CN103964928B - The preparation method of microbe soil restoration agent - Google Patents
The preparation method of microbe soil restoration agent Download PDFInfo
- Publication number
- CN103964928B CN103964928B CN201410210911.7A CN201410210911A CN103964928B CN 103964928 B CN103964928 B CN 103964928B CN 201410210911 A CN201410210911 A CN 201410210911A CN 103964928 B CN103964928 B CN 103964928B
- Authority
- CN
- China
- Prior art keywords
- preparation
- restoration agent
- soil restoration
- volume ratio
- microbe soil
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention belongs to the preparation of microbe soil restoration agent, refer to a kind of preparation method of microbe soil restoration agent especially.Comprise and Bacillus licheniformis CGMCC1.7461 made seed liquor through enlarged culturing, containing carbon source, nitrogenous source and somatomedin without aerated culture seed liquor in bacteria fermentation culture medium, obtain and there is the lichen bacillus ferments liquid of organic phosphorus pesticide degradation ability; By the lichen bacillus ferments liquid: liquid microbial Jia Lin composite fertilizer: potassium fulvate concentrated solution makes microbe soil restoration agent after mixture in proportion.The organophosphorus pesticide big area that the invention solves prior art existence uses the problem of environmental pollution caused, there is the security degradation effect of organophosphorus pesticide in soil being improved significantly, effectively to soil fertility structure and food, and the seed output and quality of product all have in various degree put forward advantages of higher.
Description
Technical field
The invention belongs to the preparation of microbe soil restoration agent, refer to a kind of preparation method of microbe soil restoration agent especially.
Background technology
Organophosphorus pesticide causes one of topmost kind of Soil Contamination by Chemical Pesticides.Current organophosphorus pesticide reaches hundreds of, and what use in China about has more than 30 to plant, and wherein more than 80% is highly toxic pesticide, as acephatemet, parathion-methyl, thiophos, monocrotophos etc.Forbidden or limit the use of by many countries because above-mentioned organophosphorus pesticide has severe toxicity, but because such agricultural insecticide is effective, instant effect and low price, the situation that China's organophosphorus pesticide occupies agricultural chemicals dominant position is difficult to change in a short time, and the problem of environmental pollution caused thus becomes increasingly conspicuous.
Pesticide residue beyond natural ecosystems self repair ability, seek the method for degrading organic phosphor pesticides, and eliminating its pollution caused becomes letter problem to be solved, in the urgent need to there being practicable pesticide residue removal technology.Microorganism all has good Degradation to the organophosphorus pesticide in soil and water, and can not secondary pollution be caused, found at present the microorganism of degrading organic phosphor to have a variety of, but utilizing fermentation engineering to produce microorganism live bacteria preparation rarely has report at home for the reparation of contaminated soil.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of microbe soil restoration agent, can realize improving edatope by beneficial microorganism physiological metabolism in microbe soil restoration agent, degrading organic phosphor pesticides remains, improve crop to the utilization of fertilizer, the object that increases soil nutrient, improve crop yield and quality.
Overall technology design of the present invention is:
The preparation method of microbe soil restoration agent, comprises following processing step:
A, Bacillus licheniformis CGMCC1.7461 is made seed liquor through enlarged culturing;
B, containing carbon source, nitrogenous source and somatomedin without aerated culture seed liquor in bacteria fermentation culture medium, obtain and there is the lichen bacillus ferments liquid of organic phosphorus pesticide degradation ability;
C, according to the lichen bacillus ferments liquid: liquid microbial Jia Lin composite fertilizer: volume ratio=10-50: 10-50: the 1-10 of potassium fulvate concentrated solution ratio mixing after make microbe soil restoration agent; Wherein liquid microbial Jia Lin composite fertilizer is selected from operative norm GB20287-2006, and the effective bacterial content of microorganism is not less than the commercially available prod of 200,000,000/milliliter.Wherein preferred technical scheme is, " Ji Wei " board biological clamps phosphate fertilizer (composite fungus agent) that Hebei Institute of Microbiology produces, the universal microbial inoculum of " Yuyuan Garden " board that Hunan Yuyuan Biology Technology Co., Ltd produces, " A Musi " board biological phosphorus potash fertilizer etc. that Beijing Century Aims Biotechnology Co., Ltd produces, wherein highly preferred technical scheme is, " Ji Wei " board biological clamps phosphate fertilizer (composite fungus agent) that Hebei Institute of Microbiology produces.
The Bacillus licheniformis CGMCC1.7461 that the present invention selects in laboratory conditions, in 24h, more than 85% is reached to 50mg/L methamidophos degradation rate, in 8h, more than 90% is reached to 50mg/L parathion-methyl degradation rate, can Partial digestion Volaton, Rogor (omethoate).
