CN104031974B - A kind of sex appraisal method of Veneridae shellfish parent - Google Patents
A kind of sex appraisal method of Veneridae shellfish parent Download PDFInfo
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- CN104031974B CN104031974B CN201410202435.4A CN201410202435A CN104031974B CN 104031974 B CN104031974 B CN 104031974B CN 201410202435 A CN201410202435 A CN 201410202435A CN 104031974 B CN104031974 B CN 104031974B
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Abstract
nullA kind of sex appraisal method of Veneridae shellfish parent，Follow the steps below: prepare the syringe with syringe needle，Prepare Veneridae shellfish to be identified，Find external ligament end and the plotted point of shell edge of Veneridae shellfish，This point is collection point，One hand holds steady Veneridae shellfish to be identified，With another hands by the tip alignment collection point of syringe，Allowing needle point and shell edge tangent line is 30 60 degree of angles，Needle point is sent in Veneridae shellfish to be identified to gonad position by collection point，Draw sample，Putting samples into drip has on the microscope slide of sea water，The form of microexamination sample，As sample dissipates in the seawater，And the ovum of circle or pyriform can be seen，Illustrate that identified Veneridae shellfish is female，It is smoke-like in the seawater such as sample，And the sperm of Tinea Ranae shape can be seen，Illustrate that identified Veneridae shellfish is male，As the ovum of circle or pyriform can be seen，The sperm of Tinea Ranae shape can be seen again，Illustrate that identified Veneridae shellfish is hermaphroditism.
Description
Technical field
The invention belongs to field of marine biotechnology, relate in particular to the sex appraisal method of a kind of Veneridae shellfish parent.
Background technology
In the shellfish of China's cultivation, Veneridae shellfish is in occupation of important cultivation status.Such as, the Ruditapes philippinarum that Veneridae clam son belongs to is one of big cultivated shellfish of China four, has the highest economic worth.Saxidomus purpurtus common on market, Conch Meretricis seu Cyclinae, sand clam, thin slice mirror clam broadly fall into Veneridae shellfish.Veneridae shellfish mostly is bisexualism, and hermaphroditism occurs in minority individuality.In dioecious shellfish, because male and female similar shape i.e. secondary sex characteristics zero difference, so being difficult to identify sex by profile.And shellfish existence reversal, bisexualism can exchange with sex, and hermaphroditism also property can become bisexualism, makes sex identification extremely difficult.So that waterman author is carrying out the nearly edge of shellfish, distant hybridization, family foundation, especially when colony hybridization and Seedling production, bring huge difficulty.
At present, research for shellfish sex appraisal method, documents (patent publication No. is CN 101377485A) discloses a kind of Miniature electric drill punch method and identifies shellfish property method for distinguishing, this documents utilizes specific purpose tool to hole on shellfish parent shell, then from hole, take out essence or ovum with syringe, and microscopy determines parent's sex types under the microscope.Although documents has universal versatility to the bisexualism type shellfish of None-identified, but need special equipment (Miniature electric drill), cause the raising of experimentation cost, and whole experimentation is relatively complicated, complicated, the most important thing is that the shell that need to identify can be caused damage by this method, be unfavorable for the continuation survival of identified individual.
Summary of the invention
The present invention is to solve the above-mentioned deficiency existing for prior art, proposes a kind of method that on the premise of not destroying shellfish housing, shellfish can be carried out sex identification.
The technical solution of the present invention is: the sex appraisal method of a kind of Veneridae shellfish parent, it is characterised in that: described method follows the steps below:
A, preparation, with the syringe of syringe needle, prepare Veneridae shellfish to be identified,
B, the external ligament end finding Veneridae shellfish and the plotted point of shell edge, this point is collection point,
C, one hand hold steady Veneridae shellfish to be identified, and with another hands by the tip alignment collection point of syringe, allowing needle point and shell edge tangent line is 30-60 degree angle, are sent in Veneridae shellfish to be identified to gonad position by collection point by needle point, absorption sample,
D, put samples into drip have on the microscope slide of sea water, the form of microexamination sample, as dissipated in sample sea water, and the ovum of circle or pyriform can be seen, illustrate that identified Veneridae shellfish is female, it is smoke-like in the seawater such as sample, and the sperm of Tinea Ranae shape can be seen, illustrate that identified Veneridae shellfish is male, as the ovum of circle or pyriform can be seen, the sperm of Tinea Ranae shape can be seen again, illustrate that identified Veneridae shellfish is hermaphroditism.
Syringe in described a step can be disposable sterilized injector, it is also possible to for sterile-processed syringe.
