CN104013991A - Method for preparing modified regenerated cellulose/alginate hemostatic composite material - Google Patents
Method for preparing modified regenerated cellulose/alginate hemostatic composite material Download PDFInfo
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Abstract
The invention discloses a method for preparing a modified regenerated cellulose/alginate hemostatic composite material, and relates to a method for preparing a hemostatic material, belonging to the technical field of biomedical composite materials. The invention aims to solve the technical problem that an existing alginate material has an unsatisfactory hemostatic effect, poor structure integrity, and relatively low mechanical strength and stability. The method comprises a first step of modification of regenerated cellulose; a second step of preparation of an oxidized regenerated cellulose/sodium alginate water solution; a third step of molding of the hemostatic composite material; and a fourth step of cross-linking and solidification of the hemostatic composite material. According to the modified regenerated cellulose/alginate hemostatic composite material, due to the introduction of a sodium carboxylic acid structure at the C6 site of the regenerated cellulose molecule through the selective oxidization of a TEMPO-NaClO-NaBr oxidation system, the hemostatic property, structure integrity, mechanical strength and stability of the modified regenerated cellulose/alginate hemostatic composite material are improved, and the defects of poor wet strength, low mechanical integrity and easy deformation of ordinary alginate non-woven fabrics are overcome.
Description
Technical field
The present invention relates to a kind of preparation method of hemostatic material, belong to bio-medical composition technical field.
Background technology
Alginic acid is the polysaccharide consisting of mannuronic acid and guluronic acid, has been used as dressing matrix material decades.As dressing substrate, alginic acid is highly biocompatible, can absorb a large amount of wound fluids and form gel; for wound healing provides the healing environment of " moistening ", protection wound surface, reduces wound infection rate; promote wound healing, reduce the discomfort of patient in change dressings process.These superior functions of alginic acid have been brought wide prospect for its application aspect medical dressing.
Yet there is wet strength and mechanical integrity is lower, compliance is poor and anthemorrhagic performance is limited problem in pure alginate sill.Polyblend or compound be the common method addressing these problems.At present, in or the patent of invention of authorizing open at some, used natural or synthesized polymer material, as chitosan (CN1167366 C; CN102824303 A; CN102886070 A; CN100463673 C), collagen protein (CN1167366 C), gelatin (CN102824303 A; CN100463673 C), polyvinyl alcohol (CN101445636 A; CN102274540 A), pectin (CN102274540 A), cellulose fibre (CN102492925 A), fibroin albumen (CN103463668 A), hyaluronic acid (CN103483625 A) etc. carry out the performance of blend or composite modified alginate sill.But these blend above or composite modified material also exist some problems, such as the use of collagen protein and gelatin exists immunity risk; Although chitosan can significantly improve the antibiotic property of material, mechanical strength, anthemorrhagic performance improve limited; Polyvinyl alcohol can improve the mechanical strength of material, but does not significantly improve effect to hemostatic.
Summary of the invention
The object of the invention is that existing alginate material haemostatic effect is undesirable in order to solve, structural intergrity, mechanical strength and the lower technical problem of stability, the preparation method of a kind of modification regeneration cellulose/alginate hemostasis composite is provided.
The preparation method of modification regeneration cellulose/alginate hemostasis composite is carried out according to following steps:
One, regenerated cellulose modification: be 1: 10 by mass ratio 2, 2, 6, the water-soluble mixed liquor that obtains of 6-tetramethyl piperidine-nitrogen-oxide and NaBr, in mixed liquor 2, 2, 6, the gross mass total concentration of 6-tetramethyl piperidine-nitrogen-oxide and NaBr is 0.5～2%, again regenerated celulose fibre knitted fabric is immersed, the pH value of regulator solution is 10～11, isothermal vibration, add NaClO solution, add effective chlorine in NaCLO solution quality be 2, 2, 6, 6-tetramethyl piperidine-nitrogen-oxide and NaBr quality summation 2～4 times, the pH value that drips NaOH solution control reaction system is 10～11, then by the excessive dehydrated alcohol of reactant liquor impouring, then sucking filtration, with dehydrated alcohol, rinse to product and become neutral, then product is inserted to vacuum constant temperature drying baker dry 25～40h at 30～45 ℃, obtain oxidized regenerated cellulose sodium,
Two, oxidized regenerated cellulose sodium/sodium alginate aqueous solution preparation: the ratio that is 1～6: 9 according to mass ratio by oxidized regenerated cellulose sodium and sodium alginate powder is made into the blend solution that oxidized regenerated cellulose sodium and sodium alginate total mass concentration are 1.5～5%, at 30～50 ℃, be stirred to mix homogeneously, obtain oxidized regenerated cellulose sodium/sodium alginate mixed aqueous solution;
Three, hemostasis composite material forming: oxidized regenerated cellulose sodium/sodium alginate mixed aqueous solution is poured in mould,-10 ℃～-20 ℃ pre-freezes 40～20 hours, after pre-freeze completes, mould is placed in to freezer dryer, in temperature, be under-56 ℃～-20 ℃, the vacuum condition that is 10～40Pa, process 20～40h, be dried the rear demoulding, obtain oxidized regenerated cellulose sodium/sodium alginate composite of molding;
Four, hemostasis composite crosslinking curing is processed: it is in 3～15% calcium chloride alcoholic solution that oxidized regenerated cellulose sodium/sodium alginate composite of molding is dipped in to mass concentration, under room temperature, crosslinking curing is 2～8 hours, then in air, naturally dry, obtain modification regeneration cellulose/alginate hemostasis composite.
