CN103947904B

CN103947904B - A kind of preparation method for improving cow rumen flora glucose precursor thing

Info

Publication number: CN103947904B
Application number: CN201410204205.1A
Authority: CN
Inventors: 邵素英; 李建树
Original assignee: Ningxia Ruisheng Mingjie Intellectual Property Consulting Co ltd
Current assignee: Beijing Oriental Energy Biotechnology Co., Ltd.
Priority date: 2014-05-15
Filing date: 2014-05-15
Publication date: 2017-11-28
Anticipated expiration: 2034-05-15
Also published as: CN103947904A

Abstract

The invention discloses a kind of preparation method for improving cow rumen flora glucose precursor thing, belong to milk cow glucose precursor thing technical field.Using microorganism formulation and glucose precursor thing as primary raw material, science compounding Chinese herbal medicine extract, complex enzyme and other nutriments, the microorganism species and growth and the endogenous glucose needed for physiology needed for cow rumen are effectively supplemented and maintained.Through testing 16.81% can be improved per cow head daily yielding；Milk cow postpartum, estrus time shortened nearly 10 days first, and the conception rate of postpartum the first feelings phase milk cow improves 16.2%, the heat ratio increase by 16% of Postpartum Cows colony；The incidence of milk cow postpartum various diseases averagely reduces by 13.0 30.5%.The preparation method of glucose precursor thing of the present invention, the higher glycerine of viscosity and propane diols are uniformly mixed with other materials, technique is simple and convenient to operate, production efficiency is high, material good mixing effect, and large-scale production can be achieved.

Description

A kind of preparation method for improving cow rumen flora glucose precursor thing

Technical field

The present invention relates to milk cow glucose precursor thing, more particularly to a kind of improvement cow rumen flora glucose precursor thing Preparation method.

Background technology

Generally, most of sugar forms VFA (volatile fats during lumen fermentation in ruminant Acid), only a small amount of glucose is absorbed in alimentary canal.Ruminant blood glucose grow up than relatively low, but is not meant to neglect Depending on the meaning of glucose metabolism.Glucose is indispensable nutrients in all animal bodies as important trophism monose Matter, important effect is responsible in zooblast metabolism.Ketoacidosis easily occurs for glucose supplies deficiency, ox, and gestation sheep easily occurs Toxaemia, have a strong impact on growth and the health of animal.Therefore, people strengthen the research to glucose Nutrition and Metabolism.With same The extensive use of the plain technology in position, people have further to the metabolic rule of glucose under status in different nutritional status and physiological status Understand.At present, international research work is concentrated mainly on to glucose Nutrition and Metabolism rule in each tissue of ruminant The regulation and control of discussion and hormone to carbohydrate metabolism, and the domestic Nutrition and Metabolism research to ruminant glucose is still in Starting stage.

Energy needed for ruminant can not be provided individually by aliphatic acid, and some tissues and organ also especially need Portugal in vivo Grape sugar, such as：Central nervous system, adipose tissue, embryo and mammary gland, musculature etc., the generation of a. embryo growths and breast need Substantial amounts of glucose, ovine pregnancy later stage, embryo's glucose metabolism can account for the 40%-70% of ewe glucose total amount；In sheep breast Lactose content is equivalent to 90 times in blood.It was found that the glucose needed for the lactose of high yield cow synthesis daily is up to 2000g； The sheep galactopoiesis of bosom twin lamb(s) needs 200g glucose daily；Glucose consumed in the high ruminant synthesis lactose of milk yield accounts for The 60%-85% of overall glucose total amount.B. sheep nervous system needs glucose 17g daily, and it is total to account for animal overall glucose The 15%-20% of amount, and human brain will account for 70%-80%, other ruminants are between the mankind and sheep.C. it is known often to synthesize 1g body fat acid needs 0.65g glucose.Thus, glucose is extremely important to energy needed for ruminant.

The source of glucose is divided into Exogenous Glucose and Inner sources glucose, 1. Exogenous Glucoses in ruminant body：Feed Interior soluble sugar and starch is almost largely degraded through cud, and absorbed amount of glucose is very directly in alimentary canal Micro-, some starch of the cattle and sheep of a large amount of feed fine fodders can avoid lumen fermentation, be utilized into small intestine, the degraded of feed starch Degree is relevant with feed species and processing method.Under grazing condition, the glucose directly absorbed in sheep alimentary canal is several It can be ignored.It is estimated that the glucose that the sheep based on feed roughage only has 5-10g feed resources daily can be inhaled Receive；A large amount of glucose that body needs daily will lean on gluconeogenesis to synthesize in vivo.2. Inner sources glucose：One is in The sheep of maintenance state, if it can only obtain 5-10g glucose from feed daily, then acted on and closed by body glucose heteroplasia Into Inner sources glucose quantity up to 80-120g.It can be seen that ruminant is not that or need not need glucose less, but obtain The source of the glucose obtained is different from nonruminant.Inner sources glucose is the main source of required glucose in ruminant body, This is one of ruminant glucose metabolism and the main feature utilized.

As other ruminants, milk cow glucose metabolism is mainly shown as that liver sugar is different in lactation metabolism at initial stage change Raw increase and the glycoxidative declines of surrounding tissue, it is used to synthesize lactose to ensure that glucose can be directly entered breast tissue. The researchs such as Reynokls et al (2003) find, perinatal period and earlier lactating period milk cow internal organs portal system glucose it is net Flux is zero, or even slight negative occurs.9 days general outputs to 21 days postpartum internal organs glucose raise before the expected date of childbirth 267%, this almost all derives from the gluconeogenesis of liver.The main substrate of ruminant liver glycogen heteroplasia is sent out for cud The amino acid that propionate caused by ferment, lactate, protein metabolism or internal organ portal system caused by tricarboxylic acid cycle absorb with And the glycerine (Seal and Reynokls1993) that adipose tissue degraded is discharged.Perinatal period is from propionate, lactate and sweet The glucose of oily heteroplasia accounts for 50%-60%, 15%-20% and 2%-4% (Reynokls et of the net burst size of liver glucose al2003).By the glucose of amino acid heteroplasia at least up to the 20%-30% of glucose total amount, wherein by alanine heteroplasia Glucose was 2.3% at antenatal 9 days, and 5.5% is risen to postpartum 11d.With the above results and consistent, Overton etc. (1998) liver for reporting postpartum 1d is 2 times of antenatal 21d to doctor's ability of alanine.Although quantitatively see from amino The amino acid of sour pond release is unimportant with respect to for milk yield, but the above results show that amino acid can be used as a kind of adaptability bottom Thing is used for the synthesis of postpartum early stage glucose.

Precursor as gluconeogenesis has a many, most significant precursor be propionic acid, glycerine, amino acid and Lactic acid；It is other to also have valeric acid, nucleic acid, citric acid and other various organic acids, because several organic acids such as valeric acid contain in blood Amount is very low, and therefore, raw sugar effect is limited.

It facts have proved：All high yield cows are always threatened by ketoacidosis to some extent in earlier lactating period.It is particularly sub- Clinical ketoacidosis, due to not producing typical clinical symptoms, it is more difficult to identify, simply show cow feeding amount and decline, produce Milk amount reduces, spirit is depressed.These phenomenons but be generally interpreted as " new production ox feed intake is low, calving stress, mismanagement Etc. caused by composite factor ".And really the reason for is due to that to can not meet physiological status fast for the supply of glucose in Contents in Cows Great demand during speed change.In brief, milk cow lacks sugar.

Although occurring what symptom do not showed on the milk cow surface of subclinical ketosis, the milk production potentiality of usual milk cow by To very big influence.Recently, U.S. Hoard ' s Dairyman magazines are found by the range survey in the U.S., various new production Niu Changjian Disease the incidence of disease be successively from high to low：Ketoacidosis (clinical type and Subclinical), retained afterbirth, puerperal fever, displaced abomasums and son Endometrium is scorching.Investigation that Canadian Onatrio is saved is, it was also found that it is that ketoacidosis (face by clinical type and Asia that newly production ox, which goes wrong most, Bed-type), next to that mammitis (clinical type and Subclinical), followed by hoof disease, retained afterbirth, milk fever and displaced abomasums.By This is visible, and ketoacidosis (clinical type and Subclinical) is new production ox incidence of disease highest disease in milk cattle cultivating developed country.In State, because the enquiry based work of correlation is not yet popularized, but undeniably, ketoacidosis is to be hidden in the maximum milk production inside cattle farm Measure one of " killer ".

