CN103966128A - Thiobacillus neapolitanus and application of thiobacillus neapolitanus in biological desulfurization - Google Patents
Thiobacillus neapolitanus and application of thiobacillus neapolitanus in biological desulfurization Download PDFInfo
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- CN103966128A CN103966128A CN201410179292.XA CN201410179292A CN103966128A CN 103966128 A CN103966128 A CN 103966128A CN 201410179292 A CN201410179292 A CN 201410179292A CN 103966128 A CN103966128 A CN 103966128A
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- 241000605178 Halothiobacillus neapolitanus Species 0.000 title claims abstract description 12
- 238000006477 desulfuration reaction Methods 0.000 title abstract description 14
- 230000023556 desulfurization Effects 0.000 title abstract description 12
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims abstract description 21
- 230000003647 oxidation Effects 0.000 claims abstract description 7
- 238000007254 oxidation reaction Methods 0.000 claims abstract description 7
- 230000001580 bacterial effect Effects 0.000 claims description 18
- 241000605118 Thiobacillus Species 0.000 claims description 17
- DHCDFWKWKRSZHF-UHFFFAOYSA-L thiosulfate(2-) Chemical compound [O-]S([S-])(=O)=O DHCDFWKWKRSZHF-UHFFFAOYSA-L 0.000 claims description 7
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 claims description 6
- 235000010265 sodium sulphite Nutrition 0.000 claims description 3
- 150000003464 sulfur compounds Chemical class 0.000 claims description 2
- 241000894006 Bacteria Species 0.000 abstract description 25
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 abstract description 12
- DHCDFWKWKRSZHF-UHFFFAOYSA-N sulfurothioic S-acid Chemical compound OS(O)(=O)=S DHCDFWKWKRSZHF-UHFFFAOYSA-N 0.000 abstract description 6
- 238000004321 preservation Methods 0.000 abstract description 4
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 230000009467 reduction Effects 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 8
- 239000005864 Sulphur Substances 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- 239000011734 sodium Substances 0.000 description 6
- PODWXQQNRWNDGD-UHFFFAOYSA-L sodium thiosulfate pentahydrate Chemical compound O.O.O.O.O.[Na+].[Na+].[O-]S([S-])(=O)=O PODWXQQNRWNDGD-UHFFFAOYSA-L 0.000 description 6
- 239000000758 substrate Substances 0.000 description 6
- 229910052717 sulfur Inorganic materials 0.000 description 5
- 239000011593 sulfur Substances 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 108020004465 16S ribosomal RNA Proteins 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 239000000498 cooling water Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 229930182817 methionine Natural products 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- 241000605222 Acidithiobacillus ferrooxidans Species 0.000 description 1
- XTHFKEDIFFGKHM-UHFFFAOYSA-N Dimethoxyethane Chemical compound COCCOC XTHFKEDIFFGKHM-UHFFFAOYSA-N 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- XUYPXLNMDZIRQH-LURJTMIESA-N N-acetyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC(C)=O XUYPXLNMDZIRQH-LURJTMIESA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
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- 239000006227 byproduct Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000003245 coal Substances 0.000 description 1
- 230000009514 concussion Effects 0.000 description 1
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- 230000003247 decreasing effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000003009 desulfurizing effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethanethiol Chemical compound CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000013439 flagellum movement Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
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- 239000012528 membrane Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- NIFIFKQPDTWWGU-UHFFFAOYSA-N pyrite Chemical compound [Fe+2].[S-][S-] NIFIFKQPDTWWGU-UHFFFAOYSA-N 0.000 description 1
- 229910052683 pyrite Inorganic materials 0.000 description 1
- 239000011028 pyrite Substances 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 229910021653 sulphate ion Inorganic materials 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses thiobacillus neapolitanus and application of thiobacillus neapolitanus in biological desulfurization, and belongs to the technical field of biology and environmental protection. The invention discloses a strain of halophilic sulfur-oxidizing bacterium, and the classification and name are thiobacillus neapolitanus CYJN-1; the thiobacillus neapolitanus is preserved in China Center for Type Culture Collection with the preservation number of CCTCCNO. M2014129; the thiobacillus neapolitanus has relatively high capability of oxidation on sulfide in reduction state, elemental sulfur and thiosulfate; the thiobacillus neapolitanus provided by the invention can endure a natural environment with relative high salinity, and can be expected to be applied to natural biological desulfurization system with relatively high salinity and mild conditions.
Description
Technical field
The present invention relates to a strain and have a liking for the Thiobacillus Thioparaus of salt, can be applicable to biogas, selexol process and stack gas SO
2the biological removal of the sulfide that absorption liquid produces through bio anaerobic reduction.Belong to biology and environmental protection technical field.