The present invention can be by controlling structure and the performance of the ratio of oxidized regenerated cellulose sodium/sodium alginate and the total concentration of blend solution regulation and control modification regeneration cellulose/alginate hemostasis composite.Compared with prior art, the beneficial effect of the inventive method:
Modification regeneration cellulose of the present invention/alginate hemostasis composite by the selective oxidation of TEMPO-NaClO-NaBr oxidation system, is introduced carboxylic acid sodium structure on the C6 position of regenerated cellulose molecule, gives oxidized regenerated cellulose sodium water solublity.This water solublity characteristic makes oxidized regenerated cellulose sodium and alginate generation molecular level is compound (participates on the one hand the cross-linking process of alginic acid; Form semi-interpenetrating polymer network structure with alginic acid strand on the other hand), improved structural intergrity, mechanical strength and the stability of gained modification regeneration cellulose/alginate hemostasis composite, overcome that common alginate adhesive-bonded fabric dressing hygrometric state Strong degree is inadequate, mechanical integrity is low, yielding shortcoming.
Oxidized regenerated cellulose sodium in modification regeneration cellulose of the present invention/alginate hemostasis composite, owing to having water solublity feature, therefore, after contacting with hemorrhage wound surface, is met blood and is expanded and dissolve, moisture in absorbing blood rapidly, make blood concentrated, viscosity increases, and velocity of blood flow slows down; After dissolving, the colloid that viscosity is very strong can be formed, and then compressing blood capillary can be stopped up well.On the other hand, the contained Ca of alginate in modification regeneration cellulose of the present invention/alginate hemostasis composite
2+release, can promote the formation of thrombokinase, accelerate hemostasis process.Therefore, modification regeneration cellulose/alginate hemostasis composite has the double-hemostasis function effect of oxidized regenerated cellulose sodium and alginate, bleeding stopping period is shortened greatly, reach the effect of quick-acting haemostatic powder, overcome the shortcoming that common alginate adhesive-bonded fabric dressing hemostatic mechanism is single, anthemorrhagic speed is slow and haemostatic effect is limited.
The preparation process of modification regeneration cellulose of the present invention/alginate hemostasis composite without special installation, reaction condition gentleness, technical maturity, be suitable for the stop blooding large-scale production of composite of modification regeneration cellulose/alginate.
Accompanying drawing explanation
Fig. 1 is the FTIR spectrogram of the oxidized regenerated cellulose sodium of preparation in experiment one;
Fig. 2 is the CP/MAS 13C-NMR wave spectrogram of unmodified regenerated cellulose, oxidized regenerated cellulose sodium in experiment one, in figure, a represents the CP/MAS 13C-NMR wave spectrogram of unmodified regenerated cellulose, and b represents the CP/MAS 13C-NMR wave spectrogram of oxidized regenerated cellulose sodium;
Fig. 3 is the bending digital photograph of modification regeneration cellulose/alginate hemostasis composite of preparation in experiment one.
The specific embodiment
Technical solution of the present invention is not limited to the following cited specific embodiment, also comprises the combination in any between each specific embodiment.
The specific embodiment one: the preparation method of present embodiment modification regeneration cellulose/alginate hemostasis composite is carried out according to following steps:
One, oxidized regenerated cellulose sodium/sodium alginate aqueous solution preparation: the ratio that is 1～6: 9 according to mass ratio by oxidized regenerated cellulose sodium and sodium alginate powder is made into the blend solution that oxidized regenerated cellulose sodium and sodium alginate total mass concentration are 1.5～5%, at 30～50 ℃, be stirred to mix homogeneously, obtain oxidized regenerated cellulose sodium/sodium alginate mixed aqueous solution;
Two, hemostasis composite material forming: oxidized regenerated cellulose sodium/sodium alginate mixed aqueous solution is poured in mould,-10 ℃～-20 ℃ pre-freezes 40～20 hours, after pre-freeze completes, mould is placed in to freezer dryer, in temperature, be under-56 ℃～-20 ℃, the vacuum condition that is 10～40Pa, process 20～40h, be dried the rear demoulding, obtain oxidized regenerated cellulose sodium/sodium alginate composite of molding;
Three, hemostasis composite crosslinking curing is processed: it is in 3～15% calcium chloride alcoholic solution that oxidized regenerated cellulose sodium/sodium alginate composite of molding is dipped in to mass concentration, under room temperature, crosslinking curing is 2～8 hours, then in air, naturally dry, obtain modification regeneration cellulose/alginate hemostasis composite.
The specific embodiment two: present embodiment is different from the specific embodiment one is to be 2: 9 by oxidized regenerated cellulose sodium and sodium alginate powder according to mass ratio in step 1 ratio is made into blend solution.Other is identical with the specific embodiment one.
The specific embodiment three: what present embodiment was different from one of the specific embodiment one or two is to be stirred to mix homogeneously in step 1 at 40 ℃.Other is identical with one of the specific embodiment one or two.
The specific embodiment four: present embodiment is different from one of specific embodiment one to three is-12 ℃～-18 ℃ pre-freezes 38～22 hours in step 2.Other is identical with one of specific embodiment one to three.
The specific embodiment five: present embodiment is different from one of specific embodiment one to four is-15 ℃ of pre-freezes 30 hours in step 2.Other is identical with one of specific embodiment one to four.
The specific embodiment six: present embodiment is different from one of specific embodiment one to five is that the mass concentration of calcium chloride alcoholic solution in step 3 is 10%.Other is identical with one of specific embodiment one to five.
The specific embodiment seven: present embodiment is different from one of specific embodiment one to six is crosslinking curing 5 hours at room temperature in step 3.Other is identical with one of specific embodiment one to six.
Adopt following experimental verification effect of the present invention:
Experiment one:
The preparation method of modification regeneration cellulose/alginate hemostasis composite is carried out according to following steps:
One, regenerated cellulose modification: be 1: 10 by mass ratio 2, 2, 6, the water-soluble mixed liquor that obtains of 6-tetramethyl piperidine-nitrogen-oxide (TEMPO) and NaBr, in mixed liquor 2, 2, 6, the gross mass total concentration of 6-tetramethyl piperidine-nitrogen-oxide and NaBr is 0.5%, again regenerated celulose fibre knitted fabric is immersed, the pH value of regulator solution is 10, isothermal vibration, add NaClO solution, add effective chlorine in NaCLO solution quality be 2, 2, 6, 6-tetramethyl piperidine-nitrogen-oxide and NaBr quality summation 2 times, the pH value that drips NaOH solution control reaction system is 10, then by the excessive dehydrated alcohol of reactant liquor impouring, then sucking filtration, with dehydrated alcohol, rinse to product and become neutral, then product is inserted to vacuum constant temperature drying baker dry 40h at 30 ℃, obtain oxidized regenerated cellulose sodium,
Two, oxidized regenerated cellulose sodium/sodium alginate aqueous solution preparation: 0.8g oxidized regenerated cellulose sodium and 1.2g sodium alginate powder are made into the blend solution that oxidized regenerated cellulose sodium and sodium alginate total mass concentration are 1.5%, at 40 ℃, be stirred to mix homogeneously, obtain oxidized regenerated cellulose sodium/sodium alginate mixed aqueous solution;
Three, hemostasis composite material forming: oxidized regenerated cellulose sodium/sodium alginate mixed aqueous solution is poured in mould,-10 ℃ of pre-freezes 20 hours, after pre-freeze completes, mould is placed in to freezer dryer, under the condition that is 30Pa for-56 ℃, vacuum in temperature, process 40h, be dried the rear demoulding, obtain oxidized regenerated cellulose sodium/sodium alginate composite of molding;
Four, hemostasis composite crosslinking curing is processed: it is in 15% calcium chloride alcoholic solution that oxidized regenerated cellulose sodium/sodium alginate composite of molding is dipped in to mass concentration, under room temperature, crosslinking curing is 2 hours, then in air, naturally dry, obtain modification regeneration cellulose/alginate hemostasis composite (products A).
