CN103931615A - Preparation method of film coating agent and film sealing method for plant branch cut - Google Patents

Preparation method of film coating agent and film sealing method for plant branch cut Download PDF

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Publication number
CN103931615A
CN103931615A CN201410131952.7A CN201410131952A CN103931615A CN 103931615 A CN103931615 A CN 103931615A CN 201410131952 A CN201410131952 A CN 201410131952A CN 103931615 A CN103931615 A CN 103931615A
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filming agent
weight
plant material
suitable multiple
pivot weight
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CN201410131952.7A
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CN103931615B (en
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吴中旺
周学斌
邹文武
吴峰
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SHENZHEN XINHUA NANFANG BIOTECHNOLOGY Co Ltd
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SHENZHEN XINHUA NANFANG BIOTECHNOLOGY Co Ltd
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Priority to CN201410131952.7A priority Critical patent/CN103931615B/en
Publication of CN103931615A publication Critical patent/CN103931615A/en
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Abstract

The invention discloses a preparation method of a film coating agent and a film sealing method for a plant branch cut. The preparation method comprises the following steps: step 1. dissolving nutrient components of which the weight is a proper times the standard weight by use of a proper amount of corresponding solvent; d step 2. adding a film formation component of which the weight is a proper times the standard weight and the dissolved nutrient components into water and stirring uniformly to obtain the film coating agent. The film sealing method comprises the following steps: firstly, putting the film coating agent with required weight into a container, diluting to a required concentration by use of water, stirring fully and uniformly, and clipping a plant material to be with required length and angle; secondly, respectively dipping the two ends of the clipped plant material in the diluted film coating agent for one time, and sealing the two ends of the plant material dipped in the film coating agent by use of a preservative film; and finally, packaging and bundling the sealed plant material by use of leatheroid. The preparation method disclosed by the invention has disinfection and sterilization functions and is safe, convenient and rapid to operate, convenient to use, long in preservation period, wide in applicability, simple and easy to learn, and easy to popularize.

Description

A kind of filming agent preparation method and plants shoots incision film encapsulation method
Technical field
The present invention relates to a kind of preparation method of filming agent, also relate to a kind of method of plants shoots otch being carried out to film envelope.
Background technology
Grafting, has become the some effective that current plant changes breeding, raising output, increases Social benefit and economic benefit.But, in actual grafting operation, also there are many technical problems urgently to be resolved hurrily.For example, the otch of grafting twig easily makes branch moisture scatter and disappear fast, makes branch withered, greatly reduces graft survival rate.In the time of needs long distance transportation grafting twig, because the fresh-keeping problem of grafting twig is difficult to solve, greatly restrict promoting the use and developing of graft technology.In order to address this problem, occurred the technology of sealing with wax, but there is following defect in the method:
First, the method requires wax is dissolved and maintain the temperature between 80 DEG C~90 DEG C, and excess Temperature easily scorches branch; The too low wax of temperature dissolves and not exclusively easily causes wax layer gage distortion, and when paraffin removal, fragile branch, bud head cause branch all to be scrapped.The method is because wax temperature in practical operation is difficult to control, operation easier is difficult to greatly promote.
Secondly, the method can only be sealed grafting twig otch, there is no disinfecting and sterilizing functions, easily breeds the plant of bacterium for otch, is difficult to meet to the carry out disinfection requirement of sterilization of branch otch.For example, the little seeds of a tung oil tree belong to half meat euphorbia plant, and its root, stem, leaf all contain a large amount of protein type milk, cut off rear milk and flow out in a large number, very easily cause bacterial reproduction to infect and cause that branch rots, causing that branch exceedes injured tissue, the function of taking root lost efficacy and caused that survival rate is low.
Summary of the invention
One of the technical problem to be solved in the present invention is, a kind of filming agent preparation method is provided, and for grafting twig otch provides a kind of film closure material, overcomes the defect such as difficult control of temperature, operation requirements height that the existing technology of sealing with wax exists.
Two of the technical problem to be solved in the present invention is, a kind of plants shoots incision film encapsulation method is provided, overcome existing seal with wax technology exist difficult control of temperature, operation requirements high, without defects such as disinfecting and sterilizing functions.
The present invention solves the technical scheme that one of its technical problem adopts: a kind of filming agent preparation method is provided, it is characterized in that, comprise the steps:
A1, dissolving nutrition composition: by appropriate corresponding dissolution with solvents for the nutrition composition of pivot weight suitable multiple; Water-soluble liquid nutrition composition is directly used;
A2, the nutrition composition of the film-forming components of pivot weight suitable multiple and this dissolving is added to the water and is stirred, obtain filming agent.
In filming agent preparation method of the present invention, described nutrition composition is indolebutyric acid, heteroauxin, indolebutyric acid potassium, brassin, the basic element of cell division, kinetin, CPPU one or a combination set of, wherein, the solvent that indolebutyric acid, heteroauxin, indolebutyric acid potassium, brassin and the basic element of cell division are corresponding is that concentration is more than 80% ethanol, to the suitable multiple of pivot weight is: 2~10 times of indolebutyric acids, 5~30 times of heteroauxins, 2~10 times, indolebutyric acid potassium, 20~30 times of brassins, 1~2 times of the basic element of cell division; The solvent that kinetin is corresponding is glacial acetic acid; Kinetin is 3~4 times to the suitable multiple of pivot weight; The solvent that CPPU is corresponding is methyl alcohol; CPPU is 0.3~1 times to the suitable multiple of pivot weight;
Described film-forming components is mill taro powder, modified corn starch, concentration shitosan that is 5%~10% one or a combination set of, wherein, to the suitable multiple of pivot weight is: shitosan is appropriate, 50~100 times, mill taro powder, 50~100 times of modified corn starch.
In filming agent preparation method of the present invention, filming agent wet weight is pivot weight 500~1200 times.
In filming agent preparation method of the present invention, steps A 1 comprises: dissolve antioxidant, by appropriate corresponding dissolution with solvents for the antioxidant of pivot weight suitable multiple; Steps A 2 comprises: add the antioxidant of the dissolving of steps A 1 to stir, obtain filming agent.
In filming agent preparation method of the present invention, described dissolving antioxidant is d ascorbic acid, and the solvent that d ascorbic acid is corresponding is water, and d ascorbic acid is 30~50 times to the suitable multiple of pivot weight.
In filming agent preparation method of the present invention, described steps A 1 comprises dissolving bactericidal component: by appropriate corresponding dissolution with solvents for the bactericidal component of pivot weight suitable multiple; Described steps A 2 comprises: add the bactericidal component of the dissolving of steps A 1 to stir, obtain filming agent;
Or described steps A 1 comprises liquid fungicide, use the liquid fungicide of pivot weight suitable multiple; Described steps A 2 comprises: add the liquid fungicide of steps A 1 to stir, obtain filming agent.
In filming agent preparation method of the present invention, described bactericidal component is S-Ethyl ethylthio sulfonate, and the solvent that S-Ethyl ethylthio sulfonate is corresponding is ethanol, and S-Ethyl ethylthio sulfonate is 2~10 times to the suitable multiple of pivot weight;
Or described bactericidal component is little seeds of a tung oil tree based bacteriostat, little seeds of a tung oil tree based bacteriostat is 2~10 times to the suitable multiple of pivot weight.
The present invention solves the technical scheme that two of its technical problem adopts: a kind of plants shoots incision film encapsulation method is provided, it is characterized in that, comprise the steps:
S1, need put into container with the filming agent of weight and be diluted with water to and need concentration, stir; Plant material is trimmed to and needs length and angle;
S2, above-mentioned dilution is put into respectively in the plant material two ends of having pruned the filming agent libation at an ancient wedding ceremony once, the plant material two ends of the good filming agent of the libation at an ancient wedding ceremony are sealed with preservative film;
S3, the plant material paper using suitcase dress of good seal is tied up.
In plants shoots incision film encapsulation method of the present invention, in step S2, first by bundled the plant material of having pruned quantity binding on demand, then by the filming agent of plant material two ends libation at an ancient wedding ceremony dilution; Or after plant material two ends are sealed with preservative film, by bundled the quantity binding on demand of plant material.
In plants shoots incision film encapsulation method of the present invention, in step S1, filming agent wet weight is pivot weight 1000 times.
Implement filming agent preparation method of the present invention and plants shoots incision film encapsulation method, compared with the prior art, its beneficial effect is:
1. handling safety, convenient.The most key while sealing with wax is to grasp temperature, must be controlled at 80 DEG C~90 DEG C, and too high easy scald plant material is even scalded operating personnel; The too low wax that makes is dissolved not exclusively, causes plant material list mercolized wax bed thickness thin irregular.The technology of sealing with wax is because temperature control difficulty is large, and operation easier is high.Film encapsulation method of the present invention only need dip in branch material once in filming agent, simple and convenient.
2. there is disinfection function.The technology of sealing with wax is single branch itself to be carried out to Chinese wax sealing, can not be to the sterilizing of killing virus of branch otch.Film encapsulation method of the present invention is except sealing branch, also branch clip is carried out to sterilization treatment simultaneously, effectively reduce the soak evil of bacterium to branch otch, stopped branch and occurred because bacterium infects rotten situation, be specially adapted to the grafting membrane envelope of the plants such as the little seeds of a tung oil tree.
3. easy to use.Sealed with wax branch materials'use time to pass through paraffin removal processing, complicated operation.The branch material of film encapsulation method of the present invention processing does not need any processing, unpacks and can directly use, simple and convenient.
4. long fresh-keeping period.With tradition seal with wax process the comparison of branch freshness date, can extend storage life 10~20 days through film encapsulation method of the present invention branch material after treatment, meet the grafting requirement of long distance transportation.
5. applicability widely.Film encapsulation method of the present invention is except the little seeds of a tung oil tree, and other needs the plant materials such as each planting fruit-trees, flowers, green plants of long distance transportation, storage all can use;
6. easy to learn, easily promote.Film encapsulation method of the present invention can operate through the common laborer of general training, does not require that operator is professional and technical personnel, easily promotes.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
Comprise the steps: in filming agent preparation method of the present invention
The first step, dissolves nutrition composition: by appropriate corresponding dissolution with solvents for the nutrition composition of pivot weight suitable multiple.In the time that nutrition composition is water-soluble liquid, can directly use this nutrition composition.
Nutrition composition comprises indolebutyric acid, heteroauxin, indolebutyric acid potassium, brassin, the basic element of cell division, kinetin, CPPU etc., can adopt the one among them, also can adopt their combination.
Wherein, the solvent that indolebutyric acid, heteroauxin, indolebutyric acid potassium, brassin and the basic element of cell division are corresponding is that concentration is more than 80% ethanol, to the suitable multiple of pivot weight is: 2~10 times of indolebutyric acids, 5~30 times of heteroauxins, 2~10 times, indolebutyric acid potassium, 20~30 times of brassins, 1~2 times of the basic element of cell division; The solvent that kinetin is corresponding is glacial acetic acid; Kinetin is 3~4 times to the suitable multiple of pivot weight; The solvent that CPPU is corresponding is methyl alcohol; CPPU is 0.3~1 times to the suitable multiple of pivot weight.
Pivot weight can be set as any weight, and each component, taking this pivot weight as benchmark, is determined weight according to setting multiple.For example, setting pivot weight is 1 gram, 1 kilogram etc.
Second step, is added to the water the nutrition composition of the film-forming components of said reference weight suitable multiple and above-mentioned dissolving to stir, and obtains filming agent.
Film-forming components comprises the shitosan that mill taro powder, modified corn starch, concentration are 5%~10% etc., can adopt the one among them, also can adopt their combination.
Wherein, above-mentioned each film-forming components to the suitable multiple of pivot weight is: shitosan is appropriate, 50~100 times, mill taro powder, 50~100 times of modified corn starch.Amount ranges those skilled in the art of shitosan can determine by the test of limited number of time.
For improving the non-oxidizability of filming agent, in step 1, comprise dissolving antioxidant, by appropriate corresponding dissolution with solvents for the antioxidant of pivot weight suitable multiple.In step 2, the antioxidant of the nutrition composition dissolving in the film-forming components of pivot weight suitable multiple and step 1, dissolving is added to the water and is stirred, obtain filming agent.
Dissolve antioxidant and can use the ascorbic acid into d.The solvent that d ascorbic acid is corresponding is water, and d is anti-
_ bad hematic acid is 30~50 times to the suitable multiple of pivot weight.
In the various embodiments described above, in order to increase the sterilizing function of filming agent, in step 1, comprise dissolving bactericidal component: by appropriate corresponding dissolution with solvents for the bactericidal component of pivot weight suitable multiple; In step 2, comprise the bactericidal component of the nutrition composition of the film-forming components of pivot weight suitable multiple and above-mentioned dissolving and dissolving is added to the water and is stirred, obtain filming agent.
Bactericidal component comprises S-Ethyl ethylthio sulfonate, and the solvent that S-Ethyl ethylthio sulfonate is corresponding is that concentration is more than 80% ethanol, and S-Ethyl ethylthio sulfonate is 2~10 times to the suitable multiple of pivot weight.
In the various embodiments described above, in the time that step 1 adopts liquid fungicide, directly use the liquid fungicide of pivot weight suitable multiple; In step 2, comprise and add the liquid fungicide of step 1 to stir, obtain filming agent.
Liquid fungicide adopts and includes but not limited to little seeds of a tung oil tree based bacteriostat, and while adopting little seeds of a tung oil tree based bacteriostat, it is 2~10 times to the suitable multiple of pivot weight.
The above-mentioned filming agent making can need concentration according to use, is diluted with water to this concentration, obtains commercial filming agent, can directly use.Filming agent wet weight corresponding to normally used concentration range is pivot weight 500~1200 times.For example desirable filming agent wet weight is pivot weight 1000 times.Above-mentioned dilution step also can be carried out in the time using filming agent again.
For example, filming agent of the present invention can adopt following formula and step to be prepared:
Formula one
The first step, takes 2~10 grams of indolebutyric acids, 20~30 grams of brassins, adds appropriate 95% ethanol (concentration of alcohol can adopt more than 80% to absolute ethyl alcohol) to dissolve, and limit edged stirs; Take 3~4 grams of kinetins and add appropriate glacial acetic acid to dissolve, limit edged stirs; Take 0.3~1 gram of CPPU and add appropriate methyl alcohol to dissolve, limit edged stirs.
The shitosan that second step is 5%~10% by concentration mixes and is dissolved in water with the nutrition composition of step 1 in right amount, and limit edged is stirred to dissolving, obtains filming agent.This step can first add water and add afterwards shitosan.
In order to increase the sterilizing function of filming agent, in step 1, increase by 2~10 grams of S-Ethyl ethylthio sulfonates, add appropriate 95% ethanol (concentration of alcohol can adopt more than 80% to absolute ethyl alcohol) to dissolve, limit edged stirs; In step 2, the shitosan that is 5%~10% with appropriate concentration by the bactericidal component of the nutrition composition of dissolving and dissolving mixes, and is dissolved in water, and limit edged is stirred to abundant dissolving, obtains filming agent.
Formula two (establishing pivot weight is 1 gram):
The first step, takes 1~2 gram of 2~10 grams of S-Ethyl ethylthio sulfonate, 5~30 grams of heteroauxins, 25 grams of brassins, the basic element of cell division and adds appropriate 95% ethanol (can be more than 85% ethanol), and limit edged stirs, and makes it fully dissolve and mix; Take 30~50 grams, d_ ascorbic acid, smash powdered and be added to the water, limit edged is stirred to abundant dissolving.
Second step, takes 50~100 grams, mill taro powder and is added to the water, and limit edged is stirred to dissolving, mixes with first step component, adds 10 kg of water to mix, and obtains diluting the filming agent of 1000 times.
Formula three (establishing pivot weight is 1 gram):
The first step, takes 2~10 grams of S-Ethyl ethylthio sulfonates, 2~10 grams, indolebutyric acid potassium, 1~2 gram of the basic element of cell division, puts into appropriate 95% ethanol (can be more than 85% ethanol), stirs while feeding in raw material, and makes it fully dissolve and mix; Take 0.3~1 gram of CPPU and add in appropriate methyl alcohol, limit edged stirs, and makes it fully dissolve and mix.
Second step, takes 150~300 grams of Nafusakus, 50~100 grams of modified corn starch, mixes with first step component, adds 10 kg of water, and limit edged stirs, and makes it fully dissolve and mix, and obtains diluting the filming agent of 1000 times.
Plants shoots incision film encapsulation method of the present invention comprises the steps;
The first step, need put into container with the above-mentioned filming agent of weight and be diluted with water to and need concentration (for example diluting 1000 times), stir.Plant material is trimmed to and needs length and angle.
Second step, puts into respectively the filming agent libation at an ancient wedding ceremony that above-mentioned dilution is good once by the plant material two ends of having pruned, and the plant material two ends of the good filming agent of the libation at an ancient wedding ceremony are sealed with preservative film.
The 3rd step, ties up the plant material paper using suitcase dress of good seal.
In above-mentioned steps two, can be first by bundled the plant material of having pruned quantity binding on demand, then by the filming agent of plant material two ends libation at an ancient wedding ceremony dilution; Or after plant material two ends are sealed with preservative film, by bundled the quantity binding on demand of plant material.
In above-mentioned steps one, the filming agent wet weight of employing is pivot weight 1000 times.

Claims (10)

1. a filming agent preparation method, is characterized in that, comprises the steps:
A1, dissolving nutrition composition: by appropriate corresponding dissolution with solvents for the nutrition composition of pivot weight suitable multiple; Water-soluble liquid nutrition composition is directly used;
A2, the nutrition composition of the film-forming components of pivot weight suitable multiple and this dissolving is added to the water and is stirred, obtain filming agent.
2. filming agent preparation method as claimed in claim 1, it is characterized in that, described nutrition composition is indolebutyric acid, heteroauxin, indolebutyric acid potassium, brassin, the basic element of cell division, kinetin, CPPU one or a combination set of, wherein, the solvent that indolebutyric acid, heteroauxin, indolebutyric acid potassium, brassin and the basic element of cell division are corresponding is that concentration is more than 80% ethanol, to the suitable multiple of pivot weight is: 2~10 times of indolebutyric acids, 5~30 times of heteroauxins, 2~10 times, indolebutyric acid potassium, 20~30 times of brassins, 1~2 times of the basic element of cell division; The solvent that kinetin is corresponding is glacial acetic acid; Kinetin is 3~4 times to the suitable multiple of pivot weight; The solvent that CPPU is corresponding is methyl alcohol; CPPU is 0.3~1 times to the suitable multiple of pivot weight;
Described film-forming components is mill taro powder, modified corn starch, concentration shitosan that is 5%~10% one or a combination set of, wherein, to the suitable multiple of pivot weight is: shitosan is appropriate, 50~100 times, mill taro powder, 50~100 times of modified corn starch.
3. filming agent preparation method as claimed in claim 2, is characterized in that, filming agent wet weight is pivot weight 500~1200 times.
4. filming agent preparation method as claimed in claim 2, is characterized in that, steps A 1 comprises: dissolve antioxidant, by appropriate corresponding dissolution with solvents for the antioxidant of pivot weight suitable multiple; Steps A 2 comprises: add the antioxidant of the dissolving of steps A 1 to stir, obtain filming agent.
5. filming agent preparation method as claimed in claim 4, is characterized in that, described dissolving antioxidant is d ascorbic acid, and the solvent that d ascorbic acid is corresponding is water, and d ascorbic acid is 30~50 times to the suitable multiple of pivot weight.
6. the filming agent preparation method as described in one of claim 1 to 5, is characterized in that, described steps A 1 comprises dissolving bactericidal component: by appropriate corresponding dissolution with solvents for the bactericidal component of pivot weight suitable multiple; Described steps A 2 comprises: add the bactericidal component of the dissolving of steps A 1 to stir, obtain filming agent;
Or described steps A 1 comprises liquid fungicide, use the liquid fungicide of pivot weight suitable multiple; Described steps A 2 comprises: add the liquid fungicide of steps A 1 to stir, obtain filming agent.
7. filming agent preparation method as claimed in claim 6, is characterized in that, described bactericidal component is S-Ethyl ethylthio sulfonate, and the solvent that S-Ethyl ethylthio sulfonate is corresponding is ethanol, and S-Ethyl ethylthio sulfonate is 2~10 times to the suitable multiple of pivot weight;
Or described bactericidal component is little seeds of a tung oil tree based bacteriostat, little seeds of a tung oil tree based bacteriostat is 2~10 times to the suitable multiple of pivot weight.
8. a plants shoots incision film encapsulation method, is characterized in that, comprises the steps:
S1, need put into container with the filming agent of weight and be diluted with water to and need concentration, stir; Plant material is trimmed to and needs length and angle;
S2, above-mentioned dilution is put into respectively in the plant material two ends of having pruned the filming agent libation at an ancient wedding ceremony once, the plant material two ends of the good filming agent of the libation at an ancient wedding ceremony are sealed with preservative film;
S3, the plant material paper using suitcase dress of good seal is tied up.
9. plants shoots incision film encapsulation method as claimed in claim 8, is characterized in that, in step S2, first by bundled the plant material of having pruned quantity binding on demand, then by the filming agent of plant material two ends libation at an ancient wedding ceremony dilution; Or after plant material two ends are sealed with preservative film, by bundled the quantity binding on demand of plant material.
10. plants shoots incision film encapsulation method as claimed in claim 8 or 9, is characterized in that, in step S1, filming agent wet weight is pivot weight 1000 times.
CN201410131952.7A 2014-04-02 2014-04-02 Preparation method of jatropha curcas film coating agent Expired - Fee Related CN103931615B (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107889632A (en) * 2017-11-21 2018-04-10 恭城福茂生油茶文化产业发展有限公司 A kind of engrafting method of oil tea
CN109122672A (en) * 2018-07-18 2019-01-04 安徽德昌苗木有限公司 A kind of storage method of oil tea breeding fringe bar heat preserving antistaling
CN109392895A (en) * 2018-11-30 2019-03-01 云南省热带作物科学研究所 A kind of rubber tree budling store method suitable for transport
CN109805004A (en) * 2019-04-12 2019-05-28 上海应用技术大学 A kind of preservation method of Hipeastrum vittalum's Fresh Cutting flower

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US4374929A (en) * 1980-07-14 1983-02-22 Standard Oil Company (Indiana) Production of xanthan gum from a chemically defined medium introduction
CN101161075A (en) * 2007-11-27 2008-04-16 李开森 A protectant for fruit tree wound healing
CN102617224A (en) * 2012-03-07 2012-08-01 海南正业中农高科股份有限公司 Plant wound protective agent containing chitosan oligosaccharide
CN102771348A (en) * 2012-07-20 2012-11-14 中国农业科学院烟草研究所 Film coating agent for seedling tray and preparation and use method of film coating agent

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4374929A (en) * 1980-07-14 1983-02-22 Standard Oil Company (Indiana) Production of xanthan gum from a chemically defined medium introduction
CN101161075A (en) * 2007-11-27 2008-04-16 李开森 A protectant for fruit tree wound healing
CN102617224A (en) * 2012-03-07 2012-08-01 海南正业中农高科股份有限公司 Plant wound protective agent containing chitosan oligosaccharide
CN102771348A (en) * 2012-07-20 2012-11-14 中国农业科学院烟草研究所 Film coating agent for seedling tray and preparation and use method of film coating agent

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107889632A (en) * 2017-11-21 2018-04-10 恭城福茂生油茶文化产业发展有限公司 A kind of engrafting method of oil tea
CN109122672A (en) * 2018-07-18 2019-01-04 安徽德昌苗木有限公司 A kind of storage method of oil tea breeding fringe bar heat preserving antistaling
CN109392895A (en) * 2018-11-30 2019-03-01 云南省热带作物科学研究所 A kind of rubber tree budling store method suitable for transport
CN109805004A (en) * 2019-04-12 2019-05-28 上海应用技术大学 A kind of preservation method of Hipeastrum vittalum's Fresh Cutting flower

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