CN210148176U

CN210148176U - Explant cutting device among plant tissue culture

Info

Publication number: CN210148176U
Application number: CN201920960126.1U
Authority: CN
Inventors: 杨颂娟; 符广议; 黄真池
Original assignee: Lingnan Normal University
Current assignee: Lingnan Normal University
Priority date: 2019-06-24
Filing date: 2019-06-24
Publication date: 2020-03-17
Anticipated expiration: 2029-06-24

Abstract

The utility model discloses an explant cutting device among plant group banks up with earth. The utility model provides an explant cutting device among plant tissue culture, wherein, includes glass dustcoat, flexible cutting device and objective table, the glass dustcoat covers on the objective table, flexible cutting device one end is passed the top of glass dustcoat gets into the inside of glass dustcoat is located the top of objective table. The utility model has reasonable design and structure, simple operation and neat and pollution-free incision of the explants.

Description

Explant cutting device among plant tissue culture

Technical Field

The utility model relates to a plant tissue culture technical field, more specifically relates to an explant cutting device in plant group banks up with earth.

Background

Plant tissue culture, namely a plant sterile culture technology, also called in vitro culture, is a subject which utilizes isolated organs, tissues or cells of plant bodies and protoplasts to induce callus, adventitious buds and adventitious roots under the artificial conditions of sterility, a proper artificial culture medium, temperature and the like according to the theory that plant cells have totipotency and finally forms complete plants. Mostly manual operation in plant explant preparation process, the use tools are tweezers, scalpel and filter paper, fixed explant of tweezers is held to one hand, and the other hand is held the sword and is cut the explant on the culture dish of putting filter paper, and with leading to the explant can't cut off completely easily because the atress is uneven during manual operation, cuts repeatedly and causes mechanical damage again easily, and the explant if drop can receive the pollution at the mesa when the cutting process simultaneously. The whole process has long operation time, high labor intensity, low operation efficiency and high pollution rate, and in addition, the used tools need to be sterilized repeatedly in the manual operation process, so the operation is troublesome. Chinese patent publication No. CN207172201U, publication No. 4/3/2018/h, the utility model discloses an explant cutting device, this application discloses an explant cutting device, including clamping device and cutting blade, clamping device is used for the fixed explant of centre gripping, and cutting blade rotatably is connected to the adjustment bench, and the feeding through-hole has just been seted up to the clamping device centre gripping department position on the adjustment bench, and its cutting edge side can move to feeding through-hole top after cutting blade rotates, and the adjustment bench is connected to clamping device through upper and lower adjusting device. The method has the disadvantages that when the explant is cut, the explant is not separated from the surrounding environment and is easily polluted, so that the plant tissue culture fails.

SUMMERY OF THE UTILITY MODEL

The utility model overcomes above-mentioned current plant tissue culture explant cutting in-process is easily polluted and the big shortcoming of the operation degree of difficulty, provides a plant tissue culture explant cutting's mechanical device. The utility model discloses can realize that plant tissue culture cuts explant in-process aseptic ization, rapid, low loss to improve the quality of explant.

In order to achieve the above object, the utility model adopts the following technical scheme: the utility model provides an explant cutting device among plant tissue culture, wherein, includes glass dustcoat, flexible cutting device and objective table, the glass dustcoat covers on the objective table, flexible cutting device one end is passed the top of glass dustcoat gets into the inside of glass dustcoat is located the top of objective table. The glass outer cover can cut off external environment when carrying out the explant cutting, reduces the environmental pollution that the explant received, will treat the explant of cutting and put on the objective table, presses flexible cutting device, and flexible cutting device extension cuts the explant on the objective table, presses flexible cutting device once more, and flexible cutting device shortens, and the cutting operation of explant is accomplished.

Furthermore, flexible cutting device includes that the telescopic link is pressed the button, the telescopic link and can dismantle the blade, the telescopic link is pressed the button and is connected the telescopic link top and worn out the glass dustcoat top outside, the blade can be dismantled in the bottom connection of telescopic link. The objective table comprises an objective plate, a driving shaft and a driven shaft, the driving shaft and the driven shaft are placed on the objective plate, the driving shaft and the driven shaft are connected through a PC film, and the end faces of the driving shaft and the driven shaft are respectively provided with a knob. The explants to be cut were placed on PC film with tweezers. After pressing the telescopic link according to the press button, the telescopic link extension leads to the blade whereabouts of telescopic link bottom, and the cutting explant falls and the incision is neat, and the browning that easily appears in the plant tissue culture process and the problem of mechanical damage are avoided to great degree, presses the telescopic link according to the press button once more, and the telescopic link shortens the blade that drives the telescopic link bottom and rises, takes out the explant after the cutting with tweezers and arranges in on the culture medium. The knob on the driving shaft is rotated, the used PC film is transferred into the driving shaft, and the clean PC film on the driven shaft is transferred out, so that the replacement of the PC film is realized, and the next explant cutting operation is continued.

