CN103918894A - Ruminant feed added with aerosol OT, and feeding method thereof - Google Patents
Ruminant feed added with aerosol OT, and feeding method thereof Download PDFInfo
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- CN103918894A CN103918894A CN201410101709.0A CN201410101709A CN103918894A CN 103918894 A CN103918894 A CN 103918894A CN 201410101709 A CN201410101709 A CN 201410101709A CN 103918894 A CN103918894 A CN 103918894A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
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- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The present invention relates to a ruminant feed added with an aerosol OT, and a feeding method thereof. The feed comprises 0.04-0.12 part by weight of an aerosol OT, preferably 0.04-0.1 part by weight of the aerosol OT, more preferably 0.04-0.08 part by weight of the aerosol OT. With application of the feed of the present invention, the free feed intake of the ruminant is increased, the number of the rumen protozoa is reduced while the number of the rumen bacteria is increased, amino acids and other components reaching small intestine are significantly increased, amino acids and other nutrients digested and absorbed by small intestine are significantly increased, and the slaughter performance of the ruminant can be substantially improved.
Description
Technical field
The present invention relates to animal feeding field, be specifically related to a kind of ruminant feed and method for breeding thereof of feeding dioctylis sulfosuccinas natricus that add.
Background technology
The feature of ruminant digestion is to have ruminal digestion function.From zootrophic angle, ruminal digestion has following features: (1) is digest cellulose class material in large quantities, produce acetic acid etc., and the nonruminants such as chicken pig are very low to the digestion power of cellulose substances, and digestion process mainly occurs in large intestine; (2) starchy material is had to very strong fermentability, tunning comprises propionic acid etc., and the nonruminants such as chicken pig mainly occur in small intestine and stomach to the digestion of starch, and main end-product is glucose; (3) forage protein is degraded in large quantities in cud, and main end-product is ammonia, and the nonruminants such as chicken, pig mainly occur in stomach and small intestine to the digestion of forage protein, and main end-product is amino acid; (4) microprotein producing in cud is the important proteinaceous nutrient source of ruminant, and at nonruminant, the habit except rabbit with the soft excrement of food can be utilized the microprotein producing in alimentary canal, generally cannot utilize the microprotein producing in alimentary canal; (5) nonprotein nitrogen, as urea nitrogen etc., is converted into microprotein thereby can directly be utilized by microorganism in cud, and then as the source of animal protein nutrition, and nonruminant does not generally have the ability of utilizing nonprotein nitrogen.
In cud, do not have glandula digestive not secrete digestive enzymes yet. the essence of ruminal digestion is microorganism digestion.Rumen microflora is mainly by Anaerobic Bacteria, protozoon and anaerobic fungi three major types the composition of the microorganism, and the enzyme that they produce is maintaining the digestion process of cud, and microorganism itself provides various nutriments to animal again simultaneously.
The regulation and control that ruminal digestion metabolic process or intensity and rumen microflora form are one of emphasis of studying of people always, its object be to improve feed digestibility or usability, improve the nutrition condition of animal, reduce production costs, improve productivity and the product quality of animal.The regulation and control content of digestion and metabolism comprises reduction high-quality feed proteolytic rate, raising nonprotein nitrogen utilization rate, the microprotein output that improves, raising roughage digestibility, the activity that improves cellulose enzyme, reduction amylofermentation degree, the generation of minimizing methane etc.The regulation and control of rumen microflora comprise quantity, increase fungi, minimizing or the expeling cud protozoon etc. that increase and decrease each bacterioid.
Dioctylis sulfosuccinas natricus (Aerosol OT), chemical name is dioctyl sodium sulfosuccinate (Sodium dioctyl sulfosuccinate), it is being pharmaceutically non-irritating caccagogue, be usually used in defecation inability or postoperative patient, in ruminant animal nutrition research, for dispelling cud protozoon.
There is no at present the report for ruminant feed by dioctylis sulfosuccinas natricus.
Summary of the invention
An object of the present invention is to provide the feed of a kind of ruminant, this feed link is crossed and as a kind of new adding, is fed composition using dioctylis sulfosuccinas natricus, to increase searching for food and digestion power of ruminating, thereby improves production performance.Another object of the present invention is to provide the method for breeding of a kind of ruminant.Another object of the present invention is to provide a kind of application of above-mentioned ruminant feed.
Above-mentioned purpose of the present invention is achieved through the following technical solutions.
On the one hand, the invention provides a kind of ruminant feed of feeding dioctylis sulfosuccinas natricus that adds, by parts by weight of raw materials, described feed comprises 0.04 part to 0.12 part of dioctylis sulfosuccinas natricus, and preferably, described feed comprises 0.04 part to 0.1 part of dioctylis sulfosuccinas natricus; More preferably, described feed comprises 0.04 part to 0.08 part of dioctylis sulfosuccinas natricus.
Preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.12 part of 4 parts to 5 parts of maize straws, 58 parts to 65 parts of corn flour, 6.5 parts to 8.5 parts of soy bean powders, 20 parts to 24 parts of Cottonseed Meals, 2.5 parts to 3.5 parts of stone flours, 0.2 part to 1.3 parts of nutritive additive and dioctylis sulfosuccinas natricus; Preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.1 part of 4 parts to 5 parts of maize straws, 58 parts to 65 parts of corn flour, 6.5 parts to 8.5 parts of soy bean powders, 20 parts to 24 parts of Cottonseed Meals, 2.5 parts to 3.5 parts of stone flours, 0.2 part to 1.3 parts of nutritive additive and dioctylis sulfosuccinas natricus; More preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.08 part of 4 parts to 5 parts of maize straws, 58 parts to 65 parts of corn flour, 6.5 parts to 8.5 parts of soy bean powders, 20 parts to 24 parts of Cottonseed Meals, 2.5 parts to 3.5 parts of stone flours, 0.2 part to 1.3 parts of nutritive additive and dioctylis sulfosuccinas natricus.The feed of this composition can be the ruminant feed of 40 age in days to 80 ages in days.Above material nutrient component scope: organic matter >=93.0%, crude protein >=18.0%, lysine >=1.0%, cellulose≤5.0%, calcium >=1.0%, phosphorus >=0.5%.
Preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.12 part of 10 parts to 18 parts of maize straws, 55 parts to 65 parts of corn flour, 4.5 parts to 6.5 parts of soy bean powders, 15 parts to 25 parts of Cottonseed Meals, 1.0 parts to 2.5 parts of stone flours, 0.5 part to 1.0 parts of nutritive additive and dioctylis sulfosuccinas natricus; Preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.1 part of 10 parts to 18 parts of maize straws, 55 parts to 65 parts of corn flour, 4.5 parts to 6.5 parts of soy bean powders, 15 parts to 25 parts of Cottonseed Meals, 1.0 parts to 2.5 parts of stone flours, 0.5 part to 1.0 parts of nutritive additive and dioctylis sulfosuccinas natricus; More preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.08 part of 10 parts to 18 parts of maize straws, 55 parts to 65 parts of corn flour, 4.5 parts to 6.5 parts of soy bean powders, 15 parts to 25 parts of Cottonseed Meals, 1.0 parts to 2.5 parts of stone flours, 0.5 part to 1.0 parts of nutritive additive and dioctylis sulfosuccinas natricus.The feed of this composition can be the ruminant feed of 81 age in days to 120 ages in days.Above material nutrient component scope: organic matter >=92.0%, crude protein >=15.0%, cellulose≤8.0%, calcium >=0.6%, phosphorus >=0.5%.
Preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.12 part of 25 parts to 35 parts of maize straws, 50 parts to 60 parts of corn flour, 2.5 parts to 4.0 parts of soy bean powders, 8 parts to 15 parts of Cottonseed Meals, 0.8 part to 1.5 parts of stone flour, 0.5 part to 1.0 parts of nutritive additive and dioctylis sulfosuccinas natricus; Preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.1 part of 25 parts to 35 parts of maize straws, 50 parts to 60 parts of corn flour, 2.5 parts to 4.0 parts of soy bean powders, 8 parts to 15 parts of Cottonseed Meals, 0.8 part to 1.5 parts of stone flour, 0.5 part to 1.0 parts of nutritive additive and dioctylis sulfosuccinas natricus; More preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.08 part of 25 parts to 35 parts of maize straws, 50 parts to 60 parts of corn flour, 2.5 parts to 4.0 parts of soy bean powders, 8 parts to 15 parts of Cottonseed Meals, 0.8 part to 1.5 parts of stone flour, 0.5 part to 1.0 parts of nutritive additive and dioctylis sulfosuccinas natricus.The feed of this composition can be the ruminant feed of 121 age in days to 240 ages in days.Above material nutrient component scope: organic matter >=91.0%, crude protein >=12.0%, cellulose≤12.0%, calcium >=0.7%, phosphorus >=0.4%.
Preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.12 part of 60 parts to 75 parts of maize straws, 10 parts to 25 parts of corn flour, 7 parts to 15 parts of Cottonseed Meals, 2 parts to 5 parts of rapeseed dregs, 0.7 part to 2.5 parts, urea, 1.3 parts to 3.4 parts of nutritive additives and dioctylis sulfosuccinas natricus; Preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.05 to 0.1 part of 60 parts to 75 parts of maize straws, 10 parts to 25 parts of corn flour, 7 parts to 15 parts of Cottonseed Meals, 2 parts to 5 parts of rapeseed dregs, 0.7 part to 2.5 parts, urea, 1.3 parts to 3.4 parts of nutritive additives and dioctylis sulfosuccinas natricus; More preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.08 part of 60 parts to 75 parts of maize straws, 10 parts to 25 parts of corn flour, 7 parts to 15 parts of Cottonseed Meals, 2 parts to 5 parts of rapeseed dregs, 0.7 part to 2.5 parts, urea, 1.3 parts to 3.4 parts of nutritive additives and dioctylis sulfosuccinas natricus.The feed of this composition can be ruminant feed more than 241 ages in days.Above material nutrient component scope: organic matter >=85.0%, crude protein >=12.0%, cellulose≤32.0%, hemicellulose≤14.0%, calcium >=0.7%, phosphorus >=0.3%.
Preferably, in above-mentioned feed, described nutritive additive is selected from one or more in fish meal, crude salt, trace element/vitamin(e) supplement, sodium sulphate and betaine.
Preferably, described ruminant is selected from sheep, ox or camel; Be preferably sheep.
On the other hand, the invention provides the method for breeding of a kind of ruminant, this method for breeding carries out in the steps below: the consumption that adds hello dioctylis sulfosuccinas natricus to ruminant is that 0.4g/kg daily ration is to 1.2g/kg daily ration, be preferably 0.4g/kg daily ration to 1.0g/kg daily ration, more preferably 0.4g/kg daily ration is to 0.8g/kg daily ration, feeding method is in daily ration, to feed to ruminant for the dioctylis sulfosuccinas natricus of requirement is mixed at feed, and every natural gift secondary adds.
Preferably, above-mentioned method for breeding carries out in the steps below: the ruminant of 40 age in days to 80 ages in days is fed following feed: by parts by weight of raw materials, feed comprises or is comprised of following component: 0.04 to 0.12 part of 4 parts to 5 parts of maize straws, 58 parts to 65 parts of corn flour, 6.5 parts to 8.5 parts of soy bean powders, 20 parts to 24 parts of Cottonseed Meals, 2.5 parts to 3.5 parts of stone flours, 0.2 part to 1.3 parts of nutritive additive and dioctylis sulfosuccinas natricus; Preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.05 to 0.1 part of 4 parts to 5 parts of maize straws, 58 parts to 65 parts of corn flour, 6.5 parts to 8.5 parts of soy bean powders, 20 parts to 24 parts of Cottonseed Meals, 2.5 parts to 3.5 parts of stone flours, 0.2 part to 1.3 parts of nutritive additive and dioctylis sulfosuccinas natricus; More preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.08 part of 4 parts to 5 parts of maize straws, 58 parts to 65 parts of corn flour, 6.5 parts to 8.5 parts of soy bean powders, 20 parts to 24 parts of Cottonseed Meals, 2.5 parts to 3.5 parts of stone flours, 0.2 part to 1.3 parts of nutritive additive and dioctylis sulfosuccinas natricus; Add the consumption of feeding dioctylis sulfosuccinas natricus and be 0.4g/kg daily ration to 1.2g/kg daily ration, be preferably 0.4g/kg daily ration to 1.0g/kg daily ration, more preferably 0.4g/kg daily ration, to 0.8g/kg daily ration, is fed 2 times every day;
The ruminant of 81 age in days to 120 ages in days is fed following feed: by parts by weight of raw materials, feed comprises by parts by weight of raw materials, and described feed comprises or is comprised of following component: 0.04 to 0.12 part of 10 parts to 18 parts of maize straws, 55 parts to 65 parts of corn flour, 4.5 parts to 6.5 parts of soy bean powders, 15 parts to 25 parts of Cottonseed Meals, 1.0 parts to 2.5 parts of stone flours, 0.5 part to 1.0 parts of nutritive additive and dioctylis sulfosuccinas natricus; Preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.05 to 0.1 part of 10 parts to 18 parts of maize straws, 55 parts to 65 parts of corn flour, 4.5 parts to 6.5 parts of soy bean powders, 15 parts to 25 parts of Cottonseed Meals, 1.0 parts to 2.5 parts of stone flours, 0.5 part to 1.0 parts of nutritive additive and dioctylis sulfosuccinas natricus; More preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.08 part of 10 parts to 18 parts of maize straws, 55 parts to 65 parts of corn flour, 4.5 parts to 6.5 parts of soy bean powders, 15 parts to 25 parts of Cottonseed Meals, 1.0 parts to 2.5 parts of stone flours, 0.5 part to 1.0 parts of nutritive additive and dioctylis sulfosuccinas natricus; Add the consumption of feeding dioctylis sulfosuccinas natricus and be 0.4g/kg daily ration to 1.2g/kg daily ration, be preferably 0.4g/kg daily ration to 1.0g/kg daily ration, more preferably 0.4g/kg daily ration, to 0.8g/kg daily ration, is fed 2 times every day;
The ruminant of 121 age in days to 240 ages in days is fed following feed: by parts by weight of raw materials, described feed comprises or is comprised of following component: 0.04 to 0.12 part of 25 parts to 35 parts of maize straws, 50 parts to 60 parts of corn flour, 2.5 parts to 4.0 parts of soy bean powders, 8 parts to 15 parts of Cottonseed Meals, 0.8 part to 1.5 parts of stone flour, 0.5 part to 1.0 parts of nutritive additive and dioctylis sulfosuccinas natricus; Preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.05 to 0.1 part of 25 parts to 35 parts of maize straws, 50 parts to 60 parts of corn flour, 2.5 parts to 4.0 parts of soy bean powders, 8 parts to 15 parts of Cottonseed Meals, 0.8 part to 1.5 parts of stone flour, 0.5 part to 1.0 parts of nutritive additive and dioctylis sulfosuccinas natricus; More preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.08 part of 25 parts to 35 parts of maize straws, 50 parts to 60 parts of corn flour, 2.5 parts to 4.0 parts of soy bean powders, 8 parts to 15 parts of Cottonseed Meals, 0.8 part to 1.5 parts of stone flour, 0.5 part to 1.0 parts of nutritive additive and dioctylis sulfosuccinas natricus; Add the consumption of feeding dioctylis sulfosuccinas natricus and be 0.4g/kg daily ration to 1.2g/kg daily ration, be preferably 0.4g/kg daily ration to 1.0g/kg daily ration, more preferably 0.4g/kg daily ration, to 0.8g/kg daily ration, is fed 2 times every day;
Ruminants more than 241 ages in days is fed following feed: by parts by weight of raw materials, described feed comprises or is comprised of following component: 0.04 to 0.12 part of 60 parts to 75 parts of maize straws, 10 parts to 25 parts of corn flour, 7 parts to 15 parts of Cottonseed Meals, 2 parts to 5 parts of rapeseed dregs, 0.7 part to 2.5 parts, urea, 1.3 parts to 3.4 parts of nutritive additives and dioctylis sulfosuccinas natricus; Preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.05 to 0.1 part of 60 parts to 75 parts of maize straws, 10 parts to 25 parts of corn flour, 7 parts to 15 parts of Cottonseed Meals, 2 parts to 5 parts of rapeseed dregs, 0.7 part to 2.5 parts, urea, 1.3 parts to 3.4 parts of nutritive additives and dioctylis sulfosuccinas natricus; More preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.08 part of 60 parts to 75 parts of maize straws, 10 parts to 25 parts of corn flour, 7 parts to 15 parts of Cottonseed Meals, 2 parts to 5 parts of rapeseed dregs, 0.7 part to 2.5 parts, urea, 1.3 parts to 3.4 parts of nutritive additives and dioctylis sulfosuccinas natricus; Add the consumption of feeding dioctylis sulfosuccinas natricus and be 0.4g/kg daily ration to 1.2g/kg daily ration, be preferably 0.4g/kg daily ration to 1.0g/kg daily ration, more preferably 0.4g/kg daily ration, to 0.8g/kg daily ration, is fed 2 times every day.
