CN103571878A - Composite agent for producing fuel ethanol by jerusalem artichoke powder fermentation - Google Patents
Composite agent for producing fuel ethanol by jerusalem artichoke powder fermentation Download PDFInfo
- Publication number
- CN103571878A CN103571878A CN201210267093.5A CN201210267093A CN103571878A CN 103571878 A CN103571878 A CN 103571878A CN 201210267093 A CN201210267093 A CN 201210267093A CN 103571878 A CN103571878 A CN 103571878A
- Authority
- CN
- China
- Prior art keywords
- parts
- jerusalem artichoke
- high temperature
- fuel ethanol
- beta
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Liquid Carbonaceous Fuels (AREA)
Abstract
The invention relates to a composite agent for producing fuel ethanol by jerusalem artichoke powder fermentation, which comprises 8-10 parts of high temperature-resistant alpha-amylase; a compound enzyme comprising 80-100 parts of glucoamylase, 30-40 parts of pullulanase, 15-20 parts of cellulose, 8-15 parts of acidic protease, 15-25 parts of pectase, 20-30 parts of inulase, 5-10 parts of beta-glucanase, 3-6 parts of beta-mannanase, 5-15 parts of xylanase, and 3-5 parts of phytase; and 80-90 parts of high temperature-resistant dry saccharomyces cerevisiae. The beneficial effects of the invention are that: when the composite agent is used for producing fuel ethanol by jerusalem artichoke powder fermentation, the operation is simple; the stability is good; the fermentation period is short; the conversion efficiency is high; the production cost is low; and certain guiding significance is provided for further realization of jerusalem artichoke fermentation and large-scale fuel ethanol production.
Description
Technical field
The present invention relates to a kind of recombiner for jerusalem artichoke powder producing fuel ethyl alcohol by ferment.
Background technology
Jerusalem artichoke is a kind of per nnial herb, is commonly called as Jerusalem artichoke, Jerusalem artichoke, belongs to composite family Helianthus.Because it has the advantages such as cold-resistant, drought-enduring, Salt And Alkali Tolerance, anti-blown sand, wide adaptability, reproductive ability be strong, make it all have throughout the country distribution.The main component of jerusalem artichoke is polyfructosan, in addition also contains the compositions such as starch, protein, Mierocrystalline cellulose, hemicellulose, pectin substance.These compositions can resolve into and are easy to be utilized by yeast and produce the material of ethanol under the effect of enzyme.Because jerusalem artichoke has the feature of above-mentioned two aspects, become and can replace food crop to produce the very potential energy-source plant that alcohol fuel is used.
Summary of the invention
For a recombiner for jerusalem artichoke powder producing fuel ethyl alcohol by ferment, it includes:
1, high temperature resistant α-amylase 8-10 part;
2, prozyme: saccharifying enzyme 80-100 part, Pullulanase 30-40 part, cellulose enzyme 15-20 part, aspartic protease 8-15 part, polygalacturonase 15-25 part, inulinase 20-30 part, beta-glucanase 5-10 part, 'beta '-mannase 3-6 part, zytase 5-15 part, phytase 3-5 part;
3, high temperature resistant brewer's dried yeasts 80-90 part.
Preferably: the activation method of high temperature resistant brewer's dried yeasts is, take a certain amount of dry yeast in 40-50 doubly in 2% the D/W of 35-40 ℃ of its weight, first in 37 ℃ of thermostat containers, cultivate 15-20min, then transfer in 30 ℃ of thermostat containers and cultivate 60-70 ℃.
The invention has the beneficial effects as follows: adopt this recombiner to carry out fermentation of inulin and produce fuel alcohol; simple to operate, good stability, the advantage such as fermentation period is short, transformation efficiency is high, production cost is low; further realization is utilized to jerusalem artichoke fermentation, and large-scale production alcohol fuel has certain directive significance.
