CN104012946A - Method for microwave hydrolysis of rana japonica oil - Google Patents

Method for microwave hydrolysis of rana japonica oil Download PDF

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Publication number
CN104012946A
CN104012946A CN201310418319.1A CN201310418319A CN104012946A CN 104012946 A CN104012946 A CN 104012946A CN 201310418319 A CN201310418319 A CN 201310418319A CN 104012946 A CN104012946 A CN 104012946A
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China
Prior art keywords
oviductus ranae
microwave
hydrolysis
rana japonica
japonica oil
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Granted
Application number
CN201310418319.1A
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Chinese (zh)
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CN104012946B (en
Inventor
张伟禄
郭峰
钟玲
俞育文
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Wenzhou University
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Wenzhou University
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38—Other non-alcoholic beverages
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/65—Amphibians, e.g. toads, frogs, salamanders or newts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/82—Preparation or application process involves sonication or ultrasonication

Abstract

The invention discloses a method for microwave hydrolysis of rana japonica oil. The method comprises the following steps: (1) washing rana japonica oil, removing most epithelium, adding pure water according to a mass ratio of 1 to (200-800) of rana japonica oil to water, and swelling at 4-25 DEG C for 12-24 hours; (2) homogenizing the swelled rana japonica oil to obtain pulp, subsequently transferring into a microwave reactor, performing a microwave hydrolysis reaction for 30-300 minutes under the conditions that the temperature is 50-200 DEG C and the microwave power is 40-300 W, thereby obtaining primary rana japonica oil hydrolysate; and (3) diluting the primary rana japonica oil hydrolysate according to a mass ratio of 1 to (1-5) of the primary rana japonica oil hydrolysate to pure water, performing suction filtration to remove not depolymerized epithelium, thereby obtaining pure rana japonica oil hydrolysate. The method disclosed by the invention is simple to operate and gentle in condition, the rana japonica oil is utilized to the maximum extent, only pure water is used as a solvent in the whole microwave hydrolysis process, no substances which are unbeneficial for human body health are added, and the pure natural properties of the nutrient components of the rana japonica oil are ensured.

