CN104003772B - A kind of method accelerating maize straw decomposition - Google Patents
A kind of method accelerating maize straw decomposition Download PDFInfo
- Publication number
- CN104003772B CN104003772B CN201410233987.1A CN201410233987A CN104003772B CN 104003772 B CN104003772 B CN 104003772B CN 201410233987 A CN201410233987 A CN 201410233987A CN 104003772 B CN104003772 B CN 104003772B
- Authority
- CN
- China
- Prior art keywords
- straw
- maize straw
- microbial inoculum
- decomposition
- auxiliary agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000010902 straw Substances 0.000 title claims abstract description 115
- 240000008042 Zea mays Species 0.000 title claims abstract description 75
- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 75
- 238000000034 method Methods 0.000 title claims abstract description 65
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 title claims abstract description 63
- 235000009973 maize Nutrition 0.000 title claims abstract description 63
- 238000000354 decomposition reaction Methods 0.000 title claims abstract description 38
- 239000012752 auxiliary agent Substances 0.000 claims abstract description 34
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 32
- 230000003413 degradative effect Effects 0.000 claims abstract description 31
- 239000002689 soil Substances 0.000 claims abstract description 14
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 12
- 235000005822 corn Nutrition 0.000 claims abstract description 12
- 238000011081 inoculation Methods 0.000 claims abstract description 8
- 230000001580 bacterial effect Effects 0.000 claims description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- 239000000843 powder Substances 0.000 claims description 16
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 10
- 239000000725 suspension Substances 0.000 claims description 7
- ZVZFHCZCIBYFMZ-UHFFFAOYSA-N 6-methylheptoxybenzene Chemical compound CC(C)CCCCCOC1=CC=CC=C1 ZVZFHCZCIBYFMZ-UHFFFAOYSA-N 0.000 claims description 5
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 5
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 claims description 5
- -1 polyoxyethylene Polymers 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 4
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 3
- 239000004202 carbamide Substances 0.000 claims description 3
- 230000009514 concussion Effects 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 230000003068 static effect Effects 0.000 claims description 3
- 230000001133 acceleration Effects 0.000 claims 5
- 108090000790 Enzymes Proteins 0.000 abstract description 24
- 102000004190 Enzymes Human genes 0.000 abstract description 24
- 229940088598 enzyme Drugs 0.000 abstract description 24
- 230000000694 effects Effects 0.000 abstract description 23
- 108010059892 Cellulase Proteins 0.000 abstract description 18
- 229940106157 cellulase Drugs 0.000 abstract description 18
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 abstract description 7
- 229940059442 hemicellulase Drugs 0.000 abstract description 7
- 108010002430 hemicellulase Proteins 0.000 abstract description 7
- 239000002699 waste material Substances 0.000 abstract description 5
- 238000002474 experimental method Methods 0.000 abstract description 2
- 230000000630 rising effect Effects 0.000 abstract description 2
- 230000015556 catabolic process Effects 0.000 description 32
- 238000006731 degradation reaction Methods 0.000 description 32
- 239000003795 chemical substances by application Substances 0.000 description 23
- 238000000855 fermentation Methods 0.000 description 20
- 230000004151 fermentation Effects 0.000 description 20
- 239000007788 liquid Substances 0.000 description 18
- 239000007787 solid Substances 0.000 description 9
- 238000012360 testing method Methods 0.000 description 9
- 241000209094 Oryza Species 0.000 description 7
- 235000007164 Oryza sativa Nutrition 0.000 description 7
- 235000009566 rice Nutrition 0.000 description 7
- 241000985513 Penicillium oxalicum Species 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 238000013329 compounding Methods 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- IQFVPQOLBLOTPF-HKXUKFGYSA-L congo red Chemical compound [Na+].[Na+].C1=CC=CC2=C(N)C(/N=N/C3=CC=C(C=C3)C3=CC=C(C=C3)/N=N/C3=C(C4=CC=CC=C4C(=C3)S([O-])(=O)=O)N)=CC(S([O-])(=O)=O)=C21 IQFVPQOLBLOTPF-HKXUKFGYSA-L 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000000593 degrading effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 238000004088 simulation Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 108010084185 Cellulases Proteins 0.000 description 1
- 102000005575 Cellulases Human genes 0.000 description 1
