CN104029234A - Stem cutting device for tissue culture seedlings and application of stem cutting device - Google Patents

Stem cutting device for tissue culture seedlings and application of stem cutting device Download PDF

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Publication number
CN104029234A
CN104029234A CN201410273140.6A CN201410273140A CN104029234A CN 104029234 A CN104029234 A CN 104029234A CN 201410273140 A CN201410273140 A CN 201410273140A CN 104029234 A CN104029234 A CN 104029234A
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China
Prior art keywords
drawer
tissue culture
blade
group training
pull bar
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CN201410273140.6A
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Chinese (zh)
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CN104029234B (en
Inventor
王海荣
安淼
余贤美
刘加芬
张安宁
刘伟
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Shandong Luguo Modern Facility Fruit Tree Technology Co ltd
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Shandong Institute of Pomology
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Priority to CN201410273140.6A priority Critical patent/CN104029234B/en
Publication of CN104029234A publication Critical patent/CN104029234A/en
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Publication of CN104029234B publication Critical patent/CN104029234B/en
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  • Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)

Abstract

The invention relates to a stem cutting device for tissue culture seedlings and application of the stem cutting device. The stem cutting device comprises a tank, a first drawer and a second drawer. The first drawer and the second drawer are arranged in the tank, a left board and a right board of the tank are of opening structures, a cutter device and a cutting board device are oppositely arranged on the inner sides of the left board of the tank, the first drawer is arranged below the cutter device and the cutting board device, and the second drawer is adjacently arranged on a side of the first drawer. The stem cutting device and the application have the advantages that the cutter device and the cutting board device are used with each other, so that stems of the tissue culture seedlings can be cut, the traditional manual cutting mode can be replaced by the stem cutting device in a mechanical mode, accordingly, resources can be saved, and the efficiency can be improved; the cutter device and the cutting board device of the stem cutting device can be replaced for different tissue culture seedlings, accordingly, cross contamination can be prevented, the cleanliness of the aseptic tissue culture seedlings can be improved, obvious effects can be realized, and the stem cutting device and the application have excellent economic value and excellent social benefits.

