CN1039824C - Method of reproduction or fermentation for aerophile bacteria - Google Patents
Method of reproduction or fermentation for aerophile bacteria Download PDFInfo
- Publication number
- CN1039824C CN1039824C CN93104828A CN93104828A CN1039824C CN 1039824 C CN1039824 C CN 1039824C CN 93104828 A CN93104828 A CN 93104828A CN 93104828 A CN93104828 A CN 93104828A CN 1039824 C CN1039824 C CN 1039824C
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- CN
- China
- Prior art keywords
- liquid
- rotating bed
- oxygen
- gas
- inlet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/26—Conditioning fluids entering or exiting the reaction vessel
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- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a method for intensifying the propagation or the aerobic fermentation of aerobic bacterial strains by a rotating bed. In the method for intensifying the propagation or the aerobic fermentation of aerobic bacterial strains, mixed liquid comprising bacterium body circulating liquid and nutrient liquid is pumped into a rotating bed liquid inlet, gas comprising oxygen (air, oxygen-enriched air or pure oxygen) is led into a rotating bed gas inlet, after the mass transfer exchange of the liquid and the gas in a rotating bed filling material layer, the liquid is discharged from a rotating bed liquid outlet and is conveyed to a circulating tank, and the gas is emptied from a gas discharging port or is circularly used. The present invention considerably accelerates an oxygen dissolved process and considerably enhances the utilization rate of oxygen. The present invention shortens a production period and saves energy consumption.
Description
The present invention proposes a kind of breeding of good gas (oxygen consumption) bacterial classification in the rotating bed reinforcement liquid or processing method of aerobic fermentation utilized.The production process that is used for antibiotic, organic acid, single cell protein, zymin and biological pesticide etc.
Traditional usefulness liquid culture aerobic organism kind or aerobic fermentation technological process are that nutrient solution and bacterial classification are added in the jar, and air passes through a jar interior nutrient solution in the bubbling mode.The mass-transfer efficiency that this method gas phase oxygen dissolves in liquid is low, and most of oxygen is underutilized in the long and air of production cycle, blasts the energy overwhelming majority that air consumes and is wasted.
Utilize the effect of mechanical stirrer to strengthen the mixing effect of gas and nutrient solution or fermented liquid,, still have considerable oxygen not obtain utilizing though can obviously improve the mass-transfer efficiency of dissolved oxygen.Also have methods such as turbine type, self-priming and outer circulation air lift type.Its primary process does not all break away from air bubbling in liquid and passes through.Therefore, the efficient of dissolved oxygen and speed still do not obtain breakthrough raising.
Purpose of the present invention: change the way of contact of gas-liquid, utilize rotating bed to strengthen mass transfer between oxygen-containing gas and nutrient solution or the fermented liquid, propose a kind ofly to accelerate the dissolved oxygen process, improve the culture propagation of utilization ratio of oxygen or the processing method of fermentation, thus reach shorten the production cycle, improve throughput, the purpose of saving energy consumption.
In view of above-mentioned purpose, essential implementation of the present invention is:
1, adopts rotating bed equipment, with fresh nutritive medium with contain culture of strains liquid or fermented liquid (mycetome circulation fluid) together joins the rotating bed liquid-inlet, oxygen-containing gas (air, oxygen-rich air or pure oxygen) feeds the inlet mouth of rotating bed, mixing liquid is constantly torn up in packing layer, produce new surface in contact, and the mass transfer liquid of liquid phase is discharged from liquid outlet, send circulation tank back to.Gas is discharged emptying or is recycled from venting port.
2, nutritive medium mixes adding at rotating bed liquid-inlet place with the mycetome circulation fluid, or adds simultaneously from the circulation tank top or two.The mode that adds can be continuously, off and on or once.