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 108091023242 Internal transcribed spacer Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 241000371966 Penicillus <bivalve> Species 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- SRBFZHDQGSBBOR-LECHCGJUSA-N alpha-D-xylose Chemical compound O[C@@H]1CO[C@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-LECHCGJUSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- FFBHFFJDDLITSX-UHFFFAOYSA-N benzyl N-[2-hydroxy-4-(3-oxomorpholin-4-yl)phenyl]carbamate Chemical compound OC1=C(NC(=O)OCC2=CC=CC=C2)C=CC(=C1)N1CCOCC1=O FFBHFFJDDLITSX-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 210000000557 podocyte Anatomy 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- LJCNRYVRMXRIQR-OLXYHTOASA-L potassium sodium L-tartrate Chemical compound [Na+].[K+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O LJCNRYVRMXRIQR-OLXYHTOASA-L 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- 235000011006 sodium potassium tartrate Nutrition 0.000 description 1
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000010907 stover Substances 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
- 229960003487 xylose Drugs 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to the field that agricultural crop straw utilizes, disclose a kind of method accelerating maize straw decomposition, comprise: by corn straw smashing, mix with auxiliary agent, being turned over by stalk after inoculation straw degradative microbial inoculum buries in soil, controlling soil moisture content is the 60%-80% of field capacity, at 20-30 DEG C, carry out decomposition.Pot experiment shows, the decomposition effect of the inventive method to maize straw is best, and straw degradative rate reaches 51.9%, improves 14% than the process of straw directly returning to field.TR auxiliary agent significantly can promote straw degradative microbial inoculum cellulase-producing class, the enzyme of its Filter paperlyase (FPA), cellulase activity (CMCase) and hemicellulase (Xylanase) is lived and is all had larger rising, directly promotes the degraded of stalk.The inventive method facilitates the decomposition of maize straw, accelerates the also field process of maize straw, may be used for the trans-utilization of stalk waste, have broad application prospects.
Description
Technical field
The invention belongs to the field that agricultural crop straw utilizes, relate to the technological method of stalk utilization of waste as resource, be specifically related to a kind of method accelerating maize straw decomposition.
Background technology
China is per year over 200000000 tons of maize straws, and owning amount occupies first place in the world.Stalk is many as rural area yule logs or to pile up, in the form impouring environment such as waste burning traditionally, nowadays the domestic haze weather growed in intensity and stalk wantonly burn inseparable contacting.Meanwhile, straw-like materials is owing to being rich in a large amount of organic carbons and the abundant mineral element such as nitrogen, phosphorus, potassium and can as the available stock of a class high-quality.Therefore stalk being carried out efficient recycling not only can decreasing pollution, protection of the environment, and can turn waste into wealth, and realizes Sustainable development.Straw directly returning to field is method easy, the most economic during stalk resource utilizes, from the experience of domestic and international technology popularization and test, maize straw, except improving soil quality, increases outside soil fertility and can also improve crop yield, produce good economic benefit.Research how to use up to date technics make the stalk in also field faster decomposition then become the key of problem.
Also exist in straw directly returning to field process that straw decomposition speed is slow, lignocellulose mass degradation difficult, affect the problems such as succession crop growth, thus limit applying of this technology to a certain extent.These difficult problems can be effectively solved by inoculation straw degradative microbial inoculum, this is because many microorganisms can secrete relevant cellulase, and the lignocellulose material decomposition in stalk is become the soluble sugars such as glucose, wood sugar, cellobiose, thus destroy the surface tissue of stalk, make stover be the material that can be utilized by the microorganism in soil.Therefore the usual decomposition accelerating stalk when maize straw by using straw degradative microbial inoculum.Such as, but also there are some restraining factors in the use of straw degradative microbial inoculum, microbial inoculum, moisture can not fully contact with stalk three, microbial inoculum is difficult to surely grow and infect stalk inside etc. at the wax layer epidermis of stalk.This makes the effect of degradation bacterial agent fully not play, and the decomposition speed of stalk is restricted.
Summary of the invention
1. technical problem
The present invention aims to provide a kind of product enzyme level that effectively can improve degradation bacterial agent, significantly promotes the method for maize straw process, thus accelerates the recycling process of maize straw, guarantees the Sustainable development of agricultural.
2. technical scheme
Accelerate a method for maize straw decomposition, comprising: by corn straw smashing, mix with auxiliary agent, turned over by stalk after inoculation straw degradative microbial inoculum and bury in soil, controlling soil moisture content is the 60%-80% of field capacity, at 20-30 DEG C, carry out decomposition.
Described maize straw is the maize straw section of 1-2cm.
Described auxiliary agent be polyoxyethylene glycol to isooctyl phenyl ether, also known as triton x-100 (TritonX-100 is abbreviated as TR).
Described maize straw and the amount ratio of auxiliary agent are 50 ~ 250:1g/mL, are preferably 250:1g/mL.
The amount ratio of described maize straw and straw degradative microbial inoculum is 1 ~ 5:1g/mL, is preferably 1 ~ 2:1g/mL.
Described straw degradative microbial inoculum is bacterial strain NJGZ-2 spore concentration 10
7the microbial inoculum of cfu/mL.
Bacterial strain NJGZ-2, Classification And Nomenclature is: penicillium oxalicum (Penicilliumoxalicum), China Committee for Culture Collection of Microorganisms's common micro-organisms center (address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City) is preserved in, preserving number CGMCCNO.7527 April 27 in 2013; See application for a patent for invention 201310289313.9, publication number 103667069A.
Described straw degradative microbial inoculum is prepared from by following methods: be seeded to by bacterial strain NJGZ-2 and produce in Spore cultivation base, 25-30 DEG C of static gas wave refrigerator 6-8 days, then add sterilized water concussion, after filtering culture, namely obtain spore suspension, regulate spore suspension miospore concentration to be 10 with sterilized water
7cfu/mL, obtains straw degradative microbial inoculum;
The preparation of described product spore substratum: corn stalk powder 10.0g, peptone 5.0g, yeast powder 2.0g, urea 0.3g, water 1000mL, pH nature, 121 DEG C of autoclaving 20min.
Described corn stalk powder is that maize straw is dried to water content less than 1%, pulverized the rear corn stalk powder crossed 30 mesh sieves and obtain.
3. beneficial effect
Pot experiment shows, the decomposition effect of the inventive method to maize straw is best, straw degradative rate reaches 51.9%, 14% is improve than the process of straw directly returning to field, improve more than 7% than the process of only using straw degradative microbial inoculum, this illustrates that adding TR auxiliary agent has obvious promoter action to NJGZ-2 straw degradative microbial inoculum decomposition maize straw.Auxiliary agent TritonX-100 significantly can promote straw degradative microbial inoculum cellulase-producing class, the enzyme of its Filter paperlyase (FPA), cellulase activity (CMCase) and hemicellulase (Xylanase) is lived and is all had larger rising, directly promotes the degraded of stalk.Fermentation test result shows, auxiliary agent TritonX-100 all can promote straw degradative microbial inoculum decomposition maize straw under 0.1% concentration and 0.5% concentration, the most obvious with the promoter action of 0.1% concentration to straw degradative microbial inoculum decomposition maize straw, liquid fermenting 7 days and solid fermentation 30 days under 30 DEG C of conditions, maize straw degradation rate is respectively 61.7% and 66.8%, improves 23% and 16.8% than only adding straw degradative microbial inoculum.
The inventive method facilitates the decomposition of maize straw, accelerates the also field process of maize straw, may be used for the trans-utilization of stalk waste, have broad application prospects.
Accompanying drawing explanation
Fig. 1 adds the impact of auxiliary agent on degradation bacterial agent filter paper enzyme activity under liquid fermentation condition.
Fig. 2 adds the impact of auxiliary agent on degradation bacterial agent cellulase activity under liquid fermentation condition.
Fig. 3 adds the impact of auxiliary agent on the work of degradation bacterial agent hemicellulase under liquid fermentation condition.
Fig. 4 is the impact of liquid fermenting different treatment on straw decomposition, and a is stalk before process; B remains stalk after control group fermentation; C remains stalk after adding 0.1%TritonX-100 fermentation.
Fig. 5 is the impact of solid fermentation different treatment on straw decomposition, and a is stalk before process; B remains stalk after control group fermentation; C remains stalk after adding 0.1%TritonX-100 fermentation.
Biomaterial preservation information:
NJGZ-2, Classification And Nomenclature is: penicillium oxalicum (Penicilliumoxalicum), is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number CGMCCNO.7527 April 27 in 2013.