Science, rationally, effectively for milk cow provides metabolizable glucose can effectively improve the output of milk, reduction ketoacidosis and abomasum change The incidence of disease of position, effectively improve Lactation of Dairy Cow early stage dry matter intake and shorten calving interval and first rutting time.

It is exactly that raw sugared body, i.e. Portugal are added in feed that milk cow, which is solved, in the effective ways of earlier lactating period glucose supplies deficiency Grape sugar precursor thing.

Cow rumen dysbacteriosis is due to irrational raising dairy cattle way to manage, and the unreasonable of antibiotic medicine makes With causing milk cow alimentary canal, beneficial microbe is reduced or disappeared particularly in cud, and microbial ecological balance is destroyed and causes Cow rumen digests and assimilates hypodynamia, autonomic nerve disorder, further results in the gradual nutrient consumption sexual exhaustion of milk cow, most Cause milk cow superseded eventually or even dead, milk cow intractable apositia is claimed on veterinary clinic.As other ruminants, milk cow is most One of main feature is exactly that can utilize high-fiber feeding stuff extensively, and this feature has benefited from rumen microorganism, in milk cow forage Cellulose relies primarily on bacterium, infusorian and fungi in cud to decompose, in the decomposition digestion of cellulose, bacterium and fungi 80% effect is played, infusorian plays balance control action, and in the case where rumen microorganism acts synergistically, cellulose is decomposed paragraph by paragraph, It is final to produce volatile fatty acid, absorbed by milk cow.

A part of rumen microorganism is pernicious, and such as methane backeria, its main function is exactly synthesizing methane；Methane quilt It is considered to be only second to the important gas for causing global warming of carbon dioxide, the generation of methane is also to be fermented in cud in Contents in Cows The fodder energy of the main reason for energy loss, about 6-15% are depleted in the form of methane.

Therefore, beneficial microbe needed for reasonable supplement cow rumen can reduce the consumption of energy matter, promote endogenous Portugal The absorption and metabolism of grape sugar.

Chinese patent CN102247570B discloses a kind of Chinese medicine composition for treating ketosis of dairy cows, uses the Chinese traditional medicine composition Thing treats ketosis of dairy cows 58, and up to 86%, but without fundamentally solving, earlier lactating period glucose supplies are insufficient to ask cure rate Topic, cures the symptoms, not the disease, bad to primary ketoacidosis therapeutic effect.Chinese patent CN101822317B discloses a kind of preventing and treating milk cow The compound feed additive and preparation method of ketoacidosis.It is by Chinese traditional medicine angelica, the radix paeoniae rubrathe, Ligusticum wallichii, cultivated land, motherwort, costus root, party Ginseng, the Radix Astragali, hawthorn, extract and sodium propionate, cobaltous sulfate, copper sulphate, manganese sulfate, nicotinic acid, vitamine D3, phosphoric acid made of dried orange peel The compound preparation that calcium dihydrogen, citric acid collectively constitute, can air making-up and spleen enlivening, blood circulation promoting and enriching, increase glycogen alleviate acid poisoning, supplement lack Few trace element, mend sugar and replenish the calcium phosphorus, reach and ketoacidosis is fast and effectively prevented and treated, wherein Chinese medical extract use Water-boiling method extracts, and effective component extraction rate is low, and cost is high, while glucose precursor thing compounding is not complete, and resultant effect is bad, it is impossible to The microorganism species of cow rumen are maintained very well.Chinese patent CN101305774B discloses a kind of cow in perinatal period compound nutritional Replenishers, it is made up of the pre-mixing agent raw material of following composition by weight：Rumen bypass vitamin E：0.5-1 parts, rumen bypass beta carotene： 0.2-0.3 parts, rumen bypass nicotinic acid 5-6 parts, rumen bypass biotin：0.015-0.02 parts, rumen bypass choline：3-4 parts, lysine-zn 0.35-0.45 parts, glucose oxidase preparation：50-60 parts, brown sugar are added to 100 parts in right amount.Exempt from milk cow body is improved Epidemic disease power, protection hepatic and renal function, can prevent postpartum disease of cow；With reproductive performance is improved, shorten the function in calving interval；Tool There is promotion to be metabolized, the function such as increase milk yield, earlier lactating period milk cow endogenous glucose is insufficient to ask also without solving Topic, to the preventing of the diseases such as ketoacidosis, treat and the production performance of milk cow and other effects is not ideal.Chinese patent CN102499331B A kind of cow rumen regulation and control agent and preparation method thereof.The rumen regulation and control agent is by saccharomyces cerevisiae, candida tropicalis and meter Qu Mould is cultivated, the product then obtained after mixed fermentation, wherein, saccharomyces cerevisiae, candida tropicalis and meter Qu during fermentation The volume ratio of mould is 0.9~1.1: 1.8~2.2: 0.9~1.1.Advantage is promotion and safeguards that microbiota is put down in cud Weighing apparatus, stable ruminal pH value, alleviate milk cow acid poison caused by high fine fodder, reduce the concentration of ammonia in cud, promote its utilization to nitrogen, Improve its micro-ecological environment, but mainly by stimulating the breeding of cellulose-decomposing bacteria and lactic acid bacteria in cud to reach above-mentioned purpose, Without targetedly beneficial bacterium needed for supplement cud.

In summary, one kind is prepared to be effectively improved cow rumen microorganism species, significantly improve endogenous Portugal needed for milk cow The glucose precursor thing of grape sugar is necessary.

The content of the invention

Technical problem solved by the invention is to overcome the shortcomings of existing dairy cow nutrition material, for the feed feature of milk cow With digestion, absorption and metabolic characteristic, there is provided one kind can improve cow rumen digestion flora, the effectively growth of supplement milk cow and physiology institute The endogenous glucose needed is to prevent and treat milk cow intractable apositia and due to a lack of various diseases caused by glucose The preparation method of glucose precursor thing.

In order to achieve the above object, the present invention uses following technical scheme：

A kind of preparation method for improving cow rumen flora glucose precursor thing, comprises the following steps：

The glucose precursor thing is prepared by the raw material of following parts by weight：

Microorganism formulation 45-55 parts, propane diols 22-28 parts, glycerine 22-28 parts, calcium propionate 22-28 parts, alumino-silicate 22- 28 parts, Chinese herbal medicine extract 18-22 parts, niacinamide 12-18 parts, nicotinic acid 12-18 parts, sodium lactate 10-15 parts, sodium chloride 10-15 Part, complex enzyme 8-12 parts, citric acid 5-10 parts, rumen glucose 5-10 parts, rumen-bypass amino acid 3-8 parts, rumen bypass choline 5-8 parts, B B-complex 3-5 parts, composite mineral matter 2-4 parts.

The microorganism formulation is to occupy cud clostridium (Clostridium ruminocolum) and aspergillus niger (Aspergillus niger) blended fermentation, low-temperature negative-pressure vacuum concentration, fluidized bed drying, low-temperature grinding and be made；

Described cud clostridium (Clostridium ruminocolum) bacterial strain that occupies discloses for Chinese patent CN101148651B One plant occupy cud clostridium (Clostridium ruminocolum) H1, the clostridium has degraded cellulose and zytase, wood The activity of sugar and Ester；It is preserved within 6th in Chinese microorganism strain administration committee common micro-organisms in August in 2007 The heart, preservation address：City of BeiJing, China Chaoyang District great Tun roads first 3, deposit number：CGMCC NO.2125；

Aspergillus niger (Aspergillus niger) bacterial strain is specially the bacterial strain aspergillus niger for producing high activity cellulase (Aspergillus niger)Li-2013-03.The bacterial strain is preserved in Chinese microorganism strain preservation on July 15th, 2013 (abbreviation CGMCC, address are administration committee's common micro-organisms center：City of BeiJing, China Chaoyang District North Star West Road 1 institute 3, in Institute of microbiology of the academy of sciences of state, postcode 100101), preserving number is CGMCC NO.7927.

