CN103951084A - Preparation and application of immobilized straight-tubular bioactive filler based on reticulate carrier - Google Patents
Preparation and application of immobilized straight-tubular bioactive filler based on reticulate carrier Download PDFInfo
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- CN103951084A CN103951084A CN201410137349.XA CN201410137349A CN103951084A CN 103951084 A CN103951084 A CN 103951084A CN 201410137349 A CN201410137349 A CN 201410137349A CN 103951084 A CN103951084 A CN 103951084A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
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Abstract
The invention discloses preparation and an application of an immobilized straight-tubular bioactive filler based on a reticulate carrier, and belongs to the technical field of water treatment. The straight-tubular bioactive filler comprises two parts: an embedding body and the carrier; the carrier is a straight-tubular carrier formed by taking polyethylene or polypropylene as a main material, adding a polyvinyl alcohol hydrophilic material and carrying out hot melting or plate hot pressing; with a reticulate structure of the carrier, the embedding body can be allowed to penetrate through meshes to form a riveting fixing structure together with the carrier so as to increase the filler overall stability; an embedding liquid is formed by mixing a bacteria concentrated solution and a polyvinyl alcohol solution; the reticulate carrier is evenly coated with the embedding liquid, and the embedding body is formed after boric acid secondary cross-linking of the embedding liquid and is combined with the reticulate carrier to obtain the bioactive filler. The prepared immobilized straight-tubular bioactive filler not only solves the problems that bacteria are difficult in advantage establishment, easy to lose and the like, but also improves the processing ability of a reactor, shortens the start-up time, and has broad application prospects.
Description
Technical field
The invention belongs to water treatment field, particularly a kind of preparation of immobilization straight-tube shape biological active filling material and application based on mesh carrier.
Background technology
Microorganism cells immobilization technology can increase substantially microorganism concn, making microorganism be difficult for loss, shortening reactor start-up time, toxin immunity and acid and alkali-resistance, salt resistance ability obviously strengthens, the ecological dominance of energy purifying and creation characteristic bacterium, produce characteristic highly effective reaction, demonstrate good application prospect.The process for fixation that microorganism cells is conventional has absorption method, crosslinking and entrapping method.Wherein, the most conventional with entrapping method, the material that has been successfully used to microorganism cells embedding has: polyvinyl alcohol, agar, K-carrageenin, gelatin, sodium alginate, polyacrylamide, urethane etc.That above-mentioned embedded material has is good to microorganism nontoxicity, mass-transfer performance, be difficult for by biological decomposition, stable in properties, the feature such as physical strength is high, the life-span is long, price is low.
At present, traditional bacterium embedding method is that bacterium and embedded material are combined, and forms embedding body, such as microballoon, embedded block etc.This method is simple to operate, but limitation is large, cannot ideal application in the middle of engineering reality.We,, through research for many years, improve on traditional embedding method, and embedding body and carrier are combined, and form overall biological active filling material.Due to the combination of embedding body and carrier, make biological active filling material there is more performance, it has inherited all advantages of original simple bacterium and embedded material; Because making biological active filling material, the application of solid support material there is the characteristic that various carriers have, such as the advantage such as easy mobility, large, the difficult obstruction of specific surface area; The various forms of biological active filling materials that also can make as required can floated, precipitable formula, floatability formula or region " fixed " etc. have other function.Experimental study shows, utilizing the conventional carriers such as existing Raschig ring, Pall ring, cascade ring, suspension modified filler and embedding body to combine by adhesion mode the biological active filling material making all exists resistance to overturning poor, embedding body easily comes off, and causes the problems such as bacterium loss.
For the problems referred to above, the immobilized biological activated filler that it is good that we develop a kind of resistance to overturning is with a wide range of applications.
Summary of the invention
The object of the invention is to develop a kind of immobilization straight-tube shape biological active filling material based on mesh carrier, the reticulated structure design of this biological active filling material carrier, make embedding body be cross-linked to form overall rivet clasp structure by mesh and carrier, embedding body difficult drop-off, and embedding physical efficiency forms millimetre-sized membrane structure on reticulated structure, make the biological active filling material resistance to overturning that makes good, overall permeability is good, and mass transfer effect is higher.Mesh carrier is designed to straight-tube shape, and current can be accelerated the renewal of biological active filling material surface biological film to the tangent direction friction of biological active filling material, avoid varied bacteria growing, and hollow structure can make fluid pass, and can not cause microbial film to stop up.