Preferably, in above-mentioned method for breeding, described nutritive additive is selected from one or more in fish meal, crude salt, trace element/vitamin(e) supplement, sodium sulphate and betaine.
Preferably, in above-mentioned method for breeding, described ruminant is selected from sheep, ox or camel; Be preferably sheep.
Another aspect, the invention provides the application of described feed in the method for breeding of raising ruminant.Preferably, described ruminant is selected from sheep, ox or camel; Be preferably sheep.
Compared with prior art, the present invention at least has following beneficial effect:
Adopt the present invention to add after the feed of dioctylis sulfosuccinas natricus, the free choice feeding amount of sheep increases; The cud protozoon quantity of sheep reduces, and the rumen bacteria quantity of sheep increases; Arrive the significantly increases such as amino acid of small intestine of sheep; The nutriments such as amino acid that small intestine of sheep is digested and assimilated significantly increase; The Slaughter of sheep significantly improves.
The consumption that the present invention adds hello dioctylis sulfosuccinas natricus is that 0.4g/kg daily ration is to 1.2g/kg daily ration, be preferably 0.4g/kg daily ration to 1.0g/kg daily ration, more preferably 0.4g/kg daily ration is to 0.8g/kg daily ration, adds and feeds the consumption of dioctylis sulfosuccinas natricus the too high and too low effect that all can not reach.
The specific embodiment
Below in conjunction with embodiment, the present invention is described further.These embodiment are intended to help to set forth the content of invention rather than limit the scope of the invention.
Embodiment 1: add feed various dose dioctylis sulfosuccinas natricus on sheep search for food, the impact of cud protozoon and bacterial number
One, materials and methods
1, experimental animal and experimental design
4 of the cold sheep rams of little tail of choosing the about 50kg of body weight, 2 years old age, having installed permanence lymphoma stomach fistulization pipe, under daily ration (essence is thick than 30: 70) condition, by 4 * 4 Latin square designs, add respectively and feed 0,0.4,0.8 and 4.0 gram of dioctylis sulfosuccinas natricus/Kg daily ration (dry meter), to study to add, feed the impact of dioctylis sulfosuccinas natricus on sheep free choice feeding amount, rumen fluid protozoon and bacterial number etc.
Each issue test totally 18 days, wherein 14 days preliminary trial periods, just tries the phase (taking rumen fluid) 4 days.In the formal phase, every day before feeding (0 hour) and feed after within 1.5,3,5,7 and 10 hours, take rumen fluid.The rumen fluid sample of every sheep every time point every day is preserved separately, measures separately.
Measure the free choice feeding amount (6 days) of each issue of every sheep.
2, animal diets and feeding and management
The composition of daily ration and trophic level are in Table 1.
Composition and the trophic level (%, dry meter) of table 1 test daily ration
Note: 1, in upper table, nutritional labeling is measured value;
2, in upper table, crude salt, stone flour and trace element/vitamin(e) supplement are nutritive additive.
Two, results and analysis
1, add and feed the impact of dioctylis sulfosuccinas natricus on sheep free choice feeding amount
As shown in table 2, under the thick daily ration condition than 30: 70 of essence, add and feed dioctylis sulfosuccinas natricus 0.4g/kg daily ration and 0.8g/kg daily ration, total feed intake of sheep increases respectively 13.2% (P < 0.01) and 30.7% (P < 0.01) than contrast, the feed intake of maize straw also increases respectively 13.4% (P < 0.01) and 26.2% (P < 0.01), shows to add the effect that appropriate dioctylis sulfosuccinas natricus has increase sheep feed intake of feeding.Yet, when adding, feed dioctylis sulfosuccinas natricus dosage when larger (4.0g/kg daily ration), the free choice feeding amount of sheep decline (P < 0.01) (table 2).
Table 2 add feed dioctylis sulfosuccinas natricus on the impact of sheep free choice feeding amount (g/d, dry meter, n=4)
Note: the data upper right corner indicates letter and represents respectively the significance of difference between each group of same daily ration.The different significant differences (P < 0.05) that represent of lowercase, the different difference extremely significantly (P < 0.01) that represents of capitalization.Lower same.
2, add and feed the impact of dioctylis sulfosuccinas natricus on sheep rumen liquid protozoon quantity
As shown in table 3, the protozoon quantity of sheep in the 3h rumen fluid of feeding arrives minimum point, increases gradually afterwards, take interior caterpillar as main in protozoon.Adding hello dioctylis sulfosuccinas natricus significantly reduces the protozoon quantity of sheep rumen liquid, and increase and reduce with dosage effect, 0.4,0.8 with during 4.0g/kg daily ration, cud protozoon has reduced respectively 33.9% (P < 0.01), 54.2% (P < 0.01) and 86.3% (P < 0.01) than contrast, and its medium caterpillar has reduced respectively 38.8% (P < 0.01), 52.0% (P < 0.01) and 90.8% (P < 0.01); Interior caterpillar has reduced respectively 33.5% (P < 0.01), 54.1% (P < 0.01) and 86.3% (P < 0.01); Bistrichiasis worm has reduced respectively 32.3% (P < 0.01), 58.5% (P < 0.01) and 76.9% (P < 0.01).Yet, add and feed the obviously change (table 3) of rule generation that dioctylis sulfosuccinas natricus does not make cud protozoon quantity change with feeding time.
Table 3 adds feeds the impact (* 10 of dioctylis sulfosuccinas natricus (gram/sheep/day) on sheep rumen liquid protozoon quantity
5/ mL, n=4)
3, add and feed the impact of dioctylis sulfosuccinas natricus on sheep rumen liquid bacterial content
As shown in table 4, no matter rumen fluid bacterial number is sum or escherichia coli, dialister bacterium etc., all along with feeding time, is fluctuation state, and basic law is that after searching for food, quantity reduces, and increases afterwards again, then reduces.Sheep is added the effect to rumen bacteria after dioctylis sulfosuccinas natricus of feeding, depend on and add the dosage of feeding, feed 0.4 and during 0.8g/kg daily ration adding, rumen bacteria sum increases respectively 33.6% (P < 0.01) and 46.9% (P < 0.01) than contrast, and when 4.0g/kg daily ration larger dose, rumen bacteria sum but reduces by 23.5% (P < 0.01).Add feed cud dialister bacterium after dioctylis sulfosuccinas natricus, coccus and the new variation of crescent moon bacterium quantity and the Changing Pattern of rumen bacteria sum basic identical, when lower dioctylis sulfosuccinas natricus dosage, increase, when higher dosage, reduce.Feed 0.4,0.8 and during 4.0g/kg daily ration dioctylis sulfosuccinas natricus adding, dialister bacterium quantity is respectively 190.9% (P < 0.01), 241.8% (P < 0.01) and 61.9% (the P < 0.01) of control group; Coccus is respectively 130.4% (P < 0.01), 141.5% (P < 0.01) and 78.0% (the P < 0.01) of control group; New crescent moon bacterium is respectively 0.0% (P > 0.05), 103.6% (P < 0.05) and 51.8% (the P < 0.01) of control group.Yet, escherichia coli quantity constantly reduces along with the increase of dioctylis sulfosuccinas natricus dosage, feed 0.4,0.8 and during 4.0g/kg daily ration dioctylis sulfosuccinas natricus adding, be respectively 59.3% (P < 0.01), 40.7% (P < 0.01) and 16.7% (the P < 0.01) of control group.
