CN103936870B - Method for shortening soaking time of corns by combining pressurization and compound enzymatic method - Google Patents
Method for shortening soaking time of corns by combining pressurization and compound enzymatic method Download PDFInfo
- Publication number
- CN103936870B CN103936870B CN201410169053.6A CN201410169053A CN103936870B CN 103936870 B CN103936870 B CN 103936870B CN 201410169053 A CN201410169053 A CN 201410169053A CN 103936870 B CN103936870 B CN 103936870B
- Authority
- CN
- China
- Prior art keywords
- pressurization
- soaking time
- semen maydiss
- soaking
- corn
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention relates to a method for shortening soaking time of corns in the corn starch production process by combining intermittent pulsed pressurization and a compound enzymatic method. Under the conditions that the pressure is 1.5-5MPa, the addition amount of protease into corn per gram is 400-1200IU, the addition amount of corns and cellulase into corn per gram is 20-40IU/g, the soaking temperature is 40-50DEG C and the soaking time lasts for 10-12h, the moisture content of the soaked corns is 42.3%-46.7%, the yield of corn starch is 73.9%-75.1%, the content of dry matters in a soaking solution is 4.5%-5.0%, and the content of proteins is 0.030-0.038mg/mL. The method overcomes the defects of the traditional soaking technology that the soaking time is long, the energy consumption is high, SO2 gas pollutes the environment and the like, the intermittent pulsed pressurization method and an enzymatic method are combined, the soaking time can be greatly shortened, the energy consumption can be reduced, the soaking water can be saved, the cost can be lowered, and the pollution can be decreased. Therefore, the extraction of corn starch has extremely important significance.
Description
Technical field
The invention belongs to corn deep processing field and in particular to a kind of using pulse pressure-charging and compound enzyme joint infusion method from
And the method shortening the corn soaking time during production of corn starch.
Background technology
At present, corn starch wet method processing technology is the main technique adopting in the world, involved in process
First procedure is corn soaking operation, soaks the quality of effect, the length of soak time directly affects finished product of starch in processing
With the yield of other side-products, quality and production cost.Traditional corn soaking technology commonly used sulfurous acid soak 36～48h,
There is the shortcomings of soak time is long, and production cost is high, low production efficiency, power consumption is high, wastewater discharge is big in the method, released simultaneously
Put so2Gaseous contamination environment, sulfurous acid has strong corrosion to act on it is therefore desirable to carry out soaking technology technology leather to equipment and pipeline
Newly.
Enzyme process wet-grinding technology and relative device is advantageous in that it decreases about 70% soak time than traditional soaking technology, and enzyme is permissible
The protein of wrapping starch in Semen Maydiss is fully dissolved, makes starch separate out to greatest extent, and the starch yield producing
It is better than with quality or be equivalent to traditional handicraft.
Pressurization soaking technology can improve diffusion velocity to reduce soak time, for example by reducing hydration time
It is pressurization 5min under l400psi that gunasekaran and farkas finds in pressure, and soak time can be made to be reduced to from 36h
12h, and pulse pressure-charging is a kind of neither power consumption and the good method of simple structure and low cost, the present invention first will interval
Formula pulse pressure-charging method is combined with enzymatic steeping, can greatly reduce the energy expenditure during soak extraction, save to soak and use
Water, reduces cost, reduces pollution.Therefore, the extraction to corn starch is extremely important.
Content of the invention
It is an object of the invention to provide a kind of technique combining immersion using intermittent pulse pressurization and compound enzyme, effectively contract
The short corn soaking time, thus realizing corn deep processing low cost, high efficiency, low energy consumption, the breakthrough of eco-friendly production technology,
Make China's corn deep processing industrial technology that major transformation to occur, strengthen the competition in international market for China's corn deep processing industry
Power, has preferable Social benefit and economic benefit.
