CN104017830A - Method for increasing yield of ethanol produced from kitchen wastes through fermentation by two-step enzyme method - Google Patents
Method for increasing yield of ethanol produced from kitchen wastes through fermentation by two-step enzyme method Download PDFInfo
- Publication number
- CN104017830A CN104017830A CN201410247014.3A CN201410247014A CN104017830A CN 104017830 A CN104017830 A CN 104017830A CN 201410247014 A CN201410247014 A CN 201410247014A CN 104017830 A CN104017830 A CN 104017830A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- ethanol
- kitchen wastes
- add
- food waste
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a method for increasing the yield of ethanol produced from kitchen wastes through fermentation by a two-step enzyme method, belonging to the technical field of the chemical industry. The method comprises the following steps: a. preliminarily sorting the kitchen wastes, adding 0.5-1 times of water according to the weight ratio and grinding the kitchen wastes into slurry; b. adding 0.001-0.003 times of acid proteinase and 0.002-0.005 times of pectinase to the slurry according to the weight ratio, preserving heat of the materials at 45-55 DEG C for 1-1.5 hours and then cooling the materials to 28-35 DEG C; and c. then adding 0.002-0.004 times of glucoamylase and inoculating saccharomyces cerevisiae to carry out ethanol fermentation. The method has the beneficial effects that by adding acid proteinase, pectinase and glucoamylase by a two-step method, the use ratios of the nutrient substances are increased, the wort viscosity is reduced, the fermentation period can be shortened by 4-6 hours and the ethanol yield can be increased by 1-4%.
Description
Technical field
the invention belongs to chemical technology field.
Background technology
since 20 century 70s break out global energy crisis, the development and utilization of biomass energy has caused people's attention again, and wherein the production of alcohol fuel is particularly noticeable.China's alcohol production, mainly taking the food crop such as corn, wheat as raw material, from the viewpoint of production cost and resource reutilization two, utilizes the waste of rich in starch and cellulose family to have more advantage as raw material at present.At present the existing agricultural wastes that utilize carry out the research of alcohol production as maize straw, straw, wheat bran etc., and it is less to utilize changing food waste to produce alcohol fuel research at present.
changing food waste refers to food waste and the remnants of family, school, dining room and catering industry, is the important component part of domestic waste.At present, changing food waste at home most cities exists the problems such as unordered, any disposal of management, and human consumer's food safety and healthy in serious threat.In changing food waste, contain abundant starch, also contain in addition the material such as protein, Mierocrystalline cellulose, these are all the fabulous fermentation raw materials of ethanol.Utilize changing food waste fermentative production of ethanol, can effectively solve the problem of environmental pollution of rubbish, realize its minimizing, innoxious and resource utilization, to expanding alcohol production raw material sources, reduce alcohol production cost and all will there is positive meaning simultaneously.
in kitchen waste ethanol fermentation, owing to containing the material such as protein and pectin substance, cause fermentation broth viscosity large, thalline nutritive deficiency, microorganism growth is slow, causes fermentation period long, and fermenting speed is slow.
Summary of the invention
the object of the invention is: provide a kind of two step enzyme methods to improve the method for changing food waste fermentative production of ethanol, it can accelerate to produce with changing food waste the speed of ethanol, and can improve ethanol production.
method of the present invention is:
1, changing food waste is carried out to preliminary sorting, add by weight 0.5-1 water doubly, be ground into slurries;
2, slurries are added by weight to 0.001-0.003 times of aspartic protease and 0.002-0.005 times of polygalacturonase, 45-55 DEG C of insulation 1-1.5 hour, then cools to 28-35 DEG C;
3, add 0.002-0.004 times of saccharifying enzyme, inoculation yeast saccharomyces cerevisiae carries out ethanol fermentation again.
the invention has the beneficial effects as follows: add aspartic protease, polygalacturonase and saccharifying enzyme by two step method, improved utilization of nutrients, reduced mash viscosity, can shorten fermentation period 4-6 hour, improve ethanol production 1-4%.