The preparation method of the microorganism formulation comprises the following steps：

(1) aspergillus niger CGMCC NO.7927, occupy cud clostridium CGMCC NO.2125 actication of culture

Intact aspergillus niger CGMCC NO.7927 slant strains are inoculated in slant medium, 28-33 DEG C of culture 36-48h carries out actication of culture, so activation 2-4 times；

The intact slant strains for occupying cud clostridium CGMCC NO.2125 are inoculated in slant medium, 35-41 DEG C Cultivate 24-36h and carry out actication of culture, so activation 2-3 times；

The aspergillus niger CGMCC NO.7927 slant mediums form：Potato (liquor) 200g, glucose 20g, fine jade Fat 20g, Chinese herbal medicine extract 5-20g, distilled water l000mL, 5.8,121 DEG C of sterilizing 20min of pH value；

It is described occupy cud clostridium CGMCC NO.2125 slant mediums composition be：Beef extract 3g, sodium chloride 5g, peptone 10g, agar 15g, Chinese herbal medicine extract 5-10g, distilled water l000mL, 6.8,121 DEG C of sterilizing 20min of pH value；

(2) aspergillus niger CGMCC NO.7927 liquid seeds expand culture

1. first order seed culture：Aspergillus niger CGMCC NO.7927 slant strains after step (1) is activated are with sterile washing Lower spore, access in 500 milliliters of shaking flasks, 100 milliliters of liquid seed culture medium loading amount, 28-33 DEG C, 80-120rpm shaking table cultures 36-48h；

2. secondary seed culture：First order seed is accessed in 500 milliliters of secondary seed shaking flasks according to 10% inoculum concentration, training The condition of supporting is identical with first order seed；

3. three-level seed culture：Secondary seed is accessed in 5000 milliliters of three-level seed flasks with 8% inoculum concentration, liquid training Support 1000 milliliters of base loading amount, 28-33 DEG C, 80-120rpm shaking table cultures 36-48h；

4. first class seed pot culture：Three-level seed is accessed into first class seed pot of the total measurement (volume) as 150L, hair using 8% inoculum concentration Ferment culture medium loading amount 100L, control ph 5-6,28-30 DEG C of cultivation temperature, mixing speed 200-400rpm, ventilation (V/V) 1:0.8-1.2, incubation time 36-48h, dissolved oxygen 10-20%；

The aspergillus niger CGMCC NO.7927 one-levels, two level, three-level seed culture medium composition are：Potato (liquor) 200g, glucose 20g, Chinese herbal medicine extract 15-20g, trehalose 10-30g, distilled water l000mL, 5.5,121 DEG C of sterilizings of pH value 20min；

The aspergillus niger CGMCC NO.7927 first class seed pots culture medium forms：Corn flour 50-60g, bean powder 15- 25g, wheat bran 10-15g, Chinese herbal medicine extract 15-20g, trehalose 10-30g, ammonium sulfate 1-3g, dipotassium hydrogen phosphate 1-2g, phosphoric acid Potassium dihydrogen 1-2g, pure water l000mL, pH value 5-7,121 DEG C of sterilizing 20min；

The aspergillus niger CGMCC NO.7927 first class seed pot zymotic fluids cell concentration is 7.0x10⁹-8.0x10⁹Individual/ml；

(3) cud clostridium CGMCC NO.2125 liquid seeds are occupied and expand culture

1. first order seed culture：The access of cud clostridium CGMCC NO.2125 slant strains is occupied after step (1) is activated In 500 milliliters of shaking flasks, 100 milliliters of culture medium loading amount, 180 revs/min of rotary shaker, 35-41 DEG C of cultivation temperature, incubation time 10-15h；

3. three-level seed culture：Secondary seed is accessed in 5000 milliliters of three-level seed flasks with 10% inoculum concentration, culture 1000 milliliters of base loading amount, 100 revs/min of rotary shaker, 35-41 DEG C of cultivation temperature, incubation time 10-15h；

4. first class seed pot culture：Three-level seed is accessed into first class seed pot of the total measurement (volume) as 150L using 10% inoculum concentration, Fermentation medium loading amount 100L, 37-39 DEG C of cultivation temperature, mixing speed 200-400rpm, ventilation (V/V) 1:1-2, tank pressure 0.05Mpa, incubation time 10-20h；

It is described occupy cud clostridium CGMCC NO.2125 one-levels, two level, three-level seed culture medium weight composition be：

Dusty yeast 0.3-0.5%, glucose 1-1.5%, peptone 0.3-0.5%, beef extract 0.5-0.8%, phosphoric acid hydrogen Dipotassium 0.8-1.5%, Chinese herbal medicine extract 1.5-2%, trehalose 1-3%, insufficient section pure water are supplied, pH value 6.8,121- 123 DEG C of sterilizing 30-40min.

It is described occupy cud clostridium CGMCC NO.2125 seed tank culture bases weight composition be：

Maltodextrin 5-15%, dusty yeast 0.4-0.8%, Chinese herbal medicine extract 1.5-2%, trehalose 1-3%, peptone 0.1-0.5%, corn steep liquor 0.1-0.5%, dipotassium hydrogen phosphate 0.8-1.5%, magnesium sulfate 0.05-0.1%, insufficient section pure water Supply, pH value 6.8,121-123 DEG C of sterilizing 30-40min.

The cud clostridium CGMCC NO.2125 first class seed pot zymotic fluids cell concentrations that occupy are 7.0x10⁹-8.0x10⁹ Individual/ml；

(4) aspergillus niger CGMCC NO.7927, occupy cud clostridium CGMCC NO.2125 first class seed pots liquid seeds mixing

The aspergillus niger CGMCC NO.7927 seeding tanks liquid seeds that step (2) obtains are occupied into cud with what step (3) obtained Clostridium CGMCC NO.2125 seeding tanks liquid seeds uniformly mix by volume；

The volume ratio 1：0.5-1；

The mixing liquid seed cell concentration is：7.0x10⁹-8.0x10⁹Individual/ml；

(5) secondary seed tank alternate

Mixed uniformly first class seed pot liquid seeds in step (4) are accessed into total measurement (volume) as 300L's using 10% inoculum concentration Secondary seed tank, alternate culture medium loading amount 240L, secondary seed tank fermentation tank, 33-39 DEG C of cultivation temperature, mixing speed 200- 700r/m, ventilation (V/V) 1:1-3, incubation time 15-24h；

The alternate culture medium forms：Maltodextrin 50-150g, corn flour 50-60g, bean powder 15-25g, wheat bran 10- 15g, Chinese herbal medicine extract 15-20g, trehalose 10-30g, dusty yeast 4-8g, corn steep liquor 1-5g, ammonium sulfate 1-3g, phosphoric acid hydrogen two Potassium 1-2g, potassium dihydrogen phosphate 1-2g, pure water l000mL, pH value 5-7,121 DEG C of sterilizing 20min；

The secondary seed tank zymotic fluid cell concentration is 7.0x10⁹-8.0x10⁹Individual/ml；

(6) strain mixed fermentation

Secondary seed tank alternate liquid seeds in step (5) are accessed into fermentation tank, cultivation temperature 28-30 with 6% inoculum concentration DEG C, mixing speed 200-700r/m, ventilation (V/V) 1:1-3, incubation time 15-20h；Then delayed with 1-2 DEG C/h rate of temperature fall Slowly 10-15 DEG C is cooled to, incubated 10-12h；Continue now, to walk to 2-5 DEG C with 1-2 DEG C/h rate of temperature fall slow cooling Suddenly secondary seed tank alternate liquid seeds add access fermentation tank, incubated 6-8h with 4% inoculum concentration in (5)；Finally with 1-2 DEG C/h heating rates are to slowly warm up to 10-15 DEG C, incubated 10-12h；Continuation is to slowly warm up to 1-2 DEG C/h heating rates 37-39 DEG C, incubated 15-20h；

The fermentation medium forms：Maltodextrin 50-150g, corn flour 50-60g, bean powder 15-25g, wheat bran 10- 15g, Chinese herbal medicine extract 30-50g, trehalose 30-40g, dusty yeast 4-8g, corn steep liquor 1-5g, ammonium sulfate 1-3g, phosphoric acid hydrogen two Potassium 1-2g, potassium dihydrogen phosphate 1-2g, pure water l000mL, pH value 5-7,121 DEG C of sterilizing 20min；

The ferment tank liquid cell concentration is 7.0x10⁹-8.0x10⁹Individual/ml；

(7) zymotic fluid is concentrated in vacuo to the 45% of original volume through low-temperature negative-pressure, obtains bacterium concentrate, and concentrate is pressed with carrier Mass ratio is 0.5:1 uniformly mixing, in 45 DEG C of fluidized bed dryings, 40 DEG C of low-temperature grindings, grinding particle size 0.5-1.5mm, is produced micro- Biological agent；

The vehicle weight number forms：CaCO₃20-23 parts, dextrin 10-12 parts.

The microorganism formulation viable count content is 1.5x10¹⁰-3x10¹⁰Individual/g.

The glycerine is food grade glycerin, purity 99.5%；

The alumino-silicate is alumino-silicate or alumino-silicate derivatives with the strong suction-operated of selectivity, with Chinese herbal medicine Extract science compounds, and a variety of toxin such as energy adsorption of aflatoxin, vomitoxin, zearalenone, T-2 toxin, also has Strong kidney of strengthen immunity, protect liver etc. acts on, and will not the nutrition such as amino acid, vitamin, trace element in adsorption feed；

The Chinese herbal medicine extract is prepared by the raw material of following parts by weight：Radix Angelicae Sinensis 55-65 parts, Ligusticum wallichii 55-65 parts, fructus amomi 50-60 parts, radix paeoniae rubrathe 50-60 parts, cultivated land 50-60 parts, Divine Comedy 45-55 parts, malt 45-55 parts, motherwort 45-55 parts, costus root 40-45 parts, Radix Codonopsis 40-45 parts, rhizoma atractylodis 40-45 parts, Radix Astragali 40-45 parts, honeysuckle 40-45 parts, cordate houttuynia 35-45 parts, Poria cocos 35-45 parts, jujube kernel 35-45 parts, fushen's 35-45 parts, polygala 30-40 parts, fry fennel 30-40 parts, bighead atractylodes rhizome 30-40 parts, bark of official magnolia 30- 40 parts, Fructus Aurantii 25-35 parts, radix glycyrrhizae 25-35 parts, hawthorn 25-35 parts, dried orange peel 25-35 parts.

The preparation method of the Chinese herbal medicine extract is：Said herbal medicine is crushed to particle diameter as less than 2 millimeters respectively, so After uniformly being mixed in container and adding the water of 3-6 times of weight, -90 DEG C of holding 2-4h of control temperature 70 C, 45- is then cooled to 60 DEG C, the mixing enzyme preparation for adding mixed material gross weight 5-10% is digested, and is 5.5-6.8 with newborn acid for adjusting pH value, enzyme 2-4h is solved, finally adds the mixture of 0.5-3 times of w ethanol of mixed material and propyl alcohol, control temperature to 60 DEG C of -78 DEG C of holdings 3-4h, filtering；It is more than 20% that filtrate decompression, which is concentrated into solid content, during then freeze-drying, 40 DEG C of low-temperature grindings produce Herb extracts, grinding particle size 0.5-2mm；

The parts by weight of the mixed enzyme form：Endo-beta-glucanase 10-20 parts, outer 1,4 beta-glucanase 10-20 parts, β- Glucuroide 10-15 parts, zytase 15-20 parts, pentosanase 15-20 parts, Pullulanase 20-30 parts, beta amylase 10- 15 parts, neutral proteinase 10-15 parts, acid protease 10-15 parts, superoxide dismutase 5-10 parts, glucose oxidase 5- 10 parts, acid phosphatase 5-10 parts；

The mass ratio of ethanol and the propyl alcohol mixing is 1:1-2, concentration of alcohol >=75%；

The sodium lactate is 60% food grade lactic acid sodium；

The complex enzyme is uniformly mixed by the solid polypeptide formulation of following parts by weight：Neutral proteinase 22-28 parts, acid Property protease 22-28 parts, alkalescent xylanase 22-28 parts, acidic xylanase 22-28 parts, cellulase 22-28 parts, β-Portugal Dextranase 15-20 parts, pectase 15-20 parts, amylase 15-20 parts, seminase 10-15 parts, phytase 10-15 parts.

The citric acid is solid citric acid；

The per kilogram B B-complex includes dehydroretinol 800mg, orotic acid 000mg, Catergen 000mg, core Flavine 1600mg, pantothenic acid 1900mg, vitamin B6 1800mg, vitamin B12 1200mg, inositol 5000mg, vitamin E 600mg, Vitamine D3 5500mg, Vitamin K3 4000mg；Its surplus is carrier, and the carrier is rice bran.

The per kilogram composite mineral matter includes calcium 6000mg, phosphorus 4200mg, sodium 5100mg, copper 800mg, iron 1800mg, Potassium 3800mg, manganese 1400mg, cobalt 1600mg, iodine 5000mg, magnesium 1450mg；Its surplus is carrier, and the carrier is rice bran.

Preparation method：

1) each raw material is accurately weighed according to above-mentioned formula rate, propane diols and glycerine is separately added into its quality 0.5- first 1 times of sterilized water is diluted, and then mixes dilution, while heating water bath is to 30-50 DEG C, while being stirred to obtain mixture A, heat preservation for standby use；

2) calcium propionate, alumino-silicate, sodium lactate, sodium chloride, citric acid are uniformly mixed into obtain mixture B；

3) it is Chinese herbal medicine extract, rumen glucose, rumen-bypass amino acid, rumen bypass choline, composite mineral matter is uniform Mix to obtain mixture C；

4) microorganism formulation, niacinamide, nicotinic acid, complex enzyme, B B-complex are uniformly mixed into obtain mixture D；

5) mixture B is added into LDH-1 type coulter type mixers, starts mixer, be subsequently added into mixture A and uniformly mix 10-20min；Then mixture C is added, uniformly mixes 5-10min；Mixture D is eventually adding, uniformly mixes 3-5min, will most The sealing of whole mixture, pack and produce the glucose precursor thing that can improve cow rumen flora.

Application method：1. being added in fine fodder, coarse fodder or premix, it is well mixed；

It is uniformly oral after mixing or be sprayed on TRM 2. this product is added into 10-20 times of running water；

Usage amount：New production ox：100-120g/ heads, day；

Cow in milk：80-100g/ heads, day；

Late drying period ox：100-120g/ heads, day；

Beef cattle：20-25kg/ ton daily rations；

Service stage：

1. improve production performance：Antenatal 20 days to 30 days postpartum；

2. prophylactic treatment ketoacidosis：Postpartum prevention and health care：The 1st day to the 7th day postpartum；

Postpartum therapy ketoacidosis：Postpartum determines blood ketone content, continuous to fill if content reaches more than 1.2mmol/L and gavaged Take 5 days；

The present invention occupy cud clostridium bacterial strain CGMCC NO.2125 can degraded cellulose and xylan, xylose and esters, should Bacterium contains the compound enzyme system of degraded cellulose, including exocellulase, endo cellulase, zytase, mannonase Esterase, pectase, enzyme activity be respectively 4.1 ± 1.19U/mg, 118.7 ± 4.5U/mg, 167.36 ± 22.9U/mg, 45.9 ± 3.52U/mg, 4 ± 0.5U/mg, 35.3 ± 1.77U/mg, most suitable 35-41 DEG C of the cultivation temperature of the bacterium, optimum pH 6.5-7.5.

Aspergillus niger strain CGMCC No.7927 of the present invention have following microbial characteristic：1st, morphological feature：Biology Form includes several parts such as conidium, born of the same parents' stalk, top capsule, production born of the same parents' structure.Conidial head is spherical to Radiation, diameter 150- 450 μm, conidiophore betides matrix.Born of the same parents' stem 1000-3000 (length) × 12-20 (diameter) μm, yellow or yellowish-brown, Wall is smooth；Top capsule is spherical or almost spherical, and 45-75 μm of diameter, surface is comprehensively fertile；It is double-deck to produce born of the same parents' structure, metulae 10-20 is (long Degree) × 4.5-7.0 (diameter) μm, bottle stalk 6-10 (length) × 2.5-3.5 (diameter) μm, conidium is spherical or subsphaeroidal, directly 3-4.5 μm of footpath, brown, wall are coarse.2nd, feature is learned in culture：Bacterial strain grows rapidly on wort agar culture medium, 28 DEG C of 4 days spores Son can be paved with inclined-plane；Quality velvet shape or slightly with cotton-shaped；Conidium structure is a large amount of, brown-black, no diffusate；Bacterium colony reverse side omits Band yellow.3rd, physiological and biochemical property：Aspergillus niger strain CGMCC No.7927 can be in maize straw, straw, wood chip, potato, jade Grown in the carbon sources such as ground rice, soluble starch, molasses, optimal pH scope 5-6,28-33 DEG C of optimum growth temperature scope, most suitable production 28-30 DEG C of enzyme temperature range.