Embodiment
Below in conjunction with embodiment, the present invention will be further described.
Embodiment 1
For a recombiner for jerusalem artichoke powder producing fuel ethyl alcohol by ferment, it includes: 8 parts of high temperature resistant α-amylase, 80 parts, saccharifying enzyme, 35 parts of Pullulanases, 15 parts of cellulose enzymes, 10 parts of aspartic proteases, 15 parts of polygalacturonases, 20 parts of inulinases, 6 parts of beta-glucanases, 3 parts of 'beta '-mannases, 5 parts of zytases, 5 parts of phytases; 80 parts of high temperature resistant brewer's dried yeasts.
During use, first high temperature resistant α-amylase is dissolved in 60 ℃ of water of 20 times of its weight, be cooled to 35 ℃ standby; High temperature resistant brewer's dried yeasts is activated, its method method is again, takes a certain amount of dry yeast in 2% the D/W of 38 ℃ of 40 times of its weight, first in 37 ℃ of thermostat containers, cultivates 15min, then transfers in 30 ℃ of thermostat containers and cultivates 65 ℃; Finally the high temperature resistant α-amylase aqueous solution and yeast glucose solution are mixed, add prozyme, stir and can use.
Embodiment 2
Also formula can be changed into following ratio: 10 parts of high temperature resistant α-amylase, 100 parts, saccharifying enzyme, 35 parts of Pullulanases, 20 parts of cellulose enzymes, 12 parts of aspartic proteases, 22 parts of polygalacturonases, 28 parts of inulinases, 5 parts of beta-glucanases, 6 parts of 'beta '-mannases, 10 parts of zytases, 5 parts of phytases, 85 parts of high temperature resistant brewer's dried yeasts.Other operations are constant.
Claims (2)
1. for a recombiner for jerusalem artichoke powder producing fuel ethyl alcohol by ferment, it includes:
A, high temperature resistant α-amylase 8-10 part;
B, prozyme: saccharifying enzyme 80-100 part, Pullulanase 30-40 part, cellulose enzyme 15-20 part, aspartic protease 8-15 part, polygalacturonase 15-25 part, inulinase 20-30 part, beta-glucanase 5-10 part, 'beta '-mannase 3-6 part, zytase 5-15 part, phytase 3-5 part;
C, high temperature resistant brewer's dried yeasts 80-90 part.
2. according to a kind of method of utilizing the useless grain production feed producing in fuel ethanol production from sweet potato claimed in claim 1, it is characterized in that: the activation method of high temperature resistant brewer's dried yeasts is, take a certain amount of dry yeast in 40-50 doubly in 2% the D/W of 35-40 ℃ of its weight, first in 37 ℃ of thermostat containers, cultivate 15-20 min, then transfer in 30 ℃ of thermostat containers and cultivate 60-70 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210267093.5A CN103571878A (en) | 2012-07-31 | 2012-07-31 | Composite agent for producing fuel ethanol by jerusalem artichoke powder fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210267093.5A CN103571878A (en) | 2012-07-31 | 2012-07-31 | Composite agent for producing fuel ethanol by jerusalem artichoke powder fermentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103571878A true CN103571878A (en) | 2014-02-12 |
Family
ID=50044589
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210267093.5A Pending CN103571878A (en) | 2012-07-31 | 2012-07-31 | Composite agent for producing fuel ethanol by jerusalem artichoke powder fermentation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103571878A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104017830A (en) * | 2014-06-06 | 2014-09-03 | 长春理工大学 | Method for increasing yield of ethanol produced from kitchen wastes through fermentation by two-step enzyme method |
| CN104372032A (en) * | 2014-11-13 | 2015-02-25 | 安徽金种子酒业股份有限公司 | Method for producing fuel ethanol by fermenting wine lees |