Concrete technology contents of the present invention also has:
In described step C in the microbe soil restoration agent made according to liquid bio potash fertilizer protective material: the ratio of volume ratio=0.2-1: 100 of microbe soil restoration agent adds liquid bio potash fertilizer protective material.
Described liquid bio potash fertilizer protective material is selected from the patent documentation of publication number CN102838416A.
Enlarged culturing in described steps A comprises the preparation of Kolle flask bacterial classification and the preparation of seed liquor.
The preparation of described Kolle flask seed is seeded in aseptic Kolle flask substratum by the original strain in test tube slant to make Kolle flask bacterial classification through cultivation, and wherein test tube slant and Kolle flask substratum are made up of the component of following mass percentage:
Extractum carnis 0.1%-0.5%, peptone 0.5%-2.0%, sodium-chlor 0.3%-1.0%, manganous sulfate 0.001%-0.003%, agar 1.8%-2.3%, surplus is water, pH=7.0-7.2;
The culture condition of test tube slant and Kolle flask bacterial classification is: temperature is 30-35 DEG C, time 1-3 days.
The preparation of described seed liquor is that Kolle flask strain inoculation is made seed liquor by fermentation to aseptic seed culture medium, and wherein seed culture medium is made up of the component of following mass percentage:
Starch 0.1%-0.5%, sucrose 0.3%-1.0%, manganous sulfate 0.001%-0.003%, dipotassium hydrogen phosphate 0.1%-0.5%, potassium primary phosphate 0.1%-0.2%, soybean cake powder 1.0%-5.0%, surplus is water, pH=7.0-7.5;
The fermentation condition of seed liquor is: be the ratio loading amount of 0.55: 1 according to volume ratio, inoculum size is according to Kolle flask bacterial classification: volume ratio=0.03-0.1: 1 of seed culture medium, and culture temperature is 30-31 DEG C; Tank pressure is 0.05Mpa; Ventilation is according to seed culture medium: volume ratio=1 of air quantity: 0.3-0.8; Fermentation period is 18-24 hour.
In described step B, fermention medium is made up of the component of following mass percentage:
Starch 0.1%-0.5%, sucrose 0.3%-1.0%, manganous sulfate 0.001%-0.003%, dipotassium hydrogen phosphate 0.1%-0.5%, potassium primary phosphate 0.1%-0.2%, soybean cake powder 1.0%-5.0%, surplus is water, pH=7.0-7.5;
The condition of aerated culture is: be the ratio loading amount of 0.55: 1 according to volume ratio, inoculum size is according to seed liquor: volume ratio=0.03-0.1: 1 of fermention medium, and culture temperature is 30 DEG C-31 DEG C; Tank pressure is 0.05Mpa; Ventilation is according to fermention medium: volume ratio=1 of air quantity: 0.3-0.8; Fermentation period is 36-48 hour.
Fermentation termination in described step B adopts microscopical determination counting, when fermented liquid without miscellaneous bacteria, more than 95% thalline forms gemma, spore content reaches 6,500,000,000/more than ml.
The sterilising conditions of the seed culture medium in described seed liquor preparation and the fermention medium in aerated culture is yes: temperature is 121 DEG C; Pressure is 0.1Mpa.
Substantive distinguishing features acquired by the present invention and significant technical progress are:
The microbe soil restoration agent adopting the method in the present invention to prepare is tried out through applicant, proves the degraded of organophosphorus pesticide in soil very effective, particularly for growing vegetables base and the industrialized agriculture of specialty, and Be very effective.By the consumption of every mu of 5-10L in growing vegetables, as base manure, gradation or disposable employed of topdressing, the pesticide residue for polluted soil and product all can reach standard-required, effectively improve the security of soil fertility structure and food.Use this soil-repairing agent planting vegetable in detection organophosphorus pesticide exceeds standard 50-100 soil doubly after, detection soil and product organophosphorus pesticide all do not exceed standard or do not detect (detection method and characteristic evidences GB2763-2012, NYT761-2008), and the seed output and quality of product all has raising in various degree.
Embodiment
Below in conjunction with embodiment, the present invention is described further; but it is not as a limitation of the invention; the content that protection scope of the present invention is recorded with claim is as the criterion, and any equivalent technical elements made according to specification sheets is replaced, and does not all depart from protection scope of the present invention.
Embodiment 1
The preparation method of microbe soil restoration agent, comprises following processing step:
A, Bacillus licheniformis CGMCC1.7461 is made seed liquor through enlarged culturing;
B, containing carbon source, nitrogenous source and somatomedin without aerated culture seed liquor in bacteria fermentation culture medium, obtain and there is the lichen bacillus ferments liquid of organic phosphorus pesticide degradation ability;
C, according to the lichen bacillus ferments liquid: liquid microbial Jia Lin composite fertilizer: volume ratio=10 of potassium fulvate concentrated solution: 10: 1 ratio mixing after make microbe soil restoration agent; Wherein liquid microbial Jia Lin composite fertilizer is selected from operative norm GB20287-2006, and the effective bacterial content of microorganism is not less than the commercially available prod of 200,000,000/milliliter." Ji Wei " board biological clamps phosphate fertilizer (composite fungus agent) that preferred employing Hebei Institute of Microbiology produces.