Description

A kind of tissue culture seeding stem segment cutter sweep and application thereof
Technical field
The present invention relates to a kind of tissue culture seeding stem segment cutter sweep and application thereof, belong to field of plant tissue culture technique.
Background technology
At present, when plant being organized to cultivation, need to process group training seedling, comprise cutting apart tissue culture seeding stem segment, the removal of the aging tissue in plant bottom, in plants stems, yellow leaf part wipes out etc., these be all set up plant regeneration system must be through step, but these step major parts are to carry out in sterilized culture dish at present, need manually with scissors and tweezers, every strain group training seedling repeatedly to be pruned, in order to inoculation, use, there is following shortcoming in this traditional tissue culture seeding stem segment processing mode: 1, treatment step is more loaded down with trivial details, and manual work wastes time and energy, inefficiency, 2,, in the process of in manual work, the aging tissue of stem section and bottom of group training seedling being removed, be easy to cause the problems such as tissue culture seedling pollution.
Summary of the invention
For the deficiencies in the prior art, the invention provides a kind of tissue culture seeding stem segment cutter sweep.
The present invention also provides a kind of using method of tissue culture seeding stem segment cutter sweep described above.
Technical scheme of the present invention is as follows:
A kind of tissue culture seeding stem segment cutter sweep, comprise casing and be arranged on the first drawer and the second drawer in casing, wherein, upper boxboard and the left boxboard of described casing are all set to hatch frame, the left boxboard inner side of described casing is relatively set with cutter and knife plate device, described cutter and knife plate device are used in combination group training seedling are carried out to the cutting of stem section, described the first drawer be arranged on cutter and knife plate device under, the adjacent side that is arranged on the first drawer of described the second drawer.
According to the present invention, preferably, described cutter comprises pull bar chamber, pull bar and cutting knife body, and described pull bar chamber is set to rectangle cavity, in described cavity, be provided with the pull bar passage passing for pull bar, described pull bar one end is fixedly connected with cutting knife body after running through pull bar passage.
Further preferred, described pull bar passage is downward-sloping setting from outside to inside.In actual use, the blade arranging on cutting knife body cuts off group training seedling on knife plate device, for preventing blade cut-out group completely training seedling, therefore by the downward-sloping certain angle that arranges of pull bar passage, when blade contacts with knife plate, the bottom of blade flushes with the base of knife plate;
Described cutting knife body comprises movable plate, adjusting rod, two expansion links, fixed bar, connecting rod, support bar, blade holder and blades, described fixed bar is fixedly connected with support bar is vertical, described support bar is fixedly connected with movable plate, the two ends of described expansion link are fixedly linked with adjusting rod and fixed bar respectively, described two expansion link horizontal symmetrical arrange, between the symmetrical node of described two expansion links, be provided with connecting rod, described connecting rod and blade holder are fixedly linked, and in described blade holder, are provided with blade.
Further preferred, the two ends of described connecting rod are connected with expansion link by bearing pin, and described blade holder is set to the cuboid with groove, an end opening of described groove, and described blade inserts and is arranged in groove along opening.The benefit of this design is, described blade is detachable blade, on the one hand, for different group training seedlings, uses different blades, guaranteed the cleanliness factor in operation process; On the other hand, for the blade of wearing and tearing rust, can change in time.
Described support bar is set to a rectangular flat plate, is provided with a hollow slideway in described dull and stereotyped horizontal direction, on described adjusting rod and connecting rod, is provided with projection, and described projection is embedded into and in hollow slideway, carries out horizontal slip.The benefit of this design is, on support bar, be provided with hollow slideway, projection on adjusting rod and connecting rod moves in hollow slideway, can guarantee adjusting rod and the connecting rod stability in moving process, can play the fixation up and down to adjusting rod and connecting rod simultaneously.
The top of described adjusting rod is higher than the top edge of movable plate, and the top of described adjusting rod is provided with handle.The benefit of this design is, adjusting rod is higher than movable plate, be conducive to operating personnel and conveniently hold handle push-and-pull adjusting rod and carry out adjustable telescopic rod, and then the distance between adjustable blade.
According to the present invention, preferred, described knife plate device comprises knife plate frame and is arranged on the knife plate on knife plate frame.The object of this design is, knife plate is in order to bear the cutting of blade to tissue culture seeding stem segment.
Further preferred, described knife plate pulls and pushes and is arranged on knife plate frame.The benefit of this design is, for different group training seedlings, can use different knife plates, prevents the mutual pollution between different group training seedlings.
According to the present invention, preferred, described casing also comprises a dividing plate, and described dividing plate is arranged between the first drawer and the second drawer.The benefit of this design is, in actual job process, the first drawer is in order to deposit the stem section after cutting, and the second drawer is in order to deposit the discarded parts such as group training seedling bottom, and the dividing plate of setting up has been avoided the pollution of the second drawer to the first drawer.
According to the present invention, preferred, the height of described left boxboard, dividing plate and the second drawer is all lower than the first drawer.The object of this design is, the maximized cutting process for group training seedling provides a free of contamination environment, and minimizing group training seedling contacts with other objects.
According to the present invention, preferably, four edges of described the first drawer left plate are symmetrically arranged with pulley, and front side board and the back side panel of described the first drawer are symmetrically arranged with slideway, described pulley embeds and is arranged in slideway, and the slip by pulley in slideway realizes the movement of left plate.The benefit of this design is, in order to meet the instructions for use of different length group training seedling, the group training seedling that need to regulate to realize different length to the width of the first drawer can be taken and be placed on the first drawer, and this design can easily realize the adjusting of the first drawer width by the movement of left plate.
A using method for tissue culture seeding stem segment cutter sweep, step is as follows,
(1) with sterilized tweezers, get the group training seedling with obvious internode and be placed on the first drawer, and make group training seedling near knife plate, the bottom of group training seedling is placed in the top of the second drawer;
(2) slow pushing drawing rod inwards, blade moves towards knife plate direction, when blade approaches group training seedling, stops pushing drawing rod, by the distance between adjusting rod timely adjustment blade, makes blade in suitable position;
(3) pushing drawing rod inwards rapidly, blade is cut into multistage by group training seedling, and the stem section of group training seedling falls in the first drawer, and the bottom of group training seedling falls in the second drawer;
(4) pull out pull bar, repeat above step.
Beneficial effect of the present invention is:
1. the present invention has abandoned the manual mode that tradition is cut tissue culture seeding stem segment, with motorisation unit, has realized cutting operation, has saved manpower and materials, has improved operating efficiency.
2. the present invention has reduced the pollution rate under traditional cutting mode, tissue culture seeding stem segment and bottom are placed in different operating areas, incisory blade, knife plate, drawer can be changed at any time simultaneously, and the asepticize that carries out disinfection is processed, and have improved the cleanliness factor of operating environment.
3. in apparatus of the present invention, with blade, knife plate, the drawer of the contact of aseptic group of training seedling, can change at any time, guarantee being repeatedly used of a set of sterile device.
4. tissue culture seeding stem segment cutter sweep of the present invention design concept is ingenious, simple to operate, easy to use, effect is obvious, effect is remarkable, has good economic worth and social benefit, is worthy of popularization.
Accompanying drawing explanation
Fig. 1 is the overall structure schematic diagram of cutter sweep of the present invention;
Fig. 2 is the overall structure schematic diagram of the cutter sweep of band dividing plate of the present invention;
Fig. 3 is the stereogram that in the present invention, chest portion is divided;
Fig. 4 is with the stereogram of the casing part of dividing plate in the present invention;
Fig. 5 is the front view of casing in the present invention;
Fig. 6 is the left view of casing in the present invention;
Fig. 7 is the structural representation that in the present invention, cutting knife body matches with pull bar chamber;
Fig. 8 is the structural representation matching with pull bar chamber during the operation of cutting knife body in the present invention;
Fig. 9 is the front view of cutting knife body in the present invention;
Figure 10 is the rearview of cutting knife body in the present invention;
Figure 11 is the front view of connector and blade holder structural relation in the present invention;
Figure 12 is the left view of connector and blade holder structural relation in the present invention;
Figure 13 is the structural representation of cutter sweep of the present invention while carrying out operation;
Figure 14 is the front view of the present invention's the first drawer;
Figure 15 is the top view of the present invention's the first drawer;
Figure 16 is the left view of the present invention's the first drawer;
Figure 17 is the left side perspective view of the present invention's the first drawer;
Figure 18 is the left view when the left plate of the present invention's the first drawer;
Figure 19 is the left side perspective view when the left plate of the present invention's the first drawer;
Figure 20 is the structural representation of the present invention's the first drawer left plate.
Wherein: 1 is casing; 2 is the first drawer; 201 is left plate; 202 is slideway; 203 is pulley; 204 is front side board; 205 is back side panel; 3 is the second drawer; 4 is pull bar chamber; 5 is movable plate; 501 is adjusting rod; 502 is expansion link; 503 is fixed bar; 504 is connecting rod; 505 is support bar; 506 is handle; 507 is hollow slideway; 508 is projection; 509 is blade holder; 510 is blade; 6 is knife plate; 7 is knife plate frame; 8 is pull bar passage; 9 is blade; 10 is dividing plate; 11 is left boxboard; 12 is pull bar; 13 is the first workspace; 14 is secondary service area; 15 is group training seedling.
The specific embodiment
Below by embodiment, also the present invention will be further described by reference to the accompanying drawings, but be not limited to this.
Embodiment 1:
As shown in Figures 1 to 10, a kind of tissue culture seeding stem segment cutter sweep, comprise casing 1 and be arranged on the first drawer 2 and the second drawer 3 in casing, upper boxboard and the left boxboard 11 of described casing are all set to hatch frame, left boxboard 11 inner sides of described casing are relatively set with cutter and knife plate device, described cutter and knife plate device are used in combination group training seedling are carried out to the cutting of stem section, described the first drawer 2 be arranged on cutter and knife plate device under, described the second adjacent right side that is arranged on the first drawer 2 of drawer 3.While using this device, the position at the first drawer 2 places is the first workspace 13, the position at the second drawer 3 places is secondary service area 14, group training seedling is taken and is placed on the first drawer 2, the stem section of group training seedling is partly positioned at the first workspace 13, the bottom of group training seedling is positioned at secondary service area 14, by pushing drawing rod, moves cutting knife body, utilizes blade 9 to train the cutting of seedling with the group that coordinated of knife plate 6.
Wherein, described cutter comprises pull bar chamber 4, pull bar 12 and cutting knife body, described pull bar chamber 4 is set to rectangle cavity, in described cavity, be provided with the pull bar passage 8 passing for pull bar 12, described pull bar passage 8 is downward-sloping setting from outside to inside, and described pull bar 12 one end are fixedly connected with cutting knife body after running through pull bar passage 8;
Described cutting knife body comprises movable plate 5, adjusting rod 501, two expansion links 502, fixed bar 503, connecting rod 504, support bar 505, blade holder 509 and blade 510, the top of described adjusting rod 501 is higher than the top edge of movable plate 5, the top of described adjusting rod 501 is provided with handle 506, described fixed bar 503 is fixedly connected with support bar 505 is vertical, described support bar 505 is fixedly connected with movable plate 5, the two ends of described expansion link 502 are fixedly linked with adjusting rod 501 and fixed bar 503 respectively, described two expansion links, 502 horizontal symmetrical settings, between the symmetrical node of described two expansion links 502, be provided with connecting rod 504, the two ends of described connecting rod 504 are connected with expansion link 502 by bearing pin, described connecting rod 504 is fixedly linked with blade holder 509, in described blade holder 509, be provided with blade 510.
Wherein, described support bar 505 is set to a rectangular flat plate, in described dull and stereotyped horizontal direction, be provided with a hollow slideway 507, on described adjusting rod 501 and connecting rod 504, be provided with projection 508, described projection 508 is embedded into and in hollow slideway 507, carries out horizontal slip.
Wherein, described knife plate device comprises knife plate frame 7 and is arranged on the knife plate 6 on knife plate frame 7, and described knife plate 6 pulls and pushes and is arranged on knife plate frame 7, and knife plate 7 is in order to bear the cutting of blade to tissue culture seeding stem segment.
Embodiment 2:
As shown in Figure 11 and Figure 12, a kind of tissue culture seeding stem segment cutter sweep, structure is as described in Example 1, its difference is, described blade holder 509 is set to the cuboid with groove, an end opening of described groove, and described blade 510 inserts and is arranged in groove along opening.
Embodiment 3:
As shown in Figure 2 and Figure 4, a kind of tissue culture seeding stem segment cutter sweep, structure as described in Example 1, its difference is, described casing 1 also comprises a dividing plate 10, described dividing plate 10 is arranged between the first drawer 2 and the second drawer 3, and the height of described left boxboard 11, dividing plate 10 and the second drawer 3 is all lower than the first drawer 2.In actual job process, the first drawer 2 is in order to deposit the stem section after cutting, the second drawer 3 is in order to deposit the root of group training seedling, the dividing plate 10 of setting up has been avoided the pollution of 3 pairs of the first drawers 2 of the second drawer, the maximized cutting process for group training seedling provides a free of contamination environment, and minimizing group training seedling contacts with other objects.
Embodiment 4:
As shown in Figure 14 to Figure 20, a kind of tissue culture seeding stem segment cutter sweep, structure as described in Example 3, its difference is, four edges of described the first drawer left plate 201 are symmetrically arranged with pulley 203, front side board 204 and the back side panel 205 of described the first drawer are symmetrically arranged with slideway 202, and described pulley 203 embeds and is arranged in slideway 202, and the slip by pulley 203 in slideway 202 realizes the movement of left plate 201.For different length group training seedling, by the movement of left plate, regulate the width of the first drawer, group training seedling can be taken better and be placed on the first drawer.
Embodiment 5:
A method of using cutter sweep described above to carry out the cutting of stem section to pomegranate group training seedling, step is as follows,
(1) with sterilized tweezers, get the pomegranate group training seedling with obvious internode and be placed on the first drawer 2, and make group training seedling near knife plate 6, the bottom of group training seedling is placed in the top of the second drawer 3;
(2) slow pushing drawing rod 12 inwards, blade 9 moves towards knife plate 6 directions, when blade 9 approaches group training seedling, stops pushing drawing rod 12, and the distance by between adjusting rod 501 timely adjustment blades 9, makes blade 9 in suitable position;
(3) pushing drawing rod 12 inwards rapidly, blade 9 is cut into multistage by group training seedling, and the stem section of group training seedling falls into 2 li, the first drawer, and the bottom of group training seedling falls into 3 li, the second drawer;
(4) pull out pull bar 12, repeat above step.