Furthermore, the glass outer cover and the carrying plate are made of high-temperature resistant materials. In the direction perpendicular to the axis of the driving shaft, the lower parts of the glass on the two sides of the glass outer cover are provided with vertical strip-shaped notches, the glass on the top of the glass outer cover is provided with a strip-shaped opening and scales, and the telescopic cutting device is arranged in the strip-shaped opening in a penetrating mode. Before using the device, the whole assembled device needs to be put into an autoclave for sterilization treatment, and a sterile cutting device is obtained. An operator uses a pair of tweezers to put the explant to be cut into the PC film through the vertical strip-shaped notch on one side of the glass outer cover, and the explant after cutting is taken out from the vertical strip-shaped notch on the other side of the glass outer cover through the pair of tweezers after cutting. The top glass of the glass outer cover is provided with a strip-shaped opening and scales so that the telescopic rod can move back and forth, thereby driving the detachable cutter on the telescopic rod to move back and forth, and cutting different parts or lengths of explants placed on the PC film.

Furthermore, the driving shaft and the driven shaft on the loading plate are made of high-temperature-resistant PE materials, the driving shaft and the driven shaft are cylindrical and detachably connected with the loading plate, and the width of the PC film is consistent with the length of the driving shaft/the length of the driven shaft. Before the device is used, an autoclave is required to be put into the device for sterilization, the high-temperature-resistant PE material enables the driving shaft and the driven shaft not to deform in the autoclave, the PC film is rolled into the driving shaft and the driven shaft before the device is used and is fixed on the corresponding position of the object carrying plate, when the explant cutting operation is completed for one time, the PC film is used by a knob on the end face of the rotating driving shaft to be rotated into the driving shaft, the clean PC film of the driven shaft can be rotated out, and the rotation and the replacement of the PC film can be realized by the cylindrical driving shaft and the driven shaft.

Furthermore, the two sides of the loading plate corresponding to the two ends of the driving shaft/the driven shaft are respectively provided with a fixed rod, and the fixed rods are connected with the loading plate through positioning holes. The fixed rod is used for fixing the PC film, and the failure of explant cutting caused by loosening of the PC film in the cutting process is avoided.

The utility model has the advantages that:

the utility model provides a cleaner and low pollution environment through the cooperation of the sterilized glass outer cover and the objective table; through the telescopic rod pressing button, the telescopic rod and the detachable blade, a semi-automatic method is provided, so that the cutting process is simpler and more convenient, the problems that explants are easily polluted, browned and mechanically damaged are reduced to a certain extent, and the method is quicker and more efficient; through the cooperation of the PC film, the driving shaft and the driven shaft, the experimental article can be recycled, and the resource utilization rate is improved.

Drawings

FIG. 1 is a schematic sectional view of the present invention;

FIG. 2 is a right side schematic view of the present invention;

fig. 3 is a schematic view of the bottom view of the present invention.

In FIGS. 1-2: the device comprises a glass outer cover 1, a telescopic rod pressing button 2, a telescopic rod 3, a detachable blade 4, a loading plate 5, a fixed rod 6, a PC film 7, a driving shaft 8, a driven shaft 9 and a knob 10.

Detailed Description

The present invention will be further described with reference to the accompanying drawings and the following detailed description. Wherein the showings are for the purpose of illustration only and are shown by way of illustration only and not in actual form, and are not to be construed as limiting the present patent; for a better understanding of the embodiments of the present invention, some parts of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product.

As shown in fig. 1 to 3, an explant cutting device in plant tissue culture comprises a glass outer cover 1, a telescopic cutting device and a stage, wherein the glass outer cover 1 covers the stage, and one end of the telescopic cutting device penetrates through the top of the glass outer cover 1 to enter the inside of the glass outer cover 1 and is positioned above the stage. High temperature resistant glass material is adopted all around and at the top of glass dustcoat 1, forms a cuboid type independent space, and the bottom does not have the glass cover, and there is the bar opening and has the scale at glass dustcoat 1 top. The telescopic cutting device comprises a telescopic rod pressing button 2, a telescopic rod 3 and a detachable blade 4, wherein the telescopic rod pressing button 2 is connected with the top end of the telescopic rod 3 and penetrates out of the outer side of the top of the glass outer cover 1, and the detachable blade 4 is connected with the bottom end of the telescopic rod 3. The objective table is including carrying thing board 5, driving shaft 8, driven shaft 9, and glass dustcoat 1 covers on carrying thing board 5, places driving shaft 8 and driven shaft 9 on carrying thing board 5, and driving shaft 8 and driven shaft 9 are connected through PC film 7, and on rolling into driving shaft 8 and driven shaft 9 with PC film 7 before the use, fix on carrying thing board 5, the terminal surface of driving shaft 8 and driven shaft 9 sets up a knob 10 respectively. In the direction perpendicular to the axis of the driving shaft 8, the lower parts of the glass at the two sides of the glass outer cover 1 are provided with vertical strip-shaped gaps, the tweezers can extend into the gaps and rotate around, the two sides of the object carrying plate 5 corresponding to the two ends of the driving shaft 8/the driven shaft 9 are provided with fixing rods 6, and the fixing rods 6 are connected with the object carrying plate 5 through positioning holes.

