CN103977045A - Application of folium isatidis and extract thereof in preparing medicaments for preventing and treating alopecia and premature gray - Google Patents
Application of folium isatidis and extract thereof in preparing medicaments for preventing and treating alopecia and premature gray Download PDFInfo
- Publication number
- CN103977045A CN103977045A CN201410243745.0A CN201410243745A CN103977045A CN 103977045 A CN103977045 A CN 103977045A CN 201410243745 A CN201410243745 A CN 201410243745A CN 103977045 A CN103977045 A CN 103977045A
- Authority
- CN
- China
- Prior art keywords
- folium isatidis
- extract
- application
- extraction
- medicine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention develops a novel application of folium isatidis based on existing effects of the folium isatidis through massive experiment screening, and provides an optimal extraction process by means of screening of massive experiments. Experiment results indicate that the folium isatidis and extract thereof have good effects of promoting hair growth and preventing alopecia and premature gray, does not have remarkable adverse effect, and is safer and more reliable in administration.
Description
Technical field
The present invention relates to a kind of Chinese medicine, be specifically related to the application of Folium Isatidis in control alopecia, premature graying of hair, belong to Chinese medicine technical field.
Background technology
Alopecia, premature graying of hair are clinical common trichopathies, though do not belong to serious disease, spirit, psychology on people produce larger impact.In recent years, along with the development of modern society, social competition increasingly sharpens, and people strengthen gradually at the pressure of the each side such as work, study, life; And the quick raising of living standard, increases the food intake dose such as protein, fat in mass food and drink, and vitamin etc. are taken in and reduced.These all cause the sickness rate of alopecia increasing year by year, and age of onset is rejuvenation increasingly also.Alopecia can be divided into the polytypes such as alopecia areata, seborrheic alopecia, alopecia due to chemotherapy, senile alopecia, chronic telogen effluvim, post disease alopecia.
At present, the medicine of hair growth, premature graying of hair is a lot, has local external application medicine, also has Chinese medicine for oral administration and Western medicine, and the most of drug side effect of Western medicine is large, and human body is produced to injury, once stop using, the state of an illness recurs again, only cures the symptoms, not the disease; The patent of invention of the Chinese medicine of existing hair growth, premature graying of hair is all compound medicine combination, and composition is complicated, difficult quality control, and dose is large, has certain untoward reaction.
Summary of the invention
Goal of the invention: technical problem to be solved by this invention is, overcomes the deficiencies in the prior art, on the former powerful basis of Chinese medicine Folium Isatidis, filters out its new purposes in control alopecia, premature graying of hair by great many of experiments.
Technical scheme: in order to realize above object, the technical scheme that the present invention takes is:
The application of Folium Isatidis in preparation control alopecia, premature graying of hair medicine.
The application of Folium Isatidis extract in preparation control alopecia, premature graying of hair medicine, described Folium Isatidis extract prepares by the following method:
Get Folium Isatidis, with the mixed solvent of petroleum ether, dichloromethane, ethyl acetate one of them or they, adopt percolation to extract, merge extractive liquid,, lower than 60 DEG C of reclaim under reduced pressure, the concentrated and dry Folium Isatidis extract that obtains.
As another preferred version, the application of Folium Isatidis extract in preparation control alopecia, premature graying of hair medicine, described Folium Isatidis extract prepares by the following method:
Get Folium Isatidis and adopt the extraction of supercritical carbon dioxide method, obtain carbon dioxide abstraction liquid, obtaining Folium Isatidis extract lower than 60 DEG C of concentrating under reduced pressure with being dried.
As preferred version, the application of above-described Folium Isatidis extract in preparation control alopecia, premature graying of hair medicine, supercritical carbon dioxide method extraction conditions is: entrainer is ethanol, methanol, n-butyl alcohol, propylene glycol or isopropyl alcohol, entrainer volumetric usage (ml) is 0% to 50% of Folium Isatidis weight (g), extraction temperature is 20 DEG C to 50 DEG C, extraction pressure be 10Mpa to 40Mpa, extraction time is 1～4 hour.
As preferred version, the application of described described Folium Isatidis extract in preparation control alopecia, premature graying of hair medicine, it is characterized in that, supercritical carbon dioxide method extraction conditions is: entrainer is ethanol, ethanol volumetric usage (ml) is 25% of Folium Isatidis weight (g), 40 DEG C of extraction temperature, extraction pressure 35Mpa, extraction time 2 hours.
As preferred version, the application of described Folium Isatidis in preparation control alopecia, premature graying of hair medicine, described medicine is pill, powder, unguentum, sublimed preparation, medicated wine, electuary, oral solutions, capsule, tablet, gel or external preparation.