Embodiment
Embodiment 1
This degradation bacteria screens to obtain from Pattern of Zijinshan rots leaf soil.Utilize gradient dilution coating method that the suspension of the pedotheque of stepwise dilution is applied to the Congo red flat board of CMC-, cultivate screen fibre element degradation bacteria for 30 DEG C.The Congo red culture medium prescription of above-mentioned CMC-is: Xylo-Mucine (CMC-Na) 10g, (NH
4)
2sO
42g, KH
2pO
41g, MgSO
47H
2o0.5g, Congo red 0.2g, agar 20g, water 1000mL, 121 DEG C of autoclaving 20min.
Select that on the Congo red flat board of CMC-, to produce transparent circle comparatively large and grow bacterial strain purifies and separates faster, then they are seeded to rice straw be sole carbon source rice straw powder substratum (rice straw powder culture medium prescription is: dry pulverize after rice straw powder 10g, KH
2pO
43.0g; NaNO
33.0g; CaCl
20.5g; MgSO
47H
2o0.5g, FeSO
47H
2o0.0075g, MnSO
4h
2o0.0025g, ZnSO
40.002g, CoCl
20.003g, agar 20g, water 1000mL, 121 DEG C of autoclaving 20min; Rice straw powder refers to rice straw oven dry and cross 40 mesh sieves to water content less than 10%, pulverizing) upper cultivation, more each strain growth speed and growing state, finally obtain the fungi NJGZ-2 of energy efficient degradation rice straw.On PDA flat board, be shallow white, profile rule in the middle of initial stage bacterium colony, outward appearance is fine hair shape.The thread intertexture of hyphal cell, tool tabula; Conidiophore does not have a podocyte, and penicillus is symmetric double wheel-type, and it is sharp-pointed that bottle obstructs elongated gradual change; Conidium is oval, in blue-greenish colour.Adopt fungi universal primer ITS1 and ITS4 to carry out pcr amplification, check order after PCR primer being connected pMD-19T carrier, gained sequence, through NCBI comparison, has the similarity of 99% with Penicilliumoxalicumstraina1s2_d38.Choose some bacterial strain drawing system close with it and grow evolutionary tree, and in conjunction with bacterium colony and hypha form feature, identify that this bacterial strain is penicillium oxalicum (Penicilliumoxalicum).
Embodiment 2
Bacterial strain NJGZ-2 is seeded in the triangular flask that 50mL product Spore cultivation base is housed, 30 DEG C of static gas wave refrigerator 7 days, then add 30mL sterilized water and shake 30min on shaking table, after filtering culture with sterile gauze, namely obtain spore suspension, regulate spore suspension miospore concentration to be 10 with sterilized water
7cfu/mL, obtains straw degradative microbial inoculum.
The preparation of described product spore substratum: corn stalk powder 10.0g, peptone 5.0g, yeast powder 2.0g, urea 0.3g, water 1000mL, pH nature, 121 DEG C of autoclaving 20min.
Described corn stalk powder is that maize straw is dried to water content less than 1%, pulverized the rear corn stalk powder crossed 30 mesh sieves and obtain.
Embodiment 3
The inventive method and straw directly returning to field method is compared on the impact of maize straw decomposition by potted plant simulation test.
Auxiliary agent be polyoxyethylene glycol to isooctyl phenyl ether, get 1mL polyoxyethylene glycol to isooctyl phenyl ether, adding water and being mixed with polyoxyethylene glycol to isooctyl phenyl ether concentration is the compounding agent solution of 0.1%.
Potted plant simulation test: maize straw is cut into the maize straw section of 1-2cm, get the 100 order nylon net bags that 10g maize straw puts into 10cm × 15cm, by after the method to set up process of table 1, nylon sack is tightened, bury in the flowerpot that soil is housed, apart from soil table 3cm depths, control 60%-80% that soil moisture content is field capacity (every 100g soil about add water 25mL) in process of the test, at 20-30 DEG C, quiescent culture measures the degradation rate of maize straw for 30 days afterwards.
The mensuration of maize straw degradation rate: get the stalk residuum after process, washes away mycelia on stalk and other solvends with a large amount of clear water, dries to constant weight for 70 DEG C, measures the quality that stalk reduces, calculate its degradation rate (see table 1).
Stalk quality × 100% before degradation rate=(before process the rear stalk residual mass of stalk quality-process)/process.