Embodiment
embodiment 1
(1) get double centner changing food waste, after preliminary sorting, add the water of 0.6 times, be ground into slurries.(2) add 0.15 kilogram of proteolytic enzyme and 0.3 kilograms of pectin enzyme, 50 DEG C keep 1 hour.(3) add 0.3 kilogram of saccharifying enzyme, 30 DEG C of inoculation yeast saccharomyces cerevisiaes carry out ethanol fermentation.Produce 7 kilograms of ethanol.
embodiment 2
(1) get double centner changing food waste (containing more meat or bean product), after preliminary sorting, add the water of 0.8 times, be ground into slurries.(2) add 0.3 kilogram of aspartic protease and 0.3 kilograms of pectin enzyme, 50 DEG C keep 1 hour.(3) add 0.3 kilogram of saccharifying enzyme, 30 DEG C of inoculation yeast saccharomyces cerevisiaes carry out ethanol fermentation.Produce 7.2 kilograms of ethanol.
embodiment 3
(1) get double centner changing food waste (containing more vegetables or fruit), after preliminary sorting, add the water of 0.6 times, be ground into slurries.(2) add 0.2 kilogram of aspartic protease and 0.5 kilograms of pectin enzyme, 50 DEG C keep 1 hour.(3) add 0.2 kilogram of saccharifying enzyme, 30 DEG C of inoculation yeast saccharomyces cerevisiaes carry out ethanol fermentation.Produce 7.1 kilograms of ethanol.
embodiment 4
(1) get double centner changing food waste (containing more rice or noodles), after preliminary sorting, add the water of 1 times, be ground into slurries.(2) add 0.2 kilogram of aspartic protease and 0.3 kilograms of pectin enzyme, 50 DEG C keep 1 hour.(3) add 0.4 kilogram of saccharifying enzyme, 30 DEG C of inoculation yeast saccharomyces cerevisiaes carry out ethanol fermentation.Produce 7.5 kilograms of ethanol.
Claims (1)
1. two step enzyme methods improve a method for changing food waste fermentative production of ethanol, and its method is:
A, changing food waste is carried out to preliminary sorting, add by weight 0.5-1 water doubly, be ground into slurries;
B, slurries are added to 0.001-0.003 times of aspartic protease and 0.002-0.005 times of polygalacturonase by weight, 45-55 DEG C of insulation 1-1.5 hour, then cools to 28-35 DEG C;
C, add 0.002-0.004 times of saccharifying enzyme again, inoculation yeast saccharomyces cerevisiae carries out ethanol fermentation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410247014.3A CN104017830A (en) | 2014-06-06 | 2014-06-06 | Method for increasing yield of ethanol produced from kitchen wastes through fermentation by two-step enzyme method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410247014.3A CN104017830A (en) | 2014-06-06 | 2014-06-06 | Method for increasing yield of ethanol produced from kitchen wastes through fermentation by two-step enzyme method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104017830A true CN104017830A (en) | 2014-09-03 |
Family
ID=51434827
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410247014.3A Pending CN104017830A (en) | 2014-06-06 | 2014-06-06 | Method for increasing yield of ethanol produced from kitchen wastes through fermentation by two-step enzyme method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104017830A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1442483A (en) * | 2003-04-15 | 2003-09-17 | 天津大学 | Method of preparing high concentration ethanol fermentation liquid by paddy rice raw material fermentation |
| CN101033452A (en) * | 2007-02-07 | 2007-09-12 | 北京科技大学 | Composite yeast adapted for kitchen waste ethanol fermentation and application method thereof |
| CN101041849A (en) * | 2007-04-26 | 2007-09-26 | 湖南南方马铃薯产业化工程技术有限公司 | Method for producing fuel ethanol by using potato as raw material |
| CN103571883A (en) * | 2012-07-31 | 2014-02-12 | 青岛嘉能节能环保技术有限公司 | Method for producing fuel ethanol by raw corn fermentation |
| CN103571878A (en) * | 2012-07-31 | 2014-02-12 | 青岛嘉能节能环保技术有限公司 | Composite agent for producing fuel ethanol by jerusalem artichoke powder fermentation |
-
2014
- 2014-06-06 CN CN201410247014.3A patent/CN104017830A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1442483A (en) * | 2003-04-15 | 2003-09-17 | 天津大学 | Method of preparing high concentration ethanol fermentation liquid by paddy rice raw material fermentation |
| CN101033452A (en) * | 2007-02-07 | 2007-09-12 | 北京科技大学 | Composite yeast adapted for kitchen waste ethanol fermentation and application method thereof |
| CN101041849A (en) * | 2007-04-26 | 2007-09-26 | 湖南南方马铃薯产业化工程技术有限公司 | Method for producing fuel ethanol by using potato as raw material |
| CN103571883A (en) * | 2012-07-31 | 2014-02-12 | 青岛嘉能节能环保技术有限公司 | Method for producing fuel ethanol by raw corn fermentation |
| CN103571878A (en) * | 2012-07-31 | 2014-02-12 | 青岛嘉能节能环保技术有限公司 | Composite agent for producing fuel ethanol by jerusalem artichoke powder fermentation |
Non-Patent Citations (2)
| Title |
|---|
| 卢晓霆等: "玉米生料发酵生产燃料乙醇工艺研究", 《中国酿造》 * |
| 晏辉等: "同步糖化发酵从厨余垃圾生产燃料乙醇的研究", 《环境与可持续发展》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102321722B (en) | Method for producing ethanol fuel from kitchen waste | |
| CN101434913B (en) | Wine brewing yeast strain and method for producing ethanol by efficient stalk fermentation | |
| CN102424831B (en) | Method for producing lactic acid by using food waste | |
| CN102972623B (en) | Method for producing protein feed through hypsizygus marmoreus fungus chaff solid state fermentation and protein feed being produced via method for producing protein feed through hypsizygus marmoreus fungus chaff solid state fermentation | |
| CN101851650A (en) | Method for saccharifying cellulose raw material | |
| CN106173193A (en) | A kind of method of solid fermentation bean cake | |
| CN101899398B (en) | Aspergillusniger strain and application thereof | |
| CN103436586B (en) | One utilizes fermentable biomass to produce alcohol technique | |
| CN101575620B (en) | Method for producing fermentation raw material, rice bran oil and protein feed by comprehensively utilizing rice bran | |
| CN105255644A (en) | White spirit distiller grain yeast and white spirit brewing method | |
| CN101914578A (en) | Method for producing ethanol by using citrus peel residue as raw material | |
| CN101617737A (en) | Method for using pineapple peel and slag to prepare high activity dried yeast and ferment feed | |
| CN102703543B (en) | Method for preparing bacterial cellulose by tuberous raw materials | |
| CN104073525A (en) | Method for producing ethanol by virtue of simultaneous saccharification and co-fermentation | |
| CN101565720A (en) | Process for ethanol production from dining and kitchen waste | |
| CN103740675B (en) | A kind of production method of cellulase | |
| CN104498424A (en) | Method for producing mold spore powder by fermentation of crop straws | |
| CN103461654A (en) | Method for producing vinasse protein feed from corn steep liquor | |
| CN103305556B (en) | Method for producing ethanol by fermenting steam explosion quercus acutissima carr | |
| Yao et al. | Relationship between saccharifying capacity and isolation sources for strains of the Rhizopus arrhizus complex | |
| CN103060206B (en) | Fermentation bacteria agent and preparation method and application thereof | |
| CN104017830A (en) | Method for increasing yield of ethanol produced from kitchen wastes through fermentation by two-step enzyme method | |
| CN105062928A (en) | Zymomonas mobilis resistant to high-concentration acetic acid and high-concentration furfural and application thereof | |
| CN104762167A (en) | Mechanized production process for multi-strain yellow wine raw wheat koji | |
| CN104073531A (en) | Method for producing marasmius androsaceus (L. ex Fr.) Fr. extracellular polysaccharide through submerged liquid state fermentation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140903 |