Table 4 adds feeds the impact (* 10 of dioctylis sulfosuccinas natricus (gram/sheep/day) on sheep rumen liquid bacterial number
9/ mL, n=4)
4, add and feed the impact of dioctylis sulfosuccinas natricus on sheep rumen liquid pH
From table 5, rumen fluid ph changes along with feeding time presents fluctuation shape, and mobility scale is 6.40～6.96.Feed dioctylis sulfosuccinas natricus 0.0 (control group), 0.4 and during 0.8g/kg daily ration adding, rumen fluid ph is on average respectively 6.60 ± 0.08,6.55 ± 0.10 and 6.58 ± 0.09, with control group than all without significant difference (P > 0.05).Yet, when adding, feeding dioctylis sulfosuccinas natricus dosage when larger (4.0g/kg daily ration), pH raises (P < 0.01), average out to 6.81 ± 0.08.But add, feed the obviously change of rule generation that dioctylis sulfosuccinas natricus does not make rumen fluid ph change with feeding time.
Table 5 adds feeds dioctylis sulfosuccinas natricus to the impact of sheep rumen liquid pH (n=4)
5, add and feed the impact of dioctylis sulfosuccinas natricus on sheep rumen liquid ammonia nitrogen concentration
From table 6, the increase that the ammonia nitrogen concentration of sheep rumen liquid adds hello dosage with dioctylis sulfosuccinas natricus reduces.0.4,0.8 with reduced respectively 24.1% (P < 0.01), 35.8% (P < 0.01) and 65.8% (P < 0.01) than contrast during 4.0g/kg daily ration.But add, feed the obviously change of rule generation that dioctylis sulfosuccinas natricus does not make cud ammonia nitrogen concentration change with feeding time.
Table 6 adds feeds the impact (mg/100mL, n=4) of dioctylis sulfosuccinas natricus on sheep rumen liquid ammonia nitrogen concentration
6, add the impact of feeding dioctylis sulfosuccinas natricus and turnover rate long-pending on sheep rumen liquid
From table 7, the long-pending mobility scale of sheep rumen liquid is at 5.42～5.46L, sheep rumen liquid turnover rate mobility scale is at 0.31～0.42L/h, and that adds the Rumen volume of feeding each group of dioctylis sulfosuccinas natricus and turnover rate and control group compares difference not significantly (P > 0.05).
Table 7 adds the impact (L, L/h, n=4) of feeding aerosol and turnover rate long-pending on sheep rumen liquid
Note: the data upper right corner indicates letter and represents respectively the significance of difference between each group of same column.Indicate and represent difference not remarkable (P > 0.05).
Embodiment 2: add and feed the impact of dioctylis sulfosuccinas natricus on the cold lamb sheep growth of little tail and digestion and metabolism
One, materials and methods
1, experimental animal and experimental design
Successively choose 20 40 ages in days, the cold lamb sheep of male little tail that body condition is good, average weight 8.49 ± 0.51Kg, is divided into 5 groups at random, and 4 every group, individual pen (cage) is raised.During 40～240 ages in days, give 1,2,3,4 and 5 group add respectively feed various dose dioctylis sulfosuccinas natricus (0.0,0.4,0.8,1.2,1.6g/Kg daily ration, dry meter), to study to add, feed the impact of dioctylis sulfosuccinas natricus on lamb free choice feeding amount and growth performance.Choose the test group of feed intake and growth performance the best simultaneously, take without adding hello lamb is control group, respectively at 70～80 ages in days, 110～120 ages in days and 170～180 ages in days, carry out digest supersession test three times, impact with research dioctylis sulfosuccinas natricus on lamb digestion and metabolism, and at 240 ages in days, above two groups of lambs are carried out to slaughter determining, to study to add, feed the impact of dioctylis sulfosuccinas natricus on lamb Slaughter.
2, the raising of experimental animal and management
Basic daily ration composition and the trophic level of different days lamb are as shown in table 8.
The daily ration of table 840～240 days old lamb forms and trophic level (%, dry meter)
Note: in upper table, fish meal, crude salt and trace element/vitamin(e) supplement are nutritive additive
Two, results and analysis
1, add and feed the impact of various dose dioctylis sulfosuccinas natricus on the cold lamb sheep free choice feeding amount of little tail
As shown in table 9, add respectively to different days lamb to feed 0,0.4,0.8,1.2 and the dioctylis sulfosuccinas natricus of 1.6g/Kg daily ration (dry meter), its dry or organic matter feed intake are the situation that first increases, reduces with dosage, in its maximum feed intake of different days lamb, all appear at and add while feeding 0.8g dioctylis sulfosuccinas natricus/Kg daily ration (dry meter), average dry matter intake increases by 15.1% (P < 0.01).The continuation of feeding amount along with dioctylis sulfosuccinas natricus increases, and the free choice feeding amount of lamb reduces gradually, but when maximum dose level 1.6g dioctylis sulfosuccinas natricus/Kg daily ration, still adds with nothing feed substantially suitable, there is no the inhibition situation of searching for food.At 40～80,81～120 and 121～240 ages in days, add and feed dioctylis sulfosuccinas natricus, the maximum feed intake of lamb dry increases respectively 10.7% (P < 0.01), 14.8% (P < 0.01) and 15.7% (P < 0.01), shows that dioctylis sulfosuccinas natricus is better in the effect of larger days old lamb increase free choice feeding amount.
Table 9 add feed various dose dioctylis sulfosuccinas natricus on the impact of the public lamb free choice feeding of the cold sheep of little tail amount (dry, gram/sheep/day, n=4)
Note: in (1) table, " full phase " row refers to average daily feed intake during 40～240 ages in days.(2) sign letter in the data upper right corner represents that respectively same column is with the significance of difference between age in days different disposal, wherein lowercase is different, represent significant difference (P < 0.05), capitalization is different, represents difference extremely significantly (P < 0.01).Lower same.
2, add and feed the impact of various dose dioctylis sulfosuccinas natricus on the cold lamb sheep body weight gains of little tail
As shown in table 10, add respectively to different days lamb to feed 0.4,0.8,1.2 and the dioctylis sulfosuccinas natricus of 1.6g/Kg daily ration (dry meter), the average daily gain of lamb during 40～240 ages in days is than improving respectively 7.1% (P < 0.01) without adding the control group of feeding, 15.6% (P < 0.01), 2.6% (P > 0.05) and 0.8% (P > 0.05), show that dioctylis sulfosuccinas natricus is different with dosage on the impact of lamb body weight gains, goodly add that to feed dosage be 0.8g/Kg daily ration (dry meter).Lamb the highest weight average that increases day by day during 40～80,81～120 and 121～240 ages in days appears at and adds while feeding 0.8g/Kg daily ration, than control group, increase by 14.2% (P < 0.01), 7.0% (P < 0.01) and 15.1% (P < 0.01) respectively, but do not show the variation with age in days.