As preferred version, the application of described Folium Isatidis extract in preparation control alopecia, premature graying of hair medicine, it is characterized in that, described medicine is pill, powder, unguentum, sublimed preparation, medicated wine, electuary, oral solutions, capsule, tablet, gel or external preparation.
Beneficial effect: the present invention screens by great many of experiments, on the former powerful basis of Folium Isatidis, develop its new purposes, and filter out best extraction process by great many of experiments, optimize best process for super-critical extracting, experimental result shows, Folium Isatidis of the present invention and extract thereof have good promotion hair growth, anti-loss effect
Detailed description of the invention
According to following embodiment, the present invention may be better understood.But, those skilled in the art will readily understand, the described concrete material proportion of embodiment, process conditions and result thereof be only for the present invention is described, and should also can not limit the present invention described in detail in claims.
Embodiment 1
A kind of Folium Isatidis extract, it prepares by the following method: gets 2 kilograms of dry Folium Isatidiss, pulverizes, the dichloromethane percolation of 10 times of amounts leaf coarse powder for, percolate is lower than 60 DEG C of reclaim under reduced pressure, concentrated and be dried and obtain Folium Isatidis extract 93.0g.
Embodiment 2
A kind of Folium Isatidis extract, it prepares by supercritical extraction method: get 1.2 kilograms of dry Folium Isatidiss, pulverized 20 orders, 40 DEG C of extraction temperature, extracting pressure 35Mpa, carries out supercritical carbon dioxide extraction under the condition that the consumption of extraction time 2 hours and entrainer methanol is 300ml, be extracted thing, obtaining concentrated solution lower than 60 DEG C of reclaim under reduced pressure, dry, obtain Folium Isatidis extract 48g.
Embodiment 3
A kind of Folium Isatidis extract, it prepares by supercritical extraction method: get 1.2 kilograms of dry Folium Isatidiss, pulverized 20 orders, 50 DEG C of extraction temperature, extracting pressure 40Mpa, carries out supercritical carbon dioxide extraction under the condition that the consumption of extraction time 3 hours and entrainer ethanol is 300ml, be extracted thing, obtaining concentrated solution lower than 60 DEG C of reclaim under reduced pressure, dry, obtain Folium Isatidis extract 46g.
Embodiment 4
2% Carbomer and 10% glycerol are ground to even, add purified water appropriate, after fully swelling, add 20% propylene glycol, 1% Azone, 0.02% VE, get the each 20g of extract that embodiment 1 or 2 obtains respectively, stir, adjusting pH is 6.5, adds purified water to 1000g, stirs and obtains Anti-hair loss gel preparation.
Embodiment 5
Get respectively the extract that embodiment 1 or 2 obtains, add 5000g water, 750g cocos nucifera oil phthalein amine CAB by every 20g, 350g coconut oleoyl amine DEA, mix homogeneously, obtains external Anti-hair loss hair washing preparation.
Embodiment 6 Folium Isatidis extract hair regrowth experimentatioies
1, experiment grouping:
Rat is divided 5 groups at random, every group 12, Folium Isatidis extract that the embodiment of the present invention 1 prepares is little, in, heavy dose, be respectively 0.75g/kg, 1.5g/kg and 3.0g/kg, administration volume is 10ml/kg, matched group Alopecia Pill dosage is 3.0g/kg, gastric infusion, and blank group is to equal-volume normal saline.
2, experimental technique:
Each group rat is in advance prior to back same area cropping, and cropping area is 3 × 3cm, starts administration the same day in cropping, and be administered once every day, continuous 7 days, within the 8th day, cuts newborn hair, electronic balance precise weighing at cropping position last time.
3, experimental result, experimental result shows, and in Folium Isatidis extract provided by the invention, heavy dose can obviously promote cropping rats hair growth, newborn gross weight amount is apparently higher than matched group, and specific experiment result is as shown in table 1.
Table 1 Folium Isatidis extract on rat cropping after the impact (Mean ± SD) of hair growth
With relatively * p<0.05 of matched group, * * p<0.01
Embodiment 7
1, experiment grouping:
Separately get 40 of Cavia porcelluss, be divided at random 5 groups, Folium Isatidis extract that the embodiment of the present invention 2 prepares is little, in, heavy dose of 0.5g/kg, 1.0g/kg and 2.0g/kg, administration volume is 10ml/kg, matched group Alopecia Pill dosage is 2.0g/kg, gastric infusion, blank group is to equal-volume normal saline.
