacteria proliferation inhibitor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104039304A true CN104039304A (en) | 2014-09-10 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201280032036.7A Pending CN104039304A (en) | 2011-06-30 | 2012-06-28 | Oral bacteria proliferation inhibitor |
Country Status (5)
| Country | Link |
|---|---|
| JP (1) | JP2013010724A (en) |
| KR (1) | KR20140061349A (en) |
| CN (1) | CN104039304A (en) |
| TW (1) | TW201313234A (en) |
| WO (1) | WO2013001817A1 (en) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106794191A (en) * | 2014-09-24 | 2017-05-31 | 首尔大学校产学协力团 | Quorum sensing inhibitor containing D galactolipins |
| EP3466403A1 (en) * | 2017-10-04 | 2019-04-10 | Prisman GmbH | Use of a monosaccharide for sub and/or supragingival cleaning of teeth |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0217120A (en) * | 1988-05-14 | 1990-01-22 | Biodyn Ag | Dental caries preventing agent |
| US20030103912A1 (en) * | 2001-10-25 | 2003-06-05 | Levin Gilbert V. | D-tagatose as an anti-biofilm agent |
| CN101027034A (en) * | 2004-09-29 | 2007-08-29 | 花王株式会社 | Composition for oral cavity |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006182692A (en) * | 2004-12-27 | 2006-07-13 | Kao Corp | Composition for oral cavity |
| JP2008303188A (en) * | 2007-06-08 | 2008-12-18 | Univ Of Tokushima | Candida biofilm eliminating agent |
-
2011
- 2011-06-30 JP JP2011145737A patent/JP2013010724A/en active Pending
-
2012
- 2012-06-28 WO PCT/JP2012/004187 patent/WO2013001817A1/en active Application Filing
- 2012-06-28 KR KR1020147001513A patent/KR20140061349A/en not_active Application Discontinuation
- 2012-06-28 CN CN201280032036.7A patent/CN104039304A/en active Pending
- 2012-06-29 TW TW101123499A patent/TW201313234A/en unknown
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0217120A (en) * | 1988-05-14 | 1990-01-22 | Biodyn Ag | Dental caries preventing agent |
| US20030103912A1 (en) * | 2001-10-25 | 2003-06-05 | Levin Gilbert V. | D-tagatose as an anti-biofilm agent |
| CN101027034A (en) * | 2004-09-29 | 2007-08-29 | 花王株式会社 | Composition for oral cavity |
Also Published As
| Publication number | Publication date |
|---|---|
| TW201313234A (en) | 2013-04-01 |
| JP2013010724A (en) | 2013-01-17 |
| KR20140061349A (en) | 2014-05-21 |
| WO2013001817A1 (en) | 2013-01-03 |
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Application publication date: 20140910 |