Steps taken of the present invention is:
A) pretreatment before soaking: select full grains, free from insect pests, the Semen Maydiss no going mouldy, remove impurity, clean 3～5 times, drench
After doing overnight, put in multifunctional reaction still；
B) soak: by being that 1～2:1 adds after tap water with Semen Maydiss mass ratio, add albumen by 400～1200iu/g Semen Maydiss
Enzyme, adds cellulase by 20～40iu/g Semen Maydiss, stirs, cover polyfunctional reactant kettle cover；
C) open intermittent pulse pressure charging system, make multifunctional reaction still pressure rise to 1.5～5mpa, each pressurization time
20-50min, average supercharging 2-5 time, adjusted using chiller in steam generator and reacting kettle jacketing and in reactor, soak temperature
40～50 DEG C of degree, soaks 10～12h.
Protease described in said method is alkaline protease, acid protease or bromelain.
Effect detection after soaking by above-mentioned condition: corn content 42.3%～46.7% after soaking after measured, Semen Maydiss
Starch yield 73.9%～75.1%, dry matter content 4.5%～5.0% in soak, protein content 0.030～
0.038mg/ml.
Experimental technique according to the present invention:
1st, the mensure of Corn moisture content after soaking:
Remove the moisture on Semen Maydiss surface after soaking with absorbent paper, accurately weigh sample 2～5g, be placed in the title drying to constant weight
In measuring bottle, it is placed in 105 ± 2 DEG C of baking oven, be dried to constant weight, put in exsiccator, be cooled to room temperature, until in front and back twice not
Till 0.001g, calculating of weighing.
2nd, in soak dry matter content mensure:
Weigh a certain amount of sample (weight m weighing tinfoil box and loading sample tinfoil box with the tinfoil box of constant weight1With
m2), it is placed in 105 ± 2 DEG C of baking oven, dries to constant weight, put in exsiccator, be cooled to room temperature, weigh weight m3, (m3-m1/
M2-m1) × 100% content being dry.
3rd, in soak protein content mensure:
(1) preparation of Coomassie brilliant blue reagent: Coomassie brilliant blue g-250100mg is dissolved in 50ml95% ethanol, adds
100ml85%h3po4, with distilled water diluting to 1000ml, filter paper filtering.
(2) preparation of standard protein solution: pure Sanguis Bovis seu Bubali albumin, joined with 0.15mol/lnacl according to its purity
It is set to 100 μ g/ml protein solutions..
(3) making of standard curve, the data point making standard curve is as shown in table 1:
Table 1: make the data point of standard curve
With a595nmFor vertical coordinate, standard protein content be abscissa (six points are 10ug, 20ug, 30ug, 40ug, 50ug,
60ug), as shown in upper table data, in coordinate plot on X axis standard curve.
(4) mensure of protein content: take 2 10ml tool plug test tube a, b, put into 1ml0.15mol/ in a test tube
Lnacl, 5ml Coomassie brilliant blue reagent；Put in b test tube 0.9ml0.15mol/lnacl, 5ml Coomassie brilliant blue reagent and
0.1ml testing sample, place 2min after, blank is done with a test tube liquid, with 1cm optical path cuvette under 595nm colorimetric, record b
Optical density od of sample in test tube595nm, and content x of protein in testing sample is checked in by standard curve.
4th, the mensure of corn starch yield: the Semen Maydiss after taking 100g to soak, put in pulper with rotating speed 9400～
9500r/min carries out the corase grind of 5min, the screen filtration by 40# for the serosity, oversize distilled water flushing, and the serosity of gained exists
In pulper, 3min fine grinding is carried out with rotating speed 12000r/min, use distilled water flushing pulper, the mixed liquor of gained passes through 200#
Screen filtration separation fiber, the static 1h of the serosity after filtration, pour out supernatant, base section by add supernatant be transferred to ratio
It is 1.04 again.Adjust the serosity of proportion and remaining supernatant has been pumped into starch table with constant flow pump with 50ml/min respectively
On, put in exsiccator with plastic spatula collection starch and be dried, weigh, the starch weight on starch groove is determined.
5th, the mensure of proteinase activity: casein solution is put in 40 ± 0.2 DEG C of waters bath with thermostatic control, preheats 5min, take 4 examinations
Pipe, respectively adds 1ml enzyme liquid, takes one as blank tube, plus 2ml trichloroacetic acid, and other 3 pipes, as testing tube, respectively add 1ml cheese
Element, shakes up, 40 DEG C of insulation 10min, takes out test tube, and in 3 testing tubes, each addition 2ml trichloroacetic acid, adds 1ml cheese in blank tube
Element.Standing 10min, filters precipitation, respectively takes the na that 1ml filtrate adds 0.4mol/l respectively2co35ml, Folin reagent 1ml, at 40 DEG C
Colour developing 20min, surveys od value with blank tube zeroising at 680nm.Do three times parallel, average.According to below equation:
Vigor=a × k × 4/10 × the nu/g (ml) of protease
The average od value of a: sample parallel test；K: extinction constant；4: the cumulative volume of reaction reagent；10: during enzyme digestion reaction
Between；N: enzyme liquid dilutes general times
The invention has the beneficial effects as follows: can solve soak time length present in the processing of current corn starch (36～
48h), so2 is seriously polluted, long the production cycle, the problems such as energy resource consumption is high, realizes corn starch low cost, high efficiency, mental retardation
Consumption, eco-friendly production technology break through, and can effectively strengthen China's corn starch and downstream industry is competing in international market
Strive power, there is preferable Social benefit and economic benefit.
Specific embodiment
To further describe the present invention with reference to specific embodiment, advantages of the present invention and feature will be with description and
Apparent, but these embodiments are only exemplary, do not constitute any restriction to the scope of the present invention.
Embodiment 1:
Select full grains, free from insect pests, the Semen Maydiss granulate 3.5kg no going mouldy, remove impurity, cleaning is drenched for three times after doing overnight,
Put in 10l multifunctional reaction still, add after being 1:1 water with Semen Maydiss mass ratio, add in 1000iu/g Semen Maydiss ratio
(Novi believes hydrolysising protease 2.4l to alcalase2.4l, and Beijing Gao Ruisen Science and Technology Ltd. produces；Add by 20iu/g Semen Maydiss
Cellulase (production of Zhaodong Cheng enzyme preparation company limited), stirs, and adjusting impulse pressure charging system makes reactor pressure liter
To 2mpa atmospheric pressure, interval supercharging 3 times, adjust the chiller in steam generator and reactor and keep soaking liquid temp 45
DEG C, after soaking 10h, shed pressure, open reactor lid, take out the Semen Maydiss soaking and soak.Record jade after immersion respectively
Rice moisture is 42.7%, dry matter content 4.82% in soak, and in soak, protein content is 0.036mg/ml, forms sediment
Powder yield 74.87%.
Embodiment 2:
Select full grains, free from insect pests, the Semen Maydiss granulate 3.5kg no going mouldy, remove impurity, cleaning is drenched for three times after doing overnight,
Put in 10l multifunctional reaction still, add the acid after being 1:1 water with Semen Maydiss mass ratio, adding in 1000iu/g Semen Maydiss ratio
Property protease (production of Ningxia jade of the He family Bioisystech Co., Ltd), by 20iu/g Semen Maydiss add cellulase (Zhaodong day become enzyme
Preparation company limited produces), stir, adjusting impulse pressure charging system makes reactor pressure be raised to 2mpa atmospheric pressure, and interval increases
Pressure 3 times, the chiller adjusting in steam generator and reactor keeps soak temperature 45 C, after soaking 10h.Shed pressure
Power, opens reactor lid, takes out the Semen Maydiss soaking and soak.After recording immersion respectively, Corn moisture content is 45.1%,
Dry matter content 4.54% in soak, in soak, protein content is 0.032mg/ml, starch yield 74.71%.
Embodiment 3:
Select full grains, free from insect pests, the Semen Maydiss granulate 3.5kg no going mouldy, remove impurity, cleaning is drenched for three times after doing overnight,
Put in 10l multifunctional reaction still, add after being 1:1 water with Semen Maydiss mass ratio, add Fructus Ananadis comosi in 1200iu/g Semen Maydiss ratio
Protease (Pangbo Bioengineering Co Ltd, Nanning's production), (Zhaodong day becomes enzyme preparation to add cellulase by 20iu/g Semen Maydiss
Company limited produces), stir, adjusting impulse pressure charging system makes reactor pressure be raised to 2mpa atmospheric pressure, interval supercharging 3
It is secondary, the chiller adjusting in steam generator and reactor keeps soak temperature 45 C, after soaking 15h.Shed pressure,
Open reactor lid, take out the Semen Maydiss soaking and soak.After recording immersion respectively, Corn moisture content is 46.3%, soaks
Dry matter content 4.75% in liquid, in soak, protein content is 0.035mg/ml, starch yield 73.90%.
Claims (8)
1. the method that combined-enzyme method shortens the corn soaking time is worked in coordination with a kind of pressurization, and its step is as follows:
A) pretreatment before soaking: select full grains, free from insect pests, the Semen Maydiss no going mouldy, remove impurity, clean 3～5 times, drench and did
After night, put in multifunctional reaction still；
B) soak: by being that 1～2:1 adds after tap water with Semen Maydiss mass ratio, add protease by 400～1200iu/g Semen Maydiss,
Add cellulase by 20～40iu/g Semen Maydiss, stir, cover polyfunctional reactant kettle cover；
C) pressurize: open intermittent pulse pressure charging system, make multifunctional reaction still pressure rise to 1.5～5mpa, each pressurization time
20～50min, average supercharging 2～5 times, adjusted in reactor using chiller in steam generator and reacting kettle jacketing and soak
40～50 DEG C of temperature, soaks 10～12h.
2. as claimed in claim 1 a kind of collaborative combined-enzyme method of pressurization shorten the corn soaking time method it is characterised in that:
It is to put into 3.5kg Semen Maydiss in 10l multifunctional reaction still.
3. as claimed in claim 1 a kind of collaborative combined-enzyme method of pressurization shorten the corn soaking time method it is characterised in that:
It is to add protease by 1000iu/g Semen Maydiss.
4. as claimed in claim 1 a kind of collaborative combined-enzyme method of pressurization shorten the corn soaking time method it is characterised in that:
It is to add cellulase by 20iu/g Semen Maydiss.
5. as claimed in claim 1 a kind of collaborative combined-enzyme method of pressurization shorten the corn soaking time method it is characterised in that:
Multifunctional reaction still pressure is 2mpa.
6. as claimed in claim 1 a kind of collaborative combined-enzyme method of pressurization shorten the corn soaking time method it is characterised in that:
Soaking temperature in reactor is 45 DEG C.
7. as claimed in claim 1 a kind of collaborative combined-enzyme method of pressurization shorten the corn soaking time method it is characterised in that:
Soak time in reactor is 10h.
8. the method that combined-enzyme method shortens the corn soaking time is worked in coordination with a kind of pressurization as described in claim 1-7 any one,
It is characterized in that: protease is alkaline protease, acid protease or bromelain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410169053.6A CN103936870B (en) | 2014-04-25 | 2014-04-25 | Method for shortening soaking time of corns by combining pressurization and compound enzymatic method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410169053.6A CN103936870B (en) | 2014-04-25 | 2014-04-25 | Method for shortening soaking time of corns by combining pressurization and compound enzymatic method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103936870A CN103936870A (en) | 2014-07-23 |
| CN103936870B true CN103936870B (en) | 2017-01-25 |
Family
ID=51184771
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410169053.6A Active CN103936870B (en) | 2014-04-25 | 2014-04-25 | Method for shortening soaking time of corns by combining pressurization and compound enzymatic method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103936870B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109907154A (en) * | 2019-04-19 | 2019-06-21 | 安徽丰原发酵技术工程研究有限公司 | A kind of method of corn soaking new process preparation food-grade corn albumen |
| CN109907329A (en) * | 2019-04-19 | 2019-06-21 | 安徽丰原发酵技术工程研究有限公司 | A kind of method of corn soaking new process preparation food-grade corn fiber |
| CN109907237A (en) * | 2019-04-19 | 2019-06-21 | 安徽丰原发酵技术工程研究有限公司 | A kind of method of corn soaking new process preparation food-grade corn plumule |
| CN109938258A (en) * | 2019-04-19 | 2019-06-28 | 安徽丰原发酵技术工程研究有限公司 | A kind of corn soaking new process |
| CN112457419B (en) * | 2020-11-23 | 2023-03-21 | 鲁洲生物科技（山东）有限公司 | Method for shortening corn soaking time by using tumbling technology in cooperation with sulfurous acid |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101372702A (en) * | 2008-10-21 | 2009-02-25 | 吉林农业大学 | Method for shortening maize immersion time in cornstarch production process |
| CN101607218B (en) * | 2009-07-17 | 2012-05-23 | 中国科学院过程工程研究所 | Corn steam explosion separation and endosperm multi-component co-production utilization technology thereof |
| CN101628920B (en) * | 2009-08-17 | 2011-12-14 | 福州大学 | Comprehensive utilization method of corn cob |
-
2014
- 2014-04-25 CN CN201410169053.6A patent/CN103936870B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN103936870A (en) | 2014-07-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103936870B (en) | Method for shortening soaking time of corns by combining pressurization and compound enzymatic method | |
| CN101372702A (en) | Method for shortening maize immersion time in cornstarch production process | |
| CN102432022B (en) | Method for producing ultra-pure quartz sand by vein quartz and high-temperature chlorination device | |
| CN106334529A (en) | Quaternary ammonium salt cationic kieselguhr and preparation method thereof | |
| CN104340982A (en) | Production technique for preparing silica white from rice hull ash | |
| CN104263708B (en) | Higher-heat-stability amylase mutant, and preparation method and application thereof | |
| CN109037028B (en) | Silicon material cleaning method | |
| CN103396431A (en) | Method for continuously hydrolyzing organic silicon high-boiling residues | |
| CN109134706A (en) | A method of promoting agar whiteness | |
| CN102838230B (en) | Method utilizing cation exchange resin to pretreat citrus processing wastewater | |
| Li et al. | Optimized strategy for simultaneous recovering bioactive oligosaccharides and reusable perlite from agar industrial waste residues | |
| CN102041216A (en) | Yellow wine production method with improved rice raw material treatment | |
| CN105062736B (en) | A kind of method of efficiently saccharifying cornstarch in wheat juice preparation process | |
| CN107540757A (en) | A kind of method that enzyme process auxiliary carragheen decolourizes | |
| CN101491353B (en) | Environmental protection synthesis preparation method of bioactive substance in shrimp shell | |
| CN102363625B (en) | Malt root resource comprehensive utilization method | |
| CN106749762A (en) | A kind of preparation method of chitosan hydrochloride | |
| CN112126640A (en) | Neutral urease immobilization and application thereof in degradation of yellow wine urea | |
| CN102775524A (en) | Method for directly producing agarose from alga | |
| CN114409598A (en) | Preparation method of ionic liquid 1-methyl 3- (4-sulfobutyl) imidazole bisulfate | |
| CN109298130A (en) | The detection method of starch in a kind of high sugared dried meat floss | |
| CN101200292B (en) | Hydrophobic white carbon black preparing method | |
| CN108855000B (en) | Preparation method of nano adsorption material | |
| CN211412010U (en) | Ionic liquid catalyst synthesis device for producing dimethyl carbonate by ester exchange method | |
| CN110195046A (en) | A kind of cellulase and preparation method thereof of bagasse fermentation residue production |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |