CN104000739B - A kind of preparation method of the isinglass fibroin albumen composite nano-fiber membrane of load skin-protecting factor - Google Patents
A kind of preparation method of the isinglass fibroin albumen composite nano-fiber membrane of load skin-protecting factor Download PDFInfo
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- CN104000739B CN104000739B CN201410211383.7A CN201410211383A CN104000739B CN 104000739 B CN104000739 B CN 104000739B CN 201410211383 A CN201410211383 A CN 201410211383A CN 104000739 B CN104000739 B CN 104000739B
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- isinglass
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Abstract
The present invention relates to a kind of preparation method of the isinglass fibroin albumen composite nano-fiber membrane of load skin-protecting factor, including：Skin-protecting factor will be added in isinglass aqueous solution, obtain isinglass spinning liquid；The aqueous solution of fibroin albumen and polyethylene glycol oxide PEO is prepared, skin-protecting factor is subsequently adding, is obtained fibroin albumen spinning liquid；Above-mentioned isinglass spinning liquid and fibroin albumen spinning liquid are transferred in two syringes respectively, double needle spinning is carried out, are collected, crosslinking is obtained final product.Gained composite nano-fiber membrane of the invention can load various skin-protecting factors, and stable performance simultaneously is easy to preserve, nurses in skin care or even reparation field has a extensive future.
Description
Technical field
The invention belongs to the preparation field of composite nano-fiber membrane material, more particularly to a kind of fish of load skin-protecting factor is bright
The preparation method of glue-fibroin albumen composite nano-fiber membrane.
Background technology
Woman is the animal liked to be beautiful.At all times, " one is glanced back to Margarita facial film from the Cleopatra using egg white mask
Laugh at hundred and fawn life " highest-ranking imperial concubine Yang, the use of facial film history for a long time, with medical science applied development, this kind of ancient treatment
Mode has become a kind of important means of modern beauty dermatological treatment and skin care nursing.
In recent years, under the trend that skin care nurses back to nature green, natural material is increasingly closed by people
Note and pursue.Gelatin has the filamentary structure and good bio-compatible of uniqueness as a kind of effective green cosmetic material
Property and low antigenicity, are the study hotspots of the cosmetics of super quality and field of biomedical materials.But, every field institute for a long time
Gelatin material is mainly the extraction from the tissue of pig and cattle.Taking place frequently due to bovine spongiform encephalopathy and foot and mouth disease in recent years, consumer
It is unwilling using the commodity with mammal as raw material, therefore the gelatin material used by every field gradually turns to maritime raw material
Move.Research finds that the polypeptide in abyssal fishes gelatin has good anti-skin photoage function and suppresses cell melanin to close
Into function, thus, isinglass has broad application prospects in beauty and skin care or even wound repair field.
Beauty and skin care effect of silkworm silk, early in the Tang Dynasty Sun Simiao《Qianjin Yao Fang》, Song dynasty Wang Huai it is hidden《Taiping Royal Prescriptions》, it is bright
For Li Shizhen (1518-1593 A.D.)《Compendium of Materia Medica》It is on the books etc. in medicine writings.Silkworm silk is natural protein fibre, wherein containing 70% or so
Fibroin, and protein abundance in fibroin, containing 18 kinds of aminoacid, wherein leucine can accelerate the metabolism of cell, serine,
Threonine can delay skin aging, tryptophan, L-Tyrosine can absorb ultraviolet.At present, the silk polypeplide for extracting from silkworm silk, into
For most popular shield product natural additive in the world, it is widely used in advanced skin care product.
Electrostatic spinning (abbreviation electrospinning) is a kind of method for preparing submicron or nanofiber, with it is simple and efficient, into
The advantages of this cheap, structure-controllable.Electro spinning nano fiber diameter is little, specific surface area is big, porosity is high, used as slow releasing carrier of medication
With very superior attribute.In recent years, preparation process is not related to the green electrospinning of any poisonous and harmful substance and receives much concern,
Products therefrom safety non-toxic, more conducively practical application and industrialization.
The content of the invention
The technical problem to be solved is to provide that a kind of isinglass-fibroin albumen of load skin-protecting factor is compound to be received
The preparation method of rice fibrous membrane, the invention reaction condition are gentle, easy to operate；Experimental provision is simple, beneficial to production in enormous quantities；
The nano fibrous membrane good stability for arriving, can be preserved for a long time, and with good skin-care effect.
A kind of preparation method of the isinglass-fibroin albumen composite nano-fiber membrane of load skin-protecting factor of the present invention, bag
Include：
(1) skin-protecting factor will be added in isinglass aqueous solution, obtains isinglass spinning liquid；
(2) aqueous solution of fibroin albumen and polyethylene glycol oxide PEO is prepared, skin-protecting factor is subsequently adding, is obtained fibroin albumen
Spinning liquid；
(3) above-mentioned isinglass spinning liquid and fibroin albumen spinning liquid are transferred in two syringes respectively, carry out crosspointer
Head spinning, collects, and crosslinking obtains final product the isinglass-fibroin albumen composite nano-fiber membrane of load skin-protecting factor.
In the step (1) skin-protecting factor be the whitening class factor, the antioxidation class factor, the one kind in the anti-inflammation factor or
It is several.
The whitening class factor is resveratrol, vitamin C, vitamin B2, vitamin B3, vitamin B7, gallic acid,
One or more in arbutin, kojic acid, Trenaxmine, Azelaic Acid；The antioxidation class factor is thioctic acid, vitamin C, Vitamin E
One or more in TPGS, Edaravone, Semen Vitis viniferae extract, glutathion GSH, nitric oxide, Cornu Cervi Pantotrichum extract；Antibacterial
Anti-inflammatory factors are antibiotic, vitamin B3, vitamin B5, vitamin B6, vitamin B7In one or more.
In the step (1), (2) in deionized water dissolve skin-protecting factor one or more.
In the step (1), in isinglass spinning solution, the mass percent of isinglass is 40-60%.
0.5-5% of the skin-protecting factor for isinglass quality in the step (1).
In the step (2) in fibroin albumen spinning liquid fibroin albumen mass percent be 10-30%, polyethylene glycol oxide
PEO is 5-20% relative to the mass percent of fibroin albumen.
0.5-5% of the skin-protecting factor for fibroin albumen quality in the step (2).
In the step (3) in double needle spinning, the electrospinning processes parameter of isinglass is：Voltage is 8-20kV, propulsion speed
Spend for 0.1-0.3mL/h, it is 15-20cm to receive distance, and temperature is 20-40 DEG C, and humidity is 30-50%；Fibroin albumen spinning
Electrospinning processes parameter is：Voltage is 10-15kV, and fltting speed is 0.3-0.5mL/h, receives distance for 10-20cm, and temperature is
20-40 DEG C, humidity is 30-50%.
It is characterized in that：Cylinder collection is collected as in the step (3), cylinder parameter is 500-2000r.
It is characterized in that：Crosslinking in the step (3) processes 12-24h for 75% alcohol vapours of Jing.
Beneficial effect
(1) present invention is easy to operate, and experimental provision is simple, beneficial to production in enormous quantities；
(2) preparation time of the invention is short and mild condition；
(3) isinglass-fibroin albumen composite nano-fiber membrane of the various skin-protecting factors of load that prepared by the present invention, is capable of achieving
Drug synergism, improves skin effect；
(4) isinglass-fibroin albumen composite nano-fiber membrane of the various skin-protecting factors of load that prepared by the present invention is stablized
Property is good, can preserve for a long time, and with good skin-care effect.
Description of the drawings
Fig. 1 is the scanning electron microscope sem photo of 1 products therefrom of embodiment；
Fig. 2 is the SEM photograph of 2 products therefrom of embodiment；
Fig. 3 is the SEM photograph of 3 products therefrom of embodiment；
Fig. 4 is the common light microscopic photo of 3 products therefrom of embodiment；
Fig. 5 is double needle cospinning schematic diagram.
Specific embodiment
With reference to specific embodiment, the present invention is expanded on further.It should be understood that these embodiments are merely to illustrate the present invention
Rather than limit the scope of the present invention.In addition, it is to be understood that after the content for having read instruction of the present invention, people in the art
Member can be made various changes or modifications to the present invention, and these equivalent form of values equally fall within the application appended claims and limited
Scope.
Embodiment 1
It is loaded with the isinglass-fibroin albumen composite nano fiber facial film of vitamin C, Vitamin E, thioctic acid.
(1) isinglass spinning liquid (60%, mass percent) is prepared, and according to mass ratio 0.5-5% (relative to isinglass
Mass ratio) ratio add vitamin C and Vitamin E；
(2) prepare the fibroin containing 20% fibroin albumen (mass percent) and 10%PEO (relative to the mass ratio of fibroin)
Albumen spinning solution, and the ratio according to mass ratio 0.5-5% adds the third medicine thioctic acid；
(3) step (1) and the spinning liquid in (2) are transferred in two syringes respectively, the voltage for adjusting fibroin is
12kV, propulsion speed are 0.3mL/h, and it is 15cm to receive distance；The voltage for adjusting isinglass is 15kV, and fltting speed is 0.2ml/
H, it is 15cm to receive distance；Temperature is 25 DEG C, is received using cylinder, and load vitamin C, Vitamin E, the fish of thioctic acid is obtained
Gelatin-fibroin albumen composite nano-fiber membrane.