The working principle of the embodiment is as follows: before using, be drawn into PC film 7 on driving shaft 8 and driven shaft 9, fix on carrying thing board 5, it is assembled to carry thing board 5 and glass dustcoat 1, external environment can be kept apart to glass dustcoat 1 when the cutting with the explant, put into PC film 7 through the vertical bar breach of glass dustcoat 1 one side with tweezers with the explant that will wait to cut, press telescopic link press button 2 to make 3 extensions of telescopic link drive can dismantle blade 4 whereabouts and accomplish the explant cutting rapidly, press telescopic link press button 2 once more and make telescopic link 3 shorten and drive can dismantle blade 4 and rise, take out the explant after the cutting and place in on the culture medium from the vertical bar breach of glass dustcoat 1 opposite side with tweezers. The knob 10 on the driving shaft is rotated, the used PC film 7 is transferred into the driving shaft 8, and the clean PC film 7 on the driven shaft 9 is transferred out, thereby realizing the replacement of the PC film 7 and continuing the next explant cutting operation.

In this embodiment, the operation steps of the cutting device are as follows: firstly, fixing a detachable blade 4 on the tail end of a telescopic rod 3, and fixing a PC film 7 on a driving shaft 8 and a driven shaft 9 and loading the PC film on a loading plate 5; secondly, fixing the fixing rod 6 in a metal hole on the carrying plate 5, and placing the glass outer cover 1 on the carrying plate 5; step three, wrapping the device with kraft paper, putting the wrapped device into an autoclave, sterilizing the device for 20 minutes at 120 ℃, taking the device out, and placing the device on a clean bench; fourthly, on a superclean workbench, clamping explants on a PC film 7 through a vertical strip-shaped notch on one side of a glass outer cover 1 by using tweezers during cutting, placing a plurality of explants once, moving a telescopic rod 3 to a proper position, pressing a first telescopic rod pressing button 2, driving a detachable blade 4 to descend by using the telescopic rod 3, rapidly completing explant cutting, pressing a second telescopic rod pressing button 2, driving the detachable blade 4 to ascend by using the telescopic rod 3, performing multiple cutting on the explants, and taking out the explants through the vertical strip-shaped notch on the other side of the glass outer cover 1 by using the tweezers to place the explants on a culture medium after the explants with uniform length are cut; fifthly, rotating the knob 10 after cutting to replace the PC film 7 and continuing the next cutting operation; and sixthly, after the detachable blade 4, the driving shaft 8 and the driven shaft 9 are detached.

It is obvious that the above embodiments of the present invention are only examples for clearly illustrating the present invention, and are not limitations to the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.

Claims

1. The utility model provides an explant cutting device among plant tissue culture which characterized in that: including the glass dustcoat, flexible cutting device and objective table, the glass dustcoat covers on the objective table, flexible cutting device one end is passed the top of glass dustcoat gets into the inside of glass dustcoat is located the top of objective table.

2. The explant cutting device in plant tissue culture according to claim 1, wherein: the telescopic cutting device comprises a telescopic rod pressing button, a telescopic rod and a detachable blade, wherein the telescopic rod pressing button is connected with the top end of the telescopic rod and penetrates out of the outer side of the top of the glass outer cover, and the detachable blade is connected with the bottom end of the telescopic rod.

3. The explant cutting device in plant tissue culture according to claim 1, wherein: the objective table comprises an objective plate, a driving shaft and a driven shaft, the driving shaft and the driven shaft are placed on the objective plate, the driving shaft and the driven shaft are connected through a PC film, and the end faces of the driving shaft and the driven shaft are respectively provided with a knob.

4. The explant cutting device in plant tissue culture according to claim 3, wherein: the glass outer cover and the carrying plate are made of high-temperature resistant materials.

5. The explant cutting device in plant tissue culture according to claim 3, wherein: in the direction perpendicular to the axis of the driving shaft, the lower parts of the glass on the two sides of the glass outer cover are provided with vertical strip-shaped notches, the glass on the top of the glass outer cover is provided with a strip-shaped opening and scales, and the telescopic cutting device is arranged in the strip-shaped opening in a penetrating mode.

6. The explant cutting device in plant tissue culture according to claim 3, wherein: the driving shaft and the driven shaft on the loading plate are made of high-temperature-resistant PE materials, and are cylindrical and detachably connected with the loading plate.

7. The explant cutting device in plant tissue culture according to claim 3, wherein: the width of the PC film is consistent with the length of the driving shaft/the driven shaft.

8. The explant cutting device in plant tissue culture according to claim 3, wherein: the two sides of the loading plate corresponding to the two ends of the driving shaft/the driven shaft are respectively provided with a fixed rod, and the fixed rods are connected with the loading plate through positioning holes.