Table 10 add feed various dose dioctylis sulfosuccinas natricus on the impact of the public lamb daily gain of the cold sheep of little tail (Kg, gram/sheep/day, n=4)
3, add and feed the impact of dioctylis sulfosuccinas natricus on the cold lamb sheep Digestibility of little tail
Using do not add feed the 1st group of dioctylis sulfosuccinas natricus as a control group, add the highest the 3rd group of free choice feeding amount and body weight gains after feeding as test group, at 78～80,110～120 and 170～180 ages in days, carry out digest supersession test, its (apparent) digestibility is in Table 11.Add the dry of feeding dioctylis sulfosuccinas natricus lamb, organic matter and total energy digestibility be average 4.6% (the P > 0.05) that improve respectively, 2.9% (P > 0.05) and 3.0% (P > 0.05), crude protein, calcium, on average improve respectively 14.2% (P < 0.01) with the apparent digestibility of phosphorus, 6.8% (P < 0.05) and 21.5% (P < 0.01), cellulose and hemicellulose digestibility be average 10.3% (P < 0.01) and 4.1% (the P < 0.05) of improving respectively.Show to add and feed the digestibility that aerosol is improved the trend, particularly crude protein of digestibility, but the trend changing with age in days is totally seen not obvious.
Table 11 adds feeds the impact (%, n=4) of dioctylis sulfosuccinas natricus on public lamb daily ration (apparent) digestibility of the cold sheep of little tail
Note: the data upper right corner indicates letter and represents that respectively colleague is with the significance of difference between age in days different disposal, wherein lowercase is different, represent significant difference (P < 0.05), capitalization is different, represents difference extremely significantly (P < 0.01).Lower same.
As shown in table 12, add hello dioctylis sulfosuccinas natricus and make average dry of full phase of lamb, organic matter, total energy and crude protein digestion amount increase respectively by 19.2% (P < 0.01), 17.5% (P < 0.01), 17.9% (P < 0.01) and 32.8% (P < 0.01), calcium, phosphorus digestion amount increases respectively 22.2% (P < 0.01) and 47.6% (P < 0.01), cellulose and hemicellulose digestion amount increase respectively 19.7% (P < < 0.01) and 16.8% (P < < 0.01), show to add the nutrition supply that hello dioctylis sulfosuccinas natricus has increased lamb.70～80, during 110～120 and 170～180 ages in days, adding hello dioctylis sulfosuccinas natricus makes organic matter digestion amount increase respectively by 14.6% (P < 0.01), 21.3% (P < 0.01) and 16.1% (P < 0.01), crude protein digestion amount increases respectively by 29.5% (P < 0.01), 28.5% (P < 0.01) and 41.5% (P < 0.01), cellulose digestion amount increases respectively by 23.5% (P < 0.01), 20.1% (P < 0.01) and 18.4% (P < 0.01), show that dioctylis sulfosuccinas natricus has effect extremely significantly in each age in days stage, but the trend changing with age in days is not obvious.
Table 12 add feed dioctylis sulfosuccinas natricus on the impact of the public lamb Digestibility of the cold sheep of little tail amount (gram/sheep/day, million Jiao/sheep/day, n=4)
4, add and feed the impact of dioctylis sulfosuccinas natricus on cold lamb sheep nitrogen, calcium and phosphorus metabolism of little tail
As shown in table 13, adding hello dioctylis sulfosuccinas natricus makes lamb nitrogen reservation amount increase by 29.8% (P < 0.01), nitrogen retention rate improves 15.6% (P < 0.01), increase more in the situation that taking in nitrogen, fecal nitrogen does not almost increase, and urinary nitrogen also has minimizing.From 70～80,110～120 and 170～180 three phases of age in days, nitrogen retains increases respectively 28.4% (P < 0.01), 40.3% (P < 0.01) and 19.3% (P < 0.01), show that dioctylis sulfosuccinas natricus has positive effect in each age in days stage, but the trend changing with age in days is not obvious.
Table 13 adds feeds dioctylis sulfosuccinas natricus to the impact of the public lamb nitrogen metabolism of the cold sheep of little tail (gram/sheep/day, %, n=4)
As shown in table 14 and table 15, add while feeding dioctylis sulfosuccinas natricus, the calcium of lamb, phosphorus are taken in average 17.5% (the P < 0.01) of increasing and 18.2% (P < 0.01) respectively, but excrement calcium does not almost increase, excrement phosphorus also has the trend reducing, and the reservation amount of calcium, phosphorus and retention rate all significantly or are extremely significantly increased.
Table 14 adds feeds dioctylis sulfosuccinas natricus to the impact of the public lamb calcium metabolism of the cold sheep of little tail (gram/sheep/day, %, n=4)
Table 15 adds feeds dioctylis sulfosuccinas natricus to the impact of the public lamb phosphorus metabolism of the cold sheep of little tail (gram/sheep/day, %, n=4)
5, add and feed the impact of dioctylis sulfosuccinas natricus on the cold lamb sheep Slaughter of little tail
Shown in table 16, from 40 ages in days, to lamb, add and feed dioctylis sulfosuccinas natricus (0.8g/Kg daily ration, dry meter), at 240 ages in days, control group (feeding without adding) and test group lamb are carried out to Slaughter mensuration, the results are shown in Table 18.The carcass weight of test group lamb, dressing percentage, trunk meat weight, lean meat is heavy, fat meat is heavy and eye muscle area has increased respectively 22.6% (P < 0.01), 8.6% (P < 0.05), 24.2% (P < 0.01), 32.3% (P < 0.01), 1.4% (P > 0.05) and 14.0% (P < 0.01) than control group lamb, shows to add to feed dioctylis sulfosuccinas natricus and can effectively improve the Slaughter of lamb.
Table 16 adds feeds the impact (Kg, %, n=4) of dioctylis sulfosuccinas natricus on the public lamb Slaughter of the cold sheep of little tail
Note: the data upper right corner indicates the significance of difference that letter represents respectively colleague's contrast and test bay.The different significant differences (P < 0.05) that represent of lowercase, the different difference extremely significantly (P < 0.01) that represents of capitalization.
Embodiment 3: add and feed the impact of dioctylis sulfosuccinas natricus on small intestine of sheep absorption and whole digestion and metabolism
One, materials and methods
1, experimental animal and experimental design
Choose 6 1.5 years old, the cold sheep ram of the little tail of body weight 55.3 ± 3.8Kg, install the T-shaped fistula of permanent duodenum near-end and the T-shaped fistula of terminal ileum.Containing under the coarse fodder type daily ration condition of maize straw 67%, successively add and feed 0 (control period) and 0.8g/Kg daily ration (dry meter) (experimental period) dioctylis sulfosuccinas natricus, adopt own control experimental design, measure sheep in the free choice feeding amount of each phase, arrive the nutrients quality of small intestine, nutrients quality that small intestine is digested and assimilated and the digestion and metabolism situation of sheep integral body, and calculate the digestion amount etc. of sheep glandular stomach dry etc., to study, add the effect of dioctylis sulfosuccinas natricus to Digestion And Metabolism In Sheep of feeding.
Each issue test, animal prerun 15 days, then continuous acquisition duodenum and ileum chyme sample are 4 days, carry out digest supersession test after 14 days again, collect continuously animal fecaluria and surplus material 8 days.The samples such as chyme, excrement, urine all carry out the mensuration of conventional nutritional labeling, and chyme sample also carries out Contents of Amino Acids, and Duodenal digesta also carries out microprotein assay (nucleic acid method).Duodenum and ileum chyme flow adopt endogenous lignin labelling method to measure.
2, the raising of experimental animal and management
The test composition of daily ration and trophic level be in Table 17, daily ration maize straw content 67.0%, crude protein content 12.9%.
Composition and the trophic level (%, dry meter) of table 17 test daily ration
Note: in upper table, crude salt, stone flour and trace element/vitamin are nutritive additive.
Two, results and analysis
1, add and feed the impact of dioctylis sulfosuccinas natricus on the cold sheep free choice feeding amount of little tail
Shown in table 18, in this test, the adult Han-sheep with small-tail ram that has accounted under 67% coarse fodder type daily ration condition to device permanent duodenum and ileum fistula at stalk is added and feeds dioctylis sulfosuccinas natricus by 0.8g/Kg daily ration (dry), its dry and organic free choice feeding amount increase respectively 30.1% (P < 0.01) and 26.9% (P < 0.01) in the chyme phase of collecting, in the digestion and metabolism phase, increase respectively 30.7% (P < 0.01) and 26.6% (P < 0.01), while showing to feed coarse fodder type daily ration, add hello dioctylis sulfosuccinas natricus and can improve significantly the feed intake of sheep by the utmost point.The feed intake of maize straw has also increased respectively 31.8% (P < 0.01) and 32.4% (P < 0.01) in chyme collection phase and digestion and metabolism phase.