Background technology
The release of sulfide is the subject matter of anaerobic treatment association while being rich in sulfate wastewater.Be that sulphate reducing bacteria (SRB) is with SO
4 2-as electron acceptor(EA), participate in organic catabolism, and by most SO
4 2-with S
2-form is discharged into cells in vitro.Due to toxicity, corrodibility, stench and the high Problem of Oxygen Consumption of sulfide, must adopt an effective measure control its in environment, discharge.Traditional sulfur method technical sophistication, cost is high, and byproduct is difficult to process.Biological desulfurizing technology service temperature and pressure are low, invest less, without waste material discharge, have broad prospects.
The research of using microbe desulfurization is accompanied by and utilizes the research of microorganism ore dressing to start.Nineteen forty-seven, Colmer and Hinkle find and confirm that chemosynthetic bacteria can accelerating oxidation and dissolve the pyrite existing in coal, and this is considered to the beginning of Biohydrometallurgy research.The fifties in last century, first Leathan etc. were used for desulfurization research by such bacterium to the sixties.Silverman has studied the pyritous mechanism of Thiobacillus Ferrooxidans'Oxidation and physiological property, has confirmed that it can accelerate pyritous oxidation.At the end of the seventies, Kargi has found that a kind of thermophilic thiobacillus is better for sweetening effectiveness, can remove 100% pyritic sulphur and 40% organosulfur.The beginning of the nineties, build up microorganism at Italian Torres port and remove the pilot plant of pyritic sulphur, put into operation, for the data that provide the foundation are amplified in industry.The nineties, China Xu Yi, Zhong Huifang etc. remove pyritic sulphur with thiobacillus ferrooxidant and have also obtained good effect.
In desulfurization microorganism, Thiobacillus is modal colorless sulfur bacteria, this bacterial classification is shaped as thin rod-short, to hold raw flagellar movement, Gram-negative, strict autotrophy, obtain energy by redox sulfide, can sulfide be oxidized to elemental sulfur (getting rid of external) or vitriol according to culture condition.But colorless sulfur bacteria desulfurization is at present still in the preliminary research stage, and sulfur recovery rate is lower, and industrialization degree is not high, traces it to its cause, and being does not mainly have desirable high-efficiency desulfurization bacterium.In addition, the control of colorless sulfur bacteria sulphur pathways metabolism also has influence on the industrial applications of function yeast.
Therefore, Screening and Identification high-efficiency desulfurization bacterium is also applied to the removal of sulfide, further improves desulfuration efficiency and elemental sulfur productive rate, reducing production costs just becomes extremely important.
Summary of the invention
Main purpose of the present invention is exactly the problems and shortcomings for above existence, provides a plant height effect to remove the desulfurization bacterium of sulfide for the application of biological desulphurization.
Technical scheme of the present invention: a strain sulfur-oxidizing bacteria, Thiobacillus Thioparaus (
thiobacillus neapolitanus) CYJN-1, oneself is preserved in Chinese Typical Representative culture collection center on April 13rd, 2014, and deposit number is: CCTCC NO:M 2014129.
The application of described bacterial strain CCTCC NO:M 2014129, this bacterial strain cannot utilize various organism, but can utilize sodium sulphite, elemental sulfur and thiosulphate, reduced sulfur compound efficient oxidation is generated to elemental sulfur and vitriol, batch culture shows, this bacterial strain has been realized 99% thiosulphate and has been transformed in 24h, and optimal salinity is that 2%-5%, optimal pH are 7.0, optimum temperuture is 30 DEG C.This bacterial strain is for biological desulphurization.
Described Thiobacillus Thioparaus (
thiobacillus neapolitanus) screening method of CYJN-1, adopt thiosulfuric acid salt culture medium enrichment culture 4 days, repeats employing Na 3-4 time
2s
2o
35H
2o substratum carries out plate isolation, and after 3 days, bacterium colony generates, and picking list bacterium colony enters thiosulphate culture medium culturing, repeats to obtain pure bacterium 4 times.
The invention provides a strain Thiobacillus Thioparaus, screen and pass through artificially breeding and obtain from power-plant cooling water pond, Waigaoqiao of Shaihai, called after CYJN-1, for rod-short, Gram-negative and compared with thick-walled pod film.This bacterium the most suitable growth pH is 7.0, and optimum growth temperature is 30 DEG C.The neutral pH of generation elemental sulfur and lesser temps are conducive to simplify design and the convenient operation of reactor.
The Physiology and biochemistry authentication method that the invention provides described bacterium, is characterized in, the optimum growth temperature scope of this bacterium is 25-35 DEG C, and optimal pH scope is 6-8, and optimal salinity scope is at 2%-5%.Under optimum temperature and pH condition, can not utilize organism and organism to have restraining effect in various degree to the generation of thalli growth and elemental sulfur, can efficiently utilize the element sulphur of reduction-state.Under transmission electron microscope, cell is shaft-like, and is wrapped up by the thicker pod membrane of one deck.Through 16S rRNA qualification, CYJN-1 has exceeded 99% with the sibship of having a liking for salt Thiobacillus.
Beneficial effect of the present invention: the invention provides one remove method taking thiosulphate (the aerial main oxidation products of sulfide) as the high-efficiency desulfurization bacterium of energy substrate carries out sulfide in batches.Be characterized in, adopt the pure bacterium of CYJN-1 and thiosulfate solution to carry out sulfide removal, at optimal pH and temperature, in 24h, realized 99% thiosulphate transformation efficiency, have higher desulfuration efficiency.
Compared with the bacterial strain provided by the invention bacterial classification common with other biological desulphurization, described bacterium can tolerate higher salinity, and mild condition removes the sulfide of reduction-state efficiently, can better adapt to the natural system of biological desulphurization.
Biological material specimens preservation: Thiobacillus Thioparaus (
thiobacillus neapolitanus) CYJN-1; Be deposited in Chinese Typical Representative culture collection center, be called for short CCTCC, address: Wuhan, China Wuhan University, preservation date on April 13rd, 2014, preservation has been for inclined to one side number: CCTCC NO:M 2014129.
Brief description of the drawings
Fig. 1 adopts the cellular form of Thiobacillus Thioparaus of the present invention under transmission electron microscope.
Fig. 2 is the changing conditions of various factors in the Thiobacillus Thioparaus CYJN-1 batch culture process that adopts of the present invention.
Embodiment
Embodiment 1 bacterial strain screening
Get in the present embodiment in power-plant cooling water pond, Shanghai and get appropriate mud, pour in sterilized water, concussion is fully broken up soil sample, then accessed in enrichment medium, add by 5% inoculum size in the 250mL triangular flask that contains 100mL thiosulfuric acid salt culture medium, pH=7,30 DEG C, 170r/min, carry out enrichment culture 4 days, repeat 3-4 time, adopt Sulfothiorine substratum to carry out plate isolation, after 3 days, bacterium colony generates, and picking list bacterium colony access Sulfothiorine culture medium culturing, repeats plate isolation and obtain pure bacterium 4 times.
Embodiment 2 identification of strains
Adopt the quick extraction agent box of the full genome of bacterium, extract the full genome of pure growth, and carry out PCR by 16S rDNA universal primer, identify that bacterial strain is Thiobacillus Thioparaus, called after CYJN-1.
Embodiment 3 bacterial strain Physiology and biochemistries and resistance research
Bacterial strain CYJN-1 is inoculated in by identical inoculum size in the shaking table of differing temps in (10,20,30,40,50 DEG C) liquid nutrient medium, determines that optimum growth temperature is 30 DEG C; Take initial pH3.0,4.0,5.0,6.0,7.0,8.0 and 9.0 to cultivate under optimum temperuture, determine that the most suitable growth pH is 7.0.By the one in following substrate component, determine the best energy substrate (g/L) of bacterial classification CYJN-1: substrate is respectively: yeast extract 1.0; Peptone 1.0; Glucose 1.0; Fructose 1.0; Sucrose 1.0; Lactose 1.0; Semi-lactosi 1.0; Maltose 1.0; Glycine 1.0; Methionin 1.0; Methionine(Met) 1.0; Na
2s
2o
35H
2o 10.0; Na
2s 1.0; S
01.0; Yeast extract 1.0 and Na
2s
2o
35H
2o 10.0; Peptone 1.0 and Na
2s
2o
35H
2o 10.0; Glucose 1.0 and Na
2s
2o
35H
2o 10.0.Determine that CYJN-1 cannot utilize various organism, but can utilize elemental sulfur, sodium sulphite and Sulfothiorine, its best energy substrate is Sulfothiorine.
Embodiment 4 biological desulphurizations
Determining under initial pH, temperature and the concentration of substrate of above-mentioned optimum growh, study the degradation property of this bacterial strain to thiosulfate solution.The substratum of getting the configuration of 100 mL Sulfothiorine is placed in 250 mL Erlenmeyer flasks, and inoculation 5 mL bacterium liquid, cultivate under 170 r/min, and its growth curve and chemical index are as shown in Figure 2.Can find out, just cultivate the lag phase of 12 h inner cells in conforming, the poor growth of bacterial strain, growth curve is horizontal line shape; And in 30 h subsequently, cell enters Exponential growth stage, to grow vigorous, the speed of growth is exceedingly fast, and cell quantity is multiple and increases, and growth curve rises in rain; Afterwards because substratum nutritive substance is limited, and cell quantity is too much, vies each other, so dead cell count and newborn cell count maintain an equal level, enters the stage of stable development.In culturing process, the decline of thiosulfuric acid salt concn is accompanied by the rising of sulfate concentration, this show bacterial strain CYJN-1 taking elemental sulfur as mesostate by thiosulphate-sulphur of divalent is oxidized to vitriol.Batch culture result shows: bacterial strain CYJN-1, under optimum growing condition, can reach 99% to the decreasing ratio of Sulfothiorine within 24 hours.