By Fig. 1, find out oxidized regenerated cellulose sodium salt prepared by TEMPO-NaClO-NaBr oxidation system, at 1415cm
-1there is the medium absworption peak of an intensity at place; At 1605cm
-1there is high-intensity absworption peak at place, and the characteristic peak of the two be-COO-, proves that regenerated cellulose has been modified into oxidized regenerated cellulose sodium salt.
In order to verify the high selectivity of oxidized regenerated cellulose sodium structural generation, to testing the oxidized regenerated cellulose sodium of a preparation, carry out CP/MAS 13C-NMR Spectroscopy Study.Test sample is ground into powder, is encased in ZrO
2in rotor, in upper measure (purchased from Switzerland Bruker company) of nuclear magnetic resonance spectrometer Avance II type (purchased from Switzerland Bruker company).
As shown in Figure 2, compare with unmodified regenerated cellulose, oxidized regenerated cellulose sodium salt sample is at the primary hydroxyl peak intensity of the C-6 position of chemical shift 60ppm because oxidation reduces, and the peak of C-6 position carboxyl carbon appearred in 178ppm place, and the peak position of other position carbon does not change.Therefore, TEMPO-NaClO-NaBr oxidation system, when oxidized regenerated cellulose fiber, is just oxidized to carboxylic acid sodium structure C-6 position primary hydroxyl is high efficiency, and C-2, C-3 position secondary hydroxyl are not oxidized.
With tweezers, press modification regeneration cellulose/alginate hemostasis composite of this experiment preparation and find the soft high resilience of its quality, can arbitrarily bend and do not occur crack, and after folding, can recover (as Fig. 3) arbitrarily.Therefore gained modification regeneration cellulose/alginate hemostasis composite has improved the fragility of pure alginate material, and its mechanical strength can meet instructions for use.
Experiment two:
The preparation method of modification regeneration cellulose/alginate hemostasis composite is carried out according to following steps:
One, regenerated cellulose modification: be 1: 10 by mass ratio 2, 2, 6, 6-tetramethyl piperidine-nitrogen-oxide and NaBr are water-soluble, obtain mixed liquor, in mixed liquor 2, 2, 6, the gross mass total concentration of 6-tetramethyl piperidine-nitrogen-oxide and NaBr is 0.52%, again regenerated celulose fibre knitted fabric is immersed, the pH value of regulator solution is 11, isothermal vibration, add NaClO solution, add effective chlorine in NaCLO solution quality be 2, 2, 6, 6-tetramethyl piperidine-nitrogen-oxide and NaBr quality summation 4 times, the pH value that drips NaOH solution control reaction system is 11, then by the excessive dehydrated alcohol of reactant liquor impouring, then sucking filtration, with dehydrated alcohol, rinse to product and become neutral, then product is inserted to vacuum constant temperature drying baker dry 30h at 35 ℃, obtain oxidized regenerated cellulose sodium,
Two, oxidized regenerated cellulose sodium/sodium alginate aqueous solution preparation: 0.2g oxidized regenerated cellulose sodium and 1.8g sodium alginate powder are made into the blend solution that oxidized regenerated cellulose sodium and sodium alginate total mass concentration are 5%, at 40 ℃, be stirred to mix homogeneously, obtain oxidized regenerated cellulose sodium/sodium alginate mixed aqueous solution;
Three, hemostasis composite material forming: oxidized regenerated cellulose sodium/sodium alginate mixed aqueous solution is poured in mould,-10 ℃ of pre-freezes 20 hours, after pre-freeze completes, mould is placed in to freezer dryer, under the condition that is 30Pa for-56 ℃, vacuum in temperature, process 40h, be dried the rear demoulding, obtain oxidized regenerated cellulose sodium/sodium alginate composite of molding;
Four, hemostasis composite crosslinking curing is processed: it is in 3% calcium chloride alcoholic solution that oxidized regenerated cellulose sodium/sodium alginate composite of molding is dipped in to mass concentration, under room temperature, crosslinking curing is 8 hours, then in air, naturally dry, obtain modification regeneration cellulose/alginate hemostasis composite (product B).