Table 18 add feed dioctylis sulfosuccinas natricus on the impact of the cold sheep ram free choice feeding amount of little tail (gram/sheep/day, dry meter, n=6)
Note: the data upper right corner indicates letter and represents respectively the significance of difference between the same column processing same period, wherein lowercase difference represents significant differences (P < 0.05), the different difference extremely significantly (P < 0.01) that represents of capitalization.
2, add feed dioctylis sulfosuccinas natricus on the cold sheep of little tail before peptic digest and arrive the impact of enteral nutrition material
(1) add feed dioctylis sulfosuccinas natricus on the cold sheep of little tail before peptic digest and arrive the impact of small intestine dry, organic matter, crude protein etc.
Shown in table 19, add to adult Han-sheep with small-tail ram and feed dioctylis sulfosuccinas natricus, the digestion amount of glandular stomach dry, organic matter and crude protein has increased respectively 27.9% (P < 0.01), 28.4% (P < 0.01) and 21.8% (P < 0.01), the digestion amount of glandular stomach cellulose, hemicellulose has increased respectively 29.7% (P < 0.01) and 30.2% (P < 0.01), shows to add hello dioctylis sulfosuccinas natricus and has promoted ruminal digestion.
Add and feed dry, organic matter and the crude protein quantity that dioctylis sulfosuccinas natricus makes to arrive small intestine and increased respectively 10.2% (P < 0.01), 10.8% (P < 0.01) and 27.8% (P < 0.01); The microprotein and the rumen bypass protein that arrive small intestine have increased respectively 34.3% (P < 0.01) and 21.6% (P < 0.01); The cellulose, the hemicellulose amount that arrive small intestine have increased respectively 10.4% (P < 0.01) and 8.9% (P < 0.05); The calcium and the phosphorus amount that arrive small intestine have increased respectively 13.6% (P < 0.01) and 45.6% (P < 0.01), show to add hello dioctylis sulfosuccinas natricus and when increasing ruminal digestion amount, also increase the nutrients quality, particularly protein that arrives small intestine.
Table 19 add feed dioctylis sulfosuccinas natricus on the cold sheep ram of little tail before peptic digest and arrive the nutritional labelings such as small intestine dry, organic matter, crude protein impact (gram/sheep/day, n=6)
Note: the data upper right corner indicates letter and represents respectively the significance of difference between colleague's processing, wherein lowercase difference represents significant differences (P < 0.05), capitalization is different, represent difference extremely significantly (P < 0.01), without letter mark, represent difference not remarkable (P > 0.05).Lower same.
(2) add and feed dioctylis sulfosuccinas natricus to arriving the cold amino acid whose impact of small sheep intestines of little tail
Shown in table 20, adding hello dioctylis sulfosuccinas natricus makes the total amino acid amount that arrives small intestine increase by 18.3% (P < 0.01), TEAA has increased by 19.1% (P < 0.01), lysine wherein, methionine, leucine, isoleucine, valine, arginine, threonine, phenylalanine and histidine have increased respectively by 12.2% (P < 0.01), 10.6% (P < 0.05), 11.5% (P < 0.05), 19.2% (P < 0.01), 29.3% (P < 0.01), 19.0% (P < 0.01), 37.7% (P < 0.01), 21.0% (P < 0.01) and 7.6% (P > 0.05), show to add the amino acid supply of feeding dioctylis sulfosuccinas natricus increase arrival small intestine, particularly various must amino acid, except histidine, also all significantly increase.
Table 20 adds feeds dioctylis sulfosuccinas natricus and the cold sheep ram of little tail is arrived to the impact (gram/sheep/day, %, n=6) of Small intestine amino acid
3, add and feed the impact that cold small sheep intestines nutrient digestion absorbs of the little tail of dioctylis sulfosuccinas natricus
(1) add and feed the impact that dioctylis sulfosuccinas natricus is digested and assimilated the cold small sheep intestines dry of little tail, organic matter, crude protein etc.
Shown in table 21, adding hello dioctylis sulfosuccinas natricus makes dry, organic matter, crude protein that small intestine of sheep is digested and assimilated increase respectively 63.7% (P < 0.01), 61.9% (P < 0.01) and 41.7% (P < 0.01), the calcium and the phosphorus that absorb increase respectively 40.2% (P < 0.01) and 10.2% (P < 0.05), and showing to add hello dioctylis sulfosuccinas natricus has increased the nutrition supply amount to animal.And the digestion amount of small intestine hemicellulose also increases by 41.8% (P < 0.01).
Table 21 add the impact of feeding dioctylis sulfosuccinas natricus the cold sheep ram small intestine summary Nutrients Digestion of little tail being absorbed (gram/sheep/day, n=6)
(2) add and feed the impact of dioctylis sulfosuccinas natricus on the cold small sheep intestines Amino Acid Absorption of little tail
Shown in table 22, add and feed Amino Acid Absorption amount increase by 21.7% (the P < 0.01) that dioctylis sulfosuccinas natricus makes small intestine of sheep, TEAA increases by 21.6% (P < 0.01), lysine wherein, methionine, leucine, isoleucine, valine, arginine, threonine, phenylalanine and histidine increase respectively by 17.9% (P < 0.01), 40.5% (P < 0.01), 18.6% (P < 0.01), 30.8% (P < 0.01), 9.5% (P < 0.05), 27.2% (P < 0.01), 20.5% (P < 0.01), 18.0% (P < 0.01) and 24.7% (P < 0.01), show to add the amino acid supply that hello the dioctylis sulfosuccinas natricus utmost point has increased animal significantly.
From the amino acid ratio of intestinal absorption, add hello dioctylis sulfosuccinas natricus the ratio of glycine, histidine, isoleucine and methionine is significantly raise, and serine and valine ratio significantly reduce.
Table 22 adds the impact of feeding dioctylis sulfosuccinas natricus the cold sheep ram Small intestine amino acid of little tail being digested and assimilated (gram/sheep/day, %, n=6)
4, add and feed the impact of dioctylis sulfosuccinas natricus on the cold sheep Digestibility of little tail
Shown in table 23, add hello dioctylis sulfosuccinas natricus and make dry, organic matter, crude protein (apparent) digestibility of sheep improve respectively 6.8% (P < 0.05), 7.6% (P < 0.05) and 7.9% (P < 0.05), digestion amount increases respectively 38.8% (P < 0.01), 40.7% (P < 0.01) and 46.5% (P < 0.01).The apparent digestibility of calcium and phosphorus improves respectively 6.9% (P > 0.05) and 11.5% (P < 0.05), and digestion amount increases respectively 38.5% (P < 0.01) and 39.2% (P < 0.01).The digestibility of cellulose and hemicellulose improves respectively 4.4% (P < 0.05) and 5.4% (P < 0.05), and digestion amount increases respectively 38.1% (P < 0.01) and 38.8% (P < 0.01).Show to add and feed the digestibility that dioctylis sulfosuccinas natricus can improve or significantly improve Nutritional Sheep material, and the utmost point increases the digestion amount of Nutritional Sheep material significantly.
Table 23 add feed dioctylis sulfosuccinas natricus on the impact of cold sheep ram nutriment (apparent) digestibility (amount) of little tail (%, gram/sheep/sky, n=6)
5, add and feed the impact of dioctylis sulfosuccinas natricus on cold sheep nitrogen, calcium and phosphorus metabolism of little tail
Shown in table 24, the nitrogen amount of eating of adding adult Han-sheep with small-tail ram while feeding dioctylis sulfosuccinas natricus increases by 35.5% (P < 0.01), nitrogen reservation amount increases by 49.1% (P < 0.01), nitrogen retention rate increases by 10.1% (P < 0.01), shows that the nitrogen metabolism of animal is improved.Urinary nitrogen discharge ratio is suitable with control group, and fecal nitrogen discharge ratio reduces than contrast group, show that the digestibility of nitrogen improves, and utilization rate does not change.Adding hello dioctylis sulfosuccinas natricus makes calcium, the phosphorus reservation amount of sheep increase respectively 30.8% (P < 0.01) and 53.4% (P < 0.01).
Table 24 adds feeds dioctylis sulfosuccinas natricus to the impact of cold sheep ram nitrogen, calcium and phosphorus metabolism of little tail (gram/sheep/day, %, n=6)
Embodiment 4: the toxicologic study of dioctylis sulfosuccinas natricus
One, materials and methods
1, experimental animal
Healthy Kunming mouse, SPF level, body weight (20.2 ± 1.5) g, buys in Xinjiang Medicine University.
2, experimental design
The preparation of 2.1 dioctylis sulfosuccinas natricus saturated solutions
Get dioctylis sulfosuccinas natricus (the smart biological High Seience Technology Co., Ltd. of Shanghai China, purity: LR) 2.1g, put in 1000mL volumetric flask configuration dioctylis sulfosuccinas natricus saturated solution (0.0021g/mL, 28 ℃ of room temperatures).
2.2 experimental animal groupings
Get 120 of small white mouses, be divided into immediately 6 groups.Every group 20, male and female half and half.Test period 14d.Each dosage group is respectively: 0,880,1240,1630,2550 and 3650mgkg-1.
3, test data sheet
According to after each group dosage gavage, observe and respectively organize mouse diing time and quantity.
4, data are calculated
According to each dosage group mouse death rate of different component, by Bliss method, calculate.
Two, result
The oral half lethal dose of mouse (LD50) of dioctylis sulfosuccinas natricus is 1729mgkg-1 (in Table 25).
Table 25 dioctylis sulfosuccinas natricus half lethal dose data (n=20)
Claims (12)
1. add a ruminant feed of feeding dioctylis sulfosuccinas natricus, it is characterized in that, by parts by weight of raw materials, described feed comprises 0.04 part to 0.12 part of dioctylis sulfosuccinas natricus, and preferably, described feed comprises 0.04 part to 0.1 part of dioctylis sulfosuccinas natricus; More preferably, described feed comprises 0.04 part to 0.08 part of dioctylis sulfosuccinas natricus.
2. feed according to claim 1, it is characterized in that, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.12 part of 4 parts to 5 parts of maize straws, 58 parts to 65 parts of corn flour, 6.5 parts to 8.5 parts of soy bean powders, 20 parts to 24 parts of Cottonseed Meals, 2.5 parts to 3.5 parts of stone flours, 0.2 part to 1.3 parts of nutritive additive and dioctylis sulfosuccinas natricus; Preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.1 part of 4 parts to 5 parts of maize straws, 58 parts to 65 parts of corn flour, 6.5 parts to 8.5 parts of soy bean powders, 20 parts to 24 parts of Cottonseed Meals, 2.5 parts to 3.5 parts of stone flours, 0.2 part to 1.3 parts of nutritive additive and dioctylis sulfosuccinas natricus; More preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.08 part of 4 parts to 5 parts of maize straws, 58 parts to 65 parts of corn flour, 6.5 parts to 8.5 parts of soy bean powders, 20 parts to 24 parts of Cottonseed Meals, 2.5 parts to 3.5 parts of stone flours, 0.2 part to 1.3 parts of nutritive additive and dioctylis sulfosuccinas natricus.
3. feed according to claim 1, it is characterized in that, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.12 part of 10 parts to 18 parts of maize straws, 55 parts to 65 parts of corn flour, 4.5 parts to 6.5 parts of soy bean powders, 15 parts to 25 parts of Cottonseed Meals, 1.0 parts to 2.5 parts of stone flours, 0.5 part to 1.0 parts of nutritive additive and dioctylis sulfosuccinas natricus; Preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.1 part of 10 parts to 18 parts of maize straws, 55 parts to 65 parts of corn flour, 4.5 parts to 6.5 parts of soy bean powders, 15 parts to 25 parts of Cottonseed Meals, 1.0 parts to 2.5 parts of stone flours, 0.5 part to 1.0 parts of nutritive additive and dioctylis sulfosuccinas natricus; More preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.08 part of 10 parts to 18 parts of maize straws, 55 parts to 65 parts of corn flour, 4.5 parts to 6.5 parts of soy bean powders, 15 parts to 25 parts of Cottonseed Meals, 1.0 parts to 2.5 parts of stone flours, 0.5 part to 1.0 parts of nutritive additive and dioctylis sulfosuccinas natricus.
4. feed according to claim 1, it is characterized in that, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.12 part of 25 parts to 35 parts of maize straws, 50 parts to 60 parts of corn flour, 2.5 parts to 4.0 parts of soy bean powders, 8 parts to 15 parts of Cottonseed Meals, 0.8 part to 1.5 parts of stone flour, 0.5 part to 1.0 parts of nutritive additive and dioctylis sulfosuccinas natricus; Preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.1 part of 25 parts to 35 parts of maize straws, 50 parts to 60 parts of corn flour, 2.5 parts to 4.0 parts of soy bean powders, 8 parts to 15 parts of Cottonseed Meals, 0.8 part to 1.5 parts of stone flour, 0.5 part to 1.0 parts of nutritive additive and dioctylis sulfosuccinas natricus; More preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.08 part of 25 parts to 35 parts of maize straws, 50 parts to 60 parts of corn flour, 2.5 parts to 4.0 parts of soy bean powders, 8 parts to 15 parts of Cottonseed Meals, 0.8 part to 1.5 parts of stone flour, 0.5 part to 1.0 parts of nutritive additive and dioctylis sulfosuccinas natricus.
5. feed according to claim 1, it is characterized in that, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.12 part of 60 parts to 75 parts of maize straws, 10 parts to 25 parts of corn flour, 7 parts to 15 parts of Cottonseed Meals, 2 parts to 5 parts of rapeseed dregs, 0.7 part to 2.5 parts, urea, 1.3 parts to 3.4 parts of nutritive additives and dioctylis sulfosuccinas natricus; Preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.05 to 0.1 part of 60 parts to 75 parts of maize straws, 10 parts to 25 parts of corn flour, 7 parts to 15 parts of Cottonseed Meals, 2 parts to 5 parts of rapeseed dregs, 0.7 part to 2.5 parts, urea, 1.3 parts to 3.4 parts of nutritive additives and dioctylis sulfosuccinas natricus; More preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.08 part of 60 parts to 75 parts of maize straws, 10 parts to 25 parts of corn flour, 7 parts to 15 parts of Cottonseed Meals, 2 parts to 5 parts of rapeseed dregs, 0.7 part to 2.5 parts, urea, 1.3 parts to 3.4 parts of nutritive additives and dioctylis sulfosuccinas natricus.
6. according to the feed described in any one in claim 1 to 5, it is characterized in that, described nutritive additive is selected from one or more in fish meal, crude salt, trace element/vitamin(e) supplement, sodium sulphate and betaine.
7. according to the feed described in any one in claim 1 to 6, it is characterized in that, described ruminant is selected from sheep, ox or camel; Be preferably sheep.
8. the method for breeding of a ruminant, it is characterized in that, carry out in the steps below: the consumption that adds hello dioctylis sulfosuccinas natricus to ruminant is that 0.4g/kg daily ration is to 1.2g/kg daily ration, be preferably 0.4g/kg daily ration to 1.0g/kg daily ration, more preferably 0.4g/kg daily ration is to 0.8g/kg daily ration, feeding method is in daily ration, to feed to ruminant for the dioctylis sulfosuccinas natricus of requirement is mixed at feed, and every natural gift secondary adds.
9. method for breeding according to Claim 8, it is characterized in that, carry out in the steps below: the ruminant of 40 age in days to 80 ages in days is fed following feed: by parts by weight of raw materials, feed comprises or is comprised of following component: 0.04 to 0.12 part of 4 parts to 5 parts of maize straws, 58 parts to 65 parts of corn flour, 6.5 parts to 8.5 parts of soy bean powders, 20 parts to 24 parts of Cottonseed Meals, 2.5 parts to 3.5 parts of stone flours, 0.2 part to 1.3 parts of nutritive additive and dioctylis sulfosuccinas natricus; Preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.05 to 0.1 part of 4 parts to 5 parts of maize straws, 58 parts to 65 parts of corn flour, 6.5 parts to 8.5 parts of soy bean powders, 20 parts to 24 parts of Cottonseed Meals, 2.5 parts to 3.5 parts of stone flours, 0.2 part to 1.3 parts of nutritive additive and dioctylis sulfosuccinas natricus; More preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.08 part of 4 parts to 5 parts of maize straws, 58 parts to 65 parts of corn flour, 6.5 parts to 8.5 parts of soy bean powders, 20 parts to 24 parts of Cottonseed Meals, 2.5 parts to 3.5 parts of stone flours, 0.2 part to 1.3 parts of nutritive additive and dioctylis sulfosuccinas natricus; Add the consumption of feeding dioctylis sulfosuccinas natricus and be 0.4g/kg daily ration to 1.2g/kg daily ration, be preferably 0.4g/kg daily ration to 1.0g/kg daily ration, more preferably 0.4g/kg daily ration, to 0.8g/kg daily ration, is fed 2 times every day;
The ruminant of 81 age in days to 120 ages in days is fed following feed: by parts by weight of raw materials, feed comprises by parts by weight of raw materials, and described feed comprises or is comprised of following component: 0.04 to 0.12 part of 10 parts to 18 parts of maize straws, 55 parts to 65 parts of corn flour, 4.5 parts to 6.5 parts of soy bean powders, 15 parts to 25 parts of Cottonseed Meals, 1.0 parts to 2.5 parts of stone flours, 0.5 part to 1.0 parts of nutritive additive and dioctylis sulfosuccinas natricus; Preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.05 to 0.1 part of 10 parts to 18 parts of maize straws, 55 parts to 65 parts of corn flour, 4.5 parts to 6.5 parts of soy bean powders, 15 parts to 25 parts of Cottonseed Meals, 1.0 parts to 2.5 parts of stone flours, 0.5 part to 1.0 parts of nutritive additive and dioctylis sulfosuccinas natricus; More preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.08 part of 10 parts to 18 parts of maize straws, 55 parts to 65 parts of corn flour, 4.5 parts to 6.5 parts of soy bean powders, 15 parts to 25 parts of Cottonseed Meals, 1.0 parts to 2.5 parts of stone flours, 0.5 part to 1.0 parts of nutritive additive and dioctylis sulfosuccinas natricus; Add the consumption of feeding dioctylis sulfosuccinas natricus and be 0.4g/kg daily ration to 1.2g/kg daily ration, be preferably 0.4g/kg daily ration to 1.0g/kg daily ration, more preferably 0.4g/kg daily ration, to 0.8g/kg daily ration, is fed 2 times every day;
The ruminant of 121 age in days to 240 ages in days is fed following feed: by parts by weight of raw materials, described feed comprises or is comprised of following component: 0.04 to 0.12 part of 25 parts to 35 parts of maize straws, 50 parts to 60 parts of corn flour, 2.5 parts to 4.0 parts of soy bean powders, 8 parts to 15 parts of Cottonseed Meals, 0.8 part to 1.5 parts of stone flour, 0.5 part to 1.0 parts of nutritive additive and dioctylis sulfosuccinas natricus; Preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.05 to 0.1 part of 25 parts to 35 parts of maize straws, 50 parts to 60 parts of corn flour, 2.5 parts to 4.0 parts of soy bean powders, 8 parts to 15 parts of Cottonseed Meals, 0.8 part to 1.5 parts of stone flour, 0.5 part to 1.0 parts of nutritive additive and dioctylis sulfosuccinas natricus; More preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.08 part of 25 parts to 35 parts of maize straws, 50 parts to 60 parts of corn flour, 2.5 parts to 4.0 parts of soy bean powders, 8 parts to 15 parts of Cottonseed Meals, 0.8 part to 1.5 parts of stone flour, 0.5 part to 1.0 parts of nutritive additive and dioctylis sulfosuccinas natricus; Add the consumption of feeding dioctylis sulfosuccinas natricus and be 0.4g/kg daily ration to 1.2g/kg daily ration, be preferably 0.4g/kg daily ration to 1.0g/kg daily ration, more preferably 0.4g/kg daily ration, to 0.8g/kg daily ration, is fed 2 times every day;
Ruminants more than 241 ages in days is fed following feed: by parts by weight of raw materials, described feed comprises or is comprised of following component: 0.04 to 0.12 part of 60 parts to 75 parts of maize straws, 10 parts to 25 parts of corn flour, 7 parts to 15 parts of Cottonseed Meals, 2 parts to 5 parts of rapeseed dregs, 0.7 part to 2.5 parts, urea, 1.3 parts to 3.4 parts of nutritive additives and dioctylis sulfosuccinas natricus; Preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.05 to 0.1 part of 60 parts to 75 parts of maize straws, 10 parts to 25 parts of corn flour, 7 parts to 15 parts of Cottonseed Meals, 2 parts to 5 parts of rapeseed dregs, 0.7 part to 2.5 parts, urea, 1.3 parts to 3.4 parts of nutritive additives and dioctylis sulfosuccinas natricus; More preferably, by parts by weight of raw materials, described feed comprises or is composed of the following components: 0.04 to 0.08 part of 60 parts to 75 parts of maize straws, 10 parts to 25 parts of corn flour, 7 parts to 15 parts of Cottonseed Meals, 2 parts to 5 parts of rapeseed dregs, 0.7 part to 2.5 parts, urea, 1.3 parts to 3.4 parts of nutritive additives and dioctylis sulfosuccinas natricus; Add the consumption of feeding dioctylis sulfosuccinas natricus and be 0.4g/kg daily ration to 1.2g/kg daily ration, be preferably 0.4g/kg daily ration to 1.0g/kg daily ration, more preferably 0.4g/kg daily ration, to 0.8g/kg daily ration, is fed 2 times every day.
10. method for breeding according to claim 9, is characterized in that, described nutritive additive is selected from one or more in fish meal, crude salt, trace element/vitamin(e) supplement, sodium sulphate and betaine.
11. according to the method for breeding described in claim 9 or 10, it is characterized in that, described ruminant is selected from sheep, ox or camel; Be preferably sheep.
The application of feed in 12. claims 1 to 8 described in any one in the method for breeding of raising ruminant; Preferably, described ruminant is selected from sheep, ox or camel; Be preferably sheep.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410101709.0A CN103918894A (en) | 2014-03-18 | 2014-03-18 | Ruminant feed added with aerosol OT, and feeding method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410101709.0A CN103918894A (en) | 2014-03-18 | 2014-03-18 | Ruminant feed added with aerosol OT, and feeding method thereof |
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| CN101711552A (en) * | 2009-09-16 | 2010-05-26 | 雒秋江 | Breeding method of feeding non-protective amino acid to early-weaning lambs, feedstuff and preparation method thereof |
| CN101843227A (en) * | 2010-05-31 | 2010-09-29 | 雒秋江 | Feeding method of sheep oral formalin and feed thereof |
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- 2014-03-18 CN CN201410101709.0A patent/CN103918894A/en active Pending
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|---|---|---|---|---|
| CN101711552A (en) * | 2009-09-16 | 2010-05-26 | 雒秋江 | Breeding method of feeding non-protective amino acid to early-weaning lambs, feedstuff and preparation method thereof |
| CN101843227A (en) * | 2010-05-31 | 2010-09-29 | 雒秋江 | Feeding method of sheep oral formalin and feed thereof |
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Application publication date: 20140716 |