In order to realize above-mentioned state, the present invention has adopted following technical scheme:
A kind of immobilization straight-tube shape biological active filling material based on mesh carrier, it is characterized in that: described straight-tube shape biological active filling material entirety is hollow straight-tube shape, the embedding body of the involved thalline of the meshed carrier of tool is cross-linked to form overall rivet clasp structure by mesh and carrier, and embedding body forms millimetre-sized membrane structure on reticulated structure.
Mesh carrier is taking polyethylene, polyvinyl chloride, polypropylene, ABS resin macromolecular material as main, add appropriate polyvinyl alcohol (PVA) water wetted material, by strand hot melt molding or sheet material hot pressing pore-forming mode, make the tubular structure carrier with netted barrel, tubular structure diameter is 0.5-3cm, height is 0.5-3cm, and the mesh shape of its barrel can be circle, rhombus, square, rectangle.The preferably string diameter 0.5-2mm of mesh.
The preparation method of above-mentioned a kind of straight-tube shape biological active filling material based on mesh carrier, is characterized in that, comprises the following steps:
(1) taking polyethylene, polyvinyl chloride, polypropylene, ABS resin macromolecular material as main, add appropriate polyvinyl alcohol (PVA) water wetted material, by modes such as strand hot melt molding or sheet material hot pressing pore-formings, make the straight-tube shape structure carrier of netted wall;
(2) embedding liquid polyvinyl alcohol solution and bacterium concentrated solution being mixed to form is uniformly coated on the outside surface of straight-tube shape structure carrier, according to polyvinyl alcohol-boric acid secondary crosslinking method, the straight-tube shape structure carrier that is coated with embedding liquid is put into after saturated boric acid solution 1-3h, regulate boric acid solution pH to 8-10, crosslinked 3-24h, taken out, cleaned remained on surface material, obtained biological active filling material.
The addition of polyvinyl alcohol (PVA) can suitably be adjusted as required, and this is prior art, and the quality percentage composition of preferably polyethylene alcohol (PVA) is 5-10%.
The straight-tube shape biological active filling material that the present invention is based on mesh carrier is applied to water treatment.
The beneficial effect of the immobilization straight-tube shape biological active filling material based on mesh carrier of the present invention is mainly reflected in:
1. cancellated design, makes embedding body form overall rivet clasp structure by mesh, and embedding body difficult drop-off makes the biological active filling material resistance to overturning that makes good, is conducive to the bacterial concentration that keeps higher;
2. mesh carrier is designed to straight-tube shape, current can impel biologic packing material to rotate along central shaft to tangential (barrel outside surface is tangential) friction of biological active filling material, and then the renewal of quickening biological active filling material surface biological film, avoid the growth of miscellaneous bacteria, hollow structure can make fluid pass, and can not cause tubular inner chamber microbial film to stop up;
3. experimental study shows, taking conventional filler as the carrier immobilized biological active filling material obtaining, and poor stability, in 2 days, embedded material almost all comes off, and the biological active filling material resistance to overturning obtaining with mesh carrier immobilization is good, the filler life-span is more than 3 years;
4. the biological active filling material that immobilization makes, global density can be close to water, be greater than water or be less than water, can be made into floated, precipitation or floatation type, can make the biological active filling material with certain function according to the demand of water treatment working condition, such as fixable type etc.;
5. mesh carrier is main raw by polyethylene, polyvinyl chloride, polypropylene, ABS resin, has added polyvinyl alcohol (PVA) water wetted material, has improved the wetting ability of carrier, has increased the intensity of carrier and embedding body bonding, has improved overall stability;
6. when biological active filling material makes embedding body can implementation structure thickness less due to the existence of mesh skeleton structure, do not lose again the variability of one-piece construction to water treatment running environment, less thickness can be realized higher mass-transfer efficiency and the higher utilization ratio of embedding body, cannot realize good carrier performance if make thickness like this with embedding body self material in water technology processing unit;
7. immobilized biological activated filler bag bacterium amount is large, can set up flora advantage, and processing efficiency is high;
8. the surface-area of biological active filling material is all useful area, has avoided the waste of material.
Brief description of the drawings
Fig. 1 is straight-tube shape biological active filling material schematic diagram;
Fig. 2 is I-I diagrammatic cross-section;
Fig. 3 is II-II diagrammatic cross-section.
1. twines in figure, 2. embedding body.
Embodiment
Below in conjunction with case study on implementation, the present invention is more specifically described, but embodiments of the present invention are not limited to this.
Example 1
1. the preparation of mesh carrier
Taking high molecular polymers such as polypropylene as main raw, add water wetted material polyvinyl alcohol (PVA) (preferably 5% content), making diameter is 0.5 ?1.5cm, and height is the straight-tube shape structure wire side carrier of 0.5 ?1.5cm, its mesh is shaped as square, and string diameter is 0.5mm.
2. the processing of nitrobacteria
Taking municipal wastewater treatment plant second pond excess sludge as bacterium source, by the nitrobacteria bacteria suspension centrifugal concentrating after screening enrichment culture, obtain 3.5 × 10
9the nitrobacteria concentrated solution of individual/mL.
3. obtaining of nitrobacteria embedding liquid
Weighing polyvinyl alcohol 500g, add 4000ml ultrapure water post-heating to 90 DEG C to dissolve 15min, after stirring, taking-up reheats to 90 DEG C of dissolving 5min, after taking-up, stir and be cooled to 30 DEG C, 4000ml polyvinyl alcohol solution and the 1000ml concentrated solution preparing are mixed, and add 100g calcium carbonate, after stirring, make 5000ml embedding liquid.
4. the combination of embedding liquid and mesh carrier
Embedding liquid is uniformly coated on to mesh carrier outside surface, guarantees that mesh carrier surface is all covered evenly by embedding liquid.
5. the preparation of nitrobacteria biological active filling material
The mesh carrier of smearing embedding liquid in step (4) is put into saturated boric acid solution, make it in submerged condition, leave standstill 3h, regulator solution pH to 9.0, standing 4h completes immobilization, cleans, and obtains nitrobacteria biological active filling material,
This biological active filling material proportion 0.95 ?between 1.10.
6. the application of biological active filling material activity
In the reactor of 100L, add embedded tubular biological active filling material, filling ratio is 30%, 25 ± 2 DEG C of water temperatures, HRT=4h, pH be 7.5 ?8.5, dissolved oxygen be 2.0 ?4.0mg/L, NH
4 +?N concentration is 160mg/L, and biological active filling material becomes suspension fluidized state, the on time nitrification effect of assaying reaction device under the effect of air lift.Water outlet detected result shows, reactor is cultivating 97.5% of the nitrification activity that returns to after 1 week before embedding, NH
4 +?N concentration below 3mg/L, clearance is more than 98.1%, coming off, do not appear in the continuous 3 years ' operation of biological active filling material, nitrification effect is stable.
Example 2
1. the preparation of mesh carrier
Taking high molecular polymers such as polyethylene as main raw, add water wetted material polyvinyl alcohol (PVA) (content 6%), making diameter is 1.0 ?2.0cm, and height is the straight-tube shape mesh carrier of 1.0 ?2.0cm, and its mesh is shaped as rhombus, string diameter 0.5mm.
2. the processing of nitrobacteria
Taking municipal wastewater treatment plant second pond excess sludge as bacterium source, by the nitrobacteria bacteria suspension centrifugal concentrating after screening enrichment culture, obtain 3.5 × 10
9the nitrobacteria concentrated solution of individual/mL.
3. obtaining of nitrobacteria embedding liquid
Weighing polyvinyl alcohol 5kg, add 40L ultrapure water post-heating to 90 DEG C to dissolve 15min, after stirring, taking-up reheats to 90 DEG C of dissolving 5min, after taking-up, stir and be cooled to 30 DEG C, 40L polyvinyl alcohol solution and the 10L nitrobacteria concentrated solution preparing are mixed, and add 1kg calcium carbonate, after stirring, make 50L embedding liquid.
4. the combination of embedding liquid and mesh carrier
Embedding liquid is uniformly coated on to straight-tube shape carrier surface, guarantees that mesh carrier surface is all covered evenly by embedding liquid.
5. the preparation of nitrobacteria biological active filling material
The mesh carrier of smearing embedding liquid in step (4) is put into saturated boric acid solution, make it in submerged condition, leave standstill 3h, regulator solution pH to 9.0, standing 4h completes immobilization, cleans, obtain nitrobacteria biological active filling material, this biological active filling material proportion 0.95 ?between 1.10.
6. the application of biological active filling material activity
To 1m
3laboratory in ripe A
2in the Aerobic Pond of O reactor, add embedded straight-tube shape biological active filling material, filling ratio is 30%, 20 ± 2 DEG C of water temperatures, HRT=2.5h, dissolved oxygen be 2.0 ?4.0mg/L, influent quality is city domestic sewage (NH
4 +?N concentration be 40 ?70mg/L), measure the nitrification ability of Aerobic Pond on time, detected result shows, reactor reaches steady state, water outlet NH cultivating after 1 week
4 +?N concentration be below 5mg/L, clearance, more than 90%, moves after 1 year still without embedding body obscission, Nitrification is stable.