Table 1 different treatment method is on the impact of maize straw decomposition
Note: different lowercase represents that between process, difference reaches 5% conspicuous level, lower same.
Test-results shows, the decomposition effect of process 1 pair of maize straw is best, its degradation rate is 51.9%, 14% is improve than the process (process 4) of straw directly returning to field, improve more than 7% than the process (process 2) only inoculating degradation bacterial agent, this illustrates that adding TR auxiliary agent has obvious promoter action to microbial inoculum NJGZ-2 decomposition maize straw.
Embodiment 4
The TR auxiliary agent comparing different concns by solid fermentation test on the impact of degradation bacterial agent degrading maize straws and auxiliary agent on the impact of degradation bacterial agent cellulase-producing class.
Solid fermentation is tested: the compounding agent solution being first respectively 0.1% and 0.5% with TR auxiliary agent and water configuration concentration.Maize straw is got 5g and is added in tissue culture bottle after being cut into the segment of 1-2cm, add 20mL compounding agent solution fully mix with maize straw after through 121 DEG C of autoclaving 20min; Inoculation 5mL straw degradative microbial inoculum in tissue culture bottle, 30 DEG C of quiescent culture 30 days.Within 30 days, extract crude enzyme liquid afterwards and measure its filter paper enzyme activity (FPA), cellulase activity (CMCase) and hemicellulase (Xylanase) alive, and measure straw degradative rate (see table 2).
Controlled trial (CK): not add auxiliary agent, only inoculation straw degradative microbial inoculum in contrast, test with solid fermentation by other process.
The preparation of crude enzyme liquid and enzyme activity determination:
Solid fermentation adds 50mL sterilized water in backward tissue culture bottle in 30 days, gets fermented liquid, after the centrifugal 10min of 5000rpm, namely obtain crude enzyme liquid after shaking table concussion 20min.Measure its filter paper enzyme activity (FPA), cellulase activity (CMCase) and half cellulase with DNS reducing sugar method and live (Xylanase).The definition that enzyme is lived is with reference to international standard: under corresponding condition, at 50 DEG C, and in 1min, the enzyme amount of catalytic substrate hydrolysis generation 1 μm of ol resultant is defined as a unit of enzyme activity (U).
The compound method of DNS reagent: 6.3g3,5-dinitrosalicylic acid and 21gNaOH, is added to 500mL containing in the hydrothermal solution of 185g Seignette salt, then adds 5g crystalline phenol and 5g S-WAT, stirring and dissolving.After cooling, adding distil water is settled to 1000mL, stores in brown bottle for subsequent use after one week.
Under table 2 solid fermentation condition, auxiliary agent TR is on the impact of degradation bacterial agent decomposition maize straw
Note: gds represents every gram of dry weight substrate.
Result shows, the maize straw surface after solid fermentation is all subject to destruction (see Fig. 5) in various degree, but the stalk major part in controlled trial is decomposed, and only has a small amount of section shape structure residual; And be broken down into threadiness through the maize straw major part of TR auxiliary agent process, only there is a small amount of fragment to remain.Solid fermentation 30 days under 30 DEG C of conditions, the degradation rate that the maize straw degradation rate of 0.1%TR process reaches 66.8%, 0.5%TR process reaches 60.7%, improves 16.8% and 10.7% respectively than control group.TR auxiliary agent is to promoting that the effect of degradation bacterial agent cellulase-producing class is fairly obvious, the enzyme of its Filter paperlyase (FPA), cellulase (CMCase) and hemicellulase (Xylanase) is lived and is all greatly improved, and the product enzyme effect of 0.1%TR process is better than 0.5%TR process.Add auxiliary agent TR as can be seen here and significantly can promote degradation bacterial agent decomposition maize straw, especially obvious with the promoter action of 0.1%TR process.
Embodiment 5
The TR auxiliary agent comparing different concns by liquid fermenting test on the impact of degradation bacterial agent degrading maize straws and auxiliary agent on the impact of degradation bacterial agent cellulase-producing class.
Liquid fermenting is tested: the compounding agent solution being first respectively 0.1% and 0.5% with TR auxiliary agent and water configuration concentration.Maize straw is got 2g and is added in 250mL triangular flask after being cut into the segment of 1-2cm, add 100mL compounding agent solution fully mix with maize straw after through 121 DEG C of autoclaving 20min; Inoculation 2mL straw degradative microbial inoculum in triangular flask, 30 DEG C, 170rpm cultivates 7 days.Get thick enzymic fermentation liquid every day and measure its filter paper enzyme activity (FPA) (see Fig. 1), cellulase activity (CMCase) (see Fig. 2) and hemicellulase (Xylanase) (see Fig. 3) alive, within 7 days, measure straw degradative rate (see table 3) afterwards.
Controlled trial (CK): not add auxiliary agent, only inoculation straw degradative microbial inoculum in contrast, test with liquid fermenting by other process.
The preparation of crude enzyme liquid and enzyme activity determination: the aseptic condition lower every day same period takes a morsel after the centrifugal 10min of fermentation liquor 5000rpm and namely obtains crude enzyme liquid in triangular flask, and enzyme activity determination method is with embodiment 4.
Under table 3 liquid fermentation condition, auxiliary agent TR is on the impact of degradation bacterial agent decomposition maize straw
Result shows, the stalk surface after liquid fermenting is all subject to destruction (see Fig. 4) in various degree, but the stalk in controlled trial only has small part to be destroyed, and still has many stalk maintaining segment shape structures; And the stalk after the process of TR auxiliary agent is almost all in threadiness, and Mass lost is obvious.Add auxiliary agent TR and significantly can promote degradation bacterial agent decomposition maize straw, liquid fermenting 7 days under 30 DEG C of conditions, the degradation rate of 0.1%TR process reaches 61.7%, the degradation rate of 0.5%TR process reaches 53.7%, 23% and 15% is improve respectively than control group, the most obvious to the promoter action of degradation bacterial agent decomposition maize straw when being 0.1% concentration with TR.TR auxiliary agent is to promoting that the effect of degradation bacterial agent cellulase-producing class is fairly obvious, the filter paper enzyme activity (FPA) of 0.1%TR process becomes continuous ascendant trend in 7 days, maximum value is 42.01U/gds, all has obvious lifting than 0.5%TR process (26.72U/gds) and control group (13.39U/gds); Cellulase activity (CMCase) also presents same variation tendency, the enzyme of 0.1%TR process live the 7th day time reach maximum value (174.3U/gds), improve 35.5U/gds than 0.5%TR process, is 2.5 times of control group (68.28U/gds); The hemicellulase (Xylanase) alive of each process presented ascendant trend faster in 1-5 days, tend to be steady after 5 days, wherein 0.5%TR and control group in first 6 days without significant difference, 533.4U/gds is added than control group (1637.3U/gds) to enzyme work when the 7th day, the enzyme work of 0.1%TR process presented very fast ascendant trend in 7 days, 7 days later half cellulases are lived and are reached 3524U/gds, improve 1.15 times than control group.This illustrates that TR auxiliary agent all can improve the cellulase-producing level of NJGZ-2 degradation bacterial agent when 0.1% concentration and 0.5% concentration, has obvious promoter action to decomposition maize straw, especially remarkable with the cellulase-producing level of 0.1% concentration.
Claims (6)
1. one kind is accelerated the method for maize straw decomposition, it is characterized in that comprising: by corn straw smashing, mix with auxiliary agent, after inoculation straw degradative microbial inoculum, stalk is turned over and bury in soil, controlling soil moisture content is the 60%-80% of field capacity, at 20-30 DEG C, carry out decomposition;
Described auxiliary agent is that polyoxyethylene glycol is to isooctyl phenyl ether; Described straw degradative microbial inoculum is bacterial strain NJGZ-2 spore concentration 10
7the microbial inoculum of cfu/mL;
Described maize straw and the amount ratio of auxiliary agent are 50 ~ 250:1g/mL; The amount ratio of described maize straw and straw degradative microbial inoculum is 1 ~ 5:1g/mL.
2. the method for acceleration maize straw decomposition according to claim 1, is characterized in that described maize straw is the maize straw section of 1-2cm.
3. the method for acceleration maize straw decomposition according to claim 1, is characterized in that the amount ratio of described maize straw and auxiliary agent is 250:1g/mL.
4. the method for acceleration maize straw decomposition according to claim 1, is characterized in that the amount ratio of described maize straw and straw degradative microbial inoculum is 1 ~ 2:1g/mL.