In sum, of the present invention to have a liking for salt thiobacillus sweetening power higher, is expected to be applied to salinity higher, in the natural desulfurization system of mild condition.
In this specification sheets, the present invention is described with reference to its specific embodiment.But, still can make various amendments and conversion obviously and not deviate from the spirit and scope of the present invention.Therefore, specification sheets and accompanying drawing are regarded in an illustrative, rather than a restrictive.
Claims (3)
- One strain Thiobacillus Thioparaus ( thiobacillus neapolitanus) CYJN-1, being preserved in Chinese Typical Representative culture collection center, deposit number is: CCTCC NO:M 2014129.
- 2. the application of Thiobacillus Thioparaus CCTCC NO:M 2014129 claimed in claim 1, it is characterized in that this bacterial strain cannot utilize various organism, but can utilize sodium sulphite, elemental sulfur and thiosulphate, reduced sulfur compound efficient oxidation is generated to elemental sulfur and vitriol, batch culture shows, this bacterial strain has been realized 99% thiosulphate and has been transformed in 24h, and optimal salinity is that 2%-5%, optimal pH are 7.0, optimum temperuture is 30 DEG C.
- 3. the application of Thiobacillus Thioparaus CCTCC NO:M 2014129 claimed in claim 1, is characterized in that for biological desulphurization.
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| CN103966128A true CN103966128A (en) | 2014-08-06 |
| CN103966128B CN103966128B (en) | 2016-03-23 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105441345A (en) * | 2015-12-21 | 2016-03-30 | 宁波大学 | Candida tropicalis and application thereof to biological desulphurization |
| CN106434486A (en) * | 2016-11-10 | 2017-02-22 | 中国科学院成都生物研究所 | Desulfuration Rhodobacter sp. and application thereof |
| CN106591175A (en) * | 2016-11-10 | 2017-04-26 | 中国科学院成都生物研究所 | Desulfurization Alishewanella aestuarii and applications thereof |
| CN116426440A (en) * | 2023-05-26 | 2023-07-14 | 海南热带海洋学院 | Novel marine sulfur oxidizing bacteria strain and application thereof |
| CN117070424A (en) * | 2023-10-10 | 2023-11-17 | 华南理工大学 | Energy-converting autotrophic thiobacillus haloxydans strain and application thereof |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105441345A (en) * | 2015-12-21 | 2016-03-30 | 宁波大学 | Candida tropicalis and application thereof to biological desulphurization |
| CN105441345B (en) * | 2015-12-21 | 2019-02-22 | 宁波大学 | One plant of Candida tropicalis and its application in biological desulphurization |
| CN106434486A (en) * | 2016-11-10 | 2017-02-22 | 中国科学院成都生物研究所 | Desulfuration Rhodobacter sp. and application thereof |
| CN106591175A (en) * | 2016-11-10 | 2017-04-26 | 中国科学院成都生物研究所 | Desulfurization Alishewanella aestuarii and applications thereof |
| CN106434486B (en) * | 2016-11-10 | 2019-06-14 | 中国科学院成都生物研究所 | The red bacterium of one plant of desulfurization and its application |
| CN106591175B (en) * | 2016-11-10 | 2019-08-23 | 中国科学院成都生物研究所 | One plant of desulfurization Xu not prosperous Salmonella and its application |
| CN116426440A (en) * | 2023-05-26 | 2023-07-14 | 海南热带海洋学院 | Novel marine sulfur oxidizing bacteria strain and application thereof |
| CN116426440B (en) * | 2023-05-26 | 2023-08-18 | 海南热带海洋学院 | Novel marine sulfur oxidizing bacteria strain and application thereof |
| CN117070424A (en) * | 2023-10-10 | 2023-11-17 | 华南理工大学 | Energy-converting autotrophic thiobacillus haloxydans strain and application thereof |
| CN117070424B (en) * | 2023-10-10 | 2023-12-22 | 华南理工大学 | Energy-converting autotrophic thiobacillus haloxydans strain and application thereof |
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| Publication number | Publication date |
|---|---|
| CN103966128B (en) | 2016-03-23 |
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