In described step C in the microbe soil restoration agent made according to liquid bio potash fertilizer protective material: volume ratio=0.2 of microbe soil restoration agent: the ratio of 100 adds liquid bio potash fertilizer protective material.Described liquid bio potash fertilizer protective material is selected from the patent documentation of publication number CN102838416A.
Enlarged culturing in described steps A comprises the preparation of Kolle flask bacterial classification and the preparation of seed liquor.
The preparation of described Kolle flask seed is seeded in aseptic Kolle flask substratum by the original strain in test tube slant to make Kolle flask bacterial classification through cultivation, and wherein test tube slant and Kolle flask substratum are made up of the component of following mass percentage:
Extractum carnis 0.1%, peptone 0.5%, sodium-chlor 0.3%, manganous sulfate 0.001%, agar 1.8%, surplus is water, pH=7.0-7.2;
The culture condition of test tube slant and Kolle flask bacterial classification is: temperature is 30 DEG C, 3 days time.
The preparation of described seed liquor is that Kolle flask strain inoculation is made seed liquor by fermentation to aseptic seed culture medium, and wherein seed culture medium is made up of the component of following mass percentage:
Starch 0.1%, sucrose 0.3%, manganous sulfate 0.001%, dipotassium hydrogen phosphate 0.1%, potassium primary phosphate 0.1%, soybean cake powder 1.0%, surplus is water, pH=7.0-7.5;
The fermentation condition of seed liquor is: be the ratio loading amount of 0.55: 1 according to volume ratio, inoculum size is according to Kolle flask bacterial classification: volume ratio=0.03 of seed culture medium: 1, and culture temperature is 30 DEG C; Tank pressure is 0.05Mpa; Ventilation is according to seed culture medium: volume ratio=1 of air quantity: 0.3; Fermentation period is 24 hours.
In described step B, fermention medium is made up of the component of following mass percentage:
Starch 0.1%, sucrose 0.3%, manganous sulfate 0.001%, dipotassium hydrogen phosphate 0.1%, potassium primary phosphate 0.1%, soybean cake powder 1.0%, surplus is water, pH=7.0-7.5;
The condition of aerated culture is: be the ratio loading amount of 0.55: 1 according to volume ratio, inoculum size is according to seed liquor: volume ratio=0.03 of fermention medium: 1, and culture temperature is 30 DEG C; Tank pressure is 0.05Mpa; Ventilation is according to fermention medium: volume ratio=1 of air quantity: 0.3; Fermentation period is 48 hours.
Fermentation termination in described step B be adopt microscopical determination and counting when fermented liquid without miscellaneous bacteria, more than 95% thalline form gemma, spore content and reach 6,500,000,000/more than ml.
The sterilising conditions of the seed culture medium in described seed liquor preparation and the fermention medium in aerated culture is yes: temperature is 121 DEG C; Pressure is 0.1Mpa.
Embodiment 2
The difference of the present embodiment and embodiment 1 is:
According to the lichen bacillus ferments liquid in step C: liquid microbial Jia Lin composite fertilizer: volume ratio=50 of potassium fulvate concentrated solution: make microbe soil restoration agent after the ratio mixing of 50: 10; Wherein liquid microbial Jia Lin composite fertilizer is selected from operative norm GB20287-2006, and the effective bacterial content of microorganism is not less than the commercially available prod of 200,000,000/milliliter.Adopt the universal microbial inoculum of " Yuyuan Garden " board that Hunan Yuyuan Biology Technology Co., Ltd produces.
The preparation of described Kolle flask seed is seeded in aseptic Kolle flask substratum by the original strain in test tube slant to make Kolle flask bacterial classification through cultivation, and wherein test tube slant and Kolle flask substratum are made up of the component of following mass percentage:
Extractum carnis 0.5%, peptone 2.0%, sodium-chlor 1.0%, manganous sulfate 0.003%, agar 2.3%, surplus is water, pH=7.0-7.2;
The culture condition of test tube slant and Kolle flask bacterial classification is: temperature is 35 DEG C, 1 day time.
