CN103627751A - Saccharification method used in fuel ethanol production from cassava straws - Google Patents
Saccharification method used in fuel ethanol production from cassava straws Download PDFInfo
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- CN103627751A CN103627751A CN201210296128.8A CN201210296128A CN103627751A CN 103627751 A CN103627751 A CN 103627751A CN 201210296128 A CN201210296128 A CN 201210296128A CN 103627751 A CN103627751 A CN 103627751A
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- saccharification
- cassava
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- cellulase
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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Abstract
The invention discloses a saccharification method used in fuel ethanol production from cassava straws. The saccharification method comprises the following steps of 1, cleaning and air-drying cassava, crushing the dried cassava, screening the cassava powder by a sieve of 60-120 meshes, carrying out sealing and drying, and carrying out preservation for next use, 2, carrying out a hydrolysis process on the cassava powder by an acetic acid/hydrogen peroxide mixed acid having a concentration of 40-60% according to a solid-liquid ratio of 1: 10 to 1: 15 at a hydrolysis temperature of 50 DEG C for 12-24h, and 3, carrying out a saccharification process on the obtained hydrolysate by 200-400U/g of cellulase having a pH value of 4-5 for 36-48h, wherein in the saccharification, one or more auxiliary enzymes such as xylanase, cellobiase and hemicellulase are used and one or more surfactants such as Tweens, Triton X materials and sodium dodecyl benzene sulfonate. The saccharification method adopts an enzyme hydrolysis technology and has the advantages of mild reaction conditions, less by-product, high saccharification yield and small environmental damage.
Description
Technical field
The present invention relates to energy field, a kind of method of saccharification when Cassava stalk is produced alcohol fuel specifically.
Background technology
Energy problem, as a global problem, is more and more subject to common people's extensive concern.The main source of the energy is oil in the world, but oil is nonrenewable resources, and along with the continuous exploitation of oil, the mankind are being faced with the severe challenge of oil and energy dilemma, because oil can proven reserve be about to reach capacity on the earth.In numerous energy, ethanol is a kind of important industrial raw material, be widely used in the fields such as chemical industry, food, drink industry, military project, daily-use chemical industry and medical and health, and ethanol is as a kind of good fuel, can improve fuel qualities.Bio-ethanol can be used as a kind of transport fuel, replaces or partly replaces gasoline, forms a kind of New-type fuel, thereby alleviates to a certain extent the contradiction of oil shortage, and another is convenient, and alcohol fuel does not pollute, environmentally friendly.
Cassava stalk is the wood fibre waste that a class amount is large and concentrated at south China, and the results total area is 43.5 ten thousand km
2, ultimate production 7,300,000 t, produce a large amount of cassava stalks, but shortage is effectively utilized method at present, and the processing mode that employing is arbitrarily abandoned or burned conventionally, causes damage to environment and ecology.Also wasted valuable biomass energy simultaneously.Utilize cassava stalk will produce good environmental and social benefits for waste fuel alcohol.
Due to the complicacy of lignocellulose structure and composition, how by cellulose conversion, to be fermentable reducing sugar effectively, be the key issue that lignocellulose is produced fuel alcohol.At present lignocellulose is converted into sugared method and mainly contains two kinds of acid and enzyme hydrolysis methods.Acid hydrolysis process, reaction conditions is violent, easily generates more inhibition hydrolysis reaction and the by product to the toxic effect of biological fermentation, as furfural, n-Hexadecane, acetic acid etc., and higher to equipment requirements.
Summary of the invention
According to above-mentioned weak point, a kind of method of saccharification while the object of this invention is to provide Cassava stalk production alcohol fuel.
Technical scheme of the present invention is: a kind of method of saccharification when Cassava stalk is produced alcohol fuel, it includes
1, the pre-treatment of cassava: cassava is cleaned, air-dry, pulverize, cross 60-120 mesh sieve hermetically drying and preserve, standby.
2, hydrolysis: adopt acetic acid/hydrogen peroxide mixed-acid hydrolysis, solid-to-liquid ratio is 1:10-1:15, and increasing mixed acid concentration is 40-60%, and hydrolysis temperature is 50 ℃, hydrolysis time 12-24h.
3, saccharification: adopt the cellulase saccharification that is hydrolyzed, cellulase addition is 200-400U/g, and pH is 4-5, reaction times 36-48h.
Preferably: in described saccharifying, add one or more in auxiliary enzymes agent zytase, cellobiase, hemicellulase.
Preferably: in described saccharifying, add one or more in tensio-active agent Tweens, Triton X class or Sodium dodecylbenzene sulfonate.
The invention has the beneficial effects as follows: the present invention adopts enzyme hydrolysis process to there is reaction conditions gentleness, by product is few, saccharification yield is high and to the advantage such as environmental hazard is little.
Embodiment
A method for saccharification when Cassava stalk is produced alcohol fuel, it includes: 1, cassava is cleaned, air-dry, pulverize, cross 60-120 mesh sieve hermetically drying and preserve, standby.2, adopt acetic acid/hydrogen peroxide mixing acid that Tapioca Starch is hydrolyzed, solid-to-liquid ratio is 1:10-1:15, and increasing mixed acid concentration is 40-60%, and hydrolysis temperature is 50 ℃, hydrolysis time 12-24h.3, adopt cellulase to carry out saccharification said hydrolyzed liquid, cellulase addition is 200-400U/g, and pH is 4-5, reaction times 36-48h.Wherein, in saccharifying, add one or more in auxiliary enzymes agent zytase, cellobiase, hemicellulase, also add one or more in tensio-active agent Tweens, Triton X class or Sodium dodecylbenzene sulfonate.
Below in conjunction with specific embodiment, the present invention will be further described.
Embodiment 1
Concrete steps are: 1, cassava cleaned, and air-dry, pulverize, cross 80 mesh sieve hermetically dryings and preserve, standby.2, adopt acetic acid/hydrogen peroxide mixing acid that Tapioca Starch is hydrolyzed, solid-to-liquid ratio is 1:10, and increasing mixed acid concentration is 40%, and hydrolysis temperature is 50 ℃, hydrolysis time 20h.3, adopt cellulase to carry out saccharification said hydrolyzed liquid, cellulase addition is 200U/g, and pH is 4.2, reaction times 36h.Wherein, in saccharifying, add auxiliary enzymes agent cellobiase and hemicellulase, also add tensio-active agent polysorbas20 and Sodium dodecylbenzene sulfonate.
Embodiment 2
Concrete steps are: 1, cassava cleaned, and air-dry, pulverize, cross 100 mesh sieve hermetically dryings and preserve, standby.2, adopt acetic acid/hydrogen peroxide mixing acid that Tapioca Starch is hydrolyzed, solid-to-liquid ratio is 1:15, and increasing mixed acid concentration is 48%, and hydrolysis temperature is 50 ℃, hydrolysis time 24h.3, adopt cellulase to carry out saccharification said hydrolyzed liquid, cellulase addition is 260U/g, and pH is 4.5, reaction times 40h.Wherein, in saccharifying, add auxiliary enzymes agent zytase and hemicellulase, also add tensio-active agent tween 80.
Claims (3)
1. a method for saccharification when Cassava stalk is produced alcohol fuel, it includes
The pre-treatment of A, cassava: cassava is cleaned, air-dry, pulverize, cross 60-120 mesh sieve hermetically drying and preserve, standby;
B, hydrolysis: adopt acetic acid/hydrogen peroxide mixed-acid hydrolysis, solid-to-liquid ratio is 1:10-1:15, and increasing mixed acid concentration is 40-60%, and hydrolysis temperature is 50 ℃, hydrolysis time 12-24h;
C, saccharification: adopt the cellulase saccharification that is hydrolyzed, cellulase addition is 200-400U/g, and pH is 4-5, reaction times 36-48h.
2. the method for saccharification while producing alcohol fuel according to a kind of Cassava stalk claimed in claim 1, is characterized in that: in described saccharifying, add one or more in auxiliary enzymes agent zytase, cellobiase, hemicellulase.
3. the method for saccharification while producing alcohol fuel according to a kind of Cassava stalk claimed in claim 1, is characterized in that: in described saccharifying, add one or more in tensio-active agent Tweens, Triton X class or Sodium dodecylbenzene sulfonate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210296128.8A CN103627751A (en) | 2012-08-20 | 2012-08-20 | Saccharification method used in fuel ethanol production from cassava straws |
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| CN201210296128.8A CN103627751A (en) | 2012-08-20 | 2012-08-20 | Saccharification method used in fuel ethanol production from cassava straws |
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| CN103627751A true CN103627751A (en) | 2014-03-12 |
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| CN201210296128.8A Pending CN103627751A (en) | 2012-08-20 | 2012-08-20 | Saccharification method used in fuel ethanol production from cassava straws |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104073534A (en) * | 2014-07-04 | 2014-10-01 | 天津大学 | Method for reducing dosage of cellulose hydrolase in simultaneous saccharification and cofermentation |
| CN104561129A (en) * | 2014-12-31 | 2015-04-29 | 新乡医学院 | Enzymolysis saccharification processing method of straw |
| CN105603021A (en) * | 2016-03-23 | 2016-05-25 | 河南农业大学 | Method for enhancing tobacco stem enzymolysis effect by using surfactant |
| CN109337947A (en) * | 2018-10-30 | 2019-02-15 | 华南农业大学 | A method of Heating Explosion Sugarcane Bagasse efficiency is improved by alkaline peroxide impregnation and additive |
-
2012
- 2012-08-20 CN CN201210296128.8A patent/CN103627751A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104073534A (en) * | 2014-07-04 | 2014-10-01 | 天津大学 | Method for reducing dosage of cellulose hydrolase in simultaneous saccharification and cofermentation |
| CN104561129A (en) * | 2014-12-31 | 2015-04-29 | 新乡医学院 | Enzymolysis saccharification processing method of straw |
| CN105603021A (en) * | 2016-03-23 | 2016-05-25 | 河南农业大学 | Method for enhancing tobacco stem enzymolysis effect by using surfactant |
| CN105603021B (en) * | 2016-03-23 | 2019-04-09 | 河南农业大学 | A method of cigarette stalk hydrolysis result is improved using surfactant |
| CN109337947A (en) * | 2018-10-30 | 2019-02-15 | 华南农业大学 | A method of Heating Explosion Sugarcane Bagasse efficiency is improved by alkaline peroxide impregnation and additive |
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Application publication date: 20140312 |