The present invention compared with the existing technology, has the advantage that
The sex appraisal method of Veneridae shellfish parent disclosed in this invention, need not the shell of Veneridae shellfish is destroyed, operation just can be completed with instrument simple and easy to get, its qualification process is simple, promote easily, and the shellfish after Jian Dinging is without any damage, can be with normal spawning row's essence, waterman author is being carried out the nearly edge of shellfish, distant hybridization, family foundation by its appearance, particularly colony hybridization and Seedling production is had laid a good foundation, and has important directive significance for shellfish genetic breeding.It can be said that it has possessed multiple advantage, being particularly suitable for popularization and application in the art, its wide market, significance of scientific research is great.
Accompanying drawing explanation
Fig. 1 is Veneridae shellfish sand clam sex identification embodiment plane of structure figure and vertical face figure.
Fig. 2 is Veneridae shellfish Conch Meretricis seu Cyclinae sex identification embodiment plane of structure figure and vertical face figure.
Fig. 3 is Veneridae shellfish Saxidomus purpurtus sex identification embodiment plane of structure figure and vertical face figure.
Detailed description of the invention
The detailed description of the invention of the present invention is described below in conjunction with accompanying drawing.
A kind of sex appraisal method of Veneridae shellfish parent, follow the steps below: first prepare the syringe with syringe needle, here syringe can select disposable sterilized injector, the reusable syringe processed through exterminating bacterium can also be selected, it is ready for Veneridae shellfish to be identified, shell finds its external ligament end and the plotted point of shell edge, this point is collection point；Then singlehanded holding steady Veneridae shellfish to be identified, with another hands by the tip alignment collection point of syringe, allowing the tangent line at needle point and shell edge is the angle of 30-60 degree, and in needle point is sent into shell according to this angle until its gonad position, and draws sample；Being instilled by the sample of acquisition has on the microscope slide of sea water, examine under a microscope the form of sample, if sample dissipates in the seawater, and it can be seen that the circular or ovum of pyriform, illustrate that identified Veneridae shellfish is female, if sample is smoke-like in the seawater, and the sperm of Tinea Ranae shape can be seen, illustrate that identified Veneridae shellfish is male, as the ovum of circle or pyriform can be seen, the sperm of Tinea Ranae shape can be seen again, illustrate that identified Veneridae shellfish is hermaphroditism.
Embodiment 1
In 2013, in Liaoning Province's shellfish stock breeding Engineering Technical Research Centre, by presently disclosed method, sand clam sex is identified.As shown in Figure 1.
First preparing a disposable sterilized injector, its range size is 1mL；Prepare sand clam to be identified, and determine external ligament end and the shell edge plotted point of this sand clam, this point is exactly collection point, left hand holds steady sand clam, the tip alignment collection point of right hand syringe, adjusting needle point with shell edge tangent line angle is 35 degree, and the needle point of syringe is sent into the soft body of sand clam, size according to sand clam allows needle point enter the gonad position of sand clam, draws sample；
Putting into the sample of taking-up to drip in advance has on the microscope slide of sea water, then examines under a microscope the form of sample, thus differentiates sex.If sample dissipates in the seawater, and it can be seen that the circular or ovum of pyriform, illustrate that identified Veneridae shellfish is female, if sample is smoke-like in the seawater, and the sperm of Tinea Ranae shape can be seen, illustrate that identified Veneridae shellfish is male, as the ovum of circle or pyriform can be seen, the sperm of Tinea Ranae shape can be seen again, illustrate that identified Veneridae shellfish is hermaphroditism.
Differentiating 160 sand clam through the method, female 103, male 57, discrimination reaches the sand clam energy normal growth survival after 100%, and discriminating and breeding.
Embodiment 2
In 2013, in Liaoning Province's shellfish stock breeding Engineering Technical Research Centre, by presently disclosed method, Conch Meretricis seu Cyclinae sex is identified.As shown in Figure 2.
First preparing ordinary syringe, its range size is 1mL, and to its disinfection；Prepare Conch Meretricis seu Cyclinae to be identified, and determine external ligament end and the shell edge plotted point of this Conch Meretricis seu Cyclinae, this point is exactly collection point, left hand holds steady Conch Meretricis seu Cyclinae, the tip alignment collection point of right hand syringe, adjusting needle point with shell edge tangent line angle is 55 degree, and the needle point of syringe is sent into the soft body of Conch Meretricis seu Cyclinae, size according to Conch Meretricis seu Cyclinae allows needle point enter the gonad position of Conch Meretricis seu Cyclinae, draws sample；
Putting into the sample of taking-up to drip in advance has on the microscope slide of sea water, then examines under a microscope the form of sample, thus differentiates sex.If sample dissipates in the seawater, and it can be seen that the circular or ovum of pyriform, illustrate that identified Veneridae shellfish is female, if sample is smoke-like in the seawater, and the sperm of Tinea Ranae shape can be seen, illustrate that identified Veneridae shellfish is male, as the ovum of circle or pyriform can be seen, the sperm of Tinea Ranae shape can be seen again, illustrate that identified Veneridae shellfish is hermaphroditism.