The preparation of described seed liquor is that Kolle flask strain inoculation is made seed liquor by fermentation to aseptic seed culture medium, and wherein seed culture medium is made up of the component of following mass percentage:
Starch 0.5%, sucrose 1.0%, manganous sulfate 0.003%, dipotassium hydrogen phosphate 0.5%, potassium primary phosphate 0.2%, soybean cake powder 5.0%, surplus is water, pH=7.0-7.5;
The fermentation condition of seed liquor is: be the ratio loading amount of 0.55: 1 according to volume ratio, inoculum size is according to Kolle flask bacterial classification: volume ratio=0.1 of seed culture medium: 1, and culture temperature is 31 DEG C; Tank pressure is 0.05Mpa; Ventilation is according to seed culture medium: volume ratio=1 of air quantity: 0.8; Fermentation period is 18 hours.
In described step B, fermention medium is made up of the component of following mass percentage:
Starch 0.5%, sucrose 1.0%, manganous sulfate 0.003%, dipotassium hydrogen phosphate 0.5%, potassium primary phosphate 0.2%, soybean cake powder 5.0%, surplus is water, pH=7.0-7.5;
The condition of aerated culture is: be the ratio loading amount of 0.55: 1 according to volume ratio, inoculum size is according to seed liquor: volume ratio=0.1 of fermention medium: 1, and culture temperature is 31 DEG C; Tank pressure is 0.05Mpa; Ventilation is according to fermention medium: volume ratio=1 of air quantity: 0.8; Fermentation period is 36 hours.
All the other contents are with embodiment 1.
Embodiment 3
According to the lichen bacillus ferments liquid in step C: liquid microbial Jia Lin composite fertilizer: volume ratio=30 of potassium fulvate concentrated solution: make microbe soil restoration agent after the ratio mixing of 30: 5; Wherein liquid microbial Jia Lin composite fertilizer is selected from operative norm GB20287-2006, and the effective bacterial content of microorganism is not less than the commercially available prod of 200,000,000/milliliter." A Musi " board biological phosphorus potash fertilizer selecting Beijing Century Aims Biotechnology Co., Ltd to produce.
In described step C in the microbe soil restoration agent made according to liquid bio potash fertilizer protective material: volume ratio=0.6 of microbe soil restoration agent: the ratio of 100 adds liquid bio potash fertilizer protective material, described liquid bio potash fertilizer protective material is selected from the patent documentation of publication number CN102838416A.
The preparation of described Kolle flask seed is seeded in aseptic Kolle flask substratum by the original strain in test tube slant to make Kolle flask bacterial classification through cultivation, and wherein test tube slant and Kolle flask substratum are made up of the component of following mass percentage:
Extractum carnis 0.3%, peptone 1%, sodium-chlor 0.6%, manganous sulfate 0.002%, agar 2%, surplus is water, pH=7.0-7.2;
The culture condition of test tube slant and Kolle flask bacterial classification is: temperature is 33 DEG C, 2 days time.
Seed culture medium is made up of the component of following mass percentage:
Starch 0.3%, sucrose 0.6%, manganous sulfate 0.002%, dipotassium hydrogen phosphate 0.3%, potassium primary phosphate 0.15%, soybean cake powder 3%, surplus is water, pH=7.0-7.5;
The fermentation condition of seed liquor is: be the ratio loading amount of 0.55: 1 according to volume ratio, inoculum size is according to Kolle flask bacterial classification: volume ratio=0.06 of seed culture medium: 1, and culture temperature is 30 DEG C; Tank pressure is 0.05Mpa; Ventilation is according to seed culture medium: volume ratio=1 of air quantity: 0.5; Fermentation period is 20 hours.
In described step B, fermention medium is made up of the component of following mass percentage:
Starch 0.3%, sucrose 0.6%, manganous sulfate 0.002%, dipotassium hydrogen phosphate 0.3%, potassium primary phosphate 0.15%, soybean cake powder 3%, surplus is water, pH=7.0-7.5;
The condition of aerated culture is: be the ratio loading amount of 0.55: 1 according to volume ratio, inoculum size is according to seed liquor: volume ratio=0.06 of fermention medium: 1, and culture temperature is 30 DEG C; Tank pressure is 0.05Mpa; Ventilation is according to fermention medium: volume ratio=1 of air quantity: 0.6; Fermentation period is 40 hours.
All the other contents are with embodiment 1.
Embodiment 4
The difference of the present embodiment and embodiment 1 is:
According to the lichen bacillus ferments liquid in step C: liquid microbial Jia Lin composite fertilizer: volume ratio=10 of potassium fulvate concentrated solution: make microbe soil restoration agent after the ratio mixing of 50: 8.
Enlarged culturing in described steps A comprises the preparation of Kolle flask bacterial classification and the preparation of seed liquor.
The preparation of described Kolle flask seed is seeded in aseptic Kolle flask substratum by the original strain in test tube slant to make Kolle flask bacterial classification through cultivation, and wherein test tube slant and Kolle flask substratum are made up of the component of following mass percentage:
Extractum carnis 0.1%, peptone 1.5%, sodium-chlor 1.0%, manganous sulfate 0.003%, agar 2%, surplus is water, pH=7.0-7.2;
The culture condition of test tube slant and Kolle flask bacterial classification is: temperature is 33 DEG C, 2 days time.
The preparation of described seed liquor is that Kolle flask strain inoculation is made seed liquor by fermentation to aseptic seed culture medium, and wherein seed culture medium is made up of the component of following mass percentage:
Starch 0.1%, sucrose 1.0%, manganous sulfate 0.003%, dipotassium hydrogen phosphate 0.4%, potassium primary phosphate 0.2%, soybean cake powder 4%, surplus is water, pH=7.0-7.5;
The fermentation condition of seed liquor is: be the ratio loading amount of 0.55: 1 according to volume ratio, inoculum size is according to Kolle flask bacterial classification: volume ratio=0.08 of seed culture medium: 1, and culture temperature is 30-31 DEG C; Tank pressure is 0.05Mpa; Ventilation is according to seed culture medium: volume ratio=1 of air quantity: 0.4; Fermentation period is 18-24 hour.
In described step B, fermention medium is made up of the component of following mass percentage:
Starch 0.3%, sucrose 0.8%, manganous sulfate 0.002%, dipotassium hydrogen phosphate 0.3%, potassium primary phosphate 0.15%, soybean cake powder 3%, surplus is water, pH=7.0-7.5;
The condition of aerated culture is: be the ratio loading amount of 0.55: 1 according to volume ratio, inoculum size is according to seed liquor: volume ratio=0.08 of fermention medium: 1, and culture temperature is 30 DEG C-31 DEG C; Tank pressure is 0.05Mpa; Ventilation is according to fermention medium: volume ratio=1 of air quantity: 0.5; Fermentation period is 36-48 hour.
All the other contents are with embodiment 1.
Embodiment 5
The difference of the present embodiment and embodiment 1 is:
According to the lichen bacillus ferments liquid in step C: liquid microbial Jia Lin composite fertilizer: volume ratio=30 of potassium fulvate concentrated solution: make microbe soil restoration agent after the ratio mixing of 20: 10.
In described step C in the microbe soil restoration agent made according to liquid bio potash fertilizer protective material: volume ratio=1 of microbe soil restoration agent: the ratio of 100 adds liquid bio potash fertilizer protective material.
Enlarged culturing in described steps A comprises the preparation of Kolle flask bacterial classification and the preparation of seed liquor.
The preparation of described Kolle flask seed is seeded in aseptic Kolle flask substratum by the original strain in test tube slant to make Kolle flask bacterial classification through cultivation, and wherein test tube slant and Kolle flask substratum are made up of the component of following mass percentage:
Extractum carnis 0.5%, peptone 2.0%, sodium-chlor 1.0%, manganous sulfate 0.003%, agar 1.8%, surplus is water, pH=7.0-7.2;
The culture condition of test tube slant and Kolle flask bacterial classification is: temperature is 32 DEG C, 3 days time.
The preparation of described seed liquor is that Kolle flask strain inoculation is made seed liquor by fermentation to aseptic seed culture medium, and wherein seed culture medium is made up of the component of following mass percentage:
Starch 0.3%, sucrose 0.8%, manganous sulfate 0.002%, dipotassium hydrogen phosphate 0.3%, potassium primary phosphate 0.15%, soybean cake powder 4%, surplus is water, pH=7.0-7.5;
The fermentation condition of seed liquor is: be the ratio loading amount of 0.55: 1 according to volume ratio, inoculum size is according to Kolle flask bacterial classification: volume ratio=0.08 of seed culture medium: 1, and culture temperature is 30-31 DEG C; Tank pressure is 0.05Mpa; Ventilation is according to seed culture medium: volume ratio=1 of air quantity: 0.5; Fermentation period is 18-24 hour.
In described step B, fermention medium is made up of the component of following mass percentage:
Starch 0.4%, sucrose 0.8%, manganous sulfate 0.002%, dipotassium hydrogen phosphate 0.4%, potassium primary phosphate 0.15%, soybean cake powder 3%, surplus is water, pH=7.0-7.5;
The condition of aerated culture is: be the ratio loading amount of 0.55: 1 according to volume ratio, inoculum size is according to seed liquor: volume ratio=0.06 of fermention medium: 1, and culture temperature is 30 DEG C-31 DEG C; Tank pressure is 0.05Mpa; Ventilation is according to fermention medium: volume ratio=1 of air quantity: 0.5; Fermentation period is 36-48 hour.
All the other contents are with embodiment 1.