Claims (10)

1. a tissue culture seeding stem segment cutter sweep, comprise casing and be arranged on the first drawer and the second drawer in casing, it is characterized in that, upper boxboard and the left boxboard of described casing are all set to hatch frame, the left boxboard inner side of described casing is relatively set with cutter and knife plate device, described cutter and knife plate device are used in combination group training seedling are carried out to the cutting of stem section, described the first drawer be arranged on cutter and knife plate device under, the adjacent side that is arranged on the first drawer of described the second drawer.
2. tissue culture seeding stem segment cutter sweep as claimed in claim 1, it is characterized in that, described cutter comprises pull bar chamber, pull bar and cutting knife body, described pull bar chamber is set to rectangle cavity, in described cavity, be provided with the pull bar passage passing for pull bar, described pull bar one end is fixedly connected with cutting knife body after running through pull bar passage.
3. tissue culture seeding stem segment cutter sweep as claimed in claim 2, is characterized in that, described pull bar passage is downward-sloping setting from outside to inside.
4. tissue culture seeding stem segment cutter sweep as claimed in claim 2, it is characterized in that, described cutting knife body comprises movable plate, adjusting rod, two expansion links, fixed bar, connecting rod, support bar, blade holder and blade, described fixed bar is fixedly connected with support bar is vertical, described support bar is fixedly connected with movable plate, the two ends of described expansion link are fixedly linked with adjusting rod and fixed bar respectively, described two expansion link horizontal symmetrical arrange, between the symmetrical node of described two expansion links, be provided with connecting rod, described connecting rod and blade holder are fixedly linked, in described blade holder, be provided with blade.
5. tissue culture seeding stem segment cutter sweep as claimed in claim 4, it is characterized in that, the two ends of described connecting rod are connected with expansion link by bearing pin, and described blade holder is set to the cuboid with groove, one end opening of described groove, described blade inserts and is arranged in groove along opening.
6. tissue culture seeding stem segment cutter sweep as claimed in claim 4, it is characterized in that, described support bar is set to a rectangular flat plate, in described dull and stereotyped horizontal direction, be provided with a hollow slideway, on described adjusting rod and connecting rod, be provided with projection, described projection is embedded into and in hollow slideway, carries out horizontal slip.
7. tissue culture seeding stem segment cutter sweep as claimed in claim 4, is characterized in that, the top of described adjusting rod is higher than the top edge of movable plate, and the top of described adjusting rod is provided with handle.
8. tissue culture seeding stem segment cutter sweep as claimed in claim 1, is characterized in that, described knife plate device comprises knife plate frame and be arranged on the knife plate on knife plate frame, and described knife plate pulls and pushes and is arranged on knife plate frame.
9. tissue culture seeding stem segment cutter sweep as claimed in claim 1 or 2, is characterized in that, described casing also comprises a dividing plate, and described dividing plate is arranged between the first drawer and the second drawer, and the height of described left boxboard, dividing plate and the second drawer is all lower than the first drawer; Four edges of described the first drawer left plate are symmetrically arranged with pulley, and front side board and the back side panel of described the first drawer are symmetrically arranged with slideway, and described pulley embeds and is arranged in slideway, and the slip by pulley in slideway realizes the movement of left plate.
10. a using method for the tissue culture seeding stem segment cutter sweep as described in claim 1 to 9 any one, step is as follows,
(1) with sterilized tweezers, get the group training seedling with obvious internode and be placed on the first drawer, and make group training seedling near knife plate, the bottom of group training seedling is placed in the top of the second drawer;
(2) slow pushing drawing rod inwards, blade moves towards knife plate direction, when blade approaches group training seedling, stops pushing drawing rod, by the distance between adjusting rod timely adjustment blade, makes blade in suitable position;
(3) pushing drawing rod inwards rapidly, blade is cut into multistage by group training seedling, and the stem section of group training seedling falls in the first drawer, and the bottom of group training seedling falls in the second drawer;
(4) pull out pull bar, repeat above step.
CN201410273140.6A 2014-06-18 2014-06-18 A kind of tissue culture seeding stem segment cutter sweep and application thereof Active CN104029234B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105532456A (en) * 2015-12-18 2016-05-04 江苏强农农业技术服务有限公司 Rice anther culture breeding auxiliary device

