CN103918896A - Preparation method for producing anti-mycotoxin feed additive by fermenting plants with lactobacillus - Google Patents
Preparation method for producing anti-mycotoxin feed additive by fermenting plants with lactobacillus Download PDFInfo
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- CN103918896A CN103918896A CN201410140004.XA CN201410140004A CN103918896A CN 103918896 A CN103918896 A CN 103918896A CN 201410140004 A CN201410140004 A CN 201410140004A CN 103918896 A CN103918896 A CN 103918896A
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Abstract
The invention relates to a preparation method for producing anti-mycotoxin feed additive by fermenting plants with lactobacillus. Many researches indicate that high-concentration mycotoxin can cause acute poisoning of animals, and the animals can have the symptom of chronic poisoning even if fed with the feed containing low-concentration mycotoxin. The hazards of the mycotoxin are mainly expressed in destroying and lowering nutrients in the raw material or feed, causing viscera and tissue damage of animals and causing severe breeding difficulty, immunosuppression and the like; and such influences can do harm to the human health through the food chains of meat, eggs, milk and the like. The method comprises the following steps: (1) primary strain preparation; (2) secondary strain preparation; (3) lactobacillus solution culture; and (4) plant fermentation. The method is for producing the anti-mycotoxin feed additive by fermenting plants with lactobacillus.
Description
technical field:
the present invention relates to a kind of preparation method of lactobacillus-fermented plant production antifungi toxin feed addictive.
background technology:
in feed, multiple mycotoxin coexists, and that mycotoxin harm is maximum, the condition when depending on grain harvest and storing, and solving mycotoxins in feed pollution is a worldwide problem, is the large problem that solves food security and human health.China south China, East China and even North China's harvest season rainfall are more, and crops will be subject to the pollution of mould in field.Particularly aflatoxin is extensively present in the feedstuff such as corn, peanut meal, and the production performance of serious threat animal causes huge economic loss to every year feed industry and animal husbandry.Estimate according to FAO (Food and Agriculture Organization of the United Nation) (FAO), 25% of the annual grain yield in the whole world is subject to mycotoxin contamination.Pittet (1998) has summed up the investigation result of mycotoxin in 27 food and feed samples all over the world, find that vomitoxin and FT are the most general mycotoxins, in grain sample, the recall rate of vomitoxin is 75%, the mean concentration of each sample is 62.1mg/kg, the recall rate of FT is 66%, the mean concentration of each sample is 37.6mg/kg, and the recall rate of Gibberella zeae toxin, AFB1 is all below 50%.Have report to show aflatoxin, T-2 toxin, DON, ZEA, OTA, FT ubiquity in feed and feedstuff, and the recall rate of mycotoxin will be higher than single energy feed in mixed feed.It is pointed out that evaluating mould depends primarily on mould content of molds in feed to the pollution level of feed, instead of the recall rate of mould, and evaluate mould to the harm of livestock and poultry depend on toxigenic bacterium strain and content of toxins how many.Mycotoxin is a global problem to the pollution of feed and raw material, and the whole world approximately has 5% ~ 7% grain and feed to occur to go mouldy every year according to statistics, has caused tremendous economic loss.Numerous research shows, the mycotoxin of high concentration can make animal acute poisoning, also can make animal generation slow poisoning symptom even if feed containing the feed of low concentration mycotoxin.The harm of mycotoxin is mainly manifested in the nutrient that can destroy and reduce in raw material or feed, causes animal viscera and tissue damage, causes serious breeding difficulty and immunosupress etc., and these impacts can also be passed through the food chains such as meat, egg and milk human health is produced to harm.
summary of the invention:
the object of this invention is to provide a kind of preparation method of lactobacillus-fermented plant production antifungi toxin feed addictive.
above-mentioned object realizes by following technical scheme:
a preparation method for lactobacillus-fermented plant production antifungi toxin feed addictive, the method comprises the steps:
(1) preparation of first class inoculum;
(2) preparation of second class inoculum;
(3) lactobacillus suspension is cultivated;
(4) fermenting plant.
the preparation method of described lactobacillus-fermented plant production antifungi toxin feed addictive, the method of the preparation of the first class inoculum described in step (1) is by the soup dilution of lactic acid bacteria culturers ordinary lactic acid bacteria culture medium or after the breeding of ordinary lactic acid bacteria culture medium soup, be inoculated in lactic acid bacteria culture medium flat plate, cultivate 22--24h for 37 DEG C, choose bacterium colony for circular, median size, projection, micro white, moistening, neat in edge median size bacterium colony 5-10, under aseptic condition, mix and be inoculated in respectively some of lactic acid bacteria agar slants, cultivate 24h for 37 DEG C, through being purely up to the standards, as first class inoculum, 2-8 DEG C of preservation, should be no more than 2 months, portable 1-2 time around here, subculture on culture medium, be no more than for 4 generations.
the preparation method of described lactobacillus-fermented plant production antifungi toxin feed addictive, described lactic acid bacteria culture medium flat plate, ordinary lactic acid bacteria agar slant consist of: sucrose 3%, agar 1.5%, peptone 0.5%, yeast extract 0.5%, KH
2
pO
4
0.1%, the Tween80 aqueous solution 0.5%, CaCO
3
2%.
the preparation method of described lactobacillus-fermented plant production antifungi toxin feed addictive, the preparation method of the second class inoculum described in step (2) gets the flat bottle of first class inoculum inoculating lactic acid bacterium soup agar, cultivate after 24h for 37 DEG C, rinse with lactic acid bacteria culture solution that to access loading amount after lawn be in 10,000 bottles of 9000ml, 37 DEG C of cultivations, after passed examination, as secondary seed.2-8 DEG C of preservation, should be no more than 3.
the preparation method of described lactobacillus-fermented plant production antifungi toxin feed addictive, the flat bottle of described lactic acid bacteria soup agar consists of: peptone 10.0g meat extract 10.0g dusty yeast 5.0g K
2
hPO
4
2.0g diammonium hydrogen citrate 2.0g anhydrous sodium acetate 5.0g glucose 20.0g Tween-80 1.0ml MgSO
4
0.28g MnSO
4
h
2
o 0.2g distilled water 1.0L pH6.8.
the preparation method of described lactobacillus-fermented plant production antifungi toxin feed addictive, the described lactobacillus suspension cultural method of step (3) is that second class inoculum is inoculated into first 38 DEG C of cultivation 14h of the static cultivation of alternating temperature anaerobism in lactic acid bacteria culture solution according to 5% inoculum concentration, cool to again 30 DEG C and cultivate 20h, 2-8 DEG C of preservation, should be no more than 3.
the preparation method of described lactobacillus-fermented plant production antifungi toxin feed addictive, the described fermenting plant method of step (4) be lactobacillus suspension three-class strain after the assay was approved, lactobacillus suspension is brought Selection In and according to mixing in the composite plant powder preparing, make lactobacillus suspension content reach 5% and 5% lactic acid bacteria soup culture medium, fully mix, in 30--38 DEG C of environment, carry out anaerobic fermentation, adopt first 38 DEG C of high temperature anaerobic glycolysis 48 hours, 30 DEG C of low-temp anaerobic fermentations 72 hours again, 38 DEG C of hot fermentations 48 hours again, then 30 DEG C of cold fermentations 192 hours were both.
lactobacillus-fermented plant production antifungi toxin feed addictive prepared by the method for one of above-mentioned method.
beneficial effect:
1. the present invention is the feed addictive by the plant production of lactobacillus-fermented antifungi toxin, be actually a kind of antifungi toxin product of composite parts, lactic acid bacteria in this product plays suction-operated to part mycotoxin, in process of production, lactic acid bacteria glycolysis plant, can destroy the cell membrane of plant, effective ingredients in plant is fully discharged, the mycotoxin in feed is played to better suction-operated and complexing.
2. the present invention overcomes the unicity of existing common antifungi toxin feed addictive effect, virus elimination rate is low, the suction-operated of single dependence mineral matter element, or detoxifying agent itself is chemical composition, in detoxification, affect absorbing or thinking chemical composition is taken to feed of nutriment, cause the dangerous of feed, remote-effects animal foods dangerous, this method provides a kind of production method of new antifungi toxin feed addictive, a large amount of lactic acid that this production method can make lactic acid bacteria breeding produce, malic acid, citric acid, palmitic acid and other compositions decompose the cell membrane of natural plant composition, active ingredient in plant cell wall is fully discharged, so that better absorption, the mycotoxin composition of the different cultivars in complexing feed, the product of producing like this, mycotoxin in lactic acid bacteria and the multipair feed of multiple natural plants has degradation, be a joint product, also be a kind of natural, high efficiency products, reduce the adding proportion in feed, also multiple mycotoxin is played to degradation.
detailed description of the invention:
embodiment 1:
a preparation method for lactobacillus-fermented plant production antifungi toxin feed addictive, the method comprises the steps:
(1) preparation of first class inoculum;
(2) preparation of second class inoculum;
(3) lactobacillus suspension is cultivated;
(4) fermenting plant;
embodiment 2:
according to the preparation method of the lactobacillus-fermented plant production antifungi toxin feed addictive described in embodiment 1, the method of the preparation of the first class inoculum described in step (1) is by the soup dilution of lactic acid bacteria culturers ordinary lactic acid bacteria culture medium or after the breeding of ordinary lactic acid bacteria culture medium soup, be inoculated in lactic acid bacteria culture medium flat plate, cultivate 22--24h for 37 DEG C, choose bacterium colony for circular, median size, projection, micro white, moistening, neat in edge median size bacterium colony 5-10, under aseptic condition, mix and be inoculated in respectively some of lactic acid bacteria agar slants, cultivate 24h for 37 DEG C, through being purely up to the standards, as first class inoculum, 2-8 DEG C of preservation, should be no more than 2 months, portable 1-2 time around here, subculture on culture medium, be no more than for 4 generations.
embodiment 3:
according to the preparation method of the lactobacillus-fermented plant production antifungi toxin feed addictive described in embodiment 1 or 2, described lactic acid bacteria culture medium flat plate, ordinary lactic acid bacteria agar slant consist of: sucrose 3%, agar 1.5%, peptone 0.5%, yeast extract 0.5%, KH
2
pO
4
0.1%, the Tween80 aqueous solution 0.5%, CaCO
3
2%.
embodiment 4:
preparation method according to the lactobacillus-fermented plant production antifungi toxin feed addictive described in embodiment 1 or 2 or 3: the preparation method of the second class inoculum described in step (2) gets the flat bottle of first class inoculum inoculating lactic acid bacterium soup agar, cultivate after 24h for 37 DEG C, rinse with lactic acid bacteria culture solution that to access loading amount after lawn be in 10,000 bottles of 9000ml, 37 DEG C of cultivations, after passed examination, as secondary seed.2-8 DEG C of preservation, should be no more than 3.
embodiment 5:
according to the preparation method of the lactobacillus-fermented plant production antifungi toxin feed addictive described in embodiment 1 or 2 or 3 or 4, the flat bottle of described lactic acid bacteria soup agar consists of: peptone 10.0g meat extract 10.0g dusty yeast 5.0g K
2
hPO
4
2.0g diammonium hydrogen citrate 2.0g anhydrous sodium acetate 5.0g glucose 20.0g Tween-80 1.0ml MgSO
4
0.28g MnSO
4
h
2
o 0.2g distilled water 1.0L pH6.8.
embodiment 6:
according to the preparation method of the lactobacillus-fermented plant production antifungi toxin feed addictive described in embodiment 1 or 2 or 3 or 4 or 5, the described lactobacillus suspension cultural method of step (3) is that second class inoculum is inoculated into first 38 DEG C of cultivation 14h of the static cultivation of alternating temperature anaerobism in lactic acid bacteria culture solution according to 5% inoculum concentration, cool to again 30 DEG C and cultivate 20h, 2-8 DEG C of preservation, should be no more than 3.
embodiment 7:
according to the preparation method of the lactobacillus-fermented plant production antifungi toxin feed addictive described in embodiment 1 or 2 or 3 or 4 or 5 or 6, the described fermenting plant method of step (4) be lactobacillus suspension three-class strain after the assay was approved, lactobacillus suspension is brought Selection In and according to mixing in the composite plant powder preparing, make lactobacillus suspension content reach 5% and 5% lactic acid bacteria soup culture medium, fully mix, in 30--38 DEG C of environment, carry out anaerobic fermentation, adopt first 38 DEG C of high temperature anaerobic glycolysis 48 hours, 30 DEG C of low-temp anaerobic fermentations 72 hours again, 38 DEG C of hot fermentations 48 hours again, 30 DEG C of cold fermentations had both obtained for 192 hours again.
embodiment 8;
lactobacillus-fermented plant production antifungi toxin feed addictive prepared by the method for one of the above embodiments 1-7.
Claims (8)
1. a preparation method for lactobacillus-fermented plant production antifungi toxin feed addictive, is characterized in that: the method comprises the steps:
(1) preparation of first class inoculum;
(2) preparation of second class inoculum;
(3) lactobacillus suspension is cultivated;
(4) fermenting plant;
The preparation method of lactobacillus-fermented plant production antifungi toxin feed addictive according to claim 1, it is characterized in that: the method for the preparation of the first class inoculum described in step (1) is by the soup dilution of lactic acid bacteria culturers ordinary lactic acid bacteria culture medium or after the breeding of ordinary lactic acid bacteria culture medium soup, be inoculated in lactic acid bacteria culture medium flat plate, cultivate 22--24h for 37 DEG C, choose bacterium colony for circular, median size, projection, micro white, moistening, neat in edge median size bacterium colony 5-10, under aseptic condition, mix and be inoculated in respectively lactic acid bacteria agar slant, cultivate 24h for 37 DEG C, through being purely up to the standards, as first class inoculum, 2-8 DEG C of preservation, should be no more than 2 months, portable 1-2 time around here, subculture on culture medium, be no more than for 4 generations.
2. the preparation method of lactobacillus-fermented plant production antifungi toxin feed addictive according to claim 2, it is characterized in that: described lactic acid bacteria culture medium flat plate, ordinary lactic acid bacteria agar slant consist of: sucrose 3%, agar 1.5%, peptone 0.5%, yeast extract 0.5%, KH
2pO
40.1%, the Tween80 aqueous solution 0.5%, CaCO
32%.
3. according to the preparation method of the lactobacillus-fermented plant production antifungi toxin feed addictive described in claim 1 or 2 or 3, it is characterized in that: the preparation method of the second class inoculum described in step (2) gets the flat bottle of first class inoculum inoculating lactic acid bacterium soup agar, cultivate after 24h for 37 DEG C, rinse with lactic acid bacteria culture solution that to access loading amount after lawn be in 10,000 bottles of 9000ml, 37 DEG C of cultivations, after passed examination, as secondary seed.
4. 2-8 DEG C of preservation, should be no more than 3.
5. according to the preparation method of the lactobacillus-fermented plant production antifungi toxin feed addictive described in claim 1 or 2 or 3 or 4, it is characterized in that: the flat bottle of described lactic acid bacteria soup agar consists of: peptone 10.0g meat extract 10.0g dusty yeast 5.0g K
2hPO
42.0g diammonium hydrogen citrate 2.0g anhydrous sodium acetate 5.0g glucose 20.0g Tween-80 1.0ml MgSO
40.28g MnSO
4h
2o 0.2g distilled water 1.0L pH6.8.
6. according to the preparation method of the lactobacillus-fermented plant production antifungi toxin feed addictive described in claim 1 or 2 or 3 or 4 or 5, it is characterized in that: the described lactobacillus suspension cultural method of step (3) is that second class inoculum is inoculated into first 38 DEG C of cultivation 14h of the static cultivation of alternating temperature anaerobism in lactic acid bacteria culture solution according to 5% inoculum concentration, cool to again 30 DEG C and cultivate 20h, 2-8 DEG C of preservation, should be no more than 3.
7. according to the preparation method of the lactobacillus-fermented plant production antifungi toxin feed addictive described in claim 1 or 2 or 3 or 4 or 5 or 6, it is characterized in that: the described fermenting plant method of step (4) be lactobacillus suspension three-class strain after the assay was approved, lactobacillus suspension is brought Selection In and according to mixing in the composite plant powder preparing, make lactobacillus suspension content reach 5% and 5% lactic acid bacteria soup culture medium, fully mix, in 30--38 DEG C of environment, carry out anaerobic fermentation, adopt first 38 DEG C of high temperature anaerobic glycolysis 48 hours, 30 DEG C of low-temp anaerobic fermentations 72 hours again, 38 DEG C of hot fermentations 48 hours again, 30 DEG C of cold fermentations had both obtained for 192 hours again.
8. the lactobacillus-fermented plant production antifungi toxin feed addictive that prepared by the method for one of claim 1 or 2 or 3 or 4 or 5 or 6 or 7.
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| Application Number | Priority Date | Filing Date | Title |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105230966A (en) * | 2015-09-30 | 2016-01-13 | 河南科技大学 | Fermented feed for animals, and preparation method thereof |
| CN113966788A (en) * | 2021-11-03 | 2022-01-25 | 武汉轻工大学 | Composition for relieving pig vomitoxin poisoning, feed additive, feed and preparation method |
-
2014
- 2014-04-09 CN CN201410140004.XA patent/CN103918896A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 崔彦召等: "不同乳酸菌剂对发酵全混合日粮霉菌毒素含量的影响", 《上海交通大学学报（农业科学版）》 * |
| 龙淼等: "乳酸菌清除霉菌毒素作用及其机制", 《饲料工业》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105230966A (en) * | 2015-09-30 | 2016-01-13 | 河南科技大学 | Fermented feed for animals, and preparation method thereof |
| CN113966788A (en) * | 2021-11-03 | 2022-01-25 | 武汉轻工大学 | Composition for relieving pig vomitoxin poisoning, feed additive, feed and preparation method |
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Application publication date: 20140716 |
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