5. the method for acceleration maize straw decomposition according to claim 1, it is characterized in that described straw degradative microbial inoculum is prepared from by following methods: be seeded to by bacterial strain NJGZ-2 and produce in Spore cultivation base, 20-30 DEG C of static gas wave refrigerator 6-8 days, then sterilized water concussion is added, namely obtain spore suspension after filtering culture, regulate spore suspension miospore concentration to be 10 with sterilized water
7cfu/mL, obtains straw degradative microbial inoculum;
Described product spore substratum is: corn stalk powder 10.0g, peptone 5.0g, yeast powder 2.0g, urea 0.3g, water 1000mL, pH nature, 121 DEG C of autoclaving 20min.
6. the method for acceleration maize straw decomposition according to claim 5, is characterized in that described corn stalk powder is that maize straw is dried to water content less than 1%, pulverized the rear corn stalk powder crossed 30 mesh sieves and obtain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410233987.1A CN104003772B (en) | 2014-05-29 | 2014-05-29 | A kind of method accelerating maize straw decomposition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410233987.1A CN104003772B (en) | 2014-05-29 | 2014-05-29 | A kind of method accelerating maize straw decomposition |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104003772A CN104003772A (en) | 2014-08-27 |
| CN104003772B true CN104003772B (en) | 2016-03-02 |
Family
ID=51364711
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410233987.1A Expired - Fee Related CN104003772B (en) | 2014-05-29 | 2014-05-29 | A kind of method accelerating maize straw decomposition |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104003772B (en) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104326836B (en) * | 2014-11-21 | 2017-11-28 | 中国农业科学院农业资源与农业区划研究所 | The inorganic microorganism fertilizer of stalk particle |
| CN104649757B (en) * | 2015-02-04 | 2018-03-13 | 中国烟草总公司广东省公司 | The high carbon-based additive of soil that a kind of cotton stalk that ferments is prepared |
| CN104649760B (en) * | 2015-02-04 | 2018-03-13 | 中国烟草总公司广东省公司 | The high carbon-based additive of soil that a kind of fermented maize stalk is prepared |
| CN104649762B (en) * | 2015-02-04 | 2018-03-13 | 中国烟草总公司广东省公司 | The high carbon-based additive of soil that a kind of fermenting peanut stalk is prepared |
| CN106348798A (en) * | 2016-08-29 | 2017-01-25 | 宿州市埇桥区杨园种植专业合作社 | Method for returning crop stalks to field by microbial fermentation |
| CN107311803A (en) * | 2017-07-04 | 2017-11-03 | 丁广礼 | A kind of fertilizer conservation, the processing method for resisting hardened rice terrace |
| CN108384732B (en) * | 2018-02-09 | 2021-03-02 | 上海海洋大学 | Rhodobacter sphaeroides for reducing trichlorphon toxicity and application thereof |
| CN109348768A (en) * | 2018-10-15 | 2019-02-19 | 李华 | Weeds straw-returning fertilizing soil method |
| CN110759760A (en) * | 2019-11-21 | 2020-02-07 | 苏州农业职业技术学院 | Microbial composite bacterial powder for degrading farmland straws and preparation method thereof |
| CN112964590B (en) * | 2021-02-01 | 2022-10-04 | 黑龙江省农业科学院耕作栽培研究所 | Method for monitoring field rice straw decomposition rate |
| CN116376744B (en) * | 2023-01-05 | 2024-05-14 | 安徽农业大学 | Stalk rot agent and corn stalk turning and returning and decay promoting method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102586122A (en) * | 2012-02-24 | 2012-07-18 | 熊鹏 | Method for primarily screening high-yield cellulase funguses |
| CN102612910A (en) * | 2012-03-20 | 2012-08-01 | 山东省农业科学院农业资源与环境研究所 | Method for fertilizing winter wheat by mixed basal application of nitrogen fertilizer and maize straw |
| CN103160441A (en) * | 2011-12-14 | 2013-06-19 | 河北农业大学 | Preparation process of penicillium oxalicum fermentation liquor and application of penicillium oxalicum fermentation liquor in agriculture |
| CN103667069A (en) * | 2013-07-11 | 2014-03-26 | 南京农业大学 | Agricultural straw degrading fungus penicillium oxalicum NJGZ-2 and fungal agent thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009225792A (en) * | 2008-02-29 | 2009-10-08 | Sachiko Tsugeta | New lactic acid bacterial strain, and use thereof |
| KR101408683B1 (en) * | 2012-08-07 | 2014-06-18 | 효성오앤비 주식회사 | Organic fertilizer containing antifungal valuable microbes as effective ingredient and its preparation method |
-
2014
- 2014-05-29 CN CN201410233987.1A patent/CN104003772B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103160441A (en) * | 2011-12-14 | 2013-06-19 | 河北农业大学 | Preparation process of penicillium oxalicum fermentation liquor and application of penicillium oxalicum fermentation liquor in agriculture |
| CN102586122A (en) * | 2012-02-24 | 2012-07-18 | 熊鹏 | Method for primarily screening high-yield cellulase funguses |
| CN102612910A (en) * | 2012-03-20 | 2012-08-01 | 山东省农业科学院农业资源与环境研究所 | Method for fertilizing winter wheat by mixed basal application of nitrogen fertilizer and maize straw |
| CN103667069A (en) * | 2013-07-11 | 2014-03-26 | 南京农业大学 | Agricultural straw degrading fungus penicillium oxalicum NJGZ-2 and fungal agent thereof |
Non-Patent Citations (1)
| Title |
|---|
| 不同添加剂处理对水稻秸秆腐解效果的影响;翟修彩 等;《中国农业科学》;20121231;第45卷(第12期);第2412-2419页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104003772A (en) | 2014-08-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104003772B (en) | A kind of method accelerating maize straw decomposition | |
| Zheng et al. | Semi-continuous production of high-activity pectinases by immobilized Rhizopus oryzae using tobacco wastewater as substrate and their utilization in the hydrolysis of pectin-containing lignocellulosic biomass at high solid content | |
| CN103642721B (en) | A kind of Straw in-situ decomposition microbial agent and application thereof | |
| CN109486684B (en) | Low-temperature straw degradation fungus JGDW-1 and microbial inoculum and application thereof | |
| CN102765972B (en) | Organic garbage decomposing bacterium agent, as well as preparation method and application of organic garbage composting bacterium agent | |
| CN102533553B (en) | Organic matter decomposition combined fungicide for rapidly degrading rice straws and application method thereof | |
| CN102174423B (en) | Bacillus licheniformis CH15 for degrading straws and bacterial agent thereof | |
| CN105936881A (en) | Bacillus saccharophila used for degrading alginic acid, and application method thereof | |
| CN103667069A (en) | Agricultural straw degrading fungus penicillium oxalicum NJGZ-2 and fungal agent thereof | |
| CN105695367A (en) | Compound fungicide FX for degrading straws and application thereof | |
| CN103160441B (en) | Preparation process of penicillium oxalicum fermentation liquor and application of penicillium oxalicum fermentation liquor in agriculture | |
| CN103739315B (en) | A kind of preparation method of Green microecological compound fertilizer | |
| CN110551640A (en) | Preparation method of composite microbial inoculum capable of efficiently degrading corn straws | |
| CN105802888A (en) | Bacillus subtilis for degrading edible mushroom dreg cellulose and application of bacillus subtilis | |
| CN110734322A (en) | Method for assisting fermentation of crop straws by alginate | |
| CN103739321B (en) | A kind of Green microecological compound fertilizer | |
| CN110923174A (en) | Rapid decomposition microbial inoculum for corn straw basification and application thereof | |
| CN103992958B (en) | One strain rice straw degradative fungi intends healthy and free from worry Trichoderma spp. ZJC-1 and microbial inoculum thereof | |
| CN102174411B (en) | Penicillium ochrochloron Y5 and microbial inoculum thereof for degrading straw | |
| CN103992957B (en) | One strain pear tree branch degradative fungi and microbial inoculum thereof | |
| CN104673675A (en) | Green micro-ecological compound fertilizer and preparation method thereof | |
| CN110396483B (en) | High-temperature straw degradation bacterium B-8, and microbial inoculum and application thereof | |
| CN102174424B (en) | Microbial bacterial agent for degrading straws | |
| CN102173879B (en) | Method for producing biological potassium fertilizer by utilizing cellulose fermented waste mycelium and biogas residue | |
| CN103992956A (en) | Method for preparing trichoderma biocontrol microbial agent by using edible fungus chaff |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160302 |