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Publication number Priority date Publication date Assignee Title
CN1034500A (en) * 1987-12-08 1989-08-09 三井石油化学工业株式会社 Prepare the method for cultivating inoculum sections for plant tissue
CN2887545Y (en) * 2004-11-01 2007-04-11 孙飞浩 Safe vegetable shredder
US20080264221A1 (en) * 2007-04-25 2008-10-30 Murata Machinery, Ltd. Work sheet processing system
CN101954644A (en) * 2010-08-12 2011-01-26 吴成芳 Vegetable and fruit shredding device
CN201979489U (en) * 2011-01-30 2011-09-21 毕建杰 Shearing and tweezing integral tool for tissue culture
CN102696486A (en) * 2012-06-30 2012-10-03 山东省果树研究所 Plant test-tube plantlet chopper
CN203887898U (en) * 2014-06-18 2014-10-22 山东省果树研究所 Tissue cultured seedling stem cutting device

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1034500A (en) * 1987-12-08 1989-08-09 三井石油化学工业株式会社 Prepare the method for cultivating inoculum sections for plant tissue
CN2887545Y (en) * 2004-11-01 2007-04-11 孙飞浩 Safe vegetable shredder
US20080264221A1 (en) * 2007-04-25 2008-10-30 Murata Machinery, Ltd. Work sheet processing system
CN101954644A (en) * 2010-08-12 2011-01-26 吴成芳 Vegetable and fruit shredding device
CN201979489U (en) * 2011-01-30 2011-09-21 毕建杰 Shearing and tweezing integral tool for tissue culture
CN102696486A (en) * 2012-06-30 2012-10-03 山东省果树研究所 Plant test-tube plantlet chopper
CN203887898U (en) * 2014-06-18 2014-10-22 山东省果树研究所 Tissue cultured seedling stem cutting device

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105532456A (en) * 2015-12-18 2016-05-04 江苏强农农业技术服务有限公司 Rice anther culture breeding auxiliary device

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