Experiment three:
The preparation method of modification regeneration cellulose/alginate hemostasis composite is carried out according to following steps:
One, regenerated cellulose modification: be 1: 10 by mass ratio 2, 2, 6, the water-soluble mixed liquor that obtains of 6-tetramethyl piperidine-nitrogen-oxide and NaBr, in mixed liquor 2, 2, 6, the gross mass total concentration of 6-tetramethyl piperidine-nitrogen-oxide and NaBr is 1%, again regenerated celulose fibre knitted fabric is immersed, the pH value of regulator solution is 10.5, isothermal vibration, add NaClO solution, add effective chlorine in NaCLO solution quality be 2, 2, 6, 6-tetramethyl piperidine-nitrogen-oxide and NaBr quality summation 3 times, the pH value that drips NaOH solution control reaction system is 10.5, then by the excessive dehydrated alcohol of reactant liquor impouring, then sucking filtration, with dehydrated alcohol, rinse to product and become neutral, then product is inserted to vacuum constant temperature drying baker dry 25h at 40 ℃, obtain oxidized regenerated cellulose sodium,
Two, oxidized regenerated cellulose sodium/sodium alginate aqueous solution preparation: 0.5g oxidized regenerated cellulose sodium and 1.5g sodium alginate powder are made into the blend solution that oxidized regenerated cellulose sodium and sodium alginate total mass concentration are 2.5%, at 40 ℃, be stirred to mix homogeneously, obtain oxidized regenerated cellulose sodium/sodium alginate mixed aqueous solution;
Three, hemostasis composite material forming: oxidized regenerated cellulose sodium/sodium alginate mixed aqueous solution is poured in mould,-10 ℃ of pre-freezes 20 hours, after pre-freeze completes, mould is placed in to freezer dryer, under the condition that is 30Pa for-56 ℃, vacuum in temperature, process 40h, be dried the rear demoulding, obtain oxidized regenerated cellulose sodium/sodium alginate composite of molding;
Four, hemostasis composite crosslinking curing is processed: it is in 10% calcium chloride alcoholic solution that oxidized regenerated cellulose sodium/sodium alginate composite of molding is dipped in to mass concentration, under room temperature, crosslinking curing is 5 hours, then in air, naturally dry, obtain modification regeneration cellulose/alginate hemostasis composite (products C).
By structural intergrity and the mechanical stability of " water suction-dehydration " circulation checking product of the present invention.Material is cut into the sample of 2.0cm * 2.0cm size, is then placed in water and takes out for 30 minutes, with filter paper, blot the water of sample surfaces and weigh.By formula (1), calculate water absorption (WA), each sample is surveyed 5 times, averages as measurement result.Then by water suction after sample drying to constant weight, again test its water absorption, and circulate as stated above 10 times.
M in formula
w---the weight after sample water suction
M
d---dry weight before sample water suction
Table 1 material difference repeats the water absorption data after water suction-dewatering cycle
As shown in Table 1, products A of the present invention, B, C repeat water suction-dewatering cycle 10 these later water absorption decline percentage ratios and are respectively 19%, 16%, 17%, all still keep higher water absorption rate, show that it does not still have avalanche in inner aperture after repeatedly using, so product of the present invention has the beneficial effect that keeps structural integrity, mechanical stability.
Haemostatic effect checking: bleeding stopping period is divided into rabbit ear artery hemostasis model and Hepar Leporis seu Oryctolagi portion hemostasis model two classes, the animal set-up procedure before two class models experiments is all as described below: test material is cut into some of 2.0cm * 2.0cm sizes, weighs, sterilizing, standby.The new zealand rabbit of choosing body weight 3.0-3.5kg is divided into some groups (according to test material kind quantity, determining), and 5 every group, three kinds of products A, B and C that three experiments of contrast the present invention are prepared and the haemostatic effect of commercially available dressing.
Rabbit ear artery hemostasis model: slowly inject after pentobarbital sodium solution anesthetized animal by 40mg/kg dosage vein, by its central arteria auricularis region preserved skin, sterilization, along arteria auricularis direction, cut skin, blunt separation goes out arteria auricularis, vein and nerve, use again scalpel transversely cutting tremulous pulse, after blood is gushed out, with 1 layer of test material, stick in wound surface and use pull and push dynamometer to apply the pressure of 3N immediately, every 10s, observe hemostasis situation, until finally after hemostasis, record bleeding stopping period completely, and take off material and weigh.By formula (2), calculate blood volume, getting 5 cell means is final result.
m
BL＝m
1-m
0 (2)
M in formula
bL---amount of bleeding;
M
1---quality of materials after hemostasis;
M
0---stick front quality of materials.
Hepar Leporis seu Oryctolagi portion hemostasis model: after Animal Anesthesia, its abdominal part is fixing upward, about 5cm * the 4cm of abdominal part preserved skin size, after routine disinfection, under xiphoid-process, along ventrimeson, longitudinally cut skin, blunt separation subcutaneous tissue, exposes peritoneum, longitudinally cuts peritoneum, otch is about 5cm, blots blood and the tissue fluid of cutting part with medical disinfecting gauze.Fully expose liver frontal lobe, on its surface, with scalpel, make the wound of 1.0cm * 1.0cm, the about 3mm of the degree of depth, and by wound surface liver tunicle and under hepatic tissue take off.After blood is gushed out, immediately 1 layer of test material or control sample are sticked in wound surface and use pull and push dynamometer to apply the pressure of 3N, every 10s, observe hemostasis situation, until finally record bleeding stopping period after hemostasis completely, and take off material and weigh, by formula (1), calculate blood volume, getting 5 cell means is final result.
Average bleeding stopping period and the average bleeding of table 2 different materials in different hemostasis model
By table 2 experiment results proved, modification regeneration cellulose prepared by the present invention/alginate hemostasis composite A, B and C are than at least fast 31s of commercially available common gauze bleeding stopping period, than at least fast 22s of commercially available alginate dressing, proof modification regeneration cellulose/alginate hemostasis composite has the double-hemostasis function effect of oxidized regenerated cellulose sodium and alginate, bleeding stopping period is shortened greatly, reach the effect of quick-acting haemostatic powder.
Claims (7)
1. the preparation method of modification regeneration cellulose/alginate hemostasis composite, is characterized in that the preparation method of modification regeneration cellulose/alginate hemostasis composite is carried out according to following steps:
One, oxidized regenerated cellulose sodium/sodium alginate aqueous solution preparation: the ratio that is 1～6: 9 according to mass ratio by oxidized regenerated cellulose sodium and sodium alginate powder is made into the blend solution that oxidized regenerated cellulose sodium and sodium alginate total mass concentration are 1.5～5%, at 30～50 ℃, be stirred to mix homogeneously, obtain oxidized regenerated cellulose sodium/sodium alginate mixed aqueous solution;
Two, hemostasis composite material forming: oxidized regenerated cellulose sodium/sodium alginate mixed aqueous solution is poured in mould,-10 ℃～-20 ℃ pre-freezes 40～20 hours, after pre-freeze completes, mould is placed in to freezer dryer, in temperature, be under-56 ℃～-20 ℃, the vacuum condition that is 10～40Pa, process 20～40h, be dried the rear demoulding, obtain oxidized regenerated cellulose sodium/sodium alginate composite of molding;
Three, hemostasis composite crosslinking curing is processed: it is in 3～15% calcium chloride alcoholic solution that oxidized regenerated cellulose sodium/sodium alginate composite of molding is dipped in to mass concentration, under room temperature, crosslinking curing is 2～8 hours, then in air, naturally dry, obtain modification regeneration cellulose/alginate hemostasis composite.
2. the preparation method of modification regeneration cellulose/alginate hemostasis composite according to claim 1, the ratio that to it is characterized in that in step 1 oxidized regenerated cellulose sodium and sodium alginate powder be 2: 9 according to mass ratio is made into blend solution.
3. the preparation method of modification regeneration cellulose/alginate hemostasis composite according to claim 1, is characterized in that at 40 ℃, being stirred to mix homogeneously in step 1.
4. the preparation method of modification regeneration cellulose/alginate hemostasis composite according to claim 1, is characterized in that in step 2-12 ℃～-18 ℃ pre-freezes 38～22 hours.
5. the preparation method of modification regeneration cellulose/alginate hemostasis composite according to claim 1, is characterized in that in step 2-15 ℃ of pre-freezes 30 hours.
6. the preparation method of modification regeneration cellulose/alginate hemostasis composite according to claim 1, the mass concentration that it is characterized in that calcium chloride alcoholic solution in step 3 is 10%.
7. the preparation method of modification regeneration cellulose/alginate hemostasis composite according to claim 1, is characterized in that in step 3 at room temperature crosslinking curing 5 hours.
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