CN103931648B - The peanut seed coat agent of a kind of aspergillus flavus-resistance mould - Google Patents

The peanut seed coat agent of a kind of aspergillus flavus-resistance mould Download PDF

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Publication number
CN103931648B
CN103931648B CN201410138298.2A CN201410138298A CN103931648B CN 103931648 B CN103931648 B CN 103931648B CN 201410138298 A CN201410138298 A CN 201410138298A CN 103931648 B CN103931648 B CN 103931648B
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aspergillus flavus
seed coat
coat agent
agent
peanut seed
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CN103931648A (en
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齐宏涛
于丽娜
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Shandong Peanut Research Institute
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Qingdao Bo Zhiyuan Bioisystech Co Ltd
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Abstract

The present invention relates to a kind of seed coat agent of Resistance to Aspergillus flavus invasion, formulated through processing by non-active ingredients such as insecticide, aspergillus flavus resisting microbial inoculum, Activities of Plant Growth Regulators composition and film forming agent, emulsifier, dispersant, trace element fertilizer, antifreezing agent, warning colouration, defoamers.Wherein, insecticide be Imidacloprid, Diacloden, cough up in bacterium eyeball, phoxim, carbosulfan, fenpiclonil any one; Aspergillus flavus resisting microbial inoculum is citral; Plant growth regulator is any combination of any one or they in paclobutrazol, naa, heteroauxin, indolebutyric acid.Product of the present invention, except having good pest-resistant effect, also has the effect of good aspergillus flavus-resistance mould, thus effectively suppresses Aspergillus flavus infecting peanut, reduces aflatoxin pollution of peanuts risk, ensures peanut food safety.

Description

The peanut seed coat agent of a kind of aspergillus flavus-resistance mould
Art
The present invention relates to agricultural seed treatment field; Be specifically related to the peanut seed coat agent of a kind of aspergillus flavus-resistance mould.
Background technology
Aflatoxin contamination is the important limiting factor of restriction China Peanut Industry Development.The secondary metabolite of aflatoxin mainly Aspergillus flavus and aspergillus parasiticus bacterium, has high carcinogenic and high toxicity.Peanut is as the crop of geocarpic of blooming on the ground, be easy to be subject to infecting of Aspergillus flavus in soil, the aspergillus flavus that research shows in peanut caused by mainly soil Aspergillus flavus is infected, therefore prevents the Synthetical prevention that in soil, Aspergillus flavus is infected for aflatoxin pollution of peanuts peanut pod particularly important.
Citral system geranial (trans first position citral, and neral (cis second position lemon rubber aldehyde geranial), neral) mixture, colourless or micro-yellow liquid, in strong Lemon fruit fragrance, by separation and purification in litsea cubeba oil or lemon rubber grass oil, get Shi China allows the food additives used.Citral can destroy the cell wall of aspergillus flavus and the structure of plasma membrane, and changes its permselectivity, and impact is to its absorbing nutriment; The infiltration of citral; cause the change of structure of mitochondria and irregular hyperplasia, normal oxidation-reduction system is destroyed, and during itself and the acid of three shuttles are circulated, the vigor of related enzymes weakens; thus produce the reduction of respiratory rate, reduce the generation of energy and the utilization to oxygen; By making mitochondria and core DNA damage, mycelial cell is dead, an armful son loses germination and is killed.
Citral is applied in peanut seed coat agent and is used for aspergillus flavus-resistance mould dip-dye aspect and still belongs to blank both at home and abroad.
The seed coat agent Main Function produced both at home and abroad is at present the effect of preventing disease pest control and seed health care, in aspergillus flavus-resistance mould, not yet have product.Peanut as blooming on the ground, the crop of geocarpic, its pod is easy to be subject to infecting of Aspergillus flavus in soil, causes the pollution of aflatoxin.Therefore the seed coat agent developing aspergillus flavus-resistance mould is most important for control aflatoxin contamination.
Summary of the invention
Object of the present invention is exactly for the deficiencies in the prior art, provides one and to cultivate peanut seed coat agent, make it effectively can not only control insect pest, the disease of crop growth period, and can effectively suppress Aspergillus flavus infecting peanut, ensures peanut food safety.
Seed coat agent provided by the present invention is as film forming agent with water-fast filmogen, mix with the inactive ingredient such as emulsifier, dispersant, trace element, antifreezing agent, defoamer, warning colouration and insecticide, aspergillus flavus resisting microbial inoculum, plant growth regulator isoreactivity composition, obtain through processing preparation.
Concrete component of the present invention and content are:
Insecticide can be in following agricultural chemicals any one: Imidacloprid, Diacloden, cough up bacterium eyeball, phoxim, carbosulfan, fenpiclonil, content is 0.3-16%.
Insecticide is selected from: Imidacloprid, Diacloden, the one coughed up in bacterium eyeball, phoxim, carbosulfan, fenpiclonil, content is 0.3-16%.
Aspergillus flavus resisting microbial inoculum: citral, content is 0.3-10%.
Plant growth regulator is selected from: any one or both any combination between them in paclobutrazol, naa, heteroauxin, indolebutyric acid, total content is 0.01-0.3%.
Trace element is selected from: zinc fertilizer, manganese fertilizer and boron fertilizer, and total content is 0.8-9%.Three kinds of fertilizer consumption separately can be had any different according to the soil regime of different regions, and such as lack zinc area, zinc fertilizer should strengthen, and boron fertilizer consumption should be relatively less under normal circumstances.
Film forming agent is selected from: the mixture of a kind of in polyvinyl acetate, methylcellulose, polyvinyl alcohol, polyethylene glycol, polyethylene glycol, polyvinylpyrrolidone, shellac, carboxymethyl cellulose and sodium alginate or two kinds, total consumption is 3-14%.
Emulsifier is selected from: the one in agriculture breast 500#, Nongru-700 #, agriculture breast OX-656, agriculture breast OX-653, agriculture breast OX-6667, agriculture breast 0201B, agriculture breast NP-7, agriculture breast S-80 or two kinds of mixtures, total consumption is 1-9%.
Dispersant is selected from: sodium lignin sulphate, calcium lignosulfonate, condensation compound of methyl naphthalene sulfonic acid and formaldehyde, naphthalene sulfonic acid-formaldehyde condensation product, methylene be a kind of in sulfonic acid, oleic acid methylaminoethyl sodium sulfonate, dibutyl naphthalenesulfonic acid formaldehyde condensation products or the mixture of two kinds how, and total amount is 1-9%.
Antifreezing agent is selected from: antifreezing agent is any one in ethylene glycol, glycerine, urea, and content is 2-9%.
Defoamer is selected from: the one in bubble enemy, silicone, saturated fat acids and acid amides.
Warning colouration is selected from: the one in acid red G, disperse red FB, basic rhodamine, and content is 0.2-1.0%.
Water: by weight percentage, complements to 100%.
After seed coat agent of the present invention carries out dressing to peanut seed, film forming agent in seed coat agent is formed at the surface of the seed to be had the water insoluble of fenestra road but can breathe freely, permeable good clothing film, thus by insecticide, aspergillus flavus resisting microbial inoculum, plant growth regulator isoreactivity composition and other inactive ingredient anastomose are together, after planting, under the effect of hydrone, active ingredient in clothing film is discharged by pole, fenestra road and lentamente progressively with the surface of the seed and close on soil and contact, thus kill kind of the Aspergillus flavus passed and an insect pest, and protective barrier is formed around it, cause the Aspergillus flavus around it, worm is difficult to existence, reach the object of permanently effective control Aspergillus flavus and underground pests etc.Because the efficacy of a drug is concentrated, availability is high, directly contacts with soil in addition, and be not subject to expose to the sun and rain and the impact of high temperature, thus the drug effect phase reaches 45-80 days, and vegetative period substantially need not dispenser again, saving of work and time, thus reduces the pollution to environment.In addition, plant growth regulator-methyl α-naphthyl acetate, indolebutyric acid, heteroauxin etc. in clothing film can also promote seed germination, promote root growth, improve emergence rate and resistance and planting percent.Micro-zinc fertilizer in clothing film, manganese fertilizer, boron fertilizer can make up soil fertility deficiency again, meet the trace element needed for rice shoot normal growth, effectively control nutritional deficiency disease.Seed coat agent using method of the present invention is conventional seed coat agent coating method.
The present invention compared with prior art has the following advantages: 1, because seed coat agent series of products of the present invention select citral to do aspergillus flavus-resistance mould and other germ agent, the antibacterial ability of the seed coat agent not only improved, and decrease the consumption of seed coat agent, reduce cost; 2, the present invention's series seed coat agent product peel is strong, and curing rate is fast, can control some seed with sick worm propagate, the particularly propagation of Aspergillus flavus; 3, the adding of low toxic pesticide, overcome the defect that the desinsection not sterilization existing for seed coat agent done by simple use of insecticide, make it have the dual-use function of desinsection, sterilization; 4, the present invention's series seed coat agent product is nontoxic to seed, harmless, easy to use, safe and reliable, the test of pesticide effectiveness proves, with seed coat agent product of the present invention, all there is higher anti-efficiency to the damage by disease and insect of peanut, as can more than 80% be reached to the control rate of aspergillus flavus bacterium, greatly reduce the quantity of Aspergillus flavus in peanut, can more than 85% be reached to the anti-efficiency of soil insect, imitate the lasting period can reach about 40 days, be more conducive to the generation of pest control.
Production method of the present invention is the production method of agricultural chemicals suspension agent general at present, and its poly-adopted operating procedure is as follows:
According to the material sand ratio (volume ratio) of 1:1.5, a certain amount of abrasive body bead is first dropped in sand milling still, press recipe requirements again, by raw material, antifreezing agent, add in raw material blending tank after dispersant and water metering, start high-shear emulsion machine, after stirring corase grind, re-use skin grinder and carry out fine grinding, optionally defoamer is added in frosted process, after frosted to certain hour, sampling and measuring granularity, after granularity meets standard, be frosted to terminal, static 30min, bead is landed, then with vacuum tube, slip is drawn in modulation kettle, film forming agent is added according to amount of liquid medicine, warning colouration, stir 30min, the active constituent content of sample analysis product, proportion, viscosity, film formation time, expulsion rate, the technical indicators such as pourability, after meeting product standard, carry out metering packing.