Aspergillus niger strain CGMCC No.7927 of the present invention triage techniques route is：Starting strain → inclined-plane culture → spore Preparation → mutagenic treatment of sub- suspension → flat board separation → primary dcreening operation → secondary screening → genetic stability measure → expansion experiment (Fermented It can determine).

By mutagenesis screening scheme, mutant strain step-sizing is eliminated, finally the fermented performance test of strain excellent screened, A plant height producing enzyme performance bacterial strain black-koji mould Aspergillus niger Li-2013-03 are obtained, cellulose after fermenting 96 hours Circumscribed 1,4 beta-glucanase, Endo-β-glucanase, beta-glucosidase and the filter paper enzyme activity of enzyme respectively reach 620U/mL, 1289U/mL, 456U/mL and 732U/mL.

Beneficial effect：

1. glucose precursor thing prepared by the present invention is directed to the feed feature and digestion of milk cow, absorption and metabolic characteristic, with Microorganism formulation and glucose precursor thing are primary raw material, and science is compounded needed for Chinese herbal medicine extract, complex enzyme and other milk cows Nutriment, not only supplement and maintain the microorganism species needed for cow rumen, ensure that the normal function of cud, promote and Improve the digestibility and absorptivity of nutriment；And provide production, growth and prevention for milk cow, treat the most rich of disease Rich, most comprehensive nutriment, the endogenous glucose needed for particularly effectively supplemented with milk cow growth and physiology, is effectively maintained Milk cow human body energy balances.

2. glucose precursor thing prepared by the present invention can effectively improve the output of milk, can be improved per cow head daily yielding 16.81%；Effectively shorten nearly 10 days of the time of milk cow postpartum heat, improve the conception rate of postpartum the first feelings phase milk cow 16.2%, increase the heat ratio 16% of Postpartum Cows colony, effectively improve the reproductive performance of milk cow；Milk cow production can be significantly reduced The generation of the disease such as intractable apositia, ketoacidosis, mammitis, endometritis, cud displacement, puerperal fever and retention of afterbirth afterwards Rate, averagely reduces 13.0-30.5%, and t examines significant difference (P ＜ 0.05).

3. the Chinese herbal medicine extract of the present invention is extracted having in kind of herbal medicine to greatest extent using enzymolysis and alcohol precipitation extraction Composition is imitated, raw material availability is improved, reduces production cost.Also, the Chinese herbal medicine extract of the present invention is applied in micro- life In the preparation method of thing preparation, resistance of the rumen microorganism to Chinese herbal medicine is improved, is established for the normal operation of later stage digestive system Determined solid foundation, at the same overcome in the prior art in response to Chinese herbal medicine and to cud beneficial microbe suppress or destroy Defect；The preparation method of microorganism formulation of the present invention has resisting stress and rejuvenation effect to microorganism, micro- in microorganism formulation Biology has been provided with the resistance to the action of a drug to Chinese herbal medicine effective ingredientses, and the later stage Chinese herbal medicine effective ingredientses of feeding will not suppress and influence to add The normal growth of microorganism and breeding.

4. the alumino-silicate of the present invention has selective absorption function and powerful suction-operated, only adsorb in milk cow feed Mycotoxin caused by because of mould, does not adsorb microorganism and nutriment, improves the security of cow producing milk and effectively pre- Various diseases caused by mould proof verticillium toxin.

5. the preparation method of the present invention, the higher glycerine of viscosity and propane diols are uniformly mixed with other materials, technique letter It is single, easy to operate, production efficiency is high, material good mixing effect, can be achieved large-scale production.

Embodiment

The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention It is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, it is not intended to limit the present invention Scope, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, without departing substantially from this The various changes carried out on the premise of invention spirit and scope to the material component in these embodiments and dosage or change Belong to protection scope of the present invention.

The preparation of the microorganism formulation of embodiment 1

Intact aspergillus niger CGMCC NO.7927 slant strains are inoculated in slant medium, 30 DEG C of culture 24h Carry out actication of culture, so activation 3 times；

The intact slant strains for occupying cud clostridium CGMCC NO.2125 are inoculated in slant medium, 39 DEG C of trainings Support 24-36h and carry out actication of culture, so activation 2 times；

The aspergillus niger CGMCC NO.7927 slant mediums form：Potato (liquor) 200g, glucose 20g, fine jade Fat 20g, Chinese herbal medicine extract 10g, distilled water l000mL, 5.8,121 DEG C of sterilizing 20min of pH value；

It is described occupy cud clostridium CGMCC NO.2125 slant mediums composition be：Beef extract 3g, sodium chloride 5g, peptone 10g, agar 15g, Chinese herbal medicine extract 8g, distilled water l000mL, 6.8,121 DEG C of sterilizing 20min of pH value；

(2) aspergillus niger CGMCC NO.7927 liquid seeds expand culture

1. first order seed culture：Aspergillus niger CGMCC NO.7927 slant strains after step (1) is activated are with sterile washing Lower spore, access in 500 milliliters of shaking flasks, 100 milliliters of liquid seed culture medium loading amount, 30 DEG C, 100rpm shaking table cultures 24h；

3. three-level seed culture：Secondary seed is accessed in 5000 milliliters of three-level seed flasks with 8% inoculum concentration, liquid training Support 1000 milliliters of base loading amount, 30 DEG C, 100rpm shaking table cultures 24h；

4. first class seed pot culture：Three-level seed is accessed into first class seed pot of the total measurement (volume) as 150L, hair using 8% inoculum concentration Ferment culture medium loading amount 100L, control ph 5.5,30 DEG C, mixing speed 300rpm of cultivation temperature, ventilation (V/V) 1:1.2, Incubation time 42h, dissolved oxygen 15%；

The aspergillus niger CGMCC NO.7927 one-levels, two level, three-level seed culture medium composition are：Potato (liquor) 200g, glucose 20g, Chinese herbal medicine extract 15g, trehalose 20g, distilled water l000mL, 5.5,121 DEG C of sterilizing 20min of pH value；

The aspergillus niger CGMCC NO.7927 first class seed pots culture medium forms：Corn flour 55g, bean powder 20g, wheat bran 12g, Chinese herbal medicine extract 18g, trehalose 20g, ammonium sulfate 2g, dipotassium hydrogen phosphate 2g, potassium dihydrogen phosphate 2g, pure water L000mL, 5.5,121 DEG C of sterilizing 20min of pH value；

The aspergillus niger CGMCC NO.7927 first class seed pot zymotic fluids cell concentration is 8.0x10⁹Individual/ml；

(3) cud clostridium CGMCC NO.2125 liquid seeds are occupied and expand culture

1. first order seed culture：The access of cud clostridium CGMCC NO.2125 slant strains is occupied after step (1) is activated In 500 milliliters of shaking flasks, 100 milliliters of culture medium loading amount, 180 revs/min of rotary shaker, 39 DEG C of cultivation temperature, incubation time 12h；

3. three-level seed culture：Secondary seed is accessed in 5000 milliliters of three-level seed flasks with 10% inoculum concentration, culture 1000 milliliters of base loading amount, 100 revs/min of rotary shaker, 39 DEG C of cultivation temperature, incubation time 12h；

4. first class seed pot culture：Three-level seed is accessed into first class seed pot of the total measurement (volume) as 150L using 10% inoculum concentration, Fermentation medium loading amount 100L, 39 DEG C, mixing speed 300rpm of cultivation temperature, ventilation (V/V) 1:1, tank pressure 0.05Mpa, training Support time 15h；

Dusty yeast 0.4%, glucose 1.5%, peptone 0.4%, beef extract 0.6%, dipotassium hydrogen phosphate 1.2%, medium-height grass Medicament extract 1.5%, trehalose 2%, insufficient section pure water are supplied, 6.8,121 DEG C of sterilizing 30min of pH value.

Maltodextrin 10%, dusty yeast 0.6%, Chinese herbal medicine extract 1.6%, trehalose 2%, peptone 0.3%, corn Slurry 0.3%, dipotassium hydrogen phosphate 1.2%, magnesium sulfate 0.08%, insufficient section pure water are supplied, 6.8,121 DEG C of sterilizings of pH value 30min。

The cud clostridium CGMCC NO.2125 first class seed pot zymotic fluids cell concentrations that occupy are 8.0x10⁹Individual/ml；

The volume ratio 1：1；

The mixing liquid seed cell concentration is：8.0x10⁹Individual/ml；

(5) secondary seed tank alternate

Mixed uniformly first class seed pot liquid seeds in step (4) are accessed into total measurement (volume) as 300L's using 10% inoculum concentration Secondary seed tank, alternate culture medium loading amount 240L, secondary seed tank fermentation tank, 36 DEG C, mixing speed 500r/m of cultivation temperature, lead to Air quantity (V/V) 1:1, incubation time 20h；

The alternate culture medium forms：Maltodextrin 100g, corn flour 55g, bean powder 20g, wheat bran 12g, Chinese herbal medicine carry Take thing 18g, trehalose 20g, dusty yeast 6g, corn steep liquor 3g, ammonium sulfate 2g, dipotassium hydrogen phosphate 2g, potassium dihydrogen phosphate 2g, pure water L000mL, 6,121 DEG C of sterilizing 20min of pH value；

The secondary seed tank zymotic fluid cell concentration is 8.0x10⁹Individual/ml；

(6) strain mixed fermentation

By secondary seed tank alternate liquid seeds in step (5) with 6% inoculum concentration access fermentation tank, 30 DEG C of cultivation temperature, Mixing speed 500r/m, ventilation (V/V) 1:1, incubation time 18h；Then with 2 DEG C/h rate of temperature fall slow cooling to 12 DEG C, Incubated 10h；Continue with 2 DEG C/h rate of temperature fall slow cooling to 4 DEG C, now, by secondary seed tank alternate liquid in step (5) Body seed adds access fermentation tank, incubated 6h with 4% inoculum concentration；12 DEG C finally are to slowly warm up to 2 DEG C/h heating rates, Incubated 10h；Continue to be to slowly warm up to 38 DEG C with 2 DEG C/h heating rates, incubated 20h；

The fermentation medium forms：Maltodextrin 100g, corn flour 55g, bean powder 20g, wheat bran 12g, Chinese herbal medicine carry Take thing 40g, trehalose 35g, dusty yeast 6g, corn steep liquor 3g, ammonium sulfate 2g, dipotassium hydrogen phosphate 2g, potassium dihydrogen phosphate 2g, pure water L000mL, 6,121 DEG C of sterilizing 20min of pH value；

The ferment tank liquid cell concentration is 8.0x10⁹Individual/ml；

(7) zymotic fluid is concentrated in vacuo to the 45% of original volume through low-temperature negative-pressure, obtains bacterium concentrate, and concentrate is pressed with carrier Mass ratio is 0.5:1 uniformly mixing, in 45 DEG C of fluidized bed dryings, 40 DEG C of low-temperature grindings, grinding particle size 1mm, produces microorganism system Agent；

The vehicle weight number forms：CaCO₃22 parts, 12 parts of dextrin.

The microorganism formulation viable count content prepared through the above method is 3x10¹⁰Individual/g.

The preparation of the Chinese herbal medicine extract of embodiment 2

The Chinese herbal medicine extract is prepared by the raw material of following parts by weight：60 parts of Radix Angelicae Sinensis, 60 parts of Ligusticum wallichii, 55 parts of fructus amomi, 55 parts of the radix paeoniae rubrathe, 55 parts of cultivated land, 50 parts of Divine Comedy, 50 parts of malt, 50 parts of motherwort, 42 parts of costus root, 42 parts of Radix Codonopsis, 42 parts of rhizoma atractylodis, 42 parts of the Radix Astragali, 42 parts of honeysuckle, 40 parts of cordate houttuynia, 40 parts of Poria cocos, 40 parts of jujube kernel, 40 parts of fushen, 35 parts of polygala, fry fennel 35 Part, 35 parts of the bighead atractylodes rhizome, 35 parts of the bark of official magnolia, 30 parts of Fructus Aurantii, 30 parts of radix glycyrrhizae, 30 parts of hawthorn, 30 parts of dried orange peel.

The preparation method of the Chinese herbal medicine extract is：Said herbal medicine is crushed to particle diameter as less than 2 millimeters respectively, so After uniformly being mixed in container and adding the water of 5 times of weight, 80 DEG C of holding 3h of control temperature, 50 DEG C are then cooled to, is added mixed The mixing enzyme preparation of compound material gross weight 8% is digested, and is 6.2 with newborn acid for adjusting pH value, is digested 3h, finally add mixture Expect the mixture of 2 times of w ethanols (95%) and propyl alcohol, the mass ratio of ethanol and the propyl alcohol mixing is 1:1.5, control temperature To 70 DEG C of holding 4h, filtering；It is 25% that filtrate decompression, which is concentrated into solid content, and then freeze-drying, 40 DEG C of low-temperature grindings are Obtain Chinese herbal medicine extract；

The parts by weight of the mixed enzyme form：15 parts of endo-beta-glucanase, outer 15 parts of 1,4 beta-glucanase, β-glucose 12 parts of glycosides enzyme, 18 parts of zytase, 18 parts of pentosanase, 25 parts of Pullulanase, 12 parts of beta amylase, 12 parts of neutral proteinase, 12 parts of acid protease, 8 parts of superoxide dismutase, 8 parts of glucose oxidase, 8 parts of acid phosphatase；

The Chinese herbal medicine extract granularity prepared through above-mentioned preparation method is 1.2mm, and effective component extraction rate reaches more than 95.

The compounding of the complex enzyme of embodiment 3

The complex enzyme is uniformly mixed by the solid polypeptide formulation of following parts by weight：25 parts of neutral proteinase, it is acid 25 parts of protease, 25 parts of alkalescent xylanase, 25 parts of acidic xylanase, 25 parts of cellulase, 18 parts of 1,4 beta-glucanase, pectin 18 parts of enzyme, 18 parts of amylase, 12 parts of seminase, 12 parts of phytase.

Microorganism formulation, Chinese herbal medicine extract and the complex enzyme prepared respectively using embodiment 1-3 is raw material, with commercially available the third two Alcohol, glycerine, calcium propionate, alumino-silicate, niacinamide, nicotinic acid, sodium lactate, sodium chloride, citric acid, rumen glucose, rumen bypass Amino acid, rumen bypass choline, B B-complex, composite mineral matter are compounded, and prepare the grape that can improve cow rumen flora Sugar precursor thing, specific composition of raw materials are shown in Table 1, and preparation method is shown in embodiment 4-6；

The glycerine is food grade glycerin, purity 99.5%；

The alumino-silicate is to cross aluminate and its derivative with the strong suction-operated of selectivity；

The sodium lactate is 60% food grade lactic acid sodium；

The citric acid is solid citric acid；

The composition of raw materials of table 1

Embodiment 4

A kind of preparation method for the glucose precursor thing for improving cow rumen flora, comprises the following steps：

1) each raw material is accurately weighed according to above-mentioned formula rate, propane diols and glycerine is separately added into its quality 0.5 first Sterilized water again is diluted, and then mixes dilution, while heating water bath, while being stirred to obtain mixture A, is protected to 30 DEG C Temperature is stand-by；

5) mixture B is added into LDH-1 type coulter type mixers, starts mixer, be subsequently added into mixture A and uniformly mix 10min；Then mixture C is added, uniformly mixes 5min；Mixture D is eventually adding, uniformly mixes 3min, by final mixture Seal, pack and produce the glucose precursor thing that can improve cow rumen flora.

Embodiment 5

1) each raw material is accurately weighed according to above-mentioned formula rate, propane diols and glycerine is separately added into its quality 0.8 first Sterilized water again is diluted, and then mixes dilution, while heating water bath, while being stirred to obtain mixture A, is protected to 40 DEG C Temperature is stand-by；

5) mixture B is added into LDH-1 type coulter type mixers, starts mixer, be subsequently added into mixture A and uniformly mix 15min；Then mixture C is added, uniformly mixes 8min；Mixture D is eventually adding, uniformly mixes 4min, by final mixture Seal, pack and produce the glucose precursor thing that can improve cow rumen flora.