(4) nano fibrous membrane obtained by is processed with 75% alcohol vapour and can use after 24h.
Embodiment 2
It is loaded with Semen Vitis viniferae extract, vitamin B6, kojic acid isinglass-fibroin albumen composite nano fiber facial film.
(1) isinglass spinning liquid (60%, mass percent) is prepared, and according to mass ratio 0.5-5% (relative to isinglass
Mass ratio) ratio add kojic acid；
(2) prepare the fibroin containing 20% fibroin albumen (mass percent) and 10%PEO (relative to the mass ratio of fibroin)
Albumen spinning solution, and the ratio according to mass ratio 0.5-5% adds Semen Vitis viniferae extract and vitamin A；
(3) step (1) and the spinning liquid in (2) are transferred in two syringes respectively, the voltage for adjusting fibroin is
12kV, propulsion speed are 0.3mL/h, and it is 15cm to receive distance；The voltage for adjusting isinglass is 15kV, and fltting speed is 0.2ml/
H, it is 15cm to receive distance；Temperature is 25 DEG C, is received using cylinder, and load Semen Vitis viniferae extract, vitamin B is obtained6, kojic acid
Isinglass-fibroin albumen composite nano-fiber membrane.
(4) nano fibrous membrane obtained by is processed with 75% alcohol vapour and can use after 24h.
Embodiment 3
It is loaded with the isinglass-fibroin albumen composite nano fiber facial film of resveratrol, Cornu Cervi Pantotrichum extract, Azelaic Acid.
(1) isinglass spinning liquid (60%, mass percent) is prepared, and according to mass ratio 0.5-5% (relative to isinglass
Mass ratio) ratio add Fructus Schisandrae Chinensis and Cornu Cervi Pantotrichum extract；
(2) prepare the fibroin containing 20% fibroin albumen (mass percent) and 10%PEO (relative to the mass ratio of fibroin)
Albumen spinning solution, and the ratio according to mass ratio 0.5-5% adds Azelaic Acid；
(3) step (1) and the spinning liquid in (2) are transferred in two syringes respectively, the voltage for adjusting fibroin is
12kV, propulsion speed are 0.3mL/h, and it is 15cm to receive distance；The voltage for adjusting isinglass is 15kV, and fltting speed is 0.2ml/
H, it is 15cm to receive distance；Temperature is 25 DEG C, is received using cylinder, and load resveratrol, Cornu Cervi Pantotrichum extract, Azelaic Acid is obtained
Isinglass-fibroin albumen composite nano-fiber membrane.
(4) nano fibrous membrane obtained by is processed with 75% alcohol vapour and can use after 24h.
Claims (4)
1. it is a kind of load skin-protecting factor isinglass-fibroin albumen composite nano-fiber membrane preparation method, including：
(1) skin-protecting factor will be added in isinglass aqueous solution, obtains isinglass spinning liquid；The matter of isinglass in isinglass spinning liquid
Amount percentage ratio is 40-60%；0.5-5% of the skin-protecting factor for isinglass quality；
(2) aqueous solution of fibroin albumen and polyethylene glycol oxide PEO is prepared, skin-protecting factor is subsequently adding, is obtained fibroin albumen spinning
Liquid；Wherein in fibroin albumen spinning liquid, the mass percent of fibroin albumen is 10-30%, and polyethylene glycol oxide PEO is relative to fibroin
The mass percent of albumen is 5-20%；0.5-5% of the skin-protecting factor for fibroin albumen quality；
(3) above-mentioned isinglass spinning liquid and fibroin albumen spinning liquid are transferred in two syringes respectively, carry out double needle spinning
Silk, collects, and crosslinking obtains final product the isinglass-fibroin albumen composite nano-fiber membrane of load skin-protecting factor；Wherein crosslinking is Jing 75%
Alcohol vapour processes 12-24h.
2. it is according to claim 1 it is a kind of load skin-protecting factor isinglass-fibroin albumen composite nano-fiber membrane system
Preparation Method, it is characterised in that：In the step (1), skin-protecting factor is the whitening class factor, the antioxidation class factor, the anti-inflammation factor
In one or more.
3. it is according to claim 2 it is a kind of load skin-protecting factor isinglass-fibroin albumen composite nano-fiber membrane system
Preparation Method, it is characterised in that：The whitening class factor is resveratrol, vitamin C, vitamin B2, vitamin B3, vitamin B7、
One or more in gallic acid, arbutin, kojic acid, Trenaxmine, Azelaic Acid；The antioxidation class factor is thioctic acid, vitamin
One kind in C, Vitamin E TPGS, Edaravone, Semen Vitis viniferae extract, glutathion GSH, nitric oxide, Cornu Cervi Pantotrichum extract
Or it is several；The anti-inflammation factor is antibiotic, vitamin B3, vitamin B5, vitamin B6, vitamin B7In one or more.
