CN103951040A - Preparation and application of ammonia oxidizing bacteria immobilized bioactive filler based on polyurethane carrier - Google Patents
Preparation and application of ammonia oxidizing bacteria immobilized bioactive filler based on polyurethane carrier Download PDFInfo
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- CN103951040A CN103951040A CN201410137213.9A CN201410137213A CN103951040A CN 103951040 A CN103951040 A CN 103951040A CN 201410137213 A CN201410137213 A CN 201410137213A CN 103951040 A CN103951040 A CN 103951040A
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- oxidizing bacteria
- ammonia oxidizing
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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Abstract
The invention discloses preparation and an application of an ammonia oxidizing bacteria immobilized bioactive filler based on a polyurethane carrier, and belongs to the technical field of water treatment. The bioactive filler comprises two parts: an embedding body and the polyurethane carrier; a porous structure of the polyurethane foam carrier adsorbs a bacteria-containing embedding liquid, wherein the embedding liquid is prepared by mixing an ammonia oxidizing bacteria concentrated solution and a polyvinyl alcohol solution; a whole sheet of polyurethane foam is used as the embedding carrier, the embedding liquid is immersed to the interior of the polyurethane foam by a squeezing way, the embedding body is formed after boric acid secondary cross-linking and is combined with the polyurethane carrier to obtain a filler shape meeting requirements, and thus the ammonia oxidizing bacteria bioactive filler is obtained. The prepared ammonia oxidizing bacteria immobilized bioactive filler not only solves the problems that ammonia oxidizing bacteria are difficult in advantage establishment, easy to lose and the like, but also improves the processing ability of a reactor, shortens the start-up time, and has broad application prospects.
Description
Technical field
The invention belongs to water-treatment technology field, particularly a kind of preparation and application of the immobilized biological activated filler of ammonia oxidizing bacteria based on urethane carrier.
Background technology
Now, ammonia and nitrogen pollution problem is more and more serious, and ammonia nitrogen becomes 12 and reduces discharging new binding target, and promoting the ammonia and nitrogen pollution level of preventing and treating becomes the necessary behave that improves quality of water environment.At present, ammonia nitrogen waste water treatment method is divided into three major types, physico-chemical process, biochemical process and advanced oxidation processes.Biochemical process is due to technical maturity, and stable performance is acknowledged as a kind of economy, effective and the most rising method.Biochemical process comprises activated sludge process and biomembrance process.In traditional activated sludge process, by sludge reflux, keep amount of bacteria, just must increase quantity of reflux keeps high bacterial concentration to want to obtain high-level efficiency, will cause like this running cost to rise, obviously unactual.In addition, the generation cycle of nitrobacteria is long, and increment speed is slow, causes in reactor nitrobacteria concentration low, and processing efficiency is difficult to improve.Be subject to seasonal effect large.Summer temp raises, and biochemical speed is fast, and to winter, northern most of Sewage Plant all can face thread microbial sludge bulking problem, causes nitrifier to be run off, and then causes water outlet ammonia nitrogen to exceed standard.Therefore, can consider that applying biological embrane method makes up the shortcoming in activated sludge process, but biomembrance process has problems equally, for nitrobacteria, poor due to self film forming ability, natural membrane is chronic, and natural membrane non-selectivity, is difficult to form highdensity flora advantage.Various reasons shows, traditional biochemical process cannot reach efficient nitrification efficiency.
Nitration reaction is that ammonia oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) acting in conjunction complete, and AOB is oxidized to sub-nitrogen by ammonia nitrogen, and NOB further changes into nitre nitrogen by sub-nitrogen.Short distance nitration claims again nitrosification, and this technique can reduce 25% nitrated oxygen requirement, 40% denitrifying carbon source, 50% sludge yield and denitrification pool volume.
CN101941758 discloses " a kind of method of urethane using immobilized nitrifying bacteria treatment of Power nitrogenous effluent ", and its object is to provide a kind of method that improves the urethane using immobilized nitrifying bacteria treatment of Power nitrogenous effluent of sewage water denitrification processing efficiency.This technical scheme: the nitrobacteria that filters out high vigor from the aerobic activated sludge of denitrogenation Sewage treatment systems carries out amplification cultivation, it is standby that centrifugal concentrating is made nitrifier suspension, to the sodium alginate that adds 0.016g/L in the polyvinyl alcohol of 16wt%, dissolve after cooling and add equal-volume bacteria suspension, pour into afterwards in the spherical urethane carrier of diameter 0.5-1.0cm and adsorb, after 1-2 hour, add saturated boric acid to be cross-linked, make urethane using immobilized nitrifying bacteria and be added in fluidized-bed reactor and move.Its weak point is: by after the independent adsorption of immobilization of spherical urethane carrier of diameter 0.5-1.0cm, polyurethane surface can form the film of one deck densification, affects mass transfer, and processing efficiency is low, and spherical urethane is made difficulty.In addition, research shows, when urethane carrier diameter is greater than 0.5cm, carrier inside mass transfer is affected and forms dead band.Polyvinyl alcohol is 16wt%, and this massfraction concentration for urethane is carrier immobilized is bigger than normal, adopts the meeting of nature suction type because polyvinyl alcohol concentration is too high, and viscosity is large, causes urethane carrier inside cannot be adsorbed onto polyvinyl alcohol mixing liquid.In addition complete nitrification not only consumes energy greatly, and processing costs is high.
For the problems referred to above, we develop a kind of biological active filling material with efficient nitrification efficiency and are with a wide range of applications.
Summary of the invention
The object of the invention is to develop the immobilized biological activated filler of a kind of ammonia oxidizing bacteria based on urethane carrier, this biological active filling material adopts immobilized mode to make, can form efficient ammonia oxidizing bacteria flora advantage, and using polyurethane foam as carrier, polyurethane foam inside in moulding process has formed a lot of micropores, can be microorganism very good, relatively stable living environment is provided, be conducive to adhering to, breeding of microorganism.
The immobilized biological activated filler of ammonia oxidizing bacteria based on urethane carrier, is characterized in that: massive polyurethane carrier adsorbs embedding liquid by extruding and adsorbs the immobilized ammonia oxidizing bacteria that has.
In order to realize above-mentioned state, the present invention has adopted following technical scheme.
A preparation for the immobilized biological activated filler of ammonia oxidizing bacteria based on urethane carrier, is characterized in that: take the macromolecular materials such as polyurethane foam as embedding carrier; Take municipal wastewater treatment plant second pond excess sludge as bacterium source, and the ammonia oxidizing bacteria bacteria suspension centrifugal concentrating by after screening enrichment culture, obtains 10
8-10
9the ammonia oxidizing bacteria concentrated solution of individual/mL; According to polyvinyl alcohol-boric acid secondary crosslinking method, ammonia oxidizing bacteria concentrated solution and polyvinyl alcohol solution to be mixed, and add calcium carbonate and be prepared into embedding liquid, polyvinyl alcohol mass concentration is 30-50g/L, calcium carbonate mass concentration is 1g/L; Embedding liquid is poured in whole polyurethane foam block, repeatedly pushes polyurethane foam, make embedding liquid enter into polyurethane foam inside, put into after saturated boric acid solution 1-3h, regulate boric acid solution pH to 8-10, crosslinked 3-24h, taken out, cleaned remained on surface material, cutting; Be cut into satisfactory shape, its shape can be the square of length of side 3-5mm, the high rectangular parallelepiped that is respectively 3-5mm of length and width, and the right cylinder of diameter 3-5mm, high 3-5mm, obtains ammonia oxidizing bacteria biological active filling material.
The immobilized biological activated filler of the above-mentioned ammonia oxidizing bacteria based on urethane carrier is for water treatment.
The beneficial effect of the immobilized biological activated filler of ammonia oxidizing bacteria based on urethane carrier of the present invention is mainly reflected in:
1. the immobilized biological activated filler bag of ammonia oxidizing bacteria bacterium amount is large, can set up ammonia oxidizing bacteria flora advantage, realizes short distance nitration, and nitrosification efficiency is high;
2. urethane carrier can form a lot of internal cavities in moulding process, can be ammonia oxidizing bacteria very good, relatively stable living environment is provided, and is conducive to adhering to, breeding of ammonia oxidizing bacteria;
3. after crosslinked end, polyurethane foam is made to satisfactory shape, avoided crosslinked front cutting to cause each filling surface to form the film of one deck densification, be conducive to mass transfer, saved raw material;
4. biologic packing material shape can be the square of length of side 3-5mm, the high rectangular parallelepiped that is respectively 3-5mm of length and width, the right cylinder of diameter 3-5mm, high 3-5mm, experimental result shows, the biological active filling material of this range size can be in the inner dead band that forms of filler, and filler utilization ratio is high;
5. by fashion of extrusion, embedding immersion is entered to polyurethane foam inside, guaranteed that embedding liquid can immerse polyurethane foam inside completely;
6. this urethane carrier specific surface area is large, and good hydrophilic property, contributes to mass transfer, can improve water treatment effect;
7. ammonia oxidizing bacteria population advantage can be oxidized to ammonia control in sub-nitrogen, realizes short distance nitration and has not only saved the energy and also reduced processing costs;
8. the ammonia oxidizing bacteria biological active filling material that immobilization makes, whole proportion is between 0.98-1.05, and under gas stripping, fluidized state is good, does not exist corner to pile up, the problems such as current short circuit.
Accompanying drawing explanation
Fig. 1 is the immobilized biological activated filler schematic diagram of urethane carrier ammonia oxidizing bacteria;
Fig. 2 is the immobilized biological activated filler activation recovering of urethane carrier ammonia oxidizing bacteria and operating performance figure.
1. ammonia oxidizing bacteria embedding body, 2. urethane holes in figure
Embodiment
Below in conjunction with case study on implementation, the present invention is more specifically described, but embodiments of the present invention are not limited to this.
Embodiment 1
1. the processing of ammonia oxidizing bacteria
Take municipal wastewater treatment plant second pond excess sludge as bacterium source, and the ammonia oxidizing bacteria bacteria suspension centrifugal concentrating by after screening enrichment culture, obtains 3.5 * 10
9the ammonia oxidizing bacteria concentrated solution of individual/mL.
2. embedding liquid obtains
Weighing polyvinyl alcohol 200g, add 2500ml ultrapure water post-heating to 90 ℃ to dissolve 15min, after stirring, taking-up reheats to 90 ℃ of dissolving 5min, after taking-up, stir and be cooled to 30 ℃, 2500ml polyvinyl alcohol solution and the 2500ml concentrated solution preparing are mixed, and add 50g calcium carbonate, after stirring, make 5000ml embedding liquid.
3. the combination of embedding liquid and urethane carrier
Embedding liquid is poured in whole polyurethane foamed material (1m*1m*9mm), repeatedly pushed polyurethane foam, make embedding liquid can enter urethane inside.
4. the preparation of ammonia oxidizing bacteria biological active filling material
The urethane carrier that is stained with embedding liquid in step (3) is put into saturated boric acid solution, make it in submerged condition, standing 3h, regulator solution pH to 9.0, standing 4h completes immobilization, cleans, and is cut into the square that the length of side is 3-5mm, the urethane biological active filling material application limitation that the length of side is less than 3mm is large, and the inner mass transfer of urethane biological active filling material that the length of side is greater than 5mm can be affected and form dead band.After cutting, obtain ammonia oxidizing bacteria biological active filling material, this biological active filling material proportion is between 0.99-1.05.
5. the application of biological active filling material activity
In the reactor of 100L, add embedded ammonia oxidizing bacteria biological active filling material, filling ratio is 30%, 25 ± 2 ℃ of water temperatures, and HRT=5h, pH is 7.5-8.5, NH
4 +-N concentration is 160mg/L, and dissolved oxygen concentration is 1.0-1.5mg/L, and biological active filling material becomes suspension fluidized state, the on time nitrification effect of assaying reaction device under the effect of air lift.Water outlet detected result shows, reactor returns to 97.5% of nitrification activity before embedding, NH after cultivating 1 week
4 +-N concentration is below 5mg/L, and clearance is 96.8%, and sub-nitrogen accumulation rate is more than 90%, the continuous 3 years ' operation of biological active filling material, nitrosification effect stability.
Above-mentioned example shows, utilizing urethane carrier to carry out ammonia oxidizing bacteria immobilization, to prepare biological active filling material operating process simple, before can returning to embedding after cultivating 1 week 97.5% of nitrification activity, and this biological active filling material internal void is conducive to ammonia oxidizing bacteria and adheres to, breeds, nitrosification performance efficient stable, long service life, has good application prospect.
Claims (4)
1. a preparation for the immobilized biological activated filler of ammonia oxidizing bacteria based on urethane carrier, is characterized in that, comprises the following steps: take polyurethane foam macromolecular material as embedding carrier; Take municipal wastewater treatment plant second pond excess sludge as bacterium source, and the ammonia oxidizing bacteria bacteria suspension centrifugal concentrating by after screening enrichment culture, obtains 10
8-10
9the ammonia oxidizing bacteria concentrated solution of individual/mL; According to polyvinyl alcohol-boric acid secondary crosslinking method, ammonia oxidizing bacteria concentrated solution and polyvinyl alcohol solution to be mixed, and add calcium carbonate and be prepared into embedding liquid, polyvinyl alcohol mass concentration is 30-50g/L, calcium carbonate mass concentration is 1g/L; Embedding liquid is poured in whole polyurethane foam block, repeatedly pushes polyurethane foam, make embedding liquid enter into polyurethane foam inside, put into after saturated boric acid solution 1-3h, regulate boric acid solution pH to 8-10, crosslinked 3-24h, taken out, cleaned remained on surface material, cutting.
2. according to the method for claim 2, it is characterized in that: be cut into satisfactory shape, it is shaped as the square of length of side 3-5mm the high rectangular parallelepiped of 3-5mm or the right cylinder of diameter 3-5mm, high 3-5mm of being respectively of length and width.
3. the immobilized biological activated filler of the ammonia oxidizing bacteria based on urethane carrier preparing according to the method for claim 1 or 2.
4. the immobilized biological activated filler of the ammonia oxidizing bacteria based on urethane carrier of claim 1 is for water treatment.
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| CN201410137213.9A CN103951040A (en) | 2014-04-04 | 2014-04-04 | Preparation and application of ammonia oxidizing bacteria immobilized bioactive filler based on polyurethane carrier |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108641115A (en) * | 2018-05-14 | 2018-10-12 | 苏州健能新材料科技有限公司 | The preparation method of dual-component polyurethane foaming agent |
| CN111470625A (en) * | 2020-05-06 | 2020-07-31 | 北京工业大学 | Preparation and application of anaerobic hydrolysis acidification composite bacteria embedded bioactive filler |
| CN113023868A (en) * | 2021-02-27 | 2021-06-25 | 安徽久吾天虹环保科技有限公司 | Biological wax-embedded microorganism bioactive filler and preparation method and application thereof |
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| CN101941758A (en) * | 2010-08-19 | 2011-01-12 | 国核电力规划设计研究院 | Method for treating nitrogen-containing wastewater from power plants by nitrifying bacteria immobilized on polyurethane |
| CN101993867A (en) * | 2009-08-24 | 2011-03-30 | 浙江海正药业股份有限公司 | Immobilization method using chitosan as carrier |
| CN103194437A (en) * | 2013-04-12 | 2013-07-10 | 北京工业大学 | Method for finishing bacterium fixation by utilizing polyvinyl alcohol-borate secondary crosslinking |
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2014
- 2014-04-04 CN CN201410137213.9A patent/CN103951040A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101993867A (en) * | 2009-08-24 | 2011-03-30 | 浙江海正药业股份有限公司 | Immobilization method using chitosan as carrier |
| CN101941758A (en) * | 2010-08-19 | 2011-01-12 | 国核电力规划设计研究院 | Method for treating nitrogen-containing wastewater from power plants by nitrifying bacteria immobilized on polyurethane |
| CN103194437A (en) * | 2013-04-12 | 2013-07-10 | 北京工业大学 | Method for finishing bacterium fixation by utilizing polyvinyl alcohol-borate secondary crosslinking |
Non-Patent Citations (1)
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| 薛光: "《银的分析化学》", 31 October 1998, article " ", pages: 423-424 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108641115A (en) * | 2018-05-14 | 2018-10-12 | 苏州健能新材料科技有限公司 | The preparation method of dual-component polyurethane foaming agent |
| CN111470625A (en) * | 2020-05-06 | 2020-07-31 | 北京工业大学 | Preparation and application of anaerobic hydrolysis acidification composite bacteria embedded bioactive filler |
| CN111470625B (en) * | 2020-05-06 | 2022-03-29 | 北京工业大学 | Preparation and application of anaerobic hydrolysis acidification composite bacteria embedded bioactive filler |
| CN113023868A (en) * | 2021-02-27 | 2021-06-25 | 安徽久吾天虹环保科技有限公司 | Biological wax-embedded microorganism bioactive filler and preparation method and application thereof |
| CN113023868B (en) * | 2021-02-27 | 2023-09-01 | 安徽久吾天虹环保科技有限公司 | Biological wax embedded microorganism bioactive filler, preparation method and application |
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Application publication date: 20140730 |
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