Embodiment 6

1) each raw material is accurately weighed according to above-mentioned formula rate, propane diols and glycerine is separately added into 1 times of its quality first Sterilized water be diluted, then dilution is mixed, while heating water bath is to 50 DEG C, while being stirred to obtain mixture A, is incubated It is stand-by；

5) mixture B is added into LDH-1 type coulter type mixers, starts mixer, be subsequently added into mixture A and uniformly mix 20min；Then mixture C is added, uniformly mixes 10min；Mixture D is eventually adding, uniformly mixes 5min, by final mixture Seal, pack and produce the glucose precursor thing that can improve cow rumen flora.

Glucose precursor thing application test prepared by the embodiment 5 of embodiment 7

1 materials and methods

1.1st, test material

1.1.1, product of the present invention

1.1.2, experiment milk cow：Experiment is carried out in a certain cattle farm in Miyun Region of Beijing, kind be the tire of china holstein cowses 2 with Upper Diseases of Cow, totally 46, wherein 16,2 tire, 3 12, tires, 4 10, tires, 58, tires.Test ox is given milk by parity, early stage Same or like pair principle is measured, is divided into experimental group and control group, every group 23.The upper parity milk cow annual output of milk is real Test a group 8454kg, control group 8533kg.

1.2nd, daily ration composition is tested：Test group and control group use same feeding and management method.Diet group turns into fine fodder (corn 52.7%, wheat bran 8%, dregs of beans 7%, cotton dregs 9%, peanut meal 9%, corn DDGS 8%, premix 7115A6%), slightly Expect for corn silage, sheep's hay, the beans stalks of rice, wheat, etc., clover etc..

1.3rd, test method：According to milk cow breed record, enter the expected date of childbirth before 15 longicorns be only randomly divided into test group with Two processing of control group.Control group is fed according to a conventional method, and test group is in addition to taking same method with control group and feeding, from production First 20 days to 30 days postpartum, this preparation 100g is taken per cow head daily, this preparation is a package metro-measuring per 100g, per natural gift It is fed for perinatal period compound nutritional pre-mixing agent 2 times.All milk production fine fodder 4-8kg for autogamy in examination Cow-feeding field, according to raising Stage and the output of milk are adjusted, corn silage quantitative feeding 20kg/ days, roughage (sheep's hay, the beans stalks of rice, wheat, etc., clover) free choice feeding.

1.4th, produce, be metabolized, reproductive performance record：The milk production of interior measure and record per cow head during 120 days postpartum Amount；Observation, evaluation milk cow body condition；Detect milk cow blood plasma fractions metabolite；Detect cow serum immunoglobulin content；Detection Reproductive performance；The rutting situation for milk cow of participating in the experiment is observed and recorded in during 120 days postpartum, it is whether pregnant with 60 days after breeding Examination per rectum is defined.

1.5th, disease surveillance and record：To Dairy Cows (mammitis, endometritis, ketoacidosis, cud displacement, it is stupid The diseases such as solidity apositia, puerperal fever and retention of afterbirth) detection, childbirth same day monitoring retention of afterbirth incidence, define afterbirth Not discharge person is retention of afterbirth within 12-24 hours.Ketosis of dairy cows is detected using Ketone benzene, breast is detected using CMT-Test reagents Fang Yan.

1.6th, statistical analysis is handled initial data with EXCEL softwares, then carries out variance point with SAS [11] method Analysis.

1.7th, end of day in November 10 in test period experimental period from 10 days -2011 April in 2011, lasts 214 days.

2. result of the test

Influence of the 2.1 glucose precursor things to milk cow body condition：

The part Body Condition Score standard of 2 milk cow of table 5

Score value	Vertebra portion	Flank	Buttocks both sides	Root of the tail both sides	Hipbone, ischium joint
						1	It is very prominent	Root root is visible	Excessive settlement	Crypts is very deep	It is very prominent
2	It is obvious prominent	It is most visible	It is obvious to sink	Crypts is obvious	It is obvious prominent
						3	It is somewhat prominent	It is a small number of visible	Somewhat it sink	Crypts is slightly aobvious	It is somewhat prominent
4	It is straight	Lose completely	It is straight	Crypts does not show	Do not show protrusion
						5	It is plentiful	It is plentiful	It is plentiful	It is plentiful	It is plentiful

The milk cow body condition visual examination appraisal result of table 3

Result above shows：100g/ (d heads) glucose precursor thing is added in Diseases of Cow TMR daily rations to milk cow The trend that Body Condition Score is significantly improved, digestion, absorption and metabolism of the milk cow to nutriment can be improved, may advantageously facilitate and Improve the synthesis of the framework materials such as vivo protein, fat, hence it is evident that the body condition of milk cow can be improved.

Influence of the 2.2 glucose precursor things to milk cow energy balance

The Diseases of Cow blood plasma fractions metabolite testing result of table 4

The energy balance of milk cow can be evaluated by determining blood plasma associated metabolites.Result of the test is shown, adds Portugal Grape sugar precursor thing can significantly improve the concentration of glucose in Diseases of Cow blood plasma, reduce free fatty and beta-hydroxybutyric acid Concentration, show that the satisfaction degree of glucose in Contents in Cows improves, the degree of decomposition of body fat reduces, milk cow employ body fat compared with It is few.The concentration of Postpartum Cows free fatty acids in plasma and beta-hydroxybutyric acid is (free less than the critical value of clinical ketoacidosis diagnosis simultaneously The μ Eq/L of aliphatic acid 500,1200 μm of ol/mL of beta-hydroxybutyric acid).I.e. can be with using the special nutrition regulation additive of Diseases of Cow Prevent the generation of milk cow negative energy balance.

Influence of the 2.3 glucose precursor things to milk production of cow

5120 days milk production of cow of table

	Head number (head)	Milk production number of days (my god)	Total output of milk (kg)	Average milk production (kg/ days heads)
					Control group	23	120	105898	38.37±5.16
Test group	23	120	123703	44.82±5.92
					Processing difference^b				6.45

Result above shows：Test group 6.45 kilograms of the output of milk of head day increase, improves 16.81%, t compared with control group Significant difference (P ＜ 0.05) is examined, glucose precursor thing is remarkably improved the milk yield of lactating cow.

Influence of the 2.4 glucose precursor things to immunity of cow

The Diseases of Cow Immunoglobulins in Serum testing result of table 6

Immunoglobulin IgA, IgM, IgG content are to judge the important finger of milk cow humoral immunity level in cow serum Mark.As seen from the experiment, cow birth stress cause serum immune globulin IgA, IgM, IgG content drastically to decline, body fluid Immunologic function is suppressed.Glucose precursor thing is used to increase by 1 week, childbirth before cow birth in Diseases of Cow daily ration 1 week serum IgA, IgM, IgG content after the same day and childbirth, strengthen humoral immune function.

Influence of the 2.5 glucose precursor things to Diseases of Cow reproductive performance

The Diseases of Cow reproductive performance testing result of table 7

Result of the test shows that Diseases of Cow can greatly shorten milk cow postpartum heat using glucose precursor thing Nearly 10 days of time, improve the conception rate 16.2% of postpartum the first feelings phase milk cow, the heat ratio of increase Postpartum Cows colony 16%, effectively improve the reproductive performance of milk cow.

The influence that Diseases of Cow postpartum disease occurs 2.6 glucose precursor things

The Diseases of Cow postpartum disease testing result of table 8

Result above shows：Test group in terms of postpartum disease of cow is prevented, can significantly reduce various compared with control group The incidence of disease of disease, 13.0-30.5% is averagely reduced, t examines significant difference (P ＜ 0.05), and glucose precursor thing can significantly drop The disease such as low milk cow postpartum mammitis, endometritis, ketoacidosis, cud displacement, intractable apositia, puerperal fever and retention of afterbirth Incidence, improve the health status of milk cow.

Claims

1. a kind of preparation method for improving cow rumen flora glucose precursor thing, it is characterised in that comprise the following steps：Accurately Weigh microorganism formulation 45-55 parts, propane diols 22-28 parts, glycerine 22-28 parts, calcium propionate 22-28 parts, alumino-silicate 22-28 Part, Chinese herbal medicine extract 18-22 parts, niacinamide 12-18 parts, nicotinic acid 12-18 parts, sodium lactate 10-15 parts, sodium chloride 10-15 parts, Complex enzyme 8-12 parts, citric acid 5-10 parts, rumen glucose 5-10 parts, rumen-bypass amino acid 3-8 parts, rumen bypass choline 5-8 Part, B B-complex 3-5 parts, composite mineral matter 2-4 parts；Propane diols and glycerine are separately added into 0.5-1 times of its quality first Sterilized water is diluted, and then mixes dilution, heating water bath to 30-50 DEG C, while be stirred mixture A, insulation are treated With；Calcium propionate, alumino-silicate, sodium lactate, sodium chloride, citric acid are uniformly mixed into obtain mixture B simultaneously, Chinese herbal medicine is extracted Thing, rumen glucose, rumen-bypass amino acid, rumen bypass choline, composite mineral matter uniformly mix to obtain mixture C, by microorganism Preparation, niacinamide, nicotinic acid, complex enzyme, B B-complex uniformly mix to obtain mixture D；Then mixture B is added into LDH-1 types Coulter type mixer, start mixer, be subsequently added into mixture A and uniformly mix 10-20min；Then mixture C is added, uniformly Mix 5-10min；Mixture D is eventually adding, uniformly mixes 3-5min, final mixture is sealed, packs to produce and can improve milk The glucose precursor thing of bovine rumen flora；

The microorganism formulation is to occupy cud clostridium (Clostridium ruminocolum) CGMCC NO.2125 and black song The blended fermentation of mould (Aspergillus niger) CGMCC NO.7927, low-temperature negative-pressure vacuum concentration, fluidized bed drying, low temperature Crush and be made；

The preparation method of the microorganism formulation comprises the following steps：Cud clostridium CGMCC NO.2125 and black song will be occupied first Mould CGMCC NO.7927 inclined-planes bacterial strain activation, expands culture to first class seed pot through one-level, two level, three-level seed respectively；Then By aspergillus niger CGMCC NO.7927 and occupy cud clostridium CGMCC NO.2125 first class seed pots liquid seeds by volume 1： 0.5-1 is uniformly mixed, and secondary seed tank alternate culture, alternate culture medium loading amount 240L, cultivation temperature are accessed with 10% inoculum concentration 33-39 DEG C, mixing speed 200-700r/m, ventilation (V/V) 1:1-3, incubation time 15-24h；Last secondary seed tank alternate Liquid seeds access fermentation tank, 28-30 DEG C of cultivation temperature, mixing speed 200-700r/m, ventilation (V/V) with 6% inoculum concentration 1:1-3, incubation time 15-20h, fermentation tank culture medium composition are：Maltodextrin 50-150g, corn flour 50-60g, bean powder 15- 25g, wheat bran 10-15g, Chinese herbal medicine extract 30-50g, trehalose 30-40g, dusty yeast 4-8g, corn steep liquor 1-5g, ammonium sulfate 1- 3g, dipotassium hydrogen phosphate 1-2g, potassium dihydrogen phosphate 1-2g, pure water l000mL, pH value 5-7,121 DEG C of sterilizing 20min；Then with 1- 2 DEG C/h rate of temperature fall slow cooling is to 10-15 DEG C, incubated 10-12h；Continue with 1-2 DEG C/h rate of temperature fall slow cooling extremely 2-5 DEG C, now, secondary seed tank alternate liquid seeds are added into access fermentation tank, incubated 6-8h with 4% inoculum concentration；Most 10-15 DEG C is to slowly warm up to 1-2 DEG C/h heating rates afterwards, incubated 10-12h；Continue slow with 1-2 DEG C/h heating rates 37-39 DEG C is warming up to, incubated 15-20h；Zymotic fluid is concentrated in vacuo to the 45% of original volume through low-temperature negative-pressure, and it is dense to obtain bacterium Contracting liquid, concentrate are 0.5 in mass ratio with carrier:1 uniformly mixing, in 45 DEG C of fluidized bed dryings, 40 DEG C of low-temperature grindings, crushes grain 0.5-1.5mm is spent, produces microorganism formulation.

2. improve the preparation method of cow rumen flora glucose precursor thing as claimed in claim 1, it is characterised in that described Vehicle weight number forms：CaCO₃20-23 parts, dextrin 10-12 parts.

3. improve the preparation method of cow rumen flora glucose precursor thing as claimed in claim 1, it is characterised in that described Microorganism formulation viable count content is 1.5x10¹⁰-3x10¹⁰Individual/g.

4. improve the preparation method of cow rumen flora glucose precursor thing as claimed in claim 1, it is characterised in that described Alumino-silicate is alumino-silicate or alumino-silicate derivatives.

5. improve the preparation method of cow rumen flora glucose precursor thing as claimed in claim 1, it is characterised in that described The preparation method of Chinese herbal medicine extract is：Accurately weigh Radix Angelicae Sinensis 55-65 parts, Ligusticum wallichii 55-65 parts, fructus amomi 50-60 parts, radix paeoniae rubrathe 50- 60 parts, cultivated land 50-60 parts, Divine Comedy 45-55 parts, malt 45-55 parts, motherwort 45-55 parts, costus root 40-45 parts, Radix Codonopsis 40- 45 parts, rhizoma atractylodis 40-45 parts, Radix Astragali 40-45 parts, honeysuckle 40-45 parts, cordate houttuynia 35-45 parts, Poria cocos 35-45 parts, jujube kernel 35- 45 parts, fushen's 35-45 parts, polygala 30-40 parts, fry fennel 30-40 parts, bighead atractylodes rhizome 30-40 parts, bark of official magnolia 30-40 parts, Fructus Aurantii 25-35 Part, radix glycyrrhizae 25-35 parts, hawthorn 25-35 parts, dried orange peel 25-35 parts；Said herbal medicine is crushed to particle diameter as less than 2 millimeters respectively, Then uniformly mixed in container and add the water of 3-6 times of weight, -90 DEG C of holding 2-4h of control temperature 70 C, be then cooled to 45-60 DEG C, the mixing enzyme preparation for adding mixed material gross weight 5-10% is digested, and is 5.5-6.8 with newborn acid for adjusting pH value, 2-4h is digested, finally adds 75% ethanol of 0.5-3 times of weight of mixed material and the mixture of propyl alcohol, the ethanol and propyl alcohol mix The mass ratio of conjunction is 1:1-2, control temperature to 60 DEG C of -78 DEG C of holding 3-4h, filtering；Filtrate decompression is concentrated into solid content More than 20%, then freeze-drying, 40 DEG C of low-temperature grindings produce the Chinese herbal medicine extract that grinding particle size is 0.5-2mm.

6. improve the preparation method of cow rumen flora glucose precursor thing as claimed in claim 5, it is characterised in that described The parts by weight of mixed enzyme form：Endo-beta-glucanase 10-20 parts, outer 1,4 beta-glucanase 10-20 parts, beta-glucosidase 10-15 parts, zytase 15-20 parts, pentosanase 15-20 parts, Pullulanase 20-30 parts, beta amylase 10-15 parts are neutral Protease 10-15 parts, acid protease 10-15 parts, superoxide dismutase 5-10 parts, glucose oxidase 5-10 parts are acid Phosphatase 5-10 parts.

7. improve the preparation method of cow rumen flora glucose precursor thing as claimed in claim 1, it is characterised in that described Complex enzyme is uniformly mixed by the solid polypeptide formulation of following parts by weight：Neutral proteinase 22-28 parts, acid protease 22- 28 parts, alkalescent xylanase 22-28 parts, acidic xylanase 22-28 parts, cellulase 22-28 parts, 1,4 beta-glucanase 15-20 Part, pectase 15-20 parts, amylase 15-20 parts, seminase 10-15 parts, phytase 10-15 parts.

8. improve the preparation method of cow rumen flora glucose precursor thing as claimed in claim 1, it is characterised in that described Per kilogram B B-complex includes vitamin A 2800mg, vitamin B1 3000mg, vitamin C 2000mg, riboflavin 1600mg, pantothenic acid 1900mg, vitamin B6 1800mg, vitamin B12 1200mg, inositol 5000mg, vitamin E 600mg, Vitamine D3 5500mg, Vitamin K3 4000mg；Its surplus is carrier, and the carrier is rice bran.

9. improve the preparation method of cow rumen flora glucose precursor thing as claimed in claim 1, it is characterised in that described Per kilogram composite mineral matter includes calcium 6000mg, phosphorus 4200mg, sodium 5100mg, copper 800mg, iron 1800mg, potassium 3800mg, manganese 1400mg, cobalt 1600mg, iodine 5000mg, magnesium 1450mg；Its surplus is carrier, and the carrier is rice bran.