CN104017882B - 检测奶牛子宫内膜细胞炎性反应的荧光定量pcr试剂盒及其检测方法和应用 - Google Patents
检测奶牛子宫内膜细胞炎性反应的荧光定量pcr试剂盒及其检测方法和应用 Download PDFInfo
- Publication number
- CN104017882B CN104017882B CN201410270225.9A CN201410270225A CN104017882B CN 104017882 B CN104017882 B CN 104017882B CN 201410270225 A CN201410270225 A CN 201410270225A CN 104017882 B CN104017882 B CN 104017882B
- Authority
- CN
- China
- Prior art keywords
- milk cow
- primer
- pcr
- endometrial cell
- inflammatory reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (2)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410270225.9A CN104017882B (zh) | 2014-06-18 | 2014-06-18 | 检测奶牛子宫内膜细胞炎性反应的荧光定量pcr试剂盒及其检测方法和应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410270225.9A CN104017882B (zh) | 2014-06-18 | 2014-06-18 | 检测奶牛子宫内膜细胞炎性反应的荧光定量pcr试剂盒及其检测方法和应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104017882A CN104017882A (zh) | 2014-09-03 |
CN104017882B true CN104017882B (zh) | 2016-05-04 |
Family
ID=51434876
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201410270225.9A Expired - Fee Related CN104017882B (zh) | 2014-06-18 | 2014-06-18 | 检测奶牛子宫内膜细胞炎性反应的荧光定量pcr试剂盒及其检测方法和应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN104017882B (zh) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104833782B (zh) * | 2015-05-08 | 2016-07-06 | 中国农业科学院兰州畜牧与兽药研究所 | 一种体外筛选和检测抗奶牛子宫内膜炎药物的方法 |
-
2014
- 2014-06-18 CN CN201410270225.9A patent/CN104017882B/zh not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
CN104017882A (zh) | 2014-09-03 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Tajadini et al. | Comparison of SYBR Green and TaqMan methods in quantitative real-time polymerase chain reaction analysis of four adenosine receptor subtypes | |
Gomes et al. | Circulating miR-1, miR-133a, and miR-206 levels are increased after a half-marathon run | |
Inaba et al. | Diagnostic accuracy of LAMP versus PCR over the course of SARS-CoV-2 infection | |
Clausen et al. | Improvement in fetal DNA extraction from maternal plasma. Evaluation of the NucliSens Magnetic Extraction system and the QIAamp DSP Virus Kit in comparison with the QIAamp DNA Blood Mini Kit | |
CN104232781B (zh) | 一种检测HLA‑B*5801等位基因的TaqMan探针实时荧光PCR方法 | |
Li et al. | Detection of Differentially Expressed MicroRNAs in Rheumatic Heart Disease: miR‐1183 and miR‐1299 as Potential Diagnostic Biomarkers | |
CN103146692A (zh) | 一种用于快速检测华法林个体化用药相关基因snp位点的试剂盒及其检测方法 | |
Ge et al. | A Palm Germ-Radar (PaGeR) for rapid and simple COVID-19 detection by reverse transcription loop-mediated isothermal amplification (RT-LAMP) | |
Chi et al. | CRISPR-Cas14a-integrated strand displacement amplification for rapid and isothermal detection of cholangiocarcinoma associated circulating microRNAs | |
CN102925562A (zh) | 氨基糖苷类药物性耳聋易感基因检测试剂盒及方法 | |
Chorley et al. | Methodological considerations for measuring biofluid-based microRNA biomarkers | |
El-Daly et al. | Circulating microRNAs as reliable tumor biomarkers: Opportunities and Challenges facing Clinical Application | |
ITVT20110002A1 (it) | Metodo di determinazione dell'origine di fluidi o tracce biologiche e kit di reagenti per la loro identificazione in un campione. | |
Chen et al. | A universal platform for one-pot detection of circulating non-coding RNA combining CRISPR-Cas12a and branched rolling circle amplification | |
Mar-Aguilar et al. | Use of serum-circulating miRNA profiling for the identification of breast cancer biomarkers | |
Zhao et al. | Identification of miR-4644 as a suitable endogenous normalizer for circulating miRNA quantification in hepatocellular carcinoma | |
Huang et al. | Circulating miR155 expression level is positive with blood pressure parameters: Potential markers of target-organ damage | |
CN104017882B (zh) | 检测奶牛子宫内膜细胞炎性反应的荧光定量pcr试剂盒及其检测方法和应用 | |
WO2013179672A1 (ja) | 子宮内膜症の判定方法 | |
CN104531875A (zh) | 一种MyD88基因突变荧光定量PCR检测试剂盒及检测方法 | |
Mousa et al. | Circulating MicroRNAs in Duchenne muscular dystrophy | |
CN104313124A (zh) | 一种检测牛igf-1基因的特异性引物、其荧光定量pcr检测试剂盒及其检测方法和应用 | |
CN103103259A (zh) | 确定核酸样本的两个预定位点是否具有已知突变的方法、试剂盒以及引物 | |
Lejnine et al. | Gene expression analysis in serial liver fine needle aspirates | |
CN103789447B (zh) | 一种5′端tRNA半分子检测方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20170807 Address after: Baiyun District of Guangzhou City, Guangdong province 510000 North Tai Road No. 1633 Guangzhou private science and Technology Park Branch Road No. 6 green Huichuang Plaza 1 25 storey 2515 room Patentee after: Guangzhou peptide Agriculture Technology Co., Ltd. Address before: 730000 Gansu city of Lanzhou province West Lake Qilihe district along the 335 base Patentee before: Lanzhou Inst. of Animal Husbandary & Veterinary Medicine, Chinese Academy of Agr |
|
TR01 | Transfer of patent right | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160504 Termination date: 20190618 |
|
CF01 | Termination of patent right due to non-payment of annual fee |