Applicant has carried out following test in Xushui vegetables cooperative society to cucumber by according to product prepared in embodiment 1-5:
Select before plantation by the more serious soil of pesticidal contamination, agricultural chemicals residual after measured on average exceeds standard 58 times.Take conventional fertilizer application as control group; On the basis of conventional fertilizer application, this soil-repairing agent 10L is used in increase is test group (dip in root when soil-repairing agent 3L transplants to use, 7L fills with root when topdressing in addition or punching is used).Ordinary method field management, topdresses, weeding, laxative etc. is all identical.Sowing on April 20 (in cloudy canopy), May 23 transplanted, and started to adopt melon at the beginning of 6 months.
Test-results is as following table.
Test-results is as follows
Vegetative period observes: control group growth is normal; Test group is emerged neat healthy and strong, and stem is climing sturdy, and blade is plump, and percentage of fertile fruit is high, and without disease and pest, sugar-preserved gourd is straight, and curcumbitate is good, and mouthfeel is good.
Yield compari: control group per mu yield 5298 kilograms, test group per mu yield is respectively 6718,6629,6775,6439,6342 kilograms, do not increase production 1420,1331,1477,1141,1044 kilograms than contrast component, stimulation ratio reaches 26.8%, 25.1%, 27.9%, 21.5%, 19.7% respectively.
Pesticide residue compare: the pesticide residue detecting cucumber after adopting melon, adopt the pesticide residue that melon terminates in rear detection soil.Control group cucumber has the recall rate of 30%, but does not exceed standard; Test group does not detect.Control group Soil K+adsorption no significant difference front with plantation; Test group Pesticide-Polluted Soil recall rate is respectively 4.97%, 4.23%, 3.56%, 1.67%, 0.87%, does not all exceed standard.
Claims (9)
1. the preparation method of microbe soil restoration agent, is characterized in that comprising following processing step:
A, Bacillus licheniformis CGMCC1.7461 is made seed liquor through enlarged culturing;
B, containing carbon source, nitrogenous source and somatomedin without aerated culture seed liquor in bacteria fermentation culture medium, obtain and there is the lichen bacillus ferments liquid of organic phosphorus pesticide degradation ability;
C, according to the lichen bacillus ferments liquid: liquid microbial Jia Lin composite fertilizer: volume ratio=10-50: 10-50: the 1-10 of potassium fulvate concentrated solution ratio mixing after make microbe soil restoration agent; Wherein liquid microbial Jia Lin composite fertilizer is selected from operative norm GB20287-2006, and the effective bacterial content of microorganism is not less than the commercially available prod of 200,000,000/milliliter.
2. the preparation method of microbe soil restoration agent according to claim 1, to is characterized in that in described step C in the microbe soil restoration agent made according to liquid bio potash fertilizer protective material: the ratio of volume ratio=0.2-1: 100 of microbe soil restoration agent adds liquid bio potash fertilizer protective material.
3. the preparation method of microbe soil restoration agent according to claim 2, is characterized in that described liquid bio potash fertilizer protective material is selected from the patent documentation of publication number CN102838416A.
4. the preparation method of microbe soil restoration agent according to claim 1, is characterized in that the enlarged culturing in described steps A comprises the preparation of Kolle flask bacterial classification and the preparation of seed liquor.
5. the preparation method of microbe soil restoration agent according to claim 4, it is characterized in that the preparation of described Kolle flask seed is seeded in aseptic Kolle flask substratum by the original strain in test tube slant to make Kolle flask bacterial classification through cultivation, wherein test tube slant and Kolle flask substratum are made up of the component of following mass percentage:
Extractum carnis 0.1%-0.5%, peptone 0.5%-2.0%, sodium-chlor 0.3%-1.0%, manganous sulfate 0.001%-0.003%, agar 1.8%-2.3%, surplus is water, pH=7.0-7.2;
The culture condition of test tube slant and Kolle flask bacterial classification is: temperature is 30-35 DEG C, time 1-3 days.
6. the preparation method of the microbe soil restoration agent according to any one of claim 4 or 5, it is characterized in that the preparation of described seed liquor is that Kolle flask strain inoculation is made seed liquor by fermentation to aseptic seed culture medium, wherein seed culture medium is made up of the component of following mass percentage:
Starch 0.1%-0.5%, sucrose 0.3%-1.0%, manganous sulfate 0.001%-0.003%, dipotassium hydrogen phosphate 0.1%-0.5%, potassium primary phosphate 0.1%-0.2%, soybean cake powder 1.0%-5.0%, surplus is water, pH=7.0-7.5;
The fermentation condition of seed liquor is: be the ratio loading amount of 0.55: 1 according to volume ratio, inoculum size is according to Kolle flask bacterial classification: volume ratio=0.03-0.1: 1 of seed culture medium, and culture temperature is 30-31 DEG C; Tank pressure is 0.05Mpa; Ventilation is according to seed culture medium: volume ratio=1 of air quantity: 0.3-0.8; Fermentation period is 18-24 hour.