Claims (8)

1. a peanut seed coat agent for aspergillus flavus-resistance mould, containing inactive ingredient such as insecticide, aspergillus flavus resisting microbial inoculum and film forming agent, emulsifier, dispersant, antifreezing agent, defoamer, warning colouration and trace elements.It is characterized in that: insecticide can be in following agricultural chemicals any one: Imidacloprid, Diacloden, cough up bacterium eyeball, phoxim, carbosulfan, fenpiclonil, content is 0.3-16%; Aspergillus flavus resisting microbial inoculum is citral, and content is 0.3-10%; Plant growth regulator can be any one or both any combination between them in following agricultural chemicals: paclobutrazol, naa, heteroauxin, indolebutyric acid, total content is 0.01-0.3%.
2. the peanut seed coat agent of a kind of aspergillus flavus-resistance mould according to claim 1, it is characterized in that: film forming agent can be the mixture of a kind of in polyvinyl acetate, methylcellulose, polyvinyl alcohol, polyethylene glycol, polyethylene glycol, polyvinylpyrrolidone, shellac, carboxymethyl cellulose and sodium alginate or two kinds, and total consumption is 3-14%.
3. the peanut seed coat agent of a kind of Aspergillus flavus according to claim 1, it is characterized in that: emulsifier can be agriculture breast 500#, one in Nongru-700 #, agriculture breast OX-656, agriculture breast OX-653, agriculture breast OX-6667, agriculture breast 0201B, agriculture breast NP-7, agriculture breast S-80 or two kinds of mixtures, total consumption is 1-9%.
4. the peanut seed coat agent of a kind of Aspergillus flavus according to claim 1, it is characterized in that: dispersant can be a kind of in sodium lignin sulphate, calcium lignosulfonate, condensation compound of methyl naphthalene sulfonic acid and formaldehyde, naphthalene sulfonic acid-formaldehyde condensation product, methylene how sulfonic acid, oleic acid methylaminoethyl sodium sulfonate, dibutyl naphthalenesulfonic acid formaldehyde condensation products or two kinds mixture, and total amount is 1-9%.
5. the peanut seed coat agent of a kind of aspergillus flavus-resistance mould according to claim 1, is characterized in that: antifreezing agent is any one in ethylene glycol, glycerine, urea, and content is 2-9%.
6. the peanut seed coat agent of a kind of aspergillus flavus-resistance mould according to claim 1, is characterized in that: defoamer can for the one in bubble enemy, silicone, saturated fat acids and acid amides.
7. the peanut seed coat agent of a kind of aspergillus flavus-resistance mould according to claim 1, is characterized in that: warning colouration can for the one in acid red G, disperse red FB, basic rhodamine, and content is 0.2-1.0%.
8. the peanut seed coat agent of a kind of aspergillus flavus-resistance mould according to claim 1, is characterized in that: trace element can be zinc fertilizer, manganese fertilizer and boron fertilizer, and total content is 0.8-9%.
CN201410138298.2A 2014-04-09 2014-04-09 The peanut seed coat agent of a kind of aspergillus flavus-resistance mould Active CN103931648B (en)

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CN105028633A (en) * 2014-12-09 2015-11-11 山东省花生研究所 Method of resisting aspergillus flavus infection on peanut with citral-chitosan composite coating
CN104430833A (en) * 2014-12-19 2015-03-25 山东营养源食品科技有限公司 Fruit and vegetable fresh-keeping agent
CN106614752A (en) * 2016-11-14 2017-05-10 东莞市联洲知识产权运营管理有限公司 Peanut seed coating agent and preparation method thereof
CN107085066B (en) * 2017-04-06 2019-08-13 中国农业科学院油料作物研究所 A kind of pre-treating method for LC-MS detection Aspergillus flavus metabolism group
CN109362522A (en) * 2018-11-16 2019-02-22 山东省花生研究所 A kind of peanut cultivation method
CN109287642A (en) * 2018-11-16 2019-02-01 山东省花生研究所 One cultivates peanut antibacterial insect prevention seed coat agent

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Inventor after: Yu Lina

Inventor after: Qi Hongtao

Inventor before: Qi Hongtao

Inventor before: Yu Lina

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Effective date of registration: 20200921

Address after: 266000 No. 126 floating Hill Road, Licang District, Shandong, Qingdao

Patentee after: Shandong Peanut Research Institute

Address before: 266001 Shandong city of Qingdao province Huashan City Road 2, unit 4, 603 households

Patentee before: QINGDAO BOZHIYUAN BIOTECHNOLOGY Co.,Ltd.