| CN105524772A (en) * | 2014-09-29 | 2016-04-27 | 湖南新鸿鹰生物工程有限公司 | Compound ethanol enzyme with acid proteases and method for preparing compound ethanol enzyme |
| WO2020119778A1 (en) * | 2018-12-14 | 2020-06-18 | 南京百斯杰生物工程有限公司 | Use of mannase in fermentation of ethanol |
-
2012
- 2012-07-31 CN CN201210267093.5A patent/CN103571878A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104017830A (en) * | 2014-06-06 | 2014-09-03 | 长春理工大学 | Method for increasing yield of ethanol produced from kitchen wastes through fermentation by two-step enzyme method |
| CN105524772A (en) * | 2014-09-29 | 2016-04-27 | 湖南新鸿鹰生物工程有限公司 | Compound ethanol enzyme with acid proteases and method for preparing compound ethanol enzyme |
| CN104372032A (en) * | 2014-11-13 | 2015-02-25 | 安徽金种子酒业股份有限公司 | Method for producing fuel ethanol by fermenting wine lees |
| CN104372032B (en) * | 2014-11-13 | 2017-12-01 | 安徽金种子酒业股份有限公司 | A kind of method of bent wine grain producing fuel ethyl alcohol by ferment |
| WO2020119778A1 (en) * | 2018-12-14 | 2020-06-18 | 南京百斯杰生物工程有限公司 | Use of mannase in fermentation of ethanol |
| CN111321173A (en) * | 2018-12-14 | 2020-06-23 | 南京百斯杰生物工程有限公司 | Application of mannase in alcohol fermentation |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP1604019B1 (en) | Alcohol product processes | |
| US20060275882A1 (en) | Mash viscosity reduction | |
| A Linares-Pasten et al. | Thermostable glycoside hydrolases in biorefinery technologies | |
| EP2276848B9 (en) | Enhanced fermentation process using molasses | |
| JP2014512828A (en) | Use of cellulase and glucoamylase to increase ethanol yield from fermentation | |
| WO2005121305A1 (en) | Mashing process | |
| AU2009256456B2 (en) | Process for alcohol and co-product production from grain sorghum | |
| EP2279241B1 (en) | Development of strains of the thermotolerant yeast hansenula polymorpha capable of alcoholic fermentation of starch and xylan by expression of starch and xylan degrading enzymes | |
| CN103571878A (en) | Composite agent for producing fuel ethanol by jerusalem artichoke powder fermentation | |
| US20090215127A1 (en) | ph Adjustment Free System For Producing Fermentable Sugars and Alcohol | |
| CN105368878A (en) | Method for producing alcohol by using manihot esculenta crantz as raw material | |
| CN101381665A (en) | Complex enzyme and use thereof | |
| CN102071223B (en) | Compound enzymatic hydrolysis method for straw raw materials | |
| EP1633878A1 (en) | Alcohol product processes | |
| WO2015057517A1 (en) | Use of hemicellulases to improve ethanol production | |
| Zhang et al. | Enzymatic formulation strategies unlock highly-efficient saccharification of distinct pretreated corncobs | |
| CN101376905B (en) | Method for producing fermentable sugar by red ramie bark fibre enzymolysis | |
| CN102559424A (en) | Barley beer brewing method | |
| CN101812492A (en) | Method for degrading cassava starch by pullulanase synergistic dual enzymatic method | |
| CN103627751A (en) | Saccharification method used in fuel ethanol production from cassava straws | |
| Kondo et al. | Applications of yeast cell-surface display in bio-refinery | |
| Sakdaronnarong et al. | Enzyme matching design approach on very high gravity liquefaction and saccharification of cassava root and cassava starch for ethanol fermentation | |
| CN101603056B (en) | Method for fermenting ethanol by utilizing cellulase from animals and microorganisms for cooperating enzymolysis | |
| CN102851325A (en) | Fermentation method for producing ethanol by using enzymatic saccharification of corn cob | |
| US11208670B2 (en) | Method for producing a fermentation product |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140212 |