2, experimental technique:
Each group Cavia porcellus is in advance prior to back same area cropping, and cropping area is 3 × 3cm, starts administration the same day in cropping, and be administered once every day, continuous 7 days, within the 8th day, cuts newborn hair, electronic balance precise weighing at cropping position last time.
3, experimental result
Experimental result shows, the each dosage of Folium Isatidis extract provided by the invention all has the effect of certain promotion cropping Cavia porcellus hair growth, and heavy dose of effect obviously, presents good dosage medicine efficacy relation, newborn gross weight amount is apparently higher than matched group, and specific experiment the results are shown in Table shown in 2.
Table 2 Folium Isatidis extract on Cavia porcellus cropping after the impact (Mean ± SD) of hair growth
| Group | Mus number (only) | Dosage (g/kg) | Newborn gross weight amount (mg) |
| Matched group | 8 | - | 89.1±23.5 |
| Folium Isatidis extract group | 8 | 0.5 | 102.3±20.7 |
| Folium Isatidis extract group | 8 | 1.0 | 118.9±27.4 |
| Folium Isatidis extract group | 8 | 2.0 | 128.2±32.4* |
| Alopecia Pill group | 8 | 2.0 | 118.5±17.8* |
With relatively * p<0.05 of matched group
Embodiment 8
Choose the routine early whitening of hair patient of outpatient service 64, the age 16 is to 35 years old, uses Folium Isatidis external Anti-hair loss hair washing preparation that the embodiment of the present invention 5 prepares after two months, and poliosis obviously reduces, and effective percentage 86% has demonstrated effect of good control early whitening of hair.
Show by above experimental result, Folium Isatidis extract provided by the invention has good control alopecia and white effect early, and in experimentation there are no untoward reaction, medication is safer.
Above embodiment is only explanation technical conceive of the present invention and feature; its object is to allow person skilled in the art understand content of the present invention and implemented; can not limit the scope of the invention with this; all equivalences that spirit is done according to the present invention change or modify, and all should be encompassed in protection scope of the present invention.
Claims (7)
1. the application of Folium Isatidis in preparation control alopecia, premature graying of hair medicine.
2. the application of Folium Isatidis extract in preparation control alopecia, premature graying of hair medicine, is characterized in that, Folium Isatidis extract prepares by the following method:
Get Folium Isatidis, with the mixed solvent of petroleum ether, dichloromethane, ethyl acetate one of them or they, adopt percolation to extract, merge extractive liquid,, lower than 60 DEG C of reclaim under reduced pressure, the concentrated and dry Folium Isatidis extract that obtains.
3. the application of Folium Isatidis extract in preparation control alopecia, premature graying of hair medicine, is characterized in that, Folium Isatidis extract prepares by the following method:
Get Folium Isatidis and adopt the extraction of supercritical carbon dioxide method, obtain carbon dioxide abstraction liquid, obtaining Folium Isatidis extract lower than 60 DEG C of concentrating under reduced pressure with being dried.
4. the application of Folium Isatidis extract according to claim 3 in preparation control alopecia, premature graying of hair medicine, it is characterized in that, supercritical carbon dioxide method extraction conditions is: entrainer is ethanol, methanol, n-butyl alcohol, propylene glycol or isopropyl alcohol, entrainer volumetric usage is 0% to 50% of Folium Isatidis weight, extraction temperature is 20 DEG C to 50 DEG C, extraction pressure be 10Mpa to 40Mpa, extraction time is 1 ~ 4 hour.
5. the application of Folium Isatidis extract according to claim 3 in preparation control alopecia, premature graying of hair medicine, it is characterized in that, supercritical carbon dioxide method extraction conditions is: entrainer is ethanol, ethanol volumetric usage is 25% of Folium Isatidis weight, 40 DEG C of extraction temperature, extraction pressure 35 Mpa, extraction time 2 hours.
6. the application of Folium Isatidis according to claim 1 in preparation control alopecia, premature graying of hair medicine, it is characterized in that, described medicine is pill, powder, unguentum, sublimed preparation, medicated wine, electuary, oral solutions, capsule, tablet, gel or external preparation.
7. the application of Folium Isatidis extract according to claim 2 in preparation control alopecia, premature graying of hair medicine, it is characterized in that, described medicine is pill, powder, unguentum, sublimed preparation, medicated wine, electuary, oral solutions, capsule, tablet, gel or external preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410243745.0A CN103977045A (en) | 2014-06-03 | 2014-06-03 | Application of folium isatidis and extract thereof in preparing medicaments for preventing and treating alopecia and premature gray |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410243745.0A CN103977045A (en) | 2014-06-03 | 2014-06-03 | Application of folium isatidis and extract thereof in preparing medicaments for preventing and treating alopecia and premature gray |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103977045A true CN103977045A (en) | 2014-08-13 |
Family
ID=51269372
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410243745.0A Pending CN103977045A (en) | 2014-06-03 | 2014-06-03 | Application of folium isatidis and extract thereof in preparing medicaments for preventing and treating alopecia and premature gray |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103977045A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105725621A (en) * | 2016-03-08 | 2016-07-06 | 宁波高新区苗姑养生科技有限公司 | Healthcare pillow for preventing and treating alopecia |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0517365A (en) * | 1991-07-03 | 1993-01-26 | Nonogawa Shoji Kk | Testosterone 5alpha-reductase inhibitor |
| CN1251757A (en) * | 1998-10-21 | 2000-05-03 | 赵舜英 | Oral liquid for beautifying face and growing hair and its preparing process |
| CN101658518A (en) * | 2009-08-19 | 2010-03-03 | 刘庆华 | Application of indirubin for preparing hair culture |
-
2014
- 2014-06-03 CN CN201410243745.0A patent/CN103977045A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0517365A (en) * | 1991-07-03 | 1993-01-26 | Nonogawa Shoji Kk | Testosterone 5alpha-reductase inhibitor |
| CN1251757A (en) * | 1998-10-21 | 2000-05-03 | 赵舜英 | Oral liquid for beautifying face and growing hair and its preparing process |
| CN101658518A (en) * | 2009-08-19 | 2010-03-03 | 刘庆华 | Application of indirubin for preparing hair culture |
Non-Patent Citations (3)
| Title |
|---|
| 丁改法等: "中草药提取技术的概述", 《中国兽药杂志》 * |
| 国家中医药管理局《中华本草》编委会编著: "《中华本草 维吾尔药卷》", 31 December 2005 * |
| 李慧等: "大青叶中靛玉红的超临界萃取工艺及含量测定", 《第四军医大学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105725621A (en) * | 2016-03-08 | 2016-07-06 | 宁波高新区苗姑养生科技有限公司 | Healthcare pillow for preventing and treating alopecia |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103768152B (en) | Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract and its production and use | |
| CN105395919A (en) | Composition containing black fungus extract and having effect of reducing blood fat and preparation method of composition | |
| CN103181560B (en) | Health food having effects of life cultivation and health preservation in winter and preparation method thereof | |
| CN103977045A (en) | Application of folium isatidis and extract thereof in preparing medicaments for preventing and treating alopecia and premature gray | |
| CN108126052A (en) | A kind of preparation process for nourishing refreshing particle | |
| CN102657730A (en) | Rhodiola rosea buccal preparations for resisting altitude reaction | |
| CN102579530A (en) | Preparation method of aralia taibaiensis total saponin having diabetes mellitus resisting effect and medicament | |
| CN104940263A (en) | Composition with functions of alleviating hangover and protecting liver and preparation method of composition | |
| CN104804056A (en) | Sarcopyramis nepalensis extract and application thereof | |
| CN105998104B (en) | A kind of purposes of fruits of elm extract in terms of nerve protection medicine is treated in preparation | |
| CN104546987A (en) | Application of gynostemma pentaphyllum total saponins to prevention and cure of hyperuricemia | |
| CN107137416A (en) | A kind of pharmaceutical composition for preventing and treating non-small cell lung cancer | |
| CN100471515C (en) | Natural anticancer preparation and process and use thereof | |
| CN105663675A (en) | Traditional Chinese medicine composition with anti-fatigue function as well as preparation method and application of traditional Chinese medicine composition | |
| CN105267580A (en) | Application of dianella ensifolia or extracts thereof | |
| CN1449799A (en) | Traditional Chinese medicine injection for treating tumor and preparation process thereof | |
| CN102488212A (en) | Health food used for improving sleep, and preparation method thereof | |
| CN104887796A (en) | Anti-altitude-stress rhodiola rosea oral liquid and preparation method thereof | |
| CN109337783A (en) | A kind of tranquilizing the mind heart-nourishing liquor and preparation method thereof | |
| CN103230456A (en) | Traditional Chinese medicine extract and preparation method thereof | |
| CN116870066B (en) | Method for efficiently preparing qu bitter orange total flavonoids and application thereof | |
| CN102526387A (en) | Medicinal composition for treating early-stage diabetic foot and preparation method thereof | |
| CN109045239B (en) | Traditional Chinese medicine extract, preparation method thereof and application thereof in preparation of anti-cancer drugs | |
| CN102631486B (en) | Health care composition | |
| CN105616706A (en) | Shenling (panax quinquefolius and ganoderma lucidum) granules for strengthening body resistance and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20140813 |