Accompanying drawing 1 is an implementing procedure synoptic diagram of the present invention:
As shown in Figure 1, rotating bed is positioned at the circulation tank top, mixes with fresh nutrient solution from mycetome circulation fluid process pump 2 backs of circulation tank 1 bottom discharge mouth, together squeezes into the liquid-inlet 3 of rotating bed.Spray to ring packing 4 then, under action of centrifugal force, to around throw away, collect in rotating bed shell 5 bottoms, mixed solution is discharged from leakage fluid dram, sends circulation tank back to.Oxygen-containing gas enters from the gas feed of rotating bed, contacts with liquid in filler, and oxygen is passed to liquid, discharges from pneumatic outlet at last.
The present invention has changed traditional processing method, makes gas phase become external phase, and liquid phase becomes disperse phase, has not only increased mass transfer area but also reduced resistance to mass transfer, has shortened the production cycle, has improved throughput, and has saved energy consumption.With yeast production is example:
1, fermentation time shortened to 5-6 hour from 12 hours.
2, every production 1m
3The air consumption of fermented liquid is about original 1/3.
3, original production process gas bubbling will pass through several meters high liquid layer, and present method pressure drop has only tens millimeters to hundreds of millimeter water column, has reduced the power consumption of gas.
Claims (2)
1, a kind of breeding of aerobic organism kind in the rotating bed reinforcement liquid or method of aerobic fermentation utilized, it is characterized in that: the mixed solution of mycetome circulation fluid and nutritive medium is together added the rotating bed liquid-inlet, oxygen-containing gas feeds the inlet mouth of rotating bed, mixed solution contacts in ring packing with oxygen under centrifugal action, absorb mixed solution behind the oxygen and discharge from the rotating bed liquid exit and send circulation tank back to, gas is from venting port emptying or recycle.
2, method according to claim 1 is characterized in that: nutritive medium mixes with the mycetome circulation fluid at rotating bed liquid-inlet place or adds from the circulation tank top or add simultaneously two, and the mode of adding is successive or intermittence or disposable.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN93104828A CN1039824C (en) | 1993-05-08 | 1993-05-08 | Method of reproduction or fermentation for aerophile bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN93104828A CN1039824C (en) | 1993-05-08 | 1993-05-08 | Method of reproduction or fermentation for aerophile bacteria |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1076725A CN1076725A (en) | 1993-09-29 |
| CN1039824C true CN1039824C (en) | 1998-09-16 |
Family
ID=4985394
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN93104828A Expired - Fee Related CN1039824C (en) | 1993-05-08 | 1993-05-08 | Method of reproduction or fermentation for aerophile bacteria |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1039824C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110523178A (en) * | 2019-09-16 | 2019-12-03 | 温州捷朴环保科技有限公司 | A kind of smog pollution purification device based on hypergravity |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0048088A2 (en) * | 1980-09-11 | 1982-03-24 | Imperial Chemical Industries Plc | Centrifugal gas-liquid contact apparatus |
| SU1607848A1 (en) * | 1988-04-25 | 1990-11-23 | Предприятие П/Я Р-6956 | Rotary mass exchange apparatus |
| CN2109880U (en) * | 1991-11-27 | 1992-07-15 | 北京化工学院 | Transmit and reaction device for rotary bed superheavy field |
-
1993
- 1993-05-08 CN CN93104828A patent/CN1039824C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0048088A2 (en) * | 1980-09-11 | 1982-03-24 | Imperial Chemical Industries Plc | Centrifugal gas-liquid contact apparatus |
| SU1607848A1 (en) * | 1988-04-25 | 1990-11-23 | Предприятие П/Я Р-6956 | Rotary mass exchange apparatus |
| CN2109880U (en) * | 1991-11-27 | 1992-07-15 | 北京化工学院 | Transmit and reaction device for rotary bed superheavy field |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110523178A (en) * | 2019-09-16 | 2019-12-03 | 温州捷朴环保科技有限公司 | A kind of smog pollution purification device based on hypergravity |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1076725A (en) | 1993-09-29 |
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| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |