CN103926412A - Chip device for myocardial infarction diagnosis - Google Patents
Chip device for myocardial infarction diagnosis Download PDFInfo
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- CN103926412A CN103926412A CN201410166097.3A CN201410166097A CN103926412A CN 103926412 A CN103926412 A CN 103926412A CN 201410166097 A CN201410166097 A CN 201410166097A CN 103926412 A CN103926412 A CN 103926412A
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- main body
- sensor main
- area
- detection zone
- detection
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/32—Cardiovascular disorders
- G01N2800/324—Coronary artery diseases, e.g. angina pectoris, myocardial infarction
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Dispersion Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses a chip device for myocardial infarction diagnosis. The chip device is characterized by comprising a sensor main body, wherein a sample injection area, a columnar filtering area, a reagent storage area, a detection area, a micro channel, a magnetic device and an optical detection device are sequentially arranged on the sensor main body along a liquid flow direction; the sample injection area is used for dropwise adding a whole blood sample; the columnar filtering area is used for filtering blood cells and only allows proteins in blood to pass; reagents for a biochemical reaction are arranged in the reagent storage area and comprise a magnetic bead labeled anti-human cTnI monoclonal antibody and a luminescence molecule labeled anti-human cTnI monoclonal antibody; the reagent storage area and the detection area are communicated through the micro channel; the magnetic device is arranged below the sensor main body and is positioned just under the detection area; the optical detection device is arranged above the sensor main body and is positioned just above the detection area. According to the device, quantitative and accurate detection can be realized, the detection time is short, and the device is small in size and portable.
Description
Technical field
The present invention relates to a kind of technical field that detects cardiac muscle troponin I, the chip apparatus that especially heart stalk protein marker detects.
Background technology
Troponin is made up of Troponin I, T, tri-kinds of subunits of C, in contraction of muscle and diastole process, plays important regulative.When after myocardial damage, cardiac troponin compound is discharged in blood, and in three kinds of subunits, Troponin I has Cardiac-specific and the sensitivity of height, so cardiac muscle troponin I has become current optimal myocardial infarction mark.
Cardiac muscle troponin I conventional sense method has radioimmunoassay method (RIAs), high pressure liquid chromatographic analysis method, electrochemical methods, protein analyzer, various diagnostic kit and immunochromatography biochip etc., there is radioactive contamination, need large-scale instrument in these methods, length consuming time, the shortcoming such as can not quantitatively detect, application clinically has certain limitation, is difficult for promoting the use of.
Summary of the invention
The shortcoming existing in order to overcome above-mentioned prior art, the object of the present invention is to provide a kind of chip apparatus for the diagnosis of heart stalk, and this device can be realized quantitative detection, and volume is little is again easy to carry, and detection speed is also fast.
In order to address the above problem, the present invention by the following technical solutions: a kind of chip apparatus for heart stalk diagnosis, it is characterized in that, comprise sensor main body, on described sensor main body, be disposed with along liquid flow path direction:
A sample application zone, for dripping whole blood sample;
A column filtrating area, for the haemocyte of filtering blood, only allows the protein in blood flow through;
A reagent storage area, arranges biochemical reaction reagent used in this district; Described pack is containing the anti-human cTnI monoclonal antibody of a strain marked by magnetic bead, the anti-human cTnI monoclonal antibody of a strain light emitting molecule mark;
A detection zone,
A micro-raceway groove, is communicated with described reagent storage area with described detection zone;
A magnetic means, be arranged on described sensor main body below and be positioned at described detection zone under;
An optical detection apparatus, be arranged on described sensor main body top and be positioned at described detection zone directly over.
Further: along liquid flow path direction, be provided with waste liquid district in the front of detection zone, and described detection zone to be connected with described waste liquid district by raceway groove.
Further: described sensor main body adopts glass to make.
Further: described sensor main body adopts PDMS material to make.
The invention has the beneficial effects as follows: compared with it detects cTnI with the large-scale Biochemical Analyzer of tradition, its detection time is short, detecting instrument miniature portable; Can realize quantitatively and accurately detecting, and method for quick is all generally qualitative detection at present.
Brief description of the drawings
Below in conjunction with drawings and Examples, the present invention is described further:
Fig. 1 is the structure of the embodiment of the present invention and uses view;
Fig. 2 is the structural representation of column filtrating area in the embodiment of the present invention;
Fig. 3 is the graph of a relation of cTnI content and luminous intensity in blood sample;
In figure: 1 sample application zone, 2 reagent storage areas, 3 micro-raceway grooves, 4 detection zones, 5 column filtrating areas, 51 Filter columns, 6 magnetic means, 7 optical detection apparatuss, 8 whole blood samples, 9 waste liquid districts, 10 sensor main bodies, 11 haemocytes, 12 protein.
Embodiment
As shown in Figure 1, the chip apparatus that this is used for the diagnosis of heart stalk, comprises sensor main body 10, on described sensor main body 10, is disposed with along liquid flow path direction:
A sample application zone 1, for dripping whole blood sample 8;
A column filtrating area 5, for the haemocyte of filtering blood, only allows the protein in blood flow through;
A reagent storage area 2, arranges biochemical reaction reagent used in this district; Described pack is containing the anti-human cTnI monoclonal antibody of a strain marked by magnetic bead, the anti-human cTnI monoclonal antibody of a strain light emitting molecule mark;
A detection zone 4;
A micro-raceway groove 3, is communicated with described reagent storage area 2 with described detection zone 4;
A magnetic means 6, be arranged on described sensor main body 10 belows and be positioned at described detection zone 4 under;
An optical detection apparatus 7, be arranged on described sensor main body 10 tops and be positioned at described detection zone 4 directly over.
Further: along liquid flow path direction, be provided with waste liquid district 9 in the front of detection zone 4, and described detection zone 4 to be connected with described waste liquid district 9 by raceway groove.
Further: described sensor main body 10 adopts glass to make; Also can adopt the materials such as PDMS to make.
Utilize the detecting step of said chip device detection heart stalk marker protein as follows:
A), get 100 microlitre whole bloods (or serum), be added drop-wise to the sample application zone 1 of biochip, utilize several Filter columns 51 of column filtrating area 5 that haemocyte 11 is filtered, as shown in Figure 2, only allow the serum that contains range protein 12 to pass through;
B), serum flows to reagent storage area 2, the cTnI in serum, as antigen, forms double-antibody sandwich compound with the anti-human cTnI monoclonal antibody of marked by magnetic bead and the anti-human cTnI monoclonal antibody generation immune response of light emitting molecule mark respectively;
C), this compound continues to flow along micro-raceway groove 3 and arrives behind detection zone 4, the magnetic means 6 of 4 belows, detected district attracts and is trapped in detection zone 4, unnecessary reagent continues to flow to waste liquid district 9;
D), in detection zone 4, add luminescent indicator, the optical detection apparatus that is arranged in 4 tops, detection zone detects this and becomes the light signal of correlativity with the amount of blood cTnI to be measured, thereby realizes the quantitative detection to cTnI.In blood sample, the relation of cTnI content and luminous intensity as shown in Figure 3.
Claims (4)
1. for a chip apparatus for heart stalk diagnosis, it is characterized in that, comprise sensor main body, on described sensor main body, be disposed with along liquid flow path direction:
A sample application zone, for dripping whole blood sample;
A column filtrating area, for the haemocyte of filtering blood, only allows the protein in blood flow through;
A reagent storage area, arranges biochemical reaction reagent used in this district; Described pack is containing the anti-human cTnI monoclonal antibody of a strain marked by magnetic bead, the anti-human cTnI monoclonal antibody of a strain light emitting molecule mark;
A detection zone,
A micro-raceway groove, is communicated with described reagent storage area with described detection zone;
A magnetic means, be arranged on described sensor main body below and be positioned at described detection zone under;
An optical detection apparatus, be arranged on described sensor main body top and be positioned at described detection zone directly over.
2. a kind of chip apparatus for the diagnosis of heart stalk according to claim 1, is characterized in that: along liquid flow path direction, be provided with waste liquid district in the front of detection zone, and described detection zone is connected with described waste liquid district by raceway groove.
3. a kind of chip apparatus for the diagnosis of heart stalk according to claim 1 and 2, is characterized in that: described sensor main body adopts glass to make.
4. a kind of chip apparatus for the diagnosis of heart stalk according to claim 1 and 2, is characterized in that: described sensor main body adopts PDMS material to make.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410166097.3A CN103926412A (en) | 2014-04-24 | 2014-04-24 | Chip device for myocardial infarction diagnosis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410166097.3A CN103926412A (en) | 2014-04-24 | 2014-04-24 | Chip device for myocardial infarction diagnosis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103926412A true CN103926412A (en) | 2014-07-16 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410166097.3A Pending CN103926412A (en) | 2014-04-24 | 2014-04-24 | Chip device for myocardial infarction diagnosis |
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| CN (1) | CN103926412A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1854737A (en) * | 2005-04-28 | 2006-11-01 | 穆海东 | Multi-index protein chip inspection reagent unit of cardiovascular disease diagnosis and prediction |
| CN201707336U (en) * | 2010-05-11 | 2011-01-12 | 山东大学 | Test strip device for fast and quantitatively detecting first cardiac troponin |
| CN102305866A (en) * | 2011-05-20 | 2012-01-04 | 山东大学 | Detection device for quickly diagnosing acute myocardial infarction |
| WO2012019108A1 (en) * | 2010-08-05 | 2012-02-09 | Abbott Point Of Care Inc. | Magnetic immunosensor with trench configuration and method of use |
| JP2013145138A (en) * | 2012-01-13 | 2013-07-25 | Konica Minolta Inc | Immunoassay of troponin using spfs (surface plasmon field-enhanced fluorescence spectroscopy) |
-
2014
- 2014-04-24 CN CN201410166097.3A patent/CN103926412A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1854737A (en) * | 2005-04-28 | 2006-11-01 | 穆海东 | Multi-index protein chip inspection reagent unit of cardiovascular disease diagnosis and prediction |
| CN201707336U (en) * | 2010-05-11 | 2011-01-12 | 山东大学 | Test strip device for fast and quantitatively detecting first cardiac troponin |
| WO2012019108A1 (en) * | 2010-08-05 | 2012-02-09 | Abbott Point Of Care Inc. | Magnetic immunosensor with trench configuration and method of use |
| CN102305866A (en) * | 2011-05-20 | 2012-01-04 | 山东大学 | Detection device for quickly diagnosing acute myocardial infarction |
| JP2013145138A (en) * | 2012-01-13 | 2013-07-25 | Konica Minolta Inc | Immunoassay of troponin using spfs (surface plasmon field-enhanced fluorescence spectroscopy) |
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Application publication date: 20140716 |