7. the preparation method of microbe soil restoration agent according to claim 1, is characterized in that in described step B, fermention medium is made up of the component of following mass percentage:
Starch 0.1%-0.5%, sucrose 0.3%-1.0%, manganous sulfate 0.001%-0.003%, dipotassium hydrogen phosphate 0.1%-0.5%, potassium primary phosphate 0.1%-0.2%, soybean cake powder 1.0%-5.0%, surplus is water, pH=7.0-7.5;
The condition of aerated culture is: be the ratio loading amount of 0.55: 1 according to volume ratio, inoculum size is according to seed liquor: volume ratio=0.03-0.1: 1 of fermention medium, and culture temperature is 30 DEG C-31 DEG C; Tank pressure is 0.05Mpa; Ventilation is according to fermention medium: volume ratio=1 of air quantity: 0.3-0.8; Fermentation period is 36-48 hour.
8. the preparation method of microbe soil restoration agent according to claim 7, the fermentation termination that it is characterized in that in described step B be adopt microscopical determination and counting when fermented liquid without miscellaneous bacteria, more than 95% thalline forms gemma, spore content reaches 6,500,000,000/more than ml.
9. the preparation method of the microbe soil restoration agent according to any one of claim 1,4,7, is characterized in that the sterilising conditions of the seed culture medium in described seed liquor preparation and the fermention medium in aerated culture is yes: temperature is 121 DEG C; Pressure is 0.1Mpa.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410210911.7A CN103964928B (en) | 2014-05-19 | 2014-05-19 | The preparation method of microbe soil restoration agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410210911.7A CN103964928B (en) | 2014-05-19 | 2014-05-19 | The preparation method of microbe soil restoration agent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103964928A CN103964928A (en) | 2014-08-06 |
| CN103964928B true CN103964928B (en) | 2015-11-18 |
Family
ID=51234991
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410210911.7A Active CN103964928B (en) | 2014-05-19 | 2014-05-19 | The preparation method of microbe soil restoration agent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103964928B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105859357A (en) * | 2016-05-04 | 2016-08-17 | 北京科技大学 | Method for preparing microbial fertilizer through by-products generated in bean vermicelli production process |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105950172A (en) * | 2016-06-17 | 2016-09-21 | 战锡林 | Organophosphorus pesticide contaminated soil remediation agent |
| CN108753579A (en) * | 2018-07-11 | 2018-11-06 | 淮阴师范学院 | A kind of microbe soil restoration agent preparation method and its equipment |
| CN109234260B (en) * | 2018-11-23 | 2019-07-23 | 广东省中药研究所 | A kind of preparation method of high-activity microorganism soil-repairing agent |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101210182A (en) * | 2006-12-25 | 2008-07-02 | 山东泉林纸业有限责任公司 | Method for producing soil restoring agent by using straw slurry black liquor |
| CN102505010A (en) * | 2011-10-21 | 2012-06-20 | 上海交通大学 | Preparation method of trichoderma multifunctional soil modifying agents |
| CN102653754A (en) * | 2012-05-29 | 2012-09-05 | 中华人民共和国沈阳出入境检验检疫局 | Preparation method of microorganism immobilization particles for removing residual pesticide in soil |
| CN102786934A (en) * | 2012-08-22 | 2012-11-21 | 王金玲 | Probiotic soil conditioner and production method thereof |
| CN102838416A (en) * | 2012-09-19 | 2012-12-26 | 河北省微生物研究所 | Liquid biological potassium fertilizer protecting agent |
| CN103555624A (en) * | 2013-10-25 | 2014-02-05 | 王天喜 | Nitrogen fixation bacillus licheniformis and application thereof |
| CN103708973A (en) * | 2014-01-10 | 2014-04-09 | 长沙新源氨基酸生物肥料有限公司 | Preparation method, product and application of soil remediating active organic fertilizer |
-
2014
- 2014-05-19 CN CN201410210911.7A patent/CN103964928B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101210182A (en) * | 2006-12-25 | 2008-07-02 | 山东泉林纸业有限责任公司 | Method for producing soil restoring agent by using straw slurry black liquor |
| CN102505010A (en) * | 2011-10-21 | 2012-06-20 | 上海交通大学 | Preparation method of trichoderma multifunctional soil modifying agents |
| CN102653754A (en) * | 2012-05-29 | 2012-09-05 | 中华人民共和国沈阳出入境检验检疫局 | Preparation method of microorganism immobilization particles for removing residual pesticide in soil |
| CN102786934A (en) * | 2012-08-22 | 2012-11-21 | 王金玲 | Probiotic soil conditioner and production method thereof |
| CN102838416A (en) * | 2012-09-19 | 2012-12-26 | 河北省微生物研究所 | Liquid biological potassium fertilizer protecting agent |
| CN103555624A (en) * | 2013-10-25 | 2014-02-05 | 王天喜 | Nitrogen fixation bacillus licheniformis and application thereof |
| CN103708973A (en) * | 2014-01-10 | 2014-04-09 | 长沙新源氨基酸生物肥料有限公司 | Preparation method, product and application of soil remediating active organic fertilizer |
Non-Patent Citations (2)
| Title |
|---|
| 有机磷农药广谱活性降解菌的分离及其生理特性研究;王永杰等;《南京农业大学学报》;19990530(第2期);第42-45页 * |
| 有机磷农药降解菌的紫外诱变育种;王永杰等;《应用与环境生物学报》;19991225;第5卷(第6期);第635-637页 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105859357A (en) * | 2016-05-04 | 2016-08-17 | 北京科技大学 | Method for preparing microbial fertilizer through by-products generated in bean vermicelli production process |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103964928A (en) | 2014-08-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104789490B (en) | A kind of complex microorganism decomposing microbial inoculum, preparation method and application | |
| CN104447095B (en) | A kind of biological activity organic medical fertilizer and its preparation method and application | |
| CN101717315B (en) | Special peanut organic and inorganic compound fertilizer for biologically preventing and treating root knot nematode disease and preparation method thereof | |
| CN102154194B (en) | Preparation method for high-yield chlamydospore liquid fermentation from trichoderma on pilot plant test scale | |
| CN111807898A (en) | Bio-organic fertilizer with function of preventing and treating tobacco soil-borne diseases and preparation method thereof | |
| CN103937700A (en) | Composite biological agent used for restoring cadmium polluted soil, and its preparation method | |
| CN103203356A (en) | Secondarily-salinized soil microbial agent and preparation and application thereof | |
| CN103964928B (en) | The preparation method of microbe soil restoration agent | |
| CN104744182A (en) | Tobacco water retention biological organic fertilizer and preparation method thereof | |
| CN103194410A (en) | Paenibacillus mucilaginosus and method for producing compound microorganism bacterium agent by utilizing same | |
| CN105198509A (en) | Special biological organic fertilizer for cotton and preparation method of special biological organic fertilizer | |
| CN103224904A (en) | Rhodopseudomonas strain, biocontrol microbial inoculum and biocontrol fermentation liquid, and corresponding preparation methods and application thereof in controlling phytophthora blight of pepper | |
| CN105462881A (en) | Paenibacillus polymyxa for preventing and treating vertieillium wilt in crops and application of paenibacillus polymyxa | |
| CN113321547A (en) | Rhizosphere growth promoting water-soluble microbial fertilizer and preparation method thereof | |
| CN105543149B (en) | One plant of new bacillus megaterium and its application | |
| CN102424635B (en) | Preparation method for composite continuous cropping resistant agent specially used for strawberries | |
| CN101935627B (en) | Bromoxynil octanoate degrading bacteria and bacterial agent prepared from same | |
| CN101659570A (en) | Preparation method of biofertilizer | |
| CN104860732A (en) | Microbial strain organic fertilizer for improving soil alkalinity | |
| CN102531746A (en) | Biological fertilizer prepared through one time mixing fermentation by using straws and chemical fertilizer, and preparation method thereof | |
| CN104152373B (en) | Bacterial strain capable of efficiently degrading pendimethalin and application thereof | |
| CN113736661A (en) | Screening method of salt-tolerant growth-promoting rhizobacteria, strain and application thereof | |
| CN105420172A (en) | Metribuzin pesticide residue degrading bacteria, microbial agent produced through same and application of metribuzin pesticide residue degrading bacteria | |
| CN104498041A (en) | Application of peoriae paenibacillus as soil ecology improvement repairing agent | |
| CN115011486B (en) | Paecilomyces lilacinus and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20161017 Address after: 050000 Hebei, Shijiazhuang Bridge West Zhongshan Road, building No. 166, No. 2 Patentee after: Hebei great beauty Environmental Remediation Technology Co., Ltd. Address before: 071051 No. 54 Middle Road, Hebei, Baoding, China, 2089 Patentee before: Hebei Research Institute of Microbiology |
|
| CP03 | Change of name, title or address |
Address after: 050000 Hebei City, Shijiazhuang Province Bridge West Zhongshan Road Ruyi Business Building, No. 26, layer 166 Patentee after: Hebei great beauty environmental remediation Polytron Technologies Inc Address before: 050000 Hebei, Shijiazhuang Bridge West Zhongshan Road, building No. 166, No. 2 Patentee before: Hebei great beauty Environmental Remediation Technology Co., Ltd. |
|
| CP03 | Change of name, title or address |