4. it is according to claim 1 it is a kind of load skin-protecting factor isinglass-fibroin albumen composite nano-fiber membrane system
Preparation Method, it is characterised in that：In the step (3) in double needle spinning, the electrospinning processes parameter of isinglass is：Voltage is 8-
20kV, fltting speed are 0.1-0.3mL/h, and it is 15-20cm to receive distance, and temperature is 20-40 DEG C, and humidity is 30-50%；Fibroin
The electrospinning processes parameter of albumen spinning is：Voltage is 10-15kV, and fltting speed is 0.3-0.5mL/h, and it is 10- to receive distance
20cm, temperature are 20-40 DEG C, and humidity is 30-50%.
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| CN201410211383.7A CN104000739B (en) | 2014-05-19 | 2014-05-19 | A kind of preparation method of the isinglass fibroin albumen composite nano-fiber membrane of load skin-protecting factor |
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| CN201410211383.7A CN104000739B (en) | 2014-05-19 | 2014-05-19 | A kind of preparation method of the isinglass fibroin albumen composite nano-fiber membrane of load skin-protecting factor |
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| CN104000739B true CN104000739B (en) | 2017-03-29 |
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| CN104342775A (en) * | 2014-10-15 | 2015-02-11 | 东华大学 | Method for preparing composite nano-fiber membrane with honey and natural materials on basis of environment-friendly electrospinning technology |
| CN105200801B (en) * | 2015-10-21 | 2017-05-10 | 江苏箭鹿毛纺股份有限公司 | Preparation method of skin care fabric capable of caring whole body skin |
| CN105561388B (en) * | 2015-12-24 | 2018-10-23 | 东华大学 | A method of galactolipin chitosan-native protein composite nano fiber biomimetic scaffolds are prepared based on green electrospinning |
| CN105919828B (en) * | 2016-07-07 | 2019-06-21 | 广州莱丽奇生物科技有限公司 | A kind of Whitening, spot composition, preparation method and applications |
| CN106400311A (en) * | 2016-09-07 | 2017-02-15 | 东华大学 | Method for preparing composite nanofiber tissue engineering scaffold based on graphene oxide |
| CN106473942A (en) * | 2016-10-31 | 2017-03-08 | 成都远睿生物技术有限公司 | A kind of anti-ageing face mask and preparation method thereof |
| CN106551806B (en) * | 2016-12-01 | 2019-07-12 | 华南协同创新研究院 | A kind of gradually molten formula moisture saver mask and preparation method thereof |
| CN108785192B (en) * | 2017-11-23 | 2021-06-15 | 杨茉莉 | Mineral oil-free natural beauty skin care product and preparation method thereof |
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| CN109853130A (en) * | 2018-12-21 | 2019-06-07 | 北京工商大学 | The ultra-thin super stemness nano fibrous membrane paper of one kind and preparation method |
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| CN111733471A (en) * | 2020-04-23 | 2020-10-02 | 罗莱生活科技股份有限公司 | Skin care fiber capable of slowly releasing skin care ingredients and preparation method and application thereof |
| CN111793900A (en) * | 2020-06-15 | 2020-10-20 | 中国人民解放军陆军特色医学中心 | Chitosan/polycaprolactone composite nanofiber membrane material and application thereof |
| CN113304329B (en) * | 2021-05-27 | 2022-04-12 | 北京化工大学 | Iodine-containing antibacterial medical operation film and preparation method thereof |
| CN116672275B (en) * | 2023-08-02 | 2023-11-14 | 中国科学院理化技术研究所 | Whitening and antioxidant composition, whitening and antioxidant product prepared from same and preparation method |
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| CN101736430B (en) * | 2010-02-05 | 2012-07-04 | 东华大学 | Method for preparing silk fibroin nano-fibre with skin-care effect |
| CN102560887B (en) * | 2012-01-17 | 2014-01-29 | 东华大学 | Silk fibroin nano-fiber film loaded with vitamins A and E and preparation method thereof |
| CN102936794B (en) * | 2012-11-20 | 2015-01-21 | 东华大学 | Method for preparing composite nanofiber membrane based on natural material silk fibroin and chitosan |
| CN103083224A (en) * | 2013-02-06 | 2013-05-08 | 东华大学 | Method for preparing dexamethasone slow-release medicine carrier |
| KR101386096B1 (en) * | 2013-02-28 | 2014-04-21 | 강원대학교산학협력단 | Chitosan nanofiber for delivering anionic protein, a process for the preparation thereof, and transmucosal administrative agent comprising the chitosan nanofiber |
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