CN103931503A - Secondary utilization method of rooting medium for stevia rebaudiana - Google Patents
Secondary utilization method of rooting medium for stevia rebaudiana Download PDFInfo
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- CN103931503A CN103931503A CN201410199571.2A CN201410199571A CN103931503A CN 103931503 A CN103931503 A CN 103931503A CN 201410199571 A CN201410199571 A CN 201410199571A CN 103931503 A CN103931503 A CN 103931503A
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- stevia
- root
- seedling
- stevia rebaudiana
- medium
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Abstract
The invention discloses a secondary utilization method of a rooting medium for stevia rebaudiana. After tissue cultured rooted seedlings of stevia rebaudiana are cultured, the rooted seedlings are pulled out of a culture bottle, and single-node stems of stevia rebaudiana are reconnected to an original medium to be subjected to secondary rooting culture, so that residual nutrient substances in the medium can be fully used, and a crop of rooted seedlings can be re-obtained. The measure can save the tissue culture cost of stevia rebaudiana, thereby improving the economic benefits.
Description
Technical field
the present invention relates to stevia tissue culture technique field, say more specifically a kind of secondary utilization method of stevia root media.
Background technology
it is stevia fast breeding and the Important Platform that carries out a series of scientific researches that stevia tissue is cultivated.Stevia is after culture of rootage, the stem segment length of inoculation has become the various stevia of the root, stem and leaf seedling plant that takes root, for the multiple of expanding propagation, conventionally cover with after blake bottle at the branch of the seedling of taking root, it is extracted with root from medium, the stem section that again branch is cut into single-unit is carried out outdoor cuttage and seedling culture, and medium has been processed as refuse.But we analyze, and the medium in tissue culture bottle is through a culture of rootage, and nutriment wherein does not all exhaust, and can continue to be used.
Summary of the invention
the object of the invention is to cultivate the inadequate problem of nutrient utilization in the medium existing for the stevia seedling of taking root, propose a kind of secondary utilization method of stevia root media.
namely, after a culture of rootage of stevia completes, pull out the natural in medium, again access new stevia stem section and carry out culture of rootage, former root media is carried out to secondary utilization.
the present invention is achieved in that
a secondary utilization method for stevia root media, comprises the steps:
(1) extract seedling one time: the once group in stevia blake bottle is trained to the seedling of taking root and extract with root.
(2) access secondary seedling: access new stevia culture of rootage stem section in former root media.
(3) returning frame cultivates: tissue culture bottle is put back on culturing rack and continued to cultivate.
above-mentioned root media is 1/2MS medium.
beneficial effect of the present invention:
utilization of the present invention in the past will discarded medium re-starts the take root cultivation of seedling of stevia, remaining nutriment in former medium is utilized again, do not increasing under the prerequisite of material, recruitment, turn waste into wealth, one batch of seedling of taking root of many cultivations is for cottage propagation, produce and reduced consumption, the beneficial effect of additional income.
Embodiment
3 strain root media recycling examples of stevia
to B1 ,+3, No. 43 stevia strains are carried out culture of rootage, condition of culture: medium
1/2MS root media, 24 DEG C of temperature, illumination 2100lux/cm
2
, adopt 250ml blake bottle, 5 stevia list stem segments of every bottle graft kind, 10 bottles of each strains, cultivate 30 days.In the time that first batch of seedling of taking root cultivated 30 days, under superclean bench aseptic condition, from bottle, extract with root the seedling of taking root of first batch, then access 5 new stevia stem sections in residual media in each blake bottle, carry out regrowth hair and cultivate 30 days.While expiring, pulling out as method the seedling of taking root, inserting the stem section of the 3rd batch, cultivating 30 days.
investigation method:
each cultivation while expiring taken out the seedling of taking root, and counts total stem number of the every bottle of seedling of taking root, total joint number.Because the stevia seedling of taking root is mainly used for outdoor cuttage and seedling culture, cuttage is generally single-unit cuttage, and the top tip 1cm length of seedling is calculated a joint.So total stem number that culture of rootage obtains and total joint number number just become the index of proliferate efficiency height.The survey showed that for table 1: three strains are carried out secondary utilization to root media, all obtain obvious effect, the output of average total stem number is than once utilizing and increased by 69.9%, the output of average total joint number is than once utilizing and increased by 55%, and having little effect of utilizing for three times can be ignored.The above results explanation, the secondary utilization of stevia root media, has given full play to the nourishing effect of nutriment in former root media, has increased substantially the proliferate efficiency of stevia.
table 1. 3 strain root media recycling application forms of stevia
Claims (1)
1. a secondary utilization method for stevia root media, is characterized in that, it comprises the steps:
(1) extract seedling one time: the once group in stevia blake bottle is trained to the seedling of taking root and extract with root;
(2) access secondary seedling: in former root media, access new stevia culture of rootage stem section;
(3) returning frame cultivates: tissue culture bottle is put back on culturing rack and continued to cultivate;
Above-mentioned root media is 1/2MS medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410199571.2A CN103931503A (en) | 2014-05-13 | 2014-05-13 | Secondary utilization method of rooting medium for stevia rebaudiana |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410199571.2A CN103931503A (en) | 2014-05-13 | 2014-05-13 | Secondary utilization method of rooting medium for stevia rebaudiana |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103931503A true CN103931503A (en) | 2014-07-23 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410199571.2A Pending CN103931503A (en) | 2014-05-13 | 2014-05-13 | Secondary utilization method of rooting medium for stevia rebaudiana |
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| CN (1) | CN103931503A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106665348A (en) * | 2016-11-03 | 2017-05-17 | 明光市大全甜叶菊专业合作社 | Stevia rebaudiana rooting medium |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102165919A (en) * | 2011-01-27 | 2011-08-31 | 赣州菊隆高科技实业有限公司 | Tissue culture quick propagation technology of stevia rebaudiana seedlings |
| CN103766222A (en) * | 2014-02-12 | 2014-05-07 | 四川农业大学 | Stevia rebaudiana tissue culture method and culture medium thereof |
-
2014
- 2014-05-13 CN CN201410199571.2A patent/CN103931503A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102165919A (en) * | 2011-01-27 | 2011-08-31 | 赣州菊隆高科技实业有限公司 | Tissue culture quick propagation technology of stevia rebaudiana seedlings |
| CN103766222A (en) * | 2014-02-12 | 2014-05-07 | 四川农业大学 | Stevia rebaudiana tissue culture method and culture medium thereof |
Non-Patent Citations (3)
| Title |
|---|
| 姜长阳等: "研究性课程中发明创造技法的应用及创新能力培养探讨", 《实验研究与探索》 * |
| 崔广荣等: "甜叶菊的组织培养", 《安徽科技学院学报》 * |
| 郭达初等: "大花萱草组织培养快速繁殖", 《浙江农业学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106665348A (en) * | 2016-11-03 | 2017-05-17 | 明光市大全甜叶菊专业合作社 | Stevia rebaudiana rooting medium |
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Application publication date: 20140723 |