Description

A kind of method of oviductus ranae microwave hydrolysis
(1) technical field
The present invention relates to a kind of method of oviductus ranae hydrolysis, a kind of particularly method of oviductus ranae microwave hydrolysis.
(2) technical background
Oviductus ranae, also claims snow frog oil or OVIDUCTUS RANNAE, is the dry fallopian tubal of female wood frog, is the rare traditional Chinese medicine of China's specialties of the Northeast, has the effect such as tonifying kidney and benefiting sperm, Yin nourishing and lung moistening.In oviductus ranae, be rich in protein, its crude protein content is up to more than 40.0%, and total amino acid content reaches 17.6%.Wherein essential amino acid content reaches 12.8%.In oviductus ranae, also contain various trace elements and the vitamins such as zinc, manganese, copper.Also contain the micro-hormones such as estradiol, progesterone, testosterone.
Be worth although oviductus ranae has high research and development, wherein the content of macro-molecular protein is high, is difficult for being absorbed by the body, and utilization rate is low.Deep development and the utilization of oviductus ranae are greatly limited.Therefore, the new method research of oviductus ranae utilization just seems particularly important, and prospect is also boundless.
Conventionally protein deaggregation is become micromolecular polypeptide or amino acid by people, absorbs to increase it, increases operation rate.Protein in oviductus ranae is main less polypeptide and the amino acid of relative molecular weight that forms after hydrolysis, and absorption rate is higher, and can promote the absorption of the other oroteins in food.Product polypeptide after proteolysis can be dissolved in water (cold water is solubilized also) completely, and solution viscosity is low, also has mobility under 60% high concentration.And the resistance to acids and bases of polypeptid solution is good, heat-resisting quantity is good, and 200 DEG C of heating are also without precipitation.
At present, to the depolymerization research of oviductus ranae, mainly adopt enzymatic isolation method, acid hydrolyzation and alkaline hydrolysis.In enzymatic isolation method, use proteolysis enzyme catalyst, as trypsase, pepsin, papain etc.In acid hydrolyzation, use acid catalyst, example hydrochloric acid, sulfuric acid or nitric acid etc.In alkaline hydrolysis, use base catalyst, as NaOH, potassium hydroxide etc.Although adopt enzymatic isolation method, acid hydrolyzation or alkaline hydrolysis depolymerization OVIDUCTUS RANNAE to have certain effect.But there is certain drawback in the introducing of protease, acid or alkali.Enzymatic isolation method post processing needs the deactivation of enzyme; After acid or alkaline hydrolysis are poly-, need acid or alkali to carry out neutralisation treatment.So these three kinds of depolymerization method environment are unfriendly, and last handling process is loaded down with trivial details, running cost is high.Owing to having increased post-processing step, cause product yield low, be unfavorable for commercialization production.This patent adopts pure water depolymerization, does not add any catalyst, and depolymerization mild condition, and technique is simple, easy to operate.Compare with other depolymerization, microwave pure water depolymerization oviductus ranae environmental protection, especially the method do not affect other composition of oviductus ranae.And the method depolymerization cost is low, is conducive to commercialization and produces.
(3) summary of the invention
The object of this invention is to provide a kind of method of oviductus ranae microwave hydrolysis, the method is simple to operate, mild condition, oviductus ranae has obtained maximum utilization, the whole process of microwave hydrolysis is only with pure water as solvent, do not add anyly to the disadvantageous material of health, ensured the pure natural property of oviductus ranae nutritional labeling.
The technical solution used in the present invention is:
A method for oviductus ranae microwave hydrolysis, is characterized in that described method comprises the following steps:
1) oviductus ranae is cleaned and is removed after most of epithelium, adds pure water, swelling 12-24h at 4-25 DEG C by the mass ratio of oviductus ranae: water=1:200-800;
2) be transferred in microwave reactor after the oviductus ranae homogeneous after swelling is broken into pulpous state, microwave hydrolysis reaction 30-300min under temperature 50-200 DEG C, the condition of microwave power 40-300W, obtains the elementary hydrolyzate of oviductus ranae;
3) press the elementary hydrolyzate of oviductus ranae: the elementary hydrolyzate of pure water=1:1-5 mass ratio dilution oviductus ranae, then suction filtration is removed the epithelium of not depolymerization, obtains pure forest frog thick fat hydrolyzate.
Further, step 2) in, hydrolysising reacting temperature is preferably 70-200 DEG C.
Further, step 2) in, microwave power is preferably 50-200W.
Further, step 2) in, hydrolysis time is 60-240min.
In the present invention, oviductus ranae microwave hydrolysis system is pure aquatic system, in process, do not introduce any material, can be directly by filtration after pure water dilution for product complete depolymerization, filtrate (comprising amino acid and small-molecular peptides more than 20 kinds) can be used to make medical supplies as oral liquid, senior nutrition or beverage, also can be used for preparing cosmetics, as products such as beauty treatment, skin care, whitenings.
Further, described method also comprises the steps: that by the pure forest frog thick fat hydrolyzate of step 3) gained freeze drying obtains oviductus ranae and is hydrolyzed powder after rotary evaporation is concentrated, and powder product is easy to storage and transport.
Compared with prior art, beneficial effect of the present invention is mainly reflected in: the method for oviductus ranae microwave hydrolysis, and simple to operate, mild condition, does not add any catalyst, only under pure water condition, can reach the complete hydrolysis of oviductus ranae by microwave action; The present invention makes the macro-molecular protein in oviductus ranae be converted into the micromolecule polypeptide or the amino acid that more easily absorb, and meanwhile, active ingredient, physiologically active ingredient do not lose, and are conducive to further processing, widen its range of application.
(4) detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described further, but protection scope of the present invention is not limited in this:
Embodiment 1
Get Changbai Mountain, northeast oviductus ranae 10.0g, clean after removing most of epithelium and add pure water by the mass ratio of oviductus ranae: water=1:200, swelling 12h at 4 DEG C.Oviductus ranae after swelling is transferred in microwave reactor after homogenizer homogeneous pulp, and under temperature 70 C, microwave power 50W, microwave hydrolysis reaction 240min, obtains the elementary hydrolyzate of oviductus ranae.Press elementary hydrolyzate: pure water=1:2 mass ratio dilutes elementary oviductus ranae hydrolyzate, and then suction filtration is removed the epithelium of residual not depolymerization, obtains oviductus ranae hydrolyzate, 4 DEG C of preservations of refrigerator.Filtrate sampling is 50mgg by ultraviolet specrophotometer analysis of amino acid concentration -1.
Oviductus ranae hydrolyzate, after rotary evaporation is concentrated, under-20 DEG C of low temperature freezing 24 hours, is then transferred to freeze drier freeze-drying and obtains oviductus ranae hydrolysis powder.
Embodiment 2
Get Changbai Mountain, northeast oviductus ranae 10.0g, clean and remove after most of epithelium, add pure water by the mass ratio of oviductus ranae: water=1:500, swelling 12h at 25 DEG C.Oviductus ranae after swelling is transferred in microwave reactor after homogenizer homogeneous pulp, and 120 DEG C of temperature, under microwave power 70W, microwave hydrolysis reaction 120min, obtains the elementary hydrolyzate of oviductus ranae.Press elementary hydrolyzate: pure water=1:2 mass ratio dilutes elementary oviductus ranae hydrolyzate, and then suction filtration is removed the epithelium of residual not depolymerization, obtains oviductus ranae hydrolyzate, 4 DEG C of preservations of refrigerator.Filtrate sampling is 55mgg with ultraviolet specrophotometer analysis of amino acid content -1.
The preparation of oviductus ranae hydrolysis powder is with embodiment 1.
Embodiment 3
Get Changbai Mountain, northeast oviductus ranae 10.0g, clean and remove after most of epithelium, add pure water by the mass ratio of oviductus ranae: water=1:800, swelling 24h at 25 DEG C.Oviductus ranae after swelling is transferred in microwave reactor after homogenizer homogeneous pulp, and 200 DEG C of temperature, under microwave power 200W, microwave hydrolysis reaction 60min, obtains the elementary hydrolyzate of oviductus ranae.Press elementary hydrolyzate: pure water=1:5 mass ratio dilutes elementary oviductus ranae hydrolyzate, and then suction filtration is removed the epithelium of the residual not depolymerization of small part, obtains oviductus ranae hydrolyzate, 4 DEG C of preservations of refrigerator.Filtrate sampling is 53mgg with ultraviolet specrophotometer analysis of amino acid content -1.
The preparation of oviductus ranae hydrolysis powder is with embodiment 1.

Claims (5)

1. a method for oviductus ranae microwave hydrolysis, is characterized in that described method comprises the following steps:
1) oviductus ranae is cleaned and is removed after most of epithelium, adds pure water, swelling 12-24h at 4-25 DEG C by the mass ratio of oviductus ranae: water=1:200-800;
2) be transferred in microwave reactor after the oviductus ranae homogeneous after swelling is broken into pulpous state, microwave hydrolysis reaction 30-300min under temperature 50-200 DEG C, the condition of microwave power 40-300W, obtains the elementary hydrolyzate of oviductus ranae;
3) press the elementary hydrolyzate of oviductus ranae: the elementary hydrolyzate of pure water=1:1-5 mass ratio dilution oviductus ranae, then suction filtration is removed not after depolymerization epithelium, obtains pure forest frog thick fat hydrolyzate.
2. the method for oviductus ranae microwave hydrolysis as claimed in claim 1, is characterized in that step 2) in, hydrolysising reacting temperature is 70-200 DEG C.
3. the method for oviductus ranae microwave hydrolysis as claimed in claim 2, is characterized in that step 2) in, microwave power is 50-200W.
4. the method for oviductus ranae microwave hydrolysis as claimed in claim 3, is characterized in that step 2) in, hydrolysis time is 60-240min.
5. the method for the oviductus ranae microwave hydrolysis as described in one of claim 1～4, is characterized in that described method also comprises the steps: the pure forest frog thick fat hydrolyzate of step 3) gained after rotary evaporation is concentrated, and freeze drying obtains oviductus ranae hydrolysis powder.
CN201310418319.1A 2013-09-13 2013-09-13 A kind of method of oviductus ranae microwave hydrolysis Expired - Fee Related CN104012946B (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1363239A (en) * 2001-01-03 2002-08-14 李清智 Freeze-dried instant forest frog fat and its preparing process
CN1367165A (en) * 2002-01-18 2002-09-04 黄磊 Method for producing deep-sea amino acid by using fish juice
US20060159810A1 (en) * 2005-01-20 2006-07-20 Marialuci Almeida Method for producing a food product
CN101331943A (en) * 2007-06-29 2008-12-31 丹东科健食品有限公司 Production method of freeze-dry instant health wood-frog oil
CN103110095A (en) * 2011-11-16 2013-05-22 刘永生 Method for processing instant wood frog oil cans

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1363239A (en) * 2001-01-03 2002-08-14 李清智 Freeze-dried instant forest frog fat and its preparing process
CN1367165A (en) * 2002-01-18 2002-09-04 黄磊 Method for producing deep-sea amino acid by using fish juice
US20060159810A1 (en) * 2005-01-20 2006-07-20 Marialuci Almeida Method for producing a food product
CN101331943A (en) * 2007-06-29 2008-12-31 丹东科健食品有限公司 Production method of freeze-dry instant health wood-frog oil
CN103110095A (en) * 2011-11-16 2013-05-22 刘永生 Method for processing instant wood frog oil cans

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