Above-mentioned example shows, utilizing mesh carrier to carry out nitrobacteria immobilization, to prepare biological active filling material operating process simple, the overall rivet clasp structure that embedding body and carrier form by mesh, make that the biological active filling material resistance to overturning that makes is good, embedding body difficult drop-off, bacterial concentration is higher, can cultivate return to embedding after 1 week before nitrification activity 97.5%, and this biological active filling material good stability, long service life, has good application prospect.
Claims (4)
1. the straight-tube shape biological active filling material based on mesh carrier, it is characterized in that: described straight-tube shape biological active filling material entirety is hollow straight-tube shape, the embedding body of the involved thalline of the meshed carrier of tool is cross-linked to form overall rivet clasp structure by mesh and carrier, and embedding body forms millimetre-sized membrane structure on mesh carrier.
2. a kind of straight-tube shape biological active filling material based on mesh carrier of claim 1, it is characterized in that: mesh carrier is taking polyethylene, polyvinyl chloride, polypropylene or ABS resin macromolecular material as main, add appropriate polyvinyl alcohol (PVA) water wetted material, by strand hot melt molding or sheet material hot pressing pore-forming mode, make the tubular structure carrier with netted barrel, tubular structure diameter is 0.5-3cm, height is 0.5-3cm, the mesh of its barrel is shaped as circle, rhombus, square or rectangle, the string diameter 0.5-2mm of twine.
3. the method for a kind of straight-tube shape biological active filling material based on mesh carrier of preparation claim 1 or 2, is characterized in that, comprises the following steps:
(1) taking polyethylene, polyvinyl chloride, polypropylene, ABS resin macromolecular material as main, add appropriate polyvinyl alcohol (PVA) water wetted material, by strand hot melt molding or sheet material hot pressing pore-forming mode, make the straight-tube shape structure carrier of netted wall;
(2) embedding liquid bacterium liquid and polyvinyl alcohol solution being mixed to form is uniformly coated on the outside surface of straight-tube shape structure carrier, according to polyvinyl alcohol-boric acid secondary crosslinking method, the straight-tube shape carrier that is coated with embedding liquid is put into after saturated boric acid solution 1-3h, regulate boric acid solution pH to 8-10, crosslinked 3-24h, taken out, cleaned remained on surface material, obtained straight-tube shape biological active filling material.
4. any straight-tube shape biological active filling material based on mesh carrier of claim 1 or 2 is for water treatment.
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| CN201410137349.XA CN103951084B (en) | 2014-04-04 | 2014-04-04 | A kind of preparation of immobilization straight-tube shape biological active filling material and application based on mesh carrier |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105906035A (en) * | 2016-06-22 | 2016-08-31 | 北京工业大学 | Micropore biofilm carrier filler based on mesh structure |
| CN105923793A (en) * | 2016-06-22 | 2016-09-07 | 北京工业大学 | Micro-porous biological film based on mesh structure and used for fixedly adding carrier filler |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101746881A (en) * | 2009-12-11 | 2010-06-23 | 哈尔滨工业大学水资源国家工程研究中心有限公司 | Honeycomb network pipe bio-carrier |
| CN103193315A (en) * | 2013-04-12 | 2013-07-10 | 北京工业大学 | Method for preparing bioactive filler through immobilization of anaerobic ammonia oxidizing bacterium |
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2014
- 2014-04-04 CN CN201410137349.XA patent/CN103951084B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101746881A (en) * | 2009-12-11 | 2010-06-23 | 哈尔滨工业大学水资源国家工程研究中心有限公司 | Honeycomb network pipe bio-carrier |
| CN103193315A (en) * | 2013-04-12 | 2013-07-10 | 北京工业大学 | Method for preparing bioactive filler through immobilization of anaerobic ammonia oxidizing bacterium |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105906035A (en) * | 2016-06-22 | 2016-08-31 | 北京工业大学 | Micropore biofilm carrier filler based on mesh structure |
| CN105923793A (en) * | 2016-06-22 | 2016-09-07 | 北京工业大学 | Micro-porous biological film based on mesh structure and used for fixedly adding carrier filler |
| CN105906035B (en) * | 2016-06-22 | 2018-10-09 | 北京工业大学 | It is a kind of based on mesh-structured micropore biological membrane carrier filler |
| CN105923793B (en) * | 2016-06-22 | 2018-11-30 | 北京工业大学 | It is a kind of that fixed addition carrier filler is suitble to based on mesh-structured micropore biological film |
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