Differentiating 150 Conch Meretricis seu Cyclinaes through the method, female 91, male 59, discrimination reaches the Conch Meretricis seu Cyclinae energy normal growth survival after 100%, and discriminating and breeding.
Embodiment 3
In 2013, in Liaoning Province's shellfish stock breeding Engineering Technical Research Centre, by presently disclosed method, Saxidomus purpurtus sex is identified.As shown in Figure 3.
First preparing disposable sterilized injector, its range size is 1mL；Prepare Saxidomus purpurtus to be identified, and determine external ligament end and the shell edge plotted point of this Saxidomus purpurtus, this point is exactly collection point, left hand holds steady Saxidomus purpurtus, the tip alignment collection point of right hand syringe, adjusting needle point with shell edge tangent line angle is 45 degree, and the needle point of syringe is sent into the soft body of Saxidomus purpurtus, size according to Saxidomus purpurtus allows needle point enter the gonad position of Saxidomus purpurtus, draws sample；
Putting into the sample of taking-up to drip in advance has on the microscope slide of sea water, then examines under a microscope the form of sample, thus differentiates sex.If sample dissipates in the seawater, and it can be seen that the circular or ovum of pyriform, illustrate that identified Veneridae shellfish is female, if sample is smoke-like in the seawater, and the sperm of Tinea Ranae shape can be seen, illustrate that identified Veneridae shellfish is male, as the ovum of circle or pyriform can be seen, the sperm of Tinea Ranae shape can be seen again, illustrate that identified Veneridae shellfish is hermaphroditism.
Differentiating 180 Saxidomus purpurtus through the method, female 115, male 75, discrimination reaches the Saxidomus purpurtus energy normal growth survival after 100%, and discriminating and breeding.
Claims (1)
1. the sex appraisal method of a Veneridae shellfish parent, it is characterised in that: described method follows the steps below:
A. prepare the syringe with syringe needle, prepare Veneridae shellfish to be identified,
B. finding external ligament end and the plotted point of shell edge of Veneridae shellfish, this point is collection point,
C. singlehanded holding steady Veneridae shellfish to be identified, with another hands by the tip alignment collection point of syringe, allowing needle point and shell edge tangent line is 30-60 degree angle, is sent in Veneridae shellfish to be identified to gonad position by collection point by needle point, absorption sample,
D. putting samples into drip has on the microscope slide of sea water, the form of microexamination sample, as sample dissipates in the seawater, and the ovum of circle or pyriform can be seen, illustrate that identified Veneridae shellfish is female, it is smoke-like in the seawater such as sample, and the sperm of Tinea Ranae shape can be seen, illustrate that identified Veneridae shellfish is male, as the ovum of circle or pyriform can be seen, the sperm of Tinea Ranae shape can be seen again, illustrate that identified Veneridae shellfish is hermaphroditism.
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| CN105830958A (en) * | 2015-11-24 | 2016-08-10 | 福建省水产研究所 | Gender classification method of Ruditapes philippinarum parent scallop |
| CN105918183B (en) * | 2016-05-27 | 2019-04-30 | 中国海洋大学 | A kind of experiment Mytilus galloprovincialis living body sex identification and growth recovery method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101361469A (en) * | 2008-09-25 | 2009-02-11 | 大连水产学院 | Common intertidal shellfish polymerization hybridization breeding method |
| CN101377485A (en) * | 2008-09-23 | 2009-03-04 | 大连水产学院 | Method for identifying parent sex of shellfish |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101377485A (en) * | 2008-09-23 | 2009-03-04 | 大连水产学院 | Method for identifying parent sex of shellfish |
| CN101361469A (en) * | 2008-09-25 | 2009-02-11 | 大连水产学院 | Common intertidal shellfish polymerization hybridization breeding method |
Non-Patent Citations (1)
| Title |
|---|
| 闫喜武等.菲律宾蛤仔莆田群体与大连群体生物学比较.《生态学报》.2005,第25卷(第12期),3329-3334. * |
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