CN103966128B - One strain Thiobacillus Thioparaus and the application in biological desulphurization thereof - Google Patents
One strain Thiobacillus Thioparaus and the application in biological desulphurization thereof Download PDFInfo
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- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
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- 229940041514 candida albicans extract Drugs 0.000 description 2
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- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- 241000605222 Acidithiobacillus ferrooxidans Species 0.000 description 1
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- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- XUYPXLNMDZIRQH-LURJTMIESA-N N-acetyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC(C)=O XUYPXLNMDZIRQH-LURJTMIESA-N 0.000 description 1
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- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
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- NIFIFKQPDTWWGU-UHFFFAOYSA-N pyrite Chemical compound [Fe+2].[S-][S-] NIFIFKQPDTWWGU-UHFFFAOYSA-N 0.000 description 1
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Abstract
One strain Thiobacillus Thioparaus and the application in biological desulphurization thereof, belong to biological and environmental protection technical field.The invention discloses a strain addicted to salt sulfur-oxidizing bacteria, does is Classification And Nomenclature Thiobacillus Thioparaus? (<i>Thiobacillus</i><i>? neapolitanus</i>)? CYJN-1, being preserved in China typical culture collection center, does is deposit number: CCTCC? NO:M? 2014129.This bacterium, has stronger oxidation capacity to the sulfide of reduction-state, elemental sulfur and thiosulphate under certain condition.Thiobacillus Thioparaus of the present invention can tolerate the physical environment compared with high salinity, is expected to be applied to that salinity is higher, the natural biology desulfurization system of mild condition.
Description
Technical field
The present invention relates to the Thiobacillus Thioparaus of a strain addicted to salt, can be applicable to biogas, selexol process and stack gas SO
2the biological removal of the sulfide that absorption liquid produces through bio anaerobic reduction.Belong to biological and environmental protection technical field.
Background technology
The release of sulfide is the subject matter of anaerobic treatment association when being rich in sulfate wastewater.Namely sulphate reducing bacteria (SRB) is with SO
4 2-as electron acceptor(EA), participate in organic catabolism, and by most SO
4 2-with S
2-form is discharged into cells in vitro.Due to the toxicity of sulfide, corrodibility, stench and high Problem of Oxygen Consumption, must adopt an effective measure controls it and discharges in environment.Traditional sulfur method technical sophistication, cost is high, and byproduct is difficult to process.Biological desulfurizing technology service temperature and pressure low, invest less, no waste mine discharge, have broad prospects.
The research of using microbe desulfurization starts along with utilizing the research of microorganism ore dressing.Nineteen forty-seven, Colmer and Hinkle finds and confirms that chemosynthetic bacteria can accelerating oxidation dissolve the pyrite existed in coal, and this is considered to the beginning of Biohydrometallurgy research.The fifties in last century, first such bacterium was used for desulfurization research by Leathan etc. to the sixties.Silverman have studied the pyritous mechanism of Thiobacillus Ferrooxidans'Oxidation and physiological property, confirms that it can accelerate pyritous oxidation.At the end of the seventies, Kargi has found that a kind of thermophilic thiobacillus is better for sweetening effectiveness, can remove the pyritic sulphur of 100% and the organosulfur of 40%.At the beginning of the nineties, build up at Italian Torres port the pilot plant that microorganism removes pyritic sulphur, put into operation, for the data that provide the foundation are amplified in industry.The nineties, China Xu Yi, Zhong Huifang etc. remove pyritic sulphur with thiobacillus ferrooxidant and also achieve good effect.
In desulfurization microorganism, Thiobacillus is modal colorless sulfur bacteria, this bacterial classification shape is thin rod-short, to hold raw flagellar movement, Gram-negative, strict autotrophy, obtaining energy by redox sulfide, can be elemental sulfur (getting rid of external) or vitriol by sulfide-oxidation according to culture condition.But colorless sulfur bacteria desulfurization at present is still in the preliminary research stage, and sulfur recovery rate is lower, and industrialization degree is not high, traces it to its cause, and does not mainly have desirable high-efficiency desulfurization bacterium.In addition, the control of colorless sulfur bacteria sulphur pathways metabolism also has influence on the industrial applications of function yeast.
Therefore, Screening and Identification high-efficiency desulfurization bacterium is also applied to the removal of sulfide, improves desulfuration efficiency and elemental sulfur productive rate further, reduces production cost and just become extremely important.
Summary of the invention
Main purpose of the present invention is exactly for above Problems existing and deficiency, provides the application of desulfurization bacterium for biological desulphurization of a strain efficient removal sulfide.
Technical scheme of the present invention: a strain sulfur-oxidizing bacteria, Thiobacillus Thioparaus (
thiobacillusneapolitanus) CYJN-1, oneself is preserved in China typical culture collection center on April 13rd, 2014, and deposit number is: CCTCCNO:M2014129.
The application of described bacterial strain CCTCCNO:M2014129, this bacterial strain cannot utilize various organism, but sodium sulphite, elemental sulfur and thiosulphate can be utilized, reduced sulfur compound efficient oxidation is generated elemental sulfur and vitriol, batch culture shows, this bacterial strain achieves the thiosulphate conversion of 99% in 24h, and optimal salinity is 2%-5%, optimal pH is 7.0, optimum temperuture is 30 DEG C.This bacterial strain is used for biological desulphurization.
Described Thiobacillus Thioparaus (
thiobacillusneapolitanus) screening method of CYJN-1, adopt thiosulfuric acid salt culture medium enrichment culture 4 days, repeats 3-4 time, employing Na
2s
2o
35H
2o substratum carries out plate isolation, and after 3 days, bacterium colony generates, and picking list bacterium colony enters thiosulphate culture medium culturing, repeats to obtain pure bacterium 4 times.
The invention provides a strain Thiobacillus Thioparaus, from power-plant cooling water pond, Waigaoqiao of Shaihai, screening also obtains through artificially breeding, called after CYJN-1, is rod-short, Gram-negative and comparatively thick-walled pod film.This bacterium the most suitable growth pH is 7.0, and optimum growth temperature is 30 DEG C.The neutral pH of generation elemental sulfur and lesser temps are conducive to the design and the convenient operation that simplify reactor.
The invention provides the Physiology and biochemistry authentication method of described bacterium, be characterized in, the optimum growth temperature scope of this bacterium is 25-35 DEG C, and optimal pH scope is 6-8, and optimal salinity scope is at 2%-5%.At the temperature and pH condition of optimum, can not organism be utilized and the generation of organism to thalli growth and elemental sulfur has restraining effect in various degree, can the element sulphur of efficiency utilization reduction-state.Under transmission electron microscope, cell is shaft-like, and is wrapped up by the pod membrane that one deck is thicker.Through 16SrRNA qualification, CYJN-1 has exceeded 99% with the sibship addicted to salt Thiobacillus.
Beneficial effect of the present invention: the invention provides a kind of high-efficiency desulfurization bacterium being energy substrate with thiosulphate (the aerial major oxidation product of sulfide) and carry out sulfide minimizing technology in batches.Be characterized in, adopt the pure bacterium of CYJN-1 and thiosulfate solution to carry out sulfide removal, under optimal pH and temperature, achieve the thiosulphate transformation efficiency of 99% in 24h, have higher desulfuration efficiency.
Compared with the bacterial classification that bacterial strain provided by the invention is common with other biological desulphurization, described bacterium can tolerate higher salinity, mild condition, removes the sulfide of reduction-state efficiently, better can adapt to the natural system of biological desulphurization.
Biological material specimens preservation: Thiobacillus Thioparaus (
thiobacillusneapolitanus) CYJN-1; Be deposited in China typical culture collection center, be called for short CCTCC, address: Wuhan, China Wuhan University, preservation date on April 13rd, 2014, inclined number of preservation is: CCTCCNO:M2014129.
Accompanying drawing explanation
Fig. 1 adopts the cellular form of Thiobacillus Thioparaus of the present invention under transmission electron microscope.
Fig. 2 is the changing conditions of various factors in the Thiobacillus Thioparaus CYJN-1 batch process that adopts of the present invention.
Embodiment
Embodiment 1 bacterial strain screening
Get in the present embodiment in power-plant cooling water pond, Shanghai and get appropriate mud, pour in sterilized water, concussion makes soil sample fully break up, then accessed in enrichment medium, inoculum size by 5% adds in the 250mL triangular flask containing 100mL thiosulfuric acid salt culture medium, pH=7,30 DEG C, 170r/min, carry out enrichment culture 4 days, repeat 3-4 time, Sulfothiorine substratum is adopted to carry out plate isolation, after 3 days, bacterium colony generates, and picking list bacterium colony access Sulfothiorine culture medium culturing, repeats plate isolation and obtain pure bacterium 4 times.
Embodiment 2 identification of strains
Adopt bacterium full-length genome Rapid extraction test kit, extract the full-length genome of pure growth, and carry out PCR by 16SrDNA universal primer, identify that bacterial strain is Thiobacillus Thioparaus, called after CYJN-1.
Embodiment 3 bacterial strain Physiology and biochemistry and Resistence research
Bacterial strain CYJN-1 to be inoculated in the shaking table of differing temps in (10,20,30,40,50 DEG C) liquid nutrient medium by identical inoculum size, to determine that optimum growth temperature is 30 DEG C; Take initial pH3.0,4.0,5.0,6.0,7.0,8.0 and 9.0 to cultivate under optimum temperuture, determine that the most suitable growth pH is 7.0.By the one in following substrate component, determine the best energy substrate (g/L) of bacterial classification CYJN-1: substrate is respectively: yeast extract 1.0; Peptone 1.0; Glucose 1.0; Fructose 1.0; Sucrose 1.0; Lactose 1.0; Semi-lactosi 1.0; Maltose 1.0; Glycine 1.0; Methionin 1.0; Methionine(Met) 1.0; Na
2s
2o
35H
2o10.0; Na
2s1.0; S
01.0; Yeast extract 1.0 and Na
2s
2o
35H
2o10.0; Peptone 1.0 and Na
2s
2o
35H
2o10.0; Glucose 1.0 and Na
2s
2o
35H
2o10.0.Determine CYJN-1 and cannot utilize various organism, but can utilize elemental sulfur, sodium sulphite and Sulfothiorine, its best energy substrate is Sulfothiorine.
Embodiment 4 biological desulphurization
Determining, under the initial pH of above-mentioned optimum growh, temperature and concentration of substrate, to study the degradation property of this bacterial strain to thiosulfate solution.The substratum getting the configuration of 100mL Sulfothiorine is placed in 250mL Erlenmeyer flask, and inoculation 5mL bacterium liquid, cultivate under 170r/min, its growth curve and chemical index are as shown in Figure 2.Can find out, just cultivated 12h inner cell and be in the lag phase conformed, the poor growth of bacterial strain, growth curve is horizontal line shape; And in 30h subsequently, cell enters Exponential growth stage, grow vigorous, the speed of growth is exceedingly fast, and cell quantity is that multiple increases, and growth curve rises in rain; Afterwards because substratum nutritive substance is limited, and cell quantity is too much, vies each other, so the cell count of death and newborn cell count maintain an equal level, enters the stage of stable development.In culturing process, the decline of thiosulfate concentration along with the rising of sulfate concentration, this show bacterial strain CYJN-1 take elemental sulfur as mesostate by thiosulphate-sulphur of divalent is oxidized to vitriol.Batch culture result shows: bacterial strain CYJN-1, under optimum growing condition, can reach 99% to the decreasing ratio of Sulfothiorine within 24 hours.
In sum, of the present invention higher addicted to salt thiobacillus sweetening power, be expected to be applied to salinity higher, in the natural desulfurization system of mild condition.
In this description, the present invention is described with reference to its specific embodiment.But, still can make various amendment and conversion obviously and not deviate from the spirit and scope of the present invention.Therefore, specification sheets and accompanying drawing are regarded in an illustrative, rather than a restrictive.
Claims (1)
1. a strain Thiobacillus Thioparaus (
thiobacillusneapolitanus) application of CCTCCNO:M2014129, it is characterized in that for biological desulphurization, this bacterial strain cannot utilize various organism, but sodium sulphite, elemental sulfur and thiosulphate can be utilized, reduced sulfur compound efficient oxidation is generated elemental sulfur and vitriol, batch culture shows, and this bacterial strain achieves the thiosulphate conversion of 99% in 24h, and optimal salinity is 2%-5%, optimal pH is 7.0, optimum temperuture is 30 DEG C.
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| CN105441345B (en) * | 2015-12-21 | 2019-02-22 | 宁波大学 | One plant of Candida tropicalis and its application in biological desulphurization |
| CN106591175B (en) * | 2016-11-10 | 2019-08-23 | 中国科学院成都生物研究所 | One plant of desulfurization Xu not prosperous Salmonella and its application |
| CN106434486B (en) * | 2016-11-10 | 2019-06-14 | 中国科学院成都生物研究所 | The red bacterium of one plant of desulfurization and its application |
| CN116426440B (en) * | 2023-05-26 | 2023-08-18 | 海南热带海洋学院 | Novel marine sulfur oxidizing bacteria strain and application thereof |
| CN117070424B (en) * | 2023-10-10 | 2023-12-22 | 华南理工大学 | Energy-converting autotrophic thiobacillus haloxydans strain and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102807960A (en) * | 2011-06-03 | 2012-12-05 | 中国科学院过程工程研究所 | Alkali-resistant thhiobacillus thioparus and application thereof |
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| CN102807960A (en) * | 2011-06-03 | 2012-12-05 | 中国科学院过程工程研究所 | Alkali-resistant thhiobacillus thioparus and application thereof |
Non-Patent Citations (2)
| Title |
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| 一株脱硫自养菌的鉴定和脱硫特性研究;吴晓雨;《微生物学通报》;20091231;620-626 * |
| 高效脱硫菌的筛选及脱硫性能研究;张娟;《河北建筑科